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pCMFlag_hsGATA1

Expression vector of human GATA1.

Catalog number RDB06753
Resource name pCMFlag_hsGATA1
Alternative name SET-0120_1
Clone info. Expression vector of human GATA1. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment PCR cloning, forward primer: gagttccctggcctggggtc; reverse primer: tcatgagctgagcggagccac.
Vector backbone pCMV_S-FLAG (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human GATA1 cDNA
Depositor|Developer DNA Bank, |
 
Sequence (full) RDB06753hts01.seqcheck assembled from experimentally sequenced data
Remarks, protocol and/or map (pdf) RDB06753.pdf
Test sheet RDB06753C0p1-1.pdf 

Distribution information

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提供案内 (日本国内) [open/close]

必要書類
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06753 pCMFlag_hsGATA1 DNA solution

How to cite this biological resource

Materials & Methods section:

The pCMFlag_hsGATA1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06753).

Reference section:

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

user_report Nishino, T., Antagonizing effect of CLPABP on the function of HuR as a regulator of ARE-containing leptin mRNA stability and the effect of its depletion on obesity in old male mouse. Biochim. Biophys. Acta 1861 (11): 1816-1827 (2016). PMID 27616329. [link to RRC of NBRP]