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pFLAG-CMV-2-WT-human Akt3 (#RDB06616)

Mammaian expression vector encoding human WT-Akt3

Clone info. Mammaian expression vector encoding human WT-Akt3.
Comment Expression was confirmed with Western blot analysis using anti-Akt and anti-FLAG antibodies by the depositor.
Vector backbone pFLAG-CMV-2 (Plasmid)
Size of vector backbone 4.7 kbp
Selectable markers Amp^r
Gene/insert name Human AKT3 cDNA
Depositor|Developer Fujita, Naoya |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Tokuda, E., Cancer Res., 67, 9666-9676 (2007))
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Tokuda, E., Cancer Res., 67, 9666-9676 (2007))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06616 pFLAG-CMV-2-WT-human Akt3 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pFLAG-CMV-2-WT-human Akt3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06616).

Reference section:

Tokuda, E., Fujita, N., Oh-hara, T., Sato, S., Kurata, A., Katayama, R., Itoh, T., Takenawa, T., Miyazono, K., Tsuruo, T., Casein kinase 2-interacting protein-1, a novel Akt pleckstrin homology domain-interacting protein, down-regulates PI3K/Akt signaling and suppresses tumor growth in vivo. Cancer Res., 67, 9666-9676 (2007). PMID 17942896. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12821_A4Gg.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB12821_A4Gga.seq check
>06616-12821_A4Gg_CMV-Foward_G07_19.ab1
    1 ATTAAAAAAC CCCCCGCCCC CCGGTTTGAC GCCAATGGGC GGTAGGCGTT GGTACGGGTG
   61 GGAGGTCTAT ATAAGCAGAG CTCGTTTAGT GAACCGTCAG AATTGATCTA CCATGGACTA
  121 CAAAGACGAT GACGACAGCT TGCGGCCGCG AATTCGGCTT TGAGCGATGT TACCATTGTG
  181 AAAGAAGGTT GGGTTCAGAA GAGGGGAGAA TATATAAAAA ACTGGAGGCC AAGATACTTC
  241 CTTTTGAAGA CAGATGGCTC ATTCATAGGA TATAAAGAGA AACCTCAAGA TGTGGATTTA
  301 CCTTATCCCC TCAACAACTT TTCAGTGGCA AAATGCCAGT TAATGAAAAC AGAACGACCA
  361 AAGCCAAACA CATTTATAAT CAGATGTCTC CAGTGGACTA CTGTTATAGA GAGAACATTT
  421 CATGTAGATA CTCCAGAGGA AAGGGAAGAA TGGACAGAAG CTATCCAGGC TGTAGCAGAC
  481 AGACTGCAGA GGCAAGAAGA GGAGAGAATG AATTGTAGTC CAACTTCACA AATTGATAAT
  541 ATAGGAGAGG AAGAGATGGA TGCCTCTACA ACCCATCATA AAAGAAAGAC AATGAATGAT
  601 TTTGACTATT TGAAACTACT AGGTAAAGGC ACTTTTGGGA AAGTTATTTT GGTTCGAGAG
  661 AAGGCAAGTG GAAAATACTA TGCTATGAAG ATTCTGAAGA AAGAAGTCAT TATTGCAAAG
  721 GATGAAGTGG CACACACTCC TAACCTGAAA GCAGAGTATT AAAGAACACT AGACATCCCT
  781 TTTTAACATC CTTGAAATAT TCCTTTCCAG ACAAAAGACC GTTTGTGTTT TGTGATGGAA
  841 TATGTTAATG GGGGGGCGAG CTGTTTTTTC CATTTTGTCG AGAGAGCGGG GTGTTCTCTG
  901 AAGGACCGCA CACCGTTTTC TAATGGGTGC AGAAAATTGT CTCTGCCCTT GGACTATTCT
  961 ACAATTCCGG AAAGATTGTG TACCGGTGAT CTCCAGTTGG GAGAATCTAA TGCTGGGACA
1021 AGCACGCGCC CACACTAACA ACTT
//
Primer: C-CMV24
Sequence file: RDB12821_A4Ggb.seq check
>06616_12821_A4Gg_C-CMV24_B10_04.ab1
    1 GTGAAGGCAC TTCAGGGCCA GGAGAGGCCT GGGGAGGGGT CACAGGGATG CCACCCGGGA
   61 TCCTCTAGAG TCGACTGGTG CCGATATCAG ATCTATCGAT GAATTCGGCT TGACTTATTC
  121 TCGTCCACTT GCAGAGTAGG AAAATTGAGG GAAATGCGGC CGCCTCTCAT TGTCCATGCA
  181 GTCCATACCA TCCTCATCAT ATTTTTCAGG TGGGGTTATT GTAATAGTCT GAGCTGTAAA
  241 TTCTTCATCA AAATATCTAG TATCTGTCTC ACATGTTACT TGAGGTTTAA AAGGAGGTAC
  301 AAGCTTTTTA TCATATACAT CTTGCCAGTT TACTCCAGAG AAAAAACTGT GTCTCATAAT
  361 TTCTTTTGCA TCATCTGGTC CTCCACCAAG GCGTTTATTT GGATCCTTTA TCAAGAGCCC
  421 TGAAAGCAAT GATTTTGCAT CTGAAGAGAG TGTTCGAGGA AATTTAATGT CTTCCATTAA
  481 TATTAATTCA AAAAGTTTCT CATGGTCCTG GTTGTAGAAA GGTAACCTCC CACACATCAT
  541 TTCATACATG ACAACCCCTA GGCCCCACCA GTCTACTGCT CGGCCATAGT CATTATCTTC
  601 TAACACCTCT GGTGCCAGAT ATTCTGGAGT GCCACAGAAT GTCTTCATGG TGGCTGCATC
  661 TGTGATCCCT TCTTTGCAAA GTCCAAAATC TGTAATTTTT ATGTGGCCAT CTTTGTCCAG
  721 CATTAGATTC TCCAACTTGA GATCACGGTA CACAATCTTT CCGGAATGTA GATAGTCCAA
  781 GGCAGAGACA ATTTCTGCAC CATAGAAACG TGTGCGGTCC TCACAGAACA CCCGCTCTCT
  841 CGACAAATGG AAAAACAGCT CGCCCCCATT AACATATTCC ATCACAAAAC ACAAACGGTC
  901 TTTTGTCTGG AAGAATATTT CAAGGATGTT AAAAAGGGAT GTCTAGTGTT CTTTATACTC
  961 TGCTTCAGTT AGAGTGTGTG CCACTTCATC CTTGCATATG ACTTCTTTCT CAGAATCCTC
 1021 ATAGCATAGT AATTTCCACT GGCTCTCTCG ACAAAATACC TTCCAATGCG TACCAGAGTC
1081 AAGTCAATCA TCATGCTCTA GATGGTTCAG CATCAACTCT CGTCTCTAAT A
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tokuda, E., Casein kinase 2-interacting protein-1, a novel Akt pleckstrin homology domain-interacting protein, down-regulates PI3K/Akt signaling and suppresses tumor growth in vivo. Cancer Res., 67, 9666-9676 (2007). PMID 17942896. [link to RRC of NBRP]