Please review the QC test results indicated by icon below as well as clone information before placing your order.

CSII-CMV-mRFP1

Lentivirus vector plasmid of mRFP1 driven by CMV promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering	Please visit Ordering instruction.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Remarks	((Remarks)) Remember that you will be working with samples containing infectious virus. Please contact us. We will provide you with appropriate documents. This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。 提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
備考	【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB07991_A3G2p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: PRE_rev () Region: PRE,mRFP1,CMV pro.2 Sequence file: RDB07991_A3G2a.seq
>06386_07991_A3G2_PRE_rev_D07_10.ab1     1 AGTATCAAAT AGCGTAAAGG AGCAACATAG TTAAGAATAC CAGTCAATCT TTCACAAATT    61 TTGTAATCCA GAGGTTGATT ATCGATAAGC TTGATCCCTC GATGTTAACT CTAGAGGATC   121 CGCGGCCGCG AATTCTTAGG CGCCGGTGGA GTGGCGGCCC TCGGCGCGCT CGTACTGTTC   181 CACGATGGTG TAGTCCTCGT TGTGGGAGGT GATGTCCAGC TTGATGTCGG TCTTGTAGGC   241 GCCGGGCAGC TGCACGGGCT TCTTGGCCAT GTAGGTGGTC TTGACCTCGG CGTCGTAGTG   301 GCCGCCGTCC TTCAGCTTCA GCCTCATCTT GATCTCGCCC TTCAGGGCGC CGTCCTCGGG   361 GTACATCCGC TCGGTGGAGG CCTCCCAGCC CATGGTCTTC TTCTGCATTA CGGGGCCGTC   421 GGAGGGGAAG TTGGTGCCGC GCAGCTTCAC CTTGTAGATG AACTCGCCGT CCTGCAGGGA   481 GGAGTCCTGG GTCACGGTCA CCACGCCGCC GTCCTCGAAG TTCATCACGC GCTCCCACTT   541 GAAGCCCTCG GGGAAGGACA GCTTCAAGTA GTCGGGGATG TCGGCGGGGT GCTTCACGTA   601 GGCCTTGGAG CCGTACTGGA ACTGAGGGGA CAGGATGTCC CAGGCGAAGG GCAGGGGGCC   661 GCCCTTGGTC ACCTTCAGCT TGGCGGTCTG GGTGCCCTCG TAGGGGCGGC CCTCGCCCTC   721 GCCCTCGATC TCGAACTCGT GGCCGTTCAC GGAGCCCTCC ATGCGCACCT TGAAGCGCAT   781 GAACTCCTTG ATGACGTCCT CGGAGGAGGC CATCGGATCC AGTCGAGACC GGTAGCGCTA   841 GCGGATCTGA CGGTTCACTA AACCAGCTCT GCTTATATAG ACCTCCCACC GTACACGCCT   901 ACCGCCCATT TGCGTCAATG GGGGCGGAGT TGTTTACGAC ATTTTTGGAA AGTCCCGTTT   961 GATTTTTGGG TGCCCAAAAC AAACTCCCAT TTGACGTCAA TGGGGGTGGA GACTTTGGGA  1021 AATCCCCGTG AGTCAAACCG CTATCCACGC CCAATTGGAT GTACTGGCCT AAACGCATTC 1081 ACCAATGGTA ATAGCCGAAT GCACCTTAAA TAACG //
Primer: HIVgp_R1 () Region: RRE,HIV-1psi,5'LTR,CMV pro.1 Sequence file: RDB07991_A3G2b.seq
>06386_07991_A3G2_HIVgp_R1_E07_13.ab1     1 CGGCCATCTA TTCCCACTGC TCTTTTTTCT CTCTGCACCA CTCTTCTCTT TGCCTTGGTG    61 GGTGCTACTC CTAATGGTTC AATTTTTACT ACTTTATATT TATATAATTC ACTTCTCCAA   121 TTGTCCCTCA TATCTCCTCC TCCAGGTCTG AAGATCAGCG GCCGGCCGCT TGCTGTGCGG   181 TGGTCTTACT TTTGTTTTGC TCTTCCTCTA TCTTGTCTAA AGCTTCCTTG GTGTCTTTTA   241 TCTCTATCCT TTGATGCACA CAATAGAGGG TTGCTACTGT ATTATATAAT GATCTAAGTT   301 CTTCTGATCC TGTCTGAAGG GATGGTTGTA GCTGTCCCAG TATTTGTCTA CAGCCTTCTG   361 ATGTTTCTAA CAGGCCAGGA TTAACTGCGA ATCGTTCTAG CTCCCTGCTT GCCCATACTA   421 TATGTTTTAA TTTATATTTT TTCTTTCCCC CTGGCCTTAA CCGAATTTTT TCCCATCGCG   481 ATCTAATTCT CCCCCGCTTA ATACTGACGC TCTCGCACCC ATCTCTCTCC TTCTAGCCTC   541 CGCTAGTCAA AATTTTTGGC GTACTCACCA GTCGCCGCCC CTCGCCTCTT GCCGTGCGCG   601 CTTCAGCAAG CCGAGTCCTG CGTCGAGAGA GCTCCTCTGG TTTCCCTTTC GCTTTCAAGT   661 CCCTGTTCGG GCGCCACTGC TAGAGATTTT CCACACTGAC TAAAAGGGTC TGAGGGATCT   721 CTAGTTACCA GAGTCACACA ACAGACGGGC ACACACTACT TGAAGCACTC AAGGCAAGCT   781 TTATTGAGGC TTAAGCAGTG GGTTCCCTAG TTAGCCAGAG AGCTCCCAGG CTCAGATCTG   841 GTCTAACCAG AGAGACCCAG TACAGGCAAA ACGCGCTGCT TATATAGACC TCCCACCGTA   901 CACGCCTACC GCCCATTTGC GTCAATGGGG GCGGAGTTGT TACGACATTT TTGGGAAAGT   961 CCCGTTTGAT TTTGGGTGCC CAAAACAAAC TCCCATTTGA CGTCAATGGG GGTGGAGACT  1021 TTGGGAAATC CCCGGTGAGT CAAACCCGCT ATCCACGCCC ATGATGTTAC TTGCGAAACG 1081 GCATCACATT GTATTAGCCG AATGACTAAA TACCGTTAGA AATG //
Primer: BGH_rev2 () Region: bGHp(A),3'LTR,PRE Sequence file: RDB07991_A3G2c.seq
>06386_07991_A3G2_BGH_rev2_F07_16.ab1     1 CGATGCCACG GGTTAAGTAA GCCCGGGGGG GGGGCAAACA ACAGATGGCT GGCAACTAGA    61 AGGCACAGTC GAGGCTGATC AGCGGGTTTA AACGGGCCCT GCTAGAGATT TTCCACACTG   121 ACTAAAAGGG TCTGAGGGAT CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA   181 CTTGAAGCAC TCAAGGCAAG CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG   241 AGAGCTCCCA GGCTCAGATC TGGTCTAACC AGAGAGACCC AGTACAGTCC GGATGCAGCT   301 CTCGGGCCAT GTGATGAAAT GCTAGGCGGC TGTCAAACCT CCACTCTAAT ACTTCTCTCT   361 CCGGGTCATC CATCCCATGC AGGCTCACAG GGTGTAACAA GCGGGTGTTC TCTCCTTCAT   421 TGGCTTCTTC TACCTTCTCT TGCTCAACTG GTACTAGCTT GTAGCACCAT CCAAAGGTCA   481 GTGGATATCT GATCCCTGGC CCTGGTGTGT AGTTCTGCCA ATCAGGGAAG TAGCCTTGTG   541 TGTGGTAGAT CCACAGATCA AGGATATCTT GTCTTCGTTG GGAGTGAATT AGCCCTTCCA   601 GTCCCCCCTT TTCTTTTAAA AAGTGGCTAA GATCTACAGC TGCCTTGTAA GTCATTGGTC   661 TTAAAGGTAC CTGAGGTGTG ACTGGAAAAC CCACCTCCTC CTCCTCTTGT GCTTCTAGCC   721 AGGCACAATC AGCATTGGTA GCTGCTGTAT TGCTACTTGT GATTGCTCCA TGTTTTTCTA   781 GGTCTCGACG GGTATCGATG CGGGGAGGCG GCCCAAAGGG AGATCCGACT CGTCTGAGGG   841 CGAAGGCGAA GACGCGGAAG AGGCCGCAGA GCCGGCAGCA GGCCGCGGGA AGGAAGGGTC   901 CGCTGGATTG AGGGCCGAAA GGGACGTAGC AAAAGGACGT CCCGCGCAGA ATCCAGGTGG   961 CAACACAGGC GAGCAGCCAT GGAAAAGGAC GTCAGCTTCC CCCGGACAAC ACCAACGGAA  1021 ATTGTTCAGT GGCCAAACAG GCCGGAGCCT CTTGTCCAGC CAGCCGGGGC AAAGGCAAGG 1081 CGGCCGATTG AAGTCGTCCG TGGCAAATAG GGAGGGGGGG AAAGCCGTGA AAA //
Primer: SV40pro_F () Region: SV40pro/ori,Zeo,SV40p(A) Sequence file: RDB07991_A3G2d.seq
>06386_07991_A3G2_SV40pro_F_E02_14_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN ANNNGTGAGG ANGNTTTTTT GGAGGCCTAG GCTTTTGCAA    61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAGCACGTGT TGACAATTAA   121 TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA AACCATGGCC   181 AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG TCGCCGGAGC GGTCGAGTTC   241 TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG ACGACTTCGC CGGTGTGGTC   301 CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC AGGTGGTGCC GGACAACACC   361 CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG CCGAGTGGTC GGAGGTCGTG   421 TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG AGATCGGCGA GCAGCCGTGG   481 GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG TGCACTTCGT GGCCGAGGAG   541 CAGGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC   601 TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG   661 GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT   721 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC   781 AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA NCTCTAGCTA GAGCTTGNCG   841 TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCNCACAAC   901 ATACNANCCG GAANCATAAA GTGTAAAGCC NGGGGNGNCN NATGAGTGAG CTNACTCACA   961 TTAATTGCGT TGNNCNCNCN GCCNGCTTTC CAGNNNGGAA ANCNGNCGNG NNNNCNGNNT 1021 TAATGNNNCN NNNNNCGNNC GGGGNNANNN GNNTNGNNNN NNNGNNNNNN NNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.02

GNP_filter3_RDBDEP_html_230501.pl