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pDUAL2-GFH81

S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 (low strength) promoter

Catalog number RDB06204
Resource name pDUAL2-GFH81
Clone info. S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 (low strength) promoter.
Multicopy marker, ura4+; Integration marker, ura4+, leu1-. Gateway incompatible.
Vector backbone pDUAL2 (Plasmid)
Selectable markers Amp^r
Reference of nucleotide sequence AB364070.1 (DDBJ accession number)
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
Please use Schedule A (GFP-A) additionally.
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RDB06204 pDUAL2-GFH81 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06204z.seq from Depositor

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14466_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw RDB14466_A6L7a.seq
>06204_14466_A6L7_nmt1Fw_C06_09_ABI24.ab1
    1 TGCATTTATT AATCAGGAAA CGTAACTCTC GGCTACTGGA TGGTTCAGTC ACCCAACGAT
   61 TACTGGGGAG AGAAAACAGG GCAAAAGCAA AGCTTAAAGG AATCCGATTG TCATTCGGCA
  121 ATGTGCAGCG AAACTAAAAA CCGGATAATG GACCTGTTAA TCGAAACATT GAAGATGGAA
  181 GAGGAATCCT GGCATATCAT CAATTGAATA AGTTGAATTA ATTATTTCAA TCTCATTCTC
  241 ACTTTCTGAC TTATAGTCGC TTTGTTAAAG CTAGCGTCGA CAGGCCTGGA TCCGGATATC
  301 ATGAGTAAAG GAGAAGAACT TTTCACTGGA GTTGTCCCAA TTCTTGTTGA ATTAGATGGT
  361 GATGTTAATG GGCACAAATT TTCTGTCAGT GGAGAGGGTG AAGGTGATGC AACATACGGA
  421 AAACTTACCC TTAAATTTAT TTGCACTACT GGAAAACTAC CTGTTCCGTG GCCAACACTT
  481 GTCACTACTT TCTGTTATGG TGTTCAATGC TTTTCAAGAT ACCCAGATCA CATGAAACAG
  541 CATGACTTTT TCAAGAGTGC CATGCCCGAA GGCTATGTAC AGGAAAGAAC TATATTTTTC
  601 AAAGATGACG GGAACTACAA GACACGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT
  661 AATAGAATCG AGTTAAAAGG TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA
  721 TTGGAATACA ACTATAACTC ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA
  781 ATCAAAGTTA ACTTCAAAAT TAGACACAAC ATTGAAGATG GAAGCGTTCA ACTAGCAGAC
  841 CATTATCAAC AAAATACTCC AATTGGCGAT GGCCCTGTCC TTTTACCAGA CAACCATTAC
  901 CTGTCCACAC AATCTGCCCT TTCGAAAGAT CCCAACGAAA AGAGAGACCA CATGGGTCCC
  961 TTCTTGAGTT GTAACAGCTG CTGGGATTAC ACATGGCATG GATGGACTAT ACAACCCATG
 1021 GACCTACAGA ACGAACGATG ACAACATCAC ACATCACCAC TGAGATTCTC GACTCTAGAG
1081 ATCGGCGCGC CACTTCTAAA ATAAAGGCGG A
//
Primer: ADH_rev RDB14466_A6L7b.seq
>06204_14466_A6L7_ADH_rev_D06_12_ABI24.ab1
    1 GGAGAGGTCT CAGATAGATT TTCGTTTTAA ACCTAAGAGT CACTTTAAAA TTTGTATACA
   61 CTTATTTTTT TTATAACTTA TTTAATAATA AAAATCATAA ATCATAAGAA ATTCGCTTAT
  121 TTAGAAGTGG CGCGCCGGAT CCTCTAGAGT CGAGATCTCA GTGGTGATGG TGGTGATGTT
  181 TGTCATCGTC GTCCTTGTAG TCCATGGGTT TGTATAGTTC ATCCATGCCA TGTGTAATCC
