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HES-1-Luc-Reporter

Reporter construct of the mouse Hairy and enhancer of split 1.

Catalog number RDB06775
Resource name HES-1-Luc-Reporter
Clone info. Reporter construct of the mouse Hairy and enhancer of split 1. The -199 to +46 region was cloned, where +1 corresponds to +1 nt in Fig.1 on the reference (Takebayashi K., J. Biol. Chem. 269 (7): 5150-5156, 1994. PMID 7906273).
Vector backbone pGV-B (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Hairy and enhancer of split 1 genomic DNA
Depositor|Developer Honjo, Tasuku |
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External Database
Mouse Hairy and enhancer of split 1

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Development, 124, 4133-4141 (1997).). The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Development, 124, 4133-4141 (1997))。 公表を前提とした学術研究に限る。営利機関の利用者は、事前に京都大学・「医学領域」産学連携推進機構・知財室 (contracts@kumbl.med.kyoto-u.ac.jp) に連絡し、承諾を得ること。理研BRCは、本件リソースのリソース名、提供先、利用目的、件数を京都大学・本庶佑教授に報告する。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB06775 HES-1-Luc-Reporter DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Kato, H., Involvement of RBP-J in biological functions of mouse Notch1 and its derivatives. Development, 124 (20): 4133-4141 (1997). PMID 9374409.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Outline) (Japanese text)
          Featured content Notch signaling pathway (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17469_A9Gpp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: RV6R
          Region: insert full seq.
          Sequence file: RDB17469_A9Gpa.seq check
          >06775_17469_A9Gp_RV6R_D02_11_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNGN CGCCNGGCCT TTCTTTNTGT TTTTGGCGTC TTCCNTTTTA
             61 CCAACAGTAC CGGAATGCCA AGCTTACTTA GATCTCGACC AGACAGGGGA TTCCGCTGTT
            121 ATCAGCACCA GCTCCAGATC CTGTGTGATC CGCAGGCCCT GGCGGCCTCT ATATATATCT
            181 GGGACTGCAC GCGAACGGCT CGTGTGAAAC TTCCCAAACT TTCTTTCCCA CAGTAACTTT
            241 CAGCCAATGG GAGGAAGAGA CACGCGGCAC CGCTCGTGGA CTGCGCCCCC CCATTGGCCG
            301 CTAGGCACAA GGTCTGGCGG CCAATGGCGC GCGCCTGAGC CCGGGTTATG TTAGCTCAGT
            361 TACAGTACCA TAAGATACAT TGATGAGTTT GGACAAACCA CAACTAGAAT GCAGTGAAAA
            421 AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT TATAAGCTGC
            481 AATAAACAAG TTAACAACAA CAATTGCATT CATTTTATGT TTCAGGTTCA GGGGGAGGTG
            541 TGGGAGGTTT TTTAAAGCAA GTAAAACCTC CACGTACCTT AATATTACTT ACTTATCATG
            601 GTAGCTTGGG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC
            661 GCAGCCTGAA TGGCGAATGG CAAATTGTAA GCGTTAATAT TTTGTTAAAA TTCGCGTTAA
            721 ATTTTTGTTA AATCAGCTCA TTTTTTAACC AATAGGCCGA AATCGGCAAA ATCCCTTATA
            781 AATCAAAAGA ATAGACCGAG ATAGGGTTGA GTGTTGTTCC AGTTTGGAAC NAGANTCCAC
            841 TATTAAAGAA CGTGNNCTCC NACGNCNAAG GGCGAAAAAN CGNCTATCAN GGCGATGGNC
            901 CNCTACGNNA ACCATCACCN NNATCAAGTT TTTTNNGGGN CGAGGNGNCG NAAAGNACTN
          961 ANTCNGNANN NNNNANNGNN NCCNCCNGNT TTAGANCNNN NNNNNNN
          //
          Primer: pGL4pA_R
          Region: SV40p(A),SV40NLS
          Sequence file: RDB17469_A9Gpb.seq check
          >06775_17469_A9Gp_pGL4pA_R_E02_14_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NGNNNTGNNA AGNNACNTTG NTGAGTTTGG ACNAACCACA
             61 ACTAGAATGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT TGCTTTATTT
            121 GTAACCATTA TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT
            181 CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT
            241 ATGGCTGATT ATGATCTCTA GTCAAGGCAC TATACATCAA ATATTCCTTA TTAACCCCTT
            301 TACAAATTAA AAAGCTAAAG GTACACAATT TTTGAGCATA GTTATTAATA GCAGACACTC
            361 TATGCCTGTG TGGAGTAAGA AAAAACAGTA TGTTATGATT ATAACTGTTA TGCCTACTTA
            421 TAAAGGTTAC AGAATATTTT TCCATAATTT TCTTGTATAG CAGTGCAGCT TTTTCCTTTG
            481 TGGTGTAAAT AGCAAAGCAA GCAAGAGTTC TATTACTAAA CACAGCATGA CTCAAAAAAC
            541 TTAGCAATTC TGAAGGAAAG TCCTTGGGGT CTTCTACCTT TCTCTTCTTT TTTGGAGGAG
            601 TAGAATGTTG AGAGTCAGCA GTAGCCTCAT CATCACTAGA TGGCATTTCT TCTGAGCAAA
            661 ACAGGTTTTC CTCATTAAAG GCATTCCACC ACTGCTCCCA TTCATCAGTT CCATAGGTTG
            721 GAATCTAAAA TACACAAACA ATTAGAATCA GTAGTTTAAC ACATTATACA CTTAAAAATT
            781 TTATATTTAC CTTANAGCTT TAAATCTCTG TANGTAGTTT GTCCAATTAT GTCACACCAC
            841 AGAANTAANG TTCNTTCACA AAGNTCCGGC GAATTTCTGN CATTCATCCG CTTATTATCA
            901 CTTATTCAGG NGNANCAANN NGNNGTTNNA NNNNCACNNA NAACNGCNNT AAAAAAATTA
          961 NNNCCCNGNC NNNNNNNNCA TCGNNNNANN NNNNNNNN
          //
          Primer: SV40-3'UTR
          Region: Luciferase3'
          Sequence file: RDB17469_A9Gpc.seq check
          >06775_17469_A9Gp_SV40-3'UTR_A10_01_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNT NNNNNAGNNC CGGCNAANTT CTGCCNTTCN TCCGCTTATT
             61 ATCACTTATT CAGGCGTAGC AACCAGGCGT TTAAGGGCAC CAATAACTGC CTTAAAAAAA
            121 TTACGCCCCG CCCTGCCACT CATCGCAGTA TTACAATAGC TAAGAATTTC GTCATCGCTG
            181 AATACAGTTA CATTTTACAA TTTGGACTTT CCGCCCTTCT TGGCCTTTAT GAGGATCTCT
            241 CTGATTTTTC TTGCGTCGAG TTTTCCGGTA AGACCTTTCG GTACTTCGTC CACAAACACA
            301 ACTCCTCCGC GCAACTTTTT CGCGGTTGTT ACTTGACTGG CGACGTAATC CACGATCTCT
            361 TTTTCCGTCA TCGTCTTTCC GTGCTCCAAA ACAACAACGG CGGCGGGAAG TTCACCGGCG
            421 TCATCGTCGG GAAGACCTGC CACGCCCGCG TCGAAGATGT TGGGGTGTTG TAACAATATC
            481 GATTCCAATT CAGCGGGGGC CACCTGATAT CCTTTGTATT TAATTAAAGA CTTCAAGCGG
            541 TCAACTATGA AGAAGTGTTC GTCTTCGTCC CAGTAAGCTA TGTCTCCAGA ATGTAGCCAT
            601 CNATCCTTGT CAATCAAGGC GTTGGTCGCT TCCGGATTGT TTACATAACC GGACATAATC
            661 ATAGGTCCTC TGACACATAA TTCGCCTCTC TGATTAACGC CCAGCGTTTT CCCGGTATCC
            721 AGATCCACAA CCTTCGCTTC AAAAAATGGA ACAACTTTAC CGACCGCGCC CGGTTTATCA
            781 TCCCCCTCGG GTGTAATCAG AATAGCTGAT GTAGTCTCAG TGAGCNNATA TCCTTGTCGT
            841 ATCCCNGNNA GANGNAANCG TTTNGCAACC GCTTCCNNNA CTTCNTTCGA NNNNNNGNGN
            901 GCCCCNNNAA NCAATTNCNN GNNAATTAGA TAAATCNNAN TNNNNNANTC ANNNNNNNTT
          961 TNNNNNNNNN N
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kato, H., Involvement of RBP-J in biological functions of mouse Notch1 and its derivatives. Development, 124 (20): 4133-4141 (1997). PMID 9374409.
          user report Saito, Y., Casitas B-cell lymphoma mutation in childhood T-cell acute lymphoblastic leukemia. Leuk Res., 36 (8): 1009-1015 (2012). PMID 22591685.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl