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pFLAG-CMV-2-WT-human Akt3

Mammaian expression vector encoding human WT-Akt3

Catalog number RDB06616
Resource name pFLAG-CMV-2-WT-human Akt3
Clone info. Mammaian expression vector encoding human WT-Akt3.
Expression was confirmed with Western blot analysis using anti-Akt and anti-FLAG antibodies by the depositor.
Vector backbone pFLAG-CMV-2 (Plasmid)
Size of vector backbone 4.7 kbp
Selectable markers Amp^r
Gene/insert name Human AKT3 cDNA
Depositor Fujita, Naoya |
 
Other clones in our bank human AKT3 (NCBI Gene 10000) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Tokuda, E., Cancer Res., 67, 9666-9676 (2007))
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RDB06616 pFLAG-CMV-2-WT-human Akt3 DNA solution

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References and tips

Electronic file

Featured content

References

original Tokuda, E., Casein kinase 2-interacting protein-1, a novel Akt pleckstrin homology domain-interacting protein, down-regulates PI3K/Akt signaling and suppresses tumor growth in vivo. Cancer Res., 67, 9666-9676 (2007). PMID 17942896.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12821_A4Gg.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB12821_A4Gga.seq
>06616-12821_A4Gg_CMV-Foward_G07_19.ab1
    1 ATTAAAAAAC CCCCCGCCCC CCGGTTTGAC GCCAATGGGC GGTAGGCGTT GGTACGGGTG
   61 GGAGGTCTAT ATAAGCAGAG CTCGTTTAGT GAACCGTCAG AATTGATCTA CCATGGACTA
  121 CAAAGACGAT GACGACAGCT TGCGGCCGCG AATTCGGCTT TGAGCGATGT TACCATTGTG
  181 AAAGAAGGTT GGGTTCAGAA GAGGGGAGAA TATATAAAAA ACTGGAGGCC AAGATACTTC
  241 CTTTTGAAGA CAGATGGCTC ATTCATAGGA TATAAAGAGA AACCTCAAGA TGTGGATTTA
  301 CCTTATCCCC TCAACAACTT TTCAGTGGCA AAATGCCAGT TAATGAAAAC AGAACGACCA
  361 AAGCCAAACA CATTTATAAT CAGATGTCTC CAGTGGACTA CTGTTATAGA GAGAACATTT
  421 CATGTAGATA CTCCAGAGGA AAGGGAAGAA TGGACAGAAG CTATCCAGGC TGTAGCAGAC
  481 AGACTGCAGA GGCAAGAAGA GGAGAGAATG AATTGTAGTC CAACTTCACA AATTGATAAT
  541 ATAGGAGAGG AAGAGATGGA TGCCTCTACA ACCCATCATA AAAGAAAGAC AATGAATGAT
  601 TTTGACTATT TGAAACTACT AGGTAAAGGC ACTTTTGGGA AAGTTATTTT GGTTCGAGAG
  661 AAGGCAAGTG GAAAATACTA TGCTATGAAG ATTCTGAAGA AAGAAGTCAT TATTGCAAAG
  721 GATGAAGTGG CACACACTCC TAACCTGAAA GCAGAGTATT AAAGAACACT AGACATCCCT
  781 TTTTAACATC CTTGAAATAT TCCTTTCCAG ACAAAAGACC GTTTGTGTTT TGTGATGGAA
  841 TATGTTAATG GGGGGGCGAG CTGTTTTTTC CATTTTGTCG AGAGAGCGGG GTGTTCTCTG
  901 AAGGACCGCA CACCGTTTTC TAATGGGTGC AGAAAATTGT CTCTGCCCTT GGACTATTCT
  961 ACAATTCCGG AAAGATTGTG TACCGGTGAT CTCCAGTTGG GAGAATCTAA TGCTGGGACA
1021 AGCACGCGCC CACACTAACA ACTT
//
Primer: C-CMV24 RDB12821_A4Ggb.seq
>06616_12821_A4Gg_C-CMV24_B10_04.ab1
    1 GTGAAGGCAC TTCAGGGCCA GGAGAGGCCT GGGGAGGGGT CACAGGGATG CCACCCGGGA
   61 TCCTCTAGAG TCGACTGGTG CCGATATCAG ATCTATCGAT GAATTCGGCT TGACTTATTC
  121 TCGTCCACTT GCAGAGTAGG AAAATTGAGG GAAATGCGGC CGCCTCTCAT TGTCCATGCA
  181 GTCCATACCA TCCTCATCAT ATTTTTCAGG TGGGGTTATT GTAATAGTCT GAGCTGTAAA
  241 TTCTTCATCA AAATATCTAG TATCTGTCTC ACATGTTACT TGAGGTTTAA AAGGAGGTAC
  301 AAGCTTTTTA TCATATACAT CTTGCCAGTT TACTCCAGAG AAAAAACTGT GTCTCATAAT
  361 TTCTTTTGCA TCATCTGGTC CTCCACCAAG GCGTTTATTT GGATCCTTTA TCAAGAGCCC
  421 TGAAAGCAAT GATTTTGCAT CTGAAGAGAG TGTTCGAGGA AATTTAATGT CTTCCATTAA
  481 TATTAATTCA AAAAGTTTCT CATGGTCCTG GTTGTAGAAA GGTAACCTCC CACACATCAT
  541 TTCATACATG ACAACCCCTA GGCCCCACCA GTCTACTGCT CGGCCATAGT CATTATCTTC
  601 TAACACCTCT GGTGCCAGAT ATTCTGGAGT GCCACAGAAT GTCTTCATGG TGGCTGCATC
  661 TGTGATCCCT TCTTTGCAAA GTCCAAAATC TGTAATTTTT ATGTGGCCAT CTTTGTCCAG
  721 CATTAGATTC TCCAACTTGA GATCACGGTA CACAATCTTT CCGGAATGTA GATAGTCCAA
  781 GGCAGAGACA ATTTCTGCAC CATAGAAACG TGTGCGGTCC TCACAGAACA CCCGCTCTCT
  841 CGACAAATGG AAAAACAGCT CGCCCCCATT AACATATTCC ATCACAAAAC ACAAACGGTC
  901 TTTTGTCTGG AAGAATATTT CAAGGATGTT AAAAAGGGAT GTCTAGTGTT CTTTATACTC
  961 TGCTTCAGTT AGAGTGTGTG CCACTTCATC CTTGCATATG ACTTCTTTCT CAGAATCCTC
 1021 ATAGCATAGT AATTTCCACT GGCTCTCTCG ACAAAATACC TTCCAATGCG TACCAGAGTC
1081 AAGTCAATCA TCATGCTCTA GATGGTTCAG CATCAACTCT CGTCTCTAAT A
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Tokuda, E., Casein kinase 2-interacting protein-1, a novel Akt pleckstrin homology domain-interacting protein, down-regulates PI3K/Akt signaling and suppresses tumor growth in vivo. Cancer Res., 67, 9666-9676 (2007). PMID 17942896.

2018.12.11

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