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CS-RfA-EVBsd (#RDB06090)

Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with Venus and Bsd marker driven by EF-1 alpha promoter.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Unpublished bioresource
Test sheet Data Sheet open 
 
Clone info. Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with Venus and Bsd marker driven by EF-1 alpha promoter.
Comment Commonly requested with pENTR4-H1 (RDB04395) for generation of shRNA expression vector and with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394) for packaging.
Vector backbone Plasmid
Selectable markers Ampicillin, Chloramphenicol, ccdB (E. coli), Bsd (lentivirus transfection). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth conditions 37C, LB+Amp
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Miyoshi, Hiroyuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
付加的使用条件：
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。

Catalog # Resource name Shipping form Fee
RDB06090 CS-RfA-EVBsd DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Ordering instruction of plasmids [in Japanese] [in English]

How to cite this biological resource

Materials & Methods section:

The CS-RfA-EVBsd was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06090).

Reference section:

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

user_report Yamagishi, M., Mechanisms of action and resistance in histone methylation-targeted therapy. Nature 627 (8002): 221-228 (2024). PMID 38383791. [PubMed] [Article] [RRC of NBRP]
user_report Araki, H., LSD1 defines the fiber type-selective responsiveness to environmental stress in skeletal muscle. Elife 12: e84618 (2023). PMID 36695573. [PubMed] [Article] [RRC of NBRP]
user_report Truong, T.T.K., Arl4c is involved in tooth germ development through osteoblastic/ameloblastic differentiation. Biochem. Biophys. Res. Commun. 679: 167-174 (2023). PMID 37703759. [PubMed] [Article] [RRC of NBRP]
user_report Hada, M., Highly rigid H3.1/H3.2-H3K9me3 domains set a barrier for cell fate reprogramming in trophoblast stem cells. Genes Dev. 36 (1-2): 84-102 (2022). PMID 34992147. [PubMed] [Article] [RRC of NBRP]
user_report Hasegawa, K., YAP signaling induces PIEZO1 to promote oral squamous cell carcinoma cell proliferation. J. Pathol. 253 (1): 80-93 (2021). PMID 32985688. [PubMed] [Article] [RRC of NBRP]
user_report Nakashima, M., Differentiation of Hodgkin lymphoma cells by reactive oxygen species and regulation by heme oxygenase-1 through HIF-1α. Cancer Sci. 112 (6): 2542-2555 (2021). PMID 33738869. [PubMed] [Article] [RRC of NBRP]
user_report Kamil, M., High filamin-C expression predicts enhanced invasiveness and poor outcome in glioblastoma multiforme. Br. J. Cancer. 120 (8): 819-826 (2019). PMID 30867563. [PubMed] [Article] [RRC of NBRP]
user_report Makino, Y., Single cell RNA-sequencing identified Dec2 as a suppressive factor for spermatogonial differentiation by inhibiting Sohlh1 expression. Sci. Rep. 9 (1): 6063 (2019). PMID 30988352. [PubMed] [Article] [RRC of NBRP]
user_report Yamagishi, M., Targeting Excessive EZH1 and EZH2 Activities for Abnormal Histone Methylation and Transcription Network in Malignant Lymphomas. Cell Rep. 29 (8): 2321-2337.e7 (2019). PMID 31747604. [PubMed] [Article] [RRC of NBRP]
user_report Kimura, H., CKAP4 is a Dickkopf1 receptor and is involved in tumor progression. J. Clin. Invest. 126 (7): 2689-2705 (2016). PMID 27322059. [PubMed] [Article] [RRC of NBRP]
user_report Matsumoto, S., A combination of Wnt and growth factor signaling induces Arl4c expression to form epithelial tubular structures. EMBO J., 33 (7): 702-718 (2014). PMID 24562386. [PubMed] [Article] [RRC of NBRP]
user_report Yamagishi M, Polycomb-Mediated Loss of miR-31 Activates NIK-Dependent NF-κB Pathway in Adult T Cell Leukemia and Other Cancers. Cancer Cell, 21 (1): 121-135 (2012). PMID 22264793. [PubMed] [Article] [RRC of NBRP]