Resource data sheet
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pCMFlag_lacZ

Expression vector of lacZ, with FLAG-tag, control vector

Catalog number RDB06072
Resource name pCMFlag_lacZ
Alternative name SET-0109_5
Clone info. Expression vector of E. coli lacZ. Expression was confirmed by Western blotting with anti-FLAG antibody.
PCR template: pcDNA4/HisMax/lacZ
Vector backbone pCMV_S-FLAG (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name E. coli lacZ (beta-D-galactosidase)
Depositor DNA Bank, |
 
Other clones in our bank E. coli lacZ (KEGG orthology K01190) |

Distribution information

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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06072 pCMFlag_lacZ DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file PDF RDB06072.pdf from Depositor

Featured content

Featured content pCMV_tag and pRSV_tag assay-ready clones (English text)
Featured content pCMV_tag and pRSV_tag vector (English text)
Featured content Control Vectors (English text)
Featured content pCMV_tag and pRSV_tag vector (Japanese text)

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14342_A4Iap1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB14342_A4Iaa.seq
>RDB6072_3_CMV_Forward_C05_08.ab1
    1 TCGGATCCAC CTCCGGCCCC ATTGACGCAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT
   61 ATATAAGCAG AGCTCTCTGG CTAACTAGAG AACCCACTGC TTACTGGCTT ATCGAAATTA
  121 ATACGACTCA CTATAGGGAG ACCCAAGCTG AATTCACCAT GGACTACAAG GACGATGACG
  181 ATAAGGCGGC CGATCCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT GGCGTTACCC
  241 AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC
  301 GCACCGATCG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGCGC TTTGCCTGGT
  361 TTCCGGCACC AGAAGCGGTG CCGGAAAGCT GGCTGGAGTG CGATCTTCCT GAGGCCGATA
  421 CTGTCGTCGT CCCCTCAAAC TGGCAGATGC ACGGTTACGA TGCGCCCATC TACACCAACG
  481 TGACCTATCC CATTACGGTC AATCCGCCGT TTGTTCCCAC GGAGAATCCG ACGGGTTGTT
  541 ACTCGCTCAC ATTTAATGTT GATGAAAGCT GGCTACAGGA AGGCCAGACG CGAATTATTT
  601 TTGATGGCGT TAACTCGGCG TTTCATCTGT GGTGCAACGG GCGCTGGGTC GGTTACGGCC
  661 AGGACAGTCG TTTGCCGTCT GAATTTGACC TGAGCGCATT TTTACGCGCC GGAGAAAACC
  721 GCCTCGCGGT GATGGTGCTG CGCTGGAGTG ACGGCAGTTA TCTGGAAGAT CAGGATATGT
  781 GGCGGATGAG CGGCATTTTC CGTGACGTCT CGTTGCTGCA TAAACCGACT ACACAAATCA
  841 GCGATTTCCA TGTTGCCACT CGCTTTAATG ATGATTTCAG CCGCGCTGTA CTGGAGGCTG
  901 AAGTTCAGAT GTGCGGCGAG TTGCGTGACT ACCTACGGGT AACAGTTTCT TTATGGCAGG
  961 GTGAAACGCA GTCGCCAGCG GGCACCGCGC CTTTCGGCGG TGAAATTATC GATGAGCGTG
 1021 GTGGTTTATG CCCGATCGCG TCACACTACG TCTGACGTTC GAAACCCGAA CTGGTGAGCG
 1081 CCGATCCGAT CTCTATCGTG CGTGATGAAC CTGCAACTGT CGACGACACG CATGAGTTCC
1141 TAGC
//
Primer: BGH_rev RDB14342_A4Iab.seq
>RDB6072_3_BGH_rev_D05_11.ab1
    1 GTTCTAGTCA TTAAGGTGAA CTATAGAATA GGGCCCTCTA GATGCATGCT CGAGCGGCCT
   61 TATTTTTGAC ACCAGACCAA CTGGTAATGG TAGCGACCGG CGCTCAGCTG TAATTCCGCC
  121 GATACTGACG GGCTCCAGGA GTCGTCTCCA CCAATCCCCA TATGGAAACC GTCGATATTC
  181 AGCCATGTGC CTTCTTCCGC GTGCAGCAGA TGGCGATGGC TGGTTTCCAT CAGTTGCTGT
  241 TGACTGTAGC GGCTGATGTT GAACTGGAAG TCGCCGCGCC ACTGGTGTGG GCCATAATTC
  301 AATTCGCGCG TCCCGCAGCG CAGACCGTTT TCGCTCGGGA AGACGTACGG GGTATACATG
  361 TCTGACAATG GCAGATCCCA GCGGTCAAAA CAGGCGGCAG TAAGGCGGTC GGGATAGTTT
  421 TCTTGCGGCC CTAATCCGAG CCAGTTTACC CGCTCTGCTA CCTGCGCCAG CTGGCAGTTC
  481 AGGCCAATCC GCGCCGGATG CGGTGTATCG CTCGCCACTT CAACATCAAC GGTAATCGCC
  541 ATTTGACCAC TACCATCAAT CCGGTAGGTT TTCCGGCTGA TAAATAAGGT TTTCCCCTGA
  601 TGCTGCCACG CGTGAGCGGT CGTAATCAGC ACCGCGTCAG CAAGTGTATC TGCCGTGCAC
  661 TGCAACAACG CTGCTTCGGC CTGGTAATGG CCCGCCGCCT TCCAGCGTTC GACCCAGGCG
  721 TTAGGGTCAA TGCGGGTCGC TTCACTTACG CCAATGTCGT TATCCAGCGG TGCACGGGTG
  781 AACTGATCGC GCAGCGGCGT CAGCAGTTGT TTTTTATCGC CAATCCACAT CTGTGAAAGA
  841 AAGCCTGACT GGCGGTTAAA TTGCCAACGC TTATTACCCA GCTCGATGCA AAAATCCATT
  901 TCGCTGGTGG TCAGATGCGG GATGGCGTGG GACGCGGCGG GGAGCGTCAC ACTGAGGTTT
  961 TCCGCCAGAC GCCACTGCTG CCAGGCGCTG ATGTGGCCCG GCTTTCTGAC CATGCGGGTC
 1021 GCGTTTCGGG TTGCACTACG CGTACTGTGA GCCAGAGGTT GCCCCGGCCG CCTTCTCGGC
 1081 TTGCGGTTAG TTCAGGCCAG TTCCATCCAC CTGGTTTACC CTTGTTGGAG CCGACCATTC
1141 CCAGAAAGGC AACTTTTCAA CCGG
//
Primer: SV40pro_F_V2 RDB14342_A4Iac.seq
>RDB6072_3_SV40pro_F_V2_G04_19.ab1
    1 GACGGCTTTT TGGAGCTAGG CTTTTGCAAA AGCTCCCGGG AGCTTGGATA TCCATTTTCG
   61 GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG
  121 CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA
  181 TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG
  241 TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT
  301 GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA
  361 GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC
  421 CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG
  481 CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG
  541 AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG
  601 AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG
  661 GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT
  721 GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGACATAGCG TTGGCTACCC GTGATATTGC
  781 TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC
  841 CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG
  901 GGGTTCGAAA TGACGACAAG CGACGCCACT GCATCACGAG ATTCGATCAC GCGCTTCTAT
  961 GAAAGTGGCT TCGATCGTTT CAGACGCGCT GATGATCCTC CAGCGCGGGG ATCTCATGCT
 1021 GGAGTTCTTC GCCCACCCAC TTGTTTTATG CAGCTTATAA TGGTACAATT AAGGCCATAG
 1081 GCATCCAATT TCCAAATAAG CATTTTCATG CATTCATTGT GTCAACCTAT CAGGATCCCT
1141 A
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.10.22

GNP_filter3_RDBDEP_html_181012.pl