RDB05891 - pKM2L-phBGR2

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pKM2L-phBGR2 (#RDB05891)

Promoter Bank clone, Human brain glutamate receptor 2 (HBGR2) promoter

Alternative name	p1975-58F
Clone info.	PCR amplified human brain glutamate receptor 2 (HBGR2) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 157219521 to 157221033(NC_000004.12); relatively -1207 to +306, where +1 corresponds to 1 nt of NM_000826.4.
Comment	PCR cloning, forward primer, 1975 F2: 5' acataaatttcacacgggaagtcca 3'; reverse primer, 1975 R2: 5' aagtagagcatccacaatatacccc 3'; Entire sequence of promoter region has not been confirmed. Promoter activity has not been analyzed. (reference: J. Neurosci., 18, 6723-6739, 1998, PMID:9712644)
Vector backbone	pKM2L (RDB04026) (Plasmid)
Size of vector backbone	4.2 kb
Selectable markers	Kan^r
Gene/insert name	Human glutamate receptor, ionotropic, AMPA 2 genomic DNA
Depositor\|Developer	DNA Bank, \|

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Terms and conditions for distribution 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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MTAに書く使用条件	1. 本件リソースは学術研究機関にのみ提供し、学術研究にのみ利用することができる。　2. 謝辞の表明を必要とする。 MTAに書く付加的使用条件：本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB05891	pKM2L-phBGR2	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pKM2L-phBGR2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05891).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB05891_A4Lm.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 Sequence file: RDB05891_A4Lma.seq
>05891_05891_A4Lm_3_M13(-40)_E08_14.ab1 1 CCGGCGTCAG CTTGCATGCC TGCAGGTCAT TTAATAGGGA TAACAGCGGT AATGATTAAG 61 ATCTCGAGCT CTAAGCTTCA CATATGCATG CACTAGTGGC ACATAAATTT CACACGGGAA 121 GTCCAGTCAT CCATCTCGCC ATCAATTCTC TCCCTTCAAA CTTTTAATAT CTAACCTCAT 181 CTTTCTTTCC CTGTGGCCCG CGTGTTCAGT AGAGCGTGGT GGCTTACTTT CTTTAGGCAG 241 TGATTTTTCG AAACCCCCGG CCAAAACGCG ATGGGGAAAC AGATTTCTAA GTAAGTCTCT 301 GGGAAAGAAT CTGCATCTCT GTGGCATTCC TACACTAGCT TCATCTTCTA ATCACTTTCC 361 TCTAATAGCC TTTCCTTAAA CCAGAGTCTC TTGCTTTCAA TCTGCATAAA GCAAGGGCTT 421 CTAGGAAACC AAGTCTACAT GACTCTACAA CGGGAGGGAT TGCAGTGAAA GCTGCGAGAA 481 AGACCCGTGC GCTCTGTAAC TAGGCGAGTC AGCATCACAG CCCAATGGAA TATACTCTAA 541 AGATGGGATT TTCATGCAAA ATATTTAAAA GATTCCATCT TGATCTCTTG TGCGGCTTAA 601 GCATTTAAAG ATATAACTTG GGGTTCTCCC TGGATCCTGG CTACATCCCA TGAAGCCCAA 661 GTGCACAATT TTGGAATATT TCCTCCACGG TGCTTTGCTT GCTTGTTTGC TTTCGGCTGG 721 GTCAGGTGAG GAGGATGACA GGGCTTCTGT ATTGCTAAAT GGGTTTTCAC AGGAGATGGA 781 AGATAAAAAT CAAAATTATT TGTAACGTAA GACAGCAGGG CCTGGTGAGA GGACGCTTCG 841 CCGCCAACAA TTAGCAATTC GGCTTCTACA CAGCAGCCGG AGATCAGCTT TGCTGCATTT 901 GGTCCAGGTT GGAGCATCTC CGCAGCAGCT GCAACAGCCG CACGAAAGGT AGCTCCGGGC 961 GGGGAGCGAG GCGCTGTCCT CGGTGCTGAA AGGCCGAGGC GCGCGGTGGG CGCGACAGCC 1021 CCGGAGACCC GAGGTCTCGC GGAGGGACAG CGGCTACGGG CCCCGAGCTG TGCTTTCTCA 1081 GCGCCGCGCA CGCGACGCGT CACGTTGATG CGGGATGCCG GGCGTCGTGC CGCGGGGGGA 1141 AAGAGGGGGG GTCTCCTCGA CT //
Primer: phRLR2 Sequence file: RDB05891_A4Lmb.seq
>05891_05891_A4Lm_3_phRLR2_F08_17.ab1 1 AACTCCGTCT GAAGCCCAGT TTCTATTGGT CTCCTTAAAC CTGTCTTGTA ACCTTGATAC 61 TTACCTGCCC AGTGCCTCAC GACCAACTTC TGCAGTACCC GGGTGAATTC TACGCGTCGA 121 CAGGCAAGTA GAGCATCCAC AATATACCCC TTTTTTTTTC CTTTTCCTCC TCTTCCTTCT 181 TTGGCTGTTT TCCTCTGCAA ATTAGATCCT CTGCATTTTG AGACAGCAAT TTAGCACCAA 241 GCTGCTAAAA CCTGAAGTGG AGGCAGAAGA ATCCCTATTT CCCAGGTCCT GCTGGTGGCT 301 GCTGATGCCC TGCGCCCTGT CCCCGCTCCA GAAGTCCGGA GGACTGGCTG AATGCAGTTT 361 TCAGCGGAGC TGCCGCTGTG GTCCCAGTGT CTCGGAATAT TCAGCACCCT CCCATGCACT 421 CACACACACT CACCCTCTCT CGCGCTCTCT TCTCCCTCTC TCCTCTCTCA CTCACGCGCG 481 CGCGCACACA CACACACACA CATACACACA CGCACACACA CAGGCAAGTC ACAGAGAGGG 541 GCAGGCAGTC CCGGGCGCGC GTGCGCGACT CGAGGCAGGA GGCGGGAGCG CGCGCCCCCT 601 CCCCCGCACG GCGCCCGGCA CCCCGCACCA CCGTGGACGC GTCGCGTGCG CGGCGCTGAG 661 AAAGCACAGC TCGGGGCCCG TAGCCGCTGT CCCTCCGCGA GACCTCGGGT CTCCGGGGCT 721 GTCGCGCCCA CCGCGCGCCT CGGCCTTTCA GCACCGAGGA CAGCGCCTCG CTCCCCGCCC 781 GGAGCTACCT TCