RDB06771 - pEF-BOSneo-mNotch1 RAMIC

Catalog number	RDB06771
Resource name	pEF-BOSneo-mNotch1 RAMIC
Alternative name	#12249
Clone info.	Expression vector of mouse Notch1 intracellular region. RAMIC is tagged with 6x Myc epitope sequence. Insertion of 12 amino acids sequence of myc-tag (SEQKLISEEDlS) was identified between 2,293 and 2,294aa corresponding Fig.1 of Kato, H., FEBS Lett. 395: 221-224, 1996 (Feb.17, 2017).
Vector backbone	pEF-BOS neo (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Mouse Notch gene homolog 1 cDNA
Depositor	Honjo, Tasuku \|

Other clones in our bank	mouse Notch1 (NCBI Gene 18128) \|

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by DEPOSITOR (FEBS Lett., 395, 221-224, 1996) and an acknowledgment to Depositor are requested. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.
Additional terms and conditions for distribution	Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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Distribution information (domestic users)

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提供条件	本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。営利機関の利用者は、事前に京都大学・「医学領域」産学連携推進機構・知財室 (contracts@kumbl.med.kyoto-u.ac.jp) に連絡し、承諾を得ること。利用者は、研究成果の公表にあたって寄託者の指定する文献(FEBS Lett., 395, 221-224, 1996)を引用し、寄託者への謝辞の表明を必要とする。理研BRCは、本件リソースのリソース名、提供先、利用目的、件数を京都大学・本庶佑教授に報告する
付加的提供条件	pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて」をご覧ください。提供依頼書 [Word] 提供同意書 [Word]

References and tips

Sequence information

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB06771	pEF-BOSneo-mNotch1 RAMIC	DNA solution

Featured content	Dnaconda's recommendation EXR0030j (Japanese text)

original	Kato, H., Functional conservation of mouse Notch receptor family members. FEBS Lett., 395, 221-224 (1996). PMID 8898100.

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Primer: pAxEF_F1	RDB06771_A3Fqa.seq
>06771_06771_A3Fq_pAxEF_F1_A06_03.ab1 1 GGCCTTCTTT CTCATTTCAG TGTCGTGAGG AATTCTCTGA CGGTATCGAT TTAAAGCTAT 61 GGAGCAAAAG CTCATTTCTG AAGAGGACTT GAATGAAATG GAGCAAAAGC TCATTTCTGA 121 AGAGGACTTG AATGAAATGG AGCAAAAGCT CATTTCTGAA GAGGACTTGA ATGAAATGGA 181 GCAAAAGCTC ATTTCTGAAG AGGACTTGAA TGAAATGGAG CAAAAGCTCA TTTCTGAAGA 241 GGACTTGAAT GAAATGGAGA GCTTGGGCGA CCTCACCATG GAGCAAAAGC TCATTTCTGA 301 AGAGGACTTG AATTCCCGCA AGCGCCGGCG GCAGCATGGC CAGCTCTGGT TCCCTGAGGG 361 TTTCAAAGTG TCAGAGGCCA GCAAGAAGAA GCGGAGAGAG CCCCTCGGCG AGGACTCAGT 421 CGGCCTCAAG CCCCTGAAGA ATGCCTCAGA TGGTGCTCTG ATGGACGACA ATCAGAACGA 481 GTGGGGAGAC GAAGACCTGG AGACCAAGAA GTTCCGGTTT GAGGAGCCAG TAGTTCTCCC 541 TGACCTGAGT GATCAGACTG ACCACAGGCA GTGGACCCAG CAGCACCTGG ACGCTGCTGA 601 CCTGCGCATG TCTGCCATGG CCCCAACACC GCCTCAGGGG GAGGTGGATG CTGACTGCAT 661 GGATGTCAAT GTTCGAGGAC CAGATGGCTT CACACCCCTC ATGATTGCCT CCTGCAGTGG 721 AGGGGGCCTT GAGACAGGCA ACAGTGAAGA AGAAGAAGAT GCACCTGCTG TCATCTCTGA 781 CTTCATCTAC CAGGGCGCCA GCTTGCACAA CCAGACAGAC CGCACCGGGG AGACCGCCTT 841 GCACTTGGCT GCCCGATACT CTCGTTCAGA TGCTGCAAAG CGCTTGCTGG AGGCCAGTGC 901 AGATGCCAAC ATCCAGGACA ACATGGGCCG TACTCCGTTA CATGCAGCAG TTTCTGCAGA 961 TGCTCAGGGT GTCTTCCAGA TCCTGCTCCG GGAACAGGGC CACAGATCTG GATGCCCGAA 1021 TTGCATGATG GGCACAACTC CACTGATCCT GGCTGCGCGC TGGCCGTGGA GGGCATGCTG 1081 GAGACCTCAT CACTCACATG CTGACGTCAA TGCGTGTATG AACTTAGCAA GTTCGGCTTT 1141 TGCCAATTG //
Primer: hGCSF-R	RDB06771_A3Fqb.seq
>06771_06771_A3Fq_hGCSF-R_B06_06.ab1 1 GGGAGGGGGA ATGATCTCTC AGGGTCCAGG TGGGGACCCT CACTCTACTA GAACTAGTGG 61 ATCCCCAATT CATGTCTTAC GGTCAAGGTT TTTTTTTTTC GATTTTGGAA AGAAGCAATC 121 AATAAATAAA CAGCATTTAC AAATCATGAA TCCTTGTTCA TATTTTACAG ACACACAGGG 181 AACTTCACCC TGACCAGGAA AATCAAGGCT CTGAGAACTA GTTTATTTTC TTGGAACAGA 241 TATGTTTTTC CCTAGTGCCA TAGAAAACAA TACATATAAA TAAAAAGGCA GTGTTTCTGT 301 GGAAAATAAA AGTACATAAA TAAATACTAA AAAAAAAAAT GGAAATTCTT GTCCTCTTAT 361 TTTGTATAAA AACATTTTTT AAAAGGCTCC TTTGGTCGCC CCAGCATCCT GAAGCACTGG 421 AAAGGACTCC CAACAGGGCT TGGGAACGGA AGCTGGGGTC CTGCATCCCA CATCTCTGTT 481 TATTTAAATG CCTCTGGAAT GTGGGTGATC TGGGACGGCA TGGTGGTGGG CGGGCTGGAG 541 ATGCCCTCGG ACCAATCAGA GATGTTGGAA TGCGGGGAGG AGCTGGACCA CTGGTCAGGG 601 GACTCAGGGG ATGGGGTGAG GAAGGGGTGC TCTGGCACCT GCAGCTGGTG GCTGGGGGTG 661 TTGTCCACAG GGGAGGAGGA GTAACTGTGC TGGGAAGGAG GGGTCAGGAA CTGGGTAGTG 721 GTCATGGGTG GGACCATGGA GGATGGCAGT GATGTGGGCA GGGCCTGGCT TTCCTGGGGC 781 AGAATGGTGT GCACAGGCAG ACTGCTGGGG CCCAGCGGTT GTACATCTGC CTGACTGGGC 841 TCCCCACTCA AGAAGCTCCG GCCCCAGGTG CCCATTGGCT GCCGAGCTCA CACTGAGGTG 901 TGGCTGTGAT GGTGGCTGCA GGTTCTGAAG GCTGGAGCTG TAGTTCTGTG GCTGCACCTG 961 CTGGGGTCCT GCACCAGTGA GGCTGTGTTG CAACCGTGTG TTGGCAGGCC CTGGTAATTA 1021 TCGGGGACAA AGGTATTGGT GGA //
Primer: Neo-M_R	RDB06771_A3Fqc.seq
>06771_06771_A3Fq_Neo-M_R_C06_09.ab1 1 GAGGGGCCAA TGGCAGCCAG TCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA 61 AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCCTGCA GTTCATTCAG 121 GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA 181 CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC 241 CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATGGCCG ATCCCATATT 301 GGCTGCAGGG TCGCTCGGTG TTCGAGGCCA CACGCGTCAC CTTAATATGC GAAGTGGACC 361 TGGGACCGCG CCGCCCCGAC TGCATCTGCG TGTTCGAATT CGCCAATGAC AAGACGCTGG 421 GCGGGGTTTG CTCGACATTG GGTGGAAACA TTCCAGGCCT GGGTGGAGAG GCTTTTTGCT 481 TCCTCTTGCA AAACCACACT GCTCGACATT GGGTGGAAAC ATTCCAGGCC TGGGTGGAGA 541 GGCTTTTTGC TTCCTCTTGA AAACCACACT GCTCGACTGG CGAAAGGGGG ATGTGCTGCA 601 AGGCGATTAA GTTGGGTAAC GCCAGGGTTT TCCCAGTCAC GACGTTGTAA AACGACGGCC 661 AGTGAATTCG GTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT 721 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTGGGAGG GAAACACTTT TTTATCCCGA 781 GATTCTCTTT CAATGGGACA GGGGGGGGGA CACTGGGGAG GGGGGGGGGA AAAAGGGGAG 841 GG //

original	Kato, H., Functional conservation of mouse Notch receptor family members. FEBS Lett., 395, 221-224 (1996). PMID 8898100.
user report	Yoneyama, T., Notch Inhibitors from Calotropis gigantea That Induce Neuronal Differentiation of Neural Stem Cells. J. Nat. Prod. 80 (9): 2453-2461 (2017). PMID 28817274.
user report	Kitajima, K., Wnt signaling regulates left-right axis formation in the node of mouse embryos. Dev. Biol., 380 (2): 222-232 (2013). PMID 23707899.
user report	Saito, Y., Casitas B-cell lymphoma mutation in childhood T-cell acute lymphoblastic leukemia. Leuk Res., 36 (8): 1009-1015 (2012). PMID 22591685.
user report	O'Brien, L.L., Wt1a, Foxc1a, and the Notch mediator Rbpj physically interact and regulate the formation of podocytes in zebrafish. Dev. Biol., 358 (2): 318-330 (2011). PMID 21871448.