Please review the QC test results indicated by 
icon below as well as clone information before placing your order.
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Please review the QC test results indicated by icon below as well as clone information before placing your order.
icon below as well as clone information before placing your order.
pCMFlag_hsINS (#RDB07024)
Expression vector of human INS.
| Alternative name | SET-0203_12 |
|---|---|
| Clone info. | Expression vector of human INS. Expression has not yet been confirmed. |
| Comment | Derived from Sugano PNC01554. PCR cloning, forward primer: gccctgtggatgcgcctcctgcc; reverse primer: ctagttgcagtagttctccagc. |
| Vector backbone | pCMV_S-FLAG (RDB05956) (Plasmid) |
| Size of vector backbone | 5.5 kb |
| Selectable markers | Amp^r (E. coli), Neo^r (mammalian cell) |
| Gene/insert name | Human INS cDNA |
| Depositor|Developer | DNA Bank, | |
Distribution information
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Ordering forms | Order form [Credit Card Payment  ] [Bank Transfer Payment] [Example of order form ]MTA, for use for not-for-profit academic purpose [Word ] [Example of MTA ]Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
|---|---|
| Terms and conditions for distribution | The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL and cite Gene 200, 149-156, 1997 in any publication. |
[open/close]| 必要書類 | 提供依頼書 [依頼書の記入例 ]提供同意書 (MTA, 非営利学術目的用)[Word ] [MTAの記入例 ]手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
|---|---|
| MTAに書く使用条件 | 発表に際し、理研BRCから入手したことを明記し、指定する文献(Gene, 200, 149-156 (1997))を引用すること。 |
| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB07024 | pCMFlag_hsINS | DNA solution |
icon below as well as clone information before placing your order.How to cite this biological resource
Materials & Methods section:
| The pCMFlag_hsINS was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07024). |
Reference section:
Further references such as user reports and related articles (go to bottom)
QC test results
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB12835_A4Gvp1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: CMV-Forward Sequence file: RDB12835_A4Gva.seq
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>07024_12835_A4Gv_CMV-Foward_C02_08.ab1 1 CAGATTAAAA ACCCCCCGGC CCCCATTGAC GCAAATGGAC GGTAGGCGTG TACGGTGGGA 61 GGTCTATATA AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA 121 AATTAATACG ACTCACTATA GGGAGACCCA AGCTGAATTC ACCATGGACT ACAAGGACGA 181 TGACGATAAG GCGGCCGCCC TGTGGATGCG CCTCCTGCCC CTGCTGGCGC TGCTGGCCCT 241 CTGGGGACCT GACCCAGCCG CAGCCTTTGT GAACCAACAC CTGTGCGGCT CACACCTGGT 301 GGAAGCTCTC TACCTAGTGT GCGGGGAACG AGGCTTCTTC TACACACCCA AGACCCGCCG 361 GGAGGCAGAG GACCTGCAGG TGGGGCAGGT GGAGCTGGGC GGGGGCCCTG GTGCAGGCAG 421 CCTGCAGCCC TTGGCCCTGG AGGGGTCCCT GCAGAAGCGT GGCATTGTGG AACAATGCTG 481 TACCAGCATC TGCTCCCTCT ACCAGCTGGA GAACTACTGC AACTAGGGCC GCTCGAGCAT 541 GCATCTAGAG GGCCCTATTC TATAGTGTCA CCTAAATGCT AGAGCTCGCT GATCAGCCTC 601 GACTGTGCCT TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC 661 CCTGGAAGGT GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG CATCGCATTG 721 TCTGAGTAGG TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CAGGACAGCA AGGGGGAGGA 781 TTGGGAAGAC AATAGCAGGC ATGCTGGGGG ATGCGGTGGG CTCTATGGGC TTCTGAGGCG 841 AAAAGAACCA GCTGGGGGGC TCGAGGGGGG ATCCCCCACG CGCCCTGTAG CGGCGCATTA 901 AGCGCGGGCG GGGTGTGGGT GG // |
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Primer: SV40pro_F_V2 Sequence file: RDB12835_A4Gvb.seq
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>07024_12835_A4Gv_SV40pro_F_V2_D04_10.ab1 1 GTAGGAGGTT TTTTGGGAGG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGGATATC 61 CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG 121 ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA 181 ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT 241 TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG 301 GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA 361 AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA 421 CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT 481 TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC 541 TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC 601 GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT 661 GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA AAATGGCCGC TTTTCTGGAT 721 TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA GGACATAGCG TTGGCTACCC 781 GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGGCTGACC GCTTCCTCGT GCTTTACGGT 841 ATCGCCGCTC CCGATTCGCA GCGCATCGCC CTTCTATCGC CTTCTTGACG AGTTCTTCTG 901 AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC CCAACCTGCC CATCACGAGA 961 TTTTCGATTT CCACCCGCCG CCCTTCCTAT GAAAGGTTGG GCTTTCGGAA ATCGGTTTTC 1021 GGACGTCCGG CCTGGAATGA TTCTCAGCGC GGGGATCTCA TGCTGCAAGC TTCCTTCGAC 1081 CTAGCTCCGA ACCTTGTTA // |
Please visit 
Sequencing and PCR primers for primer information.
References
Original, user report and related articles
Resource search
Link
Other resources in our bankExternal Database
Human INS
Reference sequence
Tips
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