Resource data sheet
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CSII-CMV-mRFP1

Lentivirus vector construct of mRFP1 driven by CMV promoter.

Catalog number RDB06386
Resource name CSII-CMV-mRFP1
Clone info. Lentivirus vector construct of mRFP1 driven by CMV promoter.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-CMV-MCS (Plasmid)
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name Discosoma sp. RFP cDNA
Originator Miyoshi, Hiroyuki |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi as the Developer.
Ordering Please visit Ordering instruction. Please contact us. UCSD's MTA is also required.
Order form 
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Material Transfer Agreement (MTA) [Word]
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06386 CSII-CMV-mRFP1 DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06386z.seq from Depositor
Electronic file PDF (map, protocol and/or remarks) RDB06386.pdf from Depositor

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB07991_A3G2p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: PRE,mRFP1,CMV pro.2 | Primer: PRE_rev | Sequence: RDB07991_A3G2a.seq
>06386_07991_A3G2_PRE_rev_D07_10.ab1
    1 AGTATCAAAT AGCGTAAAGG AGCAACATAG TTAAGAATAC CAGTCAATCT TTCACAAATT
   61 TTGTAATCCA GAGGTTGATT ATCGATAAGC TTGATCCCTC GATGTTAACT CTAGAGGATC
  121 CGCGGCCGCG AATTCTTAGG CGCCGGTGGA GTGGCGGCCC TCGGCGCGCT CGTACTGTTC
  181 CACGATGGTG TAGTCCTCGT TGTGGGAGGT GATGTCCAGC TTGATGTCGG TCTTGTAGGC
  241 GCCGGGCAGC TGCACGGGCT TCTTGGCCAT GTAGGTGGTC TTGACCTCGG CGTCGTAGTG
  301 GCCGCCGTCC TTCAGCTTCA GCCTCATCTT GATCTCGCCC TTCAGGGCGC CGTCCTCGGG
  361 GTACATCCGC TCGGTGGAGG CCTCCCAGCC CATGGTCTTC TTCTGCATTA CGGGGCCGTC
  421 GGAGGGGAAG TTGGTGCCGC GCAGCTTCAC CTTGTAGATG AACTCGCCGT CCTGCAGGGA
  481 GGAGTCCTGG GTCACGGTCA CCACGCCGCC GTCCTCGAAG TTCATCACGC GCTCCCACTT
  541 GAAGCCCTCG GGGAAGGACA GCTTCAAGTA GTCGGGGATG TCGGCGGGGT GCTTCACGTA
  601 GGCCTTGGAG CCGTACTGGA ACTGAGGGGA CAGGATGTCC CAGGCGAAGG GCAGGGGGCC
  661 GCCCTTGGTC ACCTTCAGCT TGGCGGTCTG GGTGCCCTCG TAGGGGCGGC CCTCGCCCTC
  721 GCCCTCGATC TCGAACTCGT GGCCGTTCAC GGAGCCCTCC ATGCGCACCT TGAAGCGCAT
  781 GAACTCCTTG ATGACGTCCT CGGAGGAGGC CATCGGATCC AGTCGAGACC GGTAGCGCTA
  841 GCGGATCTGA CGGTTCACTA AACCAGCTCT GCTTATATAG ACCTCCCACC GTACACGCCT
  901 ACCGCCCATT TGCGTCAATG GGGGCGGAGT TGTTTACGAC ATTTTTGGAA AGTCCCGTTT
  961 GATTTTTGGG TGCCCAAAAC AAACTCCCAT TTGACGTCAA TGGGGGTGGA GACTTTGGGA
 1021 AATCCCCGTG AGTCAAACCG CTATCCACGC CCAATTGGAT GTACTGGCCT AAACGCATTC
1081 ACCAATGGTA ATAGCCGAAT GCACCTTAAA TAACG
//
Region: RRE,HIV-1psi,5'LTR,CMV pro.1 | Primer: HIVgp_R1 | Sequence: RDB07991_A3G2b.seq
>06386_07991_A3G2_HIVgp_R1_E07_13.ab1
    1 CGGCCATCTA TTCCCACTGC TCTTTTTTCT CTCTGCACCA CTCTTCTCTT TGCCTTGGTG
   61 GGTGCTACTC CTAATGGTTC AATTTTTACT ACTTTATATT TATATAATTC ACTTCTCCAA
  121 TTGTCCCTCA TATCTCCTCC TCCAGGTCTG AAGATCAGCG GCCGGCCGCT TGCTGTGCGG
  181 TGGTCTTACT TTTGTTTTGC TCTTCCTCTA TCTTGTCTAA AGCTTCCTTG GTGTCTTTTA
  241 TCTCTATCCT TTGATGCACA CAATAGAGGG TTGCTACTGT ATTATATAAT GATCTAAGTT
  301 CTTCTGATCC TGTCTGAAGG GATGGTTGTA GCTGTCCCAG TATTTGTCTA CAGCCTTCTG
  361 ATGTTTCTAA CAGGCCAGGA TTAACTGCGA ATCGTTCTAG CTCCCTGCTT GCCCATACTA
  421 TATGTTTTAA TTTATATTTT TTCTTTCCCC CTGGCCTTAA CCGAATTTTT TCCCATCGCG
  481 ATCTAATTCT CCCCCGCTTA ATACTGACGC TCTCGCACCC ATCTCTCTCC TTCTAGCCTC
  541 CGCTAGTCAA AATTTTTGGC GTACTCACCA GTCGCCGCCC CTCGCCTCTT GCCGTGCGCG
  601 CTTCAGCAAG CCGAGTCCTG CGTCGAGAGA GCTCCTCTGG TTTCCCTTTC GCTTTCAAGT
  661 CCCTGTTCGG GCGCCACTGC TAGAGATTTT CCACACTGAC TAAAAGGGTC TGAGGGATCT
  721 CTAGTTACCA GAGTCACACA ACAGACGGGC ACACACTACT TGAAGCACTC AAGGCAAGCT
  781 TTATTGAGGC TTAAGCAGTG GGTTCCCTAG TTAGCCAGAG AGCTCCCAGG CTCAGATCTG
  841 GTCTAACCAG AGAGACCCAG TACAGGCAAA ACGCGCTGCT TATATAGACC TCCCACCGTA
  901 CACGCCTACC GCCCATTTGC GTCAATGGGG GCGGAGTTGT TACGACATTT TTGGGAAAGT
  961 CCCGTTTGAT TTTGGGTGCC CAAAACAAAC TCCCATTTGA CGTCAATGGG GGTGGAGACT
 1021 TTGGGAAATC CCCGGTGAGT CAAACCCGCT ATCCACGCCC ATGATGTTAC TTGCGAAACG
1081 GCATCACATT GTATTAGCCG AATGACTAAA TACCGTTAGA AATG
//
Region: bGHp(A),3'LTR,PRE | Primer: BGH_rev2 | Sequence: RDB07991_A3G2c.seq
>06386_07991_A3G2_BGH_rev2_F07_16.ab1
    1 CGATGCCACG GGTTAAGTAA GCCCGGGGGG GGGGCAAACA ACAGATGGCT GGCAACTAGA
   61 AGGCACAGTC GAGGCTGATC AGCGGGTTTA AACGGGCCCT GCTAGAGATT TTCCACACTG
  121 ACTAAAAGGG TCTGAGGGAT CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA
  181 CTTGAAGCAC TCAAGGCAAG CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG
  241 AGAGCTCCCA GGCTCAGATC TGGTCTAACC AGAGAGACCC AGTACAGTCC GGATGCAGCT
  301 CTCGGGCCAT GTGATGAAAT GCTAGGCGGC TGTCAAACCT CCACTCTAAT ACTTCTCTCT
  361 CCGGGTCATC CATCCCATGC AGGCTCACAG GGTGTAACAA GCGGGTGTTC TCTCCTTCAT
  421 TGGCTTCTTC TACCTTCTCT TGCTCAACTG GTACTAGCTT GTAGCACCAT CCAAAGGTCA
  481 GTGGATATCT GATCCCTGGC CCTGGTGTGT AGTTCTGCCA ATCAGGGAAG TAGCCTTGTG
  541 TGTGGTAGAT CCACAGATCA AGGATATCTT GTCTTCGTTG GGAGTGAATT AGCCCTTCCA
  601 GTCCCCCCTT TTCTTTTAAA AAGTGGCTAA GATCTACAGC TGCCTTGTAA GTCATTGGTC
  661 TTAAAGGTAC CTGAGGTGTG ACTGGAAAAC CCACCTCCTC CTCCTCTTGT GCTTCTAGCC
  721 AGGCACAATC AGCATTGGTA GCTGCTGTAT TGCTACTTGT GATTGCTCCA TGTTTTTCTA
  781 GGTCTCGACG GGTATCGATG CGGGGAGGCG GCCCAAAGGG AGATCCGACT CGTCTGAGGG
  841 CGAAGGCGAA GACGCGGAAG AGGCCGCAGA GCCGGCAGCA GGCCGCGGGA AGGAAGGGTC
  901 CGCTGGATTG AGGGCCGAAA GGGACGTAGC AAAAGGACGT CCCGCGCAGA ATCCAGGTGG
  961 CAACACAGGC GAGCAGCCAT GGAAAAGGAC GTCAGCTTCC CCCGGACAAC ACCAACGGAA
 1021 ATTGTTCAGT GGCCAAACAG GCCGGAGCCT CTTGTCCAGC CAGCCGGGGC AAAGGCAAGG
1081 CGGCCGATTG AAGTCGTCCG TGGCAAATAG GGAGGGGGGG AAAGCCGTGA AAA
//
Region: SV40pro/ori,Zeo,SV40p(A) | Primer: SV40pro_F | Sequence: RDB07991_A3G2d.seq
>06386_07991_A3G2_SV40pro_F_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN ANNNGTGAGG ANGNTTTTTT GGAGGCCTAG GCTTTTGCAA
   61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAGCACGTGT TGACAATTAA
  121 TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA AACCATGGCC
  181 AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG TCGCCGGAGC GGTCGAGTTC
  241 TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG ACGACTTCGC CGGTGTGGTC
  301 CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC AGGTGGTGCC GGACAACACC
  361 CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG CCGAGTGGTC GGAGGTCGTG
  421 TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG AGATCGGCGA GCAGCCGTGG
  481 GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG TGCACTTCGT GGCCGAGGAG
  541 CAGGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC
  601 TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG
  661 GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT
  721 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC
  781 AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA NCTCTAGCTA GAGCTTGNCG
  841 TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCNCACAAC
  901 ATACNANCCG GAANCATAAA GTGTAAAGCC NGGGGNGNCN NATGAGTGAG CTNACTCACA
  961 TTAATTGCGT TGNNCNCNCN GCCNGCTTTC CAGNNNGGAA ANCNGNCGNG NNNNCNGNNT
 1021 TAATGNNNCN NNNNNCGNNC GGGGNNANNN GNNTNGNNNN NNNGNNNNNN NNNNNNNNNN
 1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1141 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

user report Murakami-Tonami, Y., SGO1 is involved in the DNA damage response in MYCN-amplified neuroblastoma cells. Sci. Rep. 6: 31615 (2016). PMID 27539729.
user report Neri, S., Podoplanin-expressing cancer-associated fibroblasts lead and enhance the local invasion of cancer cells in lung adenocarcinoma. Int. J. Cancer. 137 (4): 784-796 (2015). PMID 25648219.

2019.08.28

GNP_filter3_RDBDEP_html_190816.pl