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pEF-BOSneo-mNotch1 RAMIC

Expression vector of mouse Notch1 intracellular region

Catalog number RDB06771
Resource name pEF-BOSneo-mNotch1 RAMIC
Alternative name #12249
Clone info. Expression vector of mouse Notch1 intracellular region. RAMIC is tagged with 6x Myc epitope sequence.
Insertion of 12 amino acids sequence of myc-tag (SEQKLISEEDlS) was identified between 2,293 and 2,294aa corresponding Fig.1 of Kato, H., FEBS Lett. 395: 221-224, 1996 (Feb.17, 2017).
Vector backbone pEF-BOS neo (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Notch gene homolog 1 cDNA
Depositor Honjo, Tasuku |
 
Other clones in our bank mouse Notch1 (NCBI Gene 18128) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by DEPOSITOR (FEBS Lett., 395, 221-224, 1996) and an acknowledgment to Depositor are requested. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.
Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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RDB06771 pEF-BOSneo-mNotch1 RAMIC DNA solution

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References and tips

Electronic file

Featured content

Featured content Dnaconda's recommendation EXR0030j (Japanese text)

References

original Kato, H., Functional conservation of mouse Notch receptor family members. FEBS Lett., 395, 221-224 (1996). PMID 8898100.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB06771_A3Fqp1_2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxEF_F1 RDB06771_A3Fqa.seq
>06771_06771_A3Fq_pAxEF_F1_A06_03.ab1
    1 GGCCTTCTTT CTCATTTCAG TGTCGTGAGG AATTCTCTGA CGGTATCGAT TTAAAGCTAT
   61 GGAGCAAAAG CTCATTTCTG AAGAGGACTT GAATGAAATG GAGCAAAAGC TCATTTCTGA
  121 AGAGGACTTG AATGAAATGG AGCAAAAGCT CATTTCTGAA GAGGACTTGA ATGAAATGGA
  181 GCAAAAGCTC ATTTCTGAAG AGGACTTGAA TGAAATGGAG CAAAAGCTCA TTTCTGAAGA
  241 GGACTTGAAT GAAATGGAGA GCTTGGGCGA CCTCACCATG GAGCAAAAGC TCATTTCTGA
  301 AGAGGACTTG AATTCCCGCA AGCGCCGGCG GCAGCATGGC CAGCTCTGGT TCCCTGAGGG
  361 TTTCAAAGTG TCAGAGGCCA GCAAGAAGAA GCGGAGAGAG CCCCTCGGCG AGGACTCAGT
  421 CGGCCTCAAG CCCCTGAAGA ATGCCTCAGA TGGTGCTCTG ATGGACGACA ATCAGAACGA
  481 GTGGGGAGAC GAAGACCTGG AGACCAAGAA GTTCCGGTTT GAGGAGCCAG TAGTTCTCCC
  541 TGACCTGAGT GATCAGACTG ACCACAGGCA GTGGACCCAG CAGCACCTGG ACGCTGCTGA
  601 CCTGCGCATG TCTGCCATGG CCCCAACACC GCCTCAGGGG GAGGTGGATG CTGACTGCAT
  661 GGATGTCAAT GTTCGAGGAC CAGATGGCTT CACACCCCTC ATGATTGCCT CCTGCAGTGG
  721 AGGGGGCCTT GAGACAGGCA ACAGTGAAGA AGAAGAAGAT GCACCTGCTG TCATCTCTGA
  781 CTTCATCTAC CAGGGCGCCA GCTTGCACAA CCAGACAGAC CGCACCGGGG AGACCGCCTT
  841 GCACTTGGCT GCCCGATACT CTCGTTCAGA TGCTGCAAAG CGCTTGCTGG AGGCCAGTGC
  901 AGATGCCAAC ATCCAGGACA ACATGGGCCG TACTCCGTTA CATGCAGCAG TTTCTGCAGA
  961 TGCTCAGGGT GTCTTCCAGA TCCTGCTCCG GGAACAGGGC CACAGATCTG GATGCCCGAA
 1021 TTGCATGATG GGCACAACTC CACTGATCCT GGCTGCGCGC TGGCCGTGGA GGGCATGCTG
 1081 GAGACCTCAT CACTCACATG CTGACGTCAA TGCGTGTATG AACTTAGCAA GTTCGGCTTT
1141 TGCCAATTG
//
Primer: hGCSF-R RDB06771_A3Fqb.seq
>06771_06771_A3Fq_hGCSF-R_B06_06.ab1
    1 GGGAGGGGGA ATGATCTCTC AGGGTCCAGG TGGGGACCCT CACTCTACTA GAACTAGTGG
   61 ATCCCCAATT CATGTCTTAC GGTCAAGGTT TTTTTTTTTC GATTTTGGAA AGAAGCAATC
