Resource data sheet
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pCMFlag_EGFP

Expression vector of GFP, with FLAG-tag, control vector.

Catalog number RDB06071
Resource name pCMFlag_EGFP
Alternative name SET-0108_4
Clone info. Expression vector of EGFP. Expression was confirmed by Western blotting with anti-FLAG antibody.
PCR amplified from a GFP expression vector.
Vector backbone pCMV_S-FLAG (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Aequorea victoria GFP cDNA
Depositor DNA Bank, |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL in any publication.
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06071 pCMFlag_EGFP DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content GFP Resources (English text)
Featured content pCMV_tag and pRSV_tag assay-ready clones (English text)
Featured content pCMV_tag and pRSV_tag vector (English text)
Featured content pCMV_tag and pRSV_tag vector (Japanese text)

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13312_A5Gl.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB13312_A5Gla.seq
>06071_13312_A5Gl_CMV-Forward_F12_18.ab1
    1 TATACACTCC GCCCCATTGA CGCAAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
   61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC
  121 GACTCACTAT AGGGAGACCC AAGCTGAATT CACCATGGAC TACAAGGACG ATGACGATAA
  181 GGCGGCCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA
  241 GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC
  301 CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG
  361 GCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA
  421 CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC
  481 CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA
  541 CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT
  601 GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA
  661 GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA
  721 GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA
  781 CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA
  841 CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA
  901 CAAGTCCGGA CTCAGATCTC GAGCTCAAGC TTCGAATTCT GCAGTCGACG GTACCGCGGG
  961 CCCGGGATCC ACCGGATCTA GATAAGGCCG CTCGAGCATG CATCTAGAGG GCCCTATTCT
 1021 ATAGTGTCAC CTAAATGCTA GAGCTCGCTG ATCAGCCTCG ACTGTGCTTC TAGTGCCAGC
 1081 CCATCTGTTG TTTGCCCCTC CCCGTGCGTC TTTGACCCTG CAGTGCCACT CCCACTGTCG
1141 ATCCTATAAT GAGAATGCAT CGCATGCCTG GACTAGGAGT TCATTCAGAT TCTCG
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.10.22

GNP_filter3_RDBDEP_html_181012.pl