Sequencing and PCR primers for primer information.
Expression vector of lacZ, with FLAG-tag, control vector
| Catalog number | RDB06072 |
|---|---|
| Resource name | pCMFlag_lacZ |
| Alternative name | SET-0109_5 |
| Clone info. | Expression vector of E. coli lacZ. Expression was confirmed by Western blotting with anti-FLAG antibody. PCR template： pcDNA4/HisMax/lacZ |
| Vector backbone | pCMV_S-FLAG (Plasmid) |
| Size of vector backbone | 5.5 kb |
| Selectable markers | Amp^r (E. coli), Neo^r (mammalian cell) |
| Gene/insert name | E. coli lacZ (beta-D-galactosidase) |
| Depositor | DNA Bank, | |
| Other clones in our bank | E. coli lacZ (KEGG orthology K01190) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL in any publication. |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB06072 | pCMFlag_lacZ | DNA solution |
Electronic file
| Electronic file | PDF RDB06072.pdf from Depositor |
|---|
Featured content
| Featured content | pCMV_tag and pRSV_tag assay-ready clones (English text) |
|---|---|
| Featured content | pCMV_tag and pRSV_tag vector (English text) |
| Featured content | Control Vectors (English text) |
| Featured content | pCMV_tag and pRSV_tag vector (Japanese text) |
References
User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB14342_A4Iap1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: CMV-Forward | RDB14342_A4Iaa.seq |
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>RDB6072_3_CMV_Forward_C05_08.ab1 1 TCGGATCCAC CTCCGGCCCC ATTGACGCAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT 61 ATATAAGCAG AGCTCTCTGG CTAACTAGAG AACCCACTGC TTACTGGCTT ATCGAAATTA 121 ATACGACTCA CTATAGGGAG ACCCAAGCTG AATTCACCAT GGACTACAAG GACGATGACG 181 ATAAGGCGGC CGATCCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT GGCGTTACCC 241 AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC 301 GCACCGATCG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGCGC TTTGCCTGGT 361 TTCCGGCACC AGAAGCGGTG CCGGAAAGCT GGCTGGAGTG CGATCTTCCT GAGGCCGATA 421 CTGTCGTCGT CCCCTCAAAC TGGCAGATGC ACGGTTACGA TGCGCCCATC TACACCAACG 481 TGACCTATCC CATTACGGTC AATCCGCCGT TTGTTCCCAC GGAGAATCCG ACGGGTTGTT 541 ACTCGCTCAC ATTTAATGTT GATGAAAGCT GGCTACAGGA AGGCCAGACG CGAATTATTT 601 TTGATGGCGT TAACTCGGCG TTTCATCTGT GGTGCAACGG GCGCTGGGTC GGTTACGGCC 661 AGGACAGTCG TTTGCCGTCT GAATTTGACC TGAGCGCATT TTTACGCGCC GGAGAAAACC 721 GCCTCGCGGT GATGGTGCTG CGCTGGAGTG ACGGCAGTTA TCTGGAAGAT CAGGATATGT 781 GGCGGATGAG CGGCATTTTC CGTGACGTCT CGTTGCTGCA TAAACCGACT ACACAAATCA 841 GCGATTTCCA TGTTGCCACT CGCTTTAATG ATGATTTCAG CCGCGCTGTA CTGGAGGCTG 901 AAGTTCAGAT GTGCGGCGAG TTGCGTGACT ACCTACGGGT AACAGTTTCT TTATGGCAGG 961 GTGAAACGCA GTCGCCAGCG GGCACCGCGC CTTTCGGCGG TGAAATTATC GATGAGCGTG 1021 GTGGTTTATG CCCGATCGCG TCACACTACG TCTGACGTTC GAAACCCGAA CTGGTGAGCG 1081 CCGATCCGAT CTCTATCGTG CGTGATGAAC CTGCAACTGT CGACGACACG CATGAGTTCC 1141 TAGC // |
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| Primer: BGH_rev | RDB14342_A4Iab.seq |
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>RDB6072_3_BGH_rev_D05_11.ab1 1 GTTCTAGTCA TTAAGGTGAA CTATAGAATA GGGCCCTCTA GATGCATGCT CGAGCGGCCT 61 TATTTTTGAC ACCAGACCAA CTGGTAATGG TAGCGACCGG CGCTCAGCTG TAATTCCGCC 121 GATACTGACG GGCTCCAGGA GTCGTCTCCA CCAATCCCCA TATGGAAACC GTCGATATTC 181 AGCCATGTGC CTTCTTCCGC GTGCAGCAGA TGGCGATGGC TGGTTTCCAT CAGTTGCTGT 241 TGACTGTAGC GGCTGATGTT GAACTGGAAG TCGCCGCGCC ACTGGTGTGG GCCATAATTC 301 AATTCGCGCG TCCCGCAGCG CAGACCGTTT TCGCTCGGGA AGACGTACGG GGTATACATG 361 TCTGACAATG GCAGATCCCA GCGGTCAAAA CAGGCGGCAG TAAGGCGGTC GGGATAGTTT 421 TCTTGCGGCC CTAATCCGAG CCAGTTTACC CGCTCTGCTA CCTGCGCCAG CTGGCAGTTC 481 AGGCCAATCC GCGCCGGATG CGGTGTATCG CTCGCCACTT CAACATCAAC GGTAATCGCC 541 ATTTGACCAC TACCATCAAT CCGGTAGGTT TTCCGGCTGA TAAATAAGGT TTTCCCCTGA 601 TGCTGCCACG CGTGAGCGGT CGTAATCAGC ACCGCGTCAG CAAGTGTATC TGCCGTGCAC 661 TGCAACAACG CTGCTTCGGC CTGGTAATGG CCCGCCGCCT TCCAGCGTTC GACCCAGGCG 721 TTAGGGTCAA TGCGGGTCGC TTCACTTACG CCAATGTCGT TATCCAGCGG TGCACGGGTG 781 AACTGATCGC GCAGCGGCGT CAGCAGTTGT TTTTTATCGC CAATCCACAT CTGTGAAAGA 841 AAGCCTGACT GGCGGTTAAA TTGCCAACGC TTATTACCCA GCTCGATGCA AAAATCCATT 901 TCGCTGGTGG TCAGATGCGG GATGGCGTGG GACGCGGCGG GGAGCGTCAC ACTGAGGTTT 961 TCCGCCAGAC GCCACTGCTG CCAGGCGCTG ATGTGGCCCG GCTTTCTGAC CATGCGGGTC 1021 GCGTTTCGGG TTGCACTACG CGTACTGTGA GCCAGAGGTT GCCCCGGCCG CCTTCTCGGC 1081 TTGCGGTTAG TTCAGGCCAG TTCCATCCAC CTGGTTTACC CTTGTTGGAG CCGACCATTC 1141 CCAGAAAGGC AACTTTTCAA CCGG // |
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| Primer: SV40pro_F_V2 | RDB14342_A4Iac.seq |
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>RDB6072_3_SV40pro_F_V2_G04_19.ab1 1 GACGGCTTTT TGGAGCTAGG CTTTTGCAAA AGCTCCCGGG AGCTTGGATA TCCATTTTCG 61 GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG 121 CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA 181 TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG 241 TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT 301 GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA 361 GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC 421 CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG 481 CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG 541 AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG 601 AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG 661 GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT 721 GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGACATAGCG TTGGCTACCC GTGATATTGC 781 TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC 841 CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG 901 GGGTTCGAAA TGACGACAAG CGACGCCACT GCATCACGAG ATTCGATCAC GCGCTTCTAT 961 GAAAGTGGCT TCGATCGTTT CAGACGCGCT GATGATCCTC CAGCGCGGGG ATCTCATGCT 1021 GGAGTTCTTC GCCCACCCAC TTGTTTTATG CAGCTTATAA TGGTACAATT AAGGCCATAG 1081 GCATCCAATT TCCAAATAAG CATTTTCATG CATTCATTGT GTCAACCTAT CAGGATCCCT 1141 A // |
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Please visit Sequencing and PCR primers for primer information.
References
2018.10.22
GNP_filter3_RDBDEP_html_181012.pl