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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCMFlag_EGFP

Expression vector of GFP, with FLAG-tag, control vector.

Catalog number RDB06071
Resource name pCMFlag_EGFP
Alternative name SET-0108_4
Clone info. Expression vector of EGFP. Expression was confirmed by Western blotting with anti-FLAG antibody.
PCR amplified from a GFP expression vector.
Vector backbone pCMV_S-FLAG (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Aequorea victoria GFP cDNA
Depositor DNA Bank, |

Distribution information

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Schedule A (GFP-A) [Link]
Remarks
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06071 pCMFlag_EGFP DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content pCMV_tag and pRSV_tag assay-ready clones (English text)
Featured content GFP Resources (English text)
Featured content Empty Backbone (English text)
Featured content Empty Backbone (Japanese text)
Featured content pCMV_tag and pRSV_tag vector (English text)
Featured content pCMV_tag and pRSV_tag vector (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13312_A5DKp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: CMV pro, FLAG, EGFP, bGHp(A)
Sequence file: RDB13312_A5Dka.seq check
>06071_13312_A5Dk_CMV-Forward_A06_03.ab1
    1 CTTTCAACTC GCCCCATTGA CGCAAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
   61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC
  121 GACTCACTAT AGGGAGACCC AAGCTGAATT CACCATGGAC TACAAGGACG ATGACGATAA
  181 GGCGGCCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA
  241 GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC
  301 CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG
  361 GCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA
  421 CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC
  481 CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA
  541 CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT
  601 GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA
  661 GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA
  721 GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA
  781 CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA
  841 CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA
  901 CAAGTCCGGA CTCAGATCTC GAGCTCAAGC TTCGAATTCT GCAGTCGACG GTACCGCGGG
  961 CCCGGGGATC CACCGGATCT AGATAAGGCC GCTCGAGCAT GCATCTAGAG GGCCCTATTC
 1021 TATAGTGTCA CCTAAATGCT AGAGCTCGCT GATCAGCCCT CGACTGTGCC TTCTAGTTTG
 1081 CCAGCCATCC TGCTGCTTGG CCCCTCCCCC GAGCCCGTTC CTTGGAGCCT GGAAGGGTGG
1141 GC
//
Primer: SV40pro_F_V2
Region: SV40 pro_ori, NeoR
Sequence file: RDB13312_A5Dkb.seq check
>06071_13312_A5Dk_SV40pro_F_V2_B06_06.ab1
    1 TGAAACTTTT TTGGAGGCTA GGCTTTTGCA AAAGCTCCCG GGAGCTTGGA TATCCATTTT
   61 CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA
  121 CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC
  181 AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT
  241 TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC
  301 GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG
  361 AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC
  421 TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC
  481 GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT
  541 GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC
  601 CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA
  661 TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA
  721 CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT
  781 TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC
  841 TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT
  901 CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC
  961 ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TTCCGGGACG CCCGGCTGGA
 1021 TGATCCTCCC AGCGCGGGGA TCTCATGCTG CAGTTCTTTC GCCCCACCCC ACTTGTTTAT
 1081 TGCAGCTTTA TATTGGTACG ATTAGGCCAT AGCCATCACA AAAGTTTCAC AATTAAAAGG
1141 CCAA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles


2020.01.04

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