Resource data sheet
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pRL-CMV mut1

Expression vector of Renilla luciferase, mutated CMV promoter

Catalog number RDB06408
Resource name pRL-CMV mut1
Clone info. Expression vector of Renilla luciferase, mutated CMV promoter. AP-1 sequence (TGACTCA) appeared in the CMV promoter was substituted with TGCCTCG.
Derived from pRL-CMV.
Vector backbone pRL-CMV (Plasmid)
Size of vector backbone 4,079 bp
Selectable markers Amp^r
Gene/insert name Renilla reniformis luciferase cDNA
Depositor Yokoyama, Kazunari |

Distribution information

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Terms and conditions set forth by the DEPOSITOR In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB06408 pRL-CMV mut1 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Map RDB06408z.gif from Depositor

Featured content

Featured content Control Vectors (English text)

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15082_A7Etp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB15082_A7Eta.seq
>06408_15082_A7Et_1_phRLR2_B06_06_ABI24.ab1
    1 ATTTCTCTGT CTCGACAGCC CAGTTTCTAT TGGTCTCCTT AAACCTGTCT TGTAACCTTG
   61 ATACTTACCT GCCCAGTGCC TCACGACCAA CTTCTGCAGC TTAAGTTCGA GACTGTTGTG
  121 TCAGAAGCAC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCAAT AAAGCTTCTA
  181 GTGATCTGAC GGTTCACTAA ACGAGCTCTG CTTATATAGA CCTCCCACCG TACACGCCTA
  241 CCGCCCATTT GCGTCAACGG GGCGGGGTTA TTACGACATT TTGGAAAGTC CCGTTGATTT
  301 TGGTGCCAAA ACAAACTCCC ATTGACGTCA ATGGGGTGGA GACTTGGAAA TCCCCGCGAG
  361 GCAAACCGCT ATCCACGCCC ATTGGTGTAC TGCCAAAACC GCATCACCAT GGTAATAGCG
  421 ATGACTAATA CGTAGATGTA CTGCCAAGTA GGAAAGTCCC GTAAGGTCAT GTACTGGGCA
  481 TAATGCCAGG CGGGCCATTT ACCGTCATTG ACGTCAATAG GGGGCGGACT TGGCATATGA
  541 TACACTTGAT GTACTGCCAA GTGGGCAGTT TACCGTAAAT ACTCCACCCA TTGACGTCAA
  601 TGGAAAGTCC CTATTGGCGT TACTATGGGA ACATACGTCA TTATTGACGT CAATGGGCGG
  661 GGGTCGTTGG GCGGTCAGCC AGGCGGGCCA TTTACCGTAA GTTATGTAAC GCGGAACTCC
  721 ATATATGGGC TATGAACTAA TGACCCCGTA ATTGATTACT ATTAATAACT AGTCAATAAT
  781 CAATGCCAAC ATGGCGGTCA TATTGGACAT GAGCCAATAT AAATGTACAT ATTATGATAT
  841 AGATACAACG TATGCAATGG CCAATAGCCA ATATTGATTT ATGCTATATA ACCAATGAAT
  901 AATATGGCTA ATGGCCAATA TTGAAGATCT GTCGAGCCAT GTGAGCAAAA GGCCAGCAAA
  961 AGGCCAGGAA CCCGTAAAAA GGCCGCGTTG CTGGCGTTTT TTCCATAGGC TCCGCCCCCC
 1021 CTGACGAGCA TCACAAAAAA ATCGACGCTC AAGTCAGAAG TGGCGAAAAC CCGACCAGAC
 1081 TATAAAGAAT ACCCAGTCGA TCCCCATGGA AGCTCCCTCG TGCGCCTCCT CCTGGATTCC
1141 GGAACCCCAT TGTCG
//
Primer: phRLR2 RDB15082_A7Etb.seq
>06408_15082_A7Et_1_CMV-Forward_A06_03_ABI24.ab1
    1 TGATATACCC CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TCGTTTAGTG AACCGTCAGA TCACTAGAAG CTTTATTGCG GTAGTTTATC
  121 ACAGTTAAAT TGCTAACGCA GTCAGTGCTT CTGACACAAC AGTCTCGAAC TTAAGCTGCA
  181 GAAGTTGGTC GTGAGGCACT GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG
  241 ACCAATAGAA ACTGGGCTTG TCGAGACAGA GAAGACTCTT GCGTTTCTGA TAGGCACCTA
  301 TTGGTCTTAC TGACATCCAC TTTGCCTTTC TCTCCACAGG TGTCCACTCC CAGTTCAATT
  361 ACAGCTCTTA AGGCTAGAGT ACTTAATACG ACTCACTATA GGCTAGCCAC CATGACTTCG
  421 AAAGTTTATG ATCCAGAACA AAGGAAACGG ATGATAACTG GTCCGCAGTG GTGGGCCAGA
  481 TGTAAACAAA TGAATGTTCT TGATTCATTT ATTAATTATT ATGATTCAGA AAAACATGCA
  541 GAAAATGCTG TTATTTTTTT ACATGGTAAC GCGGCCTCTT CTTATTTATG GCGACATGTT
  601 GTGCCACATA TTGAGCCAGT AGCGCGGTGT ATTATACCAG ACCTTATTGG TATGGGCAAA
  661 TCAGGCAAAT CTGGTAATGG TTCTTATAGG TTACTTGATC ATTACAAATA TCTTACTGCA
  721 TGGTTTGAAC TTCTTAATTT ACCAAAGAAG ATCATTTTTG TCGGCCATGA TTGGGGTGCT
  781 TGTTTGGCAT TTCATTATAG CTATGAGCAT CAAGATAAGA TCAAAGCAAT AGTTCACGCT
  841 GAAAGTGTAG TAGATGTGAT TGAATCATGG GATGAATGGC CTGATATTGA AGAAGATATT
  901 GCGTTGATCA AATCTGAAGA AGGAGAAAAA AATGGTTTTG GAGAATAACT TCTTTCGTGG
  961 AAAACCATGT TTGCCATCAA AAATCATGAG AAAAGTTAGA ACCAGAAGAA TTTTGCAGCA
 1021 TATCTTGAAC CCATTTCAAG AGAAAGTGAG TTCGTCGTCC ACATTATCAT TGCTCGTGAA
 1081 TCCCGTTAGT AAGTCTAACT TGAACGTGGT ACAATTGTTA AGAACTTTAT TAAAGTGGCC
1141 C
//
Primer: pAxCALNL_F1 RDB15082_A7Etc.seq
>06408_15082_A7Et_1_pAxCALNL_F1_C06_09_ABI24.ab1
    1 CCTATGTATC TTATCATGTC TGCTCGAAGC GGCCGCTCTA GAATTATTGT TCATTTTTGA
   61 GAACTCGCTC AACGAACGAT TTGATATATT TTCCCATTTC ATCAGGTGCA TCTTCTTGCG
  121 AAAAATGAAG ACCTTTTACT TTGACAAATT CAGTATTAGG AAACTTCTTG GCACCTTCAA
  181 CAATAGCATT GGAAAAGAAT CCTGGGTCCG ATTCAATAAA CATTTTTGGT AAATCATCAC
  241 TTGCACGTAG ATAAGCATTA TAATTCCTAA CAATTTGTAC AACGTCAGGT TTACCACCTT
  301 TTACTAACGG GATTTCACGA GGCCATGATA ATGTTGGACG ACGAACTTCA CCTTTCTCTT
  361 TGAATGGTTC AAGATATGCT GCAAATTCTT CTGGTTCTAA CTTTCTCATG ATTTTTGATG
  421 GCAACATGGT TTCCACGAAG AAGTTATTCT CCAAAACCAT TTTTTCTCCT TCTTCAGATT
  481 TGATCAACGC AATATCTTCT TCAATATCAG GCCATTCATC CCATGATTCA ATCACATCTA
  541 CTACACTTTC AGCGTGAACT ATTGCTTTGA TCTTATCTTG ATGCTCATAG CTATAATGAA
  601 ATGCCAAACA AGCACCCCAA TCATGGCCGA CAAAAATGAT CTTCTTTGGT AAATTAAGAA
  661 GTTCAAACCA TGCAGTAAGA TATTTGTAAT GATCAAGTAA CCTATAAGAA CCATTACCAG
  721 ATTTGCCTGA TTTGCCCATA CCAATAAGGT CTGGTATAAT ACACCGCGCT ACTGGCTCAA
  781 TATGTGGCAC AACATGTCGC CATAAATAAG AAGAGGCCGC GTTACCATGT AAAAAAAATA
  841 ACAGCATTTT CTGCATGTTT TTCTGAATCA TAATAATTAA TAAATGAATC AAGAACATTC
  901 ATTTGTTTAC ATCTGGCCCA CCACTGCGGA CCAGTTATCA TCCGTTTCCT TTGTTCTGGA
  961 TCATAAACTT TCGAAGTCAT GGGTGGCTAG CCCTATAGTG AGTCGTATTA AGTACTCTAG
 1021 CCCTTAAGAG CTGTAATTTG AACTGGGAGT GACACTGGTG GAGAGAACGC AAAGTGGAAT
 1081 GTCAGTAGAC TATTAGTGCT ATCGAACGCA GAGTCTTCTC TGGTCTCGAA CAAAGCTCAG
1141 GTTCCGAAGT G
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.12.10

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