Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCMFlag_hsE2F4 (#RDB06786)

Expression vector of human E2F4.

Alternative name	SET-0113_9-1
Clone info.	Expression vector of human E2F4. Expression has not yet been confirmed.
Comment	PCR cloning, forward primer: gcggaggccgggccacaggc; reverse primer: tcagaggttgagaacaggcacatc.
Vector backbone	pCMV_S-FLAG (RDB05956) (Plasmid)
Size of vector backbone	5.5 kb
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cell)
Growth conditions	37C, LB+Amp
Gene/insert name	Human E2F transcription factor 4,p107/p103-binding cDNA
Depositor|Developer	DNA Bank, |

 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution

The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL in any publication. Additional terms and conditions: Regrding pRc/CMV: a) The modified pRc/CMV, and their descendants (collectively, LIFE MATERIALS), should be used for pre-clinical/non-human, research use only. b) LIFE MATERIALS shall not be used for any commercial purposes. c) LIFE MATERIALS shall not be transferred beyond the specified recipient. d) Recipient must contact Thermo Fisher Scientific Inc. at outlicense@thermofisher.com for any desired use of LIFE MATERIALS beyond the scope of a) to c) above.

提供案内 (日本国内) [open/close]

Catalog #	Resource name	Shipping form	Fee
RDB06786	pCMFlag_hsE2F4	DNA solution	JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The pCMFlag_hsE2F4 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06786).

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12837_A4Hb.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB12837_A4Hba.seq
>06786_12837_A4Hb_CMV-Foward_C07_07.ab1     1 CGTTCCTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG    61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC   121 TCACTATAGG GAGACCCAAG CTGAATTCAC CATGGACTAC AAGGACGATG ACGATAAGGC   181 GGCCGCGGAG GCCGGGCCAC AGGCGCCGCC GCCCCCGGGC ACTCCAAGCC GGCACGAAAA   241 GAGCCTGGGA CTGCTCACCA CCAAGTTCGT GTCCCTTCTG CAGGAGGCCA AGGACGGCGT   301 GCTTGACCTC AAGCTGGCAG CTGACACCCT AGCTGTACGC CAGAAGCGGC GGATTTACGA   361 CATTACCAAT GTTTTGGAAG GTATCGGGCT AATCGAGAAA AAGTCCAAGA ACAGCATCCA   421 GTGGAAGGGT GTGGGGCCTG GCTGCAATAC CCGGGAGATT GCTGACAAAC TGATTGAGCT   481 CAAGGCAGAG ATCGAGGAGC TGCAGCAGCG GGAGCAAGAA CTAGACCAGC ACAAGGTGTG   541 GGTGCAGCAG AGCATCCGGA ACGTCACAGA GGACGTGCAG AACAGCTGTT TGGCCTACGT   601 CACTCATGAG GACATCTGCA GATGCTTTGC TGGAGATACC CTCTTGGCCA TCCGGGCCCC   661 ATCAGGCACC AGCCTGGAGG TGCCCATCCC AGAGGGTCTC AATGGGCAGA AGAAGTACCA   721 GATTCACCTG AAGAGTGTGA GTGGTCCCAT TGAGGTTCTG CTGGTGAACA AGGAGGCATG   781 GAGCTCACCC CCTGTGGCTG TGCCTGTGCC ACCACCTGAA GATTTGCTCC AGAGCCCATC   841 TGCTGTTTCT ACACCTCCAC CTCTGCCCAA GCCTGCCCTA GCCCAGTCCC AGGAAGCCTC   901 ACGTCCAAAT AGTCCTCAGC TCACTCCCAC TGCTGTCCCT GGCAGTGCAG AAGTCCAGGG   961 AATGGCTGGG CCCAGCAGCT GAGATCACAG TGAGTGGCGG GCCCTGGGAC TGATAGCAAA  1021 GGGACAGTGG TGAGCTCAGT TCACTCCCCA CTGGGCCCCA ACACACTGGA CACCCGGGCA  1081 CTGCAGTCTT CTGCCTTGCT TGGACAGCCA GCCAGCCAGG CAGCAAGGCA GGCCAGGCCA 1141 GG //
Primer: BGH_rev Sequence file: RDB12837_A4Hbb.seq
>06786_12837_A4Hb_BGHrev_D07_10.ab1     1 GCCTTACCCA TTAGGGTGAA CTATAGAATA GGGCCCTCTA GATGCATGCT CGAGCGGCTC    61 AGAGGTTGAG AACAGGCACA TCAAAGAGGT CACAGACACC TTCACTCTCG TCCAGGTTGT   121 AGATATAATC GTGGTCTCCC GGGGGTGGAG AAAGACGAAG CAGAGGGGCA AACACTTCTG   181 AGGACATCAA CTCCTCCAGC AGCTCCGAGC TCATGCACTC TCGTGTGGGA TCAAAGATTT   241 CTGACAGCTC TTTGGGGAGT TCCAAAACAC CTGTGGGGTC TGCCTTGATG GGCTCAAAGG   301 AGGTAGAAGG GTTGGGTCCG GACGAACTGC TGCTGTTACT GTTGCTGCTG CTGCTGCTGC   361 TGCTGCTGCT GCTGCTGCTG CTGTCCAGCA GGGCAGAAGA CTGCAGTGGC CGGGTGTCCA   421 GTGTTGTTGG GCCCAGTGGG AGTGAACTGA GCTCACCACT GTCCTTGCTA TCAGTCCCAG   481 GGCCGCCACT CACTGTGATC TCAGCTGCTG GGCCAGCCAT TCCCTGGACT TCTGCACTGC   541 CAGGGACAGC AGTGGGAGTG AGCTGAGGAC TATTTGGACG TGAGGCTTCC TGGGACTGGG   601 CTAGGGCAGG CTTGGGCAGA GGTGGAGGTG TAGAAACAGC AGATGGGCTC TGGAGCAAAT   661 CTTCAGGTGG TGGCACAGGC ACAGCCACAG GGGGTGAGCT CCATGCCTCC TTGTTCACCA   721 GCAGAACCTC AATGGGACCA CTCACACTCT TCAGGTGAAT CTGGTACTTC TTCTGCCCAT   781 TGAGACCCTC TGGGATGGGC ACCTCCAGGC TGGTGCCTGA TGGGGCCCGG ATGGCCAAGA   841 GGGTATCTCC AGCAAAGCAT CTGCAGATGT CCTCATGAGT GACGTAGGCC AAACAGCTGT   901 TCTGCACGTC CTCTGTGACG TTCCGGATGC TCTGCTGCAC CCACACCTTG TGCTGGTCTA   961 GTTCTTGCTC CCGCTGCTGC AGCTCCTCGA TCTCTGCCTT GAGCTCAATC AGTTGTCAGC  1021 AAATCTCCCG GGTATTGCAG CCCAGGGCCC CACACCCTTT CACTGGAATG CTGGTTCTTG  1081 GACTTTTTTC TCGATTAGCC CGGATTACCC TCCAAACATG GTTAATGTTC GTAAATCCCG 1141 TCCGCGCTTC CTTGC //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

Resource search

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Other resources in our bank

• human E2F transcription factor 4,p107/p103-binding

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External Database
Human E2F transcription factor 4,p107/p103-binding

• in KEGG Gene
• in NCBI Gene

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Reference sequence
  

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