Resource data sheet
Prev.

pEF-BOSneoSE-mNotch2 RAMIC

Expression vector of mouse Notch2 intracellular region

Catalog number RDB06772
Resource name pEF-BOSneoSE-mNotch2 RAMIC
Alternative name #10035
Clone info. Expression vector of mouse Notch2 intracellular region.
Vector backbone pEF-BOS neo SE (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Notch gene homolog 2 cDNA
Depositor Honjo, Tasuku |
 
Other clones in our bank mouse Notch2 (NCBI Gene 18129) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by DEPOSITOR (FEBS Lett., 395, 221-224, 1996) and an acknowledgment to Depositor are requested. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.
Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06772 pEF-BOSneoSE-mNotch2 RAMIC DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Kato, H., Functional conservation of mouse Notch receptor family members. FEBS Lett., 395, 221-224 (1996). PMID 8898100.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12305_A3Jt.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxEFF1 RDB12305_A3Jta.seq
>06772_12305_A3Jt_pAxEFF1_A08_02.ab1
    1 CTTTTTTCGT CATTTCAGTG TCGTGAGGAA TTCTCTAGCC CAACACCATG GAGCAAAAGC
   61 TCATTTCTGA AGAGGACTTG AGAGAATTCA AGCGGAAGCG CAAGCATGGC TTCCTCTGGC
  121 TGCCGGAAGG GTTCACCCTC CGCCGAGACT CTAGCAATCA CAAGCGCCGT GAACCTGTGG
  181 GACAGGATGC CGTGGGGCTG AAAAATCTCT CCGTGCAAGT GTCAGAGGCT AACCTGATTG
  241 GTTCTGGGAC AAGTGAACAT TGGGTTGATG ATGAAGGACC CCAGCCAAAG AAAGCCAAGG
  301 CTGAGGATGA GGCTTTGCTG TCGGAAGATG ACCCCATCGA TCGACGGCCC TGGACACAGC
  361 AGCACCTTGA AGCTGCAGAC ATCCGCCACA CTCCATCCCT GGCACTCACT CCTCCTCAGG
  421 CAGAACAGGA GGTGGACGTG CTGGACGTGA ATGTCCGAGG CCCAGATGGG TGTACTCCAC
  481 TGATGCTGGC TTCTCTCCGA GGAGGCAGCT CAGACCTGAG TGATGAAGAC GAAGATGCTG
  541 AGGACTCTTC TGCCAACATC ATCACAGACT TGGTCTACCA AGGTGCCAGC CTTCAAGCAC
  601 AGACAGACCG CACTGGCGAG ATGGCCCTGC ACCTTGCAGC CCGCTATTCG AGAGCTGATG
661 CTGCCAAACG CCT
//
Primer: hGCSF-R RDB12305_A3Jtb.seq
>06772_12305_A3Jt_hGCSF-R_B08_05.ab1
    1 CGTGGACTGA TCTCTCAGGG TCCCAGGTGG GGACCCTCAC TCTAGGATAT CATCTAGACG
   61 GATCCGCAAT TGGCCCCAAC GGATCAGGTG ATAAATGTGC TTCACAGTGG CAGTCAATGG
  121 AGGGCCTGGA GGGGGGCGAT TGCTTCTTGA GCAAGTTAGC TTATTTCTTG GACATTCCAA
  181 CACTCACCCA CTTTTCCTAC TTTACAACAA AATGAGAGGA ATATCACAGT ACCTAAGCCT
  241 CCTAGAGTTC AATTTTATAC TGACTAGGCC AACTGACAGA AGAACAATTC AGATCCTTCC
  301 CCCTGATGAG TGTATATGGG GCCCCACATG CAGGGGAGAG AAATTAAGGT GCAGGTTCAG
  361 AAGGAGGCTT CTAGTCCCCA AATATTTTGC TTCCTCTTTC CTTCTAGGAA TAAGACATGA
  421 AGACTAGTGT GTGCATTTTC CATAGAAGCA TACTTCAGAG GCCAGGGCTG AGACCCATGG
  481 CCAGAGTAAA CCCTGTGAGG CACCTGTGAG GTGCCGGGAA TGTGGGAACA GGAGGCTGAA
  541 GTCGGTTTCC AGGTCAGGAA TGATTAAGAG CACAGACATC TCGCTTATGA CACACTGACG
  601 GGGATCAACC ACAGACGGGC GCAGAGAGGA AAGCACCGAG TGACGTCAAG GCCACTTCCA
661 GGGCTGAGAA GAC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Kato, H., Functional conservation of mouse Notch receptor family members. FEBS Lett., 395, 221-224 (1996). PMID 8898100.
user report Hayashi, Y., NOTCH2 signaling confers immature morphology and aggressiveness in human hepatocellular carcinoma cells. Oncol. Rep. 34, 1650-1658 (2015). PMID 26252838.
user report Ylivinkka, I., Netrin-1-induced activation of Notch signaling mediates glioblastoma cell invasion. J. Cell Sci. 126 (Pt 11): 2459-2469 (2013). PMID 23549787.

2019.03.08

GNP_filter3_RDBDEP_html_190307.pl