RDB06252 - pCMFlag

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pCMFlag_hsBACH2 (#RDB06252)

Expression vector of human BACH2.

Alternative name	SET-0008_5
Clone info.	Expression vector of human BACH2. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment	Derived from Sugano FBR04526. PCR cloning, forward primer: atgtctgtggatgagaagcc; reverse primer: ctaggtataatctttcctgg. Nucleotide substitution A1361G (Glu218Gly) and G2214A (Val352Val) to NM_021813.
Vector backbone	pCMV_S-FLAG (RDB05956) (Plasmid)
Size of vector backbone	5.5 kb
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name	Human BACH2 cDNA
Depositor\|Developer	DNA Bank, \|

Remarks, protocol and/or map (pdf)	RDB06252.pdf

Distribution information

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Terms and conditions for distribution The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL and cite Gene 200, 149-156, 1997 in any publication.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL and cite Gene 200, 149-156, 1997 in any publication.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	発表に際し、理研BRCから入手したことを明記し、指定する文献(Gene, 200, 149-156 (1997))を引用すること。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB06252	pCMFlag_hsBACH2	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMFlag_hsBACH2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06252).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB13753_A6A4p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB13753_A6A4a.seq
>06252_13753_A6A4_CMV-Forward_C09_09.ab1 1 ATAAACCTCC GCCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA 61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG 121 ACTCACTATA GGGAGACCCA AGCTGAATTC ACCATGGACT ACAAGGACGA TGACGATAAG 181 GCGGCCATGT CTGTGGATGA GAAGCCTGAC TCCCCCATGT ATGTGTATGA GTCCACAGTC 241 CACTGCACCA ACATCCTCCT GGGCCTCAAT GACCAGCGGA AAAAGGATAT TCTCTGTGAC 301 GTGACTTTGA TCGTGGAGAG GAAGGAGTTC CGGGCCCACC GGGCTGTGCT GGCCGCATGC 361 AGTGAATATT TTTGGCAGGC GCTGGTTGGA CAGACAAAAA ATGATTTGGT GGTCAGCTTG 421 CCTGAGGAGG TCACAGCCAG GGGCTTTGGG CCGCTGTTAC AGTTTGCCTA CACTGCCAAG 481 CTGTTACTCA GCAGAGAAAA CATCCGCGAG GTCATCCGCT GTGCTGAGTT CCTGCGCATG 541 CACAACCTGG AGGACTCCTG CTTCAGCTTC CTGCAGACCC AGCTCCTGAA CAGTGAGGAT 601 GGCCTGTTTG TGTGCCGGAA GGATGCTGCG TGCCAGCGCC CACACGAGGA CTGCGAGAAC 661 TCTGCAGGAG AGGAGGAGGA TGAAGAGGAG GAGACGATGG ATTCAGAGAC GGCCAAGATG 721 GCTTGCCCCA GGGACCAGAT GCTTCCAGAG CCCATCAGCT TTGAGGCCGC CGCCATCCCC 781 GTAGCAGAGA AGGAAGAAGC CCTGCTGCCC GAGCCTGACG TGCCCACAGA CACCAAGGGG 841 GAGCTCAGAA AAGGACGCGT TAACGCAGTA CCCCAGATAC AAGAATACCA GCTTGCATGT 901 ACCAAGAATG TCTATAATGC ATCATCACAC AGTACCTCAG GTTTTGCAGC ACATTCCCGG 961 GAAGATACTC TAGCAACAGC CTCAAGCCCG GGGCTTGCAG GGGGCAGATA AAAGTGAGCC 1021 GCCCAGTTGA GAGAATTGAG AGAGAGCATC ACGCTCTGGC TGATCTGGAG AATGGAAG //
Primer: BGH_rev Sequence file: RDB13753_A6A4b.seq
