RDB05899 - pKM2L-phVEC

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pKM2L-phVEC (#RDB05899)

Promoter Bank clone, Human vascular endothelial (VE)-cadherin/cadherin-5 promoter

Alternative name	p1985-27F
Clone info.	PCR amplified human vascular endothelial (VE)-cadherin/cadherin-5 promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 66363438 to 66366746(NC_000016.10); relatively -3203 to +106, where +1 corresponds to 1 nt of NM_001795.5.
Comment	PCR cloning, forward primer, 1985 F2: 5' gtgtcccgactcaattacagca 3'; reverse primer, 1985 R2: 5' ctgagggatgtttctgttccgtt 3' (reference: Oncogene, 24, 2992-3001, 2005, PMID:15735710) Entire sequence of promoter region has not been confirmed. Promoter activity has not been analyzed.
Vector backbone	pKM2L (RDB04026) (Plasmid)
Size of vector backbone	4.2 kb
Selectable markers	Kan^r
Gene/insert name	Human cadherin 5, type 2, VE-cadherin (vascular epithelium) genomic DNA
Depositor\|Developer	DNA Bank, \|

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Terms and conditions for distribution	1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB05899	pKM2L-phVEC	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

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How to cite this biological resource

Materials & Methods section:

The pKM2L-phVEC was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05899).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.