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Expression vector of GFP, with FLAG-tag, control vector.
| Catalog number | RDB06071 |
|---|---|
| Resource name | pCMFlag_EGFP |
| Alternative name | SET-0108_4 |
| Clone info. | Expression vector of EGFP. Expression was confirmed by Western blotting with anti-FLAG antibody. PCR amplified from a GFP expression vector. |
| Vector backbone | pCMV_S-FLAG (Plasmid) |
| Size of vector backbone | 5.5 kb |
| Selectable markers | Amp^r (E. coli), Neo^r (mammalian cell) |
| Gene/insert name | Aequorea victoria GFP cDNA |
| Depositor|Developer | DNA Bank, | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL in any publication. |
|---|---|
| Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment ] [Bank Transfer or Check Payment]Material Transfer Agreement (MTA) [Word ]Schedule A (GFP-A) [Link] |
| Remarks | Please use Schedule A (GFP-A) additionally. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB06071 | pCMFlag_EGFP | DNA solution |
icon below as well as clone information before placing your order.Electronic file
Original reference
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | pCMV_tag and pRSV_tag assay-ready clones (English text) |
|---|---|
| Featured content | GFP Resources (English text) |
| Featured content | Empty Backbone (English text) |
| Featured content | Empty Backbone (Japanese text) |
| Featured content | pCMV_tag and pRSV_tag vector (English text) |
| Featured content | pCMV_tag and pRSV_tag vector (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB13312_A5DKp1-2.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: CMV_Forward Region: CMV pro, FLAG, EGFP, bGHp(A) Sequence file: RDB13312_A5Dka.seq
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>06071_13312_A5Dk_CMV-Forward_A06_03.ab1 1 CTTTCAACTC GCCCCATTGA CGCAAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT 61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC 121 GACTCACTAT AGGGAGACCC AAGCTGAATT CACCATGGAC TACAAGGACG ATGACGATAA 181 GGCGGCCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA 241 GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC 301 CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG 361 GCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA 421 CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC 481 CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA 541 CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT 601 GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA 661 GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA 721 GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA 781 CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA 841 CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA 901 CAAGTCCGGA CTCAGATCTC GAGCTCAAGC TTCGAATTCT GCAGTCGACG GTACCGCGGG 961 CCCGGGGATC CACCGGATCT AGATAAGGCC GCTCGAGCAT GCATCTAGAG GGCCCTATTC 1021 TATAGTGTCA CCTAAATGCT AGAGCTCGCT GATCAGCCCT CGACTGTGCC TTCTAGTTTG 1081 CCAGCCATCC TGCTGCTTGG CCCCTCCCCC GAGCCCGTTC CTTGGAGCCT GGAAGGGTGG 1141 GC // |
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Primer: SV40pro_F_V2 Region: SV40 pro_ori, NeoR Sequence file: RDB13312_A5Dkb.seq
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>06071_13312_A5Dk_SV40pro_F_V2_B06_06.ab1 1 TGAAACTTTT TTGGAGGCTA GGCTTTTGCA AAAGCTCCCG GGAGCTTGGA TATCCATTTT 61 CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA 121 CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC 181 AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT 241 TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC 301 GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG 361 AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC 421 TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC 481 GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT 541 GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC 601 CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA 661 TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA 721 CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT 781 TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC 841 TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT 901 CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC 961 ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TTCCGGGACG CCCGGCTGGA 1021 TGATCCTCCC AGCGCGGGGA TCTCATGCTG CAGTTCTTTC GCCCCACCCC ACTTGTTTAT 1081 TGCAGCTTTA TATTGGTACG ATTAGGCCAT AGCCATCACA AAAGTTTCAC AATTAAAAGG 1141 CCAA // |
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Sequencing and PCR primers for primer information.
Original, user report and related articles
2020.10.02
GNP_filter3_RDBDEP_html_200316.pl