Please review the QC test results indicated by 
icon below as well as clone information before placing your order.
提供案内 (日本国内) 
[open/close]
Please review the QC test results indicated by icon below as well as clone information before placing your order.
icon below as well as clone information before placing your order.
pCMFlag_hsFOSL1 (#RDB06261)
Expression vector of human FOSL1.
| Alternative name | SET-0037_8 |
|---|---|
| Clone info. | Expression vector of human FOSL1. Expression was confirmed by Western blotting with anti-FLAG antibody. |
| Comment | PCR cloning, forward primer: atgttccgagacttcgggga; reverse primer: tcacaaagcgaggagggttg. |
| Vector backbone | pCMV_S-FLAG (RDB05956) (Plasmid) |
| Size of vector backbone | 5.5 kb |
| Selectable markers | Amp^r (E. coli), Neo^r (mammalian cell) |
| Gene/insert name | Human Fra1; FOSL1 cDNA |
| Depositor|Developer | DNA Bank, | |
| Remarks, protocol and/or map (pdf) | RDB06261.pdf |
|---|
Distribution information
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Ordering forms | Order form [Credit Card Payment  ] [Bank Transfer Payment] [Example of order form ]MTA, for use for not-for-profit academic purpose [Word ] [Example of MTA ]Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
|---|---|
| Terms and conditions for distribution | The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL in any publication. |
[open/close]| 必要書類 | 提供依頼書 [依頼書の記入例 ]提供同意書 (MTA, 非営利学術目的用)[Word ] [MTAの記入例 ]手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
|---|---|
| MTAに書く使用条件 | 発表に際し、理研BRCから入手したことを明記すること。 |
| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB06261 | pCMFlag_hsFOSL1 | DNA solution |
icon below as well as clone information before placing your order.How to cite this biological resource
Materials & Methods section:
| The pCMFlag_hsFOSL1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06261). |
Reference section:
Further references such as user reports and related articles (go to bottom)
QC test results
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB18651_B1Aip1-2.pdf |
|---|
Nucleotide sequence of a portion of this resource (if available).
|
Primer: CMV_Forward (Pr0016) Region: CMV pro,FLAG,insert 5' Sequence file: RDB18651_B1Aia.seq
|
|---|
|
>06261_18651_B1Ai_1_CMV_Forward_A02_02_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT 61 ATAAGCAGAG CTCTCTGGCT AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT 121 ACGACTCACT ATAGGGAGAC CCAAGCTGAA TTCACCATGG ACTACAAGGA CGATGACGAT 181 AAGGCGGCCA TGTTCCGAGA CTTCGGGGAA CCCGGCCCGA GCTCCGGGAA CGGCGGCGGG 241 TACGGCGGCC CCGCGCAGCC CCCGGCCGCA GCGCAGGCAG CCCAGCAGAA GTTCCACCTG 301 GTGCCAAGCA TCAACACCAT GAGTGGCAGT CAGGAGCTGC AGTGGATGGT ACAGCCTCAT 361 TTCCTGGGGC CCAGCAGTTA CCCCAGGCCT CTGACCTACC CTCAGTACAG CCCCCCACAA 421 CCCCGGCCAG GAGTCATCCG GGCCCTGGGG CCGCCTCCAG GGGTACGTCG AAGGCCTTGT 481 GAACAGATCA GCCCGGAGGA AGAGGAGCGC CGCCGAGTAA GGCGCGAGCG GAACAAGCTG 541 GCTGCGGCCA AGTGCAGGAA CCGGANGAAG GAACTGACCG ACNTCCTGCN NGNGGANACT 601 GACAAACTGG AAGANGAGAA NNCTGGGCTG CNGCGAGAGN NTGANGANCT GCAGAANCAG 661 ANNNNCGCCN ANANCTGGNG CTNGGANNCC CNNCNNACCN NNNTGNNAAN NNCNNGNNNN 721 NNCNNNNNNN GGGGANNNNN NNNN // |
|
Primer: bGH_rev3 (Pr0885) Region: bGHpA,insert 3' Sequence file: RDB18651_B1Aib.seq
|
|
>06261_18651_B1Ai_1_bGH_rev3_B02_05_ABI24.ab1 1 NNNNNNNNNN NNNNNGNNGN NGGGCNNACA ACAGATGGCT GGCAACTAGA AGGCACAGTC 61 GAGGCTGATC AGCGAGCTCT AGCATTTAGG TGACACTATA GAATAGGGCC CTCTAGATGC 121 ATGCTCGAGC GGCCTCACAA AGCGAGGAGG GTTGGAGAGC CAAGGGGGTC AGAGGATGGG 181 TCTCCGCTGC TGCTGCTACT CTTGCGATGA GCTGAGGCAC AAGGCTCAGG AGTGCTGGGG 241 TAGGTGAAGA CCAGGCTGGG GGTGAAAGGA GTTAGGGAGG GTGTGGTCAT GAGTGTGGGG 301 GTGTGCAGTG CCTCAGGTTC AAGCACAGGC CCTGGGGAAA GGGAGATACA AGGTACAGGG 361 CGGCAGGGGG CTGGTGGGCT GCTGGTGCCA CTGGTACTGC CTGTGTCCCC CTCCTTGGCT 421 CCTTCCGGGA TTTTGCAGAT GGGTCGGTGG GCTTCCAGCA CCAGCTCTAG GCGCTCCTTC 481 TGCTTCTGCA GCTCCTCAAT CTCTCGCTGC AGCCCANATT TCTCATCTTC CAGTTTGTCA 541 NNCTCCGCCT GCAGGAAGTC GGTCAGTTCC TTCCTCCGGN TCCTGCACTT GNCCGCANCC 601 AGCTTGTTCC GCTNNNNNNT TANNNNNNGG CGCTCCTCTT NCNNCGGGCT GATCTGNNNN 661 NNAGGNNNNN NACNNNNNNN TGNNNGCNGN CNCNNNNNNC NNNNNNNCTC CNGNNNNNNN 721 NNN // |
|
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,NeoR Sequence file: RDB18651_B1Aic.seq
|
|
>06261_18651_B1Ai_1_SV40pro_ori_F_C02_08_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NCTNNNNNNC TGNNCTATTC NNGAAGTAGT GAGGAGGCTT 61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGGATATCCA TTTTCGGATC 121 TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG 181 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG 241 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA 301 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT 361 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA 421 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC 481 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CNTACGCTTG ATCCGGCTAC 541 CTGCCCATTC GACCACCNAN GCGAAACATC GCATCGAGCG AGCACNTACT CGGATGGAAN 601 CCGGTCTNGN NNNTCANGAN GATNNGGACN AANANCNTCN NGGCTCGCGC CNNCCNNACT 661 GTTCGCNNGG CTNNAGGCGC GNATGCCCNA NNNNCNAAGG NNCTCNTCNT GNCCCNNNGN 721 GANNCNNNNN NNNNCNNAAN NNNNNNNNN // |
Please visit 
Sequencing and PCR primers for primer information.
References
Original, user report and related articles
Resource search
Link
Other resources in our bankExternal Database
Human Fra1; FOSL1
Reference sequence
Tips
Featured content