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CS-CA-Venus

Lentivirus vector plasmid of Venus fluorescent protein driven by CAG promoter.

Catalog number RDB05965
Resource name CS-CA-Venus
Clone info. Lentivirus vector plasmid of Venus fluorescent protein driven by CAG promoter.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CS-CA-MCS (Plasmid)
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Miyoshi, Hiroyuki |
Other clones in our bank

External Database
Aequorea victoria GFP

            Reference sequence
              
             
            Sequence (full) RDB05965zzk02.seq provided by the depositor
            Remarks, protocol and/or map (pdf) RDB05965.pdf
            Lentiviral plasmid list. [link]

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
            Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding Venus fluorescent protein.
            Ordering Please visit Ordering instruction.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
            Please contact us. We will provide you with appropriate documents.
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
            付加的提供条件 利用者は、Venus蛍光タンパク質について理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。
            提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (非営利機関による非営利学術研究用)[Word]
            遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
            宮脇敦史博士による提供承諾書 [Word]
            備考 【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB05965 CS-CA-Venus DNA solution

            Ordering instruction of plasmids [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content


            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB16933_A9Blp1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CAG_R1
            Region: CAG pro,Venus 5'
            Sequence file: RDB16933_A9Bla.seq check
            >05965_16933_B2BfF_CAG_R1_C01_03_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNTNNNNNN GGCGCGGGGC GNAGCGGTGC GGCGCCGGCA
               61 GGAAGGAAAT GGGCGGGGAG GGCCTTCGTG CGTCGCCGCG CCGCCGTCCC CTTCTCCCTC
              121 TCCAGCCTCG GGGCTGTCCG CGGGGGGACG GCTGCCTTCG GGGGGGACGG GGCAGGGCGG
              181 GGTTCGGCTT CTGGCGTGTG ACCGGCGGCT CTAGAGCCTC TGCTAACCAT GTTCATGCCT
              241 TCTTCTTTTT CCTACAGCTC CTGGGCAACG TGCTGGTTAT TGTGCTGTCT CATCATTTTG
              301 GCAAAGAATT GATTTGATAC GTACGGTTGG GCTGCAGGAA TTGATCCACC ATGGTGAGCA
              361 AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA
              421 ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA
              481 CCCTGAAGCT GATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA
              541 CCCTGGGCTA CGGCCTGCAG TGCTTCGCCC GCTACCCCGA CCACATGAAG CAGCACGACT
              601 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG
              661 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA
              721 TCGAGCTGAA GGGCATCGAC TTCNAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT
              781 ACAACTACAA CAGCCACAAC GTCTATATCA CCGCCGANNA GCAGAAGANC GGCATCAAGG
              841 CCNACTTCNA GATCCGCCAC AACATCGNNG ANGGCGGCGT GCAGCTCGCC NANNNCTACC
              901 NGCNNANCNC CNNCNTCGNC GANNGNCCCN NGCNGCTGNC CGANAACCAC TANCTGANCT
              961 ANCNNTCCNN CCTNANCNAA GANCCNNACG AGAANCNNGN TNNNNTNGNN NNNGNTNGAN
            1021 TTCNNNNNNN NNN
            //
            Primer: PRE_rev
            Region: PRE,Venus 3'
            Sequence file: RDB16933_A9Blb.seq check
            >05965_16933_B2BfF_PRE_rev_D01_04_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNG NANNNNGAGC AACATAGTTN NNANTACCNG TCAATCTTTC
               61 ACAAATTTTG TAATCCAGAG GTTGATTATC GATAAGCTTG ATCCCTCGAG AGGCCTTGAA
              121 TTCTTACTTG TACAGCTCGT CCATGCCGAG AGTGATCCCG GCGGCGGTCA CGAACTCCAG
              181 CAGGACCATG TGATCGCGCT TCTCGTTGGG GTCTTTGCTC AGGGCGGACT GGTAGCTCAG
              241 GTAGTGGTTG TCGGGCAGCA GCACGGGGCC GTCGCCGATG GGGGTGTTCT GCTGGTAGTG
              301 GTCGGCGAGC TGCACGCCGC CGTCCTCGAT GTTGTGGCGG ATCTTGAAGT TGGCCTTGAT
              361 GCCGTTCTTC TGCTTGTCGG CGGTGATATA GACGTTGTGG CTGTTGTAGT TGTACTCCAG
              421 CTTGTGCCCC AGGATGTTGC CGTCCTCCTT GAAGTCGATG CCCTTCAGCT CGATGCGGTT
              481 CACCAGGGTG TCGCCCTCGA ACTTCACCTC GGCGCGGGTC TTGTAGTTGC CGTCGTCCTT
              541 GAAGAAGATG GTGCGCTCCT GGACGTAGCC TTCGGGCATG GCGGACTTGA AGAAGTCGTG
              601 CTGCTTCATG TGGTCGGGGT AGCGGGCGAA GCACTGCAGG CCGTAGCCCA GGGTGGTCAC
              661 GAGGGTGGGC CAGGGCACGG GCAGCTTGCC GGTGGTGCAG ATCAGCTTCA GGGTCAGCTT
              721 GCCGTANGTG GCATCGCCCT CGCCCTCGCC GGACACGCTG AACTTGTGGN CGTTTACGTC
              781 GCCGTCCAGC TCGACNAGGA TGGGCACCAC CCCGGTGAAC AGCTCCTCGC CCTTGCTCAC
              841 CATGGTGGNT CNATTCCTGC AGCCCNACCG TACGTATCNA ATCNATTCTT TGCCNAAATG
              901 ATGANACAGC ACAATNNCCN GCACGTNGNC NNNGANCTGT ANGAAAAANA ANAANGNATG
              961 AANNTNGTTN GCAGANGNTC TANANCCGNC NNNNNCNNNN NNNANNNCNG NNNCNCGCCN
            1021 NNNCCNNNNC CCNNCCNNNN NNNNNN
            //
            Primer: CMV_Forward
            Region: CMV pro,5'LTR,HIV-1 psi
            Sequence file: RDB16933_A9Blc.seq check
            >05965_16933_A9Bl_CMV_Forward_G01_07_ABI08.ab1
                1 NNNNNNNNNN NNNNCCCNNT TGNNGCNNNN GGGCGGNAGG CGTGTACGGT GGGAGGTCTA
               61 TATAAGCAGC GCGTTTTGCC TGTACTGGGT CTCTCTGGTT AGACCAGATC TGAGCCTGGG
              121 AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA ATAAAGCTTG CCTTGAGTGC
              181 TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA CTAGAGATCC CTCAGACCCT
              241 TTTAGTCAGT GTGGAAAATC TCTAGCAGTG GCGCCCGAAC AGGGACTTGA AAGCGAAAGG
              301 GAAACCAGAG GAGCTCTCTC GACGCAGGAC TCGGCTTGCT GAAGCGCGCA CGGCAAGAGG
              361 CGAGGGGCGG CGACTGGTGA GTACGCCAAA AATTTTGACT AGCGGAGGCT AGAAGGAGAG
              421 AGATGGGTGC GAGAGCGTCA GTATTAAGCG GGGGAGAATT AGATCGCGAT GGGAAAAAAT
              481 TCGGTTAAGG CCAGGGGGAA AGAAAAAATA TAAATTAAAA CATATAGTAT GGGCAAGCAG
              541 GGAGCTAGAA CGATTCGCAG TTAATCCTGG CCTGTTAGAA ACATCAGAAG GCTGTAGACA
              601 AATACTGGGA CAGCTACAAC CATCCCTTCA GACAGGATCA GAAGAACTTA GATCATTATA
              661 TAATACAGTA GCAACCCTCT ATTGTGTGCA TCAAAGGATA GAGATAAAAG ACACCAAGGA
              721 AGCTTTAGAC AAGATAGAGG AAGAGCAAAA CAAAAGTAAG ACCACCGCAC AGCAAGCGGC
              781 CGCTGATCTT CAGACCTGGA GGAGGAGATA TGANGGANAA TTGGAGAAGT GANTTATATA
              841 AATATAAAGT AGTAAAAATT GNNCCNTTAN GAGTANCACC CACCNAGGCA AAGAGAAGAG
              901 TGGTGCAGAG AGAAAAAAGA NCANNGGGAN TAGGAGCTTT GTTCCNTGGG TTCTTGGGAN
            961 CNNCNNNNGC ANTATGGGGC GCNNNNNCNA TGNNNCTGNN GGNANNNNNN NNNN
            //
            Primer: RRE_F1
            Region: RRE
            Sequence file: RDB16933_A9Bld.seq check
            >05965_16933_B2BfF_RRE_F1_G01_07_ABI08.ab1
                1 NNNNNNNNNN NNNNNCNNNN NNNNNNNNTG CTGANGGCTA TTGAGGCGCA ACAGCATCTG
               61 TTGCAACTCA CAGTCTGGGG CATCAAGCAG CTCCAGGCAA GAATCCTGGC TGTGGAAAGA
              121 TACCTAAAGG ATCAACAGCT CCTGGGGATT TGGGGTTGCT CTGGAAAACT CATTTGCACC
              181 ACTGCTGTGC CTTGGAATGC TAGTTGGAGT AATAAATCTC TGGAACAGAT TTGGAATCAC
              241 ACGACCTGGA TGGAGTGGGA CAGAGAAATT AACAATTACA CAAGCTTAAT ACACTCCTTA
              301 ATTGAAGAAT CGCAAAACCA GCAAGAAAAG AATGAACAAG AATTATTGGA ATTAGATAAA
              361 TGGGCAAGTT TGTGGAATTG GTTTAACATA ACAAATTGGC TGTGGTATAT AAAATTATTC
              421 ATAATGATAG TAGGAGGCTT GGTAGGTTTA AGAATAGTTT TTGCTGTACT TTCTATAGTG
              481 AATAGAGTTA GGCAGGGATA TTCACCATTA TCGTTTCAGA CCCACCTCCC AACCCCGAGG
              541 GGACCCGACA GGCCCGAAGG AATAGAAGAA GAAGGTGGAG AGAGAGACAG AGACAGATCC
              601 ATTCGATTAN TGAACGGNTC TACNAATGGC AGTATTCATC CNCNATTTTA AAAGAAAAGG
              661 GGGGNNTGGG GGGGNACAGN GCNNGGGNNN NNAGTAGACN TNANNNCNNN NGANNTNNNA
            721 NCTNANNNAT TNCNAANNNA NNNNNNNAAA ANNNNNAANN TTTNNN
            //
            Primer: env_F
            Region: cPPT_CTS,CAG pro
            Sequence file: RDB16933_A9Ble.seq check
            >05965_16993_A9Bl_1_env_F_D10_10_ABI24.ab1
                1 CTACNAATGG CAGTATTCAT CCNCAATTTT AAAAGAAAAG GGGGGATTGG GGGGTACAGT
               61 GCAGGGGAAA GAATAGTAGA CATAATAGCA ACAGACATAC AAACTAAAGA ATTACAAAAA
              121 CAAATTACAA AAATTCAAAA TTTTCGGGTT TATTACAGGG ACAGCAGAAA TTCACTTTGA
              181 ATTGGGCTGC AGGAATCTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC
              241 CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC
              301 AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG
              361 ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT
              421 CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC
              481 TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA
              541 TTAGTCATCG CTATTACCAT GGTCGAGGTG AGCCCCACGT TCTGCTTCAC TCTCCCCATC
              601 TCCCCCCCCT CCCCACCCCC AATTTTGTAT TTATTTATTT TTTAATTATT TTGTGCAGCG
            661 ATGGGGGCGG GGGGGGGGGG G
            //
            Primer: bGH_rev3
            Region: bGH pA,3'LTR
            Sequence file: RDB16933_A9Blf.seq check
            >05965_16933_B2BfF_bGH_rev3_D01_04_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG ATNGGCTGGC AACTAGAAGG
               61 CACAGTCGAG GCTGATCNNC GGGTTTAAAC GGGCCCTGCT AGAGATTTTC CACACTGACT
              121 AAAAGGGTCT GAGGGATCTC TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT
              181 GAAGCACTCA AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA
              241 GCTCCCAGGC TCAGATCTGG TCTAACCAGA GAGACCCAGT ACAGTCCGGA TGCAGCTCTC
              301 GGGCCATGTG ATGAAATGCT AGGCGGCTGT CAAACCTCCA CTCTAATACT TCTCTCTCCG
              361 GGTCATCCAT CCCATGCAGG CTCACAGGGT GTAACAAGCG GGTGTTCTCT CCTTCATTGG
              421 CTTCTTCTAC CTTCTCTTGC TCAACTGGTA CTAGCTTGTA GCACCATCCA AAGGTCAGTG
              481 GATATCTGAT CCCTGGCCCT GGTGTGTAGT TCTGCCAATC AGGGAAGTAG CCTTGTGTGT
              541 GGTAGATCCA CAGATCAAGG ATATCTTGTC TTCGTTGGGA GTGAATTAGC CCTTCCAGTC
              601 CCCCCTTTTC NTTTAAAAAG TGGCTAANAN CTACAGCTGC CTTGTAAGTC ATTGGTCTTA
              661 AANGNACCTG NNNGTGANTG GAAAANCCNN CTCCTCCTCC TCTNGNGCTT CNNNCCNGGC
            721 NCNATCAGCN NNNNNNNNN
            //
            Primer: SV40pro_F
            Region: SV40 pro_ori,Zeo,SV40 pA
            Sequence file: RDB16933_A9Blg.seq check
            >05965_16993_A9Bl_1_SV40pro_F_E10_13_ABI24.ab1
                1 GTGAGGANGN TTTTTTGGAG GNCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC
               61 CATTTTCGGA TCTGATCAGC ACGTGTTGAC AATTAATCAT CGGCATAGTA TATCGGCATA
              121 GTATAATACG ACAAGGTGAG GAACTAAACC ATGGCCAAGT TGACCAGTGC CGTTCCGGTG
              181 CTCACCGCGC GCGACGTCGC CGGAGCGGTC GAGTTCTGGA CCGACCGGCT CGGGTTCTCC
              241 CGGGACTTCG TGGAGGACGA CTTCGCCGGT GTGGTCCGGG ACGACGTGAC CCTGTTCATC
              301 AGCGCGGTCC AGGACCAGGT GGTGCCGGAC AACACCCTGG CCTGGGTGTG GGTGCGCGGC
              361 CTGGACGAGC TGTACGCCGA GTGGTCGGAG GTCGTGTCCA CGAACTTCCG GGACGCCTCC
              421 GGGCCGGCCA TGACCGAGAT CGGCGAGCAG CCGTGGGGGC GGGAGTTCGC CCTGCGCGAC
              481 CCGGCCGGCA ACTGCGTGCA CTTCGTGGCC GAGGAGCAGG ACTGACACGT GCTACGAGAT
              541 TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC
              601 GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG
              661 TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA
              721 GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT
              781 GTCTGTATAC CGTCGANCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT
              841 GTGTGAAATT GTTATCCGCT CACNATTCCA CACNACATAC GAGCCGGAAG CATNAAGTGT
              901 AAAGNCNGGG GNGNCNNATG ANTGANCTAN NTCANNTTAA TTGCNTTGCN CTCACTGCCC
            961 NCTTTNCAG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            user_report Nakajima, R., A noncoding RNA containing a SINE-B1 motif associates with meiotic metaphase chromatin and has an indispensable function during spermatogenesis. PLoS One 12 (6): e0179585 (2017). PMID 28658256.

            2022.08.12

            GNP_filter3_RDBDEP_html_220812.pl