Resource data sheet
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HES-1-Luc-Reporter

Reporter construct of the mouse Hairy and enhancer of split 1.

Catalog number RDB06775
Resource name HES-1-Luc-Reporter
Clone info. Reporter construct of the mouse Hairy and enhancer of split 1. The -190 to +60 of the promoter was inserted in the pGV-B vector.
Vector backbone pGV-B (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Hairy and enhancer of split 1 genomic DNA
Depositor Honjo, Tasuku |
 
Other clones in our bank mouse Hes1 (NCBI Gene 15205) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Development, 124, 4133-4141 (1997).). The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06775 HES-1-Luc-Reporter DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Kato, H., Involvement of RBP-J in biological functions of mouse Notch1 and its derivatives. Development, 124 (20): 4133-4141 (1997). PMID 9374409.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Promoter Collection (Outline) (Japanese text)
Featured content Notch signaling pathway (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB17469_A9Gpp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: RV6R | Sequence: RDB17469_A9Gpa.seq
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Region: SV40p(A),SV40NLS | Primer: pGL4pA_R | Sequence: RDB17469_A9Gpb.seq
>06775_17469_A9Gp_pGL4pA_R_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGNNNTGNNA AGNNACNTTG NTGAGTTTGG ACNAACCACA
   61 ACTAGAATGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT TGCTTTATTT
  121 GTAACCATTA TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT
  181 CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT
  241 ATGGCTGATT ATGATCTCTA GTCAAGGCAC TATACATCAA ATATTCCTTA TTAACCCCTT
  301 TACAAATTAA AAAGCTAAAG GTACACAATT TTTGAGCATA GTTATTAATA GCAGACACTC
  361 TATGCCTGTG TGGAGTAAGA AAAAACAGTA TGTTATGATT ATAACTGTTA TGCCTACTTA
  421 TAAAGGTTAC AGAATATTTT TCCATAATTT TCTTGTATAG CAGTGCAGCT TTTTCCTTTG
  481 TGGTGTAAAT AGCAAAGCAA GCAAGAGTTC TATTACTAAA CACAGCATGA CTCAAAAAAC
  541 TTAGCAATTC TGAAGGAAAG TCCTTGGGGT CTTCTACCTT TCTCTTCTTT TTTGGAGGAG
  601 TAGAATGTTG AGAGTCAGCA GTAGCCTCAT CATCACTAGA TGGCATTTCT TCTGAGCAAA
  661 ACAGGTTTTC CTCATTAAAG GCATTCCACC ACTGCTCCCA TTCATCAGTT CCATAGGTTG
  721 GAATCTAAAA TACACAAACA ATTAGAATCA GTAGTTTAAC ACATTATACA CTTAAAAATT
  781 TTATATTTAC CTTANAGCTT TAAATCTCTG TANGTAGTTT GTCCAATTAT GTCACACCAC
  841 AGAANTAANG TTCNTTCACA AAGNTCCGGC GAATTTCTGN CATTCATCCG CTTATTATCA
  901 CTTATTCAGG NGNANCAANN NGNNGTTNNA NNNNCACNNA NAACNGCNNT AAAAAAATTA
  961 NNNCCCNGNC NNNNNNNNCA TCGNNNNANN NNNNNNNNNN ANNNNNTNNN NNNNNNNNNN
 1021 NNNNNNNTNN CNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNN
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Region: Luciferase3' | Primer: SV40-3'UTR | Sequence: RDB17469_A9Gpc.seq
>06775_17469_A9Gp_SV40-3'UTR_A10_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNT NNNNNAGNNC CGGCNAANTT CTGCCNTTCN TCCGCTTATT
   61 ATCACTTATT CAGGCGTAGC AACCAGGCGT TTAAGGGCAC CAATAACTGC CTTAAAAAAA
  121 TTACGCCCCG CCCTGCCACT CATCGCAGTA TTACAATAGC TAAGAATTTC GTCATCGCTG
  181 AATACAGTTA CATTTTACAA TTTGGACTTT CCGCCCTTCT TGGCCTTTAT GAGGATCTCT
  241 CTGATTTTTC TTGCGTCGAG TTTTCCGGTA AGACCTTTCG GTACTTCGTC CACAAACACA
  301 ACTCCTCCGC GCAACTTTTT CGCGGTTGTT ACTTGACTGG CGACGTAATC CACGATCTCT
  361 TTTTCCGTCA TCGTCTTTCC GTGCTCCAAA ACAACAACGG CGGCGGGAAG TTCACCGGCG
  421 TCATCGTCGG GAAGACCTGC CACGCCCGCG TCGAAGATGT TGGGGTGTTG TAACAATATC
  481 GATTCCAATT CAGCGGGGGC CACCTGATAT CCTTTGTATT TAATTAAAGA CTTCAAGCGG
  541 TCAACTATGA AGAAGTGTTC GTCTTCGTCC CAGTAAGCTA TGTCTCCAGA ATGTAGCCAT
  601 CNATCCTTGT CAATCAAGGC GTTGGTCGCT TCCGGATTGT TTACATAACC GGACATAATC
  661 ATAGGTCCTC TGACACATAA TTCGCCTCTC TGATTAACGC CCAGCGTTTT CCCGGTATCC
  721 AGATCCACAA CCTTCGCTTC AAAAAATGGA ACAACTTTAC CGACCGCGCC CGGTTTATCA
  781 TCCCCCTCGG GTGTAATCAG AATAGCTGAT GTAGTCTCAG TGAGCNNATA TCCTTGTCGT
  841 ATCCCNGNNA GANGNAANCG TTTNGCAACC GCTTCCNNNA CTTCNTTCGA NNNNNNGNGN
  901 GCCCCNNNAA NCAATTNCNN GNNAATTAGA TAAATCNNAN TNNNNNANTC ANNNNNNNTT
  961 TNNNNNNNNN NNNNAAANNN GNNNNGNNNN NANNNNNNNN NNNNNNNNNN NNNNNNNNNN
 1021 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN N
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Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kato, H., Involvement of RBP-J in biological functions of mouse Notch1 and its derivatives. Development, 124 (20): 4133-4141 (1997). PMID 9374409.
user report Saito, Y., Casitas B-cell lymphoma mutation in childhood T-cell acute lymphoblastic leukemia. Leuk Res., 36 (8): 1009-1015 (2012). PMID 22591685.

2019.08.27

GNP_filter3_RDBDEP_html_190816.pl