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pCMFlag_hsCREB3L1 (#RDB06267)

Expression vector of human CREB3L1.

Alternative name SET-0079_187
Clone info. Expression vector of human CREB3L1. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment PCR cloning, forward primer: gacgccgtcttggaacccttc; reverse primer: ctaggagagtttgatggtggtgt.
Vector backbone pCMV_S-FLAG (RDB05956) (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human Oasis cDNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB06267.pdf

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RDB06267 pCMFlag_hsCREB3L1 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMFlag_hsCREB3L1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06267).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15506_A7Ksp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB15506_A7Ksa.seq check
>06267_15506_A7Ks_CMV-Forward_A09_03_ABI24.ab1
    1 CTTTAACTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
  121 CTCACTATAG GGAGACCCAA GCTGAATTCA CCATGGACTA CAAGGACGAT GACGATAAGG
  181 CGGCCGACGC CGTCTTGGAA CCCTTCCCGG CCGACAGGCT GTTCCCCGGA TCCAGCTTCC
  241 TGGACTTGGG GGATCTGAAC GAGTCGGACT TCCTCAACAA TGCGCACTTT CCTGAGCACC
  301 TGGACCACTT TACGGAGAAC ATGGAGGACT TCTCCAATGA CCTGTTCAGC AGCTTCTTTG
  361 ATGACCCTGT GCTGGATGAG AAGAGCCCTC TATTGGACAT GGAACTGGAC TCCCCTACGC
  421 CAGGCATCCA GGCGGAGCAC AGCTACTCCC TGAGCGGCGA CTCAGCGCCC CAGAGCCCCC
  481 TTGTGCCCAT CAAGATGGAG GACACCACCC AAGATGCAGA GCATGGAGCA TGGGCGCTGG
  541 GACACAAACT GTGCTCCATC ATGGTGAAGC AGGAGCAGAG CCCGGAGCTG CCCGTGGACC
  601 CTCTGGCTGC CCCCTCGGCC ATGGCTGCCG CGGCCGCCAT GGCCACCACC CCGCTGCTGG
  661 GCCTCAGCCC CTTGTCCAGG CTGCCCATCC CCCACCAGGC CCCGGGAGAG ATGACTCAGC
  721 TGCCAGTGAT CAAAGCAGAG CCTCTGGAGG TGAACCAGTT CCTCAAAGTG ACACCGGAGG
  781 ACCTGGTGCA GATGCCTCCG ACGCCCCCCA GCAGCCATGG CAGTGACAGC GACGGCTCCC
  841 AGAGTCCCCG CTCTCTGCCC CCCCTCCAGC CCTGTCAGGC CCATGGCGCG CTCCTCCACG
  901 GCCATCTCCA CCTCCCCACT CCTCACTGCC CCTCACAAAT TACAGGGGGA CATCAGGGCC
  961 CACTGCTCCT GACAGAGGAG GAGAAGCGGG ACCCTGATTG CTGAGGGCTA CCCCATCCCC
 1021 CCACAAAACT CCCCCCTCAC CAAAGCCCGA GGGAGAAGGC CCTTGAGGAG AGTCCCGGAG
 1081 GAAAAATCAA GAGCAGATCT CAGCCCAGGA AGAGTCGTTC GTTAAGAAAG GAGATATGCT
1141 GCAAGCTGAT TCCTAGGGAG CCA
//
Primer: BGH_rev2
Sequence file: RDB15506_A7Ksb.seq check
>06267_15506_A7Ks_BGH_rev2_B09_06_ABI24.ab1
    1 CATTCAGTCA GAGCACGGGG GAGGGGCAAC AACAGATGGC TGGCAACTAG AAGGCACAGT
   61 CGAGGCTGAT CAGCGAGCTC TAGCATTTAG GTGACACTAT AGAATAGGGC CCTCTAGATG
  121 CATGCTCGAG CGGCCCTAGG AGAGTTTGAT GGTGGTGTTG GGGCCCAGAT CCCTGTCGTG
  181 GAACCACTCC TTGGAGTGGG AGAAGTCGGG GCTGGTGCCG TTTCCACCGT CTTTAGGCCA
  241 GGCCTCACTC AGGTACTTGG TGGTCTCGTG GGTGCTGTCC AGGTGATCAT GCTGCAGGTG
  301 GTCCCGGGGC CGCTGCTCTG CCGGCCCCCC GGGGTTGATT TCCCAGCCAT CTGGGGGCTC
  361 CATGGGCAGC AGGGTGCTGC GGCCATCTTC CCATAAGCCT GCCCCGTCAT CGTAGAATAG
  421 GAGGCTTCGG GAGGGCATCT GGCTGGCCGT GTAGACGCCG TCTGCGGCCA GGGGGTCTTC
  481 CTTCACAGTC TGGGAGCCGG AGGAGAACTC GGGAAGGCAG GGCACGAGGG AGCCCAGCAC
  541 CAGAACAAAG CACAAGGCTG CCACCATGAG GCAGGTCCCA GTCTGGGTGG CGGCCATCTT
  601 GTAAGGTCTG GAGATCTTGT TGGTGACCAG AGTCTGGAGT TTCTGCAGCT GCTGGAGCAG
  661 GGTCCTGTTG GCATTCTCCA GGGTCTCCAC CTTCTTCCAC AGTTCATTGT TCTCAGATGT
  721 AAATGTCTCC ACCTTCTTTT CTAGACACTC CACATACTCC TTCTTCTTAC GACGGCTCTC
  781 CTGGGCTGAG ATCTTGTTCT TGATTTTCCT CCGGACTCTC TTCAAGGCCT TCTCCTCGGC
  841 TTTGGTGAGG GGGAGTTTTG TGGGGGAATG GGGGTAGCCC TCAGCAATCA GGGGTCCCGC
  901 TTTCTCCTCC TCTGTCAGAG CAGTGGGCCC TGATGTCCCC CTGTAATTTG TGAGGGGCAG
961 TGAGAAGTGG GGGAGAGGGT TGGAAGAATG GCC
//
Primer: SV40pro_F_V2
Sequence file: RDB15506_A7Ksc.seq check
>06267_15506_A7Ks_SV40pro_F_V2_C09_09_ABI24.ab1
    1 GTAGCAAACT TTTTTTGGAG GCCTAGGCTT TTGCAAAGCT CCCGGGAGCT TGGATATCCA
   61 TTTTCGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT
  121 TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC
  181 AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC
  241 TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC
  301 TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG
  361 CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC
  421 TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG
  481 ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC
  541 GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC
  601 CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA
  661 CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA
  721 TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG
  781 ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG
  841 CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG
  901 GACTCTGGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCCATC ACGAGATTTC
  961 GATTCCACCG CCGCCTTTCT ATGGAAAGGT TGGGGCTTCG GAATCGTTTT CCGGACGCCG
 1021 GCTGGGATGA TCCTTCCAGC GCGGGGGATC TCATGCTGGA GGTTTCTCGC CCACCCGACC
 1081 TTGATTTATT GCCAGCCTAT ATGTACCATT AAGGCCATTA GGCCATTCGA ACAAAAATGT
1141 TT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user_report Oh-Hashi, K., Transcriptional regulation of mouse mesencephalic astrocyte-derived neurotrophic factor in Neuro2a cells. Cell. Mol. Biol. Lett., 18 (3): 398-415 (2013). PMID 23864333. [link to RRC of NBRP]