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pCMFlag_hsTHRB (#RDB06070)

Expression vector of human TR beta (ErbA-beta).

Alternative name SET-0102_33
Clone info. Expression vector of human TR beta (ErbA-beta). Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment PCR template：HEK293 cells cDNA. dT,Random primer mix. PCR cloning, forward primer: atgactcccaacagtatgacag; reverse primer: ctaatcctcgaacacttccaag. Known difference : C to T substitution at 543nt of NM_000461.2 (Glu, synonym).
Vector backbone pCMV_S-FLAG (RDB05956) (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human TR beta; ErbA-beta cDNA
Depositor|Developer DNA Bank, |
 
Sequence (full) RDB06070zzt01.seq compiled by us
Remarks, protocol and/or map (pdf) RDB06070.pdf

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RDB06070 pCMFlag_hsTHRB DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMFlag_hsTHRB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06070).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB06070_07Gcp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,FLAG,insert 5'
Sequence file: RDB06070_07Gca.seq check
>06070_06070_B1AjF_CMV-Forward_A03_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNA NNNNNGGTAG GCGTGTACGG TGGGAGGTCT
   61 ATATAAGCAG AGCTCTCTGG CTAACTAGAG AACCCACTGC TTACTGGCTT ATCGAAATTA
  121 ATACGACTCA CTATAGGGAG ACCCAAGCTG AATTCACCAT GGACTACAAG GACGATGACG
  181 ATAAGGCGGC CATGACTCCC AACAGTATGA CAGAAAATGG CCTTACAGCC TGGGACAAAC
  241 CGAAGCACTG TCCAGACCGA GAACACGACT GGAAGCTAGT AGGAATGTCT GAAGCCTGCC
  301 TACATAGGAA GAGCCATTCA GAGAGGCGCA GCACGTTGAA AAATGAACAG TCGTCGCCAC
  361 ATCTCATCCA GACCACTTGG ACTAGCTCAA TATTCCATCT GGACCATGAT GATGTGAACG
  421 ACCAGAGTGT CTCAAGTGCC CAGACCTTTC AAACGGAGGA GAAGAAATGT AAAGGGTACA
  481 TCCCCAGTTA CTTAGACAAG GACGAGCTCT GTGTAGTGTG TGGTGACAAA GCCACCGGGT
  541 ATCACTACCG CTGTATCACG TGTGAAGGCT GCNAGGGTTT CTTTAGAAGA ACCNTTCNGA
  601 AAAATCTCCA TCCNTCCTAT TCCTGTAAAT ATGAAGGAAA ATGTGTCNTA NACAAAGTCN
  661 CGCNAAATCN GTGNCNGNAA TGNCNCTTTN ANAANNGCNT CTATGTNGNN NTNGNNNNCN
721 NANTTNGNGC TNGNNNN
//
Primer: bGH_rev3 (Pr0885)
Region: bGHpA,insert 3'
Sequence file: RDB06070_07Gcb.seq check
>06070_06070_B1AjF_bGH_rev3_B11_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGGNN GNNGGGCAAA CAACAGATGG CTGGCAANCT AGAAGGCACA
   61 GTCGAGGCTG ATCAGCGAGC TCTAGCATTT AGGTGACACT ATAGAATAGG GCCCTCTAGA
  121 TGCATGCTCG AGCGGCCCTA ATCCTCGAAC ACTTCCAAGA ACAAAGGGGG GAAGAGTTCT
  181 GTGGGGCATT CCACCTTCAT GTGCAGGAAG CGGCTGGCAT GGCAGGCTCC TATCATCCGC
  241 AGATCTGTCA CCTTCATCAG GAGTTTTGGC CAAAAGTGTG TCACGTGGTG TTTTCGGTAA
  301 TTGATATAGT GTTCAAAGGC CAGCAGGAAA CTATCTTGGT ACTTTTCTAT TCTCTCAACA
  361 CAGGCAAGCC CCGGGCGATC TGAAGACATC AGCAGGACGG CCTGAAGGAG GGCTACTTCA
  421 GTGTCATCCA GGTTGAAAGA AGACAGAGAC ATGCCCAGGT CAAAGATGGC GTCTGACACC
  481 ACCCCAAGAC CCCCATTTTT CAGCTGGCCC CGTGTCACTG CCATTTCCCC ATTCAAGGTT
  541 AAAGTCTCAC TTTCTGGGTC ATAGCGCACA GCAGCGCGAA NGGACATGAT CTCCATGCAG
  601 CAGCCTTTGA NGANGANGAT CTGGTCTTCA CATGGCAGCT CACNAAACAT ANGNNACTTT
  661 TTGGNNAAAT CCACCACTCN NGTAANNGCT GGNGTGANGA NTTTNNNAAA ATNNNNNNNN
721 NN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori,NeoR
Sequence file: RDB06070_07Gcc.seq check
>06070_06070_B1AjF_SV40pro_ori_F_B12_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NCNGCCNNNN NNCTCTGNNC TNTTCNNGAN NNNNNTGNNG
   61 GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGG ATATCCATTT
  121 TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC
  181 ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA
  241 CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT
  301 TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT
  361 CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG
  421 GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG
  481 CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC
  541 CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCNCAT CGAGCGNNCA CGTACTCNGA
  601 TGGNANCCCG GTCTTGNCNA TCANGATGAT CTGGANNANN ANCATNNNNG CTCGCGCCNG
  661 CCGANNNGNN CGCNNGGCTN NNNGCGCGCA TGCCCNANNN GAGNNCNCGN CNNGANCCNN
  721 GNNNNNGCCN GNNTGNCNAA TNNCNNNGGT NNNNAANNNN NNNNTTNNNN GNNNNNNNNN
781 N
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles