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pRSFlag_mmSumo1

Expression vector of mouse Sumo1 coding sequence

Catalog number RDB06126
Resource name pRSFlag_mmSumo1
Alternative name SET-0156_4
Clone info. Expression vector of mouse Sumo1 coding sequence. Expression was confirmed by Western blotting with anti-FLAG antibody.
Vector backbone pRSV_s-FLAG (Plasmid)
Size of vector backbone 5.19 kb
Selectable markers Amp^r
Gene/insert name Mouse Sumo1 cDNA
Depositor|Developer DNA Bank, | Murata, Takehide |
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External Database
Mouse Sumo1

          Reference sequence
            

          Distribution information

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          RDB06126 pRSFlag_mmSumo1 DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB06126z.seq

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content pCMV_tag and pRSV_tag assay-ready clones (English text)
          Featured content Dnaconda's recommendation EXR0005j (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13461_A5G7.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: RSVpro_F
          Sequence file: RDB13461_A5G7a.seq check
          >06126_13461_A5G7_RSVpro_F_A10_01.ab1
              1 AAGTTTGACA TTCACCACAT TGGTGTGCAC CTCCAAGCTG AATTCACCAT GGACTACAAG
             61 GACGATGACG ATAAGGCGGC CATGTCTGAC CAGGAGGCAA AACCTTCAAC TGAGGACTTA
            121 GGCGATAAGA AGGAAGGAGA ATACATTAAA CTCAAAGTTA TTGGACAGGA TAGCAGTGAG
            181 ATACATTTCA AAGTGAAAAT GACAACACAT CTCAAGAAAC TCAAAGAATC ATACTGTCAA
            241 AGACAGGGAG TTCCAATGAA TTCACTCAGG TTTCTCTTTG AAGGTCAGAG AATTGCTGAT
            301 AATCATACTC CGAAAGAACT GGGAATGGAG GAAGAAGATG TGATTGAAGT TTATCAGGAA
            361 CAAACGGGGG GTCACTCGAC GGTTTAGGGC CGCTCGAGCA TGCATCTAGA GCTCGCTGAT
            421 CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT
            481 CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT
            541 CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG
            601 GGGAGGGTTG GGAAGACAAT AGCAGGCATG CTGGGGATGC GGTGGGCTCT ATGGCTTCTG
            661 AGGCGGAAAG AACCAGCTGG GGCTCGAGGG GGGATCCCCA CGCGCCCTGT AGCGGCGCAT
            721 TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG
            781 CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGGT
            841 CAAGCTCTAA ATCGGGGGGC TCCCCTTTAG GGGTTCCGAT TTTAGTGCTT TAACGGCACC
            901 TCGACCCCAA AAAACTTGAT TAGGGGTGAT GGTTTCACGT TATTGGGCCA TCGCCCCTGA
          961 TAAACGGTTT TTTCACCCTT TGACGTGGAA GTTCCACCGT TCTTTTAAAA ATGGA
          //
          Primer: SV40pro_F_V2
          Sequence file: RDB13461_A5G7b.seq check
          >06126_13461_A5G7_SV40proF_V2_B10_04.ab1
              1 TGGCCCGTTT TTGGAGCCTA GGCTTTTGCA AAAGCTCCCG GGAGCTTGGA TATCCATTTT
             61 CGGATCTGAT CAAGAGGCAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA
            121 CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC
            181 AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT
            241 TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC
            301 GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG
            361 AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC
            421 TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC
            481 GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT
            541 GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC
            601 CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA
            661 TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA
            721 CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT
            781 TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC
            841 TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT
            901 CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC
            961 ACCGCCGCCT TCTATGAAAG GTTGGGGCTT CGGAATCGTT TTCCGGGACG CCCGGCTGGA
           1021 ATGATCCTTC CAGCGCGGGG ATCTCATGCT GGAGTTTCTT CGCCCACCCC AACTTTGTTA
           1081 TTGCAGCCTT ATATGGGTTA ACAAATTAAA GCATAGGCAA TTCACCAAAA ATTTTTCCAC
          1141 C
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          user report Gur, I., Necdin promotes ubiquitin-dependent degradation of PIAS1 SUMO E3 ligase. PLoS One, 9 (6): e99503 (2014). PMID 24911587.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl