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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCMFlag_hsSox2 (#RDB06600)

Expression vector of human SOX2.

Alternative name SET-0216_5-1
Clone info. Expression vector of human SOX2. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment Derived from IRAUp969A0546D6. PCR cloning, forward primer: atgtacaacatgatggagacgga; reverse primer: tcacatgtgtgagaggggcagt.
Vector backbone pCMV_S-FLAG (RDB05956) (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human Sox2 cDNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB06600.pdf

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RDB06600 pCMFlag_hsSox2 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMFlag_hsSox2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06600).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13105_A5B2p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Sequence file: RDB13105_A5B2a.seq check
>06600_13105_A5B2_1_CMV-Forward_C03_09.ab1
    1 NNNNNNNNNN NNNNTGNNNN NNNGGGCGGT AGGCGTGTAC NGNGGGAGGT CTATATAAGC
   61 AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT
  121 CACTATAGGG AGACCCAAGC TGAATTCACC ATGGACTACA AGGACGATGA CGATAAGGCG
  181 GCCATGTACA ACATGATGGA GACGGAGCTG AAGCCGCCGG GCCCGCAGCA AACTTCGGGG
  241 GGCGGCGGCG GCAACTCCAC CGCGGCGGCG GCCGGCGGCA ACCAGAAAAA CAGCCCGGAC
  301 CGCGTCAAGC GGCCCATGAA TGCCTTCATG GTGTGGTCCC GCGGGCAGCG GCGCAAGATG
  361 GCCCAGGAGA ACCCCAAGAT GCACAACTCG GAGATCAGCA AGCGCCTGGG CGCCGAGTGG
  421 AAACTTTTGT CGGAGACGGA GAAGCGGCCG TTCATCGACG AGGCTAAGCG GCTGCGAGCG
  481 CTGCACATGA AGGAGCACCC GGATTATAAA TACCGGCCCC GGCGGAAAAC CAAGACGCTC
  541 ATGAAGAAGG ATAAGTACAC GCTGCCCGGC GGGCTGCTGG CCCCCGGCGG CAATAGCATG
  601 GCGAGCGGGG TCGGGGTGGG CGCCGGCCTG GGCGCGGGCG TGAACCAGCG CATGGACAGT
  661 TACGCGCACA TGAACGGCTG GAGCAACGGC AGCTACAGCA TGATGCAGGA CCAGCTGGGC
  721 TACCCGCAGC ACCCGGGCCT CAATGCGCAC GGCGCAGCGC AGATGCAGCC CATGCACCGC
  781 TACGACGTGA GCGCCCTGCA GTACAACTCC NTGACCAGCT CGCAGACCTA CATGNACGGC
  841 TCGCCCACCT ACAGCATGTC CTACTCGCAG CANGGCACCC CNNGNATGGN TCTTGNNNNC
  901 NTNNNNNCNG NNGNCAAGTC CGANGCCAGC TNCNGCCCCC CNNGNGGNTA CCNCTTCCTC
  961 CNNCTCCANG GCGCCCNNNC NNNNNNGGGN NNNNNCNGGN NNTNNNCANC NTGNNTNTCC
1021 NNNNNNNNNA NNNNNCNNNN NNNNNN
//
Primer: bGH_rev3
Sequence file: RDB13105_A5B2b.seq check
>06600_13105_B2EqF_1_bGH_rev3_B08_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNGNN GNNGGGNANC NANAGATGGC TGGCAACTAG AAGGCACAGT
   61 CGAGGCTGAT CAGCGAGCTC TAGCATTTAG GTGACACTAT AGAATAGGGC CCTCTAGATG
  121 CATGCTCGAG CGGCCTCACA TGTGTGAGAG GGGCAGTGTG CCGTTAATGG CCGTGCCGGG
  181 CACCGGGCCG CTCTGGTAGT GCTGGGACAT GTGAAGTCTG CTGGGGGCGG CGGGTTCCGG
  241 CACCTCGGCG CCGGGGAGAT ACATGCTGAT CATGTCCCGG AGGTCCCCGG CCTGGCAGGG
  301 CGCCCTGGAG TGGGAGGAAG AGGTAACCAC AGGGGGGCTG GAGCTGGCCT CGGACTTGAC
  361 CACCGAACCC ATGGAGCCAA GAGCCATGCC AGGGGTGCCC TGCTGCGAGT AGGACATGCT
  421 GTAGGTGGGC GAGCCGTTCA TGTAGGTCTG CGAGCTGGTC ATGGAGTTGT ACTGCAGGGC
  481 GCTCACGTCG TAGCGGTGCA TGGGCTGCAT CTGCGCTGCG CCGTGCGCAT TGAGGCCCGG
  541 GTGCTGCGGG TAGCCCAGCT GGTCCTGCAT CATGCTGTAG CTGCCGTTGC TCCAGCCGTT
  601 CATGTGCGCG TAACTGTCCA TGCGCTGGTT CACGCCCGCG CCCAGGCCGG CGCCCACCCC
  661 GACCCCGCTC GCCATGCTAT TGCCGCCGGG GGCCAGCAGC CCGCCGGGCA GCGTGTACTT
  721 ATCCTTCTTC ATGAGCGTCT TGGTTTTCCG CCGGGGCCGG TATTTATAAT CCGGGTGCTC
  781 CTTCATGTGC AGCGCTCGCA GCCGCTTANC CTCGTCGATG ANCGGCCGCT TCTCCGTCTC
  841 CGANNAAAGT TTCCACTCGG CGCCCAGGCG CTTGCTGATC TCCGAGTTGT GCATCTTNGG
  901 GTNCTCCTGG GNCATCTNGC GCCGCTGCCC GCNGGANCAC NNCNTGNANN NNANTNNNGG
  961 GCCGCTNGNN GCNGNCCGGG NTGNTTTTCN NGNTNNNCNG NCNNNNNGNN NNCNNNNNNN
1021 GNNNNNNNNN N
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro,insert 5'
Sequence file: RDB13105_A5B2c.seq check
>06600_13105_A5B2_1_SV40pro_F_V2_E03_15.ab1
    1 NNNNNNNNNN NNNNCNNNGC TTTTGCAAAA GCTCCCGGGN NCNNGGANAT CCNTTTTCGG
   61 ATCTGATCAA GANACNNGAT GAGGNTCGTT TCGCATGATT GAACAAGATG GATTGCACGC
  121 AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT
  181 CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT
  241 CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG
  301 GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG
  361 GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC
  421 TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC
  481 TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA
  541 AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA
  601 ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG
  661 CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG
  721 TGGCCGGCTG GGTGTGGCGG ANCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC
  781 TGAAGAGCTT GGCGGCGAAT GGGCTGANCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC
  841 CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGANGAN TTCTTCTGAN CGGGANTCTN
  901 GGGTTCGAAA TGANCNANNN AGCGACGCCN NACCTGNCNT CNCGAGANTN CNANTNCNNN
961 GNNNNNNTCN ATNNANNNNN GGNNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles