Please review the QC test results indicated by icon below as well as clone information before placing your order.

HES-1-Luc-Reporter

Reporter construct of the mouse Hairy and enhancer of split 1.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Development, 124, 4133-4141 (1997).). The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.

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Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Development, 124, 4133-4141 (1997))。 公表を前提とした学術研究に限る。営利機関の利用者は、事前に京都大学・「医学領域」産学連携推進機構・知財室 (contracts@kumbl.med.kyoto-u.ac.jp) に連絡し、承諾を得ること。理研BRCは、本件リソースのリソース名、提供先、利用目的、件数を京都大学・本庶佑教授に報告する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17469_A9Gpp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: RV6R () Region: insert full seq. Sequence file: RDB17469_A9Gpa.seq
>06775_17469_A9Gp_RV6R_D02_11_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNGN CGCCNGGCCT TTCTTTNTGT TTTTGGCGTC TTCCNTTTTA    61 CCAACAGTAC CGGAATGCCA AGCTTACTTA GATCTCGACC AGACAGGGGA TTCCGCTGTT   121 ATCAGCACCA GCTCCAGATC CTGTGTGATC CGCAGGCCCT GGCGGCCTCT ATATATATCT   181 GGGACTGCAC GCGAACGGCT CGTGTGAAAC TTCCCAAACT TTCTTTCCCA CAGTAACTTT   241 CAGCCAATGG GAGGAAGAGA CACGCGGCAC CGCTCGTGGA CTGCGCCCCC CCATTGGCCG   301 CTAGGCACAA GGTCTGGCGG CCAATGGCGC GCGCCTGAGC CCGGGTTATG TTAGCTCAGT   361 TACAGTACCA TAAGATACAT TGATGAGTTT GGACAAACCA CAACTAGAAT GCAGTGAAAA   421 AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT TATAAGCTGC   481 AATAAACAAG TTAACAACAA CAATTGCATT CATTTTATGT TTCAGGTTCA GGGGGAGGTG   541 TGGGAGGTTT TTTAAAGCAA GTAAAACCTC CACGTACCTT AATATTACTT ACTTATCATG   601 GTAGCTTGGG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC   661 GCAGCCTGAA TGGCGAATGG CAAATTGTAA GCGTTAATAT TTTGTTAAAA TTCGCGTTAA   721 ATTTTTGTTA AATCAGCTCA TTTTTTAACC AATAGGCCGA AATCGGCAAA ATCCCTTATA   781 AATCAAAAGA ATAGACCGAG ATAGGGTTGA GTGTTGTTCC AGTTTGGAAC NAGANTCCAC   841 TATTAAAGAA CGTGNNCTCC NACGNCNAAG GGCGAAAAAN CGNCTATCAN GGCGATGGNC   901 CNCTACGNNA ACCATCACCN NNATCAAGTT TTTTNNGGGN CGAGGNGNCG NAAAGNACTN 961 ANTCNGNANN NNNNANNGNN NCCNCCNGNT TTAGANCNNN NNNNNNN //
Primer: pGL4pA_R () Region: SV40p(A),SV40NLS Sequence file: RDB17469_A9Gpb.seq
