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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pFLAG-CMV-2-WT-human Akt2

Mammaian expression vector encoding human WT-Akt2

Catalog number RDB06615
Resource name pFLAG-CMV-2-WT-human Akt2
Clone info. Mammaian expression vector encoding human WT-Akt2.
Comment Expression was confirmed with Western blot analysis using anti-Akt and anti-FLAG antibodies by the depositor.
Vector backbone pFLAG-CMV-2 (Plasmid)
Size of vector backbone 4.7 kbp
Selectable markers Amp^r
Gene/insert name Human AKT2 cDNA
Depositor|Developer Fujita, Naoya |
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External Database
Human AKT2

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Tokuda, E., Cancer Res., 67, 9666-9676 (2007))
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Tokuda, E., Cancer Res., 67, 9666-9676 (2007))。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB06615 pFLAG-CMV-2-WT-human Akt2 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Tokuda, E., Casein kinase 2-interacting protein-1, a novel Akt pleckstrin homology domain-interacting protein, down-regulates PI3K/Akt signaling and suppresses tumor growth in vivo. Cancer Res., 67, 9666-9676 (2007). PMID 17942896.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14149_A6Drp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB14149_A6Dra.seq check
          >06615_14149_A6Dr_CMV-Forward_A08_02.ab1
              1 AGTTATACCC CGGCCCGTTG ACGCAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
             61 AAGCAGAGCT CGTTTAGTGA ACCGTCAGAA TTGATCTACC ATGGACTACA AAGACGATGA
            121 CGACAAGCTT GCGGCCGCGA ATTCGGCTTT GAATGAGGTG TCTGTCATCA AAGAAGGCTG
            181 GCTCCACAAG CGTGGTGAAT ACATCAAGAC CTGGAGGCCA CGGTACTTCC TGCTGAAGAG
            241 CGACGGCTCC TTCATTGGGT ACAAGGAGAG GCCCGAGGCC CCTGATCAGA CTCTACCCCC
            301 CTTAAACAAC TTCTCCGTAG CAGAATGCCA GCTGATGAAG ACCGAGAGGC CGCGACCCAA
            361 CACCTTTGTC ATACGCTGCC TGCAGTGGAC CACAGTCATC GAGAGGACCT TCCACGTGGA
            421 TTCTCCAGAC GAGAGGGAGG AGTGGATGCG GGCCATCCAG ATGGTCGCCA ACAGCCTCAA
            481 GCAGCGGGCC CCAGGCGAGG ACCCCATGGA CTACAAGTGT GGCTCCCCCA GTGACTCCTC
            541 CACGACTGAG GAGATGGAAG TGGCGGTCAG CAAGGCACGG GCTAAAGTGA CCATGAATGA
            601 CTTCGACTAT CTCAAACTCC TTGGCAAGGG AACCTTTGGC AAAGTCATCC TGGTGCGGGA
            661 GAAGGCCACT GGCCGCTACT ACGCCATGAA GATCCTGCGG AAGGAAGTCA TCATTGCCAA
            721 GGATGAAGTC GCTCACACAG TCACCGAGAG CCGGGTCCTC CAGAACACCA GGCACCCGTT
            781 CCTCACTGCG CTGAAGTATG CCTTCCAGAC CCACGACCGC CTGTGCTTTG TGATGGAGTA
            841 TGCCAACGGG GGGGTGAGCT GTTCTTCCAC CTGTCCCCGG GAGCGTGTCT TCACAGAGGG
            901 AGCGGGCCCC GGTTTTATGG TGCCAGAGAT TGTCCTCGGC CTCCTTGAGT ACTTGGCACT
            961 CGCGGGACGG TGGTATACCG GCGACATCAG CTGGGAAAAC CTTCATGCTG GACAAAGGAT
           1021 GGCCACATCA AGAATCACTT GGACCTTTGC CTCTGGCAAG GAGGGCATTC CAGGTGAACG
          //
          Primer: hGHpolyA-R
          Sequence file: RDB14149_A6Drb.seq check
          >06615_14149_A6Dr_hGHpolyA_R_B08_05.ab1
              1 CTAACCCCCA TCACCCCATA ATATTATAGA AGGACACCTA GTCAGACAAA ATGATGCAAC
             61 TTAATTTTAT TAGGACAAGG CTGGTGGGCA CTGGAGTGGC AACTTCCAGG GCCAGGAGAG
            121 GCACTGGGGA GGGGTCACAG GGATGCCACC CGGGATCCTC TAGAGTCGAC TGGTACCGAT
            181 ATCAGATCTA TCGATGAATT CGGCTTGACT GCTCACTCGC GGATGCTGGC CGAGTAGGAG
            241 AACTGGGGGA AGTGGGTCCG CTGGTCCAGC TCCAGTAAGC CCAGGCTGTC ATAGCGGTCA
            301 GGGGGTGTGA TTGTGATGGA CTGGGCGGTA AATTCATCAT CGAAGTACCT TGTGTCGACC
            361 TCGGACGTGA CCTGAGGTTT GAAGGGTGGC AGGAGCTTCT TCTGGACCAC GTCCTGCCAG
            421 TTGATGCTGA GGAAGAACCT GTGCTCCATG ACCTCCTTGG CATCGCTGGG CCCCCCACCA
            481 AGCCTCTGCT TGGGGTCCTT CTTAAGCAGC CCAGCAAGCA GGGACTTGGC CTCGGGGCTG
            541 AGCGTGCGCG GGAAGCGGAT CTCTTCCATG AGGATGAGCT CGAAGAGGCG CTCGTGGTCC
            601 TGGTTGTAGA AGGGCAGGCG GCCGCACATC ATCTCGTACA TGACCACACC CAGCCCCCAC
            661 CAGTCCACGG CCCGGCCATA GTCATTGTCC TCCAGCACCT CAGGCGCCAG GTACTCCGGG
            721 GTCCCACAGA AGGTTTTCAT GGTGGCCCCG TCACTGATGC CCTCTTTGCA GAGGCCAAAG
            781 TCAGTGATCT TGATGTGGCC ATCTTTGTCC AGCATGAGGT TTTCCAGCTT GATGTCGCGG
            841 TATACCACGT CCCGCGAGTG CAAGTACTCA AGAGCCGAGA CAATCTCTGC ACCATAAAAC
            901 CGGGCCCGCT CCTCTGTGAA GACACGCTCC CGGGACAGGT GGAAGAACAG CTCACCCCCC
            961 GTTTGGCATA CTCCATCACA AGCACAAGGC GGTCGTGGGG TCTGGAGGCA TACTTCAGCG
           1021 GCAGTGAGGA CGGCTGGCCC TTGGTTGTTC TGGTAGACCG GCTCTCGGTG ACTGATGATG
          1081 GAGCGAACGT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Tokuda, E., Casein kinase 2-interacting protein-1, a novel Akt pleckstrin homology domain-interacting protein, down-regulates PI3K/Akt signaling and suppresses tumor growth in vivo. Cancer Res., 67, 9666-9676 (2007). PMID 17942896.
          user_report Kikkawa, Y., Internalization of CD239 highly expressed in breast cancer cells: a potential antigen for antibody-drug conjugates. Sci. Rep. 8 (1): 6612 (2018). PMID 29700410.

          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl