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pCMFlag_EGFP

Expression vector of GFP, with FLAG-tag, control vector.

Catalog number RDB06071
Resource name pCMFlag_EGFP
Alternative name SET-0108_4
Clone info. Expression vector of EGFP. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment PCR amplified from a GFP expression vector.
Vector backbone pCMV_S-FLAG (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Aequorea victoria GFP
            Reference sequence
              
             

            Distribution information

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            RDB06071 pCMFlag_EGFP DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pCMFlag_EGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06071).

            Reference section:

            Further references such as user reports and related articles (go to bottom)

            References and tips

            Featured content

            Featured content pCMV_tag and pRSV_tag assay-ready clones (English text)
            Featured content GFP Resources (English text)
            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)
            Featured content pCMV_tag and pRSV_tag vector (English text)
            Featured content pCMV_tag and pRSV_tag vector (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13312_A5DKp1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CMV_Forward (Pr0016)
            Region: CMV pro, FLAG, EGFP, bGHp(A)
            Sequence file: RDB13312_A5Dka.seq check
            >06071_13312_A5Dk_CMV-Forward_A06_03.ab1
                1 CTTTCAACTC GCCCCATTGA CGCAAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
               61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC
              121 GACTCACTAT AGGGAGACCC AAGCTGAATT CACCATGGAC TACAAGGACG ATGACGATAA
              181 GGCGGCCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA
              241 GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC
              301 CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG
              361 GCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA
              421 CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC
              481 CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA
              541 CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT
              601 GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA
              661 GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA
              721 GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA
              781 CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA
              841 CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA
              901 CAAGTCCGGA CTCAGATCTC GAGCTCAAGC TTCGAATTCT GCAGTCGACG GTACCGCGGG
              961 CCCGGGGATC CACCGGATCT AGATAAGGCC GCTCGAGCAT GCATCTAGAG GGCCCTATTC
             1021 TATAGTGTCA CCTAAATGCT AGAGCTCGCT GATCAGCCCT CGACTGTGCC TTCTAGTTTG
             1081 CCAGCCATCC TGCTGCTTGG CCCCTCCCCC GAGCCCGTTC CTTGGAGCCT GGAAGGGTGG
            1141 GC
            //
            Primer: SV40pro_F_V2 (Pr0154)
            Region: SV40 pro_ori, NeoR
            Sequence file: RDB13312_A5Dkb.seq check
            >06071_13312_A5Dk_SV40pro_F_V2_B06_06.ab1
                1 TGAAACTTTT TTGGAGGCTA GGCTTTTGCA AAAGCTCCCG GGAGCTTGGA TATCCATTTT
               61 CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA
              121 CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC
              181 AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT
              241 TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC
              301 GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG
              361 AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC
              421 TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC
              481 GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT
              541 GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC
              601 CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA
              661 TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA
              721 CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT
              781 TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC
              841 TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT
              901 CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC
              961 ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TTCCGGGACG CCCGGCTGGA
             1021 TGATCCTCCC AGCGCGGGGA TCTCATGCTG CAGTTCTTTC GCCCCACCCC ACTTGTTTAT
             1081 TGCAGCTTTA TATTGGTACG ATTAGGCCAT AGCCATCACA AAAGTTTCAC AATTAAAAGG
            1141 CCAA
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            2023.05.22

            GNP_filter3_RDBDEP_html_230512.pl