Expression vector of human LIN28
|Clone info.||Expression vector of human LIN28. Expression was confirmed by Western blotting with anti-FLAG antibody.
F-primer: ggctccgtgtccaaccagcagtt; R-primer: tcaattctgtgcctccgggagca iPS Yamanaka factor.
|Vector backbone||pCMV_S-FLAG (Plasmid)|
|Size of vector backbone||5.5 kb|
|Selectable markers||Amp^r (E. coli), Neo^r (mammalian cell)|
|Gene/insert name||Human LIN28 cDNA|
|Depositor||DNA Bank, ||
|Other clones in our bank||human LIN28 (NCBI Gene 79727) ||
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
|Terms and conditions set forth by the DEPOSITOR||The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL in any publication.|
|Ordering||Please visit Information of Request for Distribution. |
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|Catalog #||Resource name||Shipping form||Fee (non-profit org.)|
|Electronic file||PDF RDB06602.pdf from Depositor|
|Featured content||pCMV_tag and pRSV_tag assay-ready clones (English text)|
|Featured content||Dnaconda's recommendation EXR0039e (English text)|
|Featured content||Dnaconda's recommendation EXR0039j (Japanese text)|
User reports and related articles (go to bottom)
This clone will be sequenced a portion and digested by restriction enzyme for examination.
|user report||Hayashi, S., Lin28a is a putative factor in regulating cancer stem cell-like properties in side population cells of oral squamous cell carcinoma. Exp Cell Res. 2013 May 1;319(8):1220-8. PMID 23500413.|