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pCMFlag_hsCREB3L1

Expression vector of human CREB3L1.

Catalog number RDB06267
Resource name pCMFlag_hsCREB3L1
Alternative name SET-0079_187
Clone info. Expression vector of human CREB3L1. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment PCR cloning, forward primer: gacgccgtcttggaacccttc; reverse primer: ctaggagagtttgatggtggtgt.
Vector backbone pCMV_S-FLAG (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human Oasis cDNA
Depositor|Developer DNA Bank, |
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External Database
Human Oasis

          Reference sequence
            

          Distribution information

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          RDB06267 pCMFlag_hsCREB3L1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB06267.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content pCMV_tag and pRSV_tag assay-ready clones (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15506_A7Ksp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB15506_A7Ksa.seq check
          >06267_15506_A7Ks_CMV-Forward_A09_03_ABI24.ab1
              1 CTTTAACTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
             61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
            121 CTCACTATAG GGAGACCCAA GCTGAATTCA CCATGGACTA CAAGGACGAT GACGATAAGG
            181 CGGCCGACGC CGTCTTGGAA CCCTTCCCGG CCGACAGGCT GTTCCCCGGA TCCAGCTTCC
            241 TGGACTTGGG GGATCTGAAC GAGTCGGACT TCCTCAACAA TGCGCACTTT CCTGAGCACC
            301 TGGACCACTT TACGGAGAAC ATGGAGGACT TCTCCAATGA CCTGTTCAGC AGCTTCTTTG
            361 ATGACCCTGT GCTGGATGAG AAGAGCCCTC TATTGGACAT GGAACTGGAC TCCCCTACGC
            421 CAGGCATCCA GGCGGAGCAC AGCTACTCCC TGAGCGGCGA CTCAGCGCCC CAGAGCCCCC
            481 TTGTGCCCAT CAAGATGGAG GACACCACCC AAGATGCAGA GCATGGAGCA TGGGCGCTGG
            541 GACACAAACT GTGCTCCATC ATGGTGAAGC AGGAGCAGAG CCCGGAGCTG CCCGTGGACC
            601 CTCTGGCTGC CCCCTCGGCC ATGGCTGCCG CGGCCGCCAT GGCCACCACC CCGCTGCTGG
            661 GCCTCAGCCC CTTGTCCAGG CTGCCCATCC CCCACCAGGC CCCGGGAGAG ATGACTCAGC
            721 TGCCAGTGAT CAAAGCAGAG CCTCTGGAGG TGAACCAGTT CCTCAAAGTG ACACCGGAGG
            781 ACCTGGTGCA GATGCCTCCG ACGCCCCCCA GCAGCCATGG CAGTGACAGC GACGGCTCCC
            841 AGAGTCCCCG CTCTCTGCCC CCCCTCCAGC CCTGTCAGGC CCATGGCGCG CTCCTCCACG
            901 GCCATCTCCA CCTCCCCACT CCTCACTGCC CCTCACAAAT TACAGGGGGA CATCAGGGCC
            961 CACTGCTCCT GACAGAGGAG GAGAAGCGGG ACCCTGATTG CTGAGGGCTA CCCCATCCCC
           1021 CCACAAAACT CCCCCCTCAC CAAAGCCCGA GGGAGAAGGC CCTTGAGGAG AGTCCCGGAG
           1081 GAAAAATCAA GAGCAGATCT CAGCCCAGGA AGAGTCGTTC GTTAAGAAAG GAGATATGCT
          1141 GCAAGCTGAT TCCTAGGGAG CCA
          //
          Primer: BGH_rev2
          Sequence file: RDB15506_A7Ksb.seq check
          >06267_15506_A7Ks_BGH_rev2_B09_06_ABI24.ab1
              1 CATTCAGTCA GAGCACGGGG GAGGGGCAAC AACAGATGGC TGGCAACTAG AAGGCACAGT
             61 CGAGGCTGAT CAGCGAGCTC TAGCATTTAG GTGACACTAT AGAATAGGGC CCTCTAGATG
            121 CATGCTCGAG CGGCCCTAGG AGAGTTTGAT GGTGGTGTTG GGGCCCAGAT CCCTGTCGTG
            181 GAACCACTCC TTGGAGTGGG AGAAGTCGGG GCTGGTGCCG TTTCCACCGT CTTTAGGCCA
            241 GGCCTCACTC AGGTACTTGG TGGTCTCGTG GGTGCTGTCC AGGTGATCAT GCTGCAGGTG
            301 GTCCCGGGGC CGCTGCTCTG CCGGCCCCCC GGGGTTGATT TCCCAGCCAT CTGGGGGCTC
            361 CATGGGCAGC AGGGTGCTGC GGCCATCTTC CCATAAGCCT GCCCCGTCAT CGTAGAATAG
            421 GAGGCTTCGG GAGGGCATCT GGCTGGCCGT GTAGACGCCG TCTGCGGCCA GGGGGTCTTC
            481 CTTCACAGTC TGGGAGCCGG AGGAGAACTC GGGAAGGCAG GGCACGAGGG AGCCCAGCAC
            541 CAGAACAAAG CACAAGGCTG CCACCATGAG GCAGGTCCCA GTCTGGGTGG CGGCCATCTT
            601 GTAAGGTCTG GAGATCTTGT TGGTGACCAG AGTCTGGAGT TTCTGCAGCT GCTGGAGCAG
            661 GGTCCTGTTG GCATTCTCCA GGGTCTCCAC CTTCTTCCAC AGTTCATTGT TCTCAGATGT
            721 AAATGTCTCC ACCTTCTTTT CTAGACACTC CACATACTCC TTCTTCTTAC GACGGCTCTC
            781 CTGGGCTGAG ATCTTGTTCT TGATTTTCCT CCGGACTCTC TTCAAGGCCT TCTCCTCGGC
            841 TTTGGTGAGG GGGAGTTTTG TGGGGGAATG GGGGTAGCCC TCAGCAATCA GGGGTCCCGC
            901 TTTCTCCTCC TCTGTCAGAG CAGTGGGCCC TGATGTCCCC CTGTAATTTG TGAGGGGCAG
          961 TGAGAAGTGG GGGAGAGGGT TGGAAGAATG GCC
          //
          Primer: SV40pro_F_V2
          Sequence file: RDB15506_A7Ksc.seq check
          >06267_15506_A7Ks_SV40pro_F_V2_C09_09_ABI24.ab1
              1 GTAGCAAACT TTTTTTGGAG GCCTAGGCTT TTGCAAAGCT CCCGGGAGCT TGGATATCCA
             61 TTTTCGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT
            121 TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC
            181 AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC
            241 TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC
            301 TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG
            361 CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC
            421 TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG
            481 ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC
            541 GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC
            601 CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA
            661 CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA
            721 TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG
            781 ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG
            841 CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG
            901 GACTCTGGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCCATC ACGAGATTTC
            961 GATTCCACCG CCGCCTTTCT ATGGAAAGGT TGGGGCTTCG GAATCGTTTT CCGGACGCCG
           1021 GCTGGGATGA TCCTTCCAGC GCGGGGGATC TCATGCTGGA GGTTTCTCGC CCACCCGACC
           1081 TTGATTTATT GCCAGCCTAT ATGTACCATT AAGGCCATTA GGCCATTCGA ACAAAAATGT
          1141 TT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          user_report Oh-Hashi, K., Transcriptional regulation of mouse mesencephalic astrocyte-derived neurotrophic factor in Neuro2a cells. Cell. Mol. Biol. Lett., 18 (3): 398-415 (2013). PMID 23864333.

          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl