Resource data sheet
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CSII-CMV-mRFP1

Expression vector of RFP driven by CMV promoter

Catalog number RDB06386
Resource name CSII-CMV-mRFP1
Clone info. Expression vector of RFP driven by CMV promoter.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone Plasmid
Selectable markers Amp^r
Gene/insert name Discosoma sp. RFP cDNA
Originator Miyoshi, Hiroyuki |

Distribution information

CLICK here for plasmid list and map. [link]
Forms for plasmid request [in Japanese] [in English]
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
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Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus. Please contact us. We will provide you with appropriate documents.
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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB07991_A3G2p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: PRE_rev RDB07991_A3G2a_v2.seq
>06386_07991_A3G2_PRE_rev_D07_10.ab1
    1 AGTATCAAAT AGCGTAAAGG AGCAACATAG TTAAGAATAC CAGTCAATCT TTCACAAATT
   61 TTGTAATCCA GAGGTTGATT ATCGATAAGC TTGATCCCTC GATGTTAACT CTAGAGGATC
  121 CGCGGCCGCG AATTCTTAGG CGCCGGTGGA GTGGCGGCCC TCGGCGCGCT CGTACTGTTC
  181 CACGATGGTG TAGTCCTCGT TGTGGGAGGT GATGTCCAGC TTGATGTCGG TCTTGTAGGC
  241 GCCGGGCAGC TGCACGGGCT TCTTGGCCAT GTAGGTGGTC TTGACCTCGG CGTCGTAGTG
  301 GCCGCCGTCC TTCAGCTTCA GCCTCATCTT GATCTCGCCC TTCAGGGCGC CGTCCTCGGG
  361 GTACATCCGC TCGGTGGAGG CCTCCCAGCC CATGGTCTTC TTCTGCATTA CGGGGCCGTC
  421 GGAGGGGAAG TTGGTGCCGC GCAGCTTCAC CTTGTAGATG AACTCGCCGT CCTGCAGGGA
  481 GGAGTCCTGG GTCACGGTCA CCACGCCGCC GTCCTCGAAG TTCATCACGC GCTCCCACTT
  541 GAAGCCCTCG GGGAAGGACA GCTTCAAGTA GTCGGGGATG TCGGCGGGGT GCTTCACGTA
  601 GGCCTTGGAG CCGTACTGGA ACTGAGGGGA CAGGATGTCC CAGGCGAAGG GCAGGGGGCC
  661 GCCCTTGGTC ACCTTCAGCT TGGCGGTCTG GGTGCCCTCG TAGGGGCGGC CCTCGCCCTC
  721 GCCCTCGATC TCGAACTCGT GGCCGTTCAC GGAGCCCTCC ATGCGCACCT TGAAGCGCAT
  781 GAACTCCTTG ATGACGTCCT CGGAGGAGGC CATCGGATCC AGTCGAGACC GGTAGCGCTA
  841 GCGGATCTGA CGGTTCACTA AACCAGCTCT GCTTATATAG ACCTCCCACC GTACACGCCT
  901 ACCGCCCATT TGCGTCAATG GGGGCGGAGT TGTTTACGAC ATTTTTGGAA AGTCCCGTTT
  961 GATTTTTGGG TGCCCAAAAC AAACTCCCAT TTGACGTCAA TGGGGGTGGA GACTTTGGGA
 1021 AATCCCCGTG AGTCAAACCG CTATCCACGC CCAATTGGAT GTACTGGCCT AAACGCATTC
1081 ACCAATGGTA ATAGCCGAAT GCACCTTAAA TAACG
//
Primer: HIVgp_R1 RDB07991_A3G2b_v2.seq
>06386_07991_A3G2_HIVgp_R1_E07_13.ab1
    1 CGGCCATCTA TTCCCACTGC TCTTTTTTCT CTCTGCACCA CTCTTCTCTT TGCCTTGGTG
   61 GGTGCTACTC CTAATGGTTC AATTTTTACT ACTTTATATT TATATAATTC ACTTCTCCAA
  121 TTGTCCCTCA TATCTCCTCC TCCAGGTCTG AAGATCAGCG GCCGGCCGCT TGCTGTGCGG
  181 TGGTCTTACT TTTGTTTTGC TCTTCCTCTA TCTTGTCTAA AGCTTCCTTG GTGTCTTTTA
  241 TCTCTATCCT TTGATGCACA CAATAGAGGG TTGCTACTGT ATTATATAAT GATCTAAGTT
  301 CTTCTGATCC TGTCTGAAGG GATGGTTGTA GCTGTCCCAG TATTTGTCTA CAGCCTTCTG
  361 ATGTTTCTAA CAGGCCAGGA TTAACTGCGA ATCGTTCTAG CTCCCTGCTT GCCCATACTA
  421 TATGTTTTAA TTTATATTTT TTCTTTCCCC CTGGCCTTAA CCGAATTTTT TCCCATCGCG
  481 ATCTAATTCT CCCCCGCTTA ATACTGACGC TCTCGCACCC ATCTCTCTCC TTCTAGCCTC
  541 CGCTAGTCAA AATTTTTGGC GTACTCACCA GTCGCCGCCC CTCGCCTCTT GCCGTGCGCG
  601 CTTCAGCAAG CCGAGTCCTG CGTCGAGAGA GCTCCTCTGG TTTCCCTTTC GCTTTCAAGT
  661 CCCTGTTCGG GCGCCACTGC TAGAGATTTT CCACACTGAC TAAAAGGGTC TGAGGGATCT
  721 CTAGTTACCA GAGTCACACA ACAGACGGGC ACACACTACT TGAAGCACTC AAGGCAAGCT
  781 TTATTGAGGC TTAAGCAGTG GGTTCCCTAG TTAGCCAGAG AGCTCCCAGG CTCAGATCTG
  841 GTCTAACCAG AGAGACCCAG TACAGGCAAA ACGCGCTGCT TATATAGACC TCCCACCGTA
