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pFLAG-CMV-2-WT-human Akt2 (#RDB06615)

Mammaian expression vector encoding human WT-Akt2

Clone info. Mammaian expression vector encoding human WT-Akt2.
Comment Expression was confirmed with Western blot analysis using anti-Akt and anti-FLAG antibodies by the depositor.
Vector backbone pFLAG-CMV-2 (Plasmid)
Size of vector backbone 4.7 kbp
Selectable markers Amp^r
Gene/insert name Human AKT2 cDNA
Depositor|Developer Fujita, Naoya |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Tokuda, E., Cancer Res., 67, 9666-9676 (2007))
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Tokuda, E., Cancer Res., 67, 9666-9676 (2007))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06615 pFLAG-CMV-2-WT-human Akt2 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pFLAG-CMV-2-WT-human Akt2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06615).

Reference section:

Tokuda, E., Fujita, N., Oh-hara, T., Sato, S., Kurata, A., Katayama, R., Itoh, T., Takenawa, T., Miyazono, K., Tsuruo, T., Casein kinase 2-interacting protein-1, a novel Akt pleckstrin homology domain-interacting protein, down-regulates PI3K/Akt signaling and suppresses tumor growth in vivo. Cancer Res., 67, 9666-9676 (2007). PMID 17942896. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14149_A6Drp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB14149_A6Dra.seq check
>06615_14149_A6Dr_CMV-Forward_A08_02.ab1
    1 AGTTATACCC CGGCCCGTTG ACGCAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
   61 AAGCAGAGCT CGTTTAGTGA ACCGTCAGAA TTGATCTACC ATGGACTACA AAGACGATGA
  121 CGACAAGCTT GCGGCCGCGA ATTCGGCTTT GAATGAGGTG TCTGTCATCA AAGAAGGCTG
  181 GCTCCACAAG CGTGGTGAAT ACATCAAGAC CTGGAGGCCA CGGTACTTCC TGCTGAAGAG
  241 CGACGGCTCC TTCATTGGGT ACAAGGAGAG GCCCGAGGCC CCTGATCAGA CTCTACCCCC
  301 CTTAAACAAC TTCTCCGTAG CAGAATGCCA GCTGATGAAG ACCGAGAGGC CGCGACCCAA
  361 CACCTTTGTC ATACGCTGCC TGCAGTGGAC CACAGTCATC GAGAGGACCT TCCACGTGGA
  421 TTCTCCAGAC GAGAGGGAGG AGTGGATGCG GGCCATCCAG ATGGTCGCCA ACAGCCTCAA
  481 GCAGCGGGCC CCAGGCGAGG ACCCCATGGA CTACAAGTGT GGCTCCCCCA GTGACTCCTC
  541 CACGACTGAG GAGATGGAAG TGGCGGTCAG CAAGGCACGG GCTAAAGTGA CCATGAATGA
  601 CTTCGACTAT CTCAAACTCC TTGGCAAGGG AACCTTTGGC AAAGTCATCC TGGTGCGGGA
  661 GAAGGCCACT GGCCGCTACT ACGCCATGAA GATCCTGCGG AAGGAAGTCA TCATTGCCAA
  721 GGATGAAGTC GCTCACACAG TCACCGAGAG CCGGGTCCTC CAGAACACCA GGCACCCGTT
  781 CCTCACTGCG CTGAAGTATG CCTTCCAGAC CCACGACCGC CTGTGCTTTG TGATGGAGTA
  841 TGCCAACGGG GGGGTGAGCT GTTCTTCCAC CTGTCCCCGG GAGCGTGTCT TCACAGAGGG
  901 AGCGGGCCCC GGTTTTATGG TGCCAGAGAT TGTCCTCGGC CTCCTTGAGT ACTTGGCACT
  961 CGCGGGACGG TGGTATACCG GCGACATCAG CTGGGAAAAC CTTCATGCTG GACAAAGGAT
 1021 GGCCACATCA AGAATCACTT GGACCTTTGC CTCTGGCAAG GAGGGCATTC CAGGTGAACG
//
Primer: hGHpolyA-R
Sequence file: RDB14149_A6Drb.seq check
>06615_14149_A6Dr_hGHpolyA_R_B08_05.ab1
    1 CTAACCCCCA TCACCCCATA ATATTATAGA AGGACACCTA GTCAGACAAA ATGATGCAAC
   61 TTAATTTTAT TAGGACAAGG CTGGTGGGCA CTGGAGTGGC AACTTCCAGG GCCAGGAGAG
  121 GCACTGGGGA GGGGTCACAG GGATGCCACC CGGGATCCTC TAGAGTCGAC TGGTACCGAT
  181 ATCAGATCTA TCGATGAATT CGGCTTGACT GCTCACTCGC GGATGCTGGC CGAGTAGGAG
  241 AACTGGGGGA AGTGGGTCCG CTGGTCCAGC TCCAGTAAGC CCAGGCTGTC ATAGCGGTCA
  301 GGGGGTGTGA TTGTGATGGA CTGGGCGGTA AATTCATCAT CGAAGTACCT TGTGTCGACC
  361 TCGGACGTGA CCTGAGGTTT GAAGGGTGGC AGGAGCTTCT TCTGGACCAC GTCCTGCCAG
  421 TTGATGCTGA GGAAGAACCT GTGCTCCATG ACCTCCTTGG CATCGCTGGG CCCCCCACCA
  481 AGCCTCTGCT TGGGGTCCTT CTTAAGCAGC CCAGCAAGCA GGGACTTGGC CTCGGGGCTG
  541 AGCGTGCGCG GGAAGCGGAT CTCTTCCATG AGGATGAGCT CGAAGAGGCG CTCGTGGTCC
  601 TGGTTGTAGA AGGGCAGGCG GCCGCACATC ATCTCGTACA TGACCACACC CAGCCCCCAC
  661 CAGTCCACGG CCCGGCCATA GTCATTGTCC TCCAGCACCT CAGGCGCCAG GTACTCCGGG
  721 GTCCCACAGA AGGTTTTCAT GGTGGCCCCG TCACTGATGC CCTCTTTGCA GAGGCCAAAG
  781 TCAGTGATCT TGATGTGGCC ATCTTTGTCC AGCATGAGGT TTTCCAGCTT GATGTCGCGG
  841 TATACCACGT CCCGCGAGTG CAAGTACTCA AGAGCCGAGA CAATCTCTGC ACCATAAAAC
  901 CGGGCCCGCT CCTCTGTGAA GACACGCTCC CGGGACAGGT GGAAGAACAG CTCACCCCCC
  961 GTTTGGCATA CTCCATCACA AGCACAAGGC GGTCGTGGGG TCTGGAGGCA TACTTCAGCG
 1021 GCAGTGAGGA CGGCTGGCCC TTGGTTGTTC TGGTAGACCG GCTCTCGGTG ACTGATGATG
1081 GAGCGAACGT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tokuda, E., Casein kinase 2-interacting protein-1, a novel Akt pleckstrin homology domain-interacting protein, down-regulates PI3K/Akt signaling and suppresses tumor growth in vivo. Cancer Res., 67, 9666-9676 (2007). PMID 17942896. [link to RRC of NBRP]
user_report Kikkawa, Y., Internalization of CD239 highly expressed in breast cancer cells: a potential antigen for antibody-drug conjugates. Sci. Rep. 8 (1): 6612 (2018). PMID 29700410. [link to RRC of NBRP]