Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEF-BOSneo-mNotch1 RAMIC

Expression vector of mouse Notch1 intracellular region

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by DEPOSITOR (FEBS Lett., 395, 221-224, 1996) and an acknowledgment to Depositor are requested. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources. Additional terms and conditions: Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.

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提供条件

本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。営利機関の利用者は、事前に京都大学・「医学領域」産学連携推進機構・知財室 (contracts@kumbl.med.kyoto-u.ac.jp) に連絡し、承諾を得ること。利用者は、研究成果の公表にあたって寄託者の指定する文献(FEBS Lett., 395, 221-224, 1996)を引用し、寄託者への謝辞の表明を必要とする。理研BRCは、本件リソースのリソース名、提供先、利用目的、件数を京都大学・本庶佑教授に報告する 付加的提供条件： pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

提供依頼

提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18622_B0Jlp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: EF-1 alpha pro,Myc x6,insert 5' Sequence file: RDB18622_B0Jla.seq
>06771_18622_B0Jl_1_EF1a-C_F_F09_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNTTT NNANTTTGGN NCTTGGTTCN TTCTCNAGCC TCAGACAGTG    61 GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGAA TTCTCTGACG GTATCGATTT   121 AAAGCTATGG AGCAAAAGCT CATTTCTGAA GAGGACTTGA ATGAAATGGA GCAAAAGCTC   181 ATTTCTGAAG AGGACTTGAA TGAAATGGAG CAAAAGCTCA TTTCTGAAGA GGACTTGAAT   241 GAAATGGAGC AAAAGCTCAT TTCTGAAGAG GACTTGAATG AAATGGAGCA AAAGCTCATT   301 TCTGAAGAGG ACTTGAATGA AATGGAGAGC TTGGGCGACC TCACCATGGA GCAAAAGCTC   361 ATTTCTGAAG AGGACTTGAA TTCCCGCAAG CGCCGGCGGC AGCATGGCCA GCTCTGGTTC   421 CCTGAGGGTT TCAAAGTGTC AGAGGCCAGC AAGAAGAAGC GGAGAGAGCC CCTCGGCGAG   481 GACTCAGTCG GCCTCAAGCC CCTGAAGAAT GCCTCAGATG GTGCTCTGAT GGACGACAAT   541 CAGAACGAGT GGGGAGACGA AGACCTGGAG ACCAAGAAGT TCCGGTTTGA GGAGCCAGTA   601 GTTCTCCCTG ACCTGAGTGA TCAGACTGAC CACAGGCAGT GGACCCAGCA GCACCTGGAC   661 GCTGCTGACC TGCGCATGTC TGCCATGGCC CCAACACCGC CTCAGGGGGA GGTGGATGCT   721 GACTGCATGG ATGTCAATGT TCGAGGACCA GATGGCTTCA CACCCCTCAT GATTGCCTCC   781 TGCAGTGNAN GGGGCCTTGA GACANGCAAC AGTGAANAAN AANAAGANGC ACCTGCTGNC   841 ATCTCTGANN NCNTCTACNN NGGCGNCAGC TTGCACAACC AGANAGACCG CNNCNGGGGA   901 GANCGNCTNN CACTTGNCTG CCNNANACTC TCNTTCANAN GCTNNNAANC GCTNGNTNGN 961 NNGNNANNGN NNNNNNNNN //
Primer: RDB06771#1 (Pr0422) Region: insert mid(insert -Myc-insert ) Sequence file: RDB18622_B0Jlb.seq
>06771_18622_B0Jl_1_RDB06771#1_H01_08_ABI08.ab1     1 NNNNNNNNNN NNNNNNGGNG TGCNNAGGCA GANTGCTGGG GCCCAGCGGT TGTACATCTG    61 CCTGACTGGG CTCCCCACTC AAGAAGCTCC GGCCCAGGTG CCCATTGGCT GCCGAGCTCA   121 CACTGAGGTG TGGCTGTGAT GGTGGCTGCA GGTTCTGAGG CTGGAGCTGT AAGTTCTGTG   181 GCTGCACCTG CTGGGTCTGC ACCAGGTGAG GCTGTGTTGC CAGCCGTGTG TTGGGCAGGC   241 CCTGGTAAAT AATCGGGGAC AAGGTATTGG TGGAGAGGCT GCTGTGTAGT GGCCCCATCA   301 TGCTATGCTG GAGGCCAGCT GCCTGTGTGC TCAGTGTGCC CGGCGTCACA CCCGGCCGTA   361 GTGGGTTGTA CTGGCTGGGC ACCATGCCAT TCTGGAGCCG GGGAAGCCAC TCACACTGGC   421 CATTCAAGCT TAGGTCCTCT TCAGAAATGA GCTTTTGCTC GGAGCTTGCC GGTGCACCCA   481 CGGTGAAATT CATAGCCCCC GTGCCATTGG TACTCAGCAC TGTGCTGGCA CTGGAGGCTA   541 CAGGCAGGTG GGAGAGGCGT GGCGGGGGTG GCTCANAGGC CAACCGGCTA CCTCCAGCCA   601 GTGCTGCCAT CTCANGCTTG GCTGCCACAT TNNAGTGGCT GANGCCCAGG TGANTGTCNN   661 NCATACCANG CNNNGGCTGA NANGNATNNN NGGANACTGC TNNNNNGGGG ANGGNNNNNN 721 NGGGNNNNNN NNNN //
Primer: hGCSF_R (Pr0031) Region: hGCSF-3'UTR+pA,insert 3' Sequence file: RDB18622_B0Jlc.seq
>06771_18622_B0Jl_1_hGCSF_R_H01_08_ABI08.ab1     1 NNNNNNNNNN NCNNNNNGTC CNNGGNGGGG NNCCTCACTC TACTAGAACT AGTGGATCCC    61 CAATTCATGT CTTACGGTCA AGGTTTTTTT TTTTCGATTT TGGAAAGAAG CAATCAATAA   121 ATAAACAGCA TTTACAAATC ATGAATCCTT GTTCATATTT TACAGACACA CAGGGAACTT   181 CACCCTGACC AGGAAAATCA AGGCTCTGAG AACTAGTTTA TTTTCTTGGA ACAGATATGT   241 TTTTCCCTAG TGCCATAGAA AACAATACAT ATAAATAAAA AGGCAGTGTT TCTGTGGAAA   301 ATAAAAGTAC ATAAATAAAT ACTAAAAAAA AAAATGGAAA TTCTTGTCCT CTTATTTTGT   361 ATAAAAACAT TTTTTAAAAG GCTCCTTTGG TCGCCCCAGC ATCCTGAAGC ACTGGAAAGG   421 ACTCCCAACA GGGCTTGGGA ACGGAAGCTG GGGTCCTGCA TCCCACATCT CTGTTTATTT   481 AAATGCCTCT GGAATGTGGG TGATCTGGGA CGGCATGGTG GTGGGCGGGC TGGAGATGCC   541 CTCGGACCAA TCAGAGATGT TGGAATGCGG GGAGGAGCTG GACCACTGGT CAGGGGACTC   601 AGGGGANGGG GTGAGGAAGG NGTGCTCTGG CACCTGCAGC TGGTGGCTGG GGGTGTTGTC   661 CACAGGGGAN GNNNAGTNNC TGNGCTGGNN ANGANGGGTC NNNNNCTGGN NANNNNNNNT 721 GGNNNGNNNN NNNNNN //
Primer: Neo-M_R (Pr0145) Region: NeoR, TK pro Sequence file: RDB18622_B0Jld.seq
>06771_18622_B0Jl_1_Neo-M_R_H09_08_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTTC NNNNCTTNNG TGNCNACNNT CGAGCACAGC    61 TGCGCAAGGA ACGCCCGTCG TGGCCNGCCA CGATAGCCGC GCTGCCTCGT CCTGCAGTTC   121 ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG   181 CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG   241 CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TGGCCGATCC   301 CATATTGGCT GCAGGGTCGC TCGGTGTTCG AGGCCACACG CGTCACCTTA ATATGCGAAG   361 TGGACCTGGG ACCGCGCCGC CCCGACTGCA TCTGCGTGTT CGAATTCGCC AATGACAAGA   421 CGCTGGGCGG GGTTTGCTCG ACATTGGGTG GAAACATTCC AGGCCTGGGT GGAGAGGCTT   481 TTTGCTTCCT CTTGCAAAAC CACACTGCTC GACATTGGGT GGAAACATTC CAGGCCTGGG   541 TGGAGAGGCT TTTTGCTTCC TCTTGAAAAC CACACTGCTC GACTGGCGAA AGGGGGATGT   601 GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG 661 ACGGCCAGTG AATTCGNNNT TTNNTTNNNN NNNNNTNNNN NNNTTNNNNN NNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.10.05

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