Resource data sheet
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CSII-EF-mRFP1

Expression vector of RFP driven by EF-1 alpha promoter

Catalog number RDB06387
Resource name CSII-EF-mRFP1
Clone info. Expression vector of RFP driven by EF-1 alpha promoter.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone Plasmid
Selectable markers Amp^r
Gene/insert name Discosoma sp. RFP cDNA
Originator Miyoshi, Hiroyuki |

Distribution information

CLICK here for plasmid list and map. [link]
Forms for plasmid request [in Japanese] [in English]
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
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Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus. Please contact us. We will provide you with appropriate documents.
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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB07977_A3G2p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxEF_F1 RDB07977_A3G2a.seq
>06387_07977_A3G2_pAxEF_F1_E05_14.ab1
    1 ATGTTTTCTC ATTTCAGTGT CGTGACACGC TACCGGTCTC GACTGGATCC GATGGCCTCC
   61 TCCGAGGACG TCATCAAGGA GTTCATGCGC TTCAAGGTGC GCATGGAGGG CTCCGTGAAC
  121 GGCCACGAGT TCGAGATCGA GGGCGAGGGC GAGGGCCGCC CCTACGAGGG CACCCAGACC
  181 GCCAAGCTGA AGGTGACCAA GGGCGGCCCC CTGCCCTTCG CCTGGGACAT CCTGTCCCCT
  241 CAGTTCCAGT ACGGCTCCAA GGCCTACGTG AAGCACCCCG CCGACATCCC CGACTACTTG
  301 AAGCTGTCCT TCCCCGAGGG CTTCAAGTGG GAGCGCGTGA TGAACTTCGA GGACGGCGGC
  361 GTGGTGACCG TGACCCAGGA CTCCTCCCTG CAGGACGGCG AGTTCATCTA CAAGGTGAAG
  421 CTGCGCGGCA CCAACTTCCC CTCCGACGGC CCCGTAATGC AGAAGAAGAC CATGGGCTGG
  481 GAGGCCTCCA CCGAGCGGAT GTACCCCGAG GACGGCGCCC TGAAGGGCGA GATCAAGATG
  541 AGGCTGAAGC TGAAGGACGG CGGCCACTAC GACGCCGAGG TCAAGACCAC CTACATGGCC
  601 AAGAAGCCCG TGCAGCTGCC CGGCGCCTAC AAGACCGACA TCAAGCTGGA CATCACCTCC
  661 CACAACGAGG ACTACACCAT CGTGGAACAG TACGAGCGCG CCGAGGGCCG CCACTCCACC
  721 GGCGCCTAAG AATTCGCGGC CGCGGATCCT CTAGAGTTAA CATCGAGGGA TCAAGCTTAT
  781 CGATAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT
  841 TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC
  901 CCGTATGGCT TTCATTTTTC TCCTCCTTGT ATAAATCCTG GTTGCTGTCT CTTTATGAGG
  961 AGTTGTGGCC CGTTGTCAGG CAACGTGGCG TGGTGTGCAC TGTGTTTGCT GACGCAACCC
 1021 CCCCACTGGG TTTGGGGCAT TTGCCACCAC CTGTCAGCTC GTTTCGGGGA CTTTCGCTTT
1081 TCCCCATCCT TATGGCACGG GCGAACCTCA TCGCCCGTCC TACCTTTGAT G
//
Primer: CMV-Forward RDB07977_A3G2b.seq
>06387_07977_A3G2_CMV-Forward_D05_11.ab1
    1 AGTCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGCGCGTTT TGCCTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT
  121 CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG
  181 TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT
  241 CAGTGTGGAA AATCTCTAGC AGTGGCGCCC GAACAGGGAC TTGAAAGCGA AAGGGAAACC
  301 AGAGGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG
  361 GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG
  421 GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT
  481 AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT
  541 AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT
  601 GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC
  661 AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AAGGAAGCTT
  721 TAGACAAGAT AGAGGAAGAG CAAAACAAAA GTAAGACCAC CGCACAGCAA GGGCCGCTGA
  781 TCTTCAGACC CTGGAGGAGA GATATGAGGG ACATTGGAGA GTGATTATAT AAATAATAAA
  841 GTAGTAAAAA TTGACCATTT AGAGTAGCAA CCCAACAGCA AAGAGAAGGA GTGGTGCAGA
  901 GAGAAAAAGA GCAGTGGGGA ATAGGAGCTT TGGTCTGGAT CTGGGAGCAG CAGAGCACTT
  961 ATGGGCGCAA CGTTCATTGA CGCTGACCGG TTACAGGCCG ACACATATGG CCTGCATATG
1021 TGGCACAGTC GGAACCA
//
Primer: BGH_rev RDB07977_A3G2c.seq
>06387_07977_A3G2_BGH_rev_C05_08.ab1
    1 GCATCGGGGT CTGCTAGAGA TTTTCCACAC TGACTAAAAG GGTCTGAGGG ATCTCTAGTT
   61 ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC ACTCAAGGCA AGCTTTATTG
  121 AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC CAGGCTCAGA TCTGGTCTAA
  181 CCAGAGAGAC CCAGTACAGT CCGGATGCAG CTCTCGGGCC ATGTGATGAA ATGCTAGGCG
  241 GCTGTCAAAC CTCCACTCTA ATACTTCTCT CTCCGGGTCA TCCATCCCAT GCAGGCTCAC
  301 AGGGTGTAAC AAGCGGGTGT TCTCTCCTTC ATTGGCTTCT TCTACCTTCT CTTGCTCAAC
  361 TGGTACTAGC TTGTAGCACC ATCCAAAGGT CAGTGGATAT CTGATCCCTG GCCCTGGTGT
  421 GTAGTTCTGC CAATCAGGGA AGTAGCCTTG TGTGTGGTAG ATCCACAGAT CAAGGATATC
  481 TTGTCTTCGT TGGGAGTGAA TTAGCCCTTC CAGTCCCCCC TTTTCTTTTA AAAAGTGGCT
  541 AAGATCTACA GCTGCCTTGT AAGTCATTGG TCTTAAAGGT ACCTGAGGTG TGACTGGAAA
  601 ACCCACCTCC TCCTCCTCTT GTGCTTCTAG CCAGGCACAA TCAGCATTGG TAGCTGCTGT
  661 ATTGCTACTT GTGATTGCTC CATGTTTTTC TAGGTCTCGA CGGTATCGAT GCGGGGAGGC
  721 GGCCCAAAGG GAGATCCGAC TCGTCTGAGG GCGAACGCGA AGACGCGGAA GAGGCCGCAG
  781 AGTCCGGCAG CAGGCCGCGG GAAGGAAGGT CCGCTGGATT GAGGGCCGAA GGGACGTAGC
  841 AGAAGGACGT CCCGCGCAGA ATCCAGGTGG CACACAGGCG AGCAGCCATG GAAGACGTCA
  901 GCTTTCCCCG ACACACACGG ATGTCAGTGC CCACAGCGAG CCCTGTCAGC AGCGGCAGCA
  961 GCGGCGATGA GTCCGGCTGG CCATAGGAGG GGAGCAAAGT CCGGAGGACT GAACGGTGAT
1021 GCCAATGCCT CACATGGATG CTCGCATCAG TGCACTCAGG CCA
//
Primer: SV40pro_F RDB07977_A3G2d.seq
>06387_07977_A3G2_SV40pro_F_F05_17.ab1
    1 CCCTGTCCTT GCATTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
   61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA GCACGTGTTG ACAATTAATC
  121 ATCGGCATAG TATATCGGCA TAGTATAATA CGACAAGGTG AGGAACTAAA CCATGGCCAA
  181 GTTGACCAGT GCCGTTCCGG TGCTCACCGC GCGCGACGTC GCCGGAGCGG TCGAGTTCTG
  241 GACCGACCGG CTCGGGTTCT CCCGGGACTT CGTGGAGGAC GACTTCGCCG GTGTGGTCCG
  301 GGACGACGTG ACCCTGTTCA TCAGCGCGGT CCAGGACCAG GTGGTGCCGG ACAACACCCT
  361 GGCCTGGGTG TGGGTGCGCG GCCTGGACGA GCTGTACGCC GAGTGGTCGG AGGTCGTGTC
  421 CACGAACTTC CGGGACGCCT CCGGGCCGGC CATGACCGAG ATCGGCGAGC AGCCGTGGGG
  481 GCGGGAGTTC GCCCTGCGCG ACCCGGCCGG CAACTGCGTG CACTTCGTGG CCGAGGAGCA
  541 GGACTGACAC GTGCTACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT
  601 CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA
  661 GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG
  721 CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA
  781 ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA
  841 ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT
  901 ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT
  961 AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CCTGTCGTGC CAGCTGCATT
 1021 AAATGAAATC GGGCCACGCG CGGGGGAGAG GCGGGTTTGC CGTATGGGCG CTCTTCGCTT
 1081 CTCGCTCACT GAACTCGCTT GCGCCTCGGA TTCGGATTCG GGCCGTTGCC GGCCGGAAAT
1141 G
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

References

user report Yoshida, S., Generation of stratified squamous epithelial progenitor cells from mouse induced pluripotent stem cells. PLoS One, 6 (12): e28856 (2012). PMID 22174914.

2017.10.23

GNP_filter3_RDBDEP_171017_html.pl