Resource data sheet
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CS-CA-MCS

Backbone vector of lentivirus construct with CAG promoter.

Catalog number RDB05963
Resource name CS-CA-MCS
Clone info. Backbone vector of lentivirus construct with CAG promoter.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone Plasmid
Selectable markers Amp^r (E. coli); Zeo^r (plasmid transfection of mammalian cells).
Gene/insert name Vector
Originator Miyoshi, Hiroyuki |

Distribution information

CLICK here for plasmid list and map. [link]
Forms for plasmid request [in Japanese] [in English]
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
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Remarks Remember that you will be working with samples containing infectious virus.
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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15876_A8Cfp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 RDB15876_A8Cfa.seq
>05963_15876_3_A8Cf_pAXCA_F_D01_10_ABI24.ab1
    1 TGTGCCCTCC CAATGGTTTT CATGCCTTCT TCTTTTTCCT ACAGCTCCTG GGCAACGTGC
   61 TGGTTATTGT GCTGTCTCAT CATTTTGGCA AAGAATTGAT TTGATACGTA CGGTTGGGCT
  121 GCAGGAATTC TGCAGATATC CAGCACAGTG GCGGCCGCTC GAGGGATCAA GCTTATCGAT
  181 AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT
  241 CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT
  301 ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG
  361 TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT
  421 GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT
  481 ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG
  541 TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAGCTGA CGTCCTTTCC ATGGCTGCTC
  601 GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC
  661 AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT
  721 CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG CCTCCCCGCA TCGATACCGT
  781 CGAGACCTAG AAAAACATGG AGCAATCACA AGTAGCAATA CAGCAGCTAC CAATGCTGAT
  841 TGTGCCTGGG CTAGAAGCAC AAGAGGAGGA GGAGGTGGGT TTTCCAGTCA CACCTCAGGT
  901 ACCTTTAAGA CCAATGACTT ACAAGGCAGC TGTAGATCTT AGCCACTTTT TAAAAGAAAA
  961 GGGGGGGACT GGGAAGGGCT AATTCACTCC CAACGAAGAC AAGATATCCT TTGATCTGTG
 1021 GATCTACCAC ACACAGGCTA CTTCCCCTGA TTGGCAGACT ACACAACCAG GCCAAGGGAT
 1081 CAGGATATCC CACTGAACTT GAATGTGCCT ACAGGCTTAG TTACTAAGGT TGAGAAGTCC
1141 AAGGGAAGAG
//
Primer: HIVgp_R1 RDB15876_A8Cfb.seq
>05963_15876_A8Cf_3_HIVgp_R1_C01_03_ABI08.ab1
    1 GGAGCCATCC CTAATTTCAA CTGGCCTCTG TTTTTCTCTC TGGCACCCAC TCTTCTCTTT
   61 GCCTTGGTGG GTGCTACTCC TAATGGTTCA ATTTTTACTA CTTTATATTT ATATAATTCA
  121 CTTCTCCAAT TGTCCCTCAT ATCTCCTCCT CCAGGTCTGA AGATCAGCGG CCGCTTGCTG
  181 TGCGGTGGTC TTACTTTTGT TTTGCTCTTC CTCTATCTTG TCTAAAGCTT CCTTGGTGTC
  241 TTTTATCTCT ATCCTTTGAT GCACACAATA GAGGGTTGCT ACTGTATTAT ATAATGATCT
  301 AAGTTCTTCT GATCCTGTCT GAAGGGATGG TTGTAGCTGT CCCAGTATTT GTCTACAGCC
  361 TTCTGATGTT TCTAACAGGC CAGGATTAAC TGCGAATCGT TCTAGCTCCC TGCTTGCCCA
  421 TACTATATGT TTTAATTTAT ATTTTTTCTT TCCCCCTGGC CTTAACCGAA TTTTTTCCCA
  481 TCGCGATCTA ATTCTCCCCC GCTTAATACT GACGCTCTCG CACCCATCTC TCTCCTTCTA
  541 GCCTCCGCTA GTCAAAATTT TTGGCGTACT CACCAGTCGC CGCCCCTCGC CTCTTGCCGT
  601 GCGCGCTTCA GCAAGCCGAG TCCTGCGTCG AGAGAGCTCC TCTGGTTTCC CTTTCGCTTT
  661 CAAGTCCCTG TTCGGGCGCC ACTGCTAGAG ATTTTCCACA CTGACTAAAA GGGTCTGAGG
  721 GATCTCTAGT TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG CACTCAAAGG
