Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-BOSneo-mNotch1 RAMIC

Expression vector of mouse Notch1 intracellular region

Catalog number RDB06771
Resource name pEF-BOSneo-mNotch1 RAMIC
Alternative name #12249
Clone info. Expression vector of mouse Notch1 intracellular region. RAMIC is tagged with 6x Myc epitope sequence.
Comment Insertion of 12 amino acids sequence of myc-tag (SEQKLISEEDlS) was identified between 2,293 and 2,294aa corresponding Fig.1 of Kato, H., FEBS Lett. 395: 221-224, 1996 (Feb.17, 2017).
Vector backbone pEF-BOS neo (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Notch gene homolog 1 cDNA
Depositor|Developer Honjo, Tasuku |
Other clones in our bank

External Database
Mouse Notch gene homolog 1

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by DEPOSITOR (FEBS Lett., 395, 221-224, 1996) and an acknowledgment to Depositor are requested. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.
          Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。営利機関の利用者は、事前に京都大学・「医学領域」産学連携推進機構・知財室 (contracts@kumbl.med.kyoto-u.ac.jp) に連絡し、承諾を得ること。利用者は、研究成果の公表にあたって寄託者の指定する文献(FEBS Lett., 395, 221-224, 1996)を引用し、寄託者への謝辞の表明を必要とする。理研BRCは、本件リソースのリソース名、提供先、利用目的、件数を京都大学・本庶佑教授に報告する
          付加的提供条件 pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB06771 pEF-BOSneo-mNotch1 RAMIC DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Kato, H., Functional conservation of mouse Notch receptor family members. FEBS Lett., 395, 221-224 (1996). PMID 8898100.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Dnaconda's recommendation EXR0030j (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18622_B0Jlp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EF1a-C_F
          Region: EF-1 alpha pro,Myc x6,insert 5'
          Sequence file: RDB18622_B0Jla.seq check
          >06771_18622_B0Jl_1_EF1a-C_F_F09_06_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNTTT NNANTTTGGN NCTTGGTTCN TTCTCNAGCC TCAGACAGTG
             61 GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGAA TTCTCTGACG GTATCGATTT
            121 AAAGCTATGG AGCAAAAGCT CATTTCTGAA GAGGACTTGA ATGAAATGGA GCAAAAGCTC
            181 ATTTCTGAAG AGGACTTGAA TGAAATGGAG CAAAAGCTCA TTTCTGAAGA GGACTTGAAT
            241 GAAATGGAGC AAAAGCTCAT TTCTGAAGAG GACTTGAATG AAATGGAGCA AAAGCTCATT
            301 TCTGAAGAGG ACTTGAATGA AATGGAGAGC TTGGGCGACC TCACCATGGA GCAAAAGCTC
            361 ATTTCTGAAG AGGACTTGAA TTCCCGCAAG CGCCGGCGGC AGCATGGCCA GCTCTGGTTC
            421 CCTGAGGGTT TCAAAGTGTC AGAGGCCAGC AAGAAGAAGC GGAGAGAGCC CCTCGGCGAG
            481 GACTCAGTCG GCCTCAAGCC CCTGAAGAAT GCCTCAGATG GTGCTCTGAT GGACGACAAT
            541 CAGAACGAGT GGGGAGACGA AGACCTGGAG ACCAAGAAGT TCCGGTTTGA GGAGCCAGTA
            601 GTTCTCCCTG ACCTGAGTGA TCAGACTGAC CACAGGCAGT GGACCCAGCA GCACCTGGAC
            661 GCTGCTGACC TGCGCATGTC TGCCATGGCC CCAACACCGC CTCAGGGGGA GGTGGATGCT
            721 GACTGCATGG ATGTCAATGT TCGAGGACCA GATGGCTTCA CACCCCTCAT GATTGCCTCC
            781 TGCAGTGNAN GGGGCCTTGA GACANGCAAC AGTGAANAAN AANAAGANGC ACCTGCTGNC
            841 ATCTCTGANN NCNTCTACNN NGGCGNCAGC TTGCACAACC AGANAGACCG CNNCNGGGGA
            901 GANCGNCTNN CACTTGNCTG CCNNANACTC TCNTTCANAN GCTNNNAANC GCTNGNTNGN
          961 NNGNNANNGN NNNNNNNNN
          //
          Primer: RDB06771#1
          Region: insert mid(insert -Myc-insert )
          Sequence file: RDB18622_B0Jlb.seq check
          >06771_18622_B0Jl_1_RDB06771#1_H01_08_ABI08.ab1
              1 NNNNNNNNNN NNNNNNGGNG TGCNNAGGCA GANTGCTGGG GCCCAGCGGT TGTACATCTG
             61 CCTGACTGGG CTCCCCACTC AAGAAGCTCC GGCCCAGGTG CCCATTGGCT GCCGAGCTCA
            121 CACTGAGGTG TGGCTGTGAT GGTGGCTGCA GGTTCTGAGG CTGGAGCTGT AAGTTCTGTG
            181 GCTGCACCTG CTGGGTCTGC ACCAGGTGAG GCTGTGTTGC CAGCCGTGTG TTGGGCAGGC
            241 CCTGGTAAAT AATCGGGGAC AAGGTATTGG TGGAGAGGCT GCTGTGTAGT GGCCCCATCA
            301 TGCTATGCTG GAGGCCAGCT GCCTGTGTGC TCAGTGTGCC CGGCGTCACA CCCGGCCGTA
            361 GTGGGTTGTA CTGGCTGGGC ACCATGCCAT TCTGGAGCCG GGGAAGCCAC TCACACTGGC
            421 CATTCAAGCT TAGGTCCTCT TCAGAAATGA GCTTTTGCTC GGAGCTTGCC GGTGCACCCA
            481 CGGTGAAATT CATAGCCCCC GTGCCATTGG TACTCAGCAC TGTGCTGGCA CTGGAGGCTA
            541 CAGGCAGGTG GGAGAGGCGT GGCGGGGGTG GCTCANAGGC CAACCGGCTA CCTCCAGCCA
            601 GTGCTGCCAT CTCANGCTTG GCTGCCACAT TNNAGTGGCT GANGCCCAGG TGANTGTCNN
            661 NCATACCANG CNNNGGCTGA NANGNATNNN NGGANACTGC TNNNNNGGGG ANGGNNNNNN
          721 NGGGNNNNNN NNNN
          //
          Primer: hGCSF_R
          Region: hGCSF-3'UTR+pA,insert 3'
          Sequence file: RDB18622_B0Jlc.seq check
          >06771_18622_B0Jl_1_hGCSF_R_H01_08_ABI08.ab1
              1 NNNNNNNNNN NCNNNNNGTC CNNGGNGGGG NNCCTCACTC TACTAGAACT AGTGGATCCC
             61 CAATTCATGT CTTACGGTCA AGGTTTTTTT TTTTCGATTT TGGAAAGAAG CAATCAATAA
            121 ATAAACAGCA TTTACAAATC ATGAATCCTT GTTCATATTT TACAGACACA CAGGGAACTT
            181 CACCCTGACC AGGAAAATCA AGGCTCTGAG AACTAGTTTA TTTTCTTGGA ACAGATATGT
            241 TTTTCCCTAG TGCCATAGAA AACAATACAT ATAAATAAAA AGGCAGTGTT TCTGTGGAAA
            301 ATAAAAGTAC ATAAATAAAT ACTAAAAAAA AAAATGGAAA TTCTTGTCCT CTTATTTTGT
            361 ATAAAAACAT TTTTTAAAAG GCTCCTTTGG TCGCCCCAGC ATCCTGAAGC ACTGGAAAGG
            421 ACTCCCAACA GGGCTTGGGA ACGGAAGCTG GGGTCCTGCA TCCCACATCT CTGTTTATTT
            481 AAATGCCTCT GGAATGTGGG TGATCTGGGA CGGCATGGTG GTGGGCGGGC TGGAGATGCC
            541 CTCGGACCAA TCAGAGATGT TGGAATGCGG GGAGGAGCTG GACCACTGGT CAGGGGACTC
            601 AGGGGANGGG GTGAGGAAGG NGTGCTCTGG CACCTGCAGC TGGTGGCTGG GGGTGTTGTC
            661 CACAGGGGAN GNNNAGTNNC TGNGCTGGNN ANGANGGGTC NNNNNCTGGN NANNNNNNNT
          721 GGNNNGNNNN NNNNNN
          //
          Primer: Neo-M_R
          Region: NeoR, TK pro
          Sequence file: RDB18622_B0Jld.seq check
          >06771_18622_B0Jl_1_Neo-M_R_H09_08_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTTC NNNNCTTNNG TGNCNACNNT CGAGCACAGC
             61 TGCGCAAGGA ACGCCCGTCG TGGCCNGCCA CGATAGCCGC GCTGCCTCGT CCTGCAGTTC
            121 ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG
            181 CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG
            241 CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TGGCCGATCC
            301 CATATTGGCT GCAGGGTCGC TCGGTGTTCG AGGCCACACG CGTCACCTTA ATATGCGAAG
            361 TGGACCTGGG ACCGCGCCGC CCCGACTGCA TCTGCGTGTT CGAATTCGCC AATGACAAGA
            421 CGCTGGGCGG GGTTTGCTCG ACATTGGGTG GAAACATTCC AGGCCTGGGT GGAGAGGCTT
            481 TTTGCTTCCT CTTGCAAAAC CACACTGCTC GACATTGGGT GGAAACATTC CAGGCCTGGG
            541 TGGAGAGGCT TTTTGCTTCC TCTTGAAAAC CACACTGCTC GACTGGCGAA AGGGGGATGT
            601 GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG
          661 ACGGCCAGTG AATTCGNNNT TTNNTTNNNN NNNNNTNNNN NNNTTNNNNN NNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kato, H., Functional conservation of mouse Notch receptor family members. FEBS Lett., 395, 221-224 (1996). PMID 8898100.
          user_report Yoneyama, T., Notch Inhibitors from Calotropis gigantea That Induce Neuronal Differentiation of Neural Stem Cells. J. Nat. Prod. 80 (9): 2453-2461 (2017). PMID 28817274.
          user_report Kitajima, K., Wnt signaling regulates left-right axis formation in the node of mouse embryos. Dev. Biol., 380 (2): 222-232 (2013). PMID 23707899.
          user_report Saito, Y., Casitas B-cell lymphoma mutation in childhood T-cell acute lymphoblastic leukemia. Leuk Res., 36 (8): 1009-1015 (2012). PMID 22591685.
          user_report O'Brien, L.L., Wt1a, Foxc1a, and the Notch mediator Rbpj physically interact and regulate the formation of podocytes in zebrafish. Dev. Biol., 358 (2): 318-330 (2011). PMID 21871448.

          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl