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CSII-CMV-mRFP1

Lentivirus vector plasmid of mRFP1 driven by CMV promoter.

Catalog number RDB06386
Resource name CSII-CMV-mRFP1
Clone info. Lentivirus vector plasmid of mRFP1 driven by CMV promoter.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone CSII-CMV-MCS (Plasmid)
Selectable markers Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Gene/insert name Discosoma sp. RFP cDNA
Depositor|Developer Miyoshi, Hiroyuki |
Other clones in our bank

External Database
Discosoma sp. RFP

            Reference sequence
              
             
            Sequence RDB06386z.seq
            Remarks, protocol and/or map (pdf) RDB06386.pdf

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
            Ordering
            Order form [Credit Card Payment]  [Bank Transfer Payment]
            MTA, for use for not-for-profit academic purpose)[Word]
            Please visit Ordering instruction.[link] 
            Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
            Please contact us. We will provide you with appropriate documents.
            This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
            提供案内 (日本国内) [open/close]

            提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
            提供依頼
            提供依頼書 [Word]
            提供同意書 (MTA, 非営利機関による非営利学術研究用)[Word]
            遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
            手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
            備考 【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
            Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06386 CSII-CMV-mRFP1 DNA solution

            Ordering instruction of plasmids [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The CSII-CMV-mRFP1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06386).

            Reference section:

            Further references such as user reports and related articles (go to bottom)


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            Featured content

            Featured content Lentiviral plasmid list (English text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB17472_A9Hlp1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: mCharry-check_R (Pr0521)
            Region: mRFP1 5',CMV pro.2
            Sequence file: RDB17472_A9Hla.seq check
            >06386_17572_A9Hl_2_mCherry-check_R_B01_02_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNCNNCCN NCCTCGNANT TCATCACGCG CTCCCACTTG
               61 AAGCCCTCGG GGAANGNCAG CTTCAAGTAG TCGGGGATGT CGGCGGGGTG CTTCACGTAG
              121 GCCTTGGAGC CGTACTGGAA CTGAGGGGAC AGGATGTCCC AGGCGAAGGG CAGGGGGCCG
              181 CCCTTGGTCA CCTTCAGCTT GGCGGTCTGG GTGCCCTCGT AGGGGCGGCC CTCGCCCTCG
              241 CCCTCGATCT CGAACTCGTG GCCGTTCACG GAGCCCTCCA TGCGCACCTT GAAGCGCATG
              301 AACTCCTTGA TGACGTCCTC GGAGGAGGCC ATCGGATCCA GTCGAGACCG GTAGCGCTAG
              361 CGGATCTGAC GGTTCACTAA ACCAGCTCTG CTTATATAGA CCTCCCACCG TACACGCCTA
              421 CCGCCCATTT GCGTCAATGG GGCGGAGTTG TTACGACATT TTGGAAAGTC CCGTTGATTT
              481 TGGTGCCAAA ACAAACTCCC ATTGACGTCA ATGGGGTGGA GACTTGGAAA TCCCCGTGAG
              541 TCAAACCGCT ATCCACGCCC ATTGATGTAC TGCNAAAACC GCATCACCAT GGTAATAGCG
              601 ATGANTAATA CGTAGANGTA CTGCCAAGTA NGAAAGTCCC ATAANGTCAT GTACTGGGNA
              661 TAATGNCAGG CNGGNCCANT TTACCGTCAN TGNNNTCNAT NNGGGGGCGN ANNTNGGCNN
            721 ATGNNNNNCN NNNN
            //
            Primer: PRE_rev (Pr0250)
            Region: PRE,mRFP1 3',
            Sequence file: RDB17472_A9Hlb.seq check
            >06386_17472_A9Hl_2_PRE_rev_G01_07_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNGNNCA NCATAGTTAA GANNACCAGT CAATCTTTCA
               61 CAAATTTTGT AATCCAGAGG TTGATTATCG ATAAGCTTGA TCCCTCGATG TTAACTCTAG
              121 AGGATCCGCG GCCGCGAATT CTTAGGCGCC GGTGGAGTGG CGGCCCTCGG CGCGCTCGTA
              181 CTGTTCCACG ATGGTGTAGT CCTCGTTGTG GGAGGTGATG TCCAGCTTGA TGTCGGTCTT
              241 GTAGGCGCCG GGCAGCTGCA CGGGCTTCTT GGCCATGTAG GTGGTCTTGA CCTCGGCGTC
              301 GTAGTGGCCG CCGTCCTTCA GCTTCAGCCT CATCTTGATC TCGCCCTTCA GGGCGCCGTC
              361 CTCGGGGTAC ATCCGCTCGG TGGAGGCCTC CCAGCCCATG GTCTTCTTCT GCATTACGGG
              421 GCCGTCGGAG GGGAAGTTGG TGCCGCGCAG CTTCACCTTG TAGATGAACT CGCCGTCCTG
              481 CAGGGAGGAG TCCTGGGTCA CGGTCACCAC GCCGCCGTCC TCGAAGTTCA TCACGCGCTC
              541 CCACTTGAAG CCCTCGGGGA AGGACAGCTT CNAGTAGTCG GGGATGTCGG CGGGGTGCTT
              601 CACGTANGCC TTGGAGCCGT ACTGGAACTG AGGGGACAGG ATGTCNNNNN CGAAGGGCAN
              661 GGGGCCGCCC TTGGTCNCCT TCAGCTTGGC GGTCTGGGTG CCCTCGTANG GGGCGGNCCT
              721 CGCCCTCGCC CTCNATCTCG AACTCGTGGC CGTTNNNNGN AGNCCTCCAT GCGCACCTTG
              781 NANCGCATGN ANCTCCTTNN NTGNNNNNCC TCNNANNNNN GNCNNNNNNA NNNNNNTCNA
              841 GACCGGTNNN NGCNNANCNG NNCNNNNNGN NNNNNNAANN NNNNNNCNGC TNATATANNA
            901 NNTCCCANNG TANANNNNNN NNNCGNNNNN NTTNNNNTNN NNNNNNNN
            //
            Primer: env_F (Pr0258)
            Region: cPPT_CTS,CMV en.2,CMV pro.2
            Sequence file: RDB17472_A9Hlc.seq check
            >06386_17472_A9Hl_2_env_F_F01_06_ABI08.ab1
                1 NNNNNNNNNN NNNNTTCGNN NANTNNNNGN NCTACANATG GCAGTATTCA TCCACAATTT
               61 TAAAAGAAAA GGGGGGNNTG GGGGGTACAG TGCAGGGGAA AGAATAGTAG ACATAATAGC
              121 AACAGACATA CAAACTAAAG AATTACAAAA ACAAATTACA AAAATTCAAA ATTTTCGGGT
              181 TTATTACAGG GACAGCAGAA ATTCACTTTG AATTAATTCG ATTAATAGTA ATCAATTACG
              241 GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC
              301 CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC
              361 ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT
              421 GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT
              481 GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT
              541 TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC
              601 ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGNNTTC CAAGTCTCCA CCCCATTGAC
              661 GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC
              721 TCCGCCCCAT TGACGCAAAT GGGCGGTANG CGTGTACGGT GNGAGGTCTA TATAANCAGA
              781 NCTGGTTTAN TGAACCGTCA NANCCGCTAG CGCNACCGGT CTCGANNNGA TCCNANGGCC
              841 TCCTCCGANG ANGTCATCNN GGANTTCATG CNCTNNNNGN GCGCNNNGGA NGGNNNCNNG
            901 AANNGNNNNN ANNNCNNNNN CNNNNNNNNG NNNANNNNNN NNNN
            //
            Primer: HIV-B_R (Pr0673)
            Region: HIV-1 psi,5' LTR,CMV pro.1,CMV en.1
            Sequence file: RDB17472_A9Hld.seq check
            >06386_17472_A9Hl_HIV-B_R_B01_04_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NAAANTTTTG NNGTANTCNC CNGTCGCCGC CCCTCGCCTC
               61 TTGCCGTGCG CGCTTCAGCA AGCCGAGTCC TGCGTCGAGA GAGCTCCTCT GGTTTCCCTT
              121 TCGCTTTCAA GTCCCTGTTC GGGCGCCACT GCTAGAGATT TTCCACACTG ACTAAAAGGG
              181 TCTGAGGGAT CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC
              241 TCAAGGCAAG CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA
              301 GGCTCAGATC TGGTCTAACC AGAGAGACCC AGTACAGGCA AAACGCGCTG CTTATATAGA
              361 CCTCCCACCG TACACGCCTA CCGCCCATTT GCGTCAATGG GGCGGAGTTG TTACGACATT
              421 TTGGAAAGTC CCGTTGATTT TGGTGCCAAA ACAAACTCCC ATTGACGTCA ATGGGGTGGA
              481 GACTTGGAAA TCCCCGTGAG TCAAACCGCT ATCCACGCCC ATTGATGTAC TGCCAAAACC
              541 GCATCACCAT GGTAATAGCG ATGACTAATA CGTAGATGTA CTGCCAAGTA GGAAAGTCCC
              601 ATAAGGTCAT GTACTGGGCA TAATGCCAGG CGGGCCATTT ACCGTCATTG ACGTCAATAG
              661 GGGGCGTACT TGGCATATGA TACACTTGAT GTACTGCCAA GTGGGCAGTT TANCGTAAAT
              721 ACTCCACCCA TTGACGTCAA TGGAAAGTCC CTATTGGCGT TACTATGGGA ACATACGTCA
              781 TTATTGACGT CAATGGGCGG GGGTCGTTGG GCGGTCAGCC AGGCGGGNCA TTTACCGTAA
              841 GTTATGTAAC GCGGAANTCC NTATATGGNC TATGAACTAA TGANCNCGNN ATTGANTACT
              901 ATTAATNNCT AGTCAATAAT CAATGTCANC GCGNATATCT NGNCCGNACA TCGCGAANCA
              961 NCGCAAAANG CCNAANCCTA ANNNNANNNN NCATGCNNNN GNNGGTCNNN NNNNGNCNNN
            1021 NNNGNANCNN NANNTTNNNN NNNNNN
            //
            Primer: RRE-M_R (Pr0651)
            Region: RRE,HIV-1 psi
            Sequence file: RDB17472_A9Hle.seq check
            >06386_17472_A9Hl_RRE-M_R_C01_07_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNGNN GCTGTTGCGC CTCNATAGCC CTCAGCAAAT TGTTCTGCTG
               61 CTGCACTATA CCAGACAATA ATTGTCTGGC CTGTACCGTC AGCGTCATTG ACGCTGCGCC
              121 CATAGTGCTT CCTGCTGCTC CCAAGAACCC AAGGAACAAA GCTCCTATTC CCACTGCTCT
              181 TTTTTCTCTC TGCACCACTC TTCTCTTTGC CTTGGTGGGT GCTACTCCTA ATGGTTCAAT
              241 TTTTACTACT TTATATTTAT ATAATTCACT TCTCCAATTG TCCCTCATAT CTCCTCCTCC
              301 AGGTCTGAAG ATCAGCGGCC GGCCGCTTGC TGTGCGGTGG TCTTACTTTT GTTTTGCTCT
              361 TCCTCTATCT TGTCTAAAGC TTCCTTGGTG TCTTTTATCT CTATCCTTTG ATGCACACAA
              421 TAGAGGGTTG CTACTGTATT ATATAATGAT CTAAGTTCTT CTGATCCTGT CTGAAGGGAT
              481 GGTTGTAGCT GTCCCAGTAT TTGTCTACAG CCTTCTGATG TTTCTAACAG GCCAGGATTA
              541 ACTGCGAATC GTTCTAGCTC CCTGCTTGCC CATACTATAT GTTTTAATTT ATATTTTTTC
              601 TTTCCCCCTG GCCTTAACCG AATTTTTTCC CATCGCGATC TAATTCTCCC CCGCTTAATA
              661 CTGACGCTCT CGCACCCATC TCTCTCCTTC TAGCCTCCGC TAGTCAAAAT TTTTGGCGTA
              721 CTCACCAGTC GCCGCCNCTC GCCTCTTGCC GTGCGCGCTT CAGCAAGCCG AGTCCTGCGT
              781 CGAGAGAGCT CCTCTGGTTT CNNTTTCGCT TTCAAGTCCC TGTTCGGGCG CCACTGCTAG
              841 AGANTTTNCA CACTGACTAA AANGGTCTGA NGGANCTCTA GTTANCAGAN TCACACAACA
              901 GANGGGCNCN CACTACTTGA ANCNNTCAAN GNAAGCTTTA NTGAGGNTTA ANCAGNGGGT
              961 TCCNNANTNA GCCNNANANN NCCNANGNNC NNNNNNGGNC TNNNNNGANA NNNNNNNTNN
