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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCMFlag_hsRXR alpha (#RDB06112)

Expression vector of human RXR alpha.

Alternative name SET-0094_1
Clone info. Expression vector of human RXR alpha coding sequence. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment PCR cloning, forward primer: gacaccaaacatttcctgccgct; reverse primer: ctaagtcatttggtgcggcgcct.
Vector backbone pCMV_S-FLAG (RDB05956) (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human RXR alpha cDNA
Depositor|Developer DNA Bank, |
 
Sequence RDB06112z.seq
Remarks, protocol and/or map (pdf) RDB06112.pdf

Distribution information

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RDB06112 pCMFlag_hsRXR alpha DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMFlag_hsRXR alpha was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06112).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18388_B0Hqp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,FLAG,insert 5'
Sequence file: RDB18388_B0Hqa.seq check
>06112_18388_B0Hq_3_CMV_Forward_A11_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNTGNN GCNNNGGGCG GTAGGCGTGT ACGGTGGGNN GTCTATATAA
   61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
  121 CTCACTATAG GGAGACCCAA GCTGAATTCA CCATGGACTA CAAGGACGAT GACGATAAGG
  181 CGGCCGACAC CAAACATTTC CTGCCGCTCG ATTTCTCCAC CCAGGTGAAC TCCTCCCTCA
  241 CCTCCCCGAC GGGGCGAGGC TCCATGGCTG CCCCCTCGCT GCACCCGTCC CTGGGGCCTG
  301 GCATCGGCTC CCCGGGACAG CTGCATTCTC CCATCAGCAC CCTGAGCTCC CCCATCAACG
  361 GCATGGGCCC GCCTTTCTCG GTCATCAGCT CCCCCATGGG CCCCCACTCC ATGTCGGTGC
  421 CCACCACACC CACCCTGGGC TTCAGCACTG GCAGCCCCCA GCTCAGCTCA CCTATGAACC
  481 CCGTCAGCAG CAGCGAGGAC ATCAAGCCCC CCCTGGGCCT CAATGGCGTC CTCAAGGTCC
  541 CCGCCCACCC CTCAGGAAAC ATGGCTTCCT TCACCAAGCA CATCTGCGCC ATCTGCGGGG
  601 ACCGCTCCTC AGGCAAGCAC TATGGAGTGT ACAGCTGCGA GGGGTGCAAG GGCTTCTTCA
  661 AGCGGACGGT GCGCAAGGAC CTGACCTACA CCTGCCGCGA CAACAAGGAC TGCCTGATTG
  721 ACAAGCGGCA GCGGAACCGG TGCCAGTACT GCCGCTACCA GAAGTGCCTG GCCATGGGCA
  781 TGAAGCGGGA AGCCGTGCAN GANGANCGGC AGCGTGGCAA GGANCGGANC GAGAATGANG
  841 TGGANTCGAN CAGCAGCGCC NACGANGANA TGCCGGNGGA NANGNNNCTG NANGCTGANC
  901 TGGCCGNGGA NCCCNAGANC NANANNNACN TGGNNGNNAA NATGGGGGNT NNNNCNNANC
961 NCNNNNNNNN ANCNNNNNNN NNN
//
Primer: bGH_rev3 (Pr0885)
Region: bGHpA,insert 3'
Sequence file: RDB18388_B0Hqb.seq check
>06112_18388_B0Hq_3_BGH_rev3_B11_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNGNN GNNGGGNANN NACAGANGGC TGGCAACTAG AAGGCACAGT
   61 CGAGGCTGAT CAGCGAGCTC TAGCATTTAG GTGACACTAT AGAATAGGGC CCTCTAGATG
  121 CATGCTCGAG CGGCCCTAAG TCATTTGGTG CGGCGCCTCC AGCATCTCCA TAAGGAAGGT
  181 GTCAATGGGT GTGTCCCCGA TGAGCTTGAA GAAGAAGAGA TGTTCCAGGC ATTTGAGCCC
  241 GATGGAGCGC AGAGCCGGCA GGCGGAGCAA GAGCTTAGCG AACCTTCCCG GCTGCTCTGG
  301 GTACTTGTGC TTGCAGTAGG CCTCCAAGGA CGCATAGACC TTCTCCCTCA GCGCCTCCAC
  361 CTCGGCCGGG TTCGAGAGCC CCTTGGAGTC AGGGTTAAAG AGGACGATGG CGCGCAGGCA
  421 GCCCAGCTCC GTCTTGTCCA TCTGCATGTC CCGCATCTTG GACACAAGCT CCGTCAGCAC
  481 CCTGTCAAAG ATGGCGCCCA CCCCTGCGCT GTGGGCGCTG TTCCGGTGGA CGTGCAGCCC
  541 GGTGGCCAGG AGGATCCCGT CCTTCACGGC GATGGAGCGG TGGGAGAAGG AGGCGATGAG
  601 CAGCTCATTC CAGCCTGCCC GCAGCAGGAT GACCTGGTCG TCCAGGGGCA GCTCTGAGAA
  661 GTGTGGGATC CGCTTGGCCC ACTCCACCAG GGTGAAAAGC TGTTTGTCGG CTGCTTGGCA
  721 AATGTTGGTG ACAGGGTCGT TCGGCGAGCT GGGGTTCAGC CCCATGTTTG CCTCCACGTA
  781 NGTCTCGGTC TTGGGCTCCA CGGCCAGCTC AGCCTCCAGG ANCCTCTCCA CCGGCATGTC
  841 CTCGNTGGCG CTGCTGGNCG ACTCCACNNC ATTCTCGTNN CGGNCCTTGC CACGCTGCCG
  901 CTCCTCCTGC ANGGCTTCCC GCTTCATGCC CANNGCCAGG CNNTTCNNGT ANCGGCNNTA
961 NNNNNACCNN NNNNNCNNNN GNTNNNNNNN NNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori,NeoR
Sequence file: RDB18388_B0Hqc.seq check
>06112_18388_B0Hq_3_SV40pro_ori_F_C11_03_ABI08.ab1
    1 NNNNNNNNNN NNNNCGCCNC NNCCTCTGNN CTANTCNNGA AGTAGTGAGG AGGCTTTTTT
   61 GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGGA TATCCATTTT CGGATCTGAT
  121 CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT
  181 CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC
  241 TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC
  301 GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC
  361 ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG
  421 CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG
  481 AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC
  541 CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT
  601 CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC
  661 GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GANGATCTCG TCGTGACCCA TGGCGATGCC
  721 TGCTTGCCGA ATATCATGGT GGANNATGNC CGCTTTTCTG GATTCATCGA CTGTGGCCGG
  781 CTGGGTGTGG CGGANCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAN
  841 CTTGNCGGCG AATGGGCTGA CCGCTTCCTC NNGCTTTACN GNATCGCCGC NNCNNANTCN
  901 CAGCGCATCG CCTTCTANCG CCTTCTNGNC NANTTCNTCN NANCGGNNNN NNGGGNNNCN
961 NAATGNNCNA NNNNGCGNNN NCNNNNCNNN NCNNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles