Expressing FLAG-tagged fusion protein, CMV promoter.
Drawn by SnapGene® software |
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Clone info. | Expression vector. Expressing a fusion protein tagged with single FLAG epitope nuder the control of CMV promoter. |
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Vector backbone | pRc/CMV (Plasmid) |
Size of vector backbone | 5.5 kb |
Selectable markers | Amp^r (E. coli), Neo^r (mammalian cell) |
Growth conditions | 37C, LB+Amp |
Depositor|Developer | DNA Bank, | Murata, Takehide | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL in any publication. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 発表に際し、理研BRCから入手したことを明記すること。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB05956 | pCMV_S-FLAG | DNA solution |
Materials & Methods section:
The pCMV_S-FLAG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05956). |
Reference section:
Further references such as user reports and related articles (go to bottom)
Original, user report and related articles
user_report | Ku, C.C., Jdp2-deficient Granule Cell Progenitors in the Cerebellum Are Resistant to ROS-mediated Apoptosis Through xCT/Slc7a11 Activation. Sci. Rep. 10 (1): 4933 (2020). PMID 32188872. [link to RRC of NBRP] |
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user_report | Kuki, K., Enhancement of TGF-beta-induced Smad3 activity by c-Abl-mediated tyrosine phosphorylation of its coactivator SKI-interacting protein (SKIP). Biochem. Biophys. Res. Commun. 490 (3): 1045-1051 (2017). PMID 28666867. [link to RRC of NBRP] |
user_report | Hayashi, S., Lin28a is a putative factor in regulating cancer stem cell-like properties in side population cells of oral squamous cell carcinoma. Exp Cell Res. 2013 May 1;319(8):1220-8. PMID 23500413. [link to RRC of NBRP] |
user_report | Shimizu, H., NF-kappaB plays an important role in indoxyl sulfate-induced cellular senescence, fibrotic gene expression, and inhibition of proliferation in proximal tubular cells. Am. J. Physiol. Cell Physiol. 301 (5): C1201-12 (2011). PMID 21832251. [link to RRC of NBRP] |
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