Sequencing and PCR primers for primer information.
Reporter construct containing the hexamerized 50 bp EBNA2 response element of the TP-1 promoter. TP1-luciferase reporter.
| Catalog number | RDB06776 |
|---|---|
| Resource name | pGa981-6 |
| Alternative name | TP1-luc(981-6) , #9507 |
| Clone info. | Reporter construct containing the hexamerized 50 bp EBNA2 response element of the TP-1 promoter. The response element was inserted in front of the mnimal beta-globin promoter of pGa50-7. Please refer Fig.1 of Oswald, F., Mol. Cell. Biol. 21 (22): 7761-7774 (2001). |
| Vector backbone | pGa50-7 (Plasmid) |
| Selectable markers | Amp^r |
| Gene/insert name | Artifical EBNA2 response element of the TP-1 synthesized |
| Depositor | Honjo, Tasuku | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Nucleic Acids Res., 26, 5448-5455 (1998).). The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@phy.med.kyoto-u.ac.jp). When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources. |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB06776 | pGa981-6 | DNA solution |
Electronic file
| Electronic file | PDF RDB06776.pdf from Depositor |
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Featured content
| Featured content | Dnaconda's recommendation EXR0030j (Japanese text) |
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| Featured content | Promoter Collection (Outline) (English text) |
| Featured content | Promoter Collection (Outline) (Japanese text) |
| Featured content | Notch signaling pathway (English text) |
References
User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB14402_A6Kmp1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: Amp_R | RDB14402_A6Kma.seq |
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>06776_14402_A6Km_Amp_R_A10_01_ABI24.ab1 1 TTTCGTTTTA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 121 CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG 181 AGGCCTATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGC 241 CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA 301 TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG 361 TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTGCCA AGCTTGCATG CCTGCAGGTC 421 GACTCTAGAG GATCCCGACT CATGGGAAAA TGGGCGGAAG GGCACCGTGG GAAAATAGTA 481 GATCCCGACT CGTGGGAAAA TGGGCGGAAG GGCACCGTGG GAAAATAGTA GATCCCGACT 541 CGTGGGAAAA TGGGCGGAAG GGCACCGTGG GAAAATAGTA GATCCCGACT CATGGGAAAA 601 TGGGCGGAAG GGCACCGTGG GAAAATAGTA GATCCCGACT CGTGGGAAAA TGGGCGGAAG 661 GGCACCGTGG GAAAATAGTA GATCCCGACT CGTGGGAAAA TGGGCGGAAG GGCACCGTGG 721 GAAAATAGTA GATCCACTGT ATGCATCTTG GGCATAAAAG GCAGATCACT TCAGCTTCTG 781 CTTACACTTG CTTTTGACAC AACTGTGTTT ACTTGCAATC CCTCGAGGGA GCTTGGCATT 841 CCGGTACTGT TGGTAAAATG GAAGACGCCA AAAACATAAA GAAAGGCCCG GCGCCATTCT 901 ATCCTCTAGA GGATGGAACC GCTGGAGAGC AACTGCATAA GCTATGAAGA GATACGCCCC 961 TGGTTCCTGG AACAATTGCT TTTACAGATG CACATATCGA GGGTGACATC ACGTACGCGG 1021 ATACTTCGAA TGTCCGTTCA TGCAGAGCTA TGAAACGAAT ATGGGGCTGA TACAATCCAC 1081 AGAATCGTCG TATGCCATGA ACTCTCTTCC ATTCCTTTAT TGCCGGTGTT TGGGGGGCGC 1141 GGCG // |
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| Primer: SV40-3'UTR | RDB14402_A6Kmb.seq |
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>06776_14402_A6Km_SV40-3'UTR_D03_12_ABI24.ab1 1 TACAGTAGTA CGTTCCTTCA CAAAGATCCT CTAGAGTATT ACAATAGCTA AGAATTTCGT 61 CATCGCTGAA TACAGTTACA TTTTACAATT TGGACTTTCC GCCCTTCTTG GCCTTTATGA 121 GGATCTCTCT GATTTTTCTT GCGTCGAGTT TTCCGGTAAG ACCTTTCGGT ACTTCGTCCA 181 CAAACACAAC TCCTCCGCGC AACTTTTTCG CGGTTGTTAC TTGACTGGCG ACGTAATCCA 241 CGATCTCTTT TTCCGTCATC GTCTTTCCGT GCTCCAAAAC AACAACGGCG GCGGGAAGTT 301 CACCGGCGTC ATCGTCGGGA AGACCTGCCA CGCCCGCGTC GAAGATGTTG GGGTGTTGTA 361 ACAATATCGA TTCCAATTCA GCGGGGGCCA CCTGATATCC TTTGTATTTA ATTAAAGACT 421 TCAAGCGGTC AACTATGAAG AAGTGTTCGT CTTCGTCCCA GTAAGCTATG TCTCCAGAAT 481 GTAGCCATCC ATCCTTGTCA ATCAAGGCGT TGGTCGCTTC CGGATTGTTT ACATAACCGG 541 ACATAATCAT AGGTCCTCTG ACACATAATT CGCCTCTCTG ATTAACGCCC AGCGTTTTCC 601 CGGTATCCAG ATCCACAACC TTCGCTTCAA AAAATGGAAC AACTTTACCG ACCGCGCCCG 661 GTTTATCATC CCCCTCGGGT GTAATCAGAA TAGCTGATGT AGTCTCAGTG AGCCCATATC 721 CTTGTCGTAT CCCTGGAAGA TGGAAGCGTT TTGCAACCGC TTCCCCGACT TCTTTCGAAA 781 GAGGTGCGCC CCCAGAAGCA ATTTCGTGTA AATTAGATAA ATCGTATTTG TCAATCAGAG 841 TGCTTTTGGC GAAGAATGAA AATAGGGTTG GTACTAGCAA CGCACTTTGA ATTTTGTAAT 901 CCTGAAAGGG ATCGTAAAAA CAGCTCTTCT TCAAATCTAT ACATTAAGAC GACTCGAAAT 961 CCACATATCA AATATCCGAG TGTAGTAAAC ATTCCCAAAA CCCGTGATGG AATGGAACAA 1021 CACTTAAAAT CGCAGTATCC CGGGAATGAT TTGATTGCCA AAAAATAGGA TCTCTGGGCA 1081 TGCGAGATCT GGACGGCAGC AGTCTATGCC GACGCCACAC CCTGTAGTTA CCTCAGTAGA 1141 AACTTCCAGA GGAGGGAATG A // |
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Please visit Sequencing and PCR primers for primer information.
References
2018.12.11
GNP_filter3_RDBDEP_html_181012.pl