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pEF-BOSneo-mNotch1 RAMIC (#RDB06771)

Expression vector of mouse Notch1 intracellular region

Alternative name #12249
Clone info. Expression vector of mouse Notch1 intracellular region. RAMIC is tagged with 6x Myc epitope sequence.
Comment Insertion of 12 amino acids sequence of myc-tag (SEQKLISEEDlS) was identified between 2,293 and 2,294aa corresponding Fig.1 of Kato, H., FEBS Lett. 395: 221-224, 1996 (Feb.17, 2017).
Vector backbone pEF-BOS neo (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Notch gene homolog 1 cDNA
Depositor|Developer Honjo, Tasuku |
 

Distribution information

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Terms and conditions for distribution RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by DEPOSITOR (FEBS Lett., 395, 221-224, 1996) and an acknowledgment to Depositor are requested. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
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手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。営利機関の利用者は、事前に京都大学・「医学領域」産学連携推進機構・知財室 (contracts@kumbl.med.kyoto-u.ac.jp) に連絡し、承諾を得ること。利用者は、研究成果の公表にあたって寄託者の指定する文献(FEBS Lett., 395, 221-224, 1996)を引用し、寄託者への謝辞の表明を必要とする。理研BRCは、本件リソースのリソース名、提供先、利用目的、件数を京都大学・本庶佑教授に報告する
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06771 pEF-BOSneo-mNotch1 RAMIC DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-BOSneo-mNotch1 RAMIC was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06771).

Reference section:

Kato, H., Sakai, T., Tamura, K., Minoguchi, S., Shirayoshi, Y., Hamada, Y., Tsujimoto, Y., Honjo, T., Functional conservation of mouse Notch receptor family members. FEBS Lett., 395, 221-224 (1996). PMID 8898100. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18622_B0Jlp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168)
Region: EF-1 alpha pro,Myc x6,insert 5'
Sequence file: RDB18622_B0Jla.seq check
>06771_18622_B0Jl_1_EF1a-C_F_F09_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNTTT NNANTTTGGN NCTTGGTTCN TTCTCNAGCC TCAGACAGTG
   61 GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGAA TTCTCTGACG GTATCGATTT
  121 AAAGCTATGG AGCAAAAGCT CATTTCTGAA GAGGACTTGA ATGAAATGGA GCAAAAGCTC
  181 ATTTCTGAAG AGGACTTGAA TGAAATGGAG CAAAAGCTCA TTTCTGAAGA GGACTTGAAT
  241 GAAATGGAGC AAAAGCTCAT TTCTGAAGAG GACTTGAATG AAATGGAGCA AAAGCTCATT
  301 TCTGAAGAGG ACTTGAATGA AATGGAGAGC TTGGGCGACC TCACCATGGA GCAAAAGCTC
  361 ATTTCTGAAG AGGACTTGAA TTCCCGCAAG CGCCGGCGGC AGCATGGCCA GCTCTGGTTC
  421 CCTGAGGGTT TCAAAGTGTC AGAGGCCAGC AAGAAGAAGC GGAGAGAGCC CCTCGGCGAG
  481 GACTCAGTCG GCCTCAAGCC CCTGAAGAAT GCCTCAGATG GTGCTCTGAT GGACGACAAT
  541 CAGAACGAGT GGGGAGACGA AGACCTGGAG ACCAAGAAGT TCCGGTTTGA GGAGCCAGTA
  601 GTTCTCCCTG ACCTGAGTGA TCAGACTGAC CACAGGCAGT GGACCCAGCA GCACCTGGAC
  661 GCTGCTGACC TGCGCATGTC TGCCATGGCC CCAACACCGC CTCAGGGGGA GGTGGATGCT
  721 GACTGCATGG ATGTCAATGT TCGAGGACCA GATGGCTTCA CACCCCTCAT GATTGCCTCC
  781 TGCAGTGNAN GGGGCCTTGA GACANGCAAC AGTGAANAAN AANAAGANGC ACCTGCTGNC
  841 ATCTCTGANN NCNTCTACNN NGGCGNCAGC TTGCACAACC AGANAGACCG CNNCNGGGGA
  901 GANCGNCTNN CACTTGNCTG CCNNANACTC TCNTTCANAN GCTNNNAANC GCTNGNTNGN
961 NNGNNANNGN NNNNNNNNN
//
Primer: RDB06771#1 (Pr0422)
Region: insert mid(insert -Myc-insert )
Sequence file: RDB18622_B0Jlb.seq check
>06771_18622_B0Jl_1_RDB06771#1_H01_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNNGGNG TGCNNAGGCA GANTGCTGGG GCCCAGCGGT TGTACATCTG
   61 CCTGACTGGG CTCCCCACTC AAGAAGCTCC GGCCCAGGTG CCCATTGGCT GCCGAGCTCA
  121 CACTGAGGTG TGGCTGTGAT GGTGGCTGCA GGTTCTGAGG CTGGAGCTGT AAGTTCTGTG
  181 GCTGCACCTG CTGGGTCTGC ACCAGGTGAG GCTGTGTTGC CAGCCGTGTG TTGGGCAGGC
  241 CCTGGTAAAT AATCGGGGAC AAGGTATTGG TGGAGAGGCT GCTGTGTAGT GGCCCCATCA
  301 TGCTATGCTG GAGGCCAGCT GCCTGTGTGC TCAGTGTGCC CGGCGTCACA CCCGGCCGTA
  361 GTGGGTTGTA CTGGCTGGGC ACCATGCCAT TCTGGAGCCG GGGAAGCCAC TCACACTGGC
  421 CATTCAAGCT TAGGTCCTCT TCAGAAATGA GCTTTTGCTC GGAGCTTGCC GGTGCACCCA
  481 CGGTGAAATT CATAGCCCCC GTGCCATTGG TACTCAGCAC TGTGCTGGCA CTGGAGGCTA
  541 CAGGCAGGTG GGAGAGGCGT GGCGGGGGTG GCTCANAGGC CAACCGGCTA CCTCCAGCCA
  601 GTGCTGCCAT CTCANGCTTG GCTGCCACAT TNNAGTGGCT GANGCCCAGG TGANTGTCNN
  661 NCATACCANG CNNNGGCTGA NANGNATNNN NGGANACTGC TNNNNNGGGG ANGGNNNNNN
721 NGGGNNNNNN NNNN
//
Primer: hGCSF_R (Pr0031)
Region: hGCSF-3'UTR+pA,insert 3'
Sequence file: RDB18622_B0Jlc.seq check
>06771_18622_B0Jl_1_hGCSF_R_H01_08_ABI08.ab1
    1 NNNNNNNNNN NCNNNNNGTC CNNGGNGGGG NNCCTCACTC TACTAGAACT AGTGGATCCC
   61 CAATTCATGT CTTACGGTCA AGGTTTTTTT TTTTCGATTT TGGAAAGAAG CAATCAATAA
  121 ATAAACAGCA TTTACAAATC ATGAATCCTT GTTCATATTT TACAGACACA CAGGGAACTT
  181 CACCCTGACC AGGAAAATCA AGGCTCTGAG AACTAGTTTA TTTTCTTGGA ACAGATATGT
  241 TTTTCCCTAG TGCCATAGAA AACAATACAT ATAAATAAAA AGGCAGTGTT TCTGTGGAAA
  301 ATAAAAGTAC ATAAATAAAT ACTAAAAAAA AAAATGGAAA TTCTTGTCCT CTTATTTTGT
  361 ATAAAAACAT TTTTTAAAAG GCTCCTTTGG TCGCCCCAGC ATCCTGAAGC ACTGGAAAGG
  421 ACTCCCAACA GGGCTTGGGA ACGGAAGCTG GGGTCCTGCA TCCCACATCT CTGTTTATTT
  481 AAATGCCTCT GGAATGTGGG TGATCTGGGA CGGCATGGTG GTGGGCGGGC TGGAGATGCC
  541 CTCGGACCAA TCAGAGATGT TGGAATGCGG GGAGGAGCTG GACCACTGGT CAGGGGACTC
  601 AGGGGANGGG GTGAGGAAGG NGTGCTCTGG CACCTGCAGC TGGTGGCTGG GGGTGTTGTC
  661 CACAGGGGAN GNNNAGTNNC TGNGCTGGNN ANGANGGGTC NNNNNCTGGN NANNNNNNNT
721 GGNNNGNNNN NNNNNN
//
Primer: Neo-M_R (Pr0145)
Region: NeoR, TK pro
Sequence file: RDB18622_B0Jld.seq check
>06771_18622_B0Jl_1_Neo-M_R_H09_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTTC NNNNCTTNNG TGNCNACNNT CGAGCACAGC
   61 TGCGCAAGGA ACGCCCGTCG TGGCCNGCCA CGATAGCCGC GCTGCCTCGT CCTGCAGTTC
  121 ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG
  181 CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG
  241 CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TGGCCGATCC
  301 CATATTGGCT GCAGGGTCGC TCGGTGTTCG AGGCCACACG CGTCACCTTA ATATGCGAAG
  361 TGGACCTGGG ACCGCGCCGC CCCGACTGCA TCTGCGTGTT CGAATTCGCC AATGACAAGA
  421 CGCTGGGCGG GGTTTGCTCG ACATTGGGTG GAAACATTCC AGGCCTGGGT GGAGAGGCTT
  481 TTTGCTTCCT CTTGCAAAAC CACACTGCTC GACATTGGGT GGAAACATTC CAGGCCTGGG
  541 TGGAGAGGCT TTTTGCTTCC TCTTGAAAAC CACACTGCTC GACTGGCGAA AGGGGGATGT
  601 GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG
661 ACGGCCAGTG AATTCGNNNT TTNNTTNNNN NNNNNTNNNN NNNTTNNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kato, H., Functional conservation of mouse Notch receptor family members. FEBS Lett., 395, 221-224 (1996). PMID 8898100. [link to RRC of NBRP]
user_report Yoneyama, T., Notch Inhibitors from Calotropis gigantea That Induce Neuronal Differentiation of Neural Stem Cells. J. Nat. Prod. 80 (9): 2453-2461 (2017). PMID 28817274. [link to RRC of NBRP]
user_report Kitajima, K., Wnt signaling regulates left-right axis formation in the node of mouse embryos. Dev. Biol., 380 (2): 222-232 (2013). PMID 23707899. [link to RRC of NBRP]
user_report Saito, Y., Casitas B-cell lymphoma mutation in childhood T-cell acute lymphoblastic leukemia. Leuk Res., 36 (8): 1009-1015 (2012). PMID 22591685. [link to RRC of NBRP]
user_report O'Brien, L.L., Wt1a, Foxc1a, and the Notch mediator Rbpj physically interact and regulate the formation of podocytes in zebrafish. Dev. Biol., 358 (2): 318-330 (2011). PMID 21871448. [link to RRC of NBRP]