Resource data sheet
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CS-CA-GFP

Lentivirus vector construct of GFP driven by CAG promoter.

Catalog number RDB05964
Resource name CS-CA-GFP
Clone info. Lentivirus vector construct of GFP driven by CAG promoter.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone Plasmid
Selectable markers Amp^r (E. coli); Zeo^r (plasmid transfection of mammalian cells).
Gene/insert name Aequorea victoria GFP cDNA
Originator Miyoshi, Hiroyuki |

Distribution information

CLICK here for plasmid list and map. [link]
Forms for plasmid request [in Japanese] [in English]
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
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Remarks Remember that you will be working with samples containing infectious virus.
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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15875_A8Cfp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 RDB15875_A8Cfa.seq
>08230_15875_A8Cf_pAxCA_F1_F02_17_ABI24.ab1
    1 CCATCTTTTC TACATGTTCA TGCCTTCTTC TTTTTCCTAC AGCTCCTGGG CAACGTGCTG
   61 GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTGATTT GATACGTACG GTTGGGCTGC
  121 AGGAATTCGA TGCTACCGGT CGCCACCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG
  181 GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC
  241 GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC
  301 GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC
  361 TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA
  421 GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC
  481 GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC
  541 AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC
  601 TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC
  661 ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC
  721 GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC
  781 CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT
  841 CTCGGCATGG ACGAGCTGTA CAAGTCCGGA CTCAGATCCT ACTAGTAGGA TCTCGAGGGA
  901 TCAAGCTTAT CGATAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC
  961 TTAACTATGT TGCTCCCTTT TTACGCTATG TGGATACGCT GCTTTAATGC CCTTTGTATC
 1021 ATGCTATTGC TTTCCCGTAT GGCTTTCATT TTTCTCCTTC CTGTATAAAT TCCTTGGTTG
1081 CCTGATCTCA TTTAATGGAG AAGTTGGTGG CCCGGTTTGT TCCGA
//
Primer: HIVgp_R1 RDB15875_A8Cfb.seq
>08230_15875_A8Cf_HIVgp_R1_G02_20_ABI24.ab1
    1 CAGCATTCTA TTCCACTGCT CTTTTTTCTC TCTGCACCAC TCTTCTCTTT GCCTTGGTGG
   61 GTGCTACTCC TAATGGTTCA ATTTTTACTA CTTTATATTT ATATAATTCA CTTCTCCAAT
  121 TGTCCCTCAT ATCTCCTCCT CCAGGTCTGA AGATCAGCGG CCGCTTGCTG TGCGGTGGTC
  181 TTACTTTTGT TTTGCTCTTC CTCTATCTTG TCTAAAGCTT CCTTGGTGTC TTTTATCTCT
  241 ATCCTTTGAT GCACACAATA GAGGGTTGCT ACTGTATTAT ATAATGATCT AAGTTCTTCT
  301 GATCCTGTCT GAAGGGATGG TTGTAGCTGT CCCAGTATTT GTCTACAGCC TTCTGATGTT
  361 TCTAACAGGC CAGGATTAAC TGCGAATCGT TCTAGCTCCC TGCTTGCCCA TACTATATGT
  421 TTTAATTTAT ATTTTTTCTT TCCCCCTGGC CTTAACCGAA TTTTTTCCCA TCGCGATCTA
  481 ATTCTCCCCC GCTTAATACT GACGCTCTCG CACCCATCTC TCTCCTTCTA GCCTCCGCTA
  541 GTCAAAATTT TTGGCGTACT CACCAGTCGC CGCCCCTCGC CTCTTGCCGT GCGCGCTTCA
  601 GCAAGCCGAG TCCTGCGTCG AGAGAGCTCC TCTGGTTTCC CTTTCGCTTT CAAGTCCCTG
  661 TTCGGGCGCC ACTGCTAGAG ATTTTCCACA CTGACTAAAA GGGTCTGAGG GATCTCTAGT
  721 TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG CACTCAAGGC AAGCTTTATT
  781 GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC CCAGGCTCAG ATCTGGTCTA
  841 ACCAGAGAGA CCCAGTACAG GCAAAACGCG CTGCTTATAT AGACCTCCCA CCGTACACGC
  901 CTACCGCCCA TTTGCGTCAA TGGGGGCGGA GTTGTTACGA CATTTTGGAA AGTCCCGTTG
  961 ATTTTGGGTG CCCAAAACAA ACTCCCATTG ACGTCAATGG GGGTGGAGAC TTTGGAAATC
 1021 CCCGTGAGTC AAACCGCTAA TCCACGCCCA TTGATGTACT GCAAAAACGC CATCACCATG
 1081 GGTTATAGCG ATGGACCTAA ATACGGTAGA TGTAACCTGC TAAGCTAGAA GTCTATAAGG
1141 TCCAATGGT
//
Primer: BGH_rev RDB15875_A8Cfc.seq
>08230_15875_A8Cf_BGH_rev_H01_08_ABI08.ab1
    1 GTTAAAGCGG GGGCCCTGCT AGAGATTTTC CACACTGACT AAAAGGGTCT GAGGGATCTC
   61 TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT GAAGCACTCA AGGCAAGCTT
  121 TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA GCTCCCAGGC TCAGATCTGG
  181 TCTAACCAGA GAGACCCAGT ACAGTCCGGA TGCAGCTCTC GGGCCATGTG ATGAAATGCT
  241 AGGCGGCTGT CAAACCTCCA CTCTAATACT TCTCTCTCCG GGTCATCCAT CCCATGCAGG
  301 CTCACAGGGT GTAACAAGCG GGTGTTCTCT CCTTCATTGG CTTCTTCTAC CTTCTCTTGC
  361 TCAACTGGTA CTAGCTTGTA GCACCATCCA AAGGTCAGTG GATATCTGAT CCCTGGCCCT
  421 GGTGTGTAGT TCTGCCAATC AGGGAAGTAG CCTTGTGTGT GGTAGATCCA CAGATCAAGG
  481 ATATCTTGTC TTCGTTGGGA GTGAATTAGC CCTTCCAGTC CCCCCTTTTC TTTTAAAAAG
  541 TGGCTAAGAT CTACAGCTGC CTTGTAAGTC ATTGGTCTTA AAGGTACCTG AGGTGTGACT
  601 GGAAAACCCA CCTCCTCCTC CTCTTGTGCT TCTAGCCAGG CACAATCAGC ATTGGTAGCT
  661 GCTGTATTGC TACTTGTGAT TGCTCCATGT TTTTCTAGGT CTCGACGGTA TCGATGCGGG
  721 GAGGCGGCCC AAAGGGAGAT CCGACTCGTC TGAGGGCGAA GGCGAAGACG CGGAAGAGGC
  781 CGCAGAGCCG GCAGCAGGCC GCGGGAAGGA AGGTCCGCTG GATTGAGGGC CGAAGGGACG
  841 TAGCAGAAGG ACGTCCCGCG CAGAATCCAG GTGGCAACAC AGGCGAGCAG CCATGGAAAG
  901 GACGTCAGCT TCCCCGACAA CACCACGGAA TTTGTCAGTG CCCAACAGCC GAGCCCCTGT
  961 CCAGCAGCGG GCAAGGCAGG CGGCGATGAG TTCCCGCCGT GGCAATAGGG AGGGGGAAGC
 1021 GAAAGTCCCG GAAAGGGAGC TGACAGTGGT GCAATGCCCC AACCAGTGGG GGTTGCGTCA
 1081 GCAAACACAG TGCCAACCAC GGCAACGGTG CTTGAACAAC GGCTCAACAA CTTCCCTTCA
1141 TTAA
//
Primer: SV40pro_F RDB15875_A8Cfd.seq
>08230_15875_A8Cf_SV40pro_F_A03_03_ABI24.ab1
    1 GCCTGGGTCC CTTAACATTC AGAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC
   61 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAGCACGT GTTGACAATT
  121 AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA GGTGAGGAAC TAAACCATGG
  181 CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA CGTCGCCGGA GCGGTCGAGT
  241 TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA GGACGACTTC GCCGGTGTGG
  301 TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA CCAGGTGGTG CCGGACAACA
  361 CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA CGCCGAGTGG TCGGAGGTCG
  421 TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC CGAGATCGGC GAGCAGCCGT
  481 GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG CGTGCACTTC GTGGCCGAGG
  541 AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG
  601 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC
  661 TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA
  721 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT
  781 CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG
  841 CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA
  901 ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA
  961 CATTAATTGC GTTGCGCTCA CTGCCCGCTT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG
 1021 CATTAAATGA ATCGGCCAAC GGCGCGGGGG AGAGCGATTT GCGTATTGGG CGCTCTTCGC
1081 TCCTTCGGCT CACTGACTTC GGCTGGCGGC CTCCGGATCC GT
//

Please visit Sequencing and PCR primers for primer information.

References and tips

Electronic file

References

user report Kishimoto, W., Expression of Tim-1 in primary CNS lymphoma. Cancer Med. 5 (11): 3235-3245 (2017). PMID 27709813.
user report Fujita, K., Effects of a brain-engraftable microglial cell line expressing anti-prion scFv antibodies on survival times of mice infected with scrapie prions. Cell. Mol. Neurobiol. 31 (7): 999-1008 (2011). PMID 21516351.

2018.05.11

GNP_filter3_RDBDEP_html_171214.pl