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CS-CA-MCS

Backbone vector plasmid of lentivirus construct with CAG promoter.

Catalog number RDB05963
Resource name CS-CA-MCS
Clone info. Backbone vector plasmid of lentivirus construct with CAG promoter.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone Plasmid
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Depositor|Developer Miyoshi, Hiroyuki |
Other clones in our bank
External Database
            Reference sequence
              
             
            Sequence (full) RDB05963zzk03.seq provided by the depositor
            Remarks, protocol and/or map (pdf) RDB05963.pdf
            Lentiviral plasmid list. [link]

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
            Ordering Please visit Ordering instruction.[link] 
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            Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
            Remarks Remember that you will be working with samples containing infectious virus.
            提供案内 (日本国内) [open/close]

            提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
            提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (非営利機関による非営利学術研究用)[Word]
            遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
            備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB05963 CS-CA-MCS DNA solution

            Ordering instruction of plasmids [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The CS-CA-MCS was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05963).

            Reference section:

            Further references such as user reports and related articles (go to bottom)

            References and tips

            Featured content

            Featured content Lentivirus Vector (English text)
            Featured content Lentivirus Vector (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15876_A8Cfp1-3.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pAxCA_F1 (Pr0164)
            Region: MCS,PRE
            Sequence file: RDB15876_A8Cfg.seq check
            >05963_15876_3_A8Cf_pAXCA_F1_D01_10_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNCT TCTTTTTCCT ACNGCTCCTG GGCAACGTGC
               61 TGGTTATTGT GCTGTCTCAT CATTTTGGCA AAGAATTGAT TTGATACGTA CGGTTGGGCT
              121 GCAGGAATTC TGCAGATATC CAGCACAGTG GCGGCCGCTC GAGGGATCAA GCTTATCGAT
              181 AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT
              241 CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT
              301 ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG
              361 TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT
              421 GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT
              481 ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG
              541 TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAGCTGA CGTCCTTTCC ATGGCTGCTC
              601 GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC
              661 AATCCAGCGG ANCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT
              721 CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG CCTCCCCGCA TCGATACCGT
              781 CGAGACCTAG AAAAACATGG AGCAATCNCN AGTAGCAATA CAGCAGCTAC CAATGCTGAT
              841 TGTGCCTGGN CTAGAANCAC NAGAGGANGA GGANGNGGGT TTTCCNGTCA CACCTCNNGN
              901 ANCTTTNAGA NCNATGANTT ACNANGCANC TNNAGATCTN NNCCNCTTTT TNAAAGAAAA
              961 GGGGGGNANT GNNNNNGGNT NNTTCNNNCN NNNNNNNNAN NNNNNATNNN TNGNNNNNNN
            1021 NNN
            //
            Primer: CMV_Forward (Pr0016)
            Region: CMV pro.,5' LTR,HIV-1 psi
            Sequence file: RDB15876_A8Cfh.seq check
            >05963_15876_A9HkS_CMV-Forward_D04_04_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NTGGGCGGTA GGCGTGTACG GTGGGAGGTC
               61 TATATAAGCA GCGCGTTTTG CCTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG
              121 GGAGCTCTCT GGCTAACTAG GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT
              181 GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC
              241 CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG TGGCGCCCGA ACAGGGACTT GAAAGCGAAA
              301 GGGAAACCAG AGGAGCTCTC TCGACGCAGG ACTCGGCTTG CTGAAGCGCG CACGGCAAGA
              361 GGCGAGGGGC GGCGACTGGT GAGTACGCCA AAAATTTTGA CTAGCGGAGG CTAGAAGGAG
              421 AGAGATGGGT GCGAGAGCGT CAGTATTAAG CGGGGGAGAA TTAGATCGCG ATGGGAAAAA
              481 ATTCGGTTAA GGCCAGGGGG AAAGAAAAAA TATAAATTAA AACATATAGT ATGGGCAAGC
              541 AGGGAGCTAG AACGATTCGC AGTTAATCCT GGCCTGTTAG AAACATCAGA AGGCTGTAGA
              601 CAAATACTGG GACAGCTACA ACCATCCCTT CAGACAGGAT CAGAAGAACT TAGATCATTA
              661 TATAATACAG TAGCAACCCT CTATTGTGTG CATCAAAGGA TAGAGATAAA AGACACCAAG
              721 GAAGCTTTAN NCAAGATAGA GGAAGAGCAA AACAAAAGTA AGACCACCGC ACAGCANGCG
              781 GCCGCTGATC TTCAGACCTG GAGGAGGAGA TATGANGGNC AATTGGAGAA GTGAATTATA
              841 TAAANTATNA AGTAGTAAAA AATTGNNCCA TTNNGGAGNN NNCNNCCCAC CNNNGGNAAA
              901 NNAGAAANAG TGGTGCNNNN ANNAAAAAAA GAGCANTGGG GAANTNNNGN ANCTTTNTTN
            961 NNNTGGGNTN CNTNGGGNAN NNNNNNNNN
            //
            Primer: RRE-M_R (Pr0651)
            Region: RRE,HIV-1 psi
            Sequence file: RDB15876_A8Cfi.seq check
            >05963_15876_A9HgF_RRE-M_R_C01_07_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNGCT GTTGCGCCTC NNTAGCCCTC NGCAAATTGT TCTGCTGCTG
               61 CACTATACCA GACAATAATT GTCTGGCCTG TACCGTCAGC GTCATTGACG CTGCGCCCAT
              121 AGTGCTTCCT GCTGCTCCCA AGAACCCAAG GAACAAAGCT CCTATTCCCA CTGCTCTTTT
              181 TTCTCTCTGC ACCACTCTTC TCTTTGCCTT GGTGGGTGCT ACTCCTAATG GTTCAATTTT
              241 TACTACTTTA TATTTATATA ATTCACTTCT CCAATTGTCC CTCATATCTC CTCCTCCAGG
              301 TCTGAAGATC AGCGGCCGCT TGCTGTGCGG TGGTCTTACT TTTGTTTTGC TCTTCCTCTA
              361 TCTTGTCTAA AGCTTCCTTG GTGTCTTTTA TCTCTATCCT TTGATGCACA CAATAGAGGG
              421 TTGCTACTGT ATTATATAAT GATCTAAGTT CTTCTGATCC TGTCTGAAGG GATGGTTGTA
              481 GCTGTCCCAG TATTTGTCTA CAGCCTTCTG ATGTTTCTAA CAGGCCAGGA TTAACTGCGA
              541 ATCGTTCTAG CTCCCTGCTT GCCCATACTA TATGTTTTAA TTTATATTTT TTCTTTCCCC
              601 CTGGCCTTAA CCGAATTTTT TCCCATCGCG ATCTAATTCT CCCCCGCTTA ATACTGACGC
              661 TCTCGCACCC ATCTCTCTCC TTCTAGCCTC CGCTAGTCAA AATTTTTGGC GTACTCACCA
              721 GTCGCCGCCC CTCGCCTCTT GCCGTGCGCG CTTCAGCAAG CCGANTCCTG CGTCGAGAGA
              781 GCTCCTCTGG TTTCCCTTTC GCTTTCAAGT CCCTGTTCGG GCGCCACTGC TAGAGATTTT
              841 CCACACNNNC TAAAANGGTC TGANGGANCT CTAGTNACCA GANNCACACA ACANANNGGC
              901 ACACACTACT TNAAGCANTC NAGGNNANCT TTATTGNNGN NTNNNCANNN GGGNNCCNNN
            961 NNTNAGCNNN NNNNNNN
            //
            Primer: env_F (Pr0258)
            Region: cPPT_CTS,CAG pro
            Sequence file: RDB15876_A8Cfj.seq check
            >05963_15876_3_A8Cf_env_F_C01_07_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNTNN NNNNNNNNNN NGGNNCTACA AATGGCAGTA TTCNTCCACA
               61 ATTTTAAAAG AAAAGGGGGG NNTGGGGGGT ACAGTGCAGG GGAAAGAATA GTAGACATAA
              121 TAGCAACAGA CATACAAACT AAAGAATTAC AAAAACAAAT TACAAAAATT CAAAATTTTC
              181 GGGTTTATTA CAGGGACAGC AGAAATTCAC TTTGAATTGG GCTGCAGGAA TCTAGTTATT
              241 AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA TATGGAGTTC CGCGTTACAT
              301 AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA CCCCCGCCCA TTGACGTCAA
              361 TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT CCATTGACGT CAATGGGTGG
              421 AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT GTATCATATG CCAAGTACGC
              481 CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA TTATGCCCAG TACATGACCT
              541 TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT CATCGCTATT ACCATGGTCG
              601 AGGTGAGCCC CACGTTCTGC TTCACTCTCC CCATCTCCCC CCCCTCCCCA CCCCCAATTT
            661 TGTATTTATT TATTTTTTAA TTATTTTGTG CAGCGATGGG GGCGGGGGGG GGGGGNN
            //
            Primer: bGH_rev3 (Pr0885)
            Region: 3'LTR
            Sequence file: RDB15876_A8Cfk.seq check
            >05963_15876_A8Cf_3_bGH_rev3_G04_07_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNGNNNGNN NNNNNNCAGA TGGCTGGCAA CTAGAAGGCA
               61 CAGTCGAGGC TGATCANNNN GTTTAAACGG GCCCTGCTAG AGATTTTCCA CACTGACTAA
              121 AAGGGTCTGA GGGATCTCTA GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA
              181 AGCACTCAAG GCAAGCTTTA TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC
              241 TCCCAGGCTC AGATCTGGTC TAACCAGAGA GACCCAGTAC AGTCCGGATG CAGCTCTCGG
              301 GCCATGTGAT GAAATGCTAG GCGGCTGTCA AACCTCCACT CTAATACTTC TCTCTCCGGG
              361 TCATCCATCC CATGCAGGCT CACAGGGTGT AACAAGCGGG TGTTCTCTCC TTCATTGGCT
              421 TCTTCTACCT TCTCTTGCTC AACTGGTACT AGCTTGTAGC ACCATCCAAA GGTCAGTGGA
              481 TATCTGATCC CTGGCCCTGG TGTGTAGTTC TGCCAATCAG GGAAGTAGCC TTGTGTGTGG
              541 TAGATCCACA GATCAAGGAT ATCTTGTCTT CGTTGGGAGT GAATTANCCC TTCCAGTCCC
              601 CCCTTTTCTT TTAAAAAGTG GCTAAGATCT ACAGCTGCCT TGTAAGTCAT TGGTCTTAAA
              661 GGTACCTGAG GTGTGACTGG AAAACCCACC TCCTCCTCCT CTTGTGCTTC TAGCNNGGCA
              721 CAATCAGCAT TGGTAGCTGC TGTATTGCTA CTTGTGATTG CTCCATGTTT TTCTAGGTCT
              781 CGACNGTATC GATGCNGGGA NGCGGCCCAA AGGGAGATNC GANTCGTCTG NNGGCGANNN
              841 NAAGANNCNN AANANGCCNC NNANCNNGCA NNNNNNCNCG NGAANGNANN NNCGCTGGNN
              901 TGANGGNCNA NNGNACNTAN NNNANNNNNT NCCGCGCANA NNNNANNTGG NNNCACNNNN
            961 NANCNNNNNT NGNAAAGNAN NNNNNCTTNN CCNNNANNNN NNNNNN
            //
            Primer: SV40pro_F (Pr0110)
            Region: Zeo,SV40 pA
            Sequence file: RDB15876_A8Cfl.seq check
            >05963_15876_A8Cf_3_SV40pro_F_G01_07_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNTT TTTTGGANGC CTAGGCTTTT
               61 GNNNAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAGCA CGTGTTGACA
              121 ATTAATCATC GGCATAGTAT ATCGGCATAG TATAATACGA CAAGGTGAGG AACTAAACCA
              181 TGGCCAAGTT GACCAGTGCC GTTCCGGTGC TCACCGCGCG CGACGTCGCC GGAGCGGTCG
              241 AGTTCTGGAC CGACCGGCTC GGGTTCTCCC GGGACTTCGT GGAGGACGAC TTCGCCGGTG
              301 TGGTCCGGGA CGACGTGACC CTGTTCATCA GCGCGGTCCA GGACCAGGTG GTGCCGGACA
              361 ACACCCTGGC CTGGGTGTGG GTGCGCGGCC TGGACGAGCT GTACGCCGAG TGGTCGGAGG
              421 TCGTGTCCAC GAACTTCCGG GACGCCTCCG GGCCGGCCAT GACCGAGATC GGCGAGCAGC
              481 CGTGGGGGCG GGAGTTCGCC CTGCGCGACC CGGCCGGCAA CTGCGTGCAC TTCGTGGCCG
              541 AGGAGCAGGA CTGACACGTG CTACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT
              601 TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA
              661 TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA
              721 GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT ANTTGTGGTT
              781 TGTCCAAACT CATCAATGTA TCTNATCATG TCTGTATACC GTCGACCTCT ANCTANAGCT
              841 TGGCGTAATC ATGGTCATAG CTGTTNNNNG TGTGAAATTG TNATCCGCTC ACANTTCCAC
              901 ACAANNTACN ANCNNNAANN CNNNAAAGTG NAANGNCNGN NGNGNCNAAN GANTGANCTA
            961 NNNCNNNTTA ANNTGCGNTN NNGCTCNNNG CCNNNNNTTT NNNNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            user_report Kobayashi, N., The microRNA cluster C19MC confers differentiation potential into trophoblast lineages upon human pluripotent stem cells. Nat. Commun. 13 (1): 3071 (2022). PMID 35654791.
            user_report Ikeda, A., Colorectal Cancer-Derived CAT1-Positive Extracellular Vesicles Alter Nitric Oxide Metabolism in Endothelial Cells and Promote Angiogenesis. Mol. Cancer Res. 19 (5): 834-846 (2021). PMID 33579815.
            user_report Sampilvanjil, A., Cigarette smoke extract induces ferroptosis in vascular smooth muscle cells. Am. J. Physiol. Heart Circ. Physiol. 318 (3): H508-H518 (2020). PMID 31975626.
            user_report Ouwendijk, W.J.D., Varicella-zoster virus VLT-ORF63 fusion transcript induces broad viral gene expression during reactivation from neuronal latency. Nat. Commun. 11 (1): 6324 (2020). PMID 33303747.
            user_report Frazier, A.E., Fatal Perinatal Mitochondrial Cardiac Failure Caused by Recurrent De Novo Duplications in the ATAD3 Locus. Med (N Y) 2 (1): 49-73 (2020) PMID 33575671.
            user_report Borna, N.N., Mitochondrial ribosomal protein PTCD3 mutations cause oxidative phosphorylation defects with Leigh syndrome. Neurogenetics 20 (1): 9-25 (2019). PMID 30607703.
            user_report Mitani, A., Characterization of doxycycline-dependent inducible Simian Virus 40 large T antigen immortalized human conjunctival epithelial cell line. PLoS One 14 (9): e0222454 (2019). PMID 31509592.
            user_report Takahashi, S., Loss of p57 KIP2 expression confers resistance to contact inhibition in human androgenetic trophoblast stem cells. Proc. Natl. Acad. Sci. U.S.A. pii: 201916019 (2019). PMID 31792181.
            user_report Karasawa, T., Saturated Fatty Acids Undergo Intracellular Crystallization and Activate the NLRP3 Inflammasome in Macrophages Arterioscler. Thromb. Vasc. Biol. 38 (4): 744-756 (2018). PMID 29437575.
            user_report Kawashima, A., ARIH2 Ubiquitinates NLRP3 and Negatively Regulates NLRP3 Inflammasome Activation in Macrophages. J. Immunol. 199 (10): 3614-3622 (2017). PMID 29021376.
            user_report Kohda, M., A Comprehensive Genomic Analysis Reveals the Genetic Landscape of Mitochondrial Respiratory Chain Complex Deficiencies. PLoS Genet. 12 (1): e1005679 (2016). PMID 26741492.
            user_report Kishita, Y., Intra-mitochondrial Methylation Deficiency Due to Mutations in SLC25A26. Am. J. Hum. Genet. 97 (5): 761-768 (2015). PMID 26522469.
            user_report Okada, K., Enhanced flexibility of place discrimination learning by targeting striatal cholinergic interneurons. Nat. Commun., 5: 3778 (2014). PMID 24797209.
            user_report Nanba, D., Actin filament dynamics impacts keratinocyte stem cell maintenance. EMBO Mol Med., 5 (4): 640-653 (2013). PMID 23554171.
            user_report Matsushita, N., Doxycycline-dependent inducible and reversible RNA interference mediated by a single lentivirus vector. Biosci. Biotechnol. Biochem., 77 (4): 776-781 (2013). PMID 23563548.
            user_report Tang, H., CD134 is a cellular receptor specific for human herpesvirus-6B entry. Proc. Natl. Acad. Sci. U.S.A., 110 (22): 9096-9099 (2013). PMID 23674671.
            user_report Yaguchi, M., Characterization of the properties of seven promoters in the motor cortex of rats and monkeys after lentiviral vector-mediated gene transfer. Hum. Gene Ther. Methods, 24 (6): 333-344 (2013). PMID 23964981.
            user_report Tamura, K., A glass-coated tungsten microelectrode enclosing optical fibers for optogenetic exploration in primate deep brain structures. J. Neurosci. Methods, 211 (1): 49-57 (2012). PMID 22971353.
            user_report Fujita, K., Effects of a brain-engraftable microglial cell line expressing anti-prion scFv antibodies on survival times of mice infected with scrapie prions. Cell. Mol. Neurobiol. 31 (7): 999-1008 (2011). PMID 21516351.
            user_report Tsubota, T., Optogenetic manipulation of cerebellar purkinje cell activity in vivo. PLoS One 6 (8): e22400 (2011). PMID 21850224.
            user_report Nishie T., Beta4-galactosyltransferase-5 is a lactosylceramide synthase essential for mouse extra-embryonic development. Glycobiology., 20, 1311-1322 (2010). PMID 20574042.
            user_report Ohashi Y., A bicistronic lentiviral vector-based method for differential transsynaptic tracing of neural circuits. Mol. Cell. Neurosci. 46 (1): 136-147. PMID 20816792.
            user_report Inuzuka T., Transcription factor 8 activates R-Ras to regulate angiogenesis. Biochem. Biophys. Res. Commun., 379, 510-513 (2009). PMID 19116136.

            2023.05.22

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