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pCMFlag_hsTHRB

Expression vector of human TR beta (ErbA-beta).

Catalog number RDB06070
Resource name pCMFlag_hsTHRB
Alternative name SET-0102_33
Clone info. Expression vector of human TR beta (ErbA-beta). Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment PCR template:HEK293 cells cDNA. dT,Random primer mix. PCR cloning, forward primer: atgactcccaacagtatgacag; reverse primer: ctaatcctcgaacacttccaag. Known difference : C to T substitution at 543nt of NM_000461.2 (Glu, synonym).
Vector backbone pCMV_S-FLAG (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human TR beta; ErbA-beta cDNA
Depositor|Developer DNA Bank, |
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External Database
Human TR beta; ErbA-beta

          Reference sequence
            

          Distribution information

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          RDB06070 pCMFlag_hsTHRB DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence (full) RDB06070zzt01.seq compiled by us
          Remarks, protocol and/or map (pdf) RDB06070.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content pCMV_tag and pRSV_tag assay-ready clones (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB06070_07Gcp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro,FLAG,insert 5'
          Sequence file: RDB06070_07Gca.seq check
          >06070_06070_B1AjF_CMV-Forward_A03_03_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNA NNNNNGGTAG GCGTGTACGG TGGGAGGTCT
             61 ATATAAGCAG AGCTCTCTGG CTAACTAGAG AACCCACTGC TTACTGGCTT ATCGAAATTA
            121 ATACGACTCA CTATAGGGAG ACCCAAGCTG AATTCACCAT GGACTACAAG GACGATGACG
            181 ATAAGGCGGC CATGACTCCC AACAGTATGA CAGAAAATGG CCTTACAGCC TGGGACAAAC
            241 CGAAGCACTG TCCAGACCGA GAACACGACT GGAAGCTAGT AGGAATGTCT GAAGCCTGCC
            301 TACATAGGAA GAGCCATTCA GAGAGGCGCA GCACGTTGAA AAATGAACAG TCGTCGCCAC
            361 ATCTCATCCA GACCACTTGG ACTAGCTCAA TATTCCATCT GGACCATGAT GATGTGAACG
            421 ACCAGAGTGT CTCAAGTGCC CAGACCTTTC AAACGGAGGA GAAGAAATGT AAAGGGTACA
            481 TCCCCAGTTA CTTAGACAAG GACGAGCTCT GTGTAGTGTG TGGTGACAAA GCCACCGGGT
            541 ATCACTACCG CTGTATCACG TGTGAAGGCT GCNAGGGTTT CTTTAGAAGA ACCNTTCNGA
            601 AAAATCTCCA TCCNTCCTAT TCCTGTAAAT ATGAAGGAAA ATGTGTCNTA NACAAAGTCN
            661 CGCNAAATCN GTGNCNGNAA TGNCNCTTTN ANAANNGCNT CTATGTNGNN NTNGNNNNCN
          721 NANTTNGNGC TNGNNNN
          //
          Primer: bGH_rev3
          Region: bGHpA,insert 3'
          Sequence file: RDB06070_07Gcb.seq check
          >06070_06070_B1AjF_bGH_rev3_B11_05_ABI24.ab1
              1 NNNNNNNNNN NNNNNNGGNN GNNGGGCAAA CAACAGATGG CTGGCAANCT AGAAGGCACA
             61 GTCGAGGCTG ATCAGCGAGC TCTAGCATTT AGGTGACACT ATAGAATAGG GCCCTCTAGA
            121 TGCATGCTCG AGCGGCCCTA ATCCTCGAAC ACTTCCAAGA ACAAAGGGGG GAAGAGTTCT
            181 GTGGGGCATT CCACCTTCAT GTGCAGGAAG CGGCTGGCAT GGCAGGCTCC TATCATCCGC
            241 AGATCTGTCA CCTTCATCAG GAGTTTTGGC CAAAAGTGTG TCACGTGGTG TTTTCGGTAA
            301 TTGATATAGT GTTCAAAGGC CAGCAGGAAA CTATCTTGGT ACTTTTCTAT TCTCTCAACA
            361 CAGGCAAGCC CCGGGCGATC TGAAGACATC AGCAGGACGG CCTGAAGGAG GGCTACTTCA
            421 GTGTCATCCA GGTTGAAAGA AGACAGAGAC ATGCCCAGGT CAAAGATGGC GTCTGACACC
            481 ACCCCAAGAC CCCCATTTTT CAGCTGGCCC CGTGTCACTG CCATTTCCCC ATTCAAGGTT
            541 AAAGTCTCAC TTTCTGGGTC ATAGCGCACA GCAGCGCGAA NGGACATGAT CTCCATGCAG
            601 CAGCCTTTGA NGANGANGAT CTGGTCTTCA CATGGCAGCT CACNAAACAT ANGNNACTTT
            661 TTGGNNAAAT CCACCACTCN NGTAANNGCT GGNGTGANGA NTTTNNNAAA ATNNNNNNNN
          721 NN
          //
          Primer: SV40pro_ori_F
          Region: SV40 pro_ori,NeoR
          Sequence file: RDB06070_07Gcc.seq check
          >06070_06070_B1AjF_SV40pro_ori_F_B12_06_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NCNGCCNNNN NNCTCTGNNC TNTTCNNGAN NNNNNTGNNG
             61 GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGG ATATCCATTT
            121 TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC
            181 ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA
            241 CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT
            301 TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT
            361 CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG
            421 GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG
            481 CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC
            541 CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCNCAT CGAGCGNNCA CGTACTCNGA
            601 TGGNANCCCG GTCTTGNCNA TCANGATGAT CTGGANNANN ANCATNNNNG CTCGCGCCNG
            661 CCGANNNGNN CGCNNGGCTN NNNGCGCGCA TGCCCNANNN GAGNNCNCGN CNNGANCCNN
            721 GNNNNNGCCN GNNTGNCNAA TNNCNNNGGT NNNNAANNNN NNNNTTNNNN GNNNNNNNNN
          781 N
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl