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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGa981-6 (#RDB06776)

Reporter construct containing the hexamerized 50 bp EBNA2 response element of the TP-1 promoter. TP1-luciferase reporter.

Alternative name TP1-luc(981-6) , #9507
Clone info. Reporter construct containing the hexamerized 50 bp EBNA2 response element of the TP-1 promoter.
Comment The response element was inserted in front of the mnimal beta-globin promoter of pGa50-7. Please refer Fig.1 of Oswald, F., Mol. Cell. Biol. 21 (22): 7761-7774 (2001).
Vector backbone pGa50-7 (Plasmid)
Selectable markers Amp^r
Growth conditions 37C, LB+Amp
Gene/insert name Artifical EBNA2 response element of the TP-1 synthesized
Depositor|Developer Honjo, Tasuku |
 
Remarks, protocol and/or map (pdf) RDB06776.pdf

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Nucleic Acids Res., 26, 5448-5455 (1998).). The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.
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手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Nucleic Acids Res., 26, 5448-5455 (1998).)。 公表を前提とした学術研究に限る。営利機関の利用者は、事前に京都大学・「医学領域」産学連携推進機構・知財室 (contracts@kumbl.med.kyoto-u.ac.jp) に連絡し、承諾を得ること。理研BRCは、本件リソースのリソース名、提供先、利用目的、件数を京都大学・本庶佑教授に報告する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06776 pGa981-6 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGa981-6 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06776).

Reference section:

Kurooka, H., Kuroda, K., Honjo, T., Roles of the ankyrin repeats and C-terminal region of the mouse notch1 intracellular region. Nucleic Acids Res., 6(23): 5448-5455 (1998). PMID 9826771. [link to RRC of NBRP]
Minoguchi, S., Taniguchi, Y., Kato, H., Okazaki, T., Strobl, L.J., Zimber-Strobl, U., Bornkamm, G.W., Honjo, T., RBP-L, a transcription factor related to RBP-Jkappa. Mol. Cell. Biol., 17, 2679-2687 (1997). PMID 9111338. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19971_B3Ecp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: Amp_R (Pr0008)
Region: 6xERE-TP1,mini beta-globin pro
Sequence file: RDB19971_B3Eca.seq check
>06776_19971_B3Ec_2_Amp_R_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNTGN NNCNNTNATN NGGGTTATTG TCTCATGAGC GGNTACATAT
   61 TTGAATGTAT TTNNNNNANT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
  121 CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA
  181 CGAGGCCTAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC
  241 GCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC
  301 TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG GTAACGCCAG
  361 GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGC CAAGCTTGCA TGCCTGCAGG
