RDB06772 - pEF-BOSneoSE-mNotch2 RAMIC

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by DEPOSITOR (FEBS Lett., 395, 221-224, 1996) and an acknowledgment to Depositor are requested. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.
Additional terms and conditions for distribution	Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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提供案内 (日本国内)

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提供条件	本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。営利機関の利用者は、事前に京都大学・「医学領域」産学連携推進機構・知財室 (contracts@kumbl.med.kyoto-u.ac.jp) に連絡し、承諾を得ること。利用者は、研究成果の公表にあたって寄託者の指定する文献(FEBS Lett., 395, 221-224, 1996)を引用し、寄託者への謝辞の表明を必要とする。理研BRCは、本件リソースのリソース名、提供先、利用目的、件数を京都大学・本庶佑教授に報告する。
付加的提供条件	pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

References and tips

Sequence information

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB06772	pEF-BOSneoSE-mNotch2 RAMIC	DNA solution

original	Kato, H., Functional conservation of mouse Notch receptor family members. FEBS Lett., 395, 221-224 (1996). PMID 8898100.

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

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original	Kato, H., Functional conservation of mouse Notch receptor family members. FEBS Lett., 395, 221-224 (1996). PMID 8898100.
user report	Hayashi, Y., NOTCH2 signaling confers immature morphology and aggressiveness in human hepatocellular carcinoma cells. Oncol. Rep. 34, 1650-1658 (2015). PMID 26252838.
user report	Ylivinkka, I., Netrin-1-induced activation of Notch signaling mediates glioblastoma cell invasion. J. Cell Sci. 126 (Pt 11): 2459-2469 (2013). PMID 23549787.