>06252_13753_A6A4_BGHrev_A12_03.ab1 1 GTATGGACAT TTTAGGGTGA ACTATAGAAT AGGGCCCTCT AGATGCATGC TCGAGCGGCC 61 CTAGGTATAA TCTTTCCTGG GCTGTTCGTC AGTTGTACAC TTATCAGTCA TTTCCTGGCA 121 GAAGTCCACG GTCACTGTTT GGCTCCTGGG TTCAAGAGGA GGTCCTCTCT CTGAGAAGGT 181 TCCCGGGTCA GTGCCTTCTA GTCTCCTCCC AGAGGTACAA TTCTCGGAGG TGTTGCTGGG 241 TGCCCAGGGG GGTCCGGGGG GAGCCGCGCC TGGCTCCAAG CAGCAGGGCA CGTTTTCCCC 301 CACTGCGCAT TGGGAGGCCG CAATGTTCTG CTCAGCACCC AAGGGCGCAG GGTTAATACT 361 GGAGGCCGTG GGCAAGTCCA TGGGTCTGAG GACAGGGCAA TACCGATGCA GGGCCTGGAT 421 CTGCTCGGGG CTCTGGATGT CTCGGCAAAC TTCCTGGGAA AGGCAGGAGA AGTTGTCCAA 481 CAGTTCCCCC ATGCATGCTT TCAGTTGATT CCTCTCTGAC AACAGTTTCT CTTTCTCACA 541 CACCAATTTG CGGATTTCAC ATTCTAAATT CTGAATACAG TCCAGTTTCC TTTTGCGGCA 601 GCGCTGGGCC GCGATGCGGT TCTTGCTGCG CCGTCGGACA TCATGAATAA ACTCTAACTG 661 TTCTGAGGTT AGCTTGTGCA TTTTAATCAT CATCTGGAAA TCGTTCCTTG GAAGATCTGT 721 GATTTGATCT ACAGGAAAAG GAAGTTTTAC CTCCTGGCCC CTGTCCTGCA CAGGACACGA 781 CTCACTGTCT GCTTCCGAGA ACGATCCGGA TTCGTCACTG GAGTTGGTTC CATAAGACTG 841 CTCACATTTA ATTTGGGGGC CGCACTTGGT TGTACATTCC ATCTCCCATC AGGCCTGGTT 901 TCCTGATGTT CTGTGGCAAG GAAATCTGGC TCCCTGGGGA ACAGGGCGAG GAGGAGAACT 961 CACAGAGAGG GAGGCTGCAG GTGAGCCCCC CCCGCCTCCC GTTCCCTCCG CGTTAGGAAT 1021 AGGAGGAAGC AAGGAGGCTG GCAGTCCCTG ATCCTGGTTC CTCCAAGGGG GGGGGATGA //
Primer: SV40pro_F_V2 Sequence file: RDB13753_A6A4c.seq
>06252_13753_A6A4_SV40pro_F_V2_B12_06.ab1 1 ATTAGGCGGT TTTTTTGGGA GCTAGGGCTT TTGGCAAAAG CTCCCGGGAG CTTGGAATAT 61 CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG 121 GATTGCACGC GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA 181 ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT 241 TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG 301 GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA 361 AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA 421 CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT 481 TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC 541 TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC 601 GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT 661 GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGGA AAATGGGCCG CTTTTCTGGA 721 TTCATCGACT GTGGCCGGCT GGGGTGTGGC GGACCGCTAT CAGGGACAAT AGCGTTGGCT 781 ACCCGTGATA TTGCTGAAGA GCTTGGCGGG CGAATGGGGC TGACCGCTTC CTCGTGCTTT 841 ACGGGTATCG CCGCTCCCCG ATTTCGCAGC GCATCGCCTT TCTATCGCCC TTCTTTGACG 901 AGTTCTTCTG AGCGGGGACC TCTGGGGGTT CGAAAATGGA CCGAACCAAG CGACGCCCCA 961 AACCCTGGCC AATCACGAAG ATTTTCGAAT TCCCAACCGC CCGGCCTTTC TAATGAAAAG 1021 GGTTGGGGGC TTCCGGAATC GGTTTTTCCG GGAACGCCTC GGGGCCTGGG ATTGATTCCC 1081 TTCCGAGCGC CGGGGGATTC TCAATGGCCT GGAGGTTTCT TTCCGCCCCA ACCTCGCTAA 1141 GGCGCATG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

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Other resources in our bank

human BACH2

External Database
Human BACH2

Reference sequence