Resource data sheet
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pCMFlag_hsIL1B (#RDB06666)

Expression vector of human IL1B/IL-1 beta.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)
GenBank Flat File Format open
SnapGene file download
Remarks, protocol and/or map (pdf) RDB06666.pdf
Publication Unpublished bioresource
Test sheet Data Sheet open 
 
Alternative name SET-0196_11
Clone info. Expression vector of human IL1B/IL-1 beta. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment Derived from Sugano CAS08366. PCR cloning, forward primer: gcagaagtacctgagctcgccag; reverse primer: ctttaggaagacacaaattgcatgg. Nucleotide substitution T673C (silent) to NM_000576.
Vector backbone pCMV_S-FLAG (RDB05956) (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Growth conditions 37C, LB+Amp
Gene/insert name Human IL1B/IL-1 beta cDNA
Depositor|Developer DNA Bank, |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL and cite Gene 200, 149-156, 1997 in any publication.
Additional terms and conditions:
Regrding pRc/CMV:
a) The modified pRc/CMV, and their descendants (collectively, LIFE MATERIALS), should be used for pre-clinical/non-human, research use only.
b) LIFE MATERIALS shall not be used for any commercial purposes.
c) LIFE MATERIALS shall not be transferred beyond the specified recipient.
d) Recipient must contact Thermo Fisher Scientific Inc. at outlicense@thermofisher.com for any desired use of LIFE MATERIALS beyond the scope of a) to c) above.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 発表に際し、理研BRCから入手したことを明記し、指定する文献(Gene, 200, 149-156 (1997))を引用すること。
付加的使用条件:
pRc/CMVについて:
a) The modified pRc/CMV, and their descendants (collectively, LIFE MATERIALS), should be used for pre-clinical/non-human, research use only.
b) LIFE MATERIALS shall not be used for any commercial purposes.
c) LIFE MATERIALS shall not be transferred beyond the specified recipient.
d) Recipient must contact Thermo Fisher Scientific Inc. at outlicense@thermofisher.com for any desired use of LIFE MATERIALS beyond the scope of a) to c) above.

Catalog # Resource name Shipping form Fee
RDB06666 pCMFlag_hsIL1B DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge


How to cite this biological resource

Materials & Methods section:

The pCMFlag_hsIL1B was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06666).

Reference section:

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

user_report Yokoyama, C., Interleukin-8 enhances the effect of colchicine on cell death. Biochem. Biophys. Res. Commun. 485 (1): 89-94 (2017). PMID 28189686. [PubMed] [Article] [RRC of NBRP]
user_report Suzuki, S., Shigella type III secretion protein MxiI is recognized by Naip2 to induce Nlrc4 inflammasome activation independently of Pkc delta. PLoS Pathog., 10 (2): e1003926 (2014). PMID 24516390. [PubMed] [Article] [RRC of NBRP]