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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CS-CA-MCS

Backbone vector plasmid of lentivirus construct with CAG promoter.

Catalog number RDB05963
Resource name CS-CA-MCS
Clone info. Backbone vector plasmid of lentivirus construct with CAG promoter.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone Plasmid
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Depositor|Developer Miyoshi, Hiroyuki |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering Please visit Ordering instruction.[link] 
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Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Remarks Remember that you will be working with samples containing infectious virus.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB05963 CS-CA-MCS DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB05963z.seq
Electronic file Remarks, protocol and/or map (pdf) RDB05963.pdf

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15876_A8Cfp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Region: MCS,WPRE
Sequence file: RDB15876_A8Cfa.seq check
>05963_15876_3_A8Cf_pAXCA_F_D01_10_ABI24.ab1
    1 TGTGCCCTCC CAATGGTTTT CATGCCTTCT TCTTTTTCCT ACAGCTCCTG GGCAACGTGC
   61 TGGTTATTGT GCTGTCTCAT CATTTTGGCA AAGAATTGAT TTGATACGTA CGGTTGGGCT
  121 GCAGGAATTC TGCAGATATC CAGCACAGTG GCGGCCGCTC GAGGGATCAA GCTTATCGAT
  181 AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT
  241 CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT
  301 ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG
  361 TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT
  421 GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT
  481 ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG
  541 TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAGCTGA CGTCCTTTCC ATGGCTGCTC
  601 GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC
  661 AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT
  721 CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG CCTCCCCGCA TCGATACCGT
  781 CGAGACCTAG AAAAACATGG AGCAATCACA AGTAGCAATA CAGCAGCTAC CAATGCTGAT
  841 TGTGCCTGGG CTAGAAGCAC AAGAGGAGGA GGAGGTGGGT TTTCCAGTCA CACCTCAGGT
  901 ACCTTTAAGA CCAATGACTT ACAAGGCAGC TGTAGATCTT AGCCACTTTT TAAAAGAAAA
  961 GGGGGGGACT GGGAAGGGCT AATTCACTCC CAACGAAGAC AAGATATCCT TTGATCTGTG
 1021 GATCTACCAC ACACAGGCTA CTTCCCCTGA TTGGCAGACT ACACAACCAG GCCAAGGGAT
 1081 CAGGATATCC CACTGAACTT GAATGTGCCT ACAGGCTTAG TTACTAAGGT TGAGAAGTCC
1141 AAGGGAAGAG
//
Primer: CMV_Forward
Region: CMV pro.,5'LTR,HIV-1 psi
Sequence file: RDB15876_A8Cfb.seq check
>05963_15876_A9HgF_CMV-Forward_D04_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NTGGGCGGTA GGCGTGTACG GTGGGAGGTC
   61 TATATAAGCA GCGCGTTTTG CCTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG
  121 GGAGCTCTCT GGCTAACTAG GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT
  181 GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC
  241 CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG TGGCGCCCGA ACAGGGACTT GAAAGCGAAA
  301 GGGAAACCAG AGGAGCTCTC TCGACGCAGG ACTCGGCTTG CTGAAGCGCG CACGGCAAGA
  361 GGCGAGGGGC GGCGACTGGT GAGTACGCCA AAAATTTTGA CTAGCGGAGG CTAGAAGGAG
  421 AGAGATGGGT GCGAGAGCGT CAGTATTAAG CGGGGGAGAA TTAGATCGCG ATGGGAAAAA
  481 ATTCGGTTAA GGCCAGGGGG AAAGAAAAAA TATAAATTAA AACATATAGT ATGGGCAAGC
  541 AGGGAGCTAG AACGATTCGC AGTTAATCCT GGCCTGTTAG AAACATCAGA AGGCTGTAGA
  601 CAAATACTGG GACAGCTACA ACCATCCCTT CAGACAGGAT CAGAAGAACT TAGATCATTA
  661 TATAATACAG TAGCAACCCT CTATTGTGTG CATCAAAGGA TAGAGATAAA AGACACCAAG
  721 GAAGCTTTAN NCAAGATAGA GGAAGAGCAA AACAAAAGTA AGACCACCGC ACAGCANGCG
  781 GCCGCTGATC TTCAGACCTG GAGGAGGAGA TATGANGGNC AATTGGAGAA GTGAATTATA
  841 TAAANTATNA AGTAGTAAAA AATTGNNCCA TTNNGGAGNN NNCNNCCCAC CNNNGGNAAA
  901 NNAGAAANAG TGGTGCNNNN ANNAAAAAAA GAGCANTGGG GAANTNNNGN ANCTTTNTTN
961 NNNTGGGNTN CNTNGGGNAN NNNNNNNNN
//
Primer: BGH_rev
Region: bGHP(A),3'LTR
Sequence file: RDB15876_A8Cfc.seq check
>05963_15876_3_A8Cf_BGH_rev_E01_13_ABI24.ab1
    1 GATAGGGGGA CTGCTAGAGA TTTTCCACAC TGACTAAAGG GTCTGAGGGA TCTCTAGTTA
   61 CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA CTCAAGGCAA GCTTTATTGA
  121 GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC AGGCTCAGAT CTGGTCTAAC
  181 CAGAGAGACC CAGTACAGTC CGGATGCAGC TCTCGGGCCA TGTGATGAAA TGCTAGGCGG
  241 CTGTCAAACC TCCACTCTAA TACTTCTCTC TCCGGGTCAT CCATCCCATG CAGGCTCACA
  301 GGGTGTAACA AGCGGGTGTT CTCTCCTTCA TTGGCTTCTT CTACCTTCTC TTGCTCAACT
  361 GGTACTAGCT TGTAGCACCA TCCAAAGGTC AGTGGATATC TGATCCCTGG CCCTGGTGTG
  421 TAGTTCTGCC AATCAGGGAA GTAGCCTTGT GTGTGGTAGA TCCACAGATC AAGGATATCT
  481 TGTCTTCGTT GGGAGTGAAT TAGCCCTTCC AGTCCCCCCT TTTCTTTTAA AAAGTGGCTA
  541 AGATCTACAG CTGCCTTGTA AGTCATTGGT CTTAAAGGTA CCTGAGGTGT GACTGGAAAA
  601 CCCACCTCCT CCTCCTCTTG TGCTTCTAGC CAGGCACAAT CAGCATTGGT AGCTGCTGTA
  661 TTGCTACTTG TGATTGCTCC ATGTTTTTCT AGGTCTCGAC GGTATCGATG CGGGGAGGCG
  721 GCCCAAAGGG AGATCCGACT CGTCTGAGGG CGAAGGCGAA GACGCGGAAG AGGCCGCAGA
  781 GCCGGCAGCA GGCCGCGGGA AGGAAGGTCC GCTGGATTGA GGGCCGAAGG GACGTAGCAG
  841 AAGGACGTCC CGCGCAGAAT CCAGGTGGCA ACACAGGCGA GCAGCCATGG AAAGGACGTC
  901 AGCTTCCCCG ACAACACCAC GGAATTGTCA GTGCCCAACA GCCGAGCCCC TGTCCAGCAG
  961 CGGGCAAGCA GCGGCGATGA GTTTCCGCCG TGGCAATAGG GAGGGGGAAA GCGAAAGTCC
 1021 CGAAAGGAGC TGACAGTTGT TGCATGCCCT CACCAGTGGG GGATGCGTCA GCCAACACAG
1081 TGGCAACTCA GCAACGTGCT GAACAACCGG ATCTACAAAC TTCCCTCATT AAA
//
Primer: env_F
Region: cPPT/CTS,CAG pro.5'
Sequence file: RDB15876_A8Cfd.seq check
>05963_15876_3_A8Cf_env_F_C01_07_ABI24.ab1
    1 GAAGGGGAAG GGATCCATTC GGATTAGTGA CGGATCTACA AATGGCAGTA TTCATCCACA
   61 ATTTTAAAAG AAAAGGGGGG ATTGGGGGGT ACAGTGCAGG GGAAAGAATA GTAGACATAA
  121 TAGCAACAGA CATACAAACT AAAGAATTAC AAAAACAAAT TACAAAAATT CAAAATTTTC
  181 GGGTTTATTA CAGGGACAGC AGAAATTCAC TTTGAATTGG GCTGCAGGAA TCTAGTTATT
  241 AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA TATGGAGTTC CGCGTTACAT
  301 AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA CCCCCGCCCA TTGACGTCAA
  361 TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT CCATTGACGT CAATGGGTGG
  421 AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT GTATCATATG CCAAGTACGC
  481 CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA TTATGCCCAG TACATGACCT
  541 TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT CATCGCTATT ACCATGGTCG
  601 AGGTGAGCCC CACGTTCTGC TTCACTCTCC CCATCTCCCC CCCCTCCCCA CCCCCAATTT
661 TGTATTTATT TATTTTTTAA TTATTTTGTG CAGCGATGGG GGCGGGGGGG GGGGGG
//
Primer: RRE-M_R
Region: RRE,HIV-1 psi
Sequence file: RDB15876_A8Cfe.seq check
>05963_15876_A9HgF_RRE-M_R_C01_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGCT GTTGCGCCTC NNTAGCCCTC NGCAAATTGT TCTGCTGCTG
   61 CACTATACCA GACAATAATT GTCTGGCCTG TACCGTCAGC GTCATTGACG CTGCGCCCAT
  121 AGTGCTTCCT GCTGCTCCCA AGAACCCAAG GAACAAAGCT CCTATTCCCA CTGCTCTTTT
  181 TTCTCTCTGC ACCACTCTTC TCTTTGCCTT GGTGGGTGCT ACTCCTAATG GTTCAATTTT
  241 TACTACTTTA TATTTATATA ATTCACTTCT CCAATTGTCC CTCATATCTC CTCCTCCAGG
  301 TCTGAAGATC AGCGGCCGCT TGCTGTGCGG TGGTCTTACT TTTGTTTTGC TCTTCCTCTA
  361 TCTTGTCTAA AGCTTCCTTG GTGTCTTTTA TCTCTATCCT TTGATGCACA CAATAGAGGG
  421 TTGCTACTGT ATTATATAAT GATCTAAGTT CTTCTGATCC TGTCTGAAGG GATGGTTGTA
  481 GCTGTCCCAG TATTTGTCTA CAGCCTTCTG ATGTTTCTAA CAGGCCAGGA TTAACTGCGA
  541 ATCGTTCTAG CTCCCTGCTT GCCCATACTA TATGTTTTAA TTTATATTTT TTCTTTCCCC
  601 CTGGCCTTAA CCGAATTTTT TCCCATCGCG ATCTAATTCT CCCCCGCTTA ATACTGACGC
  661 TCTCGCACCC ATCTCTCTCC TTCTAGCCTC CGCTAGTCAA AATTTTTGGC GTACTCACCA
  721 GTCGCCGCCC CTCGCCTCTT GCCGTGCGCG CTTCAGCAAG CCGANTCCTG CGTCGAGAGA
  781 GCTCCTCTGG TTTCCCTTTC GCTTTCAAGT CCCTGTTCGG GCGCCACTGC TAGAGATTTT
  841 CCACACNNNC TAAAANGGTC TGANGGANCT CTAGTNACCA GANNCACACA ACANANNGGC
  901 ACACACTACT TNAAGCANTC NAGGNNANCT TTATTGNNGN NTNNNCANNN GGGNNCCNNN
961 NNTNAGCNNN NNNNNNN
//
Primer: SV40pro_F
Region: SV40 pro/ori,Zeo,SV40 p(A)
Sequence file: RDB15876_A8Cff.seq check
>05963_15876_A8Cf_3_SV40pro_F_G01_07_ABI08.ab1
    1 ATTGGTCCCG GTAACAAATC AAGGAAGTAG TGAGGAGCTT TTTTGGAGGC CTAGGCTTTT
   61 GCAAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAGCA CGTGTTGACA
  121 ATTAATCATC GGCATAGTAT ATCGGCATAG TATAATACGA CAAGGTGAGG AACTAAACCA
  181 TGGCCAAGTT GACCAGTGCC GTTCCGGTGC TCACCGCGCG CGACGTCGCC GGAGCGGTCG
  241 AGTTCTGGAC CGACCGGCTC GGGTTCTCCC GGGACTTCGT GGAGGACGAC TTCGCCGGTG
  301 TGGTCCGGGA CGACGTGACC CTGTTCATCA GCGCGGTCCA GGACCAGGTG GTGCCGGACA
  361 ACACCCTGGC CTGGGTGTGG GTGCGCGGCC TGGACGAGCT GTACGCCGAG TGGTCGGAGG
  421 TCGTGTCCAC GAACTTCCGG GACGCCTCCG GGCCGGCCAT GACCGAGATC GGCGAGCAGC
  481 CGTGGGGGCG GGAGTTCGCC CTGCGCGACC CGGCCGGCAA CTGCGTGCAC TTCGTGGCCG
  541 AGGAGCAGGA CTGACACGTG CTACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT
  601 TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA
  661 TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA
  721 GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT
  781 TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC GTCGACCTCT AGCTAGAGCT
  841 TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC
  901 ACAACATACG AGCCGGAAGG CATAAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA
  961 ACTCACATTA AATTGCGTTT GCGCTCACTG CCCGGCTTTT CCAGTTCGGA AACCCTGGTC
 1021 GTGGCCAGCT GCCATTAAAT GAATCGGGCC AACGCGCGGG GGAGAGGCGG TTTGCGTATG
 1081 GGGCGGCTCC TCGGCACGTC GCTTCACCTG GACCTCGCCT TGGCGGCCCT TCCGGGTTCC
1141 GGTTCTCG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

user report Frazier, A.E., Fatal Perinatal Mitochondrial Cardiac Failure Caused by Recurrent De Novo Duplications in the ATAD3 Locus. Med (2020)
user report Sampilvanjil, A., Cigarette smoke extract induces ferroptosis in vascular smooth muscle cells. Am. J. Physiol. Heart Circ. Physiol. 318 (3): H508-H518 (2020). PMID 31975626.
user report Borna, N.N., Mitochondrial ribosomal protein PTCD3 mutations cause oxidative phosphorylation defects with Leigh syndrome. Neurogenetics 20 (1): 9-25 (2019). PMID 30607703.
user report Mitani, A., Characterization of doxycycline-dependent inducible Simian Virus 40 large T antigen immortalized human conjunctival epithelial cell line. PLoS One 14 (9): e0222454 (2019). PMID 31509592.
user report Takahashi, S., Loss of p57 KIP2 expression confers resistance to contact inhibition in human androgenetic trophoblast stem cells. Proc. Natl. Acad. Sci. U.S.A. pii: 201916019 (2019). PMID 31792181.
user report Karasawa, T., Saturated Fatty Acids Undergo Intracellular Crystallization and Activate the NLRP3 Inflammasome in Macrophages Arterioscler. Thromb. Vasc. Biol. 38 (4): 744-756 (2018). PMID 29437575.
user report Kawashima, A., ARIH2 Ubiquitinates NLRP3 and Negatively Regulates NLRP3 Inflammasome Activation in Macrophages. J. Immunol. 199 (10): 3614-3622 (2017). PMID 29021376.
user report Kohda, M., A Comprehensive Genomic Analysis Reveals the Genetic Landscape of Mitochondrial Respiratory Chain Complex Deficiencies. PLoS Genet. 12 (1): e1005679 (2016). PMID 26741492.
user report Kishita, Y., Intra-mitochondrial Methylation Deficiency Due to Mutations in SLC25A26. Am. J. Hum. Genet. 97 (5): 761-768 (2015). PMID 26522469.
user report Okada, K., Enhanced flexibility of place discrimination learning by targeting striatal cholinergic interneurons. Nat. Commun., 5: 3778 (2014). PMID 24797209.
user report Nanba, D., Actin filament dynamics impacts keratinocyte stem cell maintenance. EMBO Mol Med., 5 (4): 640-653 (2013). PMID 23554171.
user report Matsushita, N., Doxycycline-dependent inducible and reversible RNA interference mediated by a single lentivirus vector. Biosci. Biotechnol. Biochem., 77 (4): 776-781 (2013). PMID 23563548.
user report Tang, H., CD134 is a cellular receptor specific for human herpesvirus-6B entry. Proc. Natl. Acad. Sci. U.S.A., 110 (22): 9096-9099 (2013). PMID 23674671.
user report Yaguchi, M., Characterization of the properties of seven promoters in the motor cortex of rats and monkeys after lentiviral vector-mediated gene transfer. Hum. Gene Ther. Methods, 24 (6): 333-344 (2013). PMID 23964981.
user report Tamura, K., A glass-coated tungsten microelectrode enclosing optical fibers for optogenetic exploration in primate deep brain structures. J. Neurosci. Methods, 211 (1): 49-57 (2012). PMID 22971353.
user report Fujita, K., Effects of a brain-engraftable microglial cell line expressing anti-prion scFv antibodies on survival times of mice infected with scrapie prions. Cell. Mol. Neurobiol. 31 (7): 999-1008 (2011). PMID 21516351.
user report Tsubota, T., Optogenetic manipulation of cerebellar purkinje cell activity in vivo. PLoS One 6 (8): e22400 (2011). PMID 21850224.
user report Nishie T., Beta4-galactosyltransferase-5 is a lactosylceramide synthase essential for mouse extra-embryonic development. Glycobiology., 20, 1311-1322 (2010). PMID 20574042.
user report Ohashi Y., A bicistronic lentiviral vector-based method for differential transsynaptic tracing of neural circuits. Mol. Cell. Neurosci. 46 (1): 136-147. PMID 20816792.

2020.12.24

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