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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pKM2L-phVEC

Promoter Bank clone, Human vascular endothelial (VE)-cadherin/cadherin-5 promoter

Catalog number RDB05899
Resource name pKM2L-phVEC
Alternative name p1985-27F
Clone info. PCR amplified human vascular endothelial (VE)-cadherin/cadherin-5 promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 66363438 to 66366746(NC_000016.10); relatively -3203 to +106, where +1 corresponds to 1 nt of NM_001795.5.
Comment PCR cloning, forward primer, 1985 F2: 5' gtgtcccgactcaattacagca 3'; reverse primer, 1985 R2: 5' ctgagggatgtttctgttccgtt 3' (reference: Oncogene, 24, 2992-3001, 2005, PMID:15735710) Entire sequence of promoter region has not been confirmed. Promoter activity has not been analyzed.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human cadherin 5, type 2, VE-cadherin (vascular epithelium) genomic DNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Human cadherin 5, type 2, VE-cadherin (vascular epithelium)
          Reference sequence
            AC012325

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          RDB05899 pKM2L-phVEC DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12752_A4D9.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40
          Sequence file: RDB12752_A4D9a.seq check
          1
          Primer: phRLR2
          Sequence file: RDB12752_A4D9b.seq check
          1

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl