Please review the QC test results indicated by icon below as well as clone information before placing your order.

pGa981-6

Reporter construct containing the hexamerized 50 bp EBNA2 response element of the TP-1 promoter. TP1-luciferase reporter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Nucleic Acids Res., 26, 5448-5455 (1998).). The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.

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Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Nucleic Acids Res., 26, 5448-5455 (1998).)。 公表を前提とした学術研究に限る。営利機関の利用者は、事前に京都大学・「医学領域」産学連携推進機構・知財室 (contracts@kumbl.med.kyoto-u.ac.jp) に連絡し、承諾を得ること。理研BRCは、本件リソースのリソース名、提供先、利用目的、件数を京都大学・本庶佑教授に報告する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19971_B3Ecp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Amp_R (Pr0008) Region: 6xERE-TP1,mini beta-globin pro Sequence file: RDB19971_B3Eca.seq
>06776_19971_B3Ec_2_Amp_R_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNTGN NNCNNTNATN NGGGTTATTG TCTCATGAGC GGNTACATAT    61 TTGAATGTAT TTNNNNNANT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC   121 CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA   181 CGAGGCCTAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC   241 GCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC   301 TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG GTAACGCCAG   361 GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGC CAAGCTTGCA TGCCTGCAGG   421 TCGACTCTAG AGGATCCCGA CTCATGGGAA AATGGGCGGA AGGGCACCGT GGGAAAATAG   481 TAGATCCCGA CTCGTGGGAA AATGGGCGGA AGGGCACCGT GGGAAAATAG TAGATCCCGA   541 CTCGTGGGAA AATGGGCGGA AGGGCACCGT GGGAAAATAG TAGATCCCGA CTCATGGGAA   601 AATGGGCGGA AGGGCACCGT GGGAAAATAG TAGATCCCGA CTCGTGGGAA AATGGGCGGA   661 AGGGCACCGT GGGAAAATAG TAGATCCCGA CTCGTGGGAA AATGGGCGGA AGGGCACCGT   721 GGGAAAATAG TAGATCCACT GTATGCATCT TGGGCATAAA AGGCAGATCA CTTCAGCTTC   781 TGCTTACACT TGCTTTTGAC ACAACTGTGT TTACTTGCAA TCCNNCNANG GAGCTTGGNN   841 TTCCNGTACT GNTNGGTAAA ATGGAAGANG CCNAAAACAT NNNGAAAGGC CCGGCGNCNN   901 TTCTANNCNT NTANAGGANG GNNCCGCNGG ANANNNANNT GCNTNNNNTA TNNNNNNNNT 961 ACNNNNNNNN TNNNNNNNNN N //
Primer: pMAMneo-Reverse (Pr0088) Region: luc 3' Sequence file: RDB19971_B3Ecb.seq
>06776_19971_B3Ec_2_pMAMneo-Reverse_E01_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNCNCN NNNGTNNGGN TCCTTCNCNA AGNNCCTCTA GAGTATTACA    61 ATAGCTAAGA NNTTCGTCAT CGCTGAATAC AGTTACATTT TACAATTTGG ACTTTCCGCC   121 CTTCTTGGCC TTTATGAGGA TCTCTCTGAT TTTTCTTGCG TCGAGTTTTC CGGTAAGACC   181 TTTCGGTACT TCGTCCACAA ACACAACTCC TCCGCGCAAC TTTTTCGCGG TTGTTACTTG   241 ACTGGCGACG TAATCCACGA TCTCTTTTTC CGTCATCGTC TTTCCGTGCT CCAAAACAAC   301 AACGGCGGCG GGAAGTTCAC CGGCGTCATC GTCGGGAAGA CCTGCCACGC CCGCGTCGAA   361 GATGTTGGGG TGTTGTAACA ATATCGATTC CAATTCAGCG GGGGCCACCT GATATCCTTT   421 GTATTTAATT AAAGACTTCA AGCGGTCAAC TATGAAGAAG TGTTCGTCTT CGTCCCAGTA   481 AGCTATGTCT CCAGAATGTA GCCATCCATC CTTGTCAATC AAGGCGTTGG TCGCTTCCGG   541 ATTGTTTACA TAACCGGACA TAATCATAGG TCCTCTGACA CATAATTCGC CTCTCTGATT   601 AACGCCCAGC GTTTTCCCGG TATCCAGATC CACAACCTTC GCTTCAAAAA ATGGAACAAC   661 TTTACCGACC GCGCCCGGTT TATCATCCCC CTCGGGTGTA ATCAGAATAG CTGATGTAGT   721 CTCAGTGAGC CCATATCCTT GTCGTATCCC TGGAAGANGG AAGCGTTTTG CAACCGCTTC   781 CNCGACTTCT TTCGAAAGAN GTGCGNNNNA GAAGCAATTT CGTGTAAATT AGATAAATCG   841 TATTTGTCAA TCAGAGTGCT TTTGGCGAAN AATGAAAATA GGGTNGGNNC TAGCNACNNA   901 CTTTNNNTTT NGTNNNNNNG NNNNNNTCGN NNAANNNGNT CNTNNNNNNA NNNNNNNNNN //
Primer: Luc_inner_R2 (Pr1286) Region: luc 5' Sequence file: RDB19971_B3Ecc.seq
>06776_19971_B3Ec_2_Luc_inner_R2_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNGNNG CANGCAGTTC TATGCGGAAG GGCCNCACCC    61 TTAGGTAACC CAGTNNNTCC ANNGGAATTC ATTATCAGTG CAATTGTTTT GTCACGATCA   121 AAGGACTCTG GTACAAAATC GTATTCATTA AAACCGGGAG GTAGATGAGA TGTGACGAAC   181 GTGTACATCG ACTGAAATCC CTGGTAATCC GTTTTAGAAT CCATGATAAT AATTTTCTGG   241 ATTATTGGTA ATTTTTTTTG CACGTTCAAA ATTTTTTGCA ACCCCTTTTT GGAAACAAAC   301 ACTACGGTAG GCTGCGAAAT GTTCATACTG TTGAGCAATT CACGTTCATT ATAAATGTCG   361 TTCGCGGGCG CAACTGCAAC TCCGATAAAT AACGCGCCCA ACACCGGCAT AAAGAATTGA   421 AGAGAGTTTT CACTGCATAC GACGATTCTG TGATTTGTAT TCAGCCCATA TCGTTTCATA   481 GCTTCTGCCA ACCGAACGGA CATTTCGAAG TATTCCGCGT ACGTGATGTT CACCTCGATA   541 TGTGCATCTG TAAAAGCAAT TGTTCCAGGA ACCAGGGCGT ATCTCTTCAT AGCCTTATGC   601 AGTTGCTCTC CAGCGGTTCC ATCCTCTAGA GGATAGAATG GCGCCGGGCC TTTCTTTATG   661 TTTTTGGCGT CTTCCATTTT ACCAACAGTA CCGGAATGCC AAGCTCCCTC GAGGGANTGC   721 AAGTAAACAC AGTTGTGTCA AAAGCAAGTG TAAGCAGAAG CTGANGTGAT CTGCCTTTTA   781 TGCCCAAGAN GCATACAGTG GNTCTACTAT TTTCCNACGG TGCCCTTCCG CCNATTTTCC   841 CACNAGTCGG GNNCTACTAT TTTCCCACNN NGCCCNTNNC GCCCATTTTC CCACGANNCN   901 GGGATCNACN NNTTTCCNNN NGNNCCNTNN CGNCNNTTTT NCCCNNGANT CGGNNNNNAN 961 NNNTTTNNCN NNNGNNNNCN NNNNCNNNNC NNNTTTNNNN NNNNNN //
Primer: Luc-M_R (Pr0632) Region: luc mid Sequence file: RDB19971_B3Ecd.seq
