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pEF-BOSneoSE-mNotch2 RAMIC (#RDB06772)

Expression vector of mouse Notch2 intracellular region

Alternative name #10035
Clone info. Expression vector of mouse Notch2 intracellular region.
Vector backbone pEF-BOS neo SE (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Notch gene homolog 2 cDNA
Depositor|Developer Honjo, Tasuku |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by DEPOSITOR (FEBS Lett., 395, 221-224, 1996) and an acknowledgment to Depositor are requested. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。営利機関の利用者は、事前に京都大学・「医学領域」産学連携推進機構・知財室 (contracts@kumbl.med.kyoto-u.ac.jp) に連絡し、承諾を得ること。利用者は、研究成果の公表にあたって寄託者の指定する文献(FEBS Lett., 395, 221-224, 1996)を引用し、寄託者への謝辞の表明を必要とする。理研BRCは、本件リソースのリソース名、提供先、利用目的、件数を京都大学・本庶佑教授に報告する。
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06772 pEF-BOSneoSE-mNotch2 RAMIC DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-BOSneoSE-mNotch2 RAMIC was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06772).

Reference section:

Kato, H., Sakai, T., Tamura, K., Minoguchi, S., Shirayoshi, Y., Hamada, Y., Tsujimoto, Y., Honjo, T., Functional conservation of mouse Notch receptor family members. FEBS Lett., 395, 221-224 (1996). PMID 8898100. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18609_B0Jlp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168)
Region: EF-1 alpha pro,Myc,insert 5'
Sequence file: RDB18609_B0Jla.seq check
>06772_18609_B0Jl_1_EF1a_C_F_A11_02_ABI24.ab1
    1 NNNNNNNNNT TTTTGANTTT GGNNCTTGGT TCNTTCTCNA GCCTCAGACA GTGGTTCAAA
   61 GTTTTTTTCT TCCATTTCAG GTGTCGTGAG GAATTCTCTA GCCCAACACC ATGGAGCAAA
  121 AGCTCATTTC TGAAGAGGAC TTGAGAGAAT TCAAGCGGAA GCGCAAGCAT GGCTTCCTCT
  181 GGCTGCCGGA AGGGTTCACC CTCCGCCGAG ACTCTAGCAA TCACAAGCGC CGTGAACCTG
  241 TGGGACAGGA TGCCGTGGGG CTGAAAAATC TCTCCGTGCA AGTGTCAGAG GCTAACCTGA
  301 TTGGTTCTGG GACAAGTGAA CATTGGGTTG ATGATGAAGG ACCCCAGCCA AAGAAAGCCA
  361 AGGCTGAGGA TGAGGCTTTG CTGTCGGAAG ATGACCCCAT CGATCGACGG CCCTGGACAC
  421 AGCAGCACCT TGAAGCTGCA GACATCCGCC ACACTCCATC CCTGGCACTC ACTCCTCCTC
  481 AGGCAGAACA GGANGTGGAC GTGCTGGACG TGAATGTCCG AGGCCCAGAT GGGTGTACTC
  541 CACTGATGCT GGCTTCTCTC CGAGGANGCA GCTCAGANCT GAGTGANGAA GANGAAGANG
  601 CTGANGACTC TTCTGCCNAC ATCATCACAG ACTTGGTCTA CCNAGGTGCC NGCCTTCNAG
661 CACAGACAGA NCNCACNGGC GANNNGGNCC NGCNNNNNNN NNCCNNNNNN NNNN
//
Primer: hGCSF_R (Pr0031)
Region: hGCSF-3' UTR+pA,insert 3'
Sequence file: RDB18609_B0Jlb.seq check
>06772_18609_B0Jl_1_hGCSF_R_B11_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN GTCCNNGGNG GGGNNCCTCA CTCTAGGATA TCATCTAGAC
   61 GGATCCGCAA TTGGCCCCAA CGGATCAGGT GATAAATGTG CTTCACAGTG GCAGTCAATG
  121 GAGGGCCTGG AGGGGGGCGA TTGCTTCTTG AGCAAGTTAG CTTATTTCTT GGACATTCCA
  181 ACACTCACCC ACTTTTCCTA CTTTACAACA AAATGAGAGG AATATCACAG TACCTAAGCC
  241 TCCTAGAGTT CAATTTTATA CTGACTAGGC CAACTGACAG AAGAACAATT CAGATCCTTC
  301 CCCCTGATGA GTGTATATGG GGCCCCACAT GCAGGGGAGA GAAATTAAGG TGCAGGTTCA
  361 GAAGGAGGCT TCTAGTCCCC AAATATTTTG CTTCCTCTTT CCTTCTAGGA ATAAGACATG
  421 AAGACTAGTG TGTGCATTTT CCATAGAAGC ATACTTCAGA GGCCAGGGCT GAGACCCATG
  481 GCCAGAGTAA ACCCTGTGAG GCACCTGTGA GGTGCCGGGA ATGTGGGAAC AGGAGGCTGA
  541 AGTCGGTTTC CAGGTCAGGA ATGATTAAGA GCACAGACAT CTCGCTTATG ACACACTGAC
  601 GGGGNNCAAC CNCAGACGGG CGCAGANAGG AAAGCACCGA GTGACGTCNA GGNCACTTCN
661 NGGGNTGANA ANANTGGANA CGTCANCCNN NNNNNNNN
//
Primer: Neo-M_R (Pr0145)
Region: NeoR,TK pro
Sequence file: RDB18609_B0Jlc.seq check
>06772_18609_B0Jl_1_Neo-M_R_E11_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNC NNTTNNNNNN TNNGTGANNA CGTCGAGCAC AGCTGCGCAA
   61 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCCTGCAG TTCATTCAGG
  121 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC
  181 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC
  241 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATGGCCGA TCCCATATTG
  301 GCTGCAGGGT CGCTCGGTGT TCGAGGCCAC ACGCGTCACC TTAATATGCG AAGTGGACCT
  361 GGGACCGCGC CGCCCCGACT GCATCTGCGT GTTCGAATTC GCCAATGACA AGACGCTGGG
  421 CGGGGTTTGC TCGACATTGG GTGGAAACAT TCCAGGCCTG GGTGGAGAGG CTTTTTGCTT
  481 CCTCTTGCAA AACCACACTG CTCGACATTG GGTGGAAACA TTCCAGGCCT GGGTGGAGAG
  541 GCTTTTTGCT TCCTCTTGAA AACCACACTG CTCGACTGGC GAAAGGGGGA NGTGCTGNNN
  601 NGCGATTAAN TTGGGTAACG CCNNGGTTTT CCCAGTCNCG ANNTTNTAAA ACNNNNNNNA
661 NNGAANNNNN
//
Primer: notchi2-M_R (Pr0915)
Region: insert mid
Sequence file: RDB18609_B0Jld.seq check
>06772_18609_B0Jl_1_notch2-M_R_G01_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NTNNCGCTNG CCTCAGGTTT GGAGCCTCTC TCTCCTGCCT
   61 CCGAAAGATG GTCACGGACG GGNCGTCTCT TCATTTCTCT CCGGATAACC TTCACTCGCC
  121 CCTCAGCAGT ATGTGTGATA GGCACTTCCA TCTCTAAGGC TGAGGCAGAC TCTTCATGCA
  181 TACACCTGCA TGTTGCTGTG TGGTGGCTCG GACATGTGTG TTCCGGGTCC CCGCTGACCG
  241 CCTCCACCAC CTCCAGGAGT TGGGCTGGTG GTCACATCTG ACCAGTCAGA TGCAGAGTGT
  301 GGTGAAGAGC TTGACCATTG GTCAGGAGAC TCTGGGGATG GTGTCAGGTA TGGGTGCTCG
  361 CCCTGGAGGT GACCACCATG ACTGGGGGTT CGCTCAGCAG CATTTGAGGA GGCGTAACTG
  421 TGTTGGGAAG GGGGTGTGGG GTACTTGCCC ACAGAGGCTG GGAAAGGATG ATAGGTTGGC
  481 ACAATGGTCT GAGCTACCTG CCCCTCCTGC TGGGGCATCA TGGTAGGTGG GAAAGCCACA
  541 CTGGGCAAAC GGGGCATCTC TGGGATCTGG TACATAGAGG GCAAGGGGCC TGCAACAGCT
  601 GGAGGGCAAC TGGACTGCGT CTGGNNNGCT CCNNCTGCCT GGGCAATGCT GCCTTTTGGG
  661 NNAANCTGGA AAGTCNCGAT GGNNNNNNGG GCTCCNGCTG GGNNNNNNTG TGCTTCCCNN
721 NCNNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kato, H., Functional conservation of mouse Notch receptor family members. FEBS Lett., 395, 221-224 (1996). PMID 8898100. [link to RRC of NBRP]
user_report Hayashi, Y., NOTCH2 signaling confers immature morphology and aggressiveness in human hepatocellular carcinoma cells. Oncol. Rep. 34, 1650-1658 (2015). PMID 26252838. [link to RRC of NBRP]
user_report Ylivinkka, I., Netrin-1-induced activation of Notch signaling mediates glioblastoma cell invasion. J. Cell Sci. 126 (Pt 11): 2459-2469 (2013). PMID 23549787. [link to RRC of NBRP]