  781 CAAGCTTTAT TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT CTCAGGCTCA
  841 GATCTGGTCT AACCAGAAGA GACCCAGTAC AGGGCAAAAA CGCGCTGCTT ATATAGACCT
  901 CCCACCGTAC ACGCCTACCG CCCATTTGCG TCATGGGGGC GGGAGTTGTT ACGACATTTT
  961 GGAAAGTCCC GTTGATTTTG GGTGCCAAAA CAAACTCCCA TTGACGTCAA ATGGGTGGGA
 1021 AGAACTTGGG AAATCCCCCG TGAAGTCAAA CCGGCTATCC ACGCCCATTG GATGGTACTG
 1081 CCAAAAACCG CATCACATGG TAAATTAGCC GGATGACTAT TACGTAGAAT GTACTGCTCA
1141 AAGTAGGAAA AAGTCCTCCA ATATAAAGGG TCAT
//
Primer: BGH_rev RDB15876_A8Cfc.seq
>05963_15876_3_A8Cf_BGH_rev_E01_13_ABI24.ab1
    1 GATAGGGGGA CTGCTAGAGA TTTTCCACAC TGACTAAAGG GTCTGAGGGA TCTCTAGTTA
   61 CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA CTCAAGGCAA GCTTTATTGA
  121 GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC AGGCTCAGAT CTGGTCTAAC
  181 CAGAGAGACC CAGTACAGTC CGGATGCAGC TCTCGGGCCA TGTGATGAAA TGCTAGGCGG
  241 CTGTCAAACC TCCACTCTAA TACTTCTCTC TCCGGGTCAT CCATCCCATG CAGGCTCACA
  301 GGGTGTAACA AGCGGGTGTT CTCTCCTTCA TTGGCTTCTT CTACCTTCTC TTGCTCAACT
  361 GGTACTAGCT TGTAGCACCA TCCAAAGGTC AGTGGATATC TGATCCCTGG CCCTGGTGTG
  421 TAGTTCTGCC AATCAGGGAA GTAGCCTTGT GTGTGGTAGA TCCACAGATC AAGGATATCT
  481 TGTCTTCGTT GGGAGTGAAT TAGCCCTTCC AGTCCCCCCT TTTCTTTTAA AAAGTGGCTA
  541 AGATCTACAG CTGCCTTGTA AGTCATTGGT CTTAAAGGTA CCTGAGGTGT GACTGGAAAA
  601 CCCACCTCCT CCTCCTCTTG TGCTTCTAGC CAGGCACAAT CAGCATTGGT AGCTGCTGTA
  661 TTGCTACTTG TGATTGCTCC ATGTTTTTCT AGGTCTCGAC GGTATCGATG CGGGGAGGCG
  721 GCCCAAAGGG AGATCCGACT CGTCTGAGGG CGAAGGCGAA GACGCGGAAG AGGCCGCAGA
  781 GCCGGCAGCA GGCCGCGGGA AGGAAGGTCC GCTGGATTGA GGGCCGAAGG GACGTAGCAG
  841 AAGGACGTCC CGCGCAGAAT CCAGGTGGCA ACACAGGCGA GCAGCCATGG AAAGGACGTC
  901 AGCTTCCCCG ACAACACCAC GGAATTGTCA GTGCCCAACA GCCGAGCCCC TGTCCAGCAG
  961 CGGGCAAGCA GCGGCGATGA GTTTCCGCCG TGGCAATAGG GAGGGGGAAA GCGAAAGTCC
 1021 CGAAAGGAGC TGACAGTTGT TGCATGCCCT CACCAGTGGG GGATGCGTCA GCCAACACAG
1081 TGGCAACTCA GCAACGTGCT GAACAACCGG ATCTACAAAC TTCCCTCATT AAA
//
Primer: SV40pro_F RDB15876_A8Cfd.seq
>05963_15876_A8Cf_3_SV40pro_F_G01_07_ABI08.ab1
    1 ATTGGTCCCG GTAACAAATC AAGGAAGTAG TGAGGAGCTT TTTTGGAGGC CTAGGCTTTT
   61 GCAAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAGCA CGTGTTGACA
  121 ATTAATCATC GGCATAGTAT ATCGGCATAG TATAATACGA CAAGGTGAGG AACTAAACCA
  181 TGGCCAAGTT GACCAGTGCC GTTCCGGTGC TCACCGCGCG CGACGTCGCC GGAGCGGTCG
  241 AGTTCTGGAC CGACCGGCTC GGGTTCTCCC GGGACTTCGT GGAGGACGAC TTCGCCGGTG
  301 TGGTCCGGGA CGACGTGACC CTGTTCATCA GCGCGGTCCA GGACCAGGTG GTGCCGGACA
  361 ACACCCTGGC CTGGGTGTGG GTGCGCGGCC TGGACGAGCT GTACGCCGAG TGGTCGGAGG
  421 TCGTGTCCAC GAACTTCCGG GACGCCTCCG GGCCGGCCAT GACCGAGATC GGCGAGCAGC
  481 CGTGGGGGCG GGAGTTCGCC CTGCGCGACC CGGCCGGCAA CTGCGTGCAC TTCGTGGCCG
  541 AGGAGCAGGA CTGACACGTG CTACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT
  601 TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA
  661 TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA
  721 GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT
  781 TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC GTCGACCTCT AGCTAGAGCT
  841 TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC
  901 ACAACATACG AGCCGGAAGG CATAAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA
  961 ACTCACATTA AATTGCGTTT GCGCTCACTG CCCGGCTTTT CCAGTTCGGA AACCCTGGTC
 1021 GTGGCCAGCT GCCATTAAAT GAATCGGGCC AACGCGCGGG GGAGAGGCGG TTTGCGTATG
 1081 GGGCGGCTCC TCGGCACGTC GCTTCACCTG GACCTCGCCT TGGCGGCCCT TCCGGGTTCC
1141 GGTTCTCG
//

Please visit Sequencing and PCR primers for primer information.

References and tips

Electronic file

References

user report Karasawa, T., Saturated Fatty Acids Undergo Intracellular Crystallization and Activate the NLRP3 Inflammasome in Macrophages Arterioscler Thromb Vasc Biol. 2018 Feb 8. pii: ATVBAHA.117.310581. doi: 10.1161/ATVBAHA.117.310581. [Epub ahead of print] PMID 29437575.
user report Kawashima, A., ARIH2 Ubiquitinates NLRP3 and Negatively Regulates NLRP3 Inflammasome Activation in Macrophages. J. Immunol. 199 (10): 3614-3622 (2017). PMID 29021376.
user report Kohda, M., A Comprehensive Genomic Analysis Reveals the Genetic Landscape of Mitochondrial Respiratory Chain Complex Deficiencies. PLoS Genet. 12 (1): e1005679 (2016). PMID 26741492.
user report Kishita, Y., Intra-mitochondrial Methylation Deficiency Due to Mutations in SLC25A26. Am. J. Hum. Genet. 97 (5): 761-768 (2015). PMID 26522469.
user report Okada, K., Enhanced flexibility of place discrimination learning by targeting striatal cholinergic interneurons. Nat. Commun., 5: 3778 (2014). PMID 24797209.
user report Nanba, D., Actin filament dynamics impacts keratinocyte stem cell maintenance. EMBO Mol Med., 5 (4): 640-653 (2013). PMID 23554171.
user report Matsushita, N., Doxycycline-dependent inducible and reversible RNA interference mediated by a single lentivirus vector. Biosci. Biotechnol. Biochem., 77 (4): 776-781 (2013). PMID 23563548.
user report Yaguchi, M., Characterization of the properties of seven promoters in the motor cortex of rats and monkeys after lentiviral vector-mediated gene transfer. Hum. Gene Ther. Methods, 24 (6): 333-344 (2013). PMID 23964981.
user report Fujita, K., Effects of a brain-engraftable microglial cell line expressing anti-prion scFv antibodies on survival times of mice infected with scrapie prions. Cell. Mol. Neurobiol. 31 (7): 999-1008 (2011). PMID 21516351.
user report Tsubota, T., Optogenetic manipulation of cerebellar purkinje cell activity in vivo. PLoS One 6 (8): e22400 (2011). PMID 21850224.
user report Nishie T., Beta4-galactosyltransferase-5 is a lactosylceramide synthase essential for mouse extra-embryonic development. Glycobiology., 20, 1311-1322 (2010). PMID 20574042.
user report Ohashi Y., A bicistronic lentiviral vector-based method for differential transsynaptic tracing of neural circuits. Mol. Cell. Neurosci. 46 (1): 136-147. PMID 20816792.

2018.04.05

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