  901 CACGCCTACC GCCCATTTGC GTCAATGGGG GCGGAGTTGT TACGACATTT TTGGGAAAGT
  961 CCCGTTTGAT TTTGGGTGCC CAAAACAAAC TCCCATTTGA CGTCAATGGG GGTGGAGACT
 1021 TTGGGAAATC CCCGGTGAGT CAAACCCGCT ATCCACGCCC ATGATGTTAC TTGCGAAACG
1081 GCATCACATT GTATTAGCCG AATGACTAAA TACCGTTAGA AATG
//
Primer: BGH_rev2 RDB07991_A3G2c_v2.seq
>06386_07991_A3G2_BGH_rev2_F07_16.ab1
    1 CGATGCCACG GGTTAAGTAA GCCCGGGGGG GGGGCAAACA ACAGATGGCT GGCAACTAGA
   61 AGGCACAGTC GAGGCTGATC AGCGGGTTTA AACGGGCCCT GCTAGAGATT TTCCACACTG
  121 ACTAAAAGGG TCTGAGGGAT CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA
  181 CTTGAAGCAC TCAAGGCAAG CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG
  241 AGAGCTCCCA GGCTCAGATC TGGTCTAACC AGAGAGACCC AGTACAGTCC GGATGCAGCT
  301 CTCGGGCCAT GTGATGAAAT GCTAGGCGGC TGTCAAACCT CCACTCTAAT ACTTCTCTCT
  361 CCGGGTCATC CATCCCATGC AGGCTCACAG GGTGTAACAA GCGGGTGTTC TCTCCTTCAT
  421 TGGCTTCTTC TACCTTCTCT TGCTCAACTG GTACTAGCTT GTAGCACCAT CCAAAGGTCA
  481 GTGGATATCT GATCCCTGGC CCTGGTGTGT AGTTCTGCCA ATCAGGGAAG TAGCCTTGTG
  541 TGTGGTAGAT CCACAGATCA AGGATATCTT GTCTTCGTTG GGAGTGAATT AGCCCTTCCA
  601 GTCCCCCCTT TTCTTTTAAA AAGTGGCTAA GATCTACAGC TGCCTTGTAA GTCATTGGTC
  661 TTAAAGGTAC CTGAGGTGTG ACTGGAAAAC CCACCTCCTC CTCCTCTTGT GCTTCTAGCC
  721 AGGCACAATC AGCATTGGTA GCTGCTGTAT TGCTACTTGT GATTGCTCCA TGTTTTTCTA
  781 GGTCTCGACG GGTATCGATG CGGGGAGGCG GCCCAAAGGG AGATCCGACT CGTCTGAGGG
  841 CGAAGGCGAA GACGCGGAAG AGGCCGCAGA GCCGGCAGCA GGCCGCGGGA AGGAAGGGTC
  901 CGCTGGATTG AGGGCCGAAA GGGACGTAGC AAAAGGACGT CCCGCGCAGA ATCCAGGTGG
  961 CAACACAGGC GAGCAGCCAT GGAAAAGGAC GTCAGCTTCC CCCGGACAAC ACCAACGGAA
 1021 ATTGTTCAGT GGCCAAACAG GCCGGAGCCT CTTGTCCAGC CAGCCGGGGC AAAGGCAAGG
1081 CGGCCGATTG AAGTCGTCCG TGGCAAATAG GGAGGGGGGG AAAGCCGTGA AAA
//
Primer: SV40pro_F_V2 RDB07991_A3G2d_v2.seq
>06386_07991_A3G2_SV40pro_F_V2_D03_12.ab1
    1 CAGCACCTTT TTGGAGCTAG GCTTTTGCAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC
   61 GGATCTGATC AGCACGTGTT GACAATTAAT CATCGGCATA GTATATCGGC ATAGTATAAT
  121 ACGACAAGGT GAGGAACTAA ACCATGGCCA AGTTGACCAG TGCCGTTCCG GTGCTCACCG
  181 CGCGCGACGT CGCCGGAGCG GTCGAGTTCT GGACCGACCG GCTCGGGTTC TCCCGGGACT
  241 TCGTGGAGGA CGACTTCGCC GGTGTGGTCC GGGACGACGT GACCCTGTTC ATCAGCGCGG
  301 TCCAGGACCA GGTGGTGCCG GACAACACCC TGGCCTGGGT GTGGGTGCGC GGCCTGGACG
  361 AGCTGTACGC CGAGTGGTCG GAGGTCGTGT CCACGAACTT CCGGGACGCC TCCGGGCCGG
  421 CCATGACCGA GATCGGCGAG CAGCCGTGGG GGCGGGAGTT CGCCCTGCGC GACCCGGCCG
  481 GCAACTGCGT GCACTTCGTG GCCGAGGAGC AGGACTGACA CGTGCTACGA GATTTCGATT
  541 CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA
  601 TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG
  661 CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT
  721 TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA
  781 TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA
  841 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT
  901 GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC
  961 AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG
 1021 GGTTTGCGTA TTGGGCGCTC TTTCCGCTTT CTCGCTCACT GACTCGCTGC GCTCGGTCGG
 1081 TTCGGCCTGC GGCGGAGCGG GTATCAGCTC ACTCAAAGCG TAATACCGTA ATCACAGAAT
1141 CAGGGATACG CTAGGAAGGA TCATGCTGTG AAAGTCA
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

References

user report Murakami-Tonami, Y., SGO1 is involved in the DNA damage response in MYCN-amplified neuroblastoma cells. Sci. Rep. 6: 31615 (2016). PMID 27539729.

2017.10.23

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