Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCMFlag_EGFP (#RDB06071)

Expression vector of GFP, with FLAG-tag, control vector.

Alternative name	SET-0108_4
Clone info.	Expression vector of EGFP. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment	PCR amplified from a GFP expression vector.
Vector backbone	pCMV_S-FLAG (RDB05956) (Plasmid)
Size of vector backbone	5.5 kb
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name	Aequorea victoria GFP cDNA
Depositor|Developer	DNA Bank, |

 

Distribution information

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提供案内 (日本国内) [open/close]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB06071	pCMFlag_EGFP	DNA solution

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The pCMFlag_EGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06071).

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13312_A5DKp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro, FLAG, EGFP, bGHp(A) Sequence file: RDB13312_A5Dka.seq
>06071_13312_A5Dk_CMV-Forward_A06_03.ab1     1 CTTTCAACTC GCCCCATTGA CGCAAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT    61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC   121 GACTCACTAT AGGGAGACCC AAGCTGAATT CACCATGGAC TACAAGGACG ATGACGATAA   181 GGCGGCCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA   241 GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC   301 CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG   361 GCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA   421 CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC   481 CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA   541 CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT   601 GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA   661 GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA   721 GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA   781 CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA   841 CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA   901 CAAGTCCGGA CTCAGATCTC GAGCTCAAGC TTCGAATTCT GCAGTCGACG GTACCGCGGG   961 CCCGGGGATC CACCGGATCT AGATAAGGCC GCTCGAGCAT GCATCTAGAG GGCCCTATTC  1021 TATAGTGTCA CCTAAATGCT AGAGCTCGCT GATCAGCCCT CGACTGTGCC TTCTAGTTTG  1081 CCAGCCATCC TGCTGCTTGG CCCCTCCCCC GAGCCCGTTC CTTGGAGCCT GGAAGGGTGG 1141 GC //
Primer: SV40pro_F_V2 (Pr0154) Region: SV40 pro_ori, NeoR Sequence file: RDB13312_A5Dkb.seq
>06071_13312_A5Dk_SV40pro_F_V2_B06_06.ab1     1 TGAAACTTTT TTGGAGGCTA GGCTTTTGCA AAAGCTCCCG GGAGCTTGGA TATCCATTTT    61 CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA   121 CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC   181 AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT   241 TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC   301 GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG   361 AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC   421 TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC   481 GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT   541 GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC   601 CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA   661 TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA   721 CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT   781 TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC   841 TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT   901 CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC   961 ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TTCCGGGACG CCCGGCTGGA  1021 TGATCCTCCC AGCGCGGGGA TCTCATGCTG CAGTTCTTTC GCCCCACCCC ACTTGTTTAT  1081 TGCAGCTTTA TATTGGTACG ATTAGGCCAT AGCCATCACA AAAGTTTCAC AATTAAAAGG 1141 CCAA //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

Resource search

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External Database
Aequorea victoria GFP

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Reference sequence
  

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