Expression vector of mouse Notch1 intracellular region
Alternative name | #12249 |
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Clone info. | Expression vector of mouse Notch1 intracellular region. RAMIC is tagged with 6x Myc epitope sequence. |
Comment | Insertion of 12 amino acids sequence of myc-tag (SEQKLISEEDlS) was identified between 2,293 and 2,294aa corresponding Fig.1 of Kato, H., FEBS Lett. 395: 221-224, 1996 (Feb.17, 2017). |
Vector backbone | pEF-BOS neo (Plasmid) |
Selectable markers | Amp^r |
Gene/insert name | Mouse Notch gene homolog 1 cDNA |
Depositor|Developer | Honjo, Tasuku | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by DEPOSITOR (FEBS Lett., 395, 221-224, 1996) and an acknowledgment to Depositor are requested. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources. Additional terms and conditions: Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。営利機関の利用者は、事前に京都大学・「医学領域」産学連携推進機構・知財室 (contracts@kumbl.med.kyoto-u.ac.jp) に連絡し、承諾を得ること。利用者は、研究成果の公表にあたって寄託者の指定する文献(FEBS Lett., 395, 221-224, 1996)を引用し、寄託者への謝辞の表明を必要とする。理研BRCは、本件リソースのリソース名、提供先、利用目的、件数を京都大学・本庶佑教授に報告する MTAに書く付加的使用条件: pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB06771 | pEF-BOSneo-mNotch1 RAMIC | DNA solution |
Materials & Methods section:
The pEF-BOSneo-mNotch1 RAMIC was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06771). |
Reference section:
Kato, H., Sakai, T., Tamura, K., Minoguchi, S., Shirayoshi, Y., Hamada, Y., Tsujimoto, Y., Honjo, T., Functional conservation of mouse Notch receptor family members. FEBS Lett., 395, 221-224 (1996). PMID 8898100. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB18622_B0Jlp1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: EF1a-C_F (Pr0168) Region: EF-1 alpha pro,Myc x6,insert 5' Sequence file: RDB18622_B0Jla.seq |
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>06771_18622_B0Jl_1_EF1a-C_F_F09_06_ABI08.ab1 1 NNNNNNNNNN NNNNNNNTTT NNANTTTGGN NCTTGGTTCN TTCTCNAGCC TCAGACAGTG 61 GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGAA TTCTCTGACG GTATCGATTT 121 AAAGCTATGG AGCAAAAGCT CATTTCTGAA GAGGACTTGA ATGAAATGGA GCAAAAGCTC 181 ATTTCTGAAG AGGACTTGAA TGAAATGGAG CAAAAGCTCA TTTCTGAAGA GGACTTGAAT 241 GAAATGGAGC AAAAGCTCAT TTCTGAAGAG GACTTGAATG AAATGGAGCA AAAGCTCATT 301 TCTGAAGAGG ACTTGAATGA AATGGAGAGC TTGGGCGACC TCACCATGGA GCAAAAGCTC 361 ATTTCTGAAG AGGACTTGAA TTCCCGCAAG CGCCGGCGGC AGCATGGCCA GCTCTGGTTC 421 CCTGAGGGTT TCAAAGTGTC AGAGGCCAGC AAGAAGAAGC GGAGAGAGCC CCTCGGCGAG 481 GACTCAGTCG GCCTCAAGCC CCTGAAGAAT GCCTCAGATG GTGCTCTGAT GGACGACAAT 541 CAGAACGAGT GGGGAGACGA AGACCTGGAG ACCAAGAAGT TCCGGTTTGA GGAGCCAGTA 601 GTTCTCCCTG ACCTGAGTGA TCAGACTGAC CACAGGCAGT GGACCCAGCA GCACCTGGAC 661 GCTGCTGACC TGCGCATGTC TGCCATGGCC CCAACACCGC CTCAGGGGGA GGTGGATGCT 721 GACTGCATGG ATGTCAATGT TCGAGGACCA GATGGCTTCA CACCCCTCAT GATTGCCTCC 781 TGCAGTGNAN GGGGCCTTGA GACANGCAAC AGTGAANAAN AANAAGANGC ACCTGCTGNC 841 ATCTCTGANN NCNTCTACNN NGGCGNCAGC TTGCACAACC AGANAGACCG CNNCNGGGGA 901 GANCGNCTNN CACTTGNCTG CCNNANACTC TCNTTCANAN GCTNNNAANC GCTNGNTNGN 961 NNGNNANNGN NNNNNNNNN // |
Primer: RDB06771#1 (Pr0422) Region: insert mid(insert -Myc-insert ) Sequence file: RDB18622_B0Jlb.seq |
>06771_18622_B0Jl_1_RDB06771#1_H01_08_ABI08.ab1 1 NNNNNNNNNN NNNNNNGGNG TGCNNAGGCA GANTGCTGGG GCCCAGCGGT TGTACATCTG 61 CCTGACTGGG CTCCCCACTC AAGAAGCTCC GGCCCAGGTG CCCATTGGCT GCCGAGCTCA 121 CACTGAGGTG TGGCTGTGAT GGTGGCTGCA GGTTCTGAGG CTGGAGCTGT AAGTTCTGTG 181 GCTGCACCTG CTGGGTCTGC ACCAGGTGAG GCTGTGTTGC CAGCCGTGTG TTGGGCAGGC 241 CCTGGTAAAT AATCGGGGAC AAGGTATTGG TGGAGAGGCT GCTGTGTAGT GGCCCCATCA 301 TGCTATGCTG GAGGCCAGCT GCCTGTGTGC TCAGTGTGCC CGGCGTCACA CCCGGCCGTA 361 GTGGGTTGTA CTGGCTGGGC ACCATGCCAT TCTGGAGCCG GGGAAGCCAC TCACACTGGC 421 CATTCAAGCT TAGGTCCTCT TCAGAAATGA GCTTTTGCTC GGAGCTTGCC GGTGCACCCA 481 CGGTGAAATT CATAGCCCCC GTGCCATTGG TACTCAGCAC TGTGCTGGCA CTGGAGGCTA 541 CAGGCAGGTG GGAGAGGCGT GGCGGGGGTG GCTCANAGGC CAACCGGCTA CCTCCAGCCA 601 GTGCTGCCAT CTCANGCTTG GCTGCCACAT TNNAGTGGCT GANGCCCAGG TGANTGTCNN 661 NCATACCANG CNNNGGCTGA NANGNATNNN NGGANACTGC TNNNNNGGGG ANGGNNNNNN 721 NGGGNNNNNN NNNN // |
Primer: hGCSF_R (Pr0031) Region: hGCSF-3'UTR+pA,insert 3' Sequence file: RDB18622_B0Jlc.seq |
>06771_18622_B0Jl_1_hGCSF_R_H01_08_ABI08.ab1 1 NNNNNNNNNN NCNNNNNGTC CNNGGNGGGG NNCCTCACTC TACTAGAACT AGTGGATCCC 61 CAATTCATGT CTTACGGTCA AGGTTTTTTT TTTTCGATTT TGGAAAGAAG CAATCAATAA 121 ATAAACAGCA TTTACAAATC ATGAATCCTT GTTCATATTT TACAGACACA CAGGGAACTT 181 CACCCTGACC AGGAAAATCA AGGCTCTGAG AACTAGTTTA TTTTCTTGGA ACAGATATGT 241 TTTTCCCTAG TGCCATAGAA AACAATACAT ATAAATAAAA AGGCAGTGTT TCTGTGGAAA 301 ATAAAAGTAC ATAAATAAAT ACTAAAAAAA AAAATGGAAA TTCTTGTCCT CTTATTTTGT 361 ATAAAAACAT TTTTTAAAAG GCTCCTTTGG TCGCCCCAGC ATCCTGAAGC ACTGGAAAGG 421 ACTCCCAACA GGGCTTGGGA ACGGAAGCTG GGGTCCTGCA TCCCACATCT CTGTTTATTT 481 AAATGCCTCT GGAATGTGGG TGATCTGGGA CGGCATGGTG GTGGGCGGGC TGGAGATGCC 541 CTCGGACCAA TCAGAGATGT TGGAATGCGG GGAGGAGCTG GACCACTGGT CAGGGGACTC 601 AGGGGANGGG GTGAGGAAGG NGTGCTCTGG CACCTGCAGC TGGTGGCTGG GGGTGTTGTC 661 CACAGGGGAN GNNNAGTNNC TGNGCTGGNN ANGANGGGTC NNNNNCTGGN NANNNNNNNT 721 GGNNNGNNNN NNNNNN // |
Primer: Neo-M_R (Pr0145) Region: NeoR, TK pro Sequence file: RDB18622_B0Jld.seq |
>06771_18622_B0Jl_1_Neo-M_R_H09_08_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTTC NNNNCTTNNG TGNCNACNNT CGAGCACAGC 61 TGCGCAAGGA ACGCCCGTCG TGGCCNGCCA CGATAGCCGC GCTGCCTCGT CCTGCAGTTC 121 ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG 181 CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG 241 CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TGGCCGATCC 301 CATATTGGCT GCAGGGTCGC TCGGTGTTCG AGGCCACACG CGTCACCTTA ATATGCGAAG 361 TGGACCTGGG ACCGCGCCGC CCCGACTGCA TCTGCGTGTT CGAATTCGCC AATGACAAGA 421 CGCTGGGCGG GGTTTGCTCG ACATTGGGTG GAAACATTCC AGGCCTGGGT GGAGAGGCTT 481 TTTGCTTCCT CTTGCAAAAC CACACTGCTC GACATTGGGT GGAAACATTC CAGGCCTGGG 541 TGGAGAGGCT TTTTGCTTCC TCTTGAAAAC CACACTGCTC GACTGGCGAA AGGGGGATGT 601 GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG 661 ACGGCCAGTG AATTCGNNNT TTNNTTNNNN NNNNNTNNNN NNNTTNNNNN NNNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Kato, H., Functional conservation of mouse Notch receptor family members. FEBS Lett., 395, 221-224 (1996). PMID 8898100. [link to RRC of NBRP] |
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user_report | Yoneyama, T., Notch Inhibitors from Calotropis gigantea That Induce Neuronal Differentiation of Neural Stem Cells. J. Nat. Prod. 80 (9): 2453-2461 (2017). PMID 28817274. [link to RRC of NBRP] |
user_report | Kitajima, K., Wnt signaling regulates left-right axis formation in the node of mouse embryos. Dev. Biol., 380 (2): 222-232 (2013). PMID 23707899. [link to RRC of NBRP] |
user_report | Saito, Y., Casitas B-cell lymphoma mutation in childhood T-cell acute lymphoblastic leukemia. Leuk Res., 36 (8): 1009-1015 (2012). PMID 22591685. [link to RRC of NBRP] |
user_report | O'Brien, L.L., Wt1a, Foxc1a, and the Notch mediator Rbpj physically interact and regulate the formation of podocytes in zebrafish. Dev. Biol., 358 (2): 318-330 (2011). PMID 21871448. [link to RRC of NBRP] |
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