Primer: EF1a-C_F Region: EF-1 alpha pro,Myc,insert 5' Sequence file: RDB18609_B0Jla.seq
>06772_18609_B0Jl_1_EF1a_C_F_A11_02_ABI24.ab1 1 NNNNNNNNNT TTTTGANTTT GGNNCTTGGT TCNTTCTCNA GCCTCAGACA GTGGTTCAAA 61 GTTTTTTTCT TCCATTTCAG GTGTCGTGAG GAATTCTCTA GCCCAACACC ATGGAGCAAA 121 AGCTCATTTC TGAAGAGGAC TTGAGAGAAT TCAAGCGGAA GCGCAAGCAT GGCTTCCTCT 181 GGCTGCCGGA AGGGTTCACC CTCCGCCGAG ACTCTAGCAA TCACAAGCGC CGTGAACCTG 241 TGGGACAGGA TGCCGTGGGG CTGAAAAATC TCTCCGTGCA AGTGTCAGAG GCTAACCTGA 301 TTGGTTCTGG GACAAGTGAA CATTGGGTTG ATGATGAAGG ACCCCAGCCA AAGAAAGCCA 361 AGGCTGAGGA TGAGGCTTTG CTGTCGGAAG ATGACCCCAT CGATCGACGG CCCTGGACAC 421 AGCAGCACCT TGAAGCTGCA GACATCCGCC ACACTCCATC CCTGGCACTC ACTCCTCCTC 481 AGGCAGAACA GGANGTGGAC GTGCTGGACG TGAATGTCCG AGGCCCAGAT GGGTGTACTC 541 CACTGATGCT GGCTTCTCTC CGAGGANGCA GCTCAGANCT GAGTGANGAA GANGAAGANG 601 CTGANGACTC TTCTGCCNAC ATCATCACAG ACTTGGTCTA CCNAGGTGCC NGCCTTCNAG 661 CACAGACAGA NCNCACNGGC GANNNGGNCC NGCNNNNNNN NNCCNNNNNN NNNN //
Primer: hGCSF_R Region: hGCSF-3' UTR+pA,insert 3' Sequence file: RDB18609_B0Jlb.seq
>06772_18609_B0Jl_1_hGCSF_R_B11_05_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN GTCCNNGGNG GGGNNCCTCA CTCTAGGATA TCATCTAGAC 61 GGATCCGCAA TTGGCCCCAA CGGATCAGGT GATAAATGTG CTTCACAGTG GCAGTCAATG 121 GAGGGCCTGG AGGGGGGCGA TTGCTTCTTG AGCAAGTTAG CTTATTTCTT GGACATTCCA 181 ACACTCACCC ACTTTTCCTA CTTTACAACA AAATGAGAGG AATATCACAG TACCTAAGCC 241 TCCTAGAGTT CAATTTTATA CTGACTAGGC CAACTGACAG AAGAACAATT CAGATCCTTC 301 CCCCTGATGA GTGTATATGG GGCCCCACAT GCAGGGGAGA GAAATTAAGG TGCAGGTTCA 361 GAAGGAGGCT TCTAGTCCCC AAATATTTTG CTTCCTCTTT CCTTCTAGGA ATAAGACATG 421 AAGACTAGTG TGTGCATTTT CCATAGAAGC ATACTTCAGA GGCCAGGGCT GAGACCCATG 481 GCCAGAGTAA ACCCTGTGAG GCACCTGTGA GGTGCCGGGA ATGTGGGAAC AGGAGGCTGA 541 AGTCGGTTTC CAGGTCAGGA ATGATTAAGA GCACAGACAT CTCGCTTATG ACACACTGAC 601 GGGGNNCAAC CNCAGACGGG CGCAGANAGG AAAGCACCGA GTGACGTCNA GGNCACTTCN 661 NGGGNTGANA ANANTGGANA CGTCANCCNN NNNNNNNN //
Primer: Neo-M_R Region: NeoR,TK pro Sequence file: RDB18609_B0Jlc.seq
>06772_18609_B0Jl_1_Neo-M_R_E11_14_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNC NNTTNNNNNN TNNGTGANNA CGTCGAGCAC AGCTGCGCAA 61 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCCTGCAG TTCATTCAGG 121 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 181 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 241 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATGGCCGA TCCCATATTG 301 GCTGCAGGGT CGCTCGGTGT TCGAGGCCAC ACGCGTCACC TTAATATGCG AAGTGGACCT 361 GGGACCGCGC CGCCCCGACT GCATCTGCGT GTTCGAATTC GCCAATGACA AGACGCTGGG 421 CGGGGTTTGC TCGACATTGG GTGGAAACAT TCCAGGCCTG GGTGGAGAGG CTTTTTGCTT 481 CCTCTTGCAA AACCACACTG CTCGACATTG GGTGGAAACA TTCCAGGCCT GGGTGGAGAG 541 GCTTTTTGCT TCCTCTTGAA AACCACACTG CTCGACTGGC GAAAGGGGGA NGTGCTGNNN 601 NGCGATTAAN TTGGGTAACG CCNNGGTTTT CCCAGTCNCG ANNTTNTAAA ACNNNNNNNA 661 NNGAANNNNN //
Primer: notchi2-M_R Region: insert mid Sequence file: RDB18609_B0Jld.seq
>06772_18609_B0Jl_1_notch2-M_R_G01_07_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NTNNCGCTNG CCTCAGGTTT GGAGCCTCTC TCTCCTGCCT 61 CCGAAAGATG GTCACGGACG GGNCGTCTCT TCATTTCTCT CCGGATAACC TTCACTCGCC 121 CCTCAGCAGT ATGTGTGATA GGCACTTCCA TCTCTAAGGC TGAGGCAGAC TCTTCATGCA 181 TACACCTGCA TGTTGCTGTG TGGTGGCTCG GACATGTGTG TTCCGGGTCC CCGCTGACCG 241 CCTCCACCAC CTCCAGGAGT TGGGCTGGTG GTCACATCTG ACCAGTCAGA TGCAGAGTGT 301 GGTGAAGAGC TTGACCATTG GTCAGGAGAC TCTGGGGATG GTGTCAGGTA TGGGTGCTCG 361 CCCTGGAGGT GACCACCATG ACTGGGGGTT CGCTCAGCAG CATTTGAGGA GGCGTAACTG 421 TGTTGGGAAG GGGGTGTGGG GTACTTGCCC ACAGAGGCTG GGAAAGGATG ATAGGTTGGC 481 ACAATGGTCT GAGCTACCTG CCCCTCCTGC TGGGGCATCA TGGTAGGTGG GAAAGCCACA 541 CTGGGCAAAC GGGGCATCTC TGGGATCTGG TACATAGAGG GCAAGGGGCC TGCAACAGCT 601 GGAGGGCAAC TGGACTGCGT CTGGNNNGCT CCNNCTGCCT GGGCAATGCT GCCTTTTGGG 661 NNAANCTGGA AAGTCNCGAT GGNNNNNNGG GCTCCNGCTG GGNNNNNNTG TGCTTCCCNN 721 NCNNNNNNNN NN //

pEF-BOSneoSE-mNotch2 RAMIC

Distribution information

References and tips

Sequence information

References