Expression vector of human LIN28.
|Clone info.||Expression vector of human LIN28. Expression was confirmed by Western blotting with anti-FLAG antibody.
PCR cloning, forward primer: ggctccgtgtccaaccagcagtt; reverse primer: tcaattctgtgcctccgggagca.
|Vector backbone||pCMV_S-FLAG (Plasmid)|
|Size of vector backbone||5.5 kb|
|Selectable markers||Amp^r (E. coli), Neo^r (mammalian cell)|
|Gene/insert name||Human LIN28 cDNA|
|Depositor|Developer||DNA Bank, ||
|Other clones in our bank||human LIN28 (NCBI Gene 79727) ||
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
|Terms and conditions set forth by the DEPOSITOR||The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL in any publication.|
|Ordering||Please visit Information of Request for Distribution.[link] |
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|Catalog #||Resource name||Availability||Shipping form||Fee (non-profit org.)|
|RDB06602||pCMFlag_hsLIN28||Under QC test. Please contact us.||DNA solution|
|Electronic file||Remarks, protocol and/or map (pdf) RDB06602.pdf|
Further references such as user reports and related articles (go to bottom)
|Featured content||pCMV_tag and pRSV_tag assay-ready clones (English text)|
|Featured content||Dnaconda's recommendation EXR0039e (English text)|
|Featured content||Dnaconda's recommendation EXR0039j (Japanese text)|
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
|user report||Hayashi, S., Lin28a is a putative factor in regulating cancer stem cell-like properties in side population cells of oral squamous cell carcinoma. Exp Cell Res. 2013 May 1;319(8):1220-8. PMID 23500413.|