>06776_19971_B3Ec_2_Luc-M_R_D01_10_ABI24.ab1     1 NNNNNNNNNN NNNNTNNNNN NNGNGCGCCC CCNGAAGCAA TTTCGTGTAA ATTAGATAAA    61 TCGTATTTGN CAATCAGAGT GCTTTTGGCG AAGAATGAAA ATAGGGTTGG TACTAGCAAC   121 GCACTTTGAA TTTTGTAATC CTGAAGGGAT CGTAAAAACA GCTCTTCTTC AAATCTATAC   181 ATTAAGACGA CTCGAAATCC ACATATCAAA TATCCGAGTG TAGTAAACAT TCCAAAACCG   241 TGATGGAATG GAACAACACT TAAAATCGCA GTATCCGGAA TGATTTGATT GCCAAAAATA   301 GGATCTCTGG CATGCGAGAA TCTGACGCAG GCAGTTCTAT GCGGAAGGGC CACACCCTTA   361 GGTAACCCAG TAGATCCAGA GGAATTCATT ATCAGTGCAA TTGTTTTGTC ACGATCAAAG   421 GACTCTGGTA CAAAATCGTA TTCATTAAAA CCGGGAGGTA GATGAGATGT GACGAACGTG   481 TACATCGACT GAAATCCCTG GTAATCCGTT TTAGAATCCA TGATAATAAT TTTCTGGATT   541 ATTGGTAATT TTTTTTGCAC GTTCAAAATT TTTTGCAACC CCTTTTTGGA AACAAACACT   601 ACGGTAGGCT GCGAAATGTT CATACTGTTG AGCAATTCAC GTTCATTATA AATGTCGTTC   661 GCGGGCGCAA CTGCAACTCC GATAAATAAC GCGCCCAACA CCGGCATAAA GAANTGAAGA   721 GAGTTTTCAC TGCATACGAC GATTCTGTGA TTTGTATTCA GCCCATATCG TTTCATANCT   781 TCTGNCAACC GAACGGANNT TTCGAANTAN TCNNCGNACG TGANGNTCNN CTCGANNTGN   841 GCATNNGTAA AANCANNNGT TCCNNNNANN NANGGNGNAT CNNNNCNTAN NNTTNNGCAN 901 NNGNNNTNNN NNNNNTNNNN NNNNNN //
Primer: Luc inner_F1 (Pr0617) Region: luc mid Sequence file: RDB19971_B3Ece.seq
>06776_19971_B3Ec_2_Luc inner_F1_E01_05_ABI08.ab1     1 NNNNNNNNNN NNNTTTGNNN CNGNNTCCTT TGNNCGTGAN NAAACAATTG CACTGATAAT    61 GAATTCCTCT GGNNCNACTG GGTTACCTAA GGGTGTGGCC CTTCCGCATA GAACTGCCTG   121 CGTCAGATTC TCGCATGCCA GAGATCCTAT TTTTGGCAAT CAAATCATTC CGGATACTGC   181 GATTTTAAGT GTTGTTCCAT TCCATCACGG TTTTGGAATG TTTACTACAC TCGGATATTT   241 GATATGTGGA TTTCGAGTCG TCTTAATGTA TAGATTTGAA GAAGAGCTGT TTTTACGATC   301 CCTTCAGGAT TACAAAATTC AAAGTGCGTT GCTAGTACCA ACCCTATTTT CATTCTTCGC   361 CAAAAGCACT CTGATTGACA AATACGATTT ATCTAATTTA CACGAAATTG CTTCTGGGGG   421 CGCACCTCTT TCGAAAGAAG TCGGGGAAGC GGTTGCAAAA CGCTTCCATC TTCCAGGGAT   481 ACGACAAGGA TATGGGCTCA CTGAGACTAC ATCAGCTATT CTGATTACAC CCGAGGGGGA   541 TGATAAACCG GGCGCGGTCG GTAAAGTTGT TCCATTTTTT GAAGCGAAGG TTGTGGATCT   601 GGATACCGGG AAAACGCTGG GCGTTAATCA GAGAGGCGAA TTATGTGTCA GAGGACCTAT   661 GATTATGTCC GGTTATGTAA ACAATCCGGA AGCGACCAAC GCCTTGATTG ACAAGGNTGG   721 ATGGCTACAT TCTGGAGACA TAGCTTACTG NGACGAAGAC GAACACTTCT TCATAGTTGA   781 CCGCTTGAAG TCTTTANTTA AATACNAAGG ANATCNNGNN NCCCCCGCTG ANNTGGAATC   841 NATATTGTTA CNACANCCCC NACNTCNTCN ANGCGGGCGN GGCNNNTCNN NCCGANNANG 901 ANGCNNGNNG AACTNNCNGN CNNNNNNNNN N //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.29

GNP_filter3_RDBDEP_html_230512.pl