Sequencing and PCR primers for primer information.
Expression vector of human JUN
| Catalog number | RDB06254 |
|---|---|
| Resource name | pCMFlag_hsJUN |
| Alternative name | SET-0010_37 |
| Clone info. | Expression vector of human JUN. Expression was confirmed by Western blotting with anti-FLAG antibody. F-primer: atgactgcaaagatggaaacg; R-primer: tcaaaatgtttgcaactgctg. |
| Vector backbone | pCMV_S-FLAG (Plasmid) |
| Size of vector backbone | 5.5 kb |
| Selectable markers | Amp^r (E. coli), Neo^r (mammalian cell) |
| Gene/insert name | Human c-jun cDNA |
| Depositor | DNA Bank, | |
| Other clones in our bank | human JUN (NCBI Gene 3725) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL in any publication. |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB06254 | pCMFlag_hsJUN | DNA solution |
Electronic file
| Electronic file | PDF RDB06254.pdf from Depositor |
|---|
Featured content
| Featured content | pCMV_tag and pRSV_tag assay-ready clones (English text) |
|---|
References
User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB08129_A4L8p1.pdf |
|---|
Nucleotide sequence of a portion of this resource (if available).
| Primer: CMV-Forward | RDB08129_A4L8a.seq |
|---|---|
|
>06254_08129_A4L8_CMV-Forward_A10_01.ab1 1 TGTCACTCCG CCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA 61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA 121 CTCACTATAG GGAGACCCAA GCTGAATTCA CCATGGACTA CAAGGACGAT GACGATAAGG 181 CGGCCATGAC TGCAAAGATG GAAACGACCT TCTATGACGA TGCCCTCAAC GCCTCGTTCC 241 TCCCGTCCGA GAGCGGACCT TATGGCTACA GTAACCCCAA GATCCTGAAA CAGAGCATGA 301 CCCTGAACCT GGCCGACCCA GTGGGGAGCC TGAAGCCGCA CCTCCGCGCC AAGAACTCGG 361 ACCTCCTCAC CTCGCCCGAC GTGGGGCTGC TCAAGCTGGC GTCGCCCGAG CTGGAGCGCC 421 TGATAATCCA GTCCAGCAAC GGGCACATCA CCACCACGCC GACCCCCACC CAGTTCCTGT 481 GCCCCAAGAA CGTGACAGAT GAGCAGGAGG GCTTCGCCGA GGGCTTCGTG CGCGCCCTGG 541 CCGAACTGCA CAGCCAGAAC ACGCTGCCCA GCGTCACGTC GGCGGCGCAG CCGGTCAACG 601 GGGCAGGCAT GGTGGCTCCC GCGGTAGCCT CGGTGGCAGG GGGCAGCGGC AGCGGCGGCT 661 TCAGCGCCAG CCTGCACAGC GAGCCGCCGG TCTACGCAAA CCTCAGCAAC TTCAACCCAG 721 GCGCGCTGAG CAGCGGCGGC GGGGCGCCCT CCTACGGCGC GGCCGGCCTG GCCTTTCCCG 781 CGCAACCCCA GCAGCAGCAG CAGCCGCCGC ACCACCTGCC CCAGCAGATG CCCGTGCAGC 841 ACCCGCGGCT GCAGGCCCTG AAGGAGGAGC CTCAGACAGT GCCCGAGATG CCCGGCGAGA 901 CACCGCCCCT GTCCCCCATC GACATGGAGT CCCAGGAGCG GATCAAGGCG GAGAGGAAGC 961 GCATGAGGAA CCGCATCGCT GCCTCCAAGT GCCGAAAAAG GAAGCTGGAG AGAATCGCCC 1021 GGGCTGGGAG GAAAAAAGTG AAAACCTTTG AAAGCTCAGA ACTCGGGAGC TGGCGTCACG 1081 TCAACATGCT CAGGACAGTT GCACAGCTAA ACAGAAAGGT CATGAACCAC CGATTAAACA 1141 AGGTGGGGGT GTC // |
|
| Primer: BGH_rev | RDB08129_A4L8b.seq |
|
>06254_08129_A4L8_BGHrev_B10_04.ab1 1 GCCCTGGCAT TTAGGTGACC TATAGAATAG GGCCCTCTAG ATGCATGCTC GAGCGGCCTC 61 AAAATGTTTG CAACTGCTGC GTTAGCATGA GTTGGCACCC ACTGTTAACG TGGTTCATGA 121 CTTTCTGTTT AAGCTGTGCC ACCTGTTCCC TGAGCATGTT GGCCGTGGAC GCCAGCTCCG 181 AGTTCTGAGC TTTCAAGGTT TTCACTTTTT CCTCCAGCCG GGCGATTCTC TCCAGCTTCC 241 TTTTTCGGCA CTTGGAGGCA GCGATGCGGT TCCTCATGCG CTTCCTCTCC GCCTTGATCC 301 GCTCCTGGGA CTCCATGTCG ATGGGGGACA GGGGCGGTGT CTCGCCGGGC ATCTCGGGCA 361 CTGTCTGAGG CTCCTCCTTC AGGGCCTGCA GCCGCGGGTG CTGCACGGGC ATCTGCTGGG 421 GCAGGTGGTG CGGCGGCTGC TGCTGCTGCT GGGGTTGCGC GGGAAAGGCC AGGCCGGCCG 481 CGCCGTAGGA GGGCGCCCCG CCGCCGCTGC TCAGCGCGCC TGGGTTGAAG TTGCTGAGGT 541 TTGCGTAGAC CGGCGGCTCG CTGTGCAGGC TGGCGCTGAA GCCGCCGCTG CCGCTGCCCC 601 CTGCCACCGA GGCTACCGCG GGAGCCACCA TGCCTGCCCC GTTGACCGGC TGCGCCGCCG 661 ACGTGACGCT GGGCAGCGTG TTCTGGCTGT GCAGTTCGGC CAGGGCGCGC ACGAAGCCCT 721 CGGCGAAGCC CTCCTGCTCA TCTGTCACGT TCTTGGGGCA CAGGAACTGG GTGGGGGTCG 781 GCGTGGTGGT GATGTGCCCG TTGCTGGACT GGATTATCAG GCGCTCCAGC TCGGGCGACG 841 CCAGCTTGAG CAGCCCCACG TCGGGCGAGG TGAGGAGGTC CGAGTTCTTG GCGCGGAGGT 901 GCGGCTTCAG GCTCCCCACT GGGTCGGCCA GGTTCAGGGT CATGCTCTGT TTCAGGATCT 961 TGGGGTTACT GTAGCCATAA AGTCCGCTCT CGGACGGGAG GAACGAGGCG TTGAGGGCAT 1021 CGTCATAGAA GGTCGTTTCC ATCTTTGCAG TCATGGCCGC CTTATCGTCA TCGTCCAGTA 1081 GTCCATGGTT GAATCAGCTT GGGATCTCCT ATAGTGAAGT CGTATGATTT CGATAGCCAG 1141 TTAAGCCAGT GGGGTCTC // |
|
| Primer: SV40pro_F_V2 | RDB08129_A4L8c.seq |
|
>06254_08129_A4L8_SV40pro_F_V2_C10_07.ab1 1 GGCCAGCTTT TTTGGAGGCT AGGCTTTTGC AAAAGCTCCC GGGAGCTTGG ATATCCATTT 61 TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC 121 ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA 181 CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT 241 TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT 301 CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG 361 GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG 421 CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC 481 CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA 541 TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG 601 CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC 661 ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG 721 ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA 781 TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG 841 CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC 901 TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC 961 CACCGCCGCC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCCGGGA CGCCGGCTGG 1021 GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCC AACTTGTTAT 1081 GCAGCTATAT GGTACAATGA GCAATAGCAT CACAACTTCA CAAATTAAGC AATTTTTTTT 1141 CAACTGCCAG TTTCTAAGTG G // |
|
Please visit Sequencing and PCR primers for primer information.
References
2018.10.22
GNP_filter3_RDBDEP_html_181012.pl