Resource data sheet
Prev.

pGa981-6

Reporter construct containing the hexamerized 50 bp EBNA2 response element of the TP-1 promoter. TP1-luciferase reporter.

Catalog number RDB06776
Resource name pGa981-6
Alternative name TP1-luc(981-6) , #9507
Clone info. Reporter construct containing the hexamerized 50 bp EBNA2 response element of the TP-1 promoter.
The response element was inserted in front of the mnimal beta-globin promoter of pGa50-7. Please refer Fig.1 of Oswald, F., Mol. Cell. Biol. 21 (22): 7761-7774 (2001).
Vector backbone pGa50-7 (Plasmid)
Selectable markers Amp^r
Gene/insert name Artifical EBNA2 response element of the TP-1 synthesized
Depositor Honjo, Tasuku |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Nucleic Acids Res., 26, 5448-5455 (1998).). The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06776 pGa981-6 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file PDF (map, protocol and/or remarks) RDB06776.pdf from Depositor

Original reference

original Kurooka, H., Roles of the ankyrin repeats and C-terminal region of the mouse notch1 intracellular region. Nucleic Acids Res., 6(23): 5448-5455 (1998). PMID 9826771.
original Minoguchi, S., RBP-L, a transcription factor related to RBP-Jkappa. Mol. Cell. Biol., 17, 2679-2687 (1997). PMID 9111338.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dnaconda's recommendation EXR0030j (Japanese text)
Featured content Promoter Collection (Outline) (English text)
Featured content Promoter Collection (Outline) (Japanese text)
Featured content Notch signaling pathway (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14402_A6Kmp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Amp_R RDB14402_A6Kma.seq
>06776_14402_A6Km_Amp_R_A10_01_ABI24.ab1
    1 TTTCGTTTTA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
   61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
  121 CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG
  181 AGGCCTATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGC
  241 CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA
  301 TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG
  361 TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTGCCA AGCTTGCATG CCTGCAGGTC
  421 GACTCTAGAG GATCCCGACT CATGGGAAAA TGGGCGGAAG GGCACCGTGG GAAAATAGTA
  481 GATCCCGACT CGTGGGAAAA TGGGCGGAAG GGCACCGTGG GAAAATAGTA GATCCCGACT
  541 CGTGGGAAAA TGGGCGGAAG GGCACCGTGG GAAAATAGTA GATCCCGACT CATGGGAAAA
  601 TGGGCGGAAG GGCACCGTGG GAAAATAGTA GATCCCGACT CGTGGGAAAA TGGGCGGAAG
  661 GGCACCGTGG GAAAATAGTA GATCCCGACT CGTGGGAAAA TGGGCGGAAG GGCACCGTGG
  721 GAAAATAGTA GATCCACTGT ATGCATCTTG GGCATAAAAG GCAGATCACT TCAGCTTCTG
  781 CTTACACTTG CTTTTGACAC AACTGTGTTT ACTTGCAATC CCTCGAGGGA GCTTGGCATT
  841 CCGGTACTGT TGGTAAAATG GAAGACGCCA AAAACATAAA GAAAGGCCCG GCGCCATTCT
  901 ATCCTCTAGA GGATGGAACC GCTGGAGAGC AACTGCATAA GCTATGAAGA GATACGCCCC
  961 TGGTTCCTGG AACAATTGCT TTTACAGATG CACATATCGA GGGTGACATC ACGTACGCGG
 1021 ATACTTCGAA TGTCCGTTCA TGCAGAGCTA TGAAACGAAT ATGGGGCTGA TACAATCCAC
 1081 AGAATCGTCG TATGCCATGA ACTCTCTTCC ATTCCTTTAT TGCCGGTGTT TGGGGGGCGC
1141 GGCG
//
Primer: SV40-3'UTR RDB14402_A6Kmb.seq
>06776_14402_A6Km_SV40-3'UTR_D03_12_ABI24.ab1
    1 TACAGTAGTA CGTTCCTTCA CAAAGATCCT CTAGAGTATT ACAATAGCTA AGAATTTCGT
   61 CATCGCTGAA TACAGTTACA TTTTACAATT TGGACTTTCC GCCCTTCTTG GCCTTTATGA
  121 GGATCTCTCT GATTTTTCTT GCGTCGAGTT TTCCGGTAAG ACCTTTCGGT ACTTCGTCCA
  181 CAAACACAAC TCCTCCGCGC AACTTTTTCG CGGTTGTTAC TTGACTGGCG ACGTAATCCA
  241 CGATCTCTTT TTCCGTCATC GTCTTTCCGT GCTCCAAAAC AACAACGGCG GCGGGAAGTT
  301 CACCGGCGTC ATCGTCGGGA AGACCTGCCA CGCCCGCGTC GAAGATGTTG GGGTGTTGTA
  361 ACAATATCGA TTCCAATTCA GCGGGGGCCA CCTGATATCC TTTGTATTTA ATTAAAGACT
  421 TCAAGCGGTC AACTATGAAG AAGTGTTCGT CTTCGTCCCA GTAAGCTATG TCTCCAGAAT
  481 GTAGCCATCC ATCCTTGTCA ATCAAGGCGT TGGTCGCTTC CGGATTGTTT ACATAACCGG
  541 ACATAATCAT AGGTCCTCTG ACACATAATT CGCCTCTCTG ATTAACGCCC AGCGTTTTCC
  601 CGGTATCCAG ATCCACAACC TTCGCTTCAA AAAATGGAAC AACTTTACCG ACCGCGCCCG
  661 GTTTATCATC CCCCTCGGGT GTAATCAGAA TAGCTGATGT AGTCTCAGTG AGCCCATATC
  721 CTTGTCGTAT CCCTGGAAGA TGGAAGCGTT TTGCAACCGC TTCCCCGACT TCTTTCGAAA
  781 GAGGTGCGCC CCCAGAAGCA ATTTCGTGTA AATTAGATAA ATCGTATTTG TCAATCAGAG
  841 TGCTTTTGGC GAAGAATGAA AATAGGGTTG GTACTAGCAA CGCACTTTGA ATTTTGTAAT
  901 CCTGAAAGGG ATCGTAAAAA CAGCTCTTCT TCAAATCTAT ACATTAAGAC GACTCGAAAT
  961 CCACATATCA AATATCCGAG TGTAGTAAAC ATTCCCAAAA CCCGTGATGG AATGGAACAA
 1021 CACTTAAAAT CGCAGTATCC CGGGAATGAT TTGATTGCCA AAAAATAGGA TCTCTGGGCA
 1081 TGCGAGATCT GGACGGCAGC AGTCTATGCC GACGCCACAC CCTGTAGTTA CCTCAGTAGA
1141 AACTTCCAGA GGAGGGAATG A
//
Primer: Luc-M_R RDB14402_A6Kmc.seq
>06776_14402_A9FiF_Luc-M_R_A05_02_ABI24.ab1
    1 CATCCGGACT TCTTTCGAAG AGGTGCGCCC CCAGAAGCAA TTTCGTGTAA ATTAGATAAA
   61 TCGTATTTGT CAATCAGAGT GCTTTTGGCG AAGAATGAAA ATAGGGTTGG TACTAGCAAC
  121 GCACTTTGAA TTTTGTAATC CTGAAGGGAT CGTAAAAACA GCTCTTCTTC AAATCTATAC
  181 ATTAAGACGA CTCGAAATCC ACATATCAAA TATCCGAGTG TAGTAAACAT TCCAAAACCG
  241 TGATGGAATG GAACAACACT TAAAATCGCA GTATCCGGAA TGATTTGATT GCCAAAAATA
  301 GGATCTCTGG CATGCGAGAA TCTGACGCAG GCAGTTCTAT GCGGAAGGGC CACACCCTTA
  361 GGTAACCCAG TAGATCCAGA GGAATTCATT ATCAGTGCAA TTGTTTTGTC ACGATCAAAG
  421 GACTCTGGTA CAAAATCGTA TTCATTAAAA CCGGGAGGTA GATGAGATGT GACGAACGTG
  481 TACATCGACT GAAATCCCTG GTAATCCGTT TTAGAATCCA TGATAATAAT TTTCTGGATT
  541 ATTGGTAATT TTTTTTGCAC GTTCAAAATT TTTTGCAACC CCTTTTTGGA AACAAACACT
  601 ACGGTAGGCT GCGAAATGTT CATACTGTTG AGCAATTCAC GTTCATTATA AATGTCGTTC
  661 GCGGGCGCAA CTGCAACTCC GATAAATAAC GCGCCCAACA CCGGCATAAA GAATTGAAGA
  721 GAGTTTTCAC TGCATACGAC GATTCTGTGA TTTGTATTCA GCCCATATCG TTTCATAGCT
  781 TCTGCCAACC GAACGGACAT TTCGAAGTAT TCCGCGTACG TGATGTTCAC CTCGATATGT
  841 GCATCTGTAA AAGCAATTGT TCCAGGAACC AGGGCGTATC TCTTCATAGC CTTATGCAGT
  901 TGCTCTCCAG CGGTTCCATC CTCTAGAGGG ATAGAATGGC GCCCGGGCCT TTCTTTATGT
  961 TTTTGGGCGT CTTCCATTTT ACCAACAGTA CCGGAATGCC AAGCTTCCCT CGAGGGATTG
 1021 CAGTAAACAC AGGTTGTGTC AAAAGCAAGT GTAAGCAGAG CTGAGGTGAT CTGCCTTTTT
 1081 ATGCCCAGAT GCATTACCAG TGGAATCTAC CTATTTTTCC CCAACGGGGG TGCCCCCCAT
//
Primer: SV40polyA RDB14402_A6Kmd.seq
>06776_14402_A9FiF_SV40polyA_R2_B05_05_ABI24.ab1
    1 CTTAATAGGC CTGCAATAAC AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT
   61 TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC
  121 TGATTATGAT CTCTAGTCAA GGCACTATAC ATCAAATATT CCTTATTAAC CCCTTTACAA
  181 ATTAAAAAGC TAAAGGTACA CAATTTTTGA GCATAGTTAT TAATAGCAGA CACTCTATGC
  241 CTGTGTGGAG TAAGAAAAAA CAGTATGTTA TGATTATAAC TGTTATGCCT ACTTATAAAG
  301 GTTACAGAAT ATTTTTCCAT AATTTTCTTG TATAGCAGTG CAGCTTTTTC CTTTGTGGTG
  361 TAAATAGCAA AGCAAGCAAG AGTTCTATTA CTAAACACAG CATGACTCAA AAAACTTAGC
  421 AATTCTGAAG GAAAGTCCTT GGGGTCTTCT ACCTTTCTCT TCTTTTTTGG AGGAGTAGAA
  481 TGTTGAGAGT CAGCAGTAGC CTCATCATCA CTAGATGGCA TTTCTTCTGA GCAAAACAGG
  541 TTTTCCTCAT TAAAGGCATT CCACCACTGC TCCCATTCAT CAGTTCCATA GGTTGGAATC
  601 TAAAATACAC AAACAATTAG AATCAGTAGT TTAACACATT ATACACTTAA AAATTTTATA
  661 TTTACCTTAG AGCTTTAAAT CTCTGTAGGT AGTTTGTCCA ATTATGTCAC ACCACAGAAG
  721 TAAGGTTCCT TCACAAAGAT CCTCTAGAGT ATTACAATAG CTAAGAATTT CGTCATCGCT
  781 GAATACAGTT ACATTTTACA ATTTGGACTT TCCGCCCTTC TTGGCCTTTA TGAGGATCTC
  841 TCTGATTTTT CTTGCGTCGA GTTTTCCGGT AAGACCTTTC GGTACTTCGT TCCACAAACA
  901 CAACTCCTCC GCGCAACTTT TTTCGCGGTT GTTACTTGAC TGGCGACGTA ATCCACGATC
  961 TCTTTTTCCG TCATCGTCTT TCCGTGCTCC AAAACAACAA CGGCGGCGGG AAGTTCACCG
 1021 GGCGTCATCG TCGGGAAGAC CTGGCACGCC CCGCGTCGAA GATGTGGGTG TGGTTACAAA
 1081 TATCGGATTT CCAAATTCAG CGGGGCCAAC TTGATATCTT TTGGTAATTA AATTAAAAGG
1141 AACCTTTCA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kurooka, H., Roles of the ankyrin repeats and C-terminal region of the mouse notch1 intracellular region. Nucleic Acids Res., 6(23): 5448-5455 (1998). PMID 9826771.
original Minoguchi, S., RBP-L, a transcription factor related to RBP-Jkappa. Mol. Cell. Biol., 17, 2679-2687 (1997). PMID 9111338.
reference Itatani, Y., Characterization of Aes nuclear foci in colorectal cancer cells. J. Biochem. 159 (1): 133-140 (2016). PMID 26229111.
reference Shimizu, K., Integrity of intracellular domain of Notch ligand is indispensable for cleavage required for release of the Notch2 intracellular domain. EMBO J. 21 (3): 294-302 (2002). PMID 11823422.
reference Oswald, F., p300 acts as a transcriptional coactivator for mammalian Notch-1. Mol. Cell. Biol. 21 (22): 7761-7774 (2001). PMID 11604511.
user report Kawamoto, A., Ubiquitin D is up-regulated by synergy of Notch signalling and TNF-alpha in the inflamed intestinal epithelia of IBD patients. J Crohns Colitis. 2018 Nov 3. doi: 10.1093/ecco-jcc/jjy180. [Epub ahead of print] PMID 30395194.
user report Yoneyama, T., Notch Inhibitors from Calotropis gigantea That Induce Neuronal Differentiation of Neural Stem Cells. J. Nat. Prod. 80 (9): 2453-2461 (2017). PMID 28817274.
user report Sugiyama, M., Paired related homeobox 1 is associated with the invasive properties of glioblastoma cells. Oncol. Rep. 33 (3): 1123-1130 (2015). PMID 25522823.
user report Matsuda, M., Synthetic lateral inhibition governs cell-type bifurcation with robust ratios. Nat. Commun. 6: 6195 (2015). PMID 25652697.
user report O'Brien, L.L., Wt1a, Foxc1a, and the Notch mediator Rbpj physically interact and regulate the formation of podocytes in zebrafish. Dev. Biol., 358 (2): 318-330 (2011). PMID 21871448.

2019.06.24

GNP_filter3_RDBDEP_html_190430.pl