Please review the QC test results indicated by icon below as well as clone information before placing your order.

CS-CA-Venus

Lentivirus vector plasmid of Venus fluorescent protein driven by CAG promoter.

Sequence (full)	RDB05965zzk02.seq provided by the depositor
Remarks, protocol and/or map (pdf)	RDB05965.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Please visit Ordering instruction.[link]
Remarks	((Remarks)) Remember that you will be working with samples containing infectious virus. Please contact us. We will provide you with appropriate documents.

提供案内 (日本国内) [open/close]

提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
備考	【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16933_A9Blp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CAG_R1 (Pr0584) Region: CAG pro,Venus 5' Sequence file: RDB16933_A9Bla.seq
>05965_16933_B2BfF_CAG_R1_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNTNNNNNN GGCGCGGGGC GNAGCGGTGC GGCGCCGGCA    61 GGAAGGAAAT GGGCGGGGAG GGCCTTCGTG CGTCGCCGCG CCGCCGTCCC CTTCTCCCTC   121 TCCAGCCTCG GGGCTGTCCG CGGGGGGACG GCTGCCTTCG GGGGGGACGG GGCAGGGCGG   181 GGTTCGGCTT CTGGCGTGTG ACCGGCGGCT CTAGAGCCTC TGCTAACCAT GTTCATGCCT   241 TCTTCTTTTT CCTACAGCTC CTGGGCAACG TGCTGGTTAT TGTGCTGTCT CATCATTTTG   301 GCAAAGAATT GATTTGATAC GTACGGTTGG GCTGCAGGAA TTGATCCACC ATGGTGAGCA   361 AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA   421 ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA   481 CCCTGAAGCT GATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA   541 CCCTGGGCTA CGGCCTGCAG TGCTTCGCCC GCTACCCCGA CCACATGAAG CAGCACGACT   601 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG   661 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA   721 TCGAGCTGAA GGGCATCGAC TTCNAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT   781 ACAACTACAA CAGCCACAAC GTCTATATCA CCGCCGANNA GCAGAAGANC GGCATCAAGG   841 CCNACTTCNA GATCCGCCAC AACATCGNNG ANGGCGGCGT GCAGCTCGCC NANNNCTACC   901 NGCNNANCNC CNNCNTCGNC GANNGNCCCN NGCNGCTGNC CGANAACCAC TANCTGANCT   961 ANCNNTCCNN CCTNANCNAA GANCCNNACG AGAANCNNGN TNNNNTNGNN NNNGNTNGAN 1021 TTCNNNNNNN NNN //
Primer: PRE_rev (Pr0250) Region: PRE,Venus 3' Sequence file: RDB16933_A9Blb.seq
>05965_16933_B2BfF_PRE_rev_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNG NANNNNGAGC AACATAGTTN NNANTACCNG TCAATCTTTC    61 ACAAATTTTG TAATCCAGAG GTTGATTATC GATAAGCTTG ATCCCTCGAG AGGCCTTGAA   121 TTCTTACTTG TACAGCTCGT CCATGCCGAG AGTGATCCCG GCGGCGGTCA CGAACTCCAG   181 CAGGACCATG TGATCGCGCT TCTCGTTGGG GTCTTTGCTC AGGGCGGACT GGTAGCTCAG   241 GTAGTGGTTG TCGGGCAGCA GCACGGGGCC GTCGCCGATG GGGGTGTTCT GCTGGTAGTG   301 GTCGGCGAGC TGCACGCCGC CGTCCTCGAT GTTGTGGCGG ATCTTGAAGT TGGCCTTGAT   361 GCCGTTCTTC TGCTTGTCGG CGGTGATATA GACGTTGTGG CTGTTGTAGT TGTACTCCAG   421 CTTGTGCCCC AGGATGTTGC CGTCCTCCTT GAAGTCGATG CCCTTCAGCT CGATGCGGTT   481 CACCAGGGTG TCGCCCTCGA ACTTCACCTC GGCGCGGGTC TTGTAGTTGC CGTCGTCCTT   541 GAAGAAGATG GTGCGCTCCT GGACGTAGCC TTCGGGCATG GCGGACTTGA AGAAGTCGTG   601 CTGCTTCATG TGGTCGGGGT AGCGGGCGAA GCACTGCAGG CCGTAGCCCA GGGTGGTCAC   661 GAGGGTGGGC CAGGGCACGG GCAGCTTGCC GGTGGTGCAG ATCAGCTTCA GGGTCAGCTT   721 GCCGTANGTG GCATCGCCCT CGCCCTCGCC GGACACGCTG AACTTGTGGN CGTTTACGTC   781 GCCGTCCAGC TCGACNAGGA TGGGCACCAC CCCGGTGAAC AGCTCCTCGC CCTTGCTCAC   841 CATGGTGGNT CNATTCCTGC AGCCCNACCG TACGTATCNA ATCNATTCTT TGCCNAAATG   901 ATGANACAGC ACAATNNCCN GCACGTNGNC NNNGANCTGT ANGAAAAANA ANAANGNATG   961 AANNTNGTTN GCAGANGNTC TANANCCGNC NNNNNCNNNN NNNANNNCNG NNNCNCGCCN 1021 NNNCCNNNNC CCNNCCNNNN NNNNNN //
Primer: CMV_Forward (Pr0016) Region: CMV pro,5'LTR,HIV-1 psi Sequence file: RDB16933_A9Blc.seq
>05965_16933_A9Bl_CMV_Forward_G01_07_ABI08.ab1     1 NNNNNNNNNN NNNNCCCNNT TGNNGCNNNN GGGCGGNAGG CGTGTACGGT GGGAGGTCTA    61 TATAAGCAGC GCGTTTTGCC TGTACTGGGT CTCTCTGGTT AGACCAGATC TGAGCCTGGG   121 AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA ATAAAGCTTG CCTTGAGTGC   181 TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA CTAGAGATCC CTCAGACCCT   241 TTTAGTCAGT GTGGAAAATC TCTAGCAGTG GCGCCCGAAC AGGGACTTGA AAGCGAAAGG   301 GAAACCAGAG GAGCTCTCTC GACGCAGGAC TCGGCTTGCT GAAGCGCGCA CGGCAAGAGG   361 CGAGGGGCGG CGACTGGTGA GTACGCCAAA AATTTTGACT AGCGGAGGCT AGAAGGAGAG   421 AGATGGGTGC GAGAGCGTCA GTATTAAGCG GGGGAGAATT AGATCGCGAT GGGAAAAAAT   481 TCGGTTAAGG CCAGGGGGAA AGAAAAAATA TAAATTAAAA CATATAGTAT GGGCAAGCAG   541 GGAGCTAGAA CGATTCGCAG TTAATCCTGG CCTGTTAGAA ACATCAGAAG GCTGTAGACA   601 AATACTGGGA CAGCTACAAC CATCCCTTCA GACAGGATCA GAAGAACTTA GATCATTATA   661 TAATACAGTA GCAACCCTCT ATTGTGTGCA TCAAAGGATA GAGATAAAAG ACACCAAGGA   721 AGCTTTAGAC AAGATAGAGG AAGAGCAAAA CAAAAGTAAG ACCACCGCAC AGCAAGCGGC   781 CGCTGATCTT CAGACCTGGA GGAGGAGATA TGANGGANAA TTGGAGAAGT GANTTATATA   841 AATATAAAGT AGTAAAAATT GNNCCNTTAN GAGTANCACC CACCNAGGCA AAGAGAAGAG   901 TGGTGCAGAG AGAAAAAAGA NCANNGGGAN TAGGAGCTTT GTTCCNTGGG TTCTTGGGAN 961 CNNCNNNNGC ANTATGGGGC GCNNNNNCNA TGNNNCTGNN GGNANNNNNN NNNN //
Primer: RRE_F1 (Pr0796) Region: RRE Sequence file: RDB16933_A9Bld.seq
>05965_16933_B2BfF_RRE_F1_G01_07_ABI08.ab1     1 NNNNNNNNNN NNNNNCNNNN NNNNNNNNTG CTGANGGCTA TTGAGGCGCA ACAGCATCTG    61 TTGCAACTCA CAGTCTGGGG CATCAAGCAG CTCCAGGCAA GAATCCTGGC TGTGGAAAGA   121 TACCTAAAGG ATCAACAGCT CCTGGGGATT TGGGGTTGCT CTGGAAAACT CATTTGCACC   181 ACTGCTGTGC CTTGGAATGC TAGTTGGAGT AATAAATCTC TGGAACAGAT TTGGAATCAC   241 ACGACCTGGA TGGAGTGGGA CAGAGAAATT AACAATTACA CAAGCTTAAT ACACTCCTTA   301 ATTGAAGAAT CGCAAAACCA GCAAGAAAAG AATGAACAAG AATTATTGGA ATTAGATAAA   361 TGGGCAAGTT TGTGGAATTG GTTTAACATA ACAAATTGGC TGTGGTATAT AAAATTATTC   421 ATAATGATAG TAGGAGGCTT GGTAGGTTTA AGAATAGTTT TTGCTGTACT TTCTATAGTG   481 AATAGAGTTA GGCAGGGATA TTCACCATTA TCGTTTCAGA CCCACCTCCC AACCCCGAGG   541 GGACCCGACA GGCCCGAAGG AATAGAAGAA GAAGGTGGAG AGAGAGACAG AGACAGATCC   601 ATTCGATTAN TGAACGGNTC TACNAATGGC AGTATTCATC CNCNATTTTA AAAGAAAAGG   661 GGGGNNTGGG GGGGNACAGN GCNNGGGNNN NNAGTAGACN TNANNNCNNN NGANNTNNNA 721 NCTNANNNAT TNCNAANNNA NNNNNNNAAA ANNNNNAANN TTTNNN //
Primer: env_F (Pr0258) Region: cPPT_CTS,CAG pro Sequence file: RDB16933_A9Ble.seq
>05965_16993_A9Bl_1_env_F_D10_10_ABI24.ab1     1 CTACNAATGG CAGTATTCAT CCNCAATTTT AAAAGAAAAG GGGGGATTGG GGGGTACAGT    61 GCAGGGGAAA GAATAGTAGA CATAATAGCA ACAGACATAC AAACTAAAGA ATTACAAAAA   121 CAAATTACAA AAATTCAAAA TTTTCGGGTT TATTACAGGG ACAGCAGAAA TTCACTTTGA   181 ATTGGGCTGC AGGAATCTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC   241 CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC   301 AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG   361 ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT   421 CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC   481 TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA   541 TTAGTCATCG CTATTACCAT GGTCGAGGTG AGCCCCACGT TCTGCTTCAC TCTCCCCATC   601 TCCCCCCCCT CCCCACCCCC AATTTTGTAT TTATTTATTT TTTAATTATT TTGTGCAGCG 661 ATGGGGGCGG GGGGGGGGGG G //
Primer: bGH_rev3 (Pr0885) Region: bGH pA,3'LTR Sequence file: RDB16933_A9Blf.seq
>05965_16933_B2BfF_bGH_rev3_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG ATNGGCTGGC AACTAGAAGG    61 CACAGTCGAG GCTGATCNNC GGGTTTAAAC GGGCCCTGCT AGAGATTTTC CACACTGACT   121 AAAAGGGTCT GAGGGATCTC TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT   181 GAAGCACTCA AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA   241 GCTCCCAGGC TCAGATCTGG TCTAACCAGA GAGACCCAGT ACAGTCCGGA TGCAGCTCTC   301 GGGCCATGTG ATGAAATGCT AGGCGGCTGT CAAACCTCCA CTCTAATACT TCTCTCTCCG   361 GGTCATCCAT CCCATGCAGG CTCACAGGGT GTAACAAGCG GGTGTTCTCT CCTTCATTGG   421 CTTCTTCTAC CTTCTCTTGC TCAACTGGTA CTAGCTTGTA GCACCATCCA AAGGTCAGTG   481 GATATCTGAT CCCTGGCCCT GGTGTGTAGT TCTGCCAATC AGGGAAGTAG CCTTGTGTGT   541 GGTAGATCCA CAGATCAAGG ATATCTTGTC TTCGTTGGGA GTGAATTAGC CCTTCCAGTC   601 CCCCCTTTTC NTTTAAAAAG TGGCTAANAN CTACAGCTGC CTTGTAAGTC ATTGGTCTTA   661 AANGNACCTG NNNGTGANTG GAAAANCCNN CTCCTCCTCC TCTNGNGCTT CNNNCCNGGC 721 NCNATCAGCN NNNNNNNNN //
Primer: SV40pro_F (Pr0110) Region: SV40 pro_ori,Zeo,SV40 pA Sequence file: RDB16933_A9Blg.seq
>05965_16993_A9Bl_1_SV40pro_F_E10_13_ABI24.ab1     1 GTGAGGANGN TTTTTTGGAG GNCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC    61 CATTTTCGGA TCTGATCAGC ACGTGTTGAC AATTAATCAT CGGCATAGTA TATCGGCATA   121 GTATAATACG ACAAGGTGAG GAACTAAACC ATGGCCAAGT TGACCAGTGC CGTTCCGGTG   181 CTCACCGCGC GCGACGTCGC CGGAGCGGTC GAGTTCTGGA CCGACCGGCT CGGGTTCTCC   241 CGGGACTTCG TGGAGGACGA CTTCGCCGGT GTGGTCCGGG ACGACGTGAC CCTGTTCATC   301 AGCGCGGTCC AGGACCAGGT GGTGCCGGAC AACACCCTGG CCTGGGTGTG GGTGCGCGGC   361 CTGGACGAGC TGTACGCCGA GTGGTCGGAG GTCGTGTCCA CGAACTTCCG GGACGCCTCC   421 GGGCCGGCCA TGACCGAGAT CGGCGAGCAG CCGTGGGGGC GGGAGTTCGC CCTGCGCGAC   481 CCGGCCGGCA ACTGCGTGCA CTTCGTGGCC GAGGAGCAGG ACTGACACGT GCTACGAGAT   541 TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC   601 GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG   661 TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA   721 GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT   781 GTCTGTATAC CGTCGANCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT   841 GTGTGAAATT GTTATCCGCT CACNATTCCA CACNACATAC GAGCCGGAAG CATNAAGTGT   901 AAAGNCNGGG GNGNCNNATG ANTGANCTAN NTCANNTTAA TTGCNTTGCN CTCACTGCCC 961 NCTTTNCAG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.11.23

GNP_filter3_RDBDEP_html_231027.pl