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pCMFlag_hsLIN28

Expression vector of human LIN28.

Catalog number RDB06602
Resource name pCMFlag_hsLIN28
Alternative name SET-0226_1
Clone info. Expression vector of human LIN28. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment PCR cloning, forward primer: ggctccgtgtccaaccagcagtt; reverse primer: tcaattctgtgcctccgggagca.
Vector backbone pCMV_S-FLAG (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human LIN28 cDNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Human LIN28
          Reference sequence
            
           
          Remarks, protocol and/or map (pdf) RDB06602.pdf

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL in any publication.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 発表に際し、理研BRCから入手したことを明記すること。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB06602 pCMFlag_hsLIN28 Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The pCMFlag_hsLIN28 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06602).

          Reference section:

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          Featured content pCMV_tag and pRSV_tag assay-ready clones (English text)
          Featured content Dnaconda's recommendation EXR0039e (English text)
          Featured content Dnaconda's recommendation EXR0039j (Japanese text)

          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          user_report Hayashi, S., Lin28a is a putative factor in regulating cancer stem cell-like properties in side population cells of oral squamous cell carcinoma. Exp Cell Res. 2013 May 1;319(8):1220-8. PMID 23500413.

          2023.05.22

          GNP_filter3_RDBDEP_html_230512.pl