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pCMFlag_hsIL1B (#RDB06666)

Expression vector of human IL1B/IL-1 beta.

Alternative name SET-0196_11
Clone info. Expression vector of human IL1B/IL-1 beta. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment Derived from Sugano CAS08366. PCR cloning, forward primer: gcagaagtacctgagctcgccag; reverse primer: ctttaggaagacacaaattgcatgg. Nucleotide substitution T673C (silent) to NM_000576.
Vector backbone pCMV_S-FLAG (RDB05956) (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human IL1B/IL-1 beta cDNA
Depositor|Developer DNA Bank, |
 
Sequence (full) RDB06666hts01.seq checkAssembled from experimentally sequenced data.
Remarks, protocol and/or map (pdf) RDB06666.pdf
Publication Unpublished bioresource
Test sheet RDB13520_A5Iop1-1.pdf 
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL and cite Gene 200, 149-156, 1997 in any publication.
提供案内 (日本国内) [open/close]

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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 発表に際し、理研BRCから入手したことを明記し、指定する文献(Gene, 200, 149-156 (1997))を引用すること。

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RDB06666 pCMFlag_hsIL1B DNA solution

How to cite this biological resource

Materials & Methods section:

The pCMFlag_hsIL1B was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06666).

Reference section:

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

user_report Yokoyama, C., Interleukin-8 enhances the effect of colchicine on cell death. Biochem. Biophys. Res. Commun. 485 (1): 89-94 (2017). PMID 28189686. [link to RRC of NBRP]
user_report Suzuki, S., Shigella type III secretion protein MxiI is recognized by Naip2 to induce Nlrc4 inflammasome activation independently of Pkc delta. PLoS Pathog., 10 (2): e1003926 (2014). PMID 24516390. [link to RRC of NBRP]