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HES-1-Luc-Reporter (#RDB06775)

Reporter construct of the mouse Hairy and enhancer of split 1.

Clone info. Reporter construct of the mouse Hairy and enhancer of split 1. The -199 to +46 region was cloned, where +1 corresponds to +1 nt in Fig.1 on the reference (Takebayashi K., J. Biol. Chem. 269 (7): 5150-5156, 1994. PMID 7906273).
Vector backbone pGV-B (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Hairy and enhancer of split 1 genomic DNA
Depositor|Developer Honjo, Tasuku |
 

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Development, 124, 4133-4141 (1997).). The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Development, 124, 4133-4141 (1997))。 公表を前提とした学術研究に限る。営利機関の利用者は、事前に京都大学・「医学領域」産学連携推進機構・知財室 (contracts@kumbl.med.kyoto-u.ac.jp) に連絡し、承諾を得ること。理研BRCは、本件リソースのリソース名、提供先、利用目的、件数を京都大学・本庶佑教授に報告する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06775 HES-1-Luc-Reporter DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The HES-1-Luc-Reporter was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06775).

Reference section:

Kato, H., Taniguchi, Y., Kurooka, H., Minoguchi, S., Sakai, T., Nomura-Okazaki, S., Tamura, K., Honjo, T., Involvement of RBP-J in biological functions of mouse Notch1 and its derivatives. Development, 124 (20): 4133-4141 (1997). PMID 9374409. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17469_A9Gpp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: RV6R ()
Region: insert full seq.
Sequence file: RDB17469_A9Gpa.seq check
>06775_17469_A9Gp_RV6R_D02_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGN CGCCNGGCCT TTCTTTNTGT TTTTGGCGTC TTCCNTTTTA
   61 CCAACAGTAC CGGAATGCCA AGCTTACTTA GATCTCGACC AGACAGGGGA TTCCGCTGTT
  121 ATCAGCACCA GCTCCAGATC CTGTGTGATC CGCAGGCCCT GGCGGCCTCT ATATATATCT
  181 GGGACTGCAC GCGAACGGCT CGTGTGAAAC TTCCCAAACT TTCTTTCCCA CAGTAACTTT
  241 CAGCCAATGG GAGGAAGAGA CACGCGGCAC CGCTCGTGGA CTGCGCCCCC CCATTGGCCG
  301 CTAGGCACAA GGTCTGGCGG CCAATGGCGC GCGCCTGAGC CCGGGTTATG TTAGCTCAGT
  361 TACAGTACCA TAAGATACAT TGATGAGTTT GGACAAACCA CAACTAGAAT GCAGTGAAAA
  421 AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT TATAAGCTGC
  481 AATAAACAAG TTAACAACAA CAATTGCATT CATTTTATGT TTCAGGTTCA GGGGGAGGTG
  541 TGGGAGGTTT TTTAAAGCAA GTAAAACCTC CACGTACCTT AATATTACTT ACTTATCATG
  601 GTAGCTTGGG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC
  661 GCAGCCTGAA TGGCGAATGG CAAATTGTAA GCGTTAATAT TTTGTTAAAA TTCGCGTTAA
  721 ATTTTTGTTA AATCAGCTCA TTTTTTAACC AATAGGCCGA AATCGGCAAA ATCCCTTATA
  781 AATCAAAAGA ATAGACCGAG ATAGGGTTGA GTGTTGTTCC AGTTTGGAAC NAGANTCCAC
  841 TATTAAAGAA CGTGNNCTCC NACGNCNAAG GGCGAAAAAN CGNCTATCAN GGCGATGGNC
  901 CNCTACGNNA ACCATCACCN NNATCAAGTT TTTTNNGGGN CGAGGNGNCG NAAAGNACTN
961 ANTCNGNANN NNNNANNGNN NCCNCCNGNT TTAGANCNNN NNNNNNN
//
Primer: pGL4pA_R ()
Region: SV40p(A),SV40NLS
Sequence file: RDB17469_A9Gpb.seq check
>06775_17469_A9Gp_pGL4pA_R_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGNNNTGNNA AGNNACNTTG NTGAGTTTGG ACNAACCACA
   61 ACTAGAATGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT TGCTTTATTT
  121 GTAACCATTA TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT
  181 CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT
  241 ATGGCTGATT ATGATCTCTA GTCAAGGCAC TATACATCAA ATATTCCTTA TTAACCCCTT
  301 TACAAATTAA AAAGCTAAAG GTACACAATT TTTGAGCATA GTTATTAATA GCAGACACTC
  361 TATGCCTGTG TGGAGTAAGA AAAAACAGTA TGTTATGATT ATAACTGTTA TGCCTACTTA
  421 TAAAGGTTAC AGAATATTTT TCCATAATTT TCTTGTATAG CAGTGCAGCT TTTTCCTTTG
  481 TGGTGTAAAT AGCAAAGCAA GCAAGAGTTC TATTACTAAA CACAGCATGA CTCAAAAAAC
  541 TTAGCAATTC TGAAGGAAAG TCCTTGGGGT CTTCTACCTT TCTCTTCTTT TTTGGAGGAG
  601 TAGAATGTTG AGAGTCAGCA GTAGCCTCAT CATCACTAGA TGGCATTTCT TCTGAGCAAA
  661 ACAGGTTTTC CTCATTAAAG GCATTCCACC ACTGCTCCCA TTCATCAGTT CCATAGGTTG
  721 GAATCTAAAA TACACAAACA ATTAGAATCA GTAGTTTAAC ACATTATACA CTTAAAAATT
  781 TTATATTTAC CTTANAGCTT TAAATCTCTG TANGTAGTTT GTCCAATTAT GTCACACCAC
  841 AGAANTAANG TTCNTTCACA AAGNTCCGGC GAATTTCTGN CATTCATCCG CTTATTATCA
  901 CTTATTCAGG NGNANCAANN NGNNGTTNNA NNNNCACNNA NAACNGCNNT AAAAAAATTA
961 NNNCCCNGNC NNNNNNNNCA TCGNNNNANN NNNNNNNN
//
Primer: SV40-3'UTR ()
Region: Luciferase3'
Sequence file: RDB17469_A9Gpc.seq check
>06775_17469_A9Gp_SV40-3'UTR_A10_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNT NNNNNAGNNC CGGCNAANTT CTGCCNTTCN TCCGCTTATT
   61 ATCACTTATT CAGGCGTAGC AACCAGGCGT TTAAGGGCAC CAATAACTGC CTTAAAAAAA
  121 TTACGCCCCG CCCTGCCACT CATCGCAGTA TTACAATAGC TAAGAATTTC GTCATCGCTG
  181 AATACAGTTA CATTTTACAA TTTGGACTTT CCGCCCTTCT TGGCCTTTAT GAGGATCTCT
  241 CTGATTTTTC TTGCGTCGAG TTTTCCGGTA AGACCTTTCG GTACTTCGTC CACAAACACA
  301 ACTCCTCCGC GCAACTTTTT CGCGGTTGTT ACTTGACTGG CGACGTAATC CACGATCTCT
  361 TTTTCCGTCA TCGTCTTTCC GTGCTCCAAA ACAACAACGG CGGCGGGAAG TTCACCGGCG
  421 TCATCGTCGG GAAGACCTGC CACGCCCGCG TCGAAGATGT TGGGGTGTTG TAACAATATC
  481 GATTCCAATT CAGCGGGGGC CACCTGATAT CCTTTGTATT TAATTAAAGA CTTCAAGCGG
  541 TCAACTATGA AGAAGTGTTC GTCTTCGTCC CAGTAAGCTA TGTCTCCAGA ATGTAGCCAT
  601 CNATCCTTGT CAATCAAGGC GTTGGTCGCT TCCGGATTGT TTACATAACC GGACATAATC
  661 ATAGGTCCTC TGACACATAA TTCGCCTCTC TGATTAACGC CCAGCGTTTT CCCGGTATCC
  721 AGATCCACAA CCTTCGCTTC AAAAAATGGA ACAACTTTAC CGACCGCGCC CGGTTTATCA
  781 TCCCCCTCGG GTGTAATCAG AATAGCTGAT GTAGTCTCAG TGAGCNNATA TCCTTGTCGT
  841 ATCCCNGNNA GANGNAANCG TTTNGCAACC GCTTCCNNNA CTTCNTTCGA NNNNNNGNGN
  901 GCCCCNNNAA NCAATTNCNN GNNAATTAGA TAAATCNNAN TNNNNNANTC ANNNNNNNTT
961 TNNNNNNNNN N
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kato, H., Involvement of RBP-J in biological functions of mouse Notch1 and its derivatives. Development, 124 (20): 4133-4141 (1997). PMID 9374409. [link to RRC of NBRP]
user_report Saito, Y., Casitas B-cell lymphoma mutation in childhood T-cell acute lymphoblastic leukemia. Leuk Res., 36 (8): 1009-1015 (2012). PMID 22591685. [link to RRC of NBRP]