>06775_17469_A9Gp_pGL4pA_R_E02_14_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NGNNNTGNNA AGNNACNTTG NTGAGTTTGG ACNAACCACA    61 ACTAGAATGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT TGCTTTATTT   121 GTAACCATTA TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT   181 CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT   241 ATGGCTGATT ATGATCTCTA GTCAAGGCAC TATACATCAA ATATTCCTTA TTAACCCCTT   301 TACAAATTAA AAAGCTAAAG GTACACAATT TTTGAGCATA GTTATTAATA GCAGACACTC   361 TATGCCTGTG TGGAGTAAGA AAAAACAGTA TGTTATGATT ATAACTGTTA TGCCTACTTA   421 TAAAGGTTAC AGAATATTTT TCCATAATTT TCTTGTATAG CAGTGCAGCT TTTTCCTTTG   481 TGGTGTAAAT AGCAAAGCAA GCAAGAGTTC TATTACTAAA CACAGCATGA CTCAAAAAAC   541 TTAGCAATTC TGAAGGAAAG TCCTTGGGGT CTTCTACCTT TCTCTTCTTT TTTGGAGGAG   601 TAGAATGTTG AGAGTCAGCA GTAGCCTCAT CATCACTAGA TGGCATTTCT TCTGAGCAAA   661 ACAGGTTTTC CTCATTAAAG GCATTCCACC ACTGCTCCCA TTCATCAGTT CCATAGGTTG   721 GAATCTAAAA TACACAAACA ATTAGAATCA GTAGTTTAAC ACATTATACA CTTAAAAATT   781 TTATATTTAC CTTANAGCTT TAAATCTCTG TANGTAGTTT GTCCAATTAT GTCACACCAC   841 AGAANTAANG TTCNTTCACA AAGNTCCGGC GAATTTCTGN CATTCATCCG CTTATTATCA   901 CTTATTCAGG NGNANCAANN NGNNGTTNNA NNNNCACNNA NAACNGCNNT AAAAAAATTA 961 NNNCCCNGNC NNNNNNNNCA TCGNNNNANN NNNNNNNN //
Primer: SV40-3'UTR () Region: Luciferase3' Sequence file: RDB17469_A9Gpc.seq
>06775_17469_A9Gp_SV40-3'UTR_A10_01_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNT NNNNNAGNNC CGGCNAANTT CTGCCNTTCN TCCGCTTATT    61 ATCACTTATT CAGGCGTAGC AACCAGGCGT TTAAGGGCAC CAATAACTGC CTTAAAAAAA   121 TTACGCCCCG CCCTGCCACT CATCGCAGTA TTACAATAGC TAAGAATTTC GTCATCGCTG   181 AATACAGTTA CATTTTACAA TTTGGACTTT CCGCCCTTCT TGGCCTTTAT GAGGATCTCT   241 CTGATTTTTC TTGCGTCGAG TTTTCCGGTA AGACCTTTCG GTACTTCGTC CACAAACACA   301 ACTCCTCCGC GCAACTTTTT CGCGGTTGTT ACTTGACTGG CGACGTAATC CACGATCTCT   361 TTTTCCGTCA TCGTCTTTCC GTGCTCCAAA ACAACAACGG CGGCGGGAAG TTCACCGGCG   421 TCATCGTCGG GAAGACCTGC CACGCCCGCG TCGAAGATGT TGGGGTGTTG TAACAATATC   481 GATTCCAATT CAGCGGGGGC CACCTGATAT CCTTTGTATT TAATTAAAGA CTTCAAGCGG   541 TCAACTATGA AGAAGTGTTC GTCTTCGTCC CAGTAAGCTA TGTCTCCAGA ATGTAGCCAT   601 CNATCCTTGT CAATCAAGGC GTTGGTCGCT TCCGGATTGT TTACATAACC GGACATAATC   661 ATAGGTCCTC TGACACATAA TTCGCCTCTC TGATTAACGC CCAGCGTTTT CCCGGTATCC   721 AGATCCACAA CCTTCGCTTC AAAAAATGGA ACAACTTTAC CGACCGCGCC CGGTTTATCA   781 TCCCCCTCGG GTGTAATCAG AATAGCTGAT GTAGTCTCAG TGAGCNNATA TCCTTGTCGT   841 ATCCCNGNNA GANGNAANCG TTTNGCAACC GCTTCCNNNA CTTCNTTCGA NNNNNNGNGN   901 GCCCCNNNAA NCAATTNCNN GNNAATTAGA TAAATCNNAN TNNNNNANTC ANNNNNNNTT 961 TNNNNNNNNN N //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.02

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