Please review the QC test results indicated by icon below as well as clone information before placing your order.

pGEM3Zf_hsKlf4(F) (#RDB06431)

Plasmid clone of human Klf4 cDNA.

Alternative name	SET-0217_1
Clone info.	Plasmid clone of human Klf4 cDNA.
Comment	This clone contains human KLF4 similar to Dr. Yamanaka's construct in the Cell (2007), 131 (5), 861-872, PMID 18035408 (333 to 1742 of NM_004235.3, cds 330 to 1742). However, this fragment corresponds 579 to 1988 of recent version of the RefSeq ( NM_004235.5, cds 549 to 1988). Derived from IRATp970H0848D6. PCR cloning, forward primer: gctgtcagcgacgcgctgctccca; reverse primer: ttaaaaatgcctcttcatgtgtaaggc.
Vector backbone	pGEM-3Zf(+) (Plasmid)
Size of vector backbone	3,197 bp
Selectable markers	Amp^r
Gene/insert name	Human Klf4 cDNA
Depositor|Developer	DNA Bank, |

 

Distribution information

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提供案内 (日本国内) [open/close]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB06431	pGEM3Zf_hsKlf4(F)	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The pGEM3Zf_hsKlf4(F) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06431).

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13524_A5Ipp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 Sequence file: RDB13524_A5Ipa.seq
>06431_13524_A5Ip_M13(-40)_F02_17.ab1     1 CGAGCCCGTT GGATTTGTAA TACGACTCAC TATAGGGCGA ATTCGAGCTC GGTACCCGGG    61 GATCCTCTAG AGTCTTAAAA ATGCCTCTTC ATGTGTAAGG CGAGGTGGTC CGACCTGGAA   121 AATGCTCGGT CGCATTTTTG GCACTGGAAC GGGCGGTGCC CCGTGTGTTT ACGGTAGTGC   181 CTGGTCAGTT CATCTGAGCG GGCGAATTTC CATCCACAGC CGTCCCAGTC ACAGTGGTAA   241 GGTTTCTCAC CTGTGTGGGT TCGCAGGTGT GCCTTGAGAT GGGAACTCTT TGTGTAGGTT   301 TTGCCGCAGC CCGCGTAATC ACAAGTGTGG GTGGCGGTCC TTTTCCGGGG CCACGATCGT   361 CTTCCCCTCT TTGGCTTGGG CTCCTCTGGC ATGCAGGAAC CGGGTGGCAT GAGCTCTTGG   421 TAATGGAGCG GCGGGACTTG CGGCTGCATC TGATCGGGCA GGAAGGATGG GTAATTGGGC   481 CCCGGGTGGG GATGGAAGCC GGGAGGAAGC GGCAGGGCAG GGTGACAGTC CCTGCTGCTC   541 AGCACTTCCT CAAGACCCAG GGTCGGGGTA GTCCTGCTGG GGAGCTGCCG CCCCAGGGGG   601 AAGTCGTGTG CAGCCGGCCG GTGGCCATTG CTGAGAGGGG GTCCAGCGCC CAAGTGGGTG   661 CACGAAGAGA CCGCCTCCTG CTTGATCTTG GGGCACGTGC GCGGCGGGCC CGCCGTTGTA   721 GGGCGCCACC ACCACCGGGT GGCTGCCGTC AGGGCTGCCT TTGCTGACGC TGATGACCCG   781 ACGGGCTGCC GGTACTCGCT GCCCAGGGGC GCTCAGCGAC GCCTTCAGCA CGAACTTGCC   841 CATCAGCCCG CCACCTGGCG GCTGCGGCTG CTGCGGCGGA ATTGTAACAC CGGGTCCAAT   901 TCTGGGCCCG CAGGAGCTCG GCCCACGAAG CCGCCCGAGG GGCTCACGTC GTTGATGTCC   961 GCCAAGGTTT GAAGGGAGCC GTCGGAGGGG GGGAGCGGGA CTTCCCTGCC ATAAAGGAGG  1021 CCTCCGCCCG TTGCCGCCCC GGGCGCCACG CCCCGGGGTC GGTTCCCGGG CCCGGGAATC 1081 GGATAGGGTG AAACCTGCCA GGTGGAAAGG GC //
Primer: Reverse2 Sequence file: RDB13524_A5Ipb.seq
>06431_13524_A5Ip_Reverse2_G02_20.ab1     1 AGGAATTATT ACGCCAGCTA TTTAGGTGAC ACTATAGAAT ACTCAAGCTT GCATGCCTGC    61 AGGTCGCTGT CAGCGACGCG CTGCTCCCAT CTTTCTCCAC GTTCGCGTCT GGCCCGGCGG   121 GAAGGGAGAA GACACTGCGT CAAGCAGGTG CCCCGAATAA CCGCTGGCGG GAGGAGCTCT   181 CCCACATGAA GCGACTTCCC CCAGTGCTTC CCGGCCGCCC CTATGACCTG GCGGCGGCGA   241 CCGTGGCCAC AGACCTGGAG AGCGGCGGAG CCGGTGCGGC TTGCGGCGGT AGCAACCTGG   301 CGCCCCTACC TCGGAGAGAG ACCGAGGAGT TCAACGATCT CCTGGACCTG GACTTTATTC   361 TCTCCAATTC GCTGACCCAT CCTCCGGAGT CAGTGGCCGC CACCGTGTCC TCGTCAGCGT   421 CAGCCTCCTC TTCGTCGTCG CCGTCGAGCA GCGGCCCTGC CAGCGCGCCC TCCACCTGCA   481 GCTTCACCTA TCCGATCCGG GCCGGGAACG ACCCGGGCGT GGCGCCGGGC GGCACGGGCG   541 GAGGCCTCCT CTATGGCAGG GAGTCCGCTC CCCCTCCGAC GGCTCCCTTC AACCTGGCGG   601 ACATCAACGA CGTGAGCCCC TCGGGCGGCT TCGTGGCCGA GCTCCTGCGG CCAGAATTGG   661 ACCCGGTGTA CATTCCGCCG CAGCAGCCGC AGCCGCCAGG TGGCGGGCTG ATGGGCAAGT   721 TCGTGCTGAA GGCGTCGCTG AGCGCCCCTG GCAGCGAGTA CGGCAGCCCG TCGGTCATCA   781 GCGTCAGCAA AGGCAGCCCT GACGGCAGCC ACCCGGTGGT GGTGGCGCCC TACAACGGCG   841 GGCCGCCGCG CACGTGCCCC AAGATCAAGC AGGAGGCGGT CTCTTCGTGC ACCCACTTGG   901 GCGCTGGACC CCCTCTCAGC AATGGCCACC GGCCGGCTGC ACACGACTTC CCCCTGGGGC   961 GGCAGCTCCC CAGCAGGACT ACCCCGACCC TGGGTCTTGA GGAAGTGCTG AGCAGCAGGA  1021 CTGTCACCCC TGCCCCTGCC GCTTTCTCCG GCTTCCATCC CCCACCCGGG GGCCATACCA  1081 TCGTCTGCCC GATCCGATGC AGCGGCAAGG TCCCGCCGCT CATTACTAGA GCTCAATGCA 1141 TCGGATCATG CATGCCAGAA AGGG //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

Resource search

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Link

Other resources in our bank

• human Klf4

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External Database
Human Klf4

• in KEGG Gene
• in KEGG Orthology
• in NCBI Gene

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Reference sequence
  

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