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pCMFlag_hsPPARD (#RDB06068)

Expression vector of human PPARD/PPAR delta.

Alternative name SET-0082_5
Clone info. Expression vector of human PPARD/PPAR delta. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment PCR template：HEK293 cells cDNA. dT,Random primer mix. PCR cloning, forward primer: gagcagccacaggaggaagcccct; reverse primer:ggaggcctgggtgccgccgttag.
Vector backbone pCMV_S-FLAG (RDB05956) (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human PPARD/PPAR delta cDNA
Depositor|Developer DNA Bank, |
 
Sequence RDB06068z.seq
Remarks, protocol and/or map (pdf) RDB06068.pdf

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RDB06068 pCMFlag_hsPPARD DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMFlag_hsPPARD was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06068).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13528_A5Ipp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40
Sequence file: RDB13528_A5Ipa.seq check
>06068_13528_A5Ip_CMV-Forward_C11_08.ab1
    1 TTATTCAACT CGCCCCATTG ACGCAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
   61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC
  121 GACTCACTAT AGGGAGACCC AAGCTGAATT CACCATGGAC TACAAGGACG ATGACGATAA
  181 GGCGGCCGAG CAGCCACAGG AGGAAGCCCC TGAGGTCCGG GAAGAGGAGG AGAAAGAGGA
  241 AGTGGCAGAG GCAGAAGGAG CCCCAGAGCT CAATGGGGGA CCACAGCATG CACTTCCTTC
  301 CAGCAGCTAC ACAGACCTCT CCCGGAGCTC CTCGCCACCC TCACTGCTGG ACCAACTGCA
  361 GATGGGCTGT GACGGGGCCT CATGCGGCAG CCTCAACATG GAGTGCCGGG TGTGCGGGGA
  421 CAAGGCATCG GGCTTCCACT ACGGTGTTCA TGCATGTGAG GGGTGCAAGG GCTTCTTCCG
  481 TCGTACGATC CGCATGAAGC TGGAGTACGA GAAGTGTGAG CGCAGCTGCA AGATTCAGAA
  541 GAAGAACCGC AACAAGTGCC AGTACTGCCG CTTCCAGAAG TGCCTGGCAC TGGGCATGTC
  601 ACACAACGCT ATCCGTTTTG GTCGGATGCC GGAGGCTGAG AAGAGGAAGC TGGTGGCAGG
  661 GCTGACTGCA AACGAGGGGA GCCAGTACAA CCCACAGGTG GCCGACCTGA AGGCCTTCTC
  721 CAAGCACATC TACAATGCCT ACCTGAAAAA CTTCAACATG ACCAAAAAGA AGGCCCGCAG
  781 CATCCTCACC GGCAAAGCCA GCCACACGGC GCCCTTTGTG ATCCACGACA TCGAGACATT
  841 GTGGCAGGCA GAGAAGGGGC TGGTGTGGAA GCAGTTGGTG AATGGCCTGC CTCCCTACAA
  901 GGAGATCAGC GTGCACGTCT TCTACCGCTG CCAGTGCACC ACAGTGGAGA CCGTGCGGGA
  961 GCTCACTGAG TTCGCCAAGA GCATCCCCAG CTTTCAGCAG CCTCTTCCTC AACGACCAGG
 1021 GTTACCCTTC TCAAGTATGG CGTGCACGAG CCATCTTCGC ATGCTGGCTC TATCGTCAAC
 1081 AGAACGGCTG CTGTAGCCAA CGCAGTTGCT TTGTCACCGG TGGAGTTCCT GGCCAGCCTC
1141 GGCAAACCAT TCAGGTGAAA TATC
//
Primer: Reverse2
Sequence file: RDB13528_A5Ipb.seq check
>06068_13528_A5Ip_BGHrev_E02_14.ab1
    1 GGCTTGGCAT TTAGGTGACC TATAGATAGG GCCCTCTAGA TGCATGCTCG AGCGGCCGGA
   61 GGCCTGGGTG CCGCCGTTAG TACATGTCCT TGTAGATCTC CTGGAGCAGA GGGTGCAGCG
  121 AGGTCTCGGT TTCGGTCTTC TTGATCCGCT GCATCATCTG GGCGTGCTCG GTGACCAGTT
  181 GCCGCAGGTC AGCCATCTTC TGCAGCAGCT TGGGGAAGAG GTACTGGGCA TCAGGGTGGT
  241 TGGCCTGCAG GTGGAATTCG AGGGCACGCA GGATGGTGTC CTGGATAGCC TCCACCCGTG
  301 GAACGTTCAT GAGGCCTGGC CGGTCTCCAC ACAGAATGAT GGCCGCAATG AATAGGGCCA
  361 GGTCACTGTC ATCAAGTTCC AGGGCGTTGA ACTTGACAGC AAATTCAAAC TTAGGCTCAA
  421 TGATATCACT GAAGGGTTTG CGGAGGCTGC GCAGGAACTC ACGGGTGACA AAGCCACTGC
  481 CGTTGGCTAC CAGCAGCCCG TCCTTGTTGA CGATAGAGGC CAGCATGGCG AAGATGGCCT
  541 CGTGCACGCC ATACTTGAGA AGGGTAACCT GGTCGTTGAG GAAGAGGCTG CTGAAGCTGG
  601 GGATGCTCTT GGCGAACTCA GTGAGCTCCC GCACGGTCTC CACTGTGGTG CACTGGCAGC
  661 GGTAGAAGAC GTGCACGCTG ATCTCCTTGT AGGGAGGCAG GCCATTCACC AACTGCTTCC
  721 ACACCAGCCC CTTCTCTGCC TGCCACAATG TCTCGATGTC GTGGATCACA AAGGGCGCCG
  781 TGTGGCTGGC TTTGCCGGTG AGGATGCTGC GGGCCTTCTT TTTGGTCATG TTGAAGTTTT
  841 TCAGGTAGGC ATTGTAGATG TGCTTGGAGA AGGCCTTCAG GTCGGCCACC TGTGGGTTGT
  901 ACTGGCTCCC CTCGTTTGCA GTCAGCCCTG CCACCAGCTT CCTCTTCTCA GCCTCCGGCA
  961 TCCGACCAAA ACGGATAGCG TTGTGTGACA TGCCCAGTGC CAGGCACTTC TGGAAGCGGC
 1021 AGTACTGGGC ACTTGTTTGC GGGTTCTTTC TTCTGAATTC TTGCAGCTGC GCTCACACTT
 1081 TCTTCGTACT CCAGCTTCAT GCGGGATCGG TTACGAACGG AGGAAGCCCT TGCACCCCCC
1141 TCAACATGGC AATGCAACAC ACCCGGTTAG ATTGTGGA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles