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pKM2L-phPSA(3) (#RDB05910)

Promoter Bank clone, Human prostate-specific antigen/kallikrein 3 (PSA/KLK3) promoter


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Remarks, protocol and/or map (pdf) RDB05910.pdf
Publication Unpublished bioresource
Test sheet Data Sheet open 
 
Alternative name p1996-8F
Clone info. PCR amplified human prostate-specific antigen/kallikrein 3 (PSA/KLK3) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 50854208 to 50854952(NC_000019.10); relatively -707 to +38, where +1 corresponds to 1 nt of NM_001648.2.
Comment PCR cloning, forward primer, 1996 F2: 5' agggcaggtcagcaggcatctctgt 3'; reverse primer, 1996 R2: 5' gacacagctctccgggtgcaggtgg 3' (reference: J. Biol. Chem., 271, 6379-6388, 1996, PMID:8626436)
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Growth conditions 37C, LB+Kan
Gene/insert name Human prostate-specific antigen/kallikrein 3 (PSA/KLK3) genomic DNA
Depositor|Developer DNA Bank, |

Distribution information

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RDB05910 pKM2L-phPSA(3) DNA solution

How to cite this biological resource

Materials & Methods section:

The pKM2L-phPSA(3) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05910).

Reference section:

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles