RDB06776 - pGa981-6

Resource data sheet
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pGa981-6

Reporter construct containing the hexamerized 50 bp EBNA2 response element of the TP-1 promoter. TP1-luciferase reporter.

Catalog number	RDB06776
Resource name	pGa981-6
Alternative name	TP1-luc(981-6) , #9507
Clone info.	Reporter construct containing the hexamerized 50 bp EBNA2 response element of the TP-1 promoter. The response element was inserted in front of the mnimal beta-globin promoter of pGa50-7. Please refer Fig.1 of Oswald, F., Mol. Cell. Biol. 21 (22): 7761-7774 (2001).
Vector backbone	pGa50-7 (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Artifical EBNA2 response element of the TP-1 synthesized
Depositor	Honjo, Tasuku \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Nucleic Acids Res., 26, 5448-5455 (1998).). The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.

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Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Nucleic Acids Res., 26, 5448-5455 (1998).). The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.
Ordering	Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Nucleic Acids Res., 26, 5448-5455 (1998).)。公表を前提とした学術研究に限る。営利機関の利用者は、事前に京都大学・「医学領域」産学連携推進機構・知財室 (contracts@kumbl.med.kyoto-u.ac.jp) に連絡し、承諾を得ること。理研BRCは、本件リソースのリソース名、提供先、利用目的、件数を京都大学・本庶佑教授に報告する。
提供依頼	手続きの詳細は、「提供申込みについて」をご覧ください。提供依頼書 [Word] 提供同意書 [Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB06776	pGa981-6	DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file	PDF (map, protocol and/or remarks) RDB06776.pdf from Depositor

Original reference

original	Kurooka, H., Roles of the ankyrin repeats and C-terminal region of the mouse notch1 intracellular region. Nucleic Acids Res., 6(23): 5448-5455 (1998). PMID 9826771.
original	Minoguchi, S., RBP-L, a transcription factor related to RBP-Jkappa. Mol. Cell. Biol., 17, 2679-2687 (1997). PMID 9111338.

Further references such as user reports and related articles (go to bottom)

Featured content	Dnaconda's recommendation EXR0030j (Japanese text)
Featured content	Promoter Collection (Outline) (English text)
Featured content	Promoter Collection (Outline) (Japanese text)
Featured content	Notch signaling pathway (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet	RDB14402_A6Kmp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Amp_R	RDB14402_A6Kma.seq
>06776_14402_A6Km_Amp_R_A10_01_ABI24.ab1 1 TTTCGTTTTA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 121 CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG 181 AGGCCTATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGC 241 CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA 301 TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG 361 TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTGCCA AGCTTGCATG CCTGCAGGTC 421 GACTCTAGAG GATCCCGACT CATGGGAAAA TGGGCGGAAG GGCACCGTGG GAAAATAGTA 481 GATCCCGACT CGTGGGAAAA TGGGCGGAAG GGCACCGTGG GAAAATAGTA GATCCCGACT 541 CGTGGGAAAA TGGGCGGAAG GGCACCGTGG GAAAATAGTA GATCCCGACT CATGGGAAAA 601 TGGGCGGAAG GGCACCGTGG GAAAATAGTA GATCCCGACT CGTGGGAAAA TGGGCGGAAG 661 GGCACCGTGG GAAAATAGTA GATCCCGACT CGTGGGAAAA TGGGCGGAAG GGCACCGTGG 721 GAAAATAGTA GATCCACTGT ATGCATCTTG GGCATAAAAG GCAGATCACT TCAGCTTCTG 781 CTTACACTTG CTTTTGACAC AACTGTGTTT ACTTGCAATC CCTCGAGGGA GCTTGGCATT 841 CCGGTACTGT TGGTAAAATG GAAGACGCCA AAAACATAAA GAAAGGCCCG GCGCCATTCT 901 ATCCTCTAGA GGATGGAACC GCTGGAGAGC AACTGCATAA GCTATGAAGA GATACGCCCC 961 TGGTTCCTGG AACAATTGCT TTTACAGATG CACATATCGA GGGTGACATC ACGTACGCGG 1021 ATACTTCGAA TGTCCGTTCA TGCAGAGCTA TGAAACGAAT ATGGGGCTGA TACAATCCAC 1081 AGAATCGTCG TATGCCATGA ACTCTCTTCC ATTCCTTTAT TGCCGGTGTT TGGGGGGCGC 1141 GGCG //
Primer: SV40-3'UTR	RDB14402_A6Kmb.seq
>06776_14402_A6Km_SV40-3'UTR_D03_12_ABI24.ab1 1 TACAGTAGTA CGTTCCTTCA CAAAGATCCT CTAGAGTATT ACAATAGCTA AGAATTTCGT 61 CATCGCTGAA TACAGTTACA TTTTACAATT TGGACTTTCC GCCCTTCTTG GCCTTTATGA 121 GGATCTCTCT GATTTTTCTT GCGTCGAGTT TTCCGGTAAG ACCTTTCGGT ACTTCGTCCA 181 CAAACACAAC TCCTCCGCGC AACTTTTTCG CGGTTGTTAC TTGACTGGCG ACGTAATCCA 241 CGATCTCTTT TTCCGTCATC GTCTTTCCGT GCTCCAAAAC AACAACGGCG GCGGGAAGTT 301 CACCGGCGTC ATCGTCGGGA AGACCTGCCA CGCCCGCGTC GAAGATGTTG GGGTGTTGTA 361 ACAATATCGA TTCCAATTCA GCGGGGGCCA CCTGATATCC TTTGTATTTA ATTAAAGACT 421 TCAAGCGGTC AACTATGAAG AAGTGTTCGT CTTCGTCCCA GTAAGCTATG TCTCCAGAAT 481 GTAGCCATCC ATCCTTGTCA ATCAAGGCGT TGGTCGCTTC CGGATTGTTT ACATAACCGG 541 ACATAATCAT AGGTCCTCTG ACACATAATT CGCCTCTCTG ATTAACGCCC AGCGTTTTCC 601 CGGTATCCAG ATCCACAACC TTCGCTTCAA AAAATGGAAC AACTTTACCG ACCGCGCCCG 661 GTTTATCATC CCCCTCGGGT GTAATCAGAA TAGCTGATGT AGTCTCAGTG AGCCCATATC 721 CTTGTCGTAT CCCTGGAAGA TGGAAGCGTT TTGCAACCGC TTCCCCGACT TCTTTCGAAA 781 GAGGTGCGCC CCCAGAAGCA ATTTCGTGTA AATTAGATAA ATCGTATTTG TCAATCAGAG 841 TGCTTTTGGC GAAGAATGAA AATAGGGTTG GTACTAGCAA CGCACTTTGA ATTTTGTAAT 901 CCTGAAAGGG ATCGTAAAAA CAGCTCTTCT TCAAATCTAT ACATTAAGAC GACTCGAAAT 961 CCACATATCA AATATCCGAG TGTAGTAAAC ATTCCCAAAA CCCGTGATGG AATGGAACAA 1021 CACTTAAAAT CGCAGTATCC CGGGAATGAT TTGATTGCCA AAAAATAGGA TCTCTGGGCA 1081 TGCGAGATCT GGACGGCAGC AGTCTATGCC GACGCCACAC CCTGTAGTTA CCTCAGTAGA 1141 AACTTCCAGA GGAGGGAATG A //
Primer: Luc-M_R	RDB14402_A6Kmc.seq
>06776_14402_A9FiF_Luc-M_R_A05_02_ABI24.ab1 1 CATCCGGACT TCTTTCGAAG AGGTGCGCCC CCAGAAGCAA TTTCGTGTAA ATTAGATAAA 61 TCGTATTTGT CAATCAGAGT GCTTTTGGCG AAGAATGAAA ATAGGGTTGG TACTAGCAAC 121 GCACTTTGAA TTTTGTAATC CTGAAGGGAT CGTAAAAACA GCTCTTCTTC AAATCTATAC 181 ATTAAGACGA CTCGAAATCC ACATATCAAA TATCCGAGTG TAGTAAACAT TCCAAAACCG 241 TGATGGAATG GAACAACACT TAAAATCGCA GTATCCGGAA TGATTTGATT GCCAAAAATA 301 GGATCTCTGG CATGCGAGAA TCTGACGCAG GCAGTTCTAT GCGGAAGGGC CACACCCTTA 361 GGTAACCCAG TAGATCCAGA GGAATTCATT ATCAGTGCAA TTGTTTTGTC ACGATCAAAG 421 GACTCTGGTA CAAAATCGTA TTCATTAAAA CCGGGAGGTA GATGAGATGT GACGAACGTG 481 TACATCGACT GAAATCCCTG GTAATCCGTT TTAGAATCCA TGATAATAAT TTTCTGGATT 541 ATTGGTAATT TTTTTTGCAC GTTCAAAATT TTTTGCAACC CCTTTTTGGA AACAAACACT 601 ACGGTAGGCT GCGAAATGTT CATACTGTTG AGCAATTCAC GTTCATTATA AATGTCGTTC 661 GCGGGCGCAA CTGCAACTCC GATAAATAAC GCGCCCAACA CCGGCATAAA GAATTGAAGA 721 GAGTTTTCAC TGCATACGAC GATTCTGTGA TTTGTATTCA GCCCATATCG TTTCATAGCT 781 TCTGCCAACC GAACGGACAT TTCGAAGTAT TCCGCGTACG TGATGTTCAC CTCGATATGT 841 GCATCTGTAA AAGCAATTGT TCCAGGAACC AGGGCGTATC TCTTCATAGC CTTATGCAGT 901 TGCTCTCCAG CGGTTCCATC CTCTAGAGGG ATAGAATGGC GCCCGGGCCT TTCTTTATGT 961 TTTTGGGCGT CTTCCATTTT ACCAACAGTA CCGGAATGCC AAGCTTCCCT CGAGGGATTG 1021 CAGTAAACAC AGGTTGTGTC AAAAGCAAGT GTAAGCAGAG CTGAGGTGAT CTGCCTTTTT 1081 ATGCCCAGAT GCATTACCAG TGGAATCTAC CTATTTTTCC CCAACGGGGG TGCCCCCCAT //
Primer: SV40polyA	RDB14402_A6Kmd.seq
>06776_14402_A9FiF_SV40polyA_R2_B05_05_ABI24.ab1 1 CTTAATAGGC CTGCAATAAC AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT 61 TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC 121 TGATTATGAT CTCTAGTCAA GGCACTATAC ATCAAATATT CCTTATTAAC CCCTTTACAA 181 ATTAAAAAGC TAAAGGTACA CAATTTTTGA GCATAGTTAT TAATAGCAGA CACTCTATGC 241 CTGTGTGGAG TAAGAAAAAA CAGTATGTTA TGATTATAAC TGTTATGCCT ACTTATAAAG 301 GTTACAGAAT ATTTTTCCAT AATTTTCTTG TATAGCAGTG CAGCTTTTTC CTTTGTGGTG 361 TAAATAGCAA AGCAAGCAAG AGTTCTATTA CTAAACACAG CATGACTCAA AAAACTTAGC 421 AATTCTGAAG GAAAGTCCTT GGGGTCTTCT ACCTTTCTCT TCTTTTTTGG AGGAGTAGAA 481 TGTTGAGAGT CAGCAGTAGC CTCATCATCA CTAGATGGCA TTTCTTCTGA GCAAAACAGG 541 TTTTCCTCAT TAAAGGCATT CCACCACTGC TCCCATTCAT CAGTTCCATA GGTTGGAATC 601 TAAAATACAC AAACAATTAG AATCAGTAGT TTAACACATT ATACACTTAA AAATTTTATA 661 TTTACCTTAG AGCTTTAAAT CTCTGTAGGT AGTTTGTCCA ATTATGTCAC ACCACAGAAG 721 TAAGGTTCCT TCACAAAGAT CCTCTAGAGT ATTACAATAG CTAAGAATTT CGTCATCGCT 781 GAATACAGTT ACATTTTACA ATTTGGACTT TCCGCCCTTC TTGGCCTTTA TGAGGATCTC 841 TCTGATTTTT CTTGCGTCGA GTTTTCCGGT AAGACCTTTC GGTACTTCGT TCCACAAACA 901 CAACTCCTCC GCGCAACTTT TTTCGCGGTT GTTACTTGAC TGGCGACGTA ATCCACGATC 961 TCTTTTTCCG TCATCGTCTT TCCGTGCTCC AAAACAACAA CGGCGGCGGG AAGTTCACCG 1021 GGCGTCATCG TCGGGAAGAC CTGGCACGCC CCGCGTCGAA GATGTGGGTG TGGTTACAAA 1081 TATCGGATTT CCAAATTCAG CGGGGCCAAC TTGATATCTT TTGGTAATTA AATTAAAAGG 1141 AACCTTTCA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Kurooka, H., Roles of the ankyrin repeats and C-terminal region of the mouse notch1 intracellular region. Nucleic Acids Res., 6(23): 5448-5455 (1998). PMID 9826771.
original	Minoguchi, S., RBP-L, a transcription factor related to RBP-Jkappa. Mol. Cell. Biol., 17, 2679-2687 (1997). PMID 9111338.
reference	Itatani, Y., Characterization of Aes nuclear foci in colorectal cancer cells. J. Biochem. 159 (1): 133-140 (2016). PMID 26229111.
reference	Shimizu, K., Integrity of intracellular domain of Notch ligand is indispensable for cleavage required for release of the Notch2 intracellular domain. EMBO J. 21 (3): 294-302 (2002). PMID 11823422.
reference	Oswald, F., p300 acts as a transcriptional coactivator for mammalian Notch-1. Mol. Cell. Biol. 21 (22): 7761-7774 (2001). PMID 11604511.
user report	Kawamoto, A., Ubiquitin D is up-regulated by synergy of Notch signalling and TNF-alpha in the inflamed intestinal epithelia of IBD patients. J Crohns Colitis. 2018 Nov 3. doi: 10.1093/ecco-jcc/jjy180. [Epub ahead of print] PMID 30395194.
user report	Yoneyama, T., Notch Inhibitors from Calotropis gigantea That Induce Neuronal Differentiation of Neural Stem Cells. J. Nat. Prod. 80 (9): 2453-2461 (2017). PMID 28817274.
user report	Sugiyama, M., Paired related homeobox 1 is associated with the invasive properties of glioblastoma cells. Oncol. Rep. 33 (3): 1123-1130 (2015). PMID 25522823.
user report	Matsuda, M., Synthetic lateral inhibition governs cell-type bifurcation with robust ratios. Nat. Commun. 6: 6195 (2015). PMID 25652697.
user report	O'Brien, L.L., Wt1a, Foxc1a, and the Notch mediator Rbpj physically interact and regulate the formation of podocytes in zebrafish. Dev. Biol., 358 (2): 318-330 (2011). PMID 21871448.

2019.06.24

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