  241 CAGCAGCTGT TACAAACTCA AGAAGGACCA TGTGGTCTCT CTTTTCGTTG GGATCTTTCG
  301 AAAGGGCAGA TTGTGTGGAC AGGTAATGGT TGTCTGGTAA AAGGACAGGG CCATCGCCAA
  361 TTGGAGTATT TTGTTGATAA TGGTCTGCTA GTTGAACGCT TCCATCTTCA ATGTTGTGTC
  421 TAATTTTGAA GTTAACTTTG ATTCCATTCT TTTGTTTGTC TGCCATGATG TATACATTGT
  481 GTGAGTTATA GTTGTATTCC AATTTGTGTC CAAGAATGTT TCCATCTTCT TTAAAATCAA
  541 TACCTTTTAA CTCGATTCTA TTAACAAGGG TATCACCTTC AAACTTGACT TCAGCACGTG
  601 TCTTGTAGTT CCCGTCATCT TTGAAAAATA TAGTTCTTTC CTGTACATAG CCTTCGGGCA
  661 TGGCACTCTT GAAAAAGTCA TGCTGTTTCA TGTGATCTGG GTATCTTGAA AAGCATTGAA
  721 CACCATAACA GAAAGTAGTG ACAAGTGTTG GCCACGGAAC AGGTAGTTTT CCAGTAGTGC
  781 AAATAAATTT AAAGGGTAAG TTTTCCGTAT GTTGCATCAC CCTTCACCCT CTCCACTGAC
  841 AGAAAATTTG TGCCCATTAA CATCACCATC TAATTTCAAC AAGAATTTGG GACAACTCCA
  901 GTGAAAAGTT TCTTCTCCTT TACTCATGAT ATCCCGGGAT CCAGGCCCTG TCGACGCTAG
  961 CTTTAACAAA GCGACTATAG TCAGAAAGTG AGAATGGAGA TGAAATTAAT CATCACTAAT
 1021 CATGGATGAT ATGCCAAGGA ATCCTCATCA ATCCTCAATG TATCCGAATT ACAGGTTGCT
1081 GA
//
Primer: ADH-Forward RDB14466_A6L7c.seq
>06204_14466_A6L7_ADH-Forward_E06_15_ABI24.ab1
    1 TGGTCCTTAT TGACACACCT CTACCGGCAG ATCCGCTAGG GATAACAGGG TAATATACTA
   61 GTGCCTCTAT TGATGCCTAT GGAACCCCTT TGACTGACGA GACTGTGAAG GCTTGTTTGG
  121 AAGCTGACGG TGTTCTTTTG GGTGCCGTTG GTGGTCCTGA ATGGACCAAC CCCAATTGTC
  181 GTCCTGAGCA AGGTTTATTG AAGCTTCGTA AGAGTATGGG TGTTTGGGCC AACCTTCGAC
  241 CTTGCAACTT TGCCAGCAAG TCTTTAGTCA AGTACAGCCC TTTGAAGCCT GAAATCGTTG
  301 AAGGTGTCGA TTTTTGTGTT GTACGAGAAC TTACTGGAGG TTGTTACTTT GGTGAGCGCA
  361 CTGAGGACAA CGGATCGGGT TATGCTATGG ACACTTGGCC TTACAGTTTG GAAGAAGTTT
  421 CTCGTATTGC TCGTTTGGCG GCCGCGGGCC CAACCTTCCA ATTCATTAAA TCGAGTCCTC
  481 CATCAGATGA TTTACAAGGT TTTGCATAGA ATCCCTAAGT AAGAGAAGTT GTTAGTGATT
  541 TCTTCAAGTT TACTAAAATT TACATACAAA TGGATGATCT CTTCTCCATT GTTTCCTTTC
  601 TTCTTGCAAA CGCGTTTTAC AAAGAGATGA CATGCTATTT TCTGATTATT TTTTTCTATA
  661 GTTTTCTATT TTGTACGTCC TTGGGAGTCC GAAATGTAAA ATCGGGGTAT GGTTAGTAGC
  721 GGCAGCGTTC ATTATGGTAT ATAAAAGATA AAAAGTAGGG GCAAGCGAGA CAGAATGGGA
  781 TACAAAGGGC ATCGTCTATA ATTATAGCTA AAAATTGTAT TTTAATTTGT ATTTTTTGTA
  841 ATTTTATTTC TCATTGTTTT ACTTAAAATG AATGCGAAAT TAGAGAAAAC TTCAACGAAA
  901 TGTCAAAATA AGCTCAGCAA AATATACAAT TTTTAGGAAA GCGATCAGCA ACCTTATTCT
  961 CCGTTGTAGA TTCTTTTTTC TTCATAAATT CGACAATGTA ACTGGCAATG GATTTTTTCT
 1021 CTAGATGCTA TTAAATGAAC CTTATCATAT CATAACTCAT GTTCGCATGA TGCTAACTCT
1081 AA
//
Primer: f1ori_R3 RDB14466_A6L7d.seq
>06204_14466_A6L7_f1ori_R3_G01_19_ABI24.ab1
    1 GCAATGGAGC ATGAGTATGC GGTGTGAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC
   61 ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC
  121 TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA
  181 ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CGAGCTCGGG
  241 AGCGCTACCG TGAATGGGCT CAACCAAACA ATGAACCTTT TCTTCTGATT TTCCTACCAC
  301 ACCGGAAAGG GAGGCAGAAG GCAAAAGGCC CAAGCTACCA GGAATGACAG AAGCCTCATC
  361 TGAAATAATG TCACCAAACA AGTTGTCAGT CAAAACAACA CCGTTAAGTG TACGAGGGCT
  421 CTTGACCAAA AGCATGGCTG CGGAGTCAAT GAGCTGGTTT TTTAAGGTAA GGTGAGGATA
  481 TTCCTCCTTA AAAATCTTAG CTACAGTCTT GCGCCAAAGA CGAGAAGTTG CCAAAACATT
  541 AGCTTTGTCG AGTAATGTGA CGGGAGCAGG AGGGTTGGAA GTTTCAGCTA AGCGGCCGCG
  601 GGCCCGAGTC TAACTCCTTA ACCACTCGGA CATAGTGACT TATCTGACAC TCATTGTAAA
  661 TTAATATATA TATAGGCATT TTGTTTAGTT AAAGGTACTT AAGTAATTAG TATAAACGAA
  721 CCAATTTTAT AATCAGGAAG TTAAGTGAAT GGTAGCACAT GTCGTAAAAA TTGTGAATTT
  781 TTATTGAATA ATATTTTAAA TACAAGCCTT TCTAGACTAG GTATACTCAT AAACATATGA
  841 GCAAAAGGAT AGAGGAGATT ACATTGCATC TTCTACAAAT TTATTTATTG CCCTTTACTG
  901 AAAAATTAAA TAATGAGTAC TAAATGATAA AAAGCGCTCA GTACAAGAAA GATTGCAAAA
  961 ATATTGCATT CTTCATGAAA TTAAAGTTGC ATATAAGCAT ATTGAAAGTA ATAGTACTAA
 1021 AACAGCAGTT AGCGAAAATT TATAGAATTA TATTCGCAGA CATTGTGACA TTAAATTTAA
 1081 AAATGTAGAT TTTTTACTAT CTCATTAACG CATGAGCGTT TATAAAACGC TCAATAAGCT
1141 TTAACCATCC TTTTTTTTTT TGCAGT
//
Primer: Reverse2 RDB14466_A6L7e.seq
>06204_14466_A6L7_Reverse2_G06_21_ABI24.ab1
    1 CGTAATGCAT TACGCCAGCT TGTGATATTG ACGAAACTTT TTGACATCTA ATTTATTCTG
   61 TTCCAACACC AATGTTTATA ACCAAGTTTT ATCTTGTTTG TCTACATGGT ATTTTACATT
  121 CATCTACATA CATCTTTCAT TGGCTTTGTA CATAGTTATC ATTACAAGTC TAAAAAAATT
  181 CACTCTTTTC TTATTCAATG TCAATCCAAG AGAAAAGATT GTGGTAATGT TGTAGGAGCA
  241 TGTTTAATAA ATTACTATAG CAAATTACTT TTTATTCCCA AGGTGTTTAT CTATAATAGT
  301 TAATATTTTA GTCGCTACAT AAAATTTTAC CAAAGAGTAC TTGTATACTA ATTCTAAATG
  361 CCTTCTGACA TAAAACGCCT AGGAAAACAA ACGCAAACAA GGCATCGACT TTTTCAATAA
  421 CCAACCAAAA AAATTTTACA TTAGTCTTTT TTTAATGCTG AGAAAGTCTT TGCTGATATG
  481 CCTTCCAACC AGCTTCTCTA TATCTCTTGG CTTCGACAAC AGGATTACGA CCAGCTCCAT
  541 AGACTCCACG ACCAACAATG ATGATGTCGC TACCGCAGTT TACAATCACT TCTTCAGGAG
  601 TACGATATTG CTGTCCCAGC CCGTCTCCTT TAACATCCAA GCCGATACCA GGGGACATAG
  661 TTATGTAGTC GCTTTGAAGG TTAGGAAATC GACGACCAGC TATAAAGCCA AAGCAAAAAT
  721 CGGTATGCTT CTCAAACCAT TCTAAGGTTT TCTCTGTGTA GGAACCCAGT AGCCAAAGAG
  781 CCCTTTGGGA AGACATTTCA GCCAAAAGCA AGAGACCACG TCCCAAAGTA AACCAACTTC
  841 TTGAGGCCTT GTATAATACC CTCGCCTGCA CTGTATGGCA TTTTGTGATA TGAGCCAGAG
  901 CATTTTTGTA CCACACCAGA TGCATATGTA GCTGACGTAT TCCATGTCTG GCATTTGCGA
  961 TCTCAAGAAA GAAACGATGC TTTTACTTAG GCACAGTTTT CTACAAATTC TGTCGATCTG
1021 AACGCTAATG GCTACATACG ACAGCTGTTC A
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl