RDB07031 - ptk-luc

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

ptk-luc (#RDB07031)

A control reporter plasmid under tk promoter.

Alternative name	stock no 649
Clone info.	A control reporter plasmid under tk promoter.
Comment	168-bp BamHI-XhoI fragment derived from pBLCAT2 containing minimal herpes simplex virus thymidine kinase (tk) promoter (Niwa, H., Mol. Cell. Biol., 1526-1536, 2002)..
Vector backbone	pGL3-basic (Plasmid)
Depositor\|Developer	Niwa, Hitoshi \|

Sequence	RDB07031z.seq
Additional map
Remarks, protocol and/or map (pdf)	RDB07031p.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Mol. Cell. Biol., 22, 1526-1536 (2002)).
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Mol. Cell. Biol., 22, 1526-1536 (2002)). Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。(Mol. Cell. Biol., 22, 1526-1536 (2002)) MTAに書く付加的使用条件：本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07031	ptk-luc	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The ptk-luc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07031).

Reference section:

Niwa, H., Masui, S., Chambers, I., Smith, A.G., Miyazaki, J., Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells. Mol. Cell. Biol., 22, 1526-1536 (2002). PMID 11839818. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB07031_A9Dcp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pause site Sequence file: RDB07031_A9Dca.seq
>07031_07031_A9Dc_2-A_pause site_A02_01_ABI08.ab1 1 GTTCCATTTT CTCTATCGAT AGGTACCGAG CTCTTACGCG TGCTAGCCCG GGCTCGAGAT 61 CCGGCCCCGC CCAGCGTCTT GTCATTGGCG AATTCGAACA CGCAGATGCA GTCGGGGCGG 121 CGCGGTCCGA GGTCCACTTC GCATATTAAG GTGACGCGTG TGGCCTCGAA CACCGAGCGA 181 CCCTGCAGCG ACCCGCTTAA CAGCGTCAAC AGCGTGCCGC AGATCTCGAA GCTTGGCATT 241 CCGGTACTGT TGGTAAAGCC ACCATGGAAG ACGCCAAAAA CATAAAGAAA GGCCCGGCGC 301 CATTCTATCC GCTGGAAGAT GGAACCGCTG GAGAGCAACT GCATAAGGCT ATGAAGAGAT 361 ACGCCCTGGT TCCTGGAACA ATTGCTTTTA CAGATGCACA TATCGAGGTG GACATCACTT 421 ACGCTGAGTA CTTCGAAATG TCCGTTCGGT TGGCAGAAGC TATGAAACGA TATGGGCTGA 481 ATACAAATCA CAGAATCGTC GTATGCAGTG AAAACTCTCT TCAATTCTTT ATGCCGGTGT 541 TGGGCGCGTT ATTTATCGGA GTTGCAGTTG CGCCCGCGAA CGACATTTAT AATGAACGTG 601 AATTGCTCAA CAGTATGGGC ATTTCGCAGC CTACCGTGGT GTTCGTTTCC AAAAAGGGGT 661 TGCAAAAAAT TTTGAACGTG CAAAAAAAGC TCCCAATCAT CCAAAAAATT ATTATCATGG 721 ATTCTAAAAC GGATTACCAG GGATTTCAGT CGATGTACAC GTTCGTCACA TCTCATCTAC 781 CTCCCGGTTT TAATGAATAC GATTTTGTGC CAGAGTCCTT CGATAGGGGA CAAGACAATT 841 GCACTGATCA TGAACTCCTC TGGATCTACT GGTCTGCCTA AAGGTGTCGC TCTGCCTCAT 901 AGAACTGCCT GCGTGAGATT CTCGGCATGC CAGAAGATCC TATTTTTTGG GCAATCAAAA 961 TCATTCCGGG ATACTGGCGA TTTTTAAAGT GTTGGTTCCC ATTTCCAATC ACGGGTTTTT 1021 GGGAAATGGT TAACTTAACA CTTCCGGGAT ATTTTGGATT ATGTGGGAAT TTCGGAGTTC 1081 GGTCCTTAAA TGTATAAGAA ATTGGAAGAA GAGCTGGTTT TCTGGAGGAG CCTTCAGGAA 1141 TTACCAAGAG AAATTCCAAA GAGTGTC //
Primer: pGL3_Rv4 Sequence file: RDB07031_A9Dcb.seq
>07031_07031_A9Dc_2-A_pGL3_Rv4_B02_02_ABI08.ab1 1 GGAGAAAGGC ATGGGGGTTG AGGCTCTCAA GGGCATCGGT CGACGGATCC TTATCGATTT 61 TACCACATTT GTAGAGGTTT TACTTGCTTT AAAAAACCTC CCACACCTCC CCCTGAACCT 121 GAAACATAAA ATGAATGCAA TTGTTGTTGT TAACTTGTTT ATTGCAGCTT ATAATGGTTA 181 CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG 241 TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGCTCGAAGC GGCCGGCCGC 301 CCCGACTCTA GAATTACACG GCGATCTTTC CGCCCTTCTT GGCCTTTATG AGGATCTCTC 361 TGATTTTTCT TGCGTCGAGT TTTCCGGTAA GACCTTTCGG TACTTCGTCC ACAAACACAA 421 CTCCTCCGCG CAACTTTTTC GCGGTTGTTA CTTGACTGGC GACGTAATCC ACGATCTCTT 481 TTTCCGTCAT CGTCTTTCCG TGCTCCAAAA CAACAACGGC GGCGGGAAGT TCACCGGCGT 541 CATCGTCGGG AAGACCTGCG ACACCTGCGT CGAAGATGTT GGGGTGTTGG AGCAAGATGG 601 ATTCCAATTC AGCGGGAGCC ACCTGATAGC CTTTGTACTT AATCAGAGAC TTCAGGCGGT 661 CAACGATGAA GAAGTGTTCG TCTTCGTCCC AGTAAGCTAT GTCTCCAGAA TGTAGCCATC 721 CATCCTTGTC AATCAAGGCG TTGGTCGCTT CCGGATTGTT TACATAACCG GACATAATCA 781 TAGGACCTCT CACACACAGT TCGCCTCTTT GATTAACGCC CAGCGTTTTC CCGGTATCCA 841 GATCCACAAC CTTCGCTTCA AAAAAATGGG AACAACTTTA CCGACCGCGC CCGGGTTTAT 901 CATCCCCCTC GGGTGTAATC AGAATAGCTT GATGTAGTCC TCAGTGAGCC CATATCCTTG 961 CCTGGATACC CTGGGCCAGA TGGGACCCTC TTTGGCAACC CGCCTTTCCC CCGGACTTCC 1021 CTTAGAGAGG GGAGCCGCCA CCAGGAAGGC TATTTCCGTG TAAATTAAGA TAATTCGGTA 1081 TTGGTCATCA AGTGCTTTGC CGAGACGAGA AATAAGGTGG CCACCAGCAA AGCGCGCCAC 1141 CATG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Niwa, H., Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells. Mol. Cell. Biol., 22, 1526-1536 (2002). PMID 11839818. [link to RRC of NBRP]

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