Please review the QC test results indicated by icon below as well as clone information before placing your order.

CS-CA-GFP

Lentivirus vector plasmid of GFP driven by CAG promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering	Please visit Ordering instruction.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word] This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
Remarks	Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。 提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします （Schedule AとSchedule Bを入手[link])。 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15875_A8Cfp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F_V2 Region: CAGpro,EGFP5' Sequence file: RDB15875_A8Cfe.seq
>05964_15875_A9I5F_pCAG-F_V2_A08_02_ABI24.ab1     1 NNNNNNNNNN NNNNNTGNGC GGNNCCGANT CTGGGNNGNG CCGCCGCACC CCCTCTAGCG    61 GGCGCGGGGC GAAGCGGTGC GGCGCCGGCA GGAAGGAAAT GGGCGGGGAG GGCCTTCGTG   121 CGTCGCCGCG CCGCCGTCCC CTTCTCCCTC TCCAGCCTCG GGGCTGTCCG CGGGGGGACG   181 GCTGCCTTCG GGGGGGACGG GGCAGGGCGG GGTTCGGCTT CTGGCGTGTG ACCGGCGGCT   241 CTAGAGCCTC TGCTAACCAT GTTCATGCCT TCTTCTTTTT CCTACAGCTC CTGGGCAACG   301 TGCTGGTTAT TGTGCTGTCT CATCATTTTG GCAAAGAATT GATTTGATAC GTACGGTTGG   361 GCTGCAGGAA TTCGATGCTA CCGGTCGCCA CCATGGTGAG CAAGGGCGAG GAGCTGTTCA   421 CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG   481 TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA   541 CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGACC TACGGCGTGC   601 AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC   661 CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC   721 GCGCCGANGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG   781 ACTTCAAGGN NGANGGCAAC ATCCTGGGGC ACAAGCTGGA NTACNACTAC NACAGCCACA   841 ACGTCTATAT CATGGCCGAN AAGCAGAAGA NCGGNNTCNA GGNGAACTTC NAGATCCNNC 901 ACAACATCNN NGNNGGNAGC //
Primer: HIV-B_R Region: HIV-1psi,5'LTR,CMV pro,CMVen Sequence file: RDB15875_A8Cff.seq
>05964_15875_A9I5F_HIV-B_R_B08_05_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTT TGGNGTANTC NCCNGTCGCC GCCCCTCGCC    61 TCTTGCCGTG CGCGCTTCAG CAAGCCGAGT CCTGCGTCGA GAGAGCTCCT CTGGTTTCCC   121 TTTCGCTTTC AAGTCCCTGT TCGGGCGCCA CTGCTAGAGA TTTTCCACAC TGACTAAAAG   181 GGTCTGAGGG ATCTCTAGTT ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC   241 ACTCAAGGCA AGCTTTATTG AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC   301 CAGGCTCAGA TCTGGTCTAA CCAGAGAGAC CCAGTACAGG CAAAACGCGC TGCTTATATA   361 GACCTCCCAC CGTACACGCC TACCGCCCAT TTGCGTCAAT GGGGCGGAGT TGTTACGACA   421 TTTTGGAAAG TCCCGTTGAT TTTGGTGCCA AAACAAACTC CCATTGACGT CAATGGGGTG   481 GAGACTTGGA AATCCCCGTG AGTCAAACCG CTATCCACGC CCATTGATGT ACTGCCAAAA   541 CCGCATCACC ATGGTAATAG CGATGACTAA TACGTAGATG TACTGCCAAG TAGGAAAGTC   601 CCATAAGGTC ATGTACTGGG CATAATGCCA GGCGGGCCAT TTACCGTCAT TGACGTCAAT   661 AGGGGGCGTA CTTGGCATAT GATACACTTG ATGTACTGCC AAGTGGGCAG TTTACCGTAA   721 ATACTCCACC CATTGACGTC AATGGAAAGT CCCTATTGGC GTTACTATGG GAACATACGT   781 CATTATTGAC GTCAATGGGC GGGGGTCGTT GGGCGGTCAG CCAGGCGGGC CATTTACCGT   841 AAGTTATGTA ACGCGGANNT CCATATATGG GCTATGAACT AATGANNCCG TAATTGATTA   901 CTATTAATAA CTAGNCAATA ATCAATGTCA ACGCGTATAT CTGGNCNNNA NATCGCNNAN 961 CAGCGCN //
Primer: RRE-M_R Region: RRE,HIV-1psi,5'LTR Sequence file: RDB15875_A8Cfg.seq
>05964_15875_A9I5F_RRE-M_R_D08_11_ABI24.ab1     1 NNNNNNNNNN NNANNNNNGC TGTTGCGCCT CNATAGCCCT CAGCAAATTG TTCTGCTGCT    61 GCACTATACC AGACAATAAT TGTCTGGCCT GTACCGTCAG CGTCATTGAC GCTGCGCCCA   121 TAGTGCTTCC TGCTGCTCCC AAGAACCCAA GGAACAAAGC TCCTATTCCC ACTGCTCTTT   181 TTTCTCTCTG CACCACTCTT CTCTTTGCCT TGGTGGGTGC TACTCCTAAT GGTTCAATTT   241 TTACTACTTT ATATTTATAT AATTCACTTC TCCAATTGTC CCTCATATCT CCTCCTCCAG   301 GTCTGAAGAT CAGCGGCCGC TTGCTGTGCG GTGGTCTTAC TTTTGTTTTG CTCTTCCTCT   361 ATCTTGTCTA AAGCTTCCTT GGTGTCTTTT ATCTCTATCC TTTGATGCAC ACAATAGAGG   421 GTTGCTACTG TATTATATAA TGATCTAAGT TCTTCTGATC CTGTCTGAAG GGATGGTTGT   481 AGCTGTCCCA GTATTTGTCT ACAGCCTTCT GATGTTTCTA ACAGGCCAGG ATTAACTGCG   541 AATCGTTCTA GCTCCCTGCT TGCCCATACT ATATGTTTTA ATTTATATTT TTTCTTTCCC   601 CCTGGCCTTA ACCGAATTTT TTCCCATCGC GATCTAATTC TCCCCCGCTT AATACTGACG   661 CTCTCGCACC CATCTCTCTC CTTCTAGCCT CCGCTAGTCA AAATTTTTGG CGTACTCACC   721 AGTCGCCGCC CCTCGCCTCT TGCCGTGCGC GCTTCAGCAA GCCGAGTCCT GCGTCGAGAG   781 AGCTCCTCTG GTTTCCCTTT CGCTTTCAAG TCCCTGTTCG GGCGCCACTG CTAGAGATTT 841 TCCACACTGA CTAAAANGGT CTGANGGN //
Primer: env_F Region: cPPT_CTS.CAGpro Sequence file: RDB15875_A8Cfh.seq
>05964_15875_A8Cf_env_F_G01_07_ABI08.ab1     1 AAGGGAGCAG GAATCATTCG ATTAGTGAAC GGATCTACAA ATGGGCAGGT ATTACATCCA    61 CAATTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT   121 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAA TTCAAAATTT   181 TCGGGTTTAT TACAGGGACA GCAGAAATTC ACTTTGAATT GGGCTGCAGG AATCTAGTTA   241 TTAATAGTAA TCAATTACGG GGTCATTAGT TCATAGCCCA TATATGGAGT TCCGCGTTAC   301 ATAACTTACG GTAAATGGCC CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC   361 AATAATGACG TATGTTCCCA TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT   421 GGAGTATTTA CGGTAAACTG CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC   481 GCCCCCTATT GACGTCAATG ACGGTAAATG GCCCGCCTGG CATTATGCCC AGTACATGAC   541 CTTATGGGAC TTTCCTACTT GGCAGTACAT CTACGTATTA GTCATCGCTA TTACCATGGT   601 CGAGGTGAGC CCCACGTTCT GCTTCACTCT CCCCATCTCC CCCCCCTCCC CACCCCCAAT   661 TTTGTATTTA TTTATTTTTT AATTATTTTG TGCAGCGATG GGGGCGGGGG GGGGGGGGGG //
Primer: pAxCA_F1 Region: EFGP,WPRE Sequence file: RDB15875_A8Cfi.seq
>05964_15875_A8Cf_pAxCA_F1_F02_17_ABI24.ab1     1 CCATCTTTTC TACATGTTCA TGCCTTCTTC TTTTTCCTAC AGCTCCTGGG CAACGTGCTG    61 GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTGATTT GATACGTACG GTTGGGCTGC   121 AGGAATTCGA TGCTACCGGT CGCCACCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG   181 GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC   241 GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC   301 GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC   361 TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA   421 GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC   481 GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC   541 AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC   601 TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC   661 ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC   721 GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC   781 CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT   841 CTCGGCATGG ACGAGCTGTA CAAGTCCGGA CTCAGATCCT ACTAGTAGGA TCTCGAGGGA   901 TCAAGCTTAT CGATAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC   961 TTAACTATGT TGCTCCCTTT TTACGCTATG TGGATACGCT GCTTTAATGC CCTTTGTATC  1021 ATGCTATTGC TTTCCCGTAT GGCTTTCATT TTTCTCCTTC CTGTATAAAT TCCTTGGTTG 1081 CCTGATCTCA TTTAATGGAG AAGTTGGTGG CCCGGTTTGT TCCGA //
Primer: BGH_rev Region: 3’LTR,WPRE Sequence file: RDB15875_A8Cfj.seq
>05964_15875_A8Cf_BGH_rev_H02_23_ABI24.ab1     1 GTTGGTACGG GGGTCTGCGT GAGGTTTTGC ACACTGACTA AAAGGGTCGT GAGGGATCTG    61 CTAGTGTACC AGAGTCGACA CAACAGACGG GCACAGCACT ACTTGAAGCA CTCAAGGCAA   121 GCTTTATTGA GGGTTAAGCA GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC AGGCTCAGAT   181 CTGGTCTAAC CAGAGAGACC CAGTACAGTC CGGATGCAGC TCTCGGGCCA TGTGATGAAA   241 TGCTAGGCGG CTGTCAAACC TCCACTCTAA TACTTCTCTC TCCGGGTCAT CCATCCCATG   301 CAGGCTCACA GGGTGTAACA AGCGGGTGTT CTCTCCTTCA TTGGCTTCTT CTACCTTCTC   361 TTGCTCAACT GGTACTAGCT TGTAGCACCA TCCAAAGGTC AGTGGATATC TGATCCCTGG   421 CCCTGGTGTG TAGTTCTGCC AATCAGGGAA GTAGCCTTGT GTGTGGTAGA TCCACAGATC   481 AAGGATATCT TGTCTTCGTT GGGAGTGAAT TAGCCCTTCC AGTCCCCCCT TTTCTTTTAA   541 AAAGTGGCTA AGATCTACAG CTGCCTTGTA AGTCATTGGT CTTAAAGGTA CCTGAGGTGT   601 GACTGGAAAA CCCACCTCCT CCTCCTCTTG TGCTTCTAGC CAGGCACAAT CAGCATTGGT   661 AGCTGCTGTA TTGCTACTTG TGATTGCTCC ATGTTTTTCT AGGTCTCGAC GGTATCGATG   721 CGGGGAGGCG GCCCAAAGGG AGATCCGACT CGTCTGAGGG CGAAGGCGAA GACGCGGAAG   781 AGGCCGCAGA GCCGGCAGCA GGCCGCGGGA AGGAAGGTCC GCTGGATTGA GGGCCGAAGG   841 GACGTAGCAG AAGGACGTCC CGCGCAGAAT CCAGGTGGCA ACACAGGCGA GCAGCCATGG   901 AAAGGACGTC AGCTTCCCCG ACAACACCAC GGAATTGTCA GTGCCCAACA GCCGAGCCCC   961 TGTCCAGCAG CGGGCAAAGG CAGGCGGCGA TGAGTTCCCG CCGTGGCAAT AGGGAGGGGG  1021 AAAGCGAAAG TCCCGGAAAG GAGCTGAACA GGTGAGGCAA TGCCCCCACC AGTTGGGGAT  1081 GCGTCAGCCA ACACAGGTGC AACTACGTCA ACGGTTGTCT GGACAACCGG GTCCTACACA 1141 ACTTCCCTTC AT //
Primer: SV40pro_F Region: SV40pro_ori,EM7pro,Zeo,SV40p(A) Sequence file: RDB15875_A8Cfk.seq
>05964_15875_A8Cf_SV40pro_F_A03_03_ABI24.ab1     1 GCCTGGGTCC CTTAACATTC AGAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC    61 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAGCACGT GTTGACAATT   121 AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA GGTGAGGAAC TAAACCATGG   181 CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA CGTCGCCGGA GCGGTCGAGT   241 TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA GGACGACTTC GCCGGTGTGG   301 TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA CCAGGTGGTG CCGGACAACA   361 CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA CGCCGAGTGG TCGGAGGTCG   421 TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC CGAGATCGGC GAGCAGCCGT   481 GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG CGTGCACTTC GTGGCCGAGG   541 AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG   601 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC   661 TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA   721 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT   781 CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG   841 CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA   901 ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA   961 CATTAATTGC GTTGCGCTCA CTGCCCGCTT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG  1021 CATTAAATGA ATCGGCCAAC GGCGCGGGGG AGAGCGATTT GCGTATTGGG CGCTCTTCGC 1081 TCCTTCGGCT CACTGACTTC GGCTGGCGGC CTCCGGATCC GT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.07.13

GNP_filter3_RDBDEP_html_220713.pl