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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

ptk-luc

A control reporter plasmid under tk promoter.

Catalog number RDB07031
Resource name ptk-luc
Alternative name stock no 649
Clone info. A control reporter plasmid under tk promoter.
Comment 168-bp BamHI-XhoI fragment derived from pBLCAT2 containing minimal herpes simplex virus thymidine kinase (tk) promoter (Niwa, H., Mol. Cell. Biol., 1526-1536, 2002)..
Vector backbone pGL3-basic (Plasmid)
Depositor|Developer Niwa, Hitoshi |
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            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Mol. Cell. Biol., 22, 1526-1536 (2002)).
            Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。(Mol. Cell. Biol., 22, 1526-1536 (2002))
            付加的提供条件 本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
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            RDB07031 ptk-luc DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB07031z.seq
            Remarks, protocol and/or map (gif) RDB07031a.gif
            Remarks, protocol and/or map (pdf) RDB07031p.pdf

            Original reference

            original Niwa, H., Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells. Mol. Cell. Biol., 22, 1526-1536 (2002). PMID 11839818.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Control Vectors (English text)
            Featured content Reguration of genes in ES cells (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB07031_A9Dcp1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pause site
            Sequence file: RDB07031_A9Dca.seq check
            >07031_07031_A9Dc_2-A_pause site_A02_01_ABI08.ab1
                1 GTTCCATTTT CTCTATCGAT AGGTACCGAG CTCTTACGCG TGCTAGCCCG GGCTCGAGAT
               61 CCGGCCCCGC CCAGCGTCTT GTCATTGGCG AATTCGAACA CGCAGATGCA GTCGGGGCGG
              121 CGCGGTCCGA GGTCCACTTC GCATATTAAG GTGACGCGTG TGGCCTCGAA CACCGAGCGA
              181 CCCTGCAGCG ACCCGCTTAA CAGCGTCAAC AGCGTGCCGC AGATCTCGAA GCTTGGCATT
              241 CCGGTACTGT TGGTAAAGCC ACCATGGAAG ACGCCAAAAA CATAAAGAAA GGCCCGGCGC
              301 CATTCTATCC GCTGGAAGAT GGAACCGCTG GAGAGCAACT GCATAAGGCT ATGAAGAGAT
              361 ACGCCCTGGT TCCTGGAACA ATTGCTTTTA CAGATGCACA TATCGAGGTG GACATCACTT
              421 ACGCTGAGTA CTTCGAAATG TCCGTTCGGT TGGCAGAAGC TATGAAACGA TATGGGCTGA
              481 ATACAAATCA CAGAATCGTC GTATGCAGTG AAAACTCTCT TCAATTCTTT ATGCCGGTGT
              541 TGGGCGCGTT ATTTATCGGA GTTGCAGTTG CGCCCGCGAA CGACATTTAT AATGAACGTG
              601 AATTGCTCAA CAGTATGGGC ATTTCGCAGC CTACCGTGGT GTTCGTTTCC AAAAAGGGGT
              661 TGCAAAAAAT TTTGAACGTG CAAAAAAAGC TCCCAATCAT CCAAAAAATT ATTATCATGG
              721 ATTCTAAAAC GGATTACCAG GGATTTCAGT CGATGTACAC GTTCGTCACA TCTCATCTAC
              781 CTCCCGGTTT TAATGAATAC GATTTTGTGC CAGAGTCCTT CGATAGGGGA CAAGACAATT
              841 GCACTGATCA TGAACTCCTC TGGATCTACT GGTCTGCCTA AAGGTGTCGC TCTGCCTCAT
              901 AGAACTGCCT GCGTGAGATT CTCGGCATGC CAGAAGATCC TATTTTTTGG GCAATCAAAA
              961 TCATTCCGGG ATACTGGCGA TTTTTAAAGT GTTGGTTCCC ATTTCCAATC ACGGGTTTTT
             1021 GGGAAATGGT TAACTTAACA CTTCCGGGAT ATTTTGGATT ATGTGGGAAT TTCGGAGTTC
             1081 GGTCCTTAAA TGTATAAGAA ATTGGAAGAA GAGCTGGTTT TCTGGAGGAG CCTTCAGGAA
            1141 TTACCAAGAG AAATTCCAAA GAGTGTC
            //
            Primer: pGL3_Rv4
            Sequence file: RDB07031_A9Dcb.seq check
            >07031_07031_A9Dc_2-A_pGL3_Rv4_B02_02_ABI08.ab1
                1 GGAGAAAGGC ATGGGGGTTG AGGCTCTCAA GGGCATCGGT CGACGGATCC TTATCGATTT
               61 TACCACATTT GTAGAGGTTT TACTTGCTTT AAAAAACCTC CCACACCTCC CCCTGAACCT
              121 GAAACATAAA ATGAATGCAA TTGTTGTTGT TAACTTGTTT ATTGCAGCTT ATAATGGTTA
              181 CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG
              241 TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGCTCGAAGC GGCCGGCCGC
              301 CCCGACTCTA GAATTACACG GCGATCTTTC CGCCCTTCTT GGCCTTTATG AGGATCTCTC
              361 TGATTTTTCT TGCGTCGAGT TTTCCGGTAA GACCTTTCGG TACTTCGTCC ACAAACACAA
              421 CTCCTCCGCG CAACTTTTTC GCGGTTGTTA CTTGACTGGC GACGTAATCC ACGATCTCTT
              481 TTTCCGTCAT CGTCTTTCCG TGCTCCAAAA CAACAACGGC GGCGGGAAGT TCACCGGCGT
              541 CATCGTCGGG AAGACCTGCG ACACCTGCGT CGAAGATGTT GGGGTGTTGG AGCAAGATGG
              601 ATTCCAATTC AGCGGGAGCC ACCTGATAGC CTTTGTACTT AATCAGAGAC TTCAGGCGGT
              661 CAACGATGAA GAAGTGTTCG TCTTCGTCCC AGTAAGCTAT GTCTCCAGAA TGTAGCCATC
              721 CATCCTTGTC AATCAAGGCG TTGGTCGCTT CCGGATTGTT TACATAACCG GACATAATCA
              781 TAGGACCTCT CACACACAGT TCGCCTCTTT GATTAACGCC CAGCGTTTTC CCGGTATCCA
              841 GATCCACAAC CTTCGCTTCA AAAAAATGGG AACAACTTTA CCGACCGCGC CCGGGTTTAT
              901 CATCCCCCTC GGGTGTAATC AGAATAGCTT GATGTAGTCC TCAGTGAGCC CATATCCTTG
              961 CCTGGATACC CTGGGCCAGA TGGGACCCTC TTTGGCAACC CGCCTTTCCC CCGGACTTCC
             1021 CTTAGAGAGG GGAGCCGCCA CCAGGAAGGC TATTTCCGTG TAAATTAAGA TAATTCGGTA
             1081 TTGGTCATCA AGTGCTTTGC CGAGACGAGA AATAAGGTGG CCACCAGCAA AGCGCGCCAC
            1141 CATG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Niwa, H., Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells. Mol. Cell. Biol., 22, 1526-1536 (2002). PMID 11839818.

            2022.05.17

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