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pFLAG-CMV-2-WT-human Akt2

Mammaian expression vector encoding human WT-Akt2

Catalog number RDB06615
Resource name pFLAG-CMV-2-WT-human Akt2
Clone info. Mammaian expression vector encoding human WT-Akt2.
Expression was confirmed with Western blot analysis using anti-Akt and anti-FLAG antibodies by the depositor.
Vector backbone pFLAG-CMV-2 (Plasmid)
Size of vector backbone 4.7 kbp
Selectable markers Amp^r
Gene/insert name Human AKT2 cDNA
Depositor Fujita, Naoya |
 
Other clones in our bank human AKT2 (NCBI Gene 208) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Tokuda, E., Cancer Res., 67, 9666-9676 (2007))
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RDB06615 pFLAG-CMV-2-WT-human Akt2 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Tokuda, E., Casein kinase 2-interacting protein-1, a novel Akt pleckstrin homology domain-interacting protein, down-regulates PI3K/Akt signaling and suppresses tumor growth in vivo. Cancer Res., 67, 9666-9676 (2007). PMID 17942896.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14149_A6Drp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward | Sequence: RDB14149_A6Dra.seq
>06615_14149_A6Dr_CMV-Forward_A08_02.ab1
    1 AGTTATACCC CGGCCCGTTG ACGCAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
   61 AAGCAGAGCT CGTTTAGTGA ACCGTCAGAA TTGATCTACC ATGGACTACA AAGACGATGA
  121 CGACAAGCTT GCGGCCGCGA ATTCGGCTTT GAATGAGGTG TCTGTCATCA AAGAAGGCTG
  181 GCTCCACAAG CGTGGTGAAT ACATCAAGAC CTGGAGGCCA CGGTACTTCC TGCTGAAGAG
  241 CGACGGCTCC TTCATTGGGT ACAAGGAGAG GCCCGAGGCC CCTGATCAGA CTCTACCCCC
  301 CTTAAACAAC TTCTCCGTAG CAGAATGCCA GCTGATGAAG ACCGAGAGGC CGCGACCCAA
  361 CACCTTTGTC ATACGCTGCC TGCAGTGGAC CACAGTCATC GAGAGGACCT TCCACGTGGA
  421 TTCTCCAGAC GAGAGGGAGG AGTGGATGCG GGCCATCCAG ATGGTCGCCA ACAGCCTCAA
  481 GCAGCGGGCC CCAGGCGAGG ACCCCATGGA CTACAAGTGT GGCTCCCCCA GTGACTCCTC
  541 CACGACTGAG GAGATGGAAG TGGCGGTCAG CAAGGCACGG GCTAAAGTGA CCATGAATGA
  601 CTTCGACTAT CTCAAACTCC TTGGCAAGGG AACCTTTGGC AAAGTCATCC TGGTGCGGGA
  661 GAAGGCCACT GGCCGCTACT ACGCCATGAA GATCCTGCGG AAGGAAGTCA TCATTGCCAA
  721 GGATGAAGTC GCTCACACAG TCACCGAGAG CCGGGTCCTC CAGAACACCA GGCACCCGTT
  781 CCTCACTGCG CTGAAGTATG CCTTCCAGAC CCACGACCGC CTGTGCTTTG TGATGGAGTA
  841 TGCCAACGGG GGGGTGAGCT GTTCTTCCAC CTGTCCCCGG GAGCGTGTCT TCACAGAGGG
  901 AGCGGGCCCC GGTTTTATGG TGCCAGAGAT TGTCCTCGGC CTCCTTGAGT ACTTGGCACT
  961 CGCGGGACGG TGGTATACCG GCGACATCAG CTGGGAAAAC CTTCATGCTG GACAAAGGAT
 1021 GGCCACATCA AGAATCACTT GGACCTTTGC CTCTGGCAAG GAGGGCATTC CAGGTGAACG
//
Primer: hGHpolyA-R | Sequence: RDB14149_A6Drb.seq
>06615_14149_A6Dr_hGHpolyA_R_B08_05.ab1
    1 CTAACCCCCA TCACCCCATA ATATTATAGA AGGACACCTA GTCAGACAAA ATGATGCAAC
   61 TTAATTTTAT TAGGACAAGG CTGGTGGGCA CTGGAGTGGC AACTTCCAGG GCCAGGAGAG
  121 GCACTGGGGA GGGGTCACAG GGATGCCACC CGGGATCCTC TAGAGTCGAC TGGTACCGAT
  181 ATCAGATCTA TCGATGAATT CGGCTTGACT GCTCACTCGC GGATGCTGGC CGAGTAGGAG
  241 AACTGGGGGA AGTGGGTCCG CTGGTCCAGC TCCAGTAAGC CCAGGCTGTC ATAGCGGTCA
  301 GGGGGTGTGA TTGTGATGGA CTGGGCGGTA AATTCATCAT CGAAGTACCT TGTGTCGACC
  361 TCGGACGTGA CCTGAGGTTT GAAGGGTGGC AGGAGCTTCT TCTGGACCAC GTCCTGCCAG
  421 TTGATGCTGA GGAAGAACCT GTGCTCCATG ACCTCCTTGG CATCGCTGGG CCCCCCACCA
  481 AGCCTCTGCT TGGGGTCCTT CTTAAGCAGC CCAGCAAGCA GGGACTTGGC CTCGGGGCTG
  541 AGCGTGCGCG GGAAGCGGAT CTCTTCCATG AGGATGAGCT CGAAGAGGCG CTCGTGGTCC
  601 TGGTTGTAGA AGGGCAGGCG GCCGCACATC ATCTCGTACA TGACCACACC CAGCCCCCAC
  661 CAGTCCACGG CCCGGCCATA GTCATTGTCC TCCAGCACCT CAGGCGCCAG GTACTCCGGG
  721 GTCCCACAGA AGGTTTTCAT GGTGGCCCCG TCACTGATGC CCTCTTTGCA GAGGCCAAAG
  781 TCAGTGATCT TGATGTGGCC ATCTTTGTCC AGCATGAGGT TTTCCAGCTT GATGTCGCGG
  841 TATACCACGT CCCGCGAGTG CAAGTACTCA AGAGCCGAGA CAATCTCTGC ACCATAAAAC
  901 CGGGCCCGCT CCTCTGTGAA GACACGCTCC CGGGACAGGT GGAAGAACAG CTCACCCCCC
  961 GTTTGGCATA CTCCATCACA AGCACAAGGC GGTCGTGGGG TCTGGAGGCA TACTTCAGCG
 1021 GCAGTGAGGA CGGCTGGCCC TTGGTTGTTC TGGTAGACCG GCTCTCGGTG ACTGATGATG
1081 GAGCGAACGT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Tokuda, E., Casein kinase 2-interacting protein-1, a novel Akt pleckstrin homology domain-interacting protein, down-regulates PI3K/Akt signaling and suppresses tumor growth in vivo. Cancer Res., 67, 9666-9676 (2007). PMID 17942896.
user report Kikkawa, Y., Internalization of CD239 highly expressed in breast cancer cells: a potential antigen for antibody-drug conjugates. Sci. Rep. 8 (1): 6612 (2018). PMID 29700410.

2019.10.05

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