Resource data sheet
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ptk-luc

A control reporter plasmid under tk promoter.

Catalog number RDB07031
Resource name ptk-luc
Alternative name stock no 649
Clone info. A control reporter plasmid under tk promoter.
168-bp BamHI-XhoI fragment derived from pBLCAT2 containing minimal herpes simplex virus thymidine kinase (tk) promoter (Niwa, H., Mol. Cell. Biol., 1526-1536, 2002)..
Vector backbone pGL3-basic (Plasmid)
Depositor Niwa, Hitoshi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Mol. Cell. Biol., 22, 1526-1536 (2002)).
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07031 ptk-luc DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB07031z.seq from Depositor
Electronic file Map RDB07031a.gif from Depositor
Electronic file PDF (map, protocol and/or remarks) RDB07031p.pdf from Depositor

Original reference

original Niwa, H., Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells. Mol. Cell. Biol., 22, 1526-1536 (2002). PMID 11839818.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Control Vectors (English text)
Featured content Reguration of genes in ES cells (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB07031_A9Dcp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pause site RDB07031_A9Dca.seq
>07031_07031_A9Dc_2-A_pause site_A02_01_ABI08.ab1
    1 GTTCCATTTT CTCTATCGAT AGGTACCGAG CTCTTACGCG TGCTAGCCCG GGCTCGAGAT
   61 CCGGCCCCGC CCAGCGTCTT GTCATTGGCG AATTCGAACA CGCAGATGCA GTCGGGGCGG
  121 CGCGGTCCGA GGTCCACTTC GCATATTAAG GTGACGCGTG TGGCCTCGAA CACCGAGCGA
  181 CCCTGCAGCG ACCCGCTTAA CAGCGTCAAC AGCGTGCCGC AGATCTCGAA GCTTGGCATT
  241 CCGGTACTGT TGGTAAAGCC ACCATGGAAG ACGCCAAAAA CATAAAGAAA GGCCCGGCGC
  301 CATTCTATCC GCTGGAAGAT GGAACCGCTG GAGAGCAACT GCATAAGGCT ATGAAGAGAT
  361 ACGCCCTGGT TCCTGGAACA ATTGCTTTTA CAGATGCACA TATCGAGGTG GACATCACTT
  421 ACGCTGAGTA CTTCGAAATG TCCGTTCGGT TGGCAGAAGC TATGAAACGA TATGGGCTGA
  481 ATACAAATCA CAGAATCGTC GTATGCAGTG AAAACTCTCT TCAATTCTTT ATGCCGGTGT
  541 TGGGCGCGTT ATTTATCGGA GTTGCAGTTG CGCCCGCGAA CGACATTTAT AATGAACGTG
  601 AATTGCTCAA CAGTATGGGC ATTTCGCAGC CTACCGTGGT GTTCGTTTCC AAAAAGGGGT
  661 TGCAAAAAAT TTTGAACGTG CAAAAAAAGC TCCCAATCAT CCAAAAAATT ATTATCATGG
  721 ATTCTAAAAC GGATTACCAG GGATTTCAGT CGATGTACAC GTTCGTCACA TCTCATCTAC
  781 CTCCCGGTTT TAATGAATAC GATTTTGTGC CAGAGTCCTT CGATAGGGGA CAAGACAATT
  841 GCACTGATCA TGAACTCCTC TGGATCTACT GGTCTGCCTA AAGGTGTCGC TCTGCCTCAT
  901 AGAACTGCCT GCGTGAGATT CTCGGCATGC CAGAAGATCC TATTTTTTGG GCAATCAAAA
  961 TCATTCCGGG ATACTGGCGA TTTTTAAAGT GTTGGTTCCC ATTTCCAATC ACGGGTTTTT
 1021 GGGAAATGGT TAACTTAACA CTTCCGGGAT ATTTTGGATT ATGTGGGAAT TTCGGAGTTC
 1081 GGTCCTTAAA TGTATAAGAA ATTGGAAGAA GAGCTGGTTT TCTGGAGGAG CCTTCAGGAA
1141 TTACCAAGAG AAATTCCAAA GAGTGTC
//
Primer: pGL3_Rv4 RDB07031_A9Dcb.seq
>07031_07031_A9Dc_2-A_pGL3_Rv4_B02_02_ABI08.ab1
    1 GGAGAAAGGC ATGGGGGTTG AGGCTCTCAA GGGCATCGGT CGACGGATCC TTATCGATTT
   61 TACCACATTT GTAGAGGTTT TACTTGCTTT AAAAAACCTC CCACACCTCC CCCTGAACCT
  121 GAAACATAAA ATGAATGCAA TTGTTGTTGT TAACTTGTTT ATTGCAGCTT ATAATGGTTA
  181 CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG
  241 TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGCTCGAAGC GGCCGGCCGC
  301 CCCGACTCTA GAATTACACG GCGATCTTTC CGCCCTTCTT GGCCTTTATG AGGATCTCTC
  361 TGATTTTTCT TGCGTCGAGT TTTCCGGTAA GACCTTTCGG TACTTCGTCC ACAAACACAA
  421 CTCCTCCGCG CAACTTTTTC GCGGTTGTTA CTTGACTGGC GACGTAATCC ACGATCTCTT
  481 TTTCCGTCAT CGTCTTTCCG TGCTCCAAAA CAACAACGGC GGCGGGAAGT TCACCGGCGT
  541 CATCGTCGGG AAGACCTGCG ACACCTGCGT CGAAGATGTT GGGGTGTTGG AGCAAGATGG
  601 ATTCCAATTC AGCGGGAGCC ACCTGATAGC CTTTGTACTT AATCAGAGAC TTCAGGCGGT
  661 CAACGATGAA GAAGTGTTCG TCTTCGTCCC AGTAAGCTAT GTCTCCAGAA TGTAGCCATC
  721 CATCCTTGTC AATCAAGGCG TTGGTCGCTT CCGGATTGTT TACATAACCG GACATAATCA
  781 TAGGACCTCT CACACACAGT TCGCCTCTTT GATTAACGCC CAGCGTTTTC CCGGTATCCA
  841 GATCCACAAC CTTCGCTTCA AAAAAATGGG AACAACTTTA CCGACCGCGC CCGGGTTTAT
  901 CATCCCCCTC GGGTGTAATC AGAATAGCTT GATGTAGTCC TCAGTGAGCC CATATCCTTG
  961 CCTGGATACC CTGGGCCAGA TGGGACCCTC TTTGGCAACC CGCCTTTCCC CCGGACTTCC
 1021 CTTAGAGAGG GGAGCCGCCA CCAGGAAGGC TATTTCCGTG TAAATTAAGA TAATTCGGTA
 1081 TTGGTCATCA AGTGCTTTGC CGAGACGAGA AATAAGGTGG CCACCAGCAA AGCGCGCCAC
1141 CATG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Niwa, H., Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells. Mol. Cell. Biol., 22, 1526-1536 (2002). PMID 11839818.

2019.05.13

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