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pCMFlag_hsNR1H4 (#RDB06262)

Expression vector of human NR1H4.

Alternative name SET-0039_3
Clone info. Expression vector of human NR1H4. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment PCR cloning, forward primer: ggatcaaaaatgaatctcattgaa; reverse primer: tcactgcacgtcccagatttc.
Vector backbone pCMV_S-FLAG (RDB05956) (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human FXR cDNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB06262.pdf

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RDB06262 pCMFlag_hsNR1H4 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMFlag_hsNR1H4 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06262).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15372_A7I4p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB15372_A7I4a.seq check
>06262_15372_A7I4_CMV-Forward_C07_07_ABI24.ab1
    1 CTGTCACTCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
  121 TCACTATAGG GAGACCCAAG CTGAATTCAC CATGGACTAC AAGGACGATG ACGATAAGGC
  181 GGCCGGATCA AAAATGAATC TCATTGAACA TTCCCATTTA CCTACCACAG ATGAATTTTC
  241 TTTTTCTGAA AATTTATTTG GTGTTTTAAC AGAACAAGTG GCAGGTCCTC TGGGACAGAA
  301 CCTGGAAGTG GAACCATACT CGCAATACAG CAATGTTCAG TTTCCCCAAG TTCAACCACA
  361 GATTTCCTCG TCATCCTATT ATTCCAACCT GGGTTTCTAC CCCCAGCAGC CTGAAGAGTG
  421 GTACTCTCCT GGAATATATG AACTCAGGCG TATGCCAGCT GAGACTCTCT ACCAGGGAGA
  481 AACTGAGGTA GCAGAGATGC CTGTAACAAA GAAGCCCCGC ATGGGCGCGT CAGCAGGGAG
  541 GATCAAAGGG GATGAGCTGT GTGTTGTTTG TGGAGACAGA GCCTCTGGAT ACCACTATAA
  601 TGCACTGACC TGTGAGGGGT GTAAAGGTTT CTTCAGGAGA AGCATTACCA AAAACGCTGT
  661 GTACAAGTGT AAAAACGGGG GCAACTGTGT GATGGATATG TACATGCGAA GAAAGTGTCA
  721 AGAGTGTCGA CTAAGGAAAT GCAAAGAGAT GGGAATGTTG GCTGAATGCT TGTTAACTGA
  781 AATTCAGTGT AAATCTAAGC GACTGAGAAA AAATGTGAAG CAGCATGCAG ATCAGACCGT
  841 GAATGAAGAC AGTGAAGGTC GTGACTTGCG ACAAGTGACC TCGACAACAA AGTCATGCAG
  901 GGAGAAAACT GAACTCACCC CAGATCAACA GACTCTTCTA CATTTTATTA TGGATTCATA
  961 TAACAACAGA GGATGCCCTC AGGAAATAAC AAATAAAATT TTAAAAGAAG AATTTCAGTG
 1021 CAGAAGAAAA TTTTTCTCAT TTTGACGGGA ATGGCCATGA ATCATGTACA GGGTTCTTTG
1081 GTAGATCACA AAAAAGGCTC ACAAGGCATT TTCAGACATT GGAAACCAAT TGA
//
Primer: BGH_rev2
Sequence file: RDB15372_A7I4b.seq check
>06262_15372_A7I4_BGH_ver2_D07_10_ABI24.ab1
    1 CGATCGGTTA GTAGCACGGG GGAGGGGCAA CAACAGATGG CTGGCAACTA GAAGGCACAG
   61 TCGAGGCTGA TCAGCGAGCT CTAGCATTTA GGTGACACTA TAGAATAGGG CCCTCTAGAT
  121 GCATGCTCGA GCGGCCTCAC TGCACGTCCC AGATTTCACA GAGAAGTGGG GTAAACTTGT
  181 GGTCGTTTAC TCTCCATGAC ATCAGCATCT CAGCGTGGTG ATGATTGAAT GTCCGTAATT
