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pKM2L-LHB

Promoter Bank clone, Human luteinizing hormone-beta (LHB) promoter

Catalog number RDB05906
Resource name pKM2L-LHB
Alternative name p1992-16F
Clone info. PCR amplified human luteinizing hormone-beta (LHB) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 49018394 to 49017083(NC_000019.10); relatively -1304 to +8, where +1 corresponds to 1 nt of NM_000894.2.
Comment Reference sequence, NG_000019. PCR cloning, forward primer, 1992 F2: 5' aactcgtccccaaggatccagctgt 3'; reverse primer, 1992 R2: 5' cttggtgcatcccctgcctcgtgtatc 3' (reference: J. Biol. Chem., 278, 24033-24038, 2003, PMID:12700240; Mol. Cell. Biol., 19, 2567-2576, 1999, PMID:10082522; Mol. Endocrinol., 13, 752-763, 1999, PMID:10319325) Entire sequence of promoter region has not been confirmed. Promoter activity has not been analyzed.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human luteinizing hormone-beta (LHB) genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human luteinizing hormone-beta (LHB)

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          RDB05906 pKM2L-LHB Under QC test. Please contact us. DNA solution

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          References and tips

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          Original reference

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          Sequence information

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          References

          Original, user report and related articles


          2022.05.18

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