  421 TCGACTCTAG AGGATCCCGA CTCATGGGAA AATGGGCGGA AGGGCACCGT GGGAAAATAG
  481 TAGATCCCGA CTCGTGGGAA AATGGGCGGA AGGGCACCGT GGGAAAATAG TAGATCCCGA
  541 CTCGTGGGAA AATGGGCGGA AGGGCACCGT GGGAAAATAG TAGATCCCGA CTCATGGGAA
  601 AATGGGCGGA AGGGCACCGT GGGAAAATAG TAGATCCCGA CTCGTGGGAA AATGGGCGGA
  661 AGGGCACCGT GGGAAAATAG TAGATCCCGA CTCGTGGGAA AATGGGCGGA AGGGCACCGT
  721 GGGAAAATAG TAGATCCACT GTATGCATCT TGGGCATAAA AGGCAGATCA CTTCAGCTTC
  781 TGCTTACACT TGCTTTTGAC ACAACTGTGT TTACTTGCAA TCCNNCNANG GAGCTTGGNN
  841 TTCCNGTACT GNTNGGTAAA ATGGAAGANG CCNAAAACAT NNNGAAAGGC CCGGCGNCNN
  901 TTCTANNCNT NTANAGGANG GNNCCGCNGG ANANNNANNT GCNTNNNNTA TNNNNNNNNT
961 ACNNNNNNNN TNNNNNNNNN N
//
Primer: pMAMneo-Reverse (Pr0088)
Region: luc 3'
Sequence file: RDB19971_B3Ecb.seq check
>06776_19971_B3Ec_2_pMAMneo-Reverse_E01_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNCNCN NNNGTNNGGN TCCTTCNCNA AGNNCCTCTA GAGTATTACA
   61 ATAGCTAAGA NNTTCGTCAT CGCTGAATAC AGTTACATTT TACAATTTGG ACTTTCCGCC
  121 CTTCTTGGCC TTTATGAGGA TCTCTCTGAT TTTTCTTGCG TCGAGTTTTC CGGTAAGACC
  181 TTTCGGTACT TCGTCCACAA ACACAACTCC TCCGCGCAAC TTTTTCGCGG TTGTTACTTG
  241 ACTGGCGACG TAATCCACGA TCTCTTTTTC CGTCATCGTC TTTCCGTGCT CCAAAACAAC
  301 AACGGCGGCG GGAAGTTCAC CGGCGTCATC GTCGGGAAGA CCTGCCACGC CCGCGTCGAA
  361 GATGTTGGGG TGTTGTAACA ATATCGATTC CAATTCAGCG GGGGCCACCT GATATCCTTT
  421 GTATTTAATT AAAGACTTCA AGCGGTCAAC TATGAAGAAG TGTTCGTCTT CGTCCCAGTA
  481 AGCTATGTCT CCAGAATGTA GCCATCCATC CTTGTCAATC AAGGCGTTGG TCGCTTCCGG
  541 ATTGTTTACA TAACCGGACA TAATCATAGG TCCTCTGACA CATAATTCGC CTCTCTGATT
  601 AACGCCCAGC GTTTTCCCGG TATCCAGATC CACAACCTTC GCTTCAAAAA ATGGAACAAC
  661 TTTACCGACC GCGCCCGGTT TATCATCCCC CTCGGGTGTA ATCAGAATAG CTGATGTAGT
  721 CTCAGTGAGC CCATATCCTT GTCGTATCCC TGGAAGANGG AAGCGTTTTG CAACCGCTTC
  781 CNCGACTTCT TTCGAAAGAN GTGCGNNNNA GAAGCAATTT CGTGTAAATT AGATAAATCG
  841 TATTTGTCAA TCAGAGTGCT TTTGGCGAAN AATGAAAATA GGGTNGGNNC TAGCNACNNA
  901 CTTTNNNTTT NGTNNNNNNG NNNNNNTCGN NNAANNNGNT CNTNNNNNNA NNNNNNNNNN
//
Primer: Luc_inner_R2 (Pr1286)
Region: luc 5'
Sequence file: RDB19971_B3Ecc.seq check
>06776_19971_B3Ec_2_Luc_inner_R2_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNGNNG CANGCAGTTC TATGCGGAAG GGCCNCACCC
   61 TTAGGTAACC CAGTNNNTCC ANNGGAATTC ATTATCAGTG CAATTGTTTT GTCACGATCA
  121 AAGGACTCTG GTACAAAATC GTATTCATTA AAACCGGGAG GTAGATGAGA TGTGACGAAC
  181 GTGTACATCG ACTGAAATCC CTGGTAATCC GTTTTAGAAT CCATGATAAT AATTTTCTGG
  241 ATTATTGGTA ATTTTTTTTG CACGTTCAAA ATTTTTTGCA ACCCCTTTTT GGAAACAAAC
  301 ACTACGGTAG GCTGCGAAAT GTTCATACTG TTGAGCAATT CACGTTCATT ATAAATGTCG
  361 TTCGCGGGCG CAACTGCAAC TCCGATAAAT AACGCGCCCA ACACCGGCAT AAAGAATTGA
  421 AGAGAGTTTT CACTGCATAC GACGATTCTG TGATTTGTAT TCAGCCCATA TCGTTTCATA
  481 GCTTCTGCCA ACCGAACGGA CATTTCGAAG TATTCCGCGT ACGTGATGTT CACCTCGATA
  541 TGTGCATCTG TAAAAGCAAT TGTTCCAGGA ACCAGGGCGT ATCTCTTCAT AGCCTTATGC
  601 AGTTGCTCTC CAGCGGTTCC ATCCTCTAGA GGATAGAATG GCGCCGGGCC TTTCTTTATG
  661 TTTTTGGCGT CTTCCATTTT ACCAACAGTA CCGGAATGCC AAGCTCCCTC GAGGGANTGC
  721 AAGTAAACAC AGTTGTGTCA AAAGCAAGTG TAAGCAGAAG CTGANGTGAT CTGCCTTTTA
  781 TGCCCAAGAN GCATACAGTG GNTCTACTAT TTTCCNACGG TGCCCTTCCG CCNATTTTCC
  841 CACNAGTCGG GNNCTACTAT TTTCCCACNN NGCCCNTNNC GCCCATTTTC CCACGANNCN
  901 GGGATCNACN NNTTTCCNNN NGNNCCNTNN CGNCNNTTTT NCCCNNGANT CGGNNNNNAN
961 NNNTTTNNCN NNNGNNNNCN NNNNCNNNNC NNNTTTNNNN NNNNNN
//
Primer: Luc-M_R (Pr0632)
Region: luc mid
Sequence file: RDB19971_B3Ecd.seq check
>06776_19971_B3Ec_2_Luc-M_R_D01_10_ABI24.ab1
    1 NNNNNNNNNN NNNNTNNNNN NNGNGCGCCC CCNGAAGCAA TTTCGTGTAA ATTAGATAAA
   61 TCGTATTTGN CAATCAGAGT GCTTTTGGCG AAGAATGAAA ATAGGGTTGG TACTAGCAAC
  121 GCACTTTGAA TTTTGTAATC CTGAAGGGAT CGTAAAAACA GCTCTTCTTC AAATCTATAC
  181 ATTAAGACGA CTCGAAATCC ACATATCAAA TATCCGAGTG TAGTAAACAT TCCAAAACCG
  241 TGATGGAATG GAACAACACT TAAAATCGCA GTATCCGGAA TGATTTGATT GCCAAAAATA
  301 GGATCTCTGG CATGCGAGAA TCTGACGCAG GCAGTTCTAT GCGGAAGGGC CACACCCTTA
  361 GGTAACCCAG TAGATCCAGA GGAATTCATT ATCAGTGCAA TTGTTTTGTC ACGATCAAAG
  421 GACTCTGGTA CAAAATCGTA TTCATTAAAA CCGGGAGGTA GATGAGATGT GACGAACGTG
  481 TACATCGACT GAAATCCCTG GTAATCCGTT TTAGAATCCA TGATAATAAT TTTCTGGATT
  541 ATTGGTAATT TTTTTTGCAC GTTCAAAATT TTTTGCAACC CCTTTTTGGA AACAAACACT
  601 ACGGTAGGCT GCGAAATGTT CATACTGTTG AGCAATTCAC GTTCATTATA AATGTCGTTC
  661 GCGGGCGCAA CTGCAACTCC GATAAATAAC GCGCCCAACA CCGGCATAAA GAANTGAAGA
  721 GAGTTTTCAC TGCATACGAC GATTCTGTGA TTTGTATTCA GCCCATATCG TTTCATANCT
  781 TCTGNCAACC GAACGGANNT TTCGAANTAN TCNNCGNACG TGANGNTCNN CTCGANNTGN
  841 GCATNNGTAA AANCANNNGT TCCNNNNANN NANGGNGNAT CNNNNCNTAN NNTTNNGCAN
901 NNGNNNTNNN NNNNNTNNNN NNNNNN
//
Primer: Luc inner_F1 (Pr0617)
Region: luc mid
Sequence file: RDB19971_B3Ece.seq check
>06776_19971_B3Ec_2_Luc inner_F1_E01_05_ABI08.ab1
    1 NNNNNNNNNN NNNTTTGNNN CNGNNTCCTT TGNNCGTGAN NAAACAATTG CACTGATAAT
   61 GAATTCCTCT GGNNCNACTG GGTTACCTAA GGGTGTGGCC CTTCCGCATA GAACTGCCTG
  121 CGTCAGATTC TCGCATGCCA GAGATCCTAT TTTTGGCAAT CAAATCATTC CGGATACTGC
  181 GATTTTAAGT GTTGTTCCAT TCCATCACGG TTTTGGAATG TTTACTACAC TCGGATATTT
  241 GATATGTGGA TTTCGAGTCG TCTTAATGTA TAGATTTGAA GAAGAGCTGT TTTTACGATC
  301 CCTTCAGGAT TACAAAATTC AAAGTGCGTT GCTAGTACCA ACCCTATTTT CATTCTTCGC
  361 CAAAAGCACT CTGATTGACA AATACGATTT ATCTAATTTA CACGAAATTG CTTCTGGGGG
  421 CGCACCTCTT TCGAAAGAAG TCGGGGAAGC GGTTGCAAAA CGCTTCCATC TTCCAGGGAT
  481 ACGACAAGGA TATGGGCTCA CTGAGACTAC ATCAGCTATT CTGATTACAC CCGAGGGGGA
  541 TGATAAACCG GGCGCGGTCG GTAAAGTTGT TCCATTTTTT GAAGCGAAGG TTGTGGATCT
  601 GGATACCGGG AAAACGCTGG GCGTTAATCA GAGAGGCGAA TTATGTGTCA GAGGACCTAT
  661 GATTATGTCC GGTTATGTAA ACAATCCGGA AGCGACCAAC GCCTTGATTG ACAAGGNTGG
  721 ATGGCTACAT TCTGGAGACA TAGCTTACTG NGACGAAGAC GAACACTTCT TCATAGTTGA
  781 CCGCTTGAAG TCTTTANTTA AATACNAAGG ANATCNNGNN NCCCCCGCTG ANNTGGAATC
  841 NATATTGTTA CNACANCCCC NACNTCNTCN ANGCGGGCGN GGCNNNTCNN NCCGANNANG
901 ANGCNNGNNG AACTNNCNGN CNNNNNNNNN N
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kurooka, H., Roles of the ankyrin repeats and C-terminal region of the mouse notch1 intracellular region. Nucleic Acids Res., 6(23): 5448-5455 (1998). PMID 9826771. [link to RRC of NBRP]
original Minoguchi, S., RBP-L, a transcription factor related to RBP-Jkappa. Mol. Cell. Biol., 17, 2679-2687 (1997). PMID 9111338. [link to RRC of NBRP]
reference Itatani, Y., Characterization of Aes nuclear foci in colorectal cancer cells. J. Biochem. 159 (1): 133-140 (2016). PMID 26229111.
reference Shimizu, K., Integrity of intracellular domain of Notch ligand is indispensable for cleavage required for release of the Notch2 intracellular domain. EMBO J. 21 (3): 294-302 (2002). PMID 11823422.
reference Oswald, F., p300 acts as a transcriptional coactivator for mammalian Notch-1. Mol. Cell. Biol. 21 (22): 7761-7774 (2001). PMID 11604511.
user_report Carter, A.A., Structural features of the Notch ankyrin domain-Deltex WWE2 domain heterodimer determined by NMR spectroscopy and functional implications. Structure 31 (5): 584-594.e5 (2023). PMID 36977409. [link to RRC of NBRP]
user_report Kawamoto, A., Ubiquitin D is up-regulated by synergy of Notch signalling and TNF-alpha in the inflamed intestinal epithelia of IBD patients. J. Crohns Colitis 13 (4): 495-509 (2019). PMID 30395194. [link to RRC of NBRP]
user_report Yoneyama, T., Notch Inhibitors from Calotropis gigantea That Induce Neuronal Differentiation of Neural Stem Cells. J. Nat. Prod. 80 (9): 2453-2461 (2017). PMID 28817274. [link to RRC of NBRP]
user_report Sugiyama, M., Paired related homeobox 1 is associated with the invasive properties of glioblastoma cells. Oncol. Rep. 33 (3): 1123-1130 (2015). PMID 25522823. [link to RRC of NBRP]
user_report Matsuda, M., Synthetic lateral inhibition governs cell-type bifurcation with robust ratios. Nat. Commun. 6: 6195 (2015). PMID 25652697. [link to RRC of NBRP]
user_report O'Brien, L.L., Wt1a, Foxc1a, and the Notch mediator Rbpj physically interact and regulate the formation of podocytes in zebrafish. Dev. Biol., 358 (2): 318-330 (2011). PMID 21871448. [link to RRC of NBRP]