Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEF-BOSneo-mNotch1 RAMIC

Expression vector of mouse Notch1 intracellular region

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by DEPOSITOR (FEBS Lett., 395, 221-224, 1996) and an acknowledgment to Depositor are requested. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.
Additional terms and conditions for distribution	Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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Distribution information (domestic users) [open/close]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB06771_A3Fqp1_2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxEF_F1 Sequence file: RDB06771_A3Fqa.seq
>06771_06771_A3Fq_pAxEF_F1_A06_03.ab1     1 GGCCTTCTTT CTCATTTCAG TGTCGTGAGG AATTCTCTGA CGGTATCGAT TTAAAGCTAT    61 GGAGCAAAAG CTCATTTCTG AAGAGGACTT GAATGAAATG GAGCAAAAGC TCATTTCTGA   121 AGAGGACTTG AATGAAATGG AGCAAAAGCT CATTTCTGAA GAGGACTTGA ATGAAATGGA   181 GCAAAAGCTC ATTTCTGAAG AGGACTTGAA TGAAATGGAG CAAAAGCTCA TTTCTGAAGA   241 GGACTTGAAT GAAATGGAGA GCTTGGGCGA CCTCACCATG GAGCAAAAGC TCATTTCTGA   301 AGAGGACTTG AATTCCCGCA AGCGCCGGCG GCAGCATGGC CAGCTCTGGT TCCCTGAGGG   361 TTTCAAAGTG TCAGAGGCCA GCAAGAAGAA GCGGAGAGAG CCCCTCGGCG AGGACTCAGT   421 CGGCCTCAAG CCCCTGAAGA ATGCCTCAGA TGGTGCTCTG ATGGACGACA ATCAGAACGA   481 GTGGGGAGAC GAAGACCTGG AGACCAAGAA GTTCCGGTTT GAGGAGCCAG TAGTTCTCCC   541 TGACCTGAGT GATCAGACTG ACCACAGGCA GTGGACCCAG CAGCACCTGG ACGCTGCTGA   601 CCTGCGCATG TCTGCCATGG CCCCAACACC GCCTCAGGGG GAGGTGGATG CTGACTGCAT   661 GGATGTCAAT GTTCGAGGAC CAGATGGCTT CACACCCCTC ATGATTGCCT CCTGCAGTGG   721 AGGGGGCCTT GAGACAGGCA ACAGTGAAGA AGAAGAAGAT GCACCTGCTG TCATCTCTGA   781 CTTCATCTAC CAGGGCGCCA GCTTGCACAA CCAGACAGAC CGCACCGGGG AGACCGCCTT   841 GCACTTGGCT GCCCGATACT CTCGTTCAGA TGCTGCAAAG CGCTTGCTGG AGGCCAGTGC   901 AGATGCCAAC ATCCAGGACA ACATGGGCCG TACTCCGTTA CATGCAGCAG TTTCTGCAGA   961 TGCTCAGGGT GTCTTCCAGA TCCTGCTCCG GGAACAGGGC CACAGATCTG GATGCCCGAA  1021 TTGCATGATG GGCACAACTC CACTGATCCT GGCTGCGCGC TGGCCGTGGA GGGCATGCTG  1081 GAGACCTCAT CACTCACATG CTGACGTCAA TGCGTGTATG AACTTAGCAA GTTCGGCTTT 1141 TGCCAATTG //
Primer: hGCSF-R Sequence file: RDB06771_A3Fqb.seq
>06771_06771_A3Fq_hGCSF-R_B06_06.ab1     1 GGGAGGGGGA ATGATCTCTC AGGGTCCAGG TGGGGACCCT CACTCTACTA GAACTAGTGG    61 ATCCCCAATT CATGTCTTAC GGTCAAGGTT TTTTTTTTTC GATTTTGGAA AGAAGCAATC   121 AATAAATAAA CAGCATTTAC AAATCATGAA TCCTTGTTCA TATTTTACAG ACACACAGGG   181 AACTTCACCC TGACCAGGAA AATCAAGGCT CTGAGAACTA GTTTATTTTC TTGGAACAGA   241 TATGTTTTTC CCTAGTGCCA TAGAAAACAA TACATATAAA TAAAAAGGCA GTGTTTCTGT   301 GGAAAATAAA AGTACATAAA TAAATACTAA AAAAAAAAAT GGAAATTCTT GTCCTCTTAT   361 TTTGTATAAA AACATTTTTT AAAAGGCTCC TTTGGTCGCC CCAGCATCCT GAAGCACTGG   421 AAAGGACTCC CAACAGGGCT TGGGAACGGA AGCTGGGGTC CTGCATCCCA CATCTCTGTT   481 TATTTAAATG CCTCTGGAAT GTGGGTGATC TGGGACGGCA TGGTGGTGGG CGGGCTGGAG   541 ATGCCCTCGG ACCAATCAGA GATGTTGGAA TGCGGGGAGG AGCTGGACCA CTGGTCAGGG   601 GACTCAGGGG ATGGGGTGAG GAAGGGGTGC TCTGGCACCT GCAGCTGGTG GCTGGGGGTG   661 TTGTCCACAG GGGAGGAGGA GTAACTGTGC TGGGAAGGAG GGGTCAGGAA CTGGGTAGTG   721 GTCATGGGTG GGACCATGGA GGATGGCAGT GATGTGGGCA GGGCCTGGCT TTCCTGGGGC   781 AGAATGGTGT GCACAGGCAG ACTGCTGGGG CCCAGCGGTT GTACATCTGC CTGACTGGGC   841 TCCCCACTCA AGAAGCTCCG GCCCCAGGTG CCCATTGGCT GCCGAGCTCA CACTGAGGTG   901 TGGCTGTGAT GGTGGCTGCA GGTTCTGAAG GCTGGAGCTG TAGTTCTGTG GCTGCACCTG   961 CTGGGGTCCT GCACCAGTGA GGCTGTGTTG CAACCGTGTG TTGGCAGGCC CTGGTAATTA 1021 TCGGGGACAA AGGTATTGGT GGA //
Primer: Neo-M_R Sequence file: RDB06771_A3Fqc.seq
>06771_06771_A3Fq_Neo-M_R_C06_09.ab1     1 GAGGGGCCAA TGGCAGCCAG TCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA    61 AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCCTGCA GTTCATTCAG   121 GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA   181 CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC   241 CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATGGCCG ATCCCATATT   301 GGCTGCAGGG TCGCTCGGTG TTCGAGGCCA CACGCGTCAC CTTAATATGC GAAGTGGACC   361 TGGGACCGCG CCGCCCCGAC TGCATCTGCG TGTTCGAATT CGCCAATGAC AAGACGCTGG   421 GCGGGGTTTG CTCGACATTG GGTGGAAACA TTCCAGGCCT GGGTGGAGAG GCTTTTTGCT   481 TCCTCTTGCA AAACCACACT GCTCGACATT GGGTGGAAAC ATTCCAGGCC TGGGTGGAGA   541 GGCTTTTTGC TTCCTCTTGA AAACCACACT GCTCGACTGG CGAAAGGGGG ATGTGCTGCA   601 AGGCGATTAA GTTGGGTAAC GCCAGGGTTT TCCCAGTCAC GACGTTGTAA AACGACGGCC   661 AGTGAATTCG GTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT   721 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTGGGAGG GAAACACTTT TTTATCCCGA   781 GATTCTCTTT CAATGGGACA GGGGGGGGGA CACTGGGGAG GGGGGGGGGA AAAAGGGGAG 841 GG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2020.01.22

GNP_filter3_RDBDEP_html_191130.pl