  241 CAGTCAGGCG ACCCAGGAGA CAGGCAAAGT GTTGAGGATT TTCAGGCTGG TGAATCTTAC
  301 ACAACTTTTG TAGCACATCA AGAAGTGGCT CCTGAAGCTT CTCTACTGCC TCTCTATCCT
  361 TTATGTATTG TCTATCTGGA GACAGGATAA CAATTGCTGT AAGCAGAGCA TACTCCTCTT
  421 GAGTCATTTT CAGTTCCCCA ATACTTTTAT AAAAACTAAA CATAGGTGTT ATATATTCAT
  481 CAGAGATACC ACTATTTCGA ATTCTTTCTT CCAATAGGTC AGAATGCCCA GACGGAAGTT
  541 TCTTATTGAA AATCTCAGCT GAACGAAGGA ACATAGCTTC AACCGCAGAC CCTTTCAGCA
  601 AAGCAATCTG GTCTTCATGG TCCAAAGTCT GAAATCCTGG TAGCTTTTTT GTGAATTCTA
  661 CAAGAACCTG TACATGATTG GTTGCCATTT CCGTCAAAAT GAGAAAATTT TCTTCTGCAC
  721 TGAATTCTTC TTTTAAAATT TTATTTGTTA TTTCCTGAGG CATCCTCTGT TTGTTATATG
  781 AATCCATAAT AAAATGTAGA AGAGTCTGTT GATCTGGGGG TGAGTTCAGT TTTCTCCCTG
  841 CATGACTTTG TTGTCGAGGT CACTTGTCGC AAGTCACGAC CTTCACTGTC TTCATTCACG
  901 GTCTGATCTG CATGCTGCTT CACATTTTTT TCTCAGTCGC TTAGATTTAC ACTGAAATTT
  961 CAGTTTAACA AGCATTCAGC CCAACATTCC CATCTCTTGC ATTTCCCTTT AGTCGACACT
 1021 CTTGGACACT TTTCTTCGCA TGTACATATC CATTCACACA GGTGGCCCCC GATTTTTACA
1081 CTTGTACACA GCGATTTTGG TTATTGCCTT CCTCCGTTGA AGAAAAACT
//
Primer: SV40pro_F_V2
Sequence file: RDB15372_A7I4c.seq check
>06262_15372_A7I4_SV40pro_F_V2_E07_13_ABI24.ab1
    1 GTAACAGCTT TTTTTTGGGG GGCCTAAGGG CTTTTGCAAA AAGCTCCCGG GGAAGCTTGG
   61 ATATCCATTT TCGGATCTGA TCAAGAGACA GGGATGAGGA TCGTTTCGCA TGATTGAACA
  121 AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG
  181 GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG
  241 CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC
  301 AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT
  361 CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC
  421 ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA
  481 TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC
  541 ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG
  601 GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGG CATGCCCGAC GGCGAGGATC
  661 TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT
  721 CTGGATTCAT CGACTGTTGG CCGGCTGGGG TGTTGGGCGG ACCGCTATCA GGACATAGCG
  781 GTTGGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGGCTGA CCGCTTTCCT
  841 CGTGCTTTTA CGGTATCGCC GGCTCCCGAT TTCGCAGCGC CATCGCCCTT CTATCGCCTT
  901 TCTTTGACGA GTTCTTCTGA GCGGGGACTC TGGGGTTCGA AATTGACCGA CCAAGCCGAC
  961 GGCCCAACCT GCCATCACGA GATTTTCGAT TCCCACCGCC GCCCTTCTAT GAAAAGGGTT
 1021 GGGCTTTCGG GAAATCGGTT TTTCCTGGGA CGCCGGCCTG GGATGGATCC TTCCCAGCGC
 1081 GGGGATCCTG ATGCCTGGAG GGTTCCTTTC GGCCCAACCC GCAACCTTGT AATTGGGCAG
1141 CCTTTAATAA TTGGCTTCAC AAAATGTGAA AGGAGCCCAG A
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles