Sequencing and PCR primers for primer information.
Expression vector of GFP, with FLAG-tag, control vector.
| Catalog number | RDB06071 |
|---|---|
| Resource name | pCMFlag_EGFP |
| Alternative name | SET-0108_4 |
| Clone info. | Expression vector of EGFP. Expression was confirmed by Western blotting with anti-FLAG antibody. PCR amplified from a GFP expression vector. |
| Vector backbone | pCMV_S-FLAG (Plasmid) |
| Size of vector backbone | 5.5 kb |
| Selectable markers | Amp^r (E. coli), Neo^r (mammalian cell) |
| Gene/insert name | Aequorea victoria GFP cDNA |
| Depositor | DNA Bank, | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL in any publication. |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link] |
| Remarks | ((Additional form)) GFP-A Please use Schedule A (GFP-A) additionally. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB06071 | pCMFlag_EGFP | DNA solution |
Electronic file
Featured content
| Featured content | GFP Resources (English text) |
|---|---|
| Featured content | pCMV_tag and pRSV_tag assay-ready clones (English text) |
| Featured content | pCMV_tag and pRSV_tag vector (English text) |
| Featured content | pCMV_tag and pRSV_tag vector (Japanese text) |
References
User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB13312_A5Gl.pdf |
|---|
Nucleotide sequence of a portion of this resource (if available).
| Primer: CMV-Forward | RDB13312_A5Gla.seq |
|---|---|
|
>06071_13312_A5Gl_CMV-Forward_F12_18.ab1 1 TATACACTCC GCCCCATTGA CGCAAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT 61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC 121 GACTCACTAT AGGGAGACCC AAGCTGAATT CACCATGGAC TACAAGGACG ATGACGATAA 181 GGCGGCCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA 241 GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC 301 CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG 361 GCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA 421 CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC 481 CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA 541 CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT 601 GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA 661 GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA 721 GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA 781 CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA 841 CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA 901 CAAGTCCGGA CTCAGATCTC GAGCTCAAGC TTCGAATTCT GCAGTCGACG GTACCGCGGG 961 CCCGGGATCC ACCGGATCTA GATAAGGCCG CTCGAGCATG CATCTAGAGG GCCCTATTCT 1021 ATAGTGTCAC CTAAATGCTA GAGCTCGCTG ATCAGCCTCG ACTGTGCTTC TAGTGCCAGC 1081 CCATCTGTTG TTTGCCCCTC CCCGTGCGTC TTTGACCCTG CAGTGCCACT CCCACTGTCG 1141 ATCCTATAAT GAGAATGCAT CGCATGCCTG GACTAGGAGT TCATTCAGAT TCTCG // |
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Please visit Sequencing and PCR primers for primer information.
References
2018.10.22
GNP_filter3_RDBDEP_html_181012.pl