Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCMFlag_hsIL1B

Expression vector of human IL1B/IL-1 beta.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL and cite Gene 200, 149-156, 1997 in any publication.
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	発表に際し、理研BRCから入手したことを明記し、指定する文献(Gene, 200, 149-156 (1997))を引用すること。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13520_A5Iop1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB13520_A5Ioa.seq
>06666_13520_A5Io_CMV-Forward_D12_12.ab1     1 TTTACACTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA    61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA   121 CTCACTATAG GGAGACCCAA GCTGAATTCA CCATGGACTA CAAGGACGAT GACGATAAGG   181 CGGCCGCAGA AGTACCTGAG CTCGCCAGTG AAATGATGGC TTATTACAGT GGCAATGAGG   241 ATGACTTGTT CTTTGAAGCT GATGGCCCTA AACAGATGAA GTGCTCCTTC CAGGACCTGG   301 ACCTCTGCCC TCTGGATGGC GGCATCCAGC TACGAATCTC CGACCACCAC TACAGCAAGG   361 GCTTCAGGCA GGCCGCGTCA GTTGTTGTGG CCATGGACAA GCTGAGGAAG ATGCTGGTTC   421 CCTGCCCACA GACCTTCCAG GAGAATGACC TGAGCACCTT CTTTCCCTTC ATCTTTGAAG   481 AAGAACCTAT CTTCTTCGAC ACATGGGATA ACGAGGCTTA TGTGCACGAT GCACCTGTAC   541 GATCACTGAA CTGCACGCTC CGGGACTCAC AGCAAAAAAG CTTGGTGATG TCTGGTCCAT   601 ATGAACTGAA AGCTCTCCAC CTCCAGGGAC AGGATATGGA GCAACAAGTG GTGTTCTCCA   661 TGTCCTTTGT ACAAGGAGAA GAAAGTAATG ACAAAATACC TGTGGCCTTG GGCCTCAAGG   721 AAAAGAATCT GTACCTGTCC TGCGTGTTGA AAGATGATAA GCCCACCCTA CAGCTGGAGA   781 GTGTAGATCC CAAAAATTAC CCAAAGAAGA GATGGAAAAG CGATTTGTCT TCAACAGATA   841 GAAATCAATA ACAAGCTGGA ATTTGAGTCT GCCCAGTTCC CCAACTGGTA CATCAGCACT   901 CTCAGCAGAA ACATGCCCGT CTTCTGGAGG ACAAGCGCAG ATATACTGAC TCACATGCAT   961 TTGTGTCTCT AAAGCCGCTC GAGCATGCAT CTAGAGACTA TTCTATATGT CACTTAATGC  1021 TAAGCTCGCT GATCAGCTCG ACTGTGCTTC AGTGCCAGCA ATCGTGAGGC AGCGTGCATC 1081 TGACTGAAGT CAGCCAACTG TCGTC //
Primer: BGH_rev Sequence file: RDB13520_A5Iob.seq
>06666_13520_A5Io_BGHrev_E07_13.ab1     1 GATTGGCATA GGGTGACTAT AGAATAGGGC CCTCTAGATG CATGCTCGAG CGGCCCTTTA    61 GGAAGACACA AATTGCATGG TGAAGTCAGT TATATCCTGG CCGCCTTTGG TCCCTCCCAG   121 GAAGACGGGC ATGTTTTCTG CTTGAGAGGT GCTGATGTAC CAGTTGGGGA ACTGGGCAGA   181 CTCAAATTCC AGCTTGTTAT TGATTTCTAT CTTGTTGAAG ACAAATCGCT TTTCCATCTT   241 CTTCTTTGGG TAATTTTTGG GATCTACACT CTCCAGCTGT AGGGTGGGCT TATCATCTTT   301 CAACACGCAG GACAGGTACA GATTCTTTTC CTTGAGGCCC AAGGCCACAG GTATTTTGTC   361 ATTACTTTCT TCTCCTTGTA CAAAGGACAT GGAGAACACC ACTTGTTGCT CCATATCCTG   421 TCCCTGGAGG TGGAGAGCTT TCAGTTCATA TGGACCAGAC ATCACCAAGC TTTTTTGCTG   481 TGAGTCCCGG AGCGTGCAGT TCAGTGATCG TACAGGTGCA TCGTGCACAT AAGCCTCGTT   541 ATCCCATGTG TCGAAGAAGA TAGGTTCTTC TTCAAAGATG AAGGGAAAGA AGGTGCTCAG   601 GTCATTCTCC TGGAAGGTCT GTGGGCAGGG AACCAGCATC TTCCTCAGCT TGTCCATGGC   661 CACAACAACT GACGCGGCCT GCCTGAAGCC CTTGCTGTAG TGGTGGTCGG AGATTCGTAG   721 CTGGATGCCG CCATCCAGAG GGCAGAGGTC CAGGTCCTGG AAGGAGCACT TCATCTGTTT   781 AGGGCCATCA GCTTCAAAGA ACAAGTCATC CTCATTGCCA CTGTAATAAG CCATCATTTC   841 ACTGGCGAGC TCAGGTACTT CTGCGGCCGC CTTATCGTCA TCGTCCTTGT AGTCCATGGT   901 GAATTCAGCT TGGGTCTCCC TATAGTGAGT CGTATTAATT TCGATAAGCC AGTAAGCAGT   961 GGGTTTCTCT AGTTTAGCCA GAGAGCTCTG CTTATATAGA CCCTCCCACC CGTACACGCC  1021 CTACGGCCCA TTTGGCGTCA ATGGGCCGAA GTGGTTACGA CATTTTGCAA GTCCGATTGA 1081 ATTTTTGGCT GACCGACAAC AAAACGT //
Primer: SV40pro_F_V2 Sequence file: RDB13520_A5Ioc.seq
>06666_13520_A5Io_SV40pro_F_V2_F12_18.ab1     1 GTCCGAACTT TTTTTGGAGC TAGGCTTTTG CAAAAGCTCC CGGGAGCTTG GATATCCATT    61 TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG   121 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG   181 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT   241 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA   301 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG   361 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT   421 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT   481 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG   541 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA   601 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC   661 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC   721 GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT   781 ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC   841 GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA   901 CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT   961 CCACCGCCGC CTTCTATGAA AAGTTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG  1021 ATGATCTCAG CGCGGGGATC TCATGCTGGA GTCTCGCCCA CCCCAAAGTG TTATGCAGCT  1081 TATATGTACA AATAAGGCAA TAGCATCACA TTCACAAATT AAGCATTTTT CACTGACATT 1141 TCTAGGATTG TTTG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.02

GNP_filter3_RDBDEP_html_230501.pl