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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDUAL2-GFH81

S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 (low strength) promoter

Catalog number RDB06204
Resource name pDUAL2-GFH81
Clone info. S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 (low strength) promoter.
Comment Multicopy marker, ura4+; Integration marker, ura4+, leu1-. Gateway incompatible.
Vector backbone pDUAL2 (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364070.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06204 pDUAL2-GFH81 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06204z.seq

            Original reference

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14466_A6L7p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB14466_A6L7a.seq check
            >06204_14466_A6L7_nmt1Fw_C06_09_ABI24.ab1
                1 TGCATTTATT AATCAGGAAA CGTAACTCTC GGCTACTGGA TGGTTCAGTC ACCCAACGAT
               61 TACTGGGGAG AGAAAACAGG GCAAAAGCAA AGCTTAAAGG AATCCGATTG TCATTCGGCA
              121 ATGTGCAGCG AAACTAAAAA CCGGATAATG GACCTGTTAA TCGAAACATT GAAGATGGAA
              181 GAGGAATCCT GGCATATCAT CAATTGAATA AGTTGAATTA ATTATTTCAA TCTCATTCTC
              241 ACTTTCTGAC TTATAGTCGC TTTGTTAAAG CTAGCGTCGA CAGGCCTGGA TCCGGATATC
              301 ATGAGTAAAG GAGAAGAACT TTTCACTGGA GTTGTCCCAA TTCTTGTTGA ATTAGATGGT
              361 GATGTTAATG GGCACAAATT TTCTGTCAGT GGAGAGGGTG AAGGTGATGC AACATACGGA
              421 AAACTTACCC TTAAATTTAT TTGCACTACT GGAAAACTAC CTGTTCCGTG GCCAACACTT
              481 GTCACTACTT TCTGTTATGG TGTTCAATGC TTTTCAAGAT ACCCAGATCA CATGAAACAG
              541 CATGACTTTT TCAAGAGTGC CATGCCCGAA GGCTATGTAC AGGAAAGAAC TATATTTTTC
              601 AAAGATGACG GGAACTACAA GACACGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT
              661 AATAGAATCG AGTTAAAAGG TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA
              721 TTGGAATACA ACTATAACTC ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA
              781 ATCAAAGTTA ACTTCAAAAT TAGACACAAC ATTGAAGATG GAAGCGTTCA ACTAGCAGAC
              841 CATTATCAAC AAAATACTCC AATTGGCGAT GGCCCTGTCC TTTTACCAGA CAACCATTAC
              901 CTGTCCACAC AATCTGCCCT TTCGAAAGAT CCCAACGAAA AGAGAGACCA CATGGGTCCC
              961 TTCTTGAGTT GTAACAGCTG CTGGGATTAC ACATGGCATG GATGGACTAT ACAACCCATG
             1021 GACCTACAGA ACGAACGATG ACAACATCAC ACATCACCAC TGAGATTCTC GACTCTAGAG
            1081 ATCGGCGCGC CACTTCTAAA ATAAAGGCGG A
            //
            Primer: ADH_rev
            Sequence file: RDB14466_A6L7b.seq check
            >06204_14466_A6L7_ADH_rev_D06_12_ABI24.ab1
                1 GGAGAGGTCT CAGATAGATT TTCGTTTTAA ACCTAAGAGT CACTTTAAAA TTTGTATACA
               61 CTTATTTTTT TTATAACTTA TTTAATAATA AAAATCATAA ATCATAAGAA ATTCGCTTAT
              121 TTAGAAGTGG CGCGCCGGAT CCTCTAGAGT CGAGATCTCA GTGGTGATGG TGGTGATGTT
              181 TGTCATCGTC GTCCTTGTAG TCCATGGGTT TGTATAGTTC ATCCATGCCA TGTGTAATCC
              241 CAGCAGCTGT TACAAACTCA AGAAGGACCA TGTGGTCTCT CTTTTCGTTG GGATCTTTCG
              301 AAAGGGCAGA TTGTGTGGAC AGGTAATGGT TGTCTGGTAA AAGGACAGGG CCATCGCCAA
              361 TTGGAGTATT TTGTTGATAA TGGTCTGCTA GTTGAACGCT TCCATCTTCA ATGTTGTGTC
              421 TAATTTTGAA GTTAACTTTG ATTCCATTCT TTTGTTTGTC TGCCATGATG TATACATTGT
              481 GTGAGTTATA GTTGTATTCC AATTTGTGTC CAAGAATGTT TCCATCTTCT TTAAAATCAA
              541 TACCTTTTAA CTCGATTCTA TTAACAAGGG TATCACCTTC AAACTTGACT TCAGCACGTG
              601 TCTTGTAGTT CCCGTCATCT TTGAAAAATA TAGTTCTTTC CTGTACATAG CCTTCGGGCA
              661 TGGCACTCTT GAAAAAGTCA TGCTGTTTCA TGTGATCTGG GTATCTTGAA AAGCATTGAA
              721 CACCATAACA GAAAGTAGTG ACAAGTGTTG GCCACGGAAC AGGTAGTTTT CCAGTAGTGC
              781 AAATAAATTT AAAGGGTAAG TTTTCCGTAT GTTGCATCAC CCTTCACCCT CTCCACTGAC
              841 AGAAAATTTG TGCCCATTAA CATCACCATC TAATTTCAAC AAGAATTTGG GACAACTCCA
              901 GTGAAAAGTT TCTTCTCCTT TACTCATGAT ATCCCGGGAT CCAGGCCCTG TCGACGCTAG
              961 CTTTAACAAA GCGACTATAG TCAGAAAGTG AGAATGGAGA TGAAATTAAT CATCACTAAT
             1021 CATGGATGAT ATGCCAAGGA ATCCTCATCA ATCCTCAATG TATCCGAATT ACAGGTTGCT
            1081 GA
            //
            Primer: ADH-Forward
            Sequence file: RDB14466_A6L7c.seq check
            >06204_14466_A6L7_ADH-Forward_E06_15_ABI24.ab1
                1 TGGTCCTTAT TGACACACCT CTACCGGCAG ATCCGCTAGG GATAACAGGG TAATATACTA
               61 GTGCCTCTAT TGATGCCTAT GGAACCCCTT TGACTGACGA GACTGTGAAG GCTTGTTTGG
              121 AAGCTGACGG TGTTCTTTTG GGTGCCGTTG GTGGTCCTGA ATGGACCAAC CCCAATTGTC
              181 GTCCTGAGCA AGGTTTATTG AAGCTTCGTA AGAGTATGGG TGTTTGGGCC AACCTTCGAC
              241 CTTGCAACTT TGCCAGCAAG TCTTTAGTCA AGTACAGCCC TTTGAAGCCT GAAATCGTTG
              301 AAGGTGTCGA TTTTTGTGTT GTACGAGAAC TTACTGGAGG TTGTTACTTT GGTGAGCGCA
              361 CTGAGGACAA CGGATCGGGT TATGCTATGG ACACTTGGCC TTACAGTTTG GAAGAAGTTT
              421 CTCGTATTGC TCGTTTGGCG GCCGCGGGCC CAACCTTCCA ATTCATTAAA TCGAGTCCTC
              481 CATCAGATGA TTTACAAGGT TTTGCATAGA ATCCCTAAGT AAGAGAAGTT GTTAGTGATT
              541 TCTTCAAGTT TACTAAAATT TACATACAAA TGGATGATCT CTTCTCCATT GTTTCCTTTC
              601 TTCTTGCAAA CGCGTTTTAC AAAGAGATGA CATGCTATTT TCTGATTATT TTTTTCTATA
              661 GTTTTCTATT TTGTACGTCC TTGGGAGTCC GAAATGTAAA ATCGGGGTAT GGTTAGTAGC
              721 GGCAGCGTTC ATTATGGTAT ATAAAAGATA AAAAGTAGGG GCAAGCGAGA CAGAATGGGA
              781 TACAAAGGGC ATCGTCTATA ATTATAGCTA AAAATTGTAT TTTAATTTGT ATTTTTTGTA
              841 ATTTTATTTC TCATTGTTTT ACTTAAAATG AATGCGAAAT TAGAGAAAAC TTCAACGAAA
              901 TGTCAAAATA AGCTCAGCAA AATATACAAT TTTTAGGAAA GCGATCAGCA ACCTTATTCT
              961 CCGTTGTAGA TTCTTTTTTC TTCATAAATT CGACAATGTA ACTGGCAATG GATTTTTTCT
             1021 CTAGATGCTA TTAAATGAAC CTTATCATAT CATAACTCAT GTTCGCATGA TGCTAACTCT
            1081 AA
            //
            Primer: f1ori_R3
            Sequence file: RDB14466_A6L7d.seq check
            >06204_14466_A6L7_f1ori_R3_G01_19_ABI24.ab1
                1 GCAATGGAGC ATGAGTATGC GGTGTGAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC
               61 ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC
              121 TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA
              181 ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CGAGCTCGGG
              241 AGCGCTACCG TGAATGGGCT CAACCAAACA ATGAACCTTT TCTTCTGATT TTCCTACCAC
              301 ACCGGAAAGG GAGGCAGAAG GCAAAAGGCC CAAGCTACCA GGAATGACAG AAGCCTCATC
              361 TGAAATAATG TCACCAAACA AGTTGTCAGT CAAAACAACA CCGTTAAGTG TACGAGGGCT
              421 CTTGACCAAA AGCATGGCTG CGGAGTCAAT GAGCTGGTTT TTTAAGGTAA GGTGAGGATA
              481 TTCCTCCTTA AAAATCTTAG CTACAGTCTT GCGCCAAAGA CGAGAAGTTG CCAAAACATT
              541 AGCTTTGTCG AGTAATGTGA CGGGAGCAGG AGGGTTGGAA GTTTCAGCTA AGCGGCCGCG
              601 GGCCCGAGTC TAACTCCTTA ACCACTCGGA CATAGTGACT TATCTGACAC TCATTGTAAA
              661 TTAATATATA TATAGGCATT TTGTTTAGTT AAAGGTACTT AAGTAATTAG TATAAACGAA
              721 CCAATTTTAT AATCAGGAAG TTAAGTGAAT GGTAGCACAT GTCGTAAAAA TTGTGAATTT
              781 TTATTGAATA ATATTTTAAA TACAAGCCTT TCTAGACTAG GTATACTCAT AAACATATGA
              841 GCAAAAGGAT AGAGGAGATT ACATTGCATC TTCTACAAAT TTATTTATTG CCCTTTACTG
              901 AAAAATTAAA TAATGAGTAC TAAATGATAA AAAGCGCTCA GTACAAGAAA GATTGCAAAA
              961 ATATTGCATT CTTCATGAAA TTAAAGTTGC ATATAAGCAT ATTGAAAGTA ATAGTACTAA
             1021 AACAGCAGTT AGCGAAAATT TATAGAATTA TATTCGCAGA CATTGTGACA TTAAATTTAA
             1081 AAATGTAGAT TTTTTACTAT CTCATTAACG CATGAGCGTT TATAAAACGC TCAATAAGCT
            1141 TTAACCATCC TTTTTTTTTT TGCAGT
            //
            Primer: Reverse2
            Sequence file: RDB14466_A6L7e.seq check
            >06204_14466_A6L7_Reverse2_G06_21_ABI24.ab1
                1 CGTAATGCAT TACGCCAGCT TGTGATATTG ACGAAACTTT TTGACATCTA ATTTATTCTG
               61 TTCCAACACC AATGTTTATA ACCAAGTTTT ATCTTGTTTG TCTACATGGT ATTTTACATT
              121 CATCTACATA CATCTTTCAT TGGCTTTGTA CATAGTTATC ATTACAAGTC TAAAAAAATT
              181 CACTCTTTTC TTATTCAATG TCAATCCAAG AGAAAAGATT GTGGTAATGT TGTAGGAGCA
              241 TGTTTAATAA ATTACTATAG CAAATTACTT TTTATTCCCA AGGTGTTTAT CTATAATAGT
              301 TAATATTTTA GTCGCTACAT AAAATTTTAC CAAAGAGTAC TTGTATACTA ATTCTAAATG
              361 CCTTCTGACA TAAAACGCCT AGGAAAACAA ACGCAAACAA GGCATCGACT TTTTCAATAA
              421 CCAACCAAAA AAATTTTACA TTAGTCTTTT TTTAATGCTG AGAAAGTCTT TGCTGATATG
              481 CCTTCCAACC AGCTTCTCTA TATCTCTTGG CTTCGACAAC AGGATTACGA CCAGCTCCAT
              541 AGACTCCACG ACCAACAATG ATGATGTCGC TACCGCAGTT TACAATCACT TCTTCAGGAG
              601 TACGATATTG CTGTCCCAGC CCGTCTCCTT TAACATCCAA GCCGATACCA GGGGACATAG
              661 TTATGTAGTC GCTTTGAAGG TTAGGAAATC GACGACCAGC TATAAAGCCA AAGCAAAAAT
              721 CGGTATGCTT CTCAAACCAT TCTAAGGTTT TCTCTGTGTA GGAACCCAGT AGCCAAAGAG
              781 CCCTTTGGGA AGACATTTCA GCCAAAAGCA AGAGACCACG TCCCAAAGTA AACCAACTTC
              841 TTGAGGCCTT GTATAATACC CTCGCCTGCA CTGTATGGCA TTTTGTGATA TGAGCCAGAG
              901 CATTTTTGTA CCACACCAGA TGCATATGTA GCTGACGTAT TCCATGTCTG GCATTTGCGA
              961 TCTCAAGAAA GAAACGATGC TTTTACTTAG GCACAGTTTT CTACAAATTC TGTCGATCTG
            1021 AACGCTAATG GCTACATACG ACAGCTGTTC A
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            2022.07.13

            GNP_filter3_RDBDEP_html_220713.pl