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pCMFlag_hsGATA1

Expression vector of human GATA1.

Catalog number RDB06753
Resource name pCMFlag_hsGATA1
Alternative name SET-0120_1
Clone info. Expression vector of human GATA1. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment PCR cloning, forward primer: gagttccctggcctggggtc; reverse primer: tcatgagctgagcggagccac.
Vector backbone pCMV_S-FLAG (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human GATA1 cDNA
Depositor|Developer DNA Bank, |
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External Database
Human GATA1

          Reference sequence
            

          Distribution information

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          RDB06753 pCMFlag_hsGATA1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB06753.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content pCMV_tag and pRSV_tag assay-ready clones (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13039_A6Cep1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB13039_A6Cea.seq check
          >06753_13039_A6Ce_CMV-Forward_A08_02.ab1
              1 TTTTCACGCC GCGCGGATTG ACGCAGATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
             61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
            121 CGACTCACTA TAGGGAGACC CAAGCTGAAT TCACCATGGA CTACAAGGAC GATGACGATA
            181 AGGCGGCCGA GTTCCCTGGC CTGGGGTCCC TGGGGACCTC AGAGCCCCTC CCCCAGTTTG
            241 TGGATCCTGC TCTGGTGTCC TCCACACCAG AATCAGGGGT TTTCTTCCCC TCTGGGCCTG
            301 AGGGCTTGGA TGCAGCAGCT TCCTCCACTG CCCCGAGCAC AGCCACCGCT GCAGCTGCGG
            361 CACTGGCCTA CTACAGGGAC GCTGAGGCCT ACAGACACTC CCCAGTCTTT CAGGTGTACC
            421 CATTGCTCAA CTGTATGGAG GGGATCCCAG GGGGCTCACC ATATGCCGGC TGGGCCTACG
            481 GCAAGACGGG GCTCTACCCT GCCTCAACTG TGTGTCCCAC CCGCGAGGAC TCTCCTCCCC
            541 AGGCCGTGGA AGATCTGGAT GGAAAAGGCA GCACCAGCTT CCTGGAGACT TTGAAGACAG
            601 AGCGGCTGAG CCCAGACCTC CTGACCCTGG GACCTGCACT GCCTTCATCA CTCCCTGTCC
            661 CCAATAGTGC TTATGGGGGC CCTGACTTTT CCAGTACCTT CTTTTCTCCC ACCGGGAGCC
            721 CCCTCAATTC AGCAGCCTAT TCCTCTCCCA AGCTTCGTGG AACTCTCCCC CTGCCTCCCT
            781 GTGAGGCCAG GGAGTGTGTG AACTGCGGAG CAACAGCCAC TCCACTGTGG CGGAGGGACA
            841 GGACAGGCCA CTACCTATGC AACGCCTGCG GCCTCTATCA CAAGATGAAT GGGCAGAACA
            901 GGCCCCTTCA TCCGGCCCAA GAAGCGCCTG ATTGTCAGTA AACGGGCAAG GTACTTCAGT
            961 GCACCCAACT GCCCAGACGA CCACCCACGA CACTGTGGCG GAGAAAATGC CCAGTGGGGA
           1021 TCCCGTGTGC AAATGCCCTT GCGGCCTCCT ACCTACTAGC CTACACCAAG TGAACTGGCA
          1081 CTGAACATGC GAAGGAATGG TATTCGACTC GAACCGGCTG AAG
          //
          Primer: BGH_rev
          Sequence file: RDB13039_A6Ceb.seq check
          >06753_13039_A6Ce_BGHrev_B08_05.ab1
              1 GACCTGACAT TTAGGTGACC TATAGAATAG GGCCCTCTAG ATGCATGCTC GAGCGGCCTC
             61 ATGAGCTGAG CGGAGCCACC ACAGTAGTGC TGGTGGTGGG GGGCATGGGG CCTGTGGGGA
            121 AGGAGCCCGT GGGTGAGCCC AGTAGGGGTC CAGGGAAAGG CATGAGGTGG CTAACAGGCC
            181 CTGACAGCAC CACAGGGCCC AGGCCTTGGT AGAGATGGGC AGTACCTGGG GGGCCCAGTG
            241 TCAGGCCTGA AGCCACCTCC CCACAATTCC CGCTACCGCT GCCCCCAGCC ACCACCATAA
            301 AGCCACCAGC TGGTCCTTCG GCTGCTCCTG TGCCTCCCAG ACTGGAGCCC CGTTTCTTTT
            361 TCCCTTTTCC AGATGCCTTG CGGTTTCGAG TCTGAATACC ATCCTTCCGC ATGGTCAGTG
            421 GCCGGTTCAC CTGGTGTAGC TTGTAGTAGA GGCCGCAGGC ATTGCACACG GGATCCCCAC
            481 TGGCATTTCT CCGCCACAGT GTCGTGGTGG TCGTCTGGCA GTTGGTGCAC TGAGTACCTG
            541 CCCGTTTACT GACAATCAGG CGCTTCTTGG GCCGGATGAG GGGCCTGTTC TGCCCATTCA
            601 TCTTGTGATA GAGGCCGCAG GCGTTGCATA GGTAGTGGCC TGTCCTGTCC CTCCGCCACA
            661 GTGGAGTGGC TGTTGCTCCG CAGTTCACAC ACTCCCTGGC CTCACAGGGA GGCAGGGGGA
            721 GAGTTCCACG AAGCTTGGGA GAGGAATAGG CTGCTGAATT GAGGGGGCTC CCGGTGGGAG
            781 AAAAGAAGGT ACTGGAAAAG TCAGGGCCCC CATAAGCACT ATTGGGGACA GGGAGTGATG
            841 AAGGCAGTGC AGGTCCCAGG GTCAGGAGGT CTGGGCTCAG CCGCTCTGTC TTCAAAGTCT
            901 CCAGGAAGCT GGTGCTGCCT TTTCCATCCA GATCTTCCAC GGGCCTGGGG AGGGAGAGTC
            961 CTCGCGGGTG GGACACACAG TTTGAGGCAG GGTAGAGCCC CCGTCTTGCC CGTAGGCCCA
           1021 GCCGGCATAT GGTTGAGCCC TCCTGGGATC CCGTCATACA GTTTGGAGCC AATGGTACAT
          1081 CTTGAAGGAA CTGGGGAAGT GTTCTGTGTA AGGGTCT
          //
          Primer: SV40pro_F_V2
          Sequence file: RDB13039_A6Cec.seq check
          >06753_13039_A6Ce_SV40pro_F_V2_C08_08.ab1
              1 CTGGGCGCTT TTTTTTGGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGGATAA
             61 TCCAATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA
            121 TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC
            181 ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC
            241 GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC
            301 GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC
            361 TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC
            421 TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC
            481 GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG
            541 TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT
            601 CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT
            661 CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG
            721 ATTCATCGAC TGTGGCCGGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA
            781 CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AAATGGGCTG ACCGCTTCCT CGTGCTTTAC
            841 GGTATCGCCG CTCCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAAGTTCT
            901 TCTGAAGCGG GACTCTGGGG GTTCGAAATG ACCGACCAAG CCGAACGCCC CAACCCTGCC
            961 CATCACGAAG ATTTCGGAAT TCCCACCGCC CGCCCTTCTA TGGAAAGGTT TGGGGCTTTC
           1021 GGGAAATTCG TTTTTTCCCG GGGAACGGCC GGCTTGGGAA TGAGATCCCC TCCAGCGGCC
           1081 GGGGGAATTC TCAATGGCTT GGGAAGGTTC TTTCGTCGAC CTGAAACCTT TGATTTAATT
          1141 GCCAAGCCTT ATTAAACTTG GGCGTTTA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          user_report Nishino, T., Antagonizing effect of CLPABP on the function of HuR as a regulator of ARE-containing leptin mRNA stability and the effect of its depletion on obesity in old male mouse. Biochim. Biophys. Acta 1861 (11): 1816-1827 (2016). PMID 27616329.

          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl