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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CS-RfA-EVBsd

Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with Venus and Bsd marker driven by EF-1 alpha promoter.

Catalog number RDB06090
Resource name CS-RfA-EVBsd
Clone info. Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with Venus and Bsd marker driven by EF-1 alpha promoter.
Commonly requested with pENTR4-H1 (RDB04395) for generation of shRNA expression vector and with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394) for packaging. Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone Plasmid
Selectable markers Lentivirus transfection: Bsd^r. E. coli: Amp^r, Cm^r, ccdB. Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Miyoshi, Hiroyuki |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding Venus fluorescent protein.
Ordering Please visit Ordering instruction.[link] 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Please obtain written approval of Dr. Atsushi Miyawaki.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06090 CS-RfA-EVBsd DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB06090z.seq
Electronic file Remarks, protocol and/or map (pdf) RDB06090.pdf

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18387_B0Hop1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: Cm_M_R
Region: CmR, attR1, cPPT_CTS
Sequence file: RDB18387_B0Hoa.seq check
>06090_18387_B0Ho_1B_Cm_M_R_A10_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NATNNCNNGC TNNCCGTCTT TNNTTGCCNT ACGGAATTCC
   61 GGATGAGCAT TCATCAGGCG GGCAAGAATG TGAATAAAGG CCGGATAAAA CTTGTGCTTA
  121 TTTTTCTTTA CGGTCTTTAA AAAGGCCGTA ATATCCAGCT GAACGGTCTG GTTATAGGTA
  181 CATTGAGCAA CTGACTGAAA TGCCTCAAAA TGTTCTTTAC GATGCCATTG GGATATATCA
  241 ACGGTGGTAT ATCCAGTGAT TTTTTTCTCC ATTTTAGCTT CCTTAGCTCC TGAAAATCTC
  301 GACGGATCCT AACTCAAAAT CCACACATTA TACGAGCCGG AAGCATAAAG TGTAAAGCCT
  361 GGGGTGCCTA ATGCGGCCGC CATAGTGACT GGATATGTTG TGTTTTACAG TATTATGTAG
  421 TCTGTTTTTT ATGCAAAATC TAATTTAATA TATTGATATT TATATCATTT TACGTTTCTC
  481 GTTCAGCTTT TTTGTACAAA CTTGTGATAA TTCAAAGTGA ATTTCTGCTG TCCCTGTAAT
  541 AAACCCGAAA ATTTTGAATT TTTGTAATTT GTTTTTGTAA TTCTTTAGTT TGTATGTCTG
  601 TTGCTATTAT GTCTACTATT CTTTCCCCTG CACTGTACCC CCCAATCCCC CCTTTTCTTT
  661 TAAAATTGTG GATGAATACT GCCATTTGTA GATCCGTTCA CTAATCGAAT GGATCTGTCT
  721 CTGTCTCTCT CTCCACCTTC TTCTTCTATT CCTTCGGGCC TGTCGGGTCC CCTCGGGGTT
  781 NGGANGTGGG TCTGAAACGA TAATGGTGAA TATCCCTGCC TAACTCTATT CACTATAGAA
  841 AGTACAGCAA AAACTATTCT TAAACNNACC AAGCCTNCTA CTATCATTAT GAATAATNNN
  901 ATATANNNCA GCCNATTTNG TTATGNTAAA NNNATTNNNC NAACTTNCCC ATTTATCNNA
961 NTNNNNNAAN TNNNGNTNNN NNNTTTNNNN NNNNNN
//
Primer: EF1a-N_R
Region: EF-1alpha pro, attR2, ccdB
Sequence file: RDB18387_B0Hob.seq check
>06090_18387_B0Ho_1B_EF1a-N_R_B10_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGG CGNNGTGCGC TCTGCCCNCT NNCGGGCACC GGANCCTCAC
   61 GAAGCTTGAA TTATCACCAC TTTGTACAAG AAAGCTGAAC GAGAAACGTA AAATGATATA
  121 AATATCAATA TATTAAATTA GATTTTGCAT AAAAAACAGA CTACATAATA CTGTAAAACA
  181 CAACATATCC AGTCACTATG GTCGACCTGC AGACTGGCTG TGTATAAGGG AGCCTGACAT
  241 TTATATTCCC CAGAACATCA GGTTAATGGC GTTTTTGATG TCATTTTCGC GGTGGCTGAG