            1021 NNGNNAANNN NNNTNNNNNN ATNNNNNNNC NNNNNNNNNN
            //
            Primer: BGH_rev2 (Pr0606)
            Region: bGH pA,3' LTR
            Sequence file: RDB17472_A9Hlf.seq check
            >06386_17472_A9Hl_2_BGH_rev2_G02_20_ABI24.ab1
                1 NNNNNNNNNN NNNNGGGGGN NGGNNNNNNA CAGATGGCTG GCAACTAGAA GGCACAGTCG
               61 AGGCTGATCA GCGGGTTTAA ACGGGCCCTG CTAGAGATTT TCCACACTGA CTAAAAGGGT
              121 CTGAGGGATC TCTAGTTACC AGAGTCACAC AACAGACGGG CACACACTAC TTGAAGCACT
              181 CAAGGCAAGC TTTATTGAGG CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA GAGCTCCCAG
              241 GCTCAGATCT GGTCTAACCA GAGAGACCCA GTACAGTCCG GATGCAGCTC TCGGGCCATG
              301 TGATGAAATG CTAGGCGGCT GTCAAACCTC CACTCTAATA CTTCTCTCTC CGGGTCATCC
              361 ATCCCATGCA GGCTCACAGG GTGTAACAAG CGGGTGTTCT CTCCTTCATT GGCTTCTTCT
              421 ACCTTCTCTT GCTCAACTGG TACTAGCTTG TAGCACCATC CAAAGGTCAG TGGATATCTG
              481 ATCCCTGGCC CTGGTGTGTA GTTCTGCCAA TCAGGGAAGT AGCCTTGTGT GTGGTAGATC
              541 CACAGATCAA GGATATCTTG TCTTCGTTGG GAGTGAATTA GCCCTTCCAG TCCCCCCTTT
              601 TCTTTTAAAA AGTGGCTAAG ATCTACAGCT GCCTTGTAAG TCATTGGTCT TAAAGGTACC
              661 TGANGTGTGA CTGGAAAACC CACCTCCTCC TCCTCTTGTG CTTCTAGCCA GGCACAATCA
              721 GCATTGGTAG CTGCTGTATT GCTACTTGTG NNTGCTCCAT GTTTTTCTAG GTCTCGACGG
              781 TATCGATGCG GGGNNGCGGC CCAAANGGAN ATCCGANTCG TCTGANGGCG AANGCGAANA
              841 NGCGGAANAN GNNGCANNNC NNNCAGCNNN NCNCGGGAAN GNANNNNCCN CNNGANNNAN
            901 GNNNNNANNN NNNNANCANA NNNNNNTCCC NNNNNNNNNN
            //
            Primer: Zeo-M_R (Pr0675)
            Region: Zeo,SV40 pro_ori
            Sequence file: RDB17472_A9Hlg.seq check
            >06386_17472_A9Hl_2_Zeo-M_R_H02_23_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNGC NNNNCCNCNC CCNGGCNNGG GNGTTGTCCG GCACCNCCTG
               61 GTCCTGGACC GCGCTGATGA ACAGGGTCAC GTCGTCCCGG ACCACACCGG CGAAGTCGTC
              121 CTCCACGAAG TCCCGGGNGA ACCCGAGCCG GTCGGTCCAG AACTCGACCG CTCCGGCGAC
              181 GTCGCGCGCG GTGAGCACCG GAACGGCACT GGTCAACTTG GCCATGGTTT AGTTCCTCAC
              241 CTTGTCGTAT TATACTATGC CGATATACTA TGCCGATGAT TAATTGTCAA CACGTGCTGA
              301 TCAGATCCGA AAATGGATAT ACAAGCTCCC GGGAGCTTTT TGCAAAAGCC TAGGCCTCCA
              361 AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCAG AGGCGGCCTC GGCCTCTGCA
              421 TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG
              481 GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT
              541 TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG
              601 AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC
              661 TGACACACAT TCCACAGAAT TAATTCGCGT TAAATTTTTG TTAAATCAGC TCATTTTTTA
              721 ACCAATANGC CGAAATCGGC AAAATCCCTT ATAAATCAAA AGAATAGACC GAGATANGGT
              781 TGANTGTTGT TCCAGTTTGG AACAAGANTC CACTATTAAA GAACGTGNAN TCCAACGTCN
              841 AANNGNCGAA AAANCGTCTA TCNNGNCGAN GGNCCNCTAC NNNNANCATC ACCNNNNNNN
            901 NNTTTTTTNG GGNNCNANNN GNNNNNNNNA CTNANNNNNN NNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            user_report Kakizawa, S., Functional maintenance of calcium store by ShcB adaptor protein in cerebellar Purkinje cells. Sci. Rep. 10 (1): 14475 (2020). PMID 32879382. [link to RRC of NBRP]
            user_report Murakami-Tonami, Y., SGO1 is involved in the DNA damage response in MYCN-amplified neuroblastoma cells. Sci. Rep. 6: 31615 (2016). PMID 27539729. [link to RRC of NBRP]
            user_report Neri, S., Podoplanin-expressing cancer-associated fibroblasts lead and enhance the local invasion of cancer cells in lung adenocarcinoma. Int. J. Cancer. 137 (4): 784-796 (2015). PMID 25648219. [link to RRC of NBRP]

            2023.12.06

            GNP_filter3_RDBDEP_html_231027.pl