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pCMFlag_hsLIN28 (#RDB06602)

Expression vector of human LIN28.


Drawn by SnapGene® software
Sequence (full) RDB06602hts01.seq
Remarks, protocol and/or map (pdf) RDB06602.pdf
Publication Unpublished bioresource
Test sheet RDB06602C0p1-1.pdf 
Assembled from experimentally sequenced data.
 
Alternative name SET-0226_1
Clone info. Expression vector of human LIN28. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment PCR cloning, forward primer: ggctccgtgtccaaccagcagtt; reverse primer: tcaattctgtgcctccgggagca.
Vector backbone pCMV_S-FLAG (RDB05956) (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human LIN28 cDNA
Depositor|Developer DNA Bank, |

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提供案内 (日本国内) [open/close]

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RDB06602 pCMFlag_hsLIN28 DNA solution

How to cite this biological resource

Materials & Methods section:

The pCMFlag_hsLIN28 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06602).

Reference section:

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

user_report Hayashi, S., Lin28a is a putative factor in regulating cancer stem cell-like properties in side population cells of oral squamous cell carcinoma. Exp Cell Res. 2013 May 1;319(8):1220-8. PMID 23500413. [link to RRC of NBRP]