  121 AATAAATAAA CAGCATTTAC AAATCATGAA TCCTTGTTCA TATTTTACAG ACACACAGGG
  181 AACTTCACCC TGACCAGGAA AATCAAGGCT CTGAGAACTA GTTTATTTTC TTGGAACAGA
  241 TATGTTTTTC CCTAGTGCCA TAGAAAACAA TACATATAAA TAAAAAGGCA GTGTTTCTGT
  301 GGAAAATAAA AGTACATAAA TAAATACTAA AAAAAAAAAT GGAAATTCTT GTCCTCTTAT
  361 TTTGTATAAA AACATTTTTT AAAAGGCTCC TTTGGTCGCC CCAGCATCCT GAAGCACTGG
  421 AAAGGACTCC CAACAGGGCT TGGGAACGGA AGCTGGGGTC CTGCATCCCA CATCTCTGTT
  481 TATTTAAATG CCTCTGGAAT GTGGGTGATC TGGGACGGCA TGGTGGTGGG CGGGCTGGAG
  541 ATGCCCTCGG ACCAATCAGA GATGTTGGAA TGCGGGGAGG AGCTGGACCA CTGGTCAGGG
  601 GACTCAGGGG ATGGGGTGAG GAAGGGGTGC TCTGGCACCT GCAGCTGGTG GCTGGGGGTG
  661 TTGTCCACAG GGGAGGAGGA GTAACTGTGC TGGGAAGGAG GGGTCAGGAA CTGGGTAGTG
  721 GTCATGGGTG GGACCATGGA GGATGGCAGT GATGTGGGCA GGGCCTGGCT TTCCTGGGGC
  781 AGAATGGTGT GCACAGGCAG ACTGCTGGGG CCCAGCGGTT GTACATCTGC CTGACTGGGC
  841 TCCCCACTCA AGAAGCTCCG GCCCCAGGTG CCCATTGGCT GCCGAGCTCA CACTGAGGTG
  901 TGGCTGTGAT GGTGGCTGCA GGTTCTGAAG GCTGGAGCTG TAGTTCTGTG GCTGCACCTG
  961 CTGGGGTCCT GCACCAGTGA GGCTGTGTTG CAACCGTGTG TTGGCAGGCC CTGGTAATTA
1021 TCGGGGACAA AGGTATTGGT GGA
//
Primer: Neo-M_R RDB06771_A3Fqc.seq
>06771_06771_A3Fq_Neo-M_R_C06_09.ab1
    1 GAGGGGCCAA TGGCAGCCAG TCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA
   61 AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCCTGCA GTTCATTCAG
  121 GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA
  181 CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC
  241 CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATGGCCG ATCCCATATT
  301 GGCTGCAGGG TCGCTCGGTG TTCGAGGCCA CACGCGTCAC CTTAATATGC GAAGTGGACC
  361 TGGGACCGCG CCGCCCCGAC TGCATCTGCG TGTTCGAATT CGCCAATGAC AAGACGCTGG
  421 GCGGGGTTTG CTCGACATTG GGTGGAAACA TTCCAGGCCT GGGTGGAGAG GCTTTTTGCT
  481 TCCTCTTGCA AAACCACACT GCTCGACATT GGGTGGAAAC ATTCCAGGCC TGGGTGGAGA
  541 GGCTTTTTGC TTCCTCTTGA AAACCACACT GCTCGACTGG CGAAAGGGGG ATGTGCTGCA
  601 AGGCGATTAA GTTGGGTAAC GCCAGGGTTT TCCCAGTCAC GACGTTGTAA AACGACGGCC
  661 AGTGAATTCG GTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT
  721 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTGGGAGG GAAACACTTT TTTATCCCGA
  781 GATTCTCTTT CAATGGGACA GGGGGGGGGA CACTGGGGAG GGGGGGGGGA AAAAGGGGAG
841 GG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Kato, H., Functional conservation of mouse Notch receptor family members. FEBS Lett., 395, 221-224 (1996). PMID 8898100.
user report Yoneyama, T., Notch Inhibitors from Calotropis gigantea That Induce Neuronal Differentiation of Neural Stem Cells. J. Nat. Prod. 80 (9): 2453-2461 (2017). PMID 28817274.
user report Kitajima, K., Wnt signaling regulates left-right axis formation in the node of mouse embryos. Dev. Biol., 380 (2): 222-232 (2013). PMID 23707899.
user report Saito, Y., Casitas B-cell lymphoma mutation in childhood T-cell acute lymphoblastic leukemia. Leuk Res., 36 (8): 1009-1015 (2012). PMID 22591685.
user report O'Brien, L.L., Wt1a, Foxc1a, and the Notch mediator Rbpj physically interact and regulate the formation of podocytes in zebrafish. Dev. Biol., 358 (2): 318-330 (2011). PMID 21871448.

2019.03.08

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