  301 ATCAGCCACT TCTTCCCCGA TAACGGAGAC CGGCACACTG GCCATATCGG TGGTCATCAT
  361 GCGCCAGCTT TCATCCCCGA TATGCACCAC CGGGTAAAGT TCACGGGAGA CTTTATCTGA
  421 CAGCAGACGT GCACTGGCCA GGGGGATCAC CATCCGTCGC CCGGGCGTGT CAATAATATC
  481 ACTCTGTACA TCCACAAACA GACGATAACG GCTCTCTCTT TTATAGGTGT AAACCTTAAA
  541 CTGCATTTCA CCAGCCCCTG TTCTCGTCAG CAAAAGAGCC GTTCATTTCA ATAAACCGGG
  601 CGACCTCAGC CATCCCTTCC TGATTTTCCG CTTTCCAGCG TTCGGCACGC AGACGACGGG
  661 CTTCATTCTG CATGGTTGTG CTTACCAGAC CGGAGATATT GACATCATAT ATGCCTTGAG
  721 CAACTGATAG CTGTCGCTGT CAACTGTCAC TGTAATACGC TGCTTCATAN CATACCTCTT
  781 TTTGACATAC TTCGGGTATA CATATCAGTA TATATTCTTA TACCGCAAAA ATCAGCGCGC
  841 AAATACGCAT ACTGTTATCT GGCTTTTANN AAGCCGGATN CACNCNTTTA CGCCCCNCCC
  901 NGCCNNTCAT CNCANNANTG NNGNNANTNA TTNAGCATTN NGNNNNNNTG GNANNCNNNN
961 NNNANNNNNN NNNN
//
Primer: EF1a-C_F
Region: EF-1alpha pro, Venus 5'
Sequence file: RDB18387_B0Hoc.seq check
>06090_18387_B0Ho_1B_EF1a-C_F_A01_01_ABI24.ab1
    1 NNNNNNNNNN NNTTTTGANT TNGGNNCTTG GTTNNTTCTC NAGCCTCAGA CAGTGGTTCA
   61 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AATTAATTAA CCGGTTCCAC AACCATGGTG
  121 AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC
  181 GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG
  241 CTGACCCTGA AGCTGATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG
  301 ACCACCCTGG GCTACGGCCT GCAGTGCTTC GCCCGCTACC CCGACCACAT GAAGCAGCAC
  361 GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG
  421 GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC
  481 CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG
  541 GAGTACAACT ACAACAGCCA CAACGTCTAT ATCACCGCCG ACAAGCAGAA GAACGGCATC
  601 AAGGCCAACT TCAAGATCCG CCACAACATC GAGGACGGCG GCGTGCAGCT CGCCGACCAC
  661 TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG
  721 AGCTACCAGT CCGCCCTGAN CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG
  781 GNNTTCGTGA CCGCCGCCGG NNTCACTCTC GGCATGGNCG ANCTGTACAA NGGNTCCGGC
  841 NAGGGCANAN GAAGTCTTCT AACATGCNGN GANGNNGNNG AAAATNCNGG GCCCNNNGNN
901 NANCNTTNNN CTCAANAANN NNCCNNCNNN ATNNAAAGAN CNNNNGNNNN NNNNNN
//
Primer: PRE_rev
Region: PRE,BSD,T2A,Venus 3'
Sequence file: RDB18387_B0Hoh.seq check
>06090_18387_B1ArF_PRE_rev_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGNN NNNNNNANNT NANAATANNN NNCAANTCTT
   61 TCNCAAATTT TGTAATCCAG AGGTTGATTA TCGATAAGCT TGATCCCTCG AGTGCGGCCT
  121 TAGCCCTCCC ACACATAACC AGAGGGCAGC AATTCACGAA TCCCAACTGC CGTCGGCTGT
  181 CCATCACTGT CCTTCACTAT GGCTTTGATC CCAGGATGCA GATCGAGAAG CACCTGTCGG
  241 CACCGTCCGC AGGGGCTCAA GATGCCCCTG TTCTCATTTC CGATCGCGAC GATACAAGTC
  301 AGGTTGCCAG CTGCCGCAGC AGCAGCAGTG CCCAGCACCA CGAGTTCTGC ACAAGGTCCC
  361 CCAGTAAAAT GATATACATT GACACCAGTG AAGATGCGGC CGTCGCTAGA GAGAGCTGCG
  421 CTGGCGACGC TGTAGTCTTC AGAGATGGGG ATGCTGTTGA TTGTAGCCGT TGCTCTTTCA
  481 ATGAGGGTGG ATTCTTCTTG AGACAAAGGC TTGGCCATGG GCCCGGGATT TTCCTCCACG
  541 TCACCGCATG TTAGAAGACT TCCTCTGCCC TCGCCGGATC CCTTGTACAG CTCGTCCATG
  601 CCGAGAGTGA TCCCGGCGGC GGTCACGANC TCCNGCAGGA CCATGTGATC GCGCTTCTCG
  661 TTGGGGTCTT TGCTCNNNNN GANNNGNANC TCNNNANNGN NNGNCNGGNN NCANCACGGG
721 NNNNCNCNNN GGGGNNNNNG CNNNNNNNNN N
//
Primer: CMV_Forward
Region: CMV pro,5' LTR,HIV-1 psi
Sequence file: RDB18387_B0Hod.seq check
>06090_18387_B0Ho_1B_CMV_Forward_D10_10_ABI24.ab1
    1 NNNNNNNNNN NNNNTTNNNG CNNTGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGCGCGTTT TGCCTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT
  121 CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG
  181 TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT
  241 CAGTGTGGAA AATCTCTAGC AGTGGCGCCC GAACAGGGAC TTGAAAGCGA AAGGGAAACC
  301 AGAGGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG
  361 GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG
  421 GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT
  481 AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT
  541 AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT
  601 GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC
  661 AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT
  721 AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCGCTGA
  781 TCTTCAGACC TGGNNGANGA NATATGANGG ANAATTGGAG AAGTGAATTA TATAAATATN
  841 AAGTAGTAAA AAATTGAANC NTTNNGANTA GCACCCNCCN NGGNNAAGAN AANANNNGNG
  901 CANNNNNAAA AAAAGANCAN NGGNNANTAN GNNNCTTTNN NNNNNNGGNN TNNNNNGNNN
961 NNNCNNNANN NNNNNNNGNN NNNNNNNN
//
Primer: BSD_R1
Region: BSD,T2A,Venus 3'
Sequence file: RDB18387_B0Hoe.seq check
>06090_18387_B0Ho_1B_BSD_R1_B01_04_ABI24.ab1
    1 NNNNNNNNNN NNNNTNNNNN CCNGTNNNNN GCGGCCGTCG CTAGAGANAG CTGCGCTGGC
   61 GACGCTGTAG TCTTCAGAGA TGGGGNTGCT GTTGATTGTA GCCGTTGCTC TTTCAATGAG
  121 GGTGGATTCT TCTTGAGACA AAGGCTTGGC CATGGGCCCG GGATTTTCCT CCACGTCACC
  181 GCATGTTAGA AGACTTCCTC TGCCCTCGCC GGATCCCTTG TACAGCTCGT CCATGCCGAG
  241 AGTGATCCCG GCGGCGGTCA CGAACTCCAG CAGGACCATG TGATCGCGCT TCTCGTTGGG
  301 GTCTTTGCTC AGGGCGGACT GGTAGCTCAG GTAGTGGTTG TCGGGCAGCA GCACGGGGCC
  361 GTCGCCGATG GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC TGCACGCCGC CGTCCTCGAT
  421 GTTGTGGCGG ATCTTGAAGT TGGCCTTGAT GCCGTTCTTC TGCTTGTCGG CGGTGATATA
  481 GACGTTGTGG CTGTTGTAGT TGTACTCCAG CTTGTGCCCC AGGATGTTGC CGTCCTCCTT
  541 GAAGTCGATG CCCTTCAGCT CGATGCGGTT CACCAGGGTG TCGCCCTCGA ACTTCACCTC
  601 GGCGCGGGTC TTGTAGTTGC CGTCGTCCTT GAAGAAGATG GTGCGCTCCT GGACGTAGCC
  661 TTCGGGCATG GCGGACTTGA AGAAGTCGTG CTGCTTCATG TGGTCGGGGT AGCGGGCGAA
  721 GCACTGCAGG CCGTAGCCCA GGGTGGTCAC GANGGTGGGC CAGGGCACGG GCAGCTTGCC
  781 GGTGGTGCAG ATCAGCTTCA NGGTCAGCTT GCCGTNNNTG GCATCGCCCT CGCCNTCGCC
  841 NGANACGCTN AACTTGTGGC CGTTTACGTC NNCNNNCANC TCNNNNAGGN NNGGCACCAN
901 CCNCNNNNAA NANCTCCNCN NCNNNNCNCN CNNNNNNNNN N
//
Primer: bGH_rev3
Region: 3' LTR
Sequence file: RDB18387_B0Hof.seq check
>06090_18387_B0Ho_1B_bGH_rev3_G10_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG NNGGNNNNNN ACAGATGGCT GGCAACTAGA AGGCACAGTC
   61 GAGGCTGATC AGCGGGTTTA AACGGGCCCT GCTAGAGATT TTCNACACTG ACTAAAAGGG
  121 TCTGAGGGAT CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC
  181 TCAAGGCAAG CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA
  241 GGCTCAGATC TGGTCTAACC AGAGAGACCC AGTACAGTCC GGATGCAGCT CTCGGGCCAT
  301 GTGATGAAAT GCTAGGCGGC TGTCAAACCT CCACTCTAAT ACTTCTCTCT CCGGGTCATC
  361 CATCCCATGC AGGCTCACAG GGTGTAACAA GCGGGTGTTC TCTCCTTCAT TGGCTTCTTC
  421 TACCTTCTCT TGCTCAACTG GTACTAGCTT GTAGCACCAT CCAAAGGTCA GTGGATATCT
  481 GATCCCTGGC CCTGGTGTGT AGTTCTGCCA ATCAGGGAAG TAGCCTTGTG TGTGGTAGAT
  541 CCACAGATCA AGGATATCTT GTCTTCGTTG GGAGTGAATT AGCCCTTCCA GTCCCCCCTT
  601 TTCTTTTAAA AAGTGGCTAA GATCTACAGC TGCCTTGTAA GTCATTGGTC TTAAAGGTAC
  661 CTGANGTGTG ACTGGAAAAC CCACCTCCTC CTCCTCTTGT GCTTCTAGCC AGGCACAATC
  721 AGCATTGGTA GCTGCTGTAT TGCTACTTGT GATTGCTCCA TGTTTTTCTA GGTCTCGACG
  781 GTATCGATGC GGGGANGCGG CCNAAAGGGA NNTNNGNNTC GTCTGANGGC GAANGCGAAG
  841 NNGCNNNANA NGNCNCANAN CCNGCAGCNN NNCGCGGNAA GGNANNNTCC GCTNGNTTNA
901 NGNNCNNANN GNNNNNNNNN N
//
Primer: SV40pro_ori_F
Region: SV40 pro_ori,Zeo
Sequence file: RDB18387_B0Hog.seq check
>06090_18387_B0Ho_1B_SV40pro_ori_F_H10_22_ABI24.ab1
    1 NNNNNNNNNN NNNNNNCCTC TGCCTCTGNN CTNNTCNNGA ANTAGTGAGG ANGCTTTTTT
   61 GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT
  121 CAGCACGTGT TGACAATTAA TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG
  181 TGAGGAACTA AACCATGGCC AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG
  241 TCGCCGGAGC GGTCGAGTTC TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG
  301 ACGACTTCGC CGGTGTGGTC CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC
  361 AGGTGGTGCC GGACAACACC CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG
  421 CCGAGTGGTC GGAGGTCGTG TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG
  481 AGATCGGCGA GCAGCCGTGG GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG
  541 TGCACTTCGT GGCCGANGAG CAGGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG
  601 CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC
  661 AGCGCGGGGN NCTCATGCTG GANTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA
  721 ATGGTTACNA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC
  781 ATTCTANTTG TGGTTTGTCC NAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGNCNN
  841 NCTCTANCTA NANCTTGGNG NAANCATGGN CATANNNNNT NCCTGNNNGA AANTNNNNNC
  901 CGCNCNCNAT TNCNCACNNN NTNNNANNNN GNNNNNTNAN NNNNNANNNC NNNGGGNNNN
961 CNNNANNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

user report Hasegawa, K., YAP signaling induces PIEZO1 to promote oral squamous cell carcinoma cell proliferation. J. Pathol. 253 (1): 80-93 (2021). PMID 32985688.
user report Kamil, M., High filamin-C expression predicts enhanced invasiveness and poor outcome in glioblastoma multiforme. Br. J. Cancer. 120 (8): 819-826 (2019). PMID 30867563.
user report Makino, Y., Single cell RNA-sequencing identified Dec2 as a suppressive factor for spermatogonial differentiation by inhibiting Sohlh1 expression. Sci. Rep. 9 (1): 6063 (2019). PMID 30988352.
user report Yamagishi, M., Targeting Excessive EZH1 and EZH2 Activities for Abnormal Histone Methylation and Transcription Network in Malignant Lymphomas. Cell Rep. 29 (8): 2321-2337.e7 (2019). PMID 31747604.
user report Kimura, H., CKAP4 is a Dickkopf1 receptor and is involved in tumor progression. J. Clin. Invest. 126 (7): 2689-2705 (2016). PMID 27322059.
user report Matsumoto, S., A combination of Wnt and growth factor signaling induces Arl4c expression to form epithelial tubular structures. EMBO J., 33 (7): 702-718 (2014). PMID 24562386.
user report Yamagishi M, Polycomb-Mediated Loss of miR-31 Activates NIK-Dependent NF-κB Pathway in Adult T Cell Leukemia and Other Cancers. Cancer Cell, 21 (1): 121-135 (2012). PMID 22264793.

2021.02.02

GNP_filter3_RDBDEP_html_201222.pl