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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCMFlag_hsNR5A1

Expression vector of human AD4BP.

Catalog number RDB06299
Resource name pCMFlag_hsNR5A1
Alternative name SET-0097_13
Clone info. Expression vector of human AD4BP. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment Derived from Sugano BC032501. PCR cloning, forward primer: gactattcgtacgacgagga; reverse primer: tcaagtctgcttggcttgca.
Vector backbone pCMV_S-FLAG (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human SF-1; AD4BP cDNA
Depositor|Developer DNA Bank, |
Other clones in our bank

External Database
Human SF-1; AD4BP

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL and cite Gene 200, 149-156, 1997 in any publication.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 発表に際し、理研BRCから入手したことを明記し、指定する文献(Gene, 200, 149-156 (1997))を引用すること。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB06299 pCMFlag_hsNR5A1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB06299.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content pCMV_tag and pRSV_tag assay-ready clones (English text)
          Featured content Dnaconda's recommendation EXR0078e (English text)
          Featured content Dnaconda's recommendation EXR0078j (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12861_A4Iqp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro, T7 pro, FLAG, insert 5'
          Sequence file: RDB12861_A4Iqd.seq check
          >06299_12861_A4Iq_CMV-Foward_A04_01.ab1
              1 NNNNNNNNNN NNNNNNNNNN NGNNNNNNNG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
             61 ATAAGCAGAG CTCTCTGGCT AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT
            121 ACGACTCACT ATAGGGAGAC CCAAGCTGAA TTCACCATGG ACTACAAGGA CGATGACGAT
            181 AAGGCGGCCG ACTATTCGTA CGACGAGGAC CTGGACGAGC TGTGCCCCGT GTGCGGGGAC
            241 AAGGTGTCCG GCTACCACTA CGGACTGCTC ACGTGTGAGA GCTGCAAGGG CTTCTTCAAG
            301 CGCACGGTGC AGAACAACAA GCACTACACG TGCACCGAGA GCCAGAGCTG CAAGATCGAC
            361 AAGACGCAGC GCAAGCGCTG TCCCTTCTGC CGCTTCCAGA AATGCCTGAC GGTGGGGATG
            421 CGCCTGGAAG CCGTGCGCGC TGACCGTATG AGGGGTGGCC GGAACAAGTT TGGGCCGATG
            481 TACAAGCGGG ACCGGGCCCT GAAACAGCAG AAGAAGGCAC AGATTCGGGC CAATGGCTTC
            541 AAGCTGGAGA CAGGGCCCCC GATGGGGGTG CCCCCGCCGC CCCCTCCCGC ACCGGACTAC
            601 GTGCTGCCTC CCAGCCTGCA TGGGCCTGAG CCCAAGGGCC TGGCCGCCGG TCCACCTGCT
            661 GGGCCACTGG GCGACTTTGG GGCCCCAGCA CTGCCCATGG CCGTGCCCGG TGCCCACGGG
            721 CCACTGGCTG GCTACCTCTA CCCTGCCTTT CCTGGCCGTG CCATCNAGTC TGANTACCCG
            781 GANCCTTATG CCAGCCCCCC ACAGCCTGGG CTGCCGTACN GCTACCCAGA GCCCTTCTCT
            841 GGANGGCCCN ACNNGCCTGA NCTCATCNTG CANCTGCTGC NGCTGGANCC NGATGANGAN
            901 CANGNNNGGG NCCGCATCTT GGGNTGCCNG NNNNANCCNN CCAAAAGCCG CCCCNANCAN
            961 NNNNNNGNNN TNNNNNNTNC TGNNNNNNAN NNNCNNANCN NANCNTTCNN NNNCNNNNNN
          1021 NNNN
          //
          Primer: BGH_rev2
          Region: bGH p(A), SP6 pro, insert 3'
          Sequence file: RDB12861_A4Iqe.seq check
          >06299_12861_B0CdS_BGH_rev2_D05_04_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNG GNNGNNGGGN NAACAACAGA TGGCTGGCAA CTAGAAGGCA
             61 CAGTCGAGGC TGATCAGCGA GCTCTAGCAT TTAGGTGACA CTATAGAATA GGGCCCTCTA
            121 GATGCATGCT CGAGCGGCCT CAAGTCTGCT TGGCTTGCAG CATTTCGATG AGCAGGTTGT
            181 TGCGGGGCAT CTCGTTGCCC AGGTGCTTGT GGTACAGGTA CTCCTTGGCC TGCATGCTCA
            241 GGGCCCGCAC CTCCACCAGG CACAGCAGCA GCTGCTGGAA TTTGTCCCCG CAGTGCGGGT
            301 AGTGGCACAG GGTGTAGTCA AGCAGGGCGG CGTTGGCCTT CTCCTGAGCG TCTTTCACCA
            361 GGATGTGGTT ATTCAGGAAC TTCAAATCCA GGCTGAAGAG GATGATGAAC TTGAGGCAGA
            421 CAAACTCCTG CCGGTCCAGC TGCAGCGCAA GCAGCTGCAG CACCAGCTCC TGCGCCCGCA
            481 ACACCAGGCT GTGCAGCAGC GAGCCCGCCT GGGTGGCCAC TGTGGTCAGC TCCACCTCCT
            541 GCCCGGTGAC CAGCAGGATG CTGCCCTCCT TGCCGTGCTG GACCTGGCGG TAGATGTGGT
            601 CGAACACCAG CAGCTCGCTC CAGCAGTTCT GCAGCAGCGT CATCTGGTCG GCCACCTCCA
            661 GCTCCTTGAA GACCATGCAC CTGCGTGCCC AGTCCACGAT GGAGATGAAG GTCTGGTCGG
            721 CCATTCTGCA CAGGANGCCG AANGCCGCCG GCTGGTCGGG GCGGCTTTTG GTGGGCTCCT
            781 GCAGGCAGCC CAAGATGCGG GCCCGCACCT GGTCCTCATC CGGCTNCNGC TGCAGCAGCT
            841 GCANGANGAN CTCNNNCACG TNGGGCCCTC CAGANAANNG NNTCTGGGTA NCCNTACGGC
            901 AGCCCAGGCT GTGGGGGGCN NGNNTNANNN TNCNGGNANT CNNANTTGAT GGCNCNGNCC
          961 NNNAAANNNN NGNNANNNNN NGNNNNNNNN NN
          //
          Primer: SV40pro_ori_F
          Region: SV40 pro_ori, NeoR
          Sequence file: RDB12861_A4Iqf.seq check
          >06299_12861_B0CdS_SV40pro_ori_F_E05_05_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNC NNCTCGNCCT CTGAGCTATT CNNGAAGTAG TGAGGAGGCT
             61 TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGGATATCC ATTTTCGGAT
            121 CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG
            181 GTTCTCCGGC CGCTTGNGNG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG
            241 GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA
            301 AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC
            361 TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG
            421 ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG
            481 CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA
            541 CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG
            601 CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC
            661 TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG
            721 ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG
            781 GCCGGCTGGG TGTGGCGGAN CGCTATCNNA CATAGCGTTG GCTANCCGTG ATATTGCTGA
            841 AGANCTTGNN GGNNAANGGG CTNACCGCTT NCNCGTGCTT TACNGNATCG CCGCTCCCGA
            901 NTCNNANCGC ATCGCCNTCN NTCNNNTNCT TGANNANTTN TNNTGANCNG NNNTNTGGGG
            961 TTCNAAATGA NCGANCNAGC GNNNCCNNNN NTGNNNTCNN NNNNTTNNNA NNCNNNNNNN
          1021 NNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          user_report Yamauchi, K., Overexpression of Nuclear Receptor 5A1 Induces and Maintains an Intermediate State of Conversion between Primed and Naive Pluripotency. Stem Cell Reports 14 (3): 506-519 (2020). PMID 32084386.
          user_report Matsuo, K., Significance of dopamine D1 receptor signalling for steroidogenic differentiation of human induced pluripotent stem cells. Sci. Rep. 7 (1): 15120 (2017). PMID 29123220.
          user_report Sonoyama, T.,, Differentiation of human embryonic stem cells and human induced pluripotent stem cells into steroid-producing cells. Endocrinology, 153 (9): 4336-4345 (2012). PMID 22778223.

          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl