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pCMFlag_hsJUN

Expression vector of human JUN.

Catalog number RDB06254
Resource name pCMFlag_hsJUN
Alternative name SET-0010_37
Clone info. Expression vector of human JUN. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment PCR cloning, forward primer: atgactgcaaagatggaaacg; reverse primer: tcaaaatgtttgcaactgctg.
Vector backbone pCMV_S-FLAG (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human c-jun cDNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Human c-jun
          Reference sequence
            
           
          Remarks, protocol and/or map (pdf) RDB06254.pdf

          Distribution information

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          RDB06254 pCMFlag_hsJUN DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pCMFlag_hsJUN was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06254).

          Reference section:

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          Featured content pCMV_tag and pRSV_tag assay-ready clones (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB08129_A1Fgp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,FLAG,insert 5'
          Sequence file: RDB08129_A1Fga.seq check
          >06254_08129_B1AjF_CMV-Forward_C10_07_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNTGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
             61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
            121 CTCACTATAG GGAGACCCAA GCTGAATTCA CCATGGACTA CAAGGACGAT GACGATAAGG
            181 CGGCCATGAC TGCAAAGATG GAAACGACCT TCTATGACGA TGCCCTCAAC GCCTCGTTCC
            241 TCCCGTCCGA GAGCGGACCT TATGGCTACA GTAACCCCAA GATCCTGAAA CAGAGCATGA
            301 CCCTGAACCT GGCCGACCCA GTGGGGAGCC TGAAGCCGCA CCTCCGCGCC AAGAACTCGG
            361 ACCTCCTCAC CTCGCCCGAC GTGGGGCTGC TCAAGCTGGC GTCGCCCGAG CTGGAGCGCC
            421 TGATAATCCA GTCCAGCAAC GGGCACATCA CCACCACGCC GACCCCCACC CAGTTCCTGT
            481 GCCCCAAGAA CGTGACAGAT GAGCAGGAGG GCTTCGCCGA GGGCTTCGTG CGCGCCCTGG
            541 CCGAACTGCA CAGCCAGAAC ACGCTGCCNA GCGTCNCGTC NGCGGCGCAG CCGGTCAACG
            601 GGGCANGCAT GGTGGCTCCN GCGGTAGCCT CGGTGGNANG GGGCANCGGC AGCGGCNGCT
            661 TCNGCGCCNG CCNGNACANC GAGNCNNNNG TCTACNNNAA NNNCNNCNNC NTNNNNCNNN
          721 NCNNNCTGAN NNNNNNNNN
          //
          Primer: bGH_rev3 (Pr0885)
          Region: bGHpA,insert 3'
          Sequence file: RDB08129_A1Fgb.seq check
          >06254_08129_B1AjF_bGH_rev3_C11_08_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNGCAA ACNACAGATG GCTGGCAACT AGAAGGCACA
             61 GTCGAGGCTG ATCAGCGAGC TCTAGCATTT AGGTGACACT ATAGAATAGG GCCCTCTAGA
            121 TGCATGCTCG AGCGGCCTCA AAATGTTTGC AACTGCTGCG TTAGCATGAG TTGGCACCCA
            181 CTGTTAACGT GGTTCATGAC TTTCTGTTTA AGCTGTGCCA CCTGTTCCCT GAGCATGTTG
            241 GCCGTGGACG CCAGCTCCGA GTTCTGAGCT TTCAAGGTTT TCACTTTTTC CTCCAGCCGG
            301 GCGATTCTCT CCAGCTTCCT TTTTCGGCAC TTGGAGGCAG CGATGCGGTT CCTCATGCGC
            361 TTCCTCTCCG CCTTGATCCG CTCCTGGGAC TCCATGTCGA TGGGGGACAG GGGCGGTGTC
            421 TCGCCGGGCA TCTCGGGCAC TGTCTGAGGC TCCTCCTTCA GGGCCTGCAG CCGCGGGTGC
            481 TGCACGGGCA TCTGCTGGGG CAGGTGGTGC GGCGGCTGCT GCTGCTGCTG GGGTTGCGCG
            541 GGAAAGGCCA GGCCGGCCGC GCCGTANGAN GGCGCCCCGC CGCCGCTGCT CNGCGCGNCT
            601 GGGTTGAAGT TGCTGANGTT TGCGTANANC GGCGGCTCGC TGTGCNNGNT GGCGCTNNNN
            661 CNCCGCTGCC GCNGNCCCCN GNCNNCNNNN NNNNNCNNNN CNNNCNNGCN TGNCCCNNTN
          721 ANCNGNTGNN CNNNNNANNT NNNNNNNGNN NNNNNNNN
          //
          Primer: SV40pro_ori_F (Pr0731)
          Region: SV40 pro_ori,NeoR
          Sequence file: RDB08129_A1Fgc.seq check
          >06254_08129_B1AjF_SV40pro_ori_F_C12_09_ABI24.ab1
              1 NNNNNNNNNN NNNNGNCGCC TCNGCCTCTG ANCTATTCCN GAAGTAGTGA GGAGGCTTTT
             61 TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG GATATCCATT TTCGGATCTG
            121 ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT
            181 CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT
            241 GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA
            301 CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG
            361 CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT
            421 GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG
            481 AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT
            541 GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG
            601 GTCTTGTCGA TCANGANGAN CTGNNCNAAN ANCATCNNGG NCTCGCGCCN GCCNAACTGN
            661 TCGCCNGGCT NNNNNNNCGC ATGNCNNANN GCNANNNNNN CGNCNNGNNC NNNNNNNNNG
          721 NNNGNNNNNN NNNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl