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pKM2L-phESEL (#RDB05894)

Promoter Bank clone, Human E-selectin/Endothelial leukocyte adhesion molecule 1 (ELAM-1)/leukocyte-endothelial cell adhesion molecule 2 (LECAM2)/CD62E antigen promoter

Alternative name p1979-4F
Clone info. PCR amplified human E-selectin/Endothelial leukocyte adhesion molecule 1 (ELAM-1)/leukocyte-endothelial cell adhesion molecule 2 (LECAM2)/CD62E antigen promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 169735760 to 169733973(NC_000001.11); relatively -1681 to +107, where +1 corresponds to 1 nt of NM_003006.4.
Comment PCR cloning, forward primer, 1979 F2: 5' agatagggtaagatgcagagatgggg 3'; reverse primer, 1979 R2: 5' ttttgggaagcagttgttcaaacac 3'; Entire sequence of promoter region has not been confirmed. Promoter activity has not been analyzed. (reference: Nucleic Acids Res., 19, 2645-2653, 1991, PMID:1710341; Proc. Natl. Acad. Sci. U S A, 88, 6523-6527, 1991, PMID:1713680)
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human E-selectin/Endothelial leukocyte adhesion molecule 1 (ELAM-1)/leukocyte-endothelial cell adhesion molecule 2 (LECAM2)/CD62E antigen genomic DNA
Depositor|Developer DNA Bank, |
 

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Terms and conditions for distribution 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
提供案内 (日本国内) [open/close]

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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB05894 pKM2L-phESEL Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

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How to cite this biological resource

Materials & Methods section:

The pKM2L-phESEL was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05894).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

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References

Original, user report and related articles