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pCMV_S-FLAG

Expressing FLAG-tagged fusion protein, CMV promoter.

Catalog number RDB05956
Resource name pCMV_S-FLAG
Clone info. Expression vector. Expressing a fusion protein tagged with single FLAG epitope nuder the control of CMV promoter.
Vector backbone pRc/CMV (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Depositor|Developer DNA Bank, | Murata, Takehide |
 
Sequence (full) RDB05956zzt03.seq compiled by us
Remarks, protocol and/or map (gif) RDB05956a.gif

Distribution information

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RDB05956 pCMV_S-FLAG DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMV_S-FLAG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05956).

Reference section:

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17720_A9K8p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,FLAG,bGHp(A),f1 ori
Sequence file: RDB17720_A9K8a.seq check
>05956_17220_A9K8_CMV-Forward_A08_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNTN NNNNCNNNGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
  121 CGACTCACTA TAGGGAGACC CAAGCTGAAT TCACCATGGA CTACAAGGAC GATGACGATA
  181 AGGCGGCCGC TCGAGCATGC ATCTAGAGGG CCCTATTCTA TAGTGTCACC TAAATGCTAG
  241 AGCTCGCTGA TCAGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG TTTGCCCCTC
  301 CCCCGTGCCT TCCTTGACCC TGGAAGGTGC CACTCCCACT GTCCTTTCCT AATAAAATGA
  361 GGAAATTGCA TCGCATTGTC TGAGTAGGTG TCATTCTATT CTGGGGGGTG GGGTGGGGCA
  421 GGACAGCAAG GGGGAGGATT GGGAAGACAA TAGCAGGCAT GCTGGGGATG CGGTGGGCTC
  481 TATGGCTTCT GAGGCGGAAA GAACCAGCTG GGGCTCGAGG GGGGATCCCC ACGCGCCCTG
  541 NNNNNGCGCA TTAANNNNNN CGGGTGTGGT GGTTACNCNC ANNNNNNCCG CTACACTTGC
  601 CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG
  661 CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA NTGCTTTACG
  721 GCNNCCTCGA CCCCAAAAAA NNTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC
781 TGATAGACGN NTTTTCNCCC TTTTGACGTT GGNNNNNNCG TNCNNNNNNN NNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40pro_ori,NeoR
Sequence file: RDB17720_A9K8b.seq check
>05956_17220_A9K8_SV40pro_ori_F_B08_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNG CCTCGNCCTC TGAGCTATTC CNGAAGTAGT GAGGAGGCTT
   61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGGATATCCA TTTTCGGATC
  121 TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG
  181 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG
  241 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA
  301 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT
  361 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA
  421 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC
  481 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC
  541 CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC
  601 CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT
  661 GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA
  721 TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACNGTGG
  781 CCGGCTGGGT GTGGCGGANC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA
  841 AGAGCTNGNC GGCGAANGGG CTGACCGCTN NCTCGTGCTT TACNGTATCG CCNCNNCNNA
  901 TTCNCANCGC ANCGCCNTCT ATCGCCNTCT TGANNANTTC TTCTGANCGG GNNTNTNGGG
961 TTCNNNNNNA NCNNNNNNNC NANNCCNNNN CNNGNCNTCN CNANNTTTCN N
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

user_report Ku, C.C., Jdp2-deficient Granule Cell Progenitors in the Cerebellum Are Resistant to ROS-mediated Apoptosis Through xCT/Slc7a11 Activation. Sci. Rep. 10 (1): 4933 (2020). PMID 32188872. [link to RRC of NBRP]
user_report Kuki, K., Enhancement of TGF-beta-induced Smad3 activity by c-Abl-mediated tyrosine phosphorylation of its coactivator SKI-interacting protein (SKIP). Biochem. Biophys. Res. Commun. 490 (3): 1045-1051 (2017). PMID 28666867. [link to RRC of NBRP]
user_report Hayashi, S., Lin28a is a putative factor in regulating cancer stem cell-like properties in side population cells of oral squamous cell carcinoma. Exp Cell Res. 2013 May 1;319(8):1220-8. PMID 23500413. [link to RRC of NBRP]
user_report Shimizu, H., NF-kappaB plays an important role in indoxyl sulfate-induced cellular senescence, fibrotic gene expression, and inhibition of proliferation in proximal tubular cells. Am. J. Physiol. Cell Physiol. 301 (5): C1201-12 (2011). PMID 21832251. [link to RRC of NBRP]