Please review the QC test results indicated by 
icon below as well as clone information before placing your order.
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Please review the QC test results indicated by icon below as well as clone information before placing your order.
icon below as well as clone information before placing your order.
pCMFlag_hsMAFG (#RDB06413)
Expression vector of human MAFG.
| Alternative name | SET-0065_6 |
|---|---|
| Clone info. | Expression vector of human MAFG. Expression was confirmed by Western blotting with anti-FLAG antibody. |
| Comment | Derived from Sugano BC012327. PCR cloning, forward primer: ATGACGACCCCCAATAAAGG; reverse primer: CTACGATCGGGCATCCGTCT. |
| Vector backbone | pCMV_S-FLAG (RDB05956) (Plasmid) |
| Size of vector backbone | 5.5 kb |
| Selectable markers | Amp^r (E. coli), Neo^r (mammalian cell) |
| Gene/insert name | Human MAFG cDNA |
| Depositor|Developer | DNA Bank, | |
| Remarks, protocol and/or map (pdf) | RDB06413.pdf |
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Distribution information
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Ordering forms | Order form [Credit Card Payment  ] [Bank Transfer Payment] [Example of order form ]MTA, for use for not-for-profit academic purpose [Word ] [Example of MTA ]Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
|---|---|
| Terms and conditions for distribution | The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL and cite Gene 200, 149-156, 1997 in any publication. |
[open/close]| 必要書類 | 提供依頼書 [依頼書の記入例 ]提供同意書 (MTA, 非営利学術目的用)[Word ] [MTAの記入例 ]手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
|---|---|
| MTAに書く使用条件 | 発表に際し、理研BRCから入手したことを明記し、指定する文献(Gene, 200, 149-156 (1997))を引用すること。 |
| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB06413 | pCMFlag_hsMAFG | DNA solution |
icon below as well as clone information before placing your order.How to cite this biological resource
Materials & Methods section:
| The pCMFlag_hsMAFG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06413). |
Reference section:
Further references such as user reports and related articles (go to bottom)
QC test results
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB13708_A5Kap1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: CMV-Forward Sequence file: RDB13708_A5Kaa.seq
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>06413_13708_A5Ka_CMV-Forward_A05_02.ab1 1 ATTAACTCGC CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC 61 AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT 121 CACTATAGGG AGACCCAAGC TGAATTCACC ATGGACTACA AGGACGATGA CGATAAGGCG 181 GCCATGACGA CCCCCAATAA AGGAAACAAG GCCTTGAAGG TGAAGCGGGA GCCGGGTGAG 241 AATGGCACCA GCCTGACGGA TGAGGAGCTG GTGACCATGT CGGTGCGGGA GCTGAACCAG 301 CACCTGCGGG GCCTGTCCAA GGAGGAGATC GTCCAGCTGA AGCAGCGCCG GCGCACGCTC 361 AAGAACCGCG GCTACGCTGC CAGCTGCCGC GTGAAGCGGG TGACGCAGAA GGAGGAGCTG 421 GAGAAGCAGA AGGCGGAGCT GCAGCAGGAG GTGGAGAAGC TGGCCTCAGA GAACGCCAGC 481 ATGAAGCTGG AGCTCGACGC GCTGCGCTCC AAGTACGAGG CGCTGCAGAC CTTCGCCCGG 541 ACGGTGGCCC GCAGCCCCGT GGCGCCAGCC CGGGGCCCCC TTGCCGCCGG CCTGGGGCCC 601 CTCGTCCCAG GCAAGGTGGC CGCCACCAGC GTCATCACAA TAGTAAAGTC CAAGACGGAT 661 GCCCGATCGT AGGGCCGCTC GAGCATGCAT CTAGAGGGCC CTATTCTATA GTGTCACCTA 721 AATGCTAGAG CTCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT 781 TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA 841 TAAAATGAGG AAATTGCATC GCATTGTCTG AGTATGTGTC ATTCTATTCT GGGGTGTGGT 901 TTAGCCAGGA CAGCAAGGGG GGGGAGGGAT TGGGAAGACC ATAGCAGGGC ATGCTGGGGA 961 ATGCGTGGGC TCTATGGCTT CCTGAGGCGG AAGAACCTAG CTGGAGCTCG AAGGGGAATC 1021 GCCACGTCGC CTGTAGCGGC CGCATT // |
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Primer: SV40pro_F_V2 Sequence file: RDB13708_A5Kab.seq
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>06413_13708_A5Ka_SV40pro_F_V2_B05_05.ab1 1 GGGGACGTTT TGGAGCTAGG CTTTTGCAAA AGCTCCCGGG AGCTTGGATA TCCATTTTCG 61 GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG 121 CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA 181 TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG 241 TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT 301 GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA 361 GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC 421 CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG 481 CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG 541 AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG 601 AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG 661 GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT 721 GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG 781 CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC 841 CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT 901 GGGGGTTCGA AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA 961 CCGCCGCCCT TCTATGAAGG GTTTGGGGCT TCGGAATCGT TTTCCCGGGG ACGCCCGGCT 1021 GGATTGATTC CTTCCAGCGG CGGGGATCTC AATGCTGGGA AGTCCATCGG CCCAACCCCA 1081 ACTTGTTTTA TTTGCCAGGC GCCAG // |
Please visit 
Sequencing and PCR primers for primer information.
References
Original, user report and related articles
Resource search
Link
Other resources in our bankExternal Database
Human MAFG
Reference sequence
Tips
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