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CS-CA-GFP (#RDB05964)

Lentivirus vector plasmid of GFP driven by CAG promoter.

Clone info. Lentivirus vector plasmid of GFP driven by CAG promoter.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone Plasmid
Selectable markers Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth conditions 37C, LB+Amp
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Miyoshi, Hiroyuki |
 
Sequence (full) RDB05964hts01.seq checkAssembled from experimentally sequenced data.
Remarks, protocol and/or map (pdf) RDB05964.pdf
Publication Unpublished bioresource
Test sheet RDB15875_A8D9p1-2.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose)[Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利機関による非営利学術研究用)[Word] [MTAの記入例 ]
遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB05964 CS-CA-GFP DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

How to cite this biological resource

Materials & Methods section:

The CS-CA-GFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05964).

Reference section:

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

user_report Jargalsaikhan, B.E., The Dual-Pseudotyped Lentiviral Vector with VSV-G and Sendai Virus HN Enhances Infection Efficiency through the Synergistic Effect of the Envelope Proteins. Viruses 16 (6): 827 (2024). PMID 38932120. [link to RRC of NBRP]
user_report Komatsu, M., Cell-cycle dependent GATA2 subcellular localization in mouse 2-cell embryos. Biochem. Biophys. Res. Commun. 584: 1-6 (2021). PMID 34741809. [link to RRC of NBRP]
user_report Kishimoto, W., Expression of Tim-1 in primary CNS lymphoma. Cancer Med. 5 (11): 3235-3245 (2017). PMID 27709813. [link to RRC of NBRP]
user_report Fujita, K., Effects of a brain-engraftable microglial cell line expressing anti-prion scFv antibodies on survival times of mice infected with scrapie prions. Cell. Mol. Neurobiol. 31 (7): 999-1008 (2011). PMID 21516351. [link to RRC of NBRP]