RDB06204 - pDUAL2-GFH81

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pDUAL2-GFH81 (#RDB06204)

S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 (low strength) promoter

Clone info.	S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 (low strength) promoter.
Comment	Multicopy marker, ura4+; Integration marker, ura4+, leu1-. Gateway incompatible.
Vector backbone	pDUAL2 (RDB06192) (Plasmid)
Selectable markers	Amp^r
Reference of insert sequence	AB364070.1 (DDBJ accession number)
Depositor\|Developer	Yoshida, Minoru \| Matsuyama, Akihisa \|

Sequence	RDB06204z.seq

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB06204	pDUAL2-GFH81	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDUAL2-GFH81 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06204).

Reference section:

Matsuyama, A., Yoshida, M., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]

Matsuyama, A., Shirai, A., Yashiroda, Y., Kamata, A., Horinouchi, S., Yoshida, M., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14466_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw Sequence file: RDB14466_A6L7a.seq
>06204_14466_A6L7_nmt1Fw_C06_09_ABI24.ab1 1 TGCATTTATT AATCAGGAAA CGTAACTCTC GGCTACTGGA TGGTTCAGTC ACCCAACGAT 61 TACTGGGGAG AGAAAACAGG GCAAAAGCAA AGCTTAAAGG AATCCGATTG TCATTCGGCA 121 ATGTGCAGCG AAACTAAAAA CCGGATAATG GACCTGTTAA TCGAAACATT GAAGATGGAA 181 GAGGAATCCT GGCATATCAT CAATTGAATA AGTTGAATTA ATTATTTCAA TCTCATTCTC 241 ACTTTCTGAC TTATAGTCGC TTTGTTAAAG CTAGCGTCGA CAGGCCTGGA TCCGGATATC 301 ATGAGTAAAG GAGAAGAACT TTTCACTGGA GTTGTCCCAA TTCTTGTTGA ATTAGATGGT 361 GATGTTAATG GGCACAAATT TTCTGTCAGT GGAGAGGGTG AAGGTGATGC AACATACGGA 421 AAACTTACCC TTAAATTTAT TTGCACTACT GGAAAACTAC CTGTTCCGTG GCCAACACTT 481 GTCACTACTT TCTGTTATGG TGTTCAATGC TTTTCAAGAT ACCCAGATCA CATGAAACAG 541 CATGACTTTT TCAAGAGTGC CATGCCCGAA GGCTATGTAC AGGAAAGAAC TATATTTTTC 601 AAAGATGACG GGAACTACAA GACACGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT 661 AATAGAATCG AGTTAAAAGG TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA 721 TTGGAATACA ACTATAACTC ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA 781 ATCAAAGTTA ACTTCAAAAT TAGACACAAC ATTGAAGATG GAAGCGTTCA ACTAGCAGAC 841 CATTATCAAC AAAATACTCC AATTGGCGAT GGCCCTGTCC TTTTACCAGA CAACCATTAC 901 CTGTCCACAC AATCTGCCCT TTCGAAAGAT CCCAACGAAA AGAGAGACCA CATGGGTCCC 961 TTCTTGAGTT GTAACAGCTG CTGGGATTAC ACATGGCATG GATGGACTAT ACAACCCATG 1021 GACCTACAGA ACGAACGATG ACAACATCAC ACATCACCAC TGAGATTCTC GACTCTAGAG 1081 ATCGGCGCGC CACTTCTAAA ATAAAGGCGG A //
Primer: ADH_rev Sequence file: RDB14466_A6L7b.seq
>06204_14466_A6L7_ADH_rev_D06_12_ABI24.ab1 1 GGAGAGGTCT CAGATAGATT TTCGTTTTAA ACCTAAGAGT CACTTTAAAA TTTGTATACA 61 CTTATTTTTT TTATAACTTA TTTAATAATA AAAATCATAA ATCATAAGAA ATTCGCTTAT 121 TTAGAAGTGG CGCGCCGGAT CCTCTAGAGT CGAGATCTCA GTGGTGATGG TGGTGATGTT 181 TGTCATCGTC GTCCTTGTAG TCCATGGGTT TGTATAGTTC ATCCATGCCA TGTGTAATCC 241 CAGCAGCTGT TACAAACTCA AGAAGGACCA TGTGGTCTCT CTTTTCGTTG GGATCTTTCG 301 AAAGGGCAGA TTGTGTGGAC AGGTAATGGT TGTCTGGTAA AAGGACAGGG CCATCGCCAA 361 TTGGAGTATT TTGTTGATAA TGGTCTGCTA GTTGAACGCT TCCATCTTCA ATGTTGTGTC 421 TAATTTTGAA GTTAACTTTG ATTCCATTCT TTTGTTTGTC TGCCATGATG TATACATTGT 481 GTGAGTTATA GTTGTATTCC AATTTGTGTC CAAGAATGTT TCCATCTTCT TTAAAATCAA 541 TACCTTTTAA CTCGATTCTA TTAACAAGGG TATCACCTTC AAACTTGACT TCAGCACGTG 601 TCTTGTAGTT CCCGTCATCT TTGAAAAATA TAGTTCTTTC CTGTACATAG CCTTCGGGCA 661 TGGCACTCTT GAAAAAGTCA TGCTGTTTCA TGTGATCTGG GTATCTTGAA AAGCATTGAA 721 CACCATAACA GAAAGTAGTG ACAAGTGTTG GCCACGGAAC AGGTAGTTTT CCAGTAGTGC 781 AAATAAATTT AAAGGGTAAG TTTTCCGTAT GTTGCATCAC CCTTCACCCT CTCCACTGAC 841 AGAAAATTTG TGCCCATTAA CATCACCATC TAATTTCAAC AAGAATTTGG GACAACTCCA 901 GTGAAAAGTT TCTTCTCCTT TACTCATGAT ATCCCGGGAT CCAGGCCCTG TCGACGCTAG 961 CTTTAACAAA GCGACTATAG TCAGAAAGTG AGAATGGAGA TGAAATTAAT CATCACTAAT 1021 CATGGATGAT ATGCCAAGGA ATCCTCATCA ATCCTCAATG TATCCGAATT ACAGGTTGCT 1081 GA //
Primer: ADH-Forward Sequence file: RDB14466_A6L7c.seq
>06204_14466_A6L7_ADH-Forward_E06_15_ABI24.ab1 1 TGGTCCTTAT TGACACACCT CTACCGGCAG ATCCGCTAGG GATAACAGGG TAATATACTA 61 GTGCCTCTAT TGATGCCTAT GGAACCCCTT TGACTGACGA GACTGTGAAG GCTTGTTTGG 121 AAGCTGACGG TGTTCTTTTG GGTGCCGTTG GTGGTCCTGA ATGGACCAAC CCCAATTGTC 181 GTCCTGAGCA AGGTTTATTG AAGCTTCGTA AGAGTATGGG TGTTTGGGCC AACCTTCGAC 241 CTTGCAACTT TGCCAGCAAG TCTTTAGTCA AGTACAGCCC TTTGAAGCCT GAAATCGTTG 301 AAGGTGTCGA TTTTTGTGTT GTACGAGAAC TTACTGGAGG TTGTTACTTT GGTGAGCGCA 361 CTGAGGACAA CGGATCGGGT TATGCTATGG ACACTTGGCC TTACAGTTTG GAAGAAGTTT 421 CTCGTATTGC TCGTTTGGCG GCCGCGGGCC CAACCTTCCA ATTCATTAAA TCGAGTCCTC 481 CATCAGATGA TTTACAAGGT TTTGCATAGA ATCCCTAAGT AAGAGAAGTT GTTAGTGATT 541 TCTTCAAGTT TACTAAAATT TACATACAAA TGGATGATCT CTTCTCCATT GTTTCCTTTC 601 TTCTTGCAAA CGCGTTTTAC AAAGAGATGA CATGCTATTT TCTGATTATT TTTTTCTATA 661 GTTTTCTATT TTGTACGTCC TTGGGAGTCC GAAATGTAAA ATCGGGGTAT GGTTAGTAGC 721 GGCAGCGTTC ATTATGGTAT ATAAAAGATA AAAAGTAGGG GCAAGCGAGA CAGAATGGGA 781 TACAAAGGGC ATCGTCTATA ATTATAGCTA AAAATTGTAT TTTAATTTGT ATTTTTTGTA 841 ATTTTATTTC TCATTGTTTT ACTTAAAATG AATGCGAAAT TAGAGAAAAC TTCAACGAAA 901 TGTCAAAATA AGCTCAGCAA AATATACAAT TTTTAGGAAA GCGATCAGCA ACCTTATTCT 961 CCGTTGTAGA TTCTTTTTTC TTCATAAATT CGACAATGTA ACTGGCAATG GATTTTTTCT 1021 CTAGATGCTA TTAAATGAAC CTTATCATAT CATAACTCAT GTTCGCATGA TGCTAACTCT 1081 AA //
Primer: f1ori_R3 Sequence file: RDB14466_A6L7d.seq
>06204_14466_A6L7_f1ori_R3_G01_19_ABI24.ab1 1 GCAATGGAGC ATGAGTATGC GGTGTGAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC 61 ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC 121 TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA 181 ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CGAGCTCGGG 241 AGCGCTACCG TGAATGGGCT CAACCAAACA ATGAACCTTT TCTTCTGATT TTCCTACCAC 301 ACCGGAAAGG GAGGCAGAAG GCAAAAGGCC CAAGCTACCA GGAATGACAG AAGCCTCATC 361 TGAAATAATG TCACCAAACA AGTTGTCAGT CAAAACAACA CCGTTAAGTG TACGAGGGCT 421 CTTGACCAAA AGCATGGCTG CGGAGTCAAT GAGCTGGTTT TTTAAGGTAA GGTGAGGATA 481 TTCCTCCTTA AAAATCTTAG CTACAGTCTT GCGCCAAAGA CGAGAAGTTG CCAAAACATT 541 AGCTTTGTCG AGTAATGTGA CGGGAGCAGG AGGGTTGGAA GTTTCAGCTA AGCGGCCGCG 601 GGCCCGAGTC TAACTCCTTA ACCACTCGGA CATAGTGACT TATCTGACAC TCATTGTAAA 661 TTAATATATA TATAGGCATT TTGTTTAGTT AAAGGTACTT AAGTAATTAG TATAAACGAA 721 CCAATTTTAT AATCAGGAAG TTAAGTGAAT GGTAGCACAT GTCGTAAAAA TTGTGAATTT 781 TTATTGAATA ATATTTTAAA TACAAGCCTT TCTAGACTAG GTATACTCAT AAACATATGA 841 GCAAAAGGAT AGAGGAGATT ACATTGCATC TTCTACAAAT TTATTTATTG CCCTTTACTG 901 AAAAATTAAA TAATGAGTAC TAAATGATAA AAAGCGCTCA GTACAAGAAA GATTGCAAAA 961 ATATTGCATT CTTCATGAAA TTAAAGTTGC ATATAAGCAT ATTGAAAGTA ATAGTACTAA 1021 AACAGCAGTT AGCGAAAATT TATAGAATTA TATTCGCAGA CATTGTGACA TTAAATTTAA 1081 AAATGTAGAT TTTTTACTAT CTCATTAACG CATGAGCGTT TATAAAACGC TCAATAAGCT 1141 TTAACCATCC TTTTTTTTTT TGCAGT //
Primer: Reverse2 Sequence file: RDB14466_A6L7e.seq
>06204_14466_A6L7_Reverse2_G06_21_ABI24.ab1 1 CGTAATGCAT TACGCCAGCT TGTGATATTG ACGAAACTTT TTGACATCTA ATTTATTCTG 61 TTCCAACACC AATGTTTATA ACCAAGTTTT ATCTTGTTTG TCTACATGGT ATTTTACATT 121 CATCTACATA CATCTTTCAT TGGCTTTGTA CATAGTTATC ATTACAAGTC TAAAAAAATT 181 CACTCTTTTC TTATTCAATG TCAATCCAAG AGAAAAGATT GTGGTAATGT TGTAGGAGCA 241 TGTTTAATAA ATTACTATAG CAAATTACTT TTTATTCCCA AGGTGTTTAT CTATAATAGT 301 TAATATTTTA GTCGCTACAT AAAATTTTAC CAAAGAGTAC TTGTATACTA ATTCTAAATG 361 CCTTCTGACA TAAAACGCCT AGGAAAACAA ACGCAAACAA GGCATCGACT TTTTCAATAA 421 CCAACCAAAA AAATTTTACA TTAGTCTTTT TTTAATGCTG AGAAAGTCTT TGCTGATATG 481 CCTTCCAACC AGCTTCTCTA TATCTCTTGG CTTCGACAAC AGGATTACGA CCAGCTCCAT 541 AGACTCCACG ACCAACAATG ATGATGTCGC TACCGCAGTT TACAATCACT TCTTCAGGAG 601 TACGATATTG CTGTCCCAGC CCGTCTCCTT TAACATCCAA GCCGATACCA GGGGACATAG 661 TTATGTAGTC GCTTTGAAGG TTAGGAAATC GACGACCAGC TATAAAGCCA AAGCAAAAAT 721 CGGTATGCTT CTCAAACCAT TCTAAGGTTT TCTCTGTGTA GGAACCCAGT AGCCAAAGAG 781 CCCTTTGGGA AGACATTTCA GCCAAAAGCA AGAGACCACG TCCCAAAGTA AACCAACTTC 841 TTGAGGCCTT GTATAATACC CTCGCCTGCA CTGTATGGCA TTTTGTGATA TGAGCCAGAG 901 CATTTTTGTA CCACACCAGA TGCATATGTA GCTGACGTAT TCCATGTCTG GCATTTGCGA 961 TCTCAAGAAA GAAACGATGC TTTTACTTAG GCACAGTTTT CTACAAATTC TGTCGATCTG 1021 AACGCTAATG GCTACATACG ACAGCTGTTC A //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]
original	Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]

Resource search

Link

Other resources in our bank

External Database

Reference sequence