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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

hTSHR (#RDB06008)

Expression vector of human TSHR

Clone info. Expression vector of human thyrotropin receptor (TSHR). Inserted fragment is opposite direction to the promoter.
Vector backbone pSG5 (Plasmid)
Selectable markers Amp^r
Gene/insert name Human TSHR cDNA
Depositor|Developer Tahara, Kazuo | Tahara, Kazuo |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Biochem Biophys Res Commun., 179, 70-77 (1991)).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Biochem Biophys Res Commun., 179, 70-77 (1991)).
備考 提供条件の言語は英語のみ。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06008 hTSHR DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The hTSHR was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06008).

Reference section:

Tahara, K., Ban, T., Minegishi, T., Kohn, L.D., Immunoglobulins from Graves' disease patients interact with different sites on TSH receptor/LH-CG receptor chimeras than either TSH or immunoglobulins from idiopathic myxedema patients Biochem Biophys Res Commun., 179, 70-77 (1991). PMID 1883391. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB20089_B3Ilp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: beta-gi_F (Pr0497)
Region: T7 pro, insert 3'
Sequence file: RDB20089_B3Ila.seq check
>06008_20089_B3Il_A2_beta-gi_F_B05_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNTNNT TTTNNNNACN GCTCCTGGGC AACGTGCTGG TTATTGTGCT
   61 GTCTCATCAN TNNNNNNAAG AATTGTAATA CGACTCACTA TAGGGCGAAT TCCGGTTGGG
  121 GGTGTCATGG GATTGGAATG CATATTCAAG AAACTATTAT TTTGTAAGTT CCCCTACCAT
  181 TGTGAGTAGT GTAGTGTTAA CTTACAAAAC CGTTTGCATA TACTCTTCTG AGATTTGGCC
  241 TTGCTTCTTT GGGGTTAGAT GGGAGTTTTC AATCAGTTCA TAGACATCTT CCATGTTGTG
  301 GAGACCCTGC CTCATGTCGT GGGTAACCTT TTGAACCTGA ATATCAGTGC TGTTCTTTGG
  361 AGGAACCCTC TGCCCCCGGT ATGCCTGAGC CTGGCGTTTA CAGATGCCAA ACTTGCTGAG
  421 TAGGATGAAC ACATCCCTCT GGAAGGCCTT GGTGAAAATA GCATAGAGGA ATGGATTGGC
  481 ACAGGAGTTA AGTGGATAGA AGAGTACCAG CAAGATTTTG GAGTTGCTAA CAGTGATGAG
  541 AGGCTTGTTC AGAATTGCTG ACAGAGCATA GAATGAGATT GGGGCCATGC ATATGAAGTC
  601 GGTGAAGATC AACACAGCCA TCCTCTTGGC AATTTTGGTA TCTTTGTCCC CTGGGTTGTA
  661 CTGCGGATTT CGGACTGTGA TGTAGATCTT CACATAACAG CAGCAGACGA TGACGAANGC
  721 AACTATGTTG AGCGTCAGAA CAAAAACNAT ATATGCCAGA GCAAGANGGG TCTCGGTGTC
  781 CATGGGCAGG CAGATACTGA CTTTGGCATA NCTACTTATT CCCACCAAAG GAAGCANGGC
  841 GAGAANGAAN CANCAAANCC AGCCCCCNNN NNNGATGGNA CATGCNNGCC TNANGNNGAN
  901 CTNNCNGNNC NNNCGCATGG NNNANNNGAN NNNATANNNN CGCTNCNNGG NGNNGNNNNN
961 NNNNN
//
Primer: pAxEF R2 (Pr0059)
Region: insert 5'
Sequence file: RDB20089_B3Ilb.seq check
>06008_20089_B3Il_A2_pAxEF R2_C05_03_ABI08.ab1
