Promoter Bank clone, Human ventricular myosin light chain 2 (MLC-2V) promoter
Alternative name | p1986-23F |
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Clone info. | PCR amplified human ventricular myosin light chain 2 (MLC-2V) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 110922900 to 110920544(NC_000012.12); relatively -2300 to +57, where +1 corresponds to 1 nt of NM_000432.3. |
Comment | PCR cloning, forward primer, 1986 F2: 5' cactgccatgcctctgtctcatccc 3'; reverse primer, 1986 R2: 5' agcactgcctcccgagaagaattcc 3' (reference: Genomics, 13, 829-831, 1992, PMID:1386340; Biochem. Biophys. Res. Commun., 210, 260-266, 1995, PMID:7755599; Mol. Cell. Biol., 14, 7331-7339, 1994, PMID:7935447) Entire sequence of promoter region has not been confirmed. |
Vector backbone | pKM2L (RDB04026) (Plasmid) |
Size of vector backbone | 4.2 kb |
Selectable markers | Kan^r |
Gene/insert name | Human myosin, light chain 2, regulatory, cardiac, slow genomic DNA |
Depositor|Developer | DNA Bank, | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation. |
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Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB05900 | pKM2L-phMLC2V | Under QC test. Please contact us. | DNA solution |
Materials & Methods section:
The pKM2L-phMLC2V was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05900). |
Reference section:
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
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