Resource data sheet
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CS-CA-MCS

Shuttlevector to generate Lentiviral vector.

Catalog number RDB05963
Resource name CS-CA-MCS
Clone info. Shuttlevector to generate Lentiviral vector.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone Plasmid
Selectable markers Amp^r
Gene/insert name Vector
Originator Miyoshi, Hiroyuki |

Distribution information

CLICK here for plasmid list and map. [link]
Forms for plasmid request [in Japanese] [in English]
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
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Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB05963_A3Frp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 RDB05963_A3Fra.seq
>05963_05963_A3Fr_CMV-Forward_A07_01.ab1
    1 AGTTTACTCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGCGCGTTT TGCCTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT
  121 CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG
  181 TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT
  241 CAGTGTGGAA AATCTCTAGC AGTGGCGCCC GAACAGGGAC TTGAAAGCGA AAGGGAAACC
  301 AGAGGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG
  361 GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG
  421 GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT
  481 AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT
  541 AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT
  601 GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC
  661 AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT
  721 AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCGCTGA
  781 TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGGA GAAGTGAAAT TATATAAATA
  841 TAAAGTAGTA AAAATTTGAA CTCATTTAGG AGTAGCCACC CCCACCCAAA GGCAAAAGAG
  901 AAAGAAGTGG GTGCAGAAGA GAAAAAAAAG AAGCAGTGGG GAAATAGGAG CTTTGTTCCT
  961 TGGGTTCTTT GGGGAGCAGC AGAGCACTAT GGGGGCGCAG CCGTTCATGG ACGCTGACGG
1021 GTACCAGGCC AGAACAATTA TTGTGCTGAT AGGTGCAGCA GCCGGAACAA TTTC
//
Primer: HIVgp_R1 RDB05963_A3Frb.seq
>05963_05963_A3Fr_BGHrev_G02_20.ab1
    1 GTTACGGACT GCTAGAGATT TTCCACACTG ACTAAAAGGG TCTGAGGGAT CTCTAGTTAC
   61 CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC TCAAGGCAAG CTTTATTGAG
  121 GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA GGCTCAGATC TGGTCTAACC
  181 AGAGAGACCC AGTACAGTCC GGATGCAGCT CTCGGGCCAT GTGATGAAAT GCTAGGCGGC
  241 TGTCAAACCT CCACTCTAAT ACTTCTCTCT CCGGGTCATC CATCCCATGC AGGCTCACAG
  301 GGTGTAACAA GCGGGTGTTC TCTCCTTCAT TGGCTTCTTC TACCTTCTCT TGCTCAACTG
  361 GTACTAGCTT GTAGCACCAT CCAAAGGTCA GTGGATATCT GATCCCTGGC CCTGGTGTGT
  421 AGTTCTGCCA ATCAGGGAAG TAGCCTTGTG TGTGGTAGAT CCACAGATCA AGGATATCTT
  481 GTCTTCGTTG GGAGTGAATT AGCCCTTCCA GTCCCCCCTT TTCTTTTAAA AAGTGGCTAA
  541 GATCTACAGC TGCCTTGTAA GTCATTGGTC TTAAAGGTAC CTGAGGTGTG ACTGGAAAAC
  601 CCACCTCCTC CTCCTCTTGT GCTTCTAGCC AGGCACAATC AGCATTGGTA GCTGCTGTAT
  661 TGCTACTTGT GATTGCTCCA TGTTTTTCTA GGTCTCGACG GTATCGATGC GGGGAGGCGG
  721 CCCAAAGGGA GATCCGACTC GTCTGAGGGC GAAGGCGAAG ACGCGGAAGA GGCCGCAGAG
  781 CCGGCAGCAG GCCGCGGGAA GGAAGGTCCG CTGGATTGAG GGCCGAAGGG ACGTAGCAGA
  841 AGGACGTCCC GCGCAGAATC CAGGTGGCAA CACAGGCGAG CAGCCATGGA AAGGACGTCA
  901 GCTTCCCCGA CAACACCACG GAATTGTCAG TGCCCAACAG CCGAGCCCCT GTCCAGCAGC
  961 GGGCAAGGCA GGCGGCGATG AGTTCCGCCG TGGCAATAGG GAGGGGGAAA GCGAAAGTCC
 1021 CGGGAAAGAG CTGACAGGTG TGGCAATGCC CCACCCAGTG GGGGGTGCGT CAGCAAACAC
 1081 AGTTGCACAC CACGGCCACG TGGCTGGACA CGACACACTC CTCATAAGAG ACAGCCAACA
1141 GAATTTATAC AGGGAGGGAA GAAAAATGTG
//
Primer: BGH_rev RDB05963_A3Frc.seq
>05963_05963_A3Fr_pAxCA_F1_E02_14.ab1
    1 GGATATGTCT CATGTTCATG CCTTCTTCTT TTTCCTACAG CTCCTGGGCA ACGTGCTGGT
   61 TATTGTGCTG TCTCATCATT TTGGCAAAGA ATTGATTTGA TACGTACGGT TGGGCTGCAG
  121 GAATTCTGCA GATATCCAGC ACAGTGGCGG CCGCTCGAGG GATCAAGCTT ATCGATAATC
  181 AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT
  241 TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG
  301 CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC
  361 CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT
  421 GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG
  481 CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG
  541 GCACTGACAA TTCCGTGGTG TTGTCGGGGA AGCTGACGTC CTTTCCATGG CTGCTCGCCT
  601 GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC
  661 CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC
  721 TTCGCCCTCA GACGAGTCGG ATCTCCCTTT GGGCCGCCTC CCCGCATCGA TACCGTCGAG
  781 ACCTAGAAAA ACATGGAGCA ATCACAAGTA GCAATACAGC AGCTACCAAT GCTGATTGTG
  841 CCTGGCTAGA AGCACAAGAG GAGGAGGAGG TGGGTTTTCC AGTCACACCT CAGGTACCTT
  901 TAAGACCAAT GACTTACAAA GGCAGCTGTA GATCTTAGCC ACTTTTTAAA AGAAAAAGGG
  961 GGGACTGGAA AGGCTAATTC ACTCCCAACG AAGACAAGAT ATCCTTGATC TGTGGATCTA
 1021 CCACACACAA GCTACTTCCC TGATTGGCAG AACTACACAC CAGGGCCAGG GATCAGATAT
 1081 CCACTGACCC TTTGCATGGT GCTACAGCTA GTACCAGTTG AGCCAGAGAT GGTAGAGAGG
1141 CATTGAGAGA GAACACCCCG CCTTTGTTAC CACCCCTGTG TGTGAAGA
//
Primer: SV40pro_F RDB05963_A3Frd.seq
>05963_05963_A3Fr_SV40pro_F_H02_23.ab1
    1 GGCGGGGTTC GTCCGCTCGA TGTCTGACAT CAGAGTAGTG AGGAGGCTTT TTTGGAGGCC
   61 TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAGCACG
  121 TGTTGACAAT TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACA AGGTGAGGAA
  181 CTAAACCATG GCCAAGTTGA CCAGTGCCGT TCCGGTGCTC ACCGCGCGCG ACGTCGCCGG
  241 AGCGGTCGAG TTCTGGACCG ACCGGCTCGG GTTCTCCCGG GACTTCGTGG AGGACGACTT
  301 CGCCGGTGTG GTCCGGGACG ACGTGACCCT GTTCATCAGC GCGGTCCAGG ACCAGGTGGT
  361 GCCGGACAAC ACCCTGGCCT GGGTGTGGGT GCGCGGCCTG GACGAGCTGT ACGCCGAGTG
  421 GTCGGAGGTC GTGTCCACGA ACTTCCGGGA CGCCTCCGGG CCGGCCATGA CCGAGATCGG
  481 CGAGCAGCCG TGGGGGCGGG AGTTCGCCCT GCGCGACCCG GCCGGCAACT GCGTGCACTT
  541 CGTGGCCGAG GAGCAGGACT GACACGTGCT ACGAGATTTC GATTCCACCG CCGCCTTCTA
  601 TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG
  661 GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA
  721 CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG
  781 TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG
  841 CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC
  901 AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT
  961 GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC
 1021 GTGCCAGCTG CATTAATGGA ATCGGGCCAA CGCGCGGGGA GAGGCGGATT GCGTATTGGG
 1081 GGCGCTCTTC CGCTTCTCGC CTCACTGACT CGCTGCGCTT CGGTTCGTTT CGGCTGGCGC
1141 CGAGCCGGTA TCAAGCTCAG TCAAGCGGTT AATTACCGGG GCTTATATTC
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

References

user report Kohda, M., A Comprehensive Genomic Analysis Reveals the Genetic Landscape of Mitochondrial Respiratory Chain Complex Deficiencies. PLoS Genet. 12 (1): e1005679 (2016). PMID 26741492.
user report Kishita, Y., Intra-mitochondrial Methylation Deficiency Due to Mutations in SLC25A26. Am. J. Hum. Genet. 97 (5): 761-768 (2015). PMID 26522469.
user report Okada, K., Enhanced flexibility of place discrimination learning by targeting striatal cholinergic interneurons. Nat. Commun., 5: 3778 (2014). PMID 24797209.
user report Nanba, D., Actin filament dynamics impacts keratinocyte stem cell maintenance. EMBO Mol Med., 5 (4): 640-653 (2013). PMID 23554171.
user report Matsushita, N., Doxycycline-dependent inducible and reversible RNA interference mediated by a single lentivirus vector. Biosci. Biotechnol. Biochem., 77 (4): 776-781 (2013). PMID 23563548.
user report Tang, H., CD134 is a cellular receptor specific for human herpesvirus-6B entry. Proc. Natl. Acad. Sci. U.S.A., 110 (22): 9096-9099 (2013). PMID 23674671.
user report Yaguchi, M., Characterization of the properties of seven promoters in the motor cortex of rats and monkeys after lentiviral vector-mediated gene transfer. Hum. Gene Ther. Methods, 24 (6): 333-344 (2013). PMID 23964981.
user report Tamura, K., A glass-coated tungsten microelectrode enclosing optical fibers for optogenetic exploration in primate deep brain structures. J. Neurosci. Methods, 211 (1): 49-57 (2012). PMID 22971353.
user report Fujita, K., Effects of a brain-engraftable microglial cell line expressing anti-prion scFv antibodies on survival times of mice infected with scrapie prions. Cell. Mol. Neurobiol. 31 (7): 999-1008 (2011). PMID 21516351.
user report Tsubota, T., Optogenetic manipulation of cerebellar purkinje cell activity in vivo. PLoS One 6 (8): e22400 (2011). PMID 21850224.
user report Nishie T., Beta4-galactosyltransferase-5 is a lactosylceramide synthase essential for mouse extra-embryonic development. Glycobiology., 20, 1311-1322 (2010). PMID 20574042.
user report Ohashi Y., A bicistronic lentiviral vector-based method for differential transsynaptic tracing of neural circuits. Mol. Cell. Neurosci. 46 (1): 136-147. PMID 20816792.

2017.10.23

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