    1 NNNNNNNNNN NNNTTNNNTT GNGNANNTTG TGNNGCTATT GCTTTATTTG TAACCATTAT
   61 AAGCTGCAAN NNNNNNNNTC TGCTTTAATA AGATCTGGAT CCGAATTCCG GCNNAGCTGA
  121 GAATGAGGCG ATTTCGGAGG ATGGAGAAAT AGCCCCGAGT CCCGTGGAAA ATGAGGCCGG
  181 CGGACTTGCT GCAGCTGGTG CTGCTGCTCG ACCTGCCCAG GGACCTGGGC GGAATGGGGT
  241 GTTCGTCTCC ACCCTGCGAG TGCCATCAGG AGGAGGACTT CAGAGTCACC TGCAAGGATA
  301 TTCAACGCAT CCCCAGCTTA CCGCCCAGTA CGCAGACTCT GAAGCTTATT GAGACTCACC
  361 TGAGAACTAT TCCAAGTCAT GCATTTTCTA ATCTGCCCAA TATTTCCAGA ATCTACGTAT
  421 CTATAGATGT GACTCTGCAG CAGCTGGAAT CACACTCCTT CTACAATTTG AGTAAAGTGA
  481 CTCACATAGA AATTCGGAAT ACCAGGAACT TAACTTACAT AGACCCTGAT GCCCTCAAAG
  541 AGCTCCCCCT CCTAAAGTTC CTTGGCATTT TCAACACTGG ACTTAAAATG TTCCCTGACC
  601 TGACCAAAGT TTATTCCACT GATATATTCT TTATACTTGA AATTACAGAC AACCCTTACA
  661 TGACGTCAAT CCCTGTGAAT GCTTTTCAGG GACTATGCAA TGAAACCTTG ACACTGAAGC
  721 TGTACAACAA TGGCTTTACT TCAGTCCAAG GATATGCTTT CAATGGGACA AAGCTGGATG
  781 CTGTTTACCT AAACAAGANT AAATACCTGA NAGTTATTGA CAAAGATGCA TTTGGAGGAN
  841 TATACANNGN ANNNAGCTNG CNGGANGNNN CNCNNNNCAG NGTCNCTNNC CNTNCATCCN
901 ANNNNNNNNN N
//
Primer: SV40pro_ori_F (Pr0731)
Region: beta-globin intron,T7 pro
Sequence file: RDB20089_B3Ilc.seq check
>06008_20089_B3Il_A2_SV40pro_ori_F_D05_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNC NCCTCNNCCT CTGNNCTATT CCNGAAGTAG TGAAGAGGCT
   61 TTTTTGGAGN NCCTANGCTT TTGCAAAAAG CTCCGGATCG ATCCTGAGAA CTTCANGGTG
  121 AGTTTGGGGA CCCTTGATTG TTCTTTCTTT TTCGCTATTG TAAAATTCAT GTTATATGGA
  181 GGGGGCAAAG TTTTCAGGGT GTTGTTTAGA ATGGGAAGAT GTCCCTTGTA TCACCATGGA
  241 CCCTCATGAT AATTTTGTTT CTTTCACTTT CTACTCTGTT GACAACCATT GTCTCCTCTT
  301 ATTTTCTTTT CATTTTCTGT AACTTTTTCG TTAAACTTTA GCTTGCATTT GTAACGAATT
  361 TTTAAATTCA CTTTTGTTTA TTTGTCAGAT TGTAAGTACT TTCTCTAATC ACTTTTTTTT
  421 CAAGGCAATC AGGGTATATT ATATTGTACT TCAGCACAGT TTTAGAGAAC AATTGTTATA
  481 ATTAAATGAT AAGGTAGAAT ATTTCTGCAT ATAAATTCTG GCTGGCGTGG AAATATTCTT
  541 ATTGGTAGAA ACAACTACAT CCTGGTCATC ATCCTGCCTT TCTCTTTATG GTTACAATGA
  601 TATACACTGT TTGAGATGAG GATAAAATAC TCTGAGTCCA AACCGGGCCC CTCTGCTAAC
  661 CATGTTCATG CCTTCTTCTT TTTCCTACAG CTCCTGGGCA ACGTGCTGGT TATTGTGCTG
  721 TCTCATCATT TTGGCAAAGA ATTGTAATAC GACTCACTAT AGGGCGAATT CCGGTTGGGN
  781 NTGTCATGGG NNTGGANTGC ATATTCNAGA AACTATTATT TTGTAAGTTC CCNTACCATT
  841 GTGAGTAGTG NANNGTTAAC TTNCAAAANC NTTTGCATAT ACTCTTCTNA NATTNNGNCN
901 NGCTNNNTNG GGNNNANANG GGANTTTTNN NNCANTNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tahara, K., Immunoglobulins from Graves' disease patients interact with different sites on TSH receptor/LH-CG receptor chimeras than either TSH or immunoglobulins from idiopathic myxedema patients Biochem Biophys Res Commun., 179, 70-77 (1991). PMID 1883391. [link to RRC of NBRP]