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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCMFlag_hsSox2

Expression vector of human SOX2.

Catalog number RDB06600
Resource name pCMFlag_hsSox2
Alternative name SET-0216_5-1
Clone info. Expression vector of human SOX2. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment Derived from IRAUp969A0546D6. PCR cloning, forward primer: atgtacaacatgatggagacgga; reverse primer: tcacatgtgtgagaggggcagt.
Vector backbone pCMV_S-FLAG (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human Sox2 cDNA
Depositor|Developer DNA Bank, |
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External Database
Human Sox2

          Reference sequence
            

          Distribution information

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          RDB06600 pCMFlag_hsSox2 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB06600.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content pCMV_tag and pRSV_tag assay-ready clones (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13105_A5B2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB13105_A5B2a.seq check
          >06600_13105_A5B2_1_CMV-Forward_C03_09.ab1
              1 CGTCCTCCGC CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
             61 AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT
            121 CACTATAGGG AGACCCAAGC TGAATTCACC ATGGACTACA AGGACGATGA CGATAAGGCG
            181 GCCATGTACA ACATGATGGA GACGGAGCTG AAGCCGCCGG GCCCGCAGCA AACTTCGGGG
            241 GGCGGCGGCG GCAACTCCAC CGCGGCGGCG GCCGGCGGCA ACCAGAAAAA CAGCCCGGAC
            301 CGCGTCAAGC GGCCCATGAA TGCCTTCATG GTGTGGTCCC GCGGGCAGCG GCGCAAGATG
            361 GCCCAGGAGA ACCCCAAGAT GCACAACTCG GAGATCAGCA AGCGCCTGGG CGCCGAGTGG
            421 AAACTTTTGT CGGAGACGGA GAAGCGGCCG TTCATCGACG AGGCTAAGCG GCTGCGAGCG
            481 CTGCACATGA AGGAGCACCC GGATTATAAA TACCGGCCCC GGCGGAAAAC CAAGACGCTC
            541 ATGAAGAAGG ATAAGTACAC GCTGCCCGGC GGGCTGCTGG CCCCCGGCGG CAATAGCATG
            601 GCGAGCGGGG TCGGGGTGGG CGCCGGCCTG GGCGCGGGCG TGAACCAGCG CATGGACAGT
            661 TACGCGCACA TGAACGGCTG GAGCAACGGC AGCTACAGCA TGATGCAGGA CCAGCTGGGC
            721 TACCCGCAGC ACCCGGGCCT CAATGCGCAC GGCGCAGCGC AGATGCAGCC CATGCACCGC
            781 TACGACGTGA GCGCCCTGCA GTACAACTCC ATGACCAGCT CGCAGACCTA CATGAACGGC
            841 TCGCCCACCT ACAGCATGTC CTACTCGCAG CAGGGCACCC CTGGCATGGC TCTTGGCTCC
            901 ATGGGTTCGG TGGTCAAGTC CGAGGCCAGC TCCAGCCCCC CCTGTGGTTA CCTCTTCCTC
            961 CCACTCCAGG GCGCCCTGCC AGGCCGGGGG ACCTCCGGGA CATGATCAGC ATGTATCTCC
           1021 CCGGCGCCGA GGTGCCCGGA ACCCGCCGCC CCCAGCAGAC TTTCACATGT CCCAGCACTA
           1081 CCCAGAGCGG GCCCGGTGCC CGGGCACGGA TCATTAACCG CACACTGACC CCTCCTCACC
          1141 ATGTGACGAG CATCGAGTCC ATGTGCCCCA T
          //
          Primer: BGH_rev
          Sequence file: RDB13105_A5B2b.seq check
          >06600_13105_A5B2_1_BGHrev_H01_22.ab1
              1 GTTAACATTA GTGACTATAG AATAGGGCCC TCTAGATGCA TGCTCGAGCG GCCTCACATG
             61 TGTGAGAGGG GCAGTGTGCC GTTAATGGCC GTGCCGGGCA CCGGGCCGCT CTGGTAGTGC
            121 TGGGACATGT GAAGTCTGCT GGGGGCGGCG GGTTCCGGCA CCTCGGCGCC GGGGAGATAC
            181 ATGCTGATCA TGTCCCGGAG GTCCCCGGCC TGGCAGGGCG CCCTGGAGTG GGAGGAAGAG
            241 GTAACCACAG GGGGGCTGGA GCTGGCCTCG GACTTGACCA CCGAACCCAT GGAGCCAAGA
            301 GCCATGCCAG GGGTGCCCTG CTGCGAGTAG GACATGCTGT AGGTGGGCGA GCCGTTCATG
            361 TAGGTCTGCG AGCTGGTCAT GGAGTTGTAC TGCAGGGCGC TCACGTCGTA GCGGTGCATG
            421 GGCTGCATCT GCGCTGCGCC GTGCGCATTG AGGCCCGGGT GCTGCGGGTA GCCCAGCTGG
            481 TCCTGCATCA TGCTGTAGCT GCCGTTGCTC CAGCCGTTCA TGTGCGCGTA ACTGTCCATG
            541 CGCTGGTTCA CGCCCGCGCC CAGGCCGGCG CCCACCCCGA CCCCGCTCGC CATGCTATTG
            601 CCGCCGGGGG CCAGCAGCCC GCCGGGCAGC GTGTACTTAT CCTTCTTCAT GAGCGTCTTG
            661 GTTTTCCGCC GGGGCCGGTA TTTATAATCC GGGTGCTCCT TCATGTGCAG CGCTCGCAGC
            721 CGCTTAGCCT CGTCGATGAA CGGCCGCTTC TCCGTCTCCG ACAAAAGTTT CCACTCGGCG
            781 CCCAGGCGCT TGCTGATCTC CGAGTTGTGC ATCTTGGGGT TCTCCTGGGC CATCTTGCGC
            841 CGCTGCCCGC GGGACCACAC CATGAAGGCA TTCATGGGCC GCTTGACGCG GTCCGGGCTG
            901 TTTTTCTGGT TGCCGCCGGC CGCCGCCGCG GTGGAGTTGC CGCCGCCGCC CCCCCGAAGT
            961 TTGCTGCGGG CCCGGCGGCT TCAGCTCCGT CTCCATCATG TTGTACATGG GCCGCCCTTA
           1021 TCGTCATCGT CCTTTGTAGT CCCATGGTGA ATTTCAGCTT TGGGTCTCCC TATAGTGAGT
           1081 CGTATTAAAT TTCCGATAGT AAGTTAGCCA GTTGCGACTC TAGCTTAGCC CAGAAGGAAG
          1141 CTCTCTGTGC C
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl