Resource data sheet
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CS-CA-GFP

Lentivirus vector construct of GFP driven by CAG promoter.

Catalog number RDB05964
Resource name CS-CA-GFP
Clone info. Lentivirus vector construct of GFP driven by CAG promoter.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone Plasmid
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name Aequorea victoria GFP cDNA
Originator Miyoshi, Hiroyuki |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi as the Developer.
Ordering Please visit Ordering instruction. 
Order form 
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Material Transfer Agreement (MTA) [Word]
Remarks Remember that you will be working with samples containing infectious virus.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB05964 CS-CA-GFP DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB05964z.seq from Depositor
Electronic file Remarks, protocol and/or map (pdf) RDB05964.pdf from Depositor

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15875_A8Cfp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: CAGpro,EGFP5' | Primer: pCAG-F_V2 | Sequence: RDB15875_A8Cfe.seq
>05964_15875_A9I5F_pCAG-F_V2_A08_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNTGNGC GGNNCCGANT CTGGGNNGNG CCGCCGCACC CCCTCTAGCG
   61 GGCGCGGGGC GAAGCGGTGC GGCGCCGGCA GGAAGGAAAT GGGCGGGGAG GGCCTTCGTG
  121 CGTCGCCGCG CCGCCGTCCC CTTCTCCCTC TCCAGCCTCG GGGCTGTCCG CGGGGGGACG
  181 GCTGCCTTCG GGGGGGACGG GGCAGGGCGG GGTTCGGCTT CTGGCGTGTG ACCGGCGGCT
  241 CTAGAGCCTC TGCTAACCAT GTTCATGCCT TCTTCTTTTT CCTACAGCTC CTGGGCAACG
  301 TGCTGGTTAT TGTGCTGTCT CATCATTTTG GCAAAGAATT GATTTGATAC GTACGGTTGG
  361 GCTGCAGGAA TTCGATGCTA CCGGTCGCCA CCATGGTGAG CAAGGGCGAG GAGCTGTTCA
  421 CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG
  481 TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA
  541 CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGACC TACGGCGTGC
  601 AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC
  661 CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC
  721 GCGCCGANGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG
  781 ACTTCAAGGN NGANGGCAAC ATCCTGGGGC ACAAGCTGGA NTACNACTAC NACAGCCACA
  841 ACGTCTATAT CATGGCCGAN AAGCAGAAGA NCGGNNTCNA GGNGAACTTC NAGATCCNNC
901 ACAACATCNN NGNNGGNAGC
//
Region: HIV-1psi,5'LTR,CMV pro,CMVen | Primer: HIV-B_R | Sequence: RDB15875_A8Cff.seq
>05964_15875_A9I5F_HIV-B_R_B08_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTT TGGNGTANTC NCCNGTCGCC GCCCCTCGCC
   61 TCTTGCCGTG CGCGCTTCAG CAAGCCGAGT CCTGCGTCGA GAGAGCTCCT CTGGTTTCCC
  121 TTTCGCTTTC AAGTCCCTGT TCGGGCGCCA CTGCTAGAGA TTTTCCACAC TGACTAAAAG
  181 GGTCTGAGGG ATCTCTAGTT ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC
  241 ACTCAAGGCA AGCTTTATTG AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC
  301 CAGGCTCAGA TCTGGTCTAA CCAGAGAGAC CCAGTACAGG CAAAACGCGC TGCTTATATA
  361 GACCTCCCAC CGTACACGCC TACCGCCCAT TTGCGTCAAT GGGGCGGAGT TGTTACGACA
  421 TTTTGGAAAG TCCCGTTGAT TTTGGTGCCA AAACAAACTC CCATTGACGT CAATGGGGTG
  481 GAGACTTGGA AATCCCCGTG AGTCAAACCG CTATCCACGC CCATTGATGT ACTGCCAAAA
  541 CCGCATCACC ATGGTAATAG CGATGACTAA TACGTAGATG TACTGCCAAG TAGGAAAGTC
  601 CCATAAGGTC ATGTACTGGG CATAATGCCA GGCGGGCCAT TTACCGTCAT TGACGTCAAT
  661 AGGGGGCGTA CTTGGCATAT GATACACTTG ATGTACTGCC AAGTGGGCAG TTTACCGTAA
  721 ATACTCCACC CATTGACGTC AATGGAAAGT CCCTATTGGC GTTACTATGG GAACATACGT
  781 CATTATTGAC GTCAATGGGC GGGGGTCGTT GGGCGGTCAG CCAGGCGGGC CATTTACCGT
  841 AAGTTATGTA ACGCGGANNT CCATATATGG GCTATGAACT AATGANNCCG TAATTGATTA
  901 CTATTAATAA CTAGNCAATA ATCAATGTCA ACGCGTATAT CTGGNCNNNA NATCGCNNAN
961 CAGCGCN
//
Region: RRE,HIV-1psi,5'LTR | Primer: RRE-M_R | Sequence: RDB15875_A8Cfg.seq
>05964_15875_A9I5F_RRE-M_R_D08_11_ABI24.ab1
    1 NNNNNNNNNN NNANNNNNGC TGTTGCGCCT CNATAGCCCT CAGCAAATTG TTCTGCTGCT
   61 GCACTATACC AGACAATAAT TGTCTGGCCT GTACCGTCAG CGTCATTGAC GCTGCGCCCA
  121 TAGTGCTTCC TGCTGCTCCC AAGAACCCAA GGAACAAAGC TCCTATTCCC ACTGCTCTTT
  181 TTTCTCTCTG CACCACTCTT CTCTTTGCCT TGGTGGGTGC TACTCCTAAT GGTTCAATTT
  241 TTACTACTTT ATATTTATAT AATTCACTTC TCCAATTGTC CCTCATATCT CCTCCTCCAG
  301 GTCTGAAGAT CAGCGGCCGC TTGCTGTGCG GTGGTCTTAC TTTTGTTTTG CTCTTCCTCT
  361 ATCTTGTCTA AAGCTTCCTT GGTGTCTTTT ATCTCTATCC TTTGATGCAC ACAATAGAGG
  421 GTTGCTACTG TATTATATAA TGATCTAAGT TCTTCTGATC CTGTCTGAAG GGATGGTTGT
  481 AGCTGTCCCA GTATTTGTCT ACAGCCTTCT GATGTTTCTA ACAGGCCAGG ATTAACTGCG
  541 AATCGTTCTA GCTCCCTGCT TGCCCATACT ATATGTTTTA ATTTATATTT TTTCTTTCCC
  601 CCTGGCCTTA ACCGAATTTT TTCCCATCGC GATCTAATTC TCCCCCGCTT AATACTGACG
  661 CTCTCGCACC CATCTCTCTC CTTCTAGCCT CCGCTAGTCA AAATTTTTGG CGTACTCACC
  721 AGTCGCCGCC CCTCGCCTCT TGCCGTGCGC GCTTCAGCAA GCCGAGTCCT GCGTCGAGAG
  781 AGCTCCTCTG GTTTCCCTTT CGCTTTCAAG TCCCTGTTCG GGCGCCACTG CTAGAGATTT
841 TCCACACTGA CTAAAANGGT CTGANGGN
//
Region: cPPT_CTS.CAGpro | Primer: env_F | Sequence: RDB15875_A8Cfh.seq
>05964_15875_A8Cf_env_F_G01_07_ABI08.ab1
    1 AAGGGAGCAG GAATCATTCG ATTAGTGAAC GGATCTACAA ATGGGCAGGT ATTACATCCA
   61 CAATTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT
  121 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAA TTCAAAATTT
  181 TCGGGTTTAT TACAGGGACA GCAGAAATTC ACTTTGAATT GGGCTGCAGG AATCTAGTTA
  241 TTAATAGTAA TCAATTACGG GGTCATTAGT TCATAGCCCA TATATGGAGT TCCGCGTTAC
  301 ATAACTTACG GTAAATGGCC CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC
  361 AATAATGACG TATGTTCCCA TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT
  421 GGAGTATTTA CGGTAAACTG CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC
  481 GCCCCCTATT GACGTCAATG ACGGTAAATG GCCCGCCTGG CATTATGCCC AGTACATGAC
  541 CTTATGGGAC TTTCCTACTT GGCAGTACAT CTACGTATTA GTCATCGCTA TTACCATGGT
  601 CGAGGTGAGC CCCACGTTCT GCTTCACTCT CCCCATCTCC CCCCCCTCCC CACCCCCAAT
  661 TTTGTATTTA TTTATTTTTT AATTATTTTG TGCAGCGATG GGGGCGGGGG GGGGGGGGGG
//
Region: EFGP,WPRE | Primer: pAxCA_F1 | Sequence: RDB15875_A8Cfi.seq
>05964_15875_A8Cf_pAxCA_F1_F02_17_ABI24.ab1
    1 CCATCTTTTC TACATGTTCA TGCCTTCTTC TTTTTCCTAC AGCTCCTGGG CAACGTGCTG
   61 GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTGATTT GATACGTACG GTTGGGCTGC
  121 AGGAATTCGA TGCTACCGGT CGCCACCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG
  181 GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC
  241 GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC
  301 GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC
  361 TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA
  421 GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC
  481 GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC
  541 AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC
  601 TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC
  661 ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC
  721 GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC
  781 CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT
  841 CTCGGCATGG ACGAGCTGTA CAAGTCCGGA CTCAGATCCT ACTAGTAGGA TCTCGAGGGA
  901 TCAAGCTTAT CGATAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC
  961 TTAACTATGT TGCTCCCTTT TTACGCTATG TGGATACGCT GCTTTAATGC CCTTTGTATC
 1021 ATGCTATTGC TTTCCCGTAT GGCTTTCATT TTTCTCCTTC CTGTATAAAT TCCTTGGTTG
1081 CCTGATCTCA TTTAATGGAG AAGTTGGTGG CCCGGTTTGT TCCGA
//
Region: 3’LTR,WPRE | Primer: BGH_rev | Sequence: RDB15875_A8Cfj.seq
>05964_15875_A8Cf_BGH_rev_H02_23_ABI24.ab1
    1 GTTGGTACGG GGGTCTGCGT GAGGTTTTGC ACACTGACTA AAAGGGTCGT GAGGGATCTG
   61 CTAGTGTACC AGAGTCGACA CAACAGACGG GCACAGCACT ACTTGAAGCA CTCAAGGCAA
  121 GCTTTATTGA GGGTTAAGCA GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC AGGCTCAGAT
  181 CTGGTCTAAC CAGAGAGACC CAGTACAGTC CGGATGCAGC TCTCGGGCCA TGTGATGAAA
  241 TGCTAGGCGG CTGTCAAACC TCCACTCTAA TACTTCTCTC TCCGGGTCAT CCATCCCATG
  301 CAGGCTCACA GGGTGTAACA AGCGGGTGTT CTCTCCTTCA TTGGCTTCTT CTACCTTCTC
  361 TTGCTCAACT GGTACTAGCT TGTAGCACCA TCCAAAGGTC AGTGGATATC TGATCCCTGG
  421 CCCTGGTGTG TAGTTCTGCC AATCAGGGAA GTAGCCTTGT GTGTGGTAGA TCCACAGATC
  481 AAGGATATCT TGTCTTCGTT GGGAGTGAAT TAGCCCTTCC AGTCCCCCCT TTTCTTTTAA
  541 AAAGTGGCTA AGATCTACAG CTGCCTTGTA AGTCATTGGT CTTAAAGGTA CCTGAGGTGT
  601 GACTGGAAAA CCCACCTCCT CCTCCTCTTG TGCTTCTAGC CAGGCACAAT CAGCATTGGT
  661 AGCTGCTGTA TTGCTACTTG TGATTGCTCC ATGTTTTTCT AGGTCTCGAC GGTATCGATG
  721 CGGGGAGGCG GCCCAAAGGG AGATCCGACT CGTCTGAGGG CGAAGGCGAA GACGCGGAAG
  781 AGGCCGCAGA GCCGGCAGCA GGCCGCGGGA AGGAAGGTCC GCTGGATTGA GGGCCGAAGG
  841 GACGTAGCAG AAGGACGTCC CGCGCAGAAT CCAGGTGGCA ACACAGGCGA GCAGCCATGG
  901 AAAGGACGTC AGCTTCCCCG ACAACACCAC GGAATTGTCA GTGCCCAACA GCCGAGCCCC
  961 TGTCCAGCAG CGGGCAAAGG CAGGCGGCGA TGAGTTCCCG CCGTGGCAAT AGGGAGGGGG
 1021 AAAGCGAAAG TCCCGGAAAG GAGCTGAACA GGTGAGGCAA TGCCCCCACC AGTTGGGGAT
 1081 GCGTCAGCCA ACACAGGTGC AACTACGTCA ACGGTTGTCT GGACAACCGG GTCCTACACA
1141 ACTTCCCTTC AT
//
Region: SV40pro_ori,EM7pro,Zeo,SV40p(A) | Primer: SV40pro_F | Sequence: RDB15875_A8Cfk.seq
>05964_15875_A8Cf_SV40pro_F_A03_03_ABI24.ab1
    1 GCCTGGGTCC CTTAACATTC AGAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC
   61 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAGCACGT GTTGACAATT
  121 AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA GGTGAGGAAC TAAACCATGG
  181 CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA CGTCGCCGGA GCGGTCGAGT
  241 TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA GGACGACTTC GCCGGTGTGG
  301 TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA CCAGGTGGTG CCGGACAACA
  361 CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA CGCCGAGTGG TCGGAGGTCG
  421 TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC CGAGATCGGC GAGCAGCCGT
  481 GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG CGTGCACTTC GTGGCCGAGG
  541 AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG
  601 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC
  661 TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA
  721 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT
  781 CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG
  841 CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA
  901 ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA
  961 CATTAATTGC GTTGCGCTCA CTGCCCGCTT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG
 1021 CATTAAATGA ATCGGCCAAC GGCGCGGGGG AGAGCGATTT GCGTATTGGG CGCTCTTCGC
1081 TCCTTCGGCT CACTGACTTC GGCTGGCGGC CTCCGGATCC GT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

user report Kishimoto, W., Expression of Tim-1 in primary CNS lymphoma. Cancer Med. 5 (11): 3235-3245 (2017). PMID 27709813.
user report Fujita, K., Effects of a brain-engraftable microglial cell line expressing anti-prion scFv antibodies on survival times of mice infected with scrapie prions. Cell. Mol. Neurobiol. 31 (7): 999-1008 (2011). PMID 21516351.

2019.10.01

GNP_filter3_RDBDEP_html_190927.pl