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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGa981-6

Reporter construct containing the hexamerized 50 bp EBNA2 response element of the TP-1 promoter. TP1-luciferase reporter.

Catalog number RDB06776
Resource name pGa981-6
Alternative name TP1-luc(981-6) , #9507
Clone info. Reporter construct containing the hexamerized 50 bp EBNA2 response element of the TP-1 promoter.
Comment The response element was inserted in front of the mnimal beta-globin promoter of pGa50-7. Please refer Fig.1 of Oswald, F., Mol. Cell. Biol. 21 (22): 7761-7774 (2001).
Vector backbone pGa50-7 (Plasmid)
Selectable markers Amp^r
Gene/insert name Artifical EBNA2 response element of the TP-1 synthesized
Depositor|Developer Honjo, Tasuku |
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External Database
Artifical EBNA2 response element of the TP-1

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Nucleic Acids Res., 26, 5448-5455 (1998).). The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Nucleic Acids Res., 26, 5448-5455 (1998).)。 公表を前提とした学術研究に限る。営利機関の利用者は、事前に京都大学・「医学領域」産学連携推進機構・知財室 (contracts@kumbl.med.kyoto-u.ac.jp) に連絡し、承諾を得ること。理研BRCは、本件リソースのリソース名、提供先、利用目的、件数を京都大学・本庶佑教授に報告する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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            RDB06776 pGa981-6 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Remarks, protocol and/or map (pdf) RDB06776.pdf

            Original reference

            original Kurooka, H., Roles of the ankyrin repeats and C-terminal region of the mouse notch1 intracellular region. Nucleic Acids Res., 6(23): 5448-5455 (1998). PMID 9826771.
            original Minoguchi, S., RBP-L, a transcription factor related to RBP-Jkappa. Mol. Cell. Biol., 17, 2679-2687 (1997). PMID 9111338.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Dnaconda's recommendation EXR0030j (Japanese text)
            Featured content Promoter Collection (Outline) (English text)
            Featured content Promoter Collection (Outline) (Japanese text)
            Featured content Notch signaling pathway (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14402_A6Kmp1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: Amp_R
            Sequence file: RDB14402_A6Kma.seq check
            >06776_14402_A6Km_Amp_R_A10_01_ABI24.ab1
                1 TTTCGTTTTA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
               61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
              121 CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG
              181 AGGCCTATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGC
              241 CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA
              301 TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG
              361 TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTGCCA AGCTTGCATG CCTGCAGGTC
              421 GACTCTAGAG GATCCCGACT CATGGGAAAA TGGGCGGAAG GGCACCGTGG GAAAATAGTA
              481 GATCCCGACT CGTGGGAAAA TGGGCGGAAG GGCACCGTGG GAAAATAGTA GATCCCGACT
              541 CGTGGGAAAA TGGGCGGAAG GGCACCGTGG GAAAATAGTA GATCCCGACT CATGGGAAAA
              601 TGGGCGGAAG GGCACCGTGG GAAAATAGTA GATCCCGACT CGTGGGAAAA TGGGCGGAAG
              661 GGCACCGTGG GAAAATAGTA GATCCCGACT CGTGGGAAAA TGGGCGGAAG GGCACCGTGG
              721 GAAAATAGTA GATCCACTGT ATGCATCTTG GGCATAAAAG GCAGATCACT TCAGCTTCTG
              781 CTTACACTTG CTTTTGACAC AACTGTGTTT ACTTGCAATC CCTCGAGGGA GCTTGGCATT
              841 CCGGTACTGT TGGTAAAATG GAAGACGCCA AAAACATAAA GAAAGGCCCG GCGCCATTCT
              901 ATCCTCTAGA GGATGGAACC GCTGGAGAGC AACTGCATAA GCTATGAAGA GATACGCCCC
              961 TGGTTCCTGG AACAATTGCT TTTACAGATG CACATATCGA GGGTGACATC ACGTACGCGG
             1021 ATACTTCGAA TGTCCGTTCA TGCAGAGCTA TGAAACGAAT ATGGGGCTGA TACAATCCAC
             1081 AGAATCGTCG TATGCCATGA ACTCTCTTCC ATTCCTTTAT TGCCGGTGTT TGGGGGGCGC
            1141 GGCG
            //
            Primer: SV40-3'UTR
            Sequence file: RDB14402_A6Kmb.seq check
            >06776_14402_A6Km_SV40-3'UTR_D03_12_ABI24.ab1
                1 TACAGTAGTA CGTTCCTTCA CAAAGATCCT CTAGAGTATT ACAATAGCTA AGAATTTCGT
               61 CATCGCTGAA TACAGTTACA TTTTACAATT TGGACTTTCC GCCCTTCTTG GCCTTTATGA
              121 GGATCTCTCT GATTTTTCTT GCGTCGAGTT TTCCGGTAAG ACCTTTCGGT ACTTCGTCCA
              181 CAAACACAAC TCCTCCGCGC AACTTTTTCG CGGTTGTTAC TTGACTGGCG ACGTAATCCA
              241 CGATCTCTTT TTCCGTCATC GTCTTTCCGT GCTCCAAAAC AACAACGGCG GCGGGAAGTT
              301 CACCGGCGTC ATCGTCGGGA AGACCTGCCA CGCCCGCGTC GAAGATGTTG GGGTGTTGTA
              361 ACAATATCGA TTCCAATTCA GCGGGGGCCA CCTGATATCC TTTGTATTTA ATTAAAGACT
              421 TCAAGCGGTC AACTATGAAG AAGTGTTCGT CTTCGTCCCA GTAAGCTATG TCTCCAGAAT
              481 GTAGCCATCC ATCCTTGTCA ATCAAGGCGT TGGTCGCTTC CGGATTGTTT ACATAACCGG
              541 ACATAATCAT AGGTCCTCTG ACACATAATT CGCCTCTCTG ATTAACGCCC AGCGTTTTCC
              601 CGGTATCCAG ATCCACAACC TTCGCTTCAA AAAATGGAAC AACTTTACCG ACCGCGCCCG
              661 GTTTATCATC CCCCTCGGGT GTAATCAGAA TAGCTGATGT AGTCTCAGTG AGCCCATATC
              721 CTTGTCGTAT CCCTGGAAGA TGGAAGCGTT TTGCAACCGC TTCCCCGACT TCTTTCGAAA
              781 GAGGTGCGCC CCCAGAAGCA ATTTCGTGTA AATTAGATAA ATCGTATTTG TCAATCAGAG
              841 TGCTTTTGGC GAAGAATGAA AATAGGGTTG GTACTAGCAA CGCACTTTGA ATTTTGTAAT
              901 CCTGAAAGGG ATCGTAAAAA CAGCTCTTCT TCAAATCTAT ACATTAAGAC GACTCGAAAT
              961 CCACATATCA AATATCCGAG TGTAGTAAAC ATTCCCAAAA CCCGTGATGG AATGGAACAA
             1021 CACTTAAAAT CGCAGTATCC CGGGAATGAT TTGATTGCCA AAAAATAGGA TCTCTGGGCA
             1081 TGCGAGATCT GGACGGCAGC AGTCTATGCC GACGCCACAC CCTGTAGTTA CCTCAGTAGA
            1141 AACTTCCAGA GGAGGGAATG A
            //
            Primer: Luc-M_R
            Sequence file: RDB14402_A6Kmc.seq check
            >06776_14402_A9FiF_Luc-M_R_A05_02_ABI24.ab1
                1 CATCCGGACT TCTTTCGAAG AGGTGCGCCC CCAGAAGCAA TTTCGTGTAA ATTAGATAAA
               61 TCGTATTTGT CAATCAGAGT GCTTTTGGCG AAGAATGAAA ATAGGGTTGG TACTAGCAAC
              121 GCACTTTGAA TTTTGTAATC CTGAAGGGAT CGTAAAAACA GCTCTTCTTC AAATCTATAC
              181 ATTAAGACGA CTCGAAATCC ACATATCAAA TATCCGAGTG TAGTAAACAT TCCAAAACCG
              241 TGATGGAATG GAACAACACT TAAAATCGCA GTATCCGGAA TGATTTGATT GCCAAAAATA
              301 GGATCTCTGG CATGCGAGAA TCTGACGCAG GCAGTTCTAT GCGGAAGGGC CACACCCTTA
              361 GGTAACCCAG TAGATCCAGA GGAATTCATT ATCAGTGCAA TTGTTTTGTC ACGATCAAAG
              421 GACTCTGGTA CAAAATCGTA TTCATTAAAA CCGGGAGGTA GATGAGATGT GACGAACGTG
              481 TACATCGACT GAAATCCCTG GTAATCCGTT TTAGAATCCA TGATAATAAT TTTCTGGATT
              541 ATTGGTAATT TTTTTTGCAC GTTCAAAATT TTTTGCAACC CCTTTTTGGA AACAAACACT
              601 ACGGTAGGCT GCGAAATGTT CATACTGTTG AGCAATTCAC GTTCATTATA AATGTCGTTC
              661 GCGGGCGCAA CTGCAACTCC GATAAATAAC GCGCCCAACA CCGGCATAAA GAATTGAAGA
              721 GAGTTTTCAC TGCATACGAC GATTCTGTGA TTTGTATTCA GCCCATATCG TTTCATAGCT
              781 TCTGCCAACC GAACGGACAT TTCGAAGTAT TCCGCGTACG TGATGTTCAC CTCGATATGT
              841 GCATCTGTAA AAGCAATTGT TCCAGGAACC AGGGCGTATC TCTTCATAGC CTTATGCAGT
              901 TGCTCTCCAG CGGTTCCATC CTCTAGAGGG ATAGAATGGC GCCCGGGCCT TTCTTTATGT
              961 TTTTGGGCGT CTTCCATTTT ACCAACAGTA CCGGAATGCC AAGCTTCCCT CGAGGGATTG
             1021 CAGTAAACAC AGGTTGTGTC AAAAGCAAGT GTAAGCAGAG CTGAGGTGAT CTGCCTTTTT
             1081 ATGCCCAGAT GCATTACCAG TGGAATCTAC CTATTTTTCC CCAACGGGGG TGCCCCCCAT
            //
            Primer: SV40polyA
            Sequence file: RDB14402_A6Kmd.seq check
            >06776_14402_A9FiF_SV40polyA_R2_B05_05_ABI24.ab1
                1 CTTAATAGGC CTGCAATAAC AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT
               61 TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC
              121 TGATTATGAT CTCTAGTCAA GGCACTATAC ATCAAATATT CCTTATTAAC CCCTTTACAA
              181 ATTAAAAAGC TAAAGGTACA CAATTTTTGA GCATAGTTAT TAATAGCAGA CACTCTATGC
              241 CTGTGTGGAG TAAGAAAAAA CAGTATGTTA TGATTATAAC TGTTATGCCT ACTTATAAAG
              301 GTTACAGAAT ATTTTTCCAT AATTTTCTTG TATAGCAGTG CAGCTTTTTC CTTTGTGGTG
              361 TAAATAGCAA AGCAAGCAAG AGTTCTATTA CTAAACACAG CATGACTCAA AAAACTTAGC
              421 AATTCTGAAG GAAAGTCCTT GGGGTCTTCT ACCTTTCTCT TCTTTTTTGG AGGAGTAGAA
              481 TGTTGAGAGT CAGCAGTAGC CTCATCATCA CTAGATGGCA TTTCTTCTGA GCAAAACAGG
              541 TTTTCCTCAT TAAAGGCATT CCACCACTGC TCCCATTCAT CAGTTCCATA GGTTGGAATC
              601 TAAAATACAC AAACAATTAG AATCAGTAGT TTAACACATT ATACACTTAA AAATTTTATA
              661 TTTACCTTAG AGCTTTAAAT CTCTGTAGGT AGTTTGTCCA ATTATGTCAC ACCACAGAAG
              721 TAAGGTTCCT TCACAAAGAT CCTCTAGAGT ATTACAATAG CTAAGAATTT CGTCATCGCT
              781 GAATACAGTT ACATTTTACA ATTTGGACTT TCCGCCCTTC TTGGCCTTTA TGAGGATCTC
              841 TCTGATTTTT CTTGCGTCGA GTTTTCCGGT AAGACCTTTC GGTACTTCGT TCCACAAACA
              901 CAACTCCTCC GCGCAACTTT TTTCGCGGTT GTTACTTGAC TGGCGACGTA ATCCACGATC
              961 TCTTTTTCCG TCATCGTCTT TCCGTGCTCC AAAACAACAA CGGCGGCGGG AAGTTCACCG
             1021 GGCGTCATCG TCGGGAAGAC CTGGCACGCC CCGCGTCGAA GATGTGGGTG TGGTTACAAA
             1081 TATCGGATTT CCAAATTCAG CGGGGCCAAC TTGATATCTT TTGGTAATTA AATTAAAAGG
            1141 AACCTTTCA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Kurooka, H., Roles of the ankyrin repeats and C-terminal region of the mouse notch1 intracellular region. Nucleic Acids Res., 6(23): 5448-5455 (1998). PMID 9826771.
            original Minoguchi, S., RBP-L, a transcription factor related to RBP-Jkappa. Mol. Cell. Biol., 17, 2679-2687 (1997). PMID 9111338.
            reference Itatani, Y., Characterization of Aes nuclear foci in colorectal cancer cells. J. Biochem. 159 (1): 133-140 (2016). PMID 26229111.
            reference Shimizu, K., Integrity of intracellular domain of Notch ligand is indispensable for cleavage required for release of the Notch2 intracellular domain. EMBO J. 21 (3): 294-302 (2002). PMID 11823422.
            reference Oswald, F., p300 acts as a transcriptional coactivator for mammalian Notch-1. Mol. Cell. Biol. 21 (22): 7761-7774 (2001). PMID 11604511.
            user_report Kawamoto, A., Ubiquitin D is up-regulated by synergy of Notch signalling and TNF-alpha in the inflamed intestinal epithelia of IBD patients. J. Crohns Colitis 13 (4): 495-509 (2019). PMID 30395194.
            user_report Yoneyama, T., Notch Inhibitors from Calotropis gigantea That Induce Neuronal Differentiation of Neural Stem Cells. J. Nat. Prod. 80 (9): 2453-2461 (2017). PMID 28817274.
            user_report Sugiyama, M., Paired related homeobox 1 is associated with the invasive properties of glioblastoma cells. Oncol. Rep. 33 (3): 1123-1130 (2015). PMID 25522823.
            user_report Matsuda, M., Synthetic lateral inhibition governs cell-type bifurcation with robust ratios. Nat. Commun. 6: 6195 (2015). PMID 25652697.
            user_report O'Brien, L.L., Wt1a, Foxc1a, and the Notch mediator Rbpj physically interact and regulate the formation of podocytes in zebrafish. Dev. Biol., 358 (2): 318-330 (2011). PMID 21871448.

            2022.05.18

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