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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCMV_S-FLAG

Expressing FLAG-tagged fusion protein, CMV promoter.

Catalog number RDB05956
Resource name pCMV_S-FLAG
Clone info. Expression vector. Expressing a fusion protein tagged with single FLAG epitope nuder the control of CMV promoter.
Vector backbone pRc/CMV (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Depositor|Developer DNA Bank, | Murata, Takehide |
Other clones in our bank
External Database
            Reference sequence
              
             
            Sequence (full) RDB05956zzt03.seq compiled by us
            Remarks, protocol and/or map (gif) RDB05956a.gif

            Distribution information

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            RDB05956 pCMV_S-FLAG DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pCMV_S-FLAG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05956).

            Reference section:

            Further references such as user reports and related articles (go to bottom)

            References and tips

            Featured content

            Featured content Dnaconda's recommendation EXR0039e (English text)
            Featured content Dnaconda's recommendation EXR0039j (Japanese text)
            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)
            Featured content pCMV_tag and pRSV_tag vector (English text)
            Featured content pCMV_tag and pRSV_tag vector (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB17720_A9K8p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CMV_Forward (Pr0016)
            Region: CMV pro,FLAG,bGHp(A),f1 ori
            Sequence file: RDB17720_A9K8a.seq check
            >05956_17220_A9K8_CMV-Forward_A08_01_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNTN NNNNCNNNGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
               61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
              121 CGACTCACTA TAGGGAGACC CAAGCTGAAT TCACCATGGA CTACAAGGAC GATGACGATA
              181 AGGCGGCCGC TCGAGCATGC ATCTAGAGGG CCCTATTCTA TAGTGTCACC TAAATGCTAG
              241 AGCTCGCTGA TCAGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG TTTGCCCCTC
              301 CCCCGTGCCT TCCTTGACCC TGGAAGGTGC CACTCCCACT GTCCTTTCCT AATAAAATGA
              361 GGAAATTGCA TCGCATTGTC TGAGTAGGTG TCATTCTATT CTGGGGGGTG GGGTGGGGCA
              421 GGACAGCAAG GGGGAGGATT GGGAAGACAA TAGCAGGCAT GCTGGGGATG CGGTGGGCTC
              481 TATGGCTTCT GAGGCGGAAA GAACCAGCTG GGGCTCGAGG GGGGATCCCC ACGCGCCCTG
              541 NNNNNGCGCA TTAANNNNNN CGGGTGTGGT GGTTACNCNC ANNNNNNCCG CTACACTTGC
              601 CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG
              661 CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA NTGCTTTACG
              721 GCNNCCTCGA CCCCAAAAAA NNTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC
            781 TGATAGACGN NTTTTCNCCC TTTTGACGTT GGNNNNNNCG TNCNNNNNNN NNN
            //
            Primer: SV40pro_ori_F (Pr0731)
            Region: SV40pro_ori,NeoR
            Sequence file: RDB17720_A9K8b.seq check
            >05956_17220_A9K8_SV40pro_ori_F_B08_02_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNG CCTCGNCCTC TGAGCTATTC CNGAAGTAGT GAGGAGGCTT
               61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGGATATCCA TTTTCGGATC
              121 TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG
              181 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG
              241 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA
              301 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT
              361 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA
              421 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC
              481 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC
              541 CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC
              601 CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT
              661 GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA
              721 TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACNGTGG
              781 CCGGCTGGGT GTGGCGGANC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA
              841 AGAGCTNGNC GGCGAANGGG CTGACCGCTN NCTCGTGCTT TACNGTATCG CCNCNNCNNA
              901 TTCNCANCGC ANCGCCNTCT ATCGCCNTCT TGANNANTTC TTCTGANCGG GNNTNTNGGG
            961 TTCNNNNNNA NCNNNNNNNC NANNCCNNNN CNNGNCNTCN CNANNTTTCN N
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            user_report Ku, C.C., Jdp2-deficient Granule Cell Progenitors in the Cerebellum Are Resistant to ROS-mediated Apoptosis Through xCT/Slc7a11 Activation. Sci. Rep. 10 (1): 4933 (2020). PMID 32188872.
            user_report Kuki, K., Enhancement of TGF-beta-induced Smad3 activity by c-Abl-mediated tyrosine phosphorylation of its coactivator SKI-interacting protein (SKIP). Biochem. Biophys. Res. Commun. 490 (3): 1045-1051 (2017). PMID 28666867.
            user_report Hayashi, S., Lin28a is a putative factor in regulating cancer stem cell-like properties in side population cells of oral squamous cell carcinoma. Exp Cell Res. 2013 May 1;319(8):1220-8. PMID 23500413.
            user_report Shimizu, H., NF-kappaB plays an important role in indoxyl sulfate-induced cellular senescence, fibrotic gene expression, and inhibition of proliferation in proximal tubular cells. Am. J. Physiol. Cell Physiol. 301 (5): C1201-12 (2011). PMID 21832251.

            2023.05.22

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