Resource data sheet
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CS-RfA-EVBsd

Backbone vector of shRNA expressing lentivirus construct by Gateway(R) cloning with Venus and Bsd marker driven by EF-1 alpha promoter.

Catalog number RDB06090
Resource name CS-RfA-EVBsd
Clone info. Backbone vector of shRNA expressing lentivirus construct by Gateway(R) cloning with Venus and Bsd marker driven by EF-1 alpha promoter.
Use DB3.1 (Invitrogen) or equivalent to amplify this clone. Commonly requested with pENTR4-H1 (RDB04395) for generation of shRNA expression vector and with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394) for packaging. Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone Plasmid
Selectable markers Amp^r, Cm^r, ccdB (E. coli); Zeo^r (plasmid transfection of mammalian cells); Bsd^r (lentivirus transfection).
Gene/insert name Aequorea victoria GFP cDNA
Originator Miyoshi, Hiroyuki |
CLICK here for plasmid list. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi (The Developer, the current address: Keio University) as the Developer. Dr. Miyoshi may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE.
Ordering Please visit Ordering instruction. 
Order form 
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Exclusive MTA [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06090 CS-RfA-EVBsd DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06090z.seq from Depositor
Electronic file PDF RDB06090.pdf from Depositor

Featured content

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12147_A5Dhp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxEF_F1 RDB12147_A5Dha.seq
>06090-012147_A5Dh_pAxEF_F1_D01_10.ab1
    1 ATTTTTCATC ATTTCAGTGT CGTGAATTAA TTAACCGGTT CCACAACCAT GGTGAGCAAG
   61 GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC
  121 GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC
  181 CTGAAGCTGA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC
  241 CTGGGCTACG GCCTGCAGTG CTTCGCCCGC TACCCCGACC ACATGAAGCA GCACGACTTC
  301 TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC
  361 GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC
  421 GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC
  481 AACTACAACA GCCACAACGT CTATATCACC GCCGACAAGC AGAAGAACGG CATCAAGGCC
  541 AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCGGCGTGC AGCTCGCCGA CCACTACCAG
  601 CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCTAC
  661 CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC
  721 GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGGGATC CGGCGAGGGC
  781 AGAGGAAGTC TTCTAACATG CGGTGACGTG GAGGAAAATC CCGGGCCCAT GGCCAAGCCT
  841 TTGTCTCAAG AAGAATCCAC CCTCATTGAA AGAGCAACGG CTACAATCAA CAGCATCCCC
  901 ATCTCTGAAG ACTACAGCGT CGCCAGCGCA GCTCTCTCTA GCGACGGCCG CATCTTCACT
  961 GGTGTCAATG TATATCATTT TACTGGGGGG ACCTTGTGCA GAACTCGTGG TGCTGGGCAC
 1021 TGCTGCTGCT GCGCAGCTGG CACTGACTTG TATCGTCGCG ATCGGAATGA GACCAGGGCC
 1081 ATCTGAGCTC CTGCTACGTG CCGAACGTGC TCTCGATCTG CCATCTGGGG AATCAAAGGT
1141 CCAATAATGT GAG
//
Primer: Cm_M_R RDB12147_A5Dhb.seq
>06090_12147_A5Dh_Cm_M_R_A07_01.ab1
    1 GCTTGGAATG ACCTATCCAT ATCACCAGCT CACCGTCTTT CATTGCCATA CGGAATTCCG
   61 GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC TTGTGCTTAT
  121 TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG TTATAGGTAC
  181 ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG GATATATCAA
  241 CGGTGGTATA TCCAGTGATT TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT GAAAATCTCG
  301 ACGGATCCTA ACTCAAAATC CACACATTAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG
  361 GGGTGCCTAA TGCGGCCGCC ATAGTGACTG GATATGTTGT GTTTTACAGT ATTATGTAGT
  421 CTGTTTTTTA TGCAAAATCT AATTTAATAT ATTGATATTT ATATCATTTT ACGTTTCTCG
  481 TTCAGCTTTT TTGTACAAAC TTGTGATAAT TCAAAGTGAA TTTCTGCTGT CCCTGTAATA
  541 AACCCGAAAA TTTTGAATTT TTGTAATTTG TTTTTGTAAT TCTTTAGTTT GTATGTCTGT
  601 TGCTATTATG TCTACTATTC TTTCCCCTGC ACTGTACCCC CCAATCCCCC CTTTTCTTTT
  661 AAAATTGTGG ATGAATACTG CCATTTGTAG ATCCGTTCAC TAATCGAATG GATCTGTCTC
  721 TGTCTCTCTC TCCACCTTCT TCTTCTATTC CTTCGGGCCT GTCGGGTCCC CTCGGGGTTG
  781 GGAGGTGGGT CTGAAACGAT AATGGTGAAT ATCCCTGCCT AACTCTATTC ACTATAGAAA
  841 GTACAGCAAA AAACTATTCT TAAACCTACC AAGCCTCCTA CTATCATTAT GAATAATTTT
  901 ATATACCACA GCCCAATTTG TTATGTTAAC CAATTTCCAC AACTTGCCCA TTTATCTAAT
  961 TCCAATATTC TGTTCATTCT TTTCTTGGCT GATTTGCGAT TCTTCATTAA GAGTGTAATA
 1021 AGCTTGTTGT AATGTAATTT CTCTGGTCCC ACTCATCCAG TTCGTTGATT CGAATCTTGT
1081 TTCCGAGAAA GAA
//
Primer: EF1a-N_R RDB12147_A5Dhc.seq
>06090_12147_A5Dh_EF1a-N_R_F01_16.ab1
    1 ACTTCTGGGG GGATGTGGGC GATGTGCGCT CTGCCCACTG ACGGGCACCG GAGCCTCACG
   61 AAGCTTGAAT TATCACCACT TTGTACAAGA AAGCTGAACG AGAAACGTAA AATGATATAA
  121 ATATCAATAT ATTAAATTAG ATTTTGCATA AAAAACAGAC TACATAATAC TGTAAAACAC
  181 AACATATCCA GTCACTATGG TCGACCTGCA GACTGGCTGT GTATAAGGGA GCCTGACATT
  241 TATATTCCCC AGAACATCAG GTTAATGGCG TTTTTGATGT CATTTTCGCG GTGGCTGAGA
  301 TCAGCCACTT CTTCCCCGAT AACGGAGACC GGCACACTGG CCATATCGGT GGTCATCATG
  361 CGCCAGCTTT CATCCCCGAT ATGCACCACC GGGTAAAGTT CACGGGAGAC TTTATCTGAC
  421 AGCAGACGTG CACTGGCCAG GGGGATCACC ATCCGTCGCC CGGGCGTGTC AATAATATCA
  481 CTCTGTACAT CCACAAACAG ACGATAACGG CTCTCTCTTT TATAGGTGTA AACCTTAAAC
  541 TGCATTTCAC CAGCCCCTGT TCTCGTCAGC AAAAGAGCCG TTCATTTCAA TAAACCGGGC
  601 GACCTCAGCC ATCCCTTCCT GATTTTCCGC TTTCCAGCGT TCGGCACGCA GACGACGGGC
  661 TTCATTCTGC ATGGTTGTGC TTACCAGACC GGAGATATTG ACATCATATA TGCCTTGAGC
  721 AACTGATAGC TGTCGCTGTC AACTGTCACT GTAATACGCT GCTTCATAGC ATACCTCTTT
  781 TTTGACATAC TTCGGGTATA CATATCAGTA TATATTCTTA TACCGCAAAA ATCAGCGCGC
  841 AAATACGCAT ACTGTTATCT GGCTTTTAGT AAGCCGGGAT CCACGCGTTT ACGCCCCGCC
  901 CTGCCACTCA TCGCAGTACT GTTGTAATTC ATTAAGCATT CTGCGACATG GTAGGCAATC
  961 ACAAACGCAT GATGGATCTT GATTCGGCCA GCGCATCAGC AACTGTCGCT GCGTATAATA
1021 TTTGGTCATG CTGAACGAGC CGAGAGTGTC GATATTGACA CGTTAATTCA GA
//

Please visit Sequencing and PCR primers for primer information.


References

References

user report Kimura, H., CKAP4 is a Dickkopf1 receptor and is involved in tumor progression. J. Clin. Invest. 126 (7): 2689-2705 (2016). PMID 27322059.
user report Matsumoto, S., A combination of Wnt and growth factor signaling induces Arl4c expression to form epithelial tubular structures. EMBO J., 33 (7): 702-718 (2014). PMID 24562386.
user report Yamagishi M, Polycomb-Mediated Loss of miR-31 Activates NIK-Dependent NF-κB Pathway in Adult T Cell Leukemia and Other Cancers. Cancer Cell, 21 (1): 121-135 (2012). PMID 22264793.

2018.10.22

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