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pKM2L-phGH1(2) (#RDB05905)

Promoter Bank clone, Human growth hormone (GH1)>CS-1 (9/6/05) promoter

Alternative name p1991-33F
Clone info. PCR amplified human growth hormone (GH1)>CS-1 (9/6/05) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 63919578 to 63918807(NC_000017.11); relatively -726 to +46, where +1 corresponds to 1 nt of NM_000515.4.
Comment PCR cloning, forward primer, 1991 F2: 5' gggaggaagagcttgtggtccgtgt 3'; reverse primer, 1991 R2: 5' gagtggttcggggagttgggcctt 3' (reference: DNA, 5, 503-509, 1986, PMID:3469066; EMBO J., 6, 971-981, 1987, PMID:3595566) Entire sequence of promoter region has not been confirmed.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human growth hormone 1 genomic DNA
Depositor|Developer DNA Bank, |
 

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Terms and conditions for distribution 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB05905 pKM2L-phGH1(2) Under QC test. Please contact us. DNA solution

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How to cite this biological resource

Materials & Methods section:

The pKM2L-phGH1(2) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05905).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

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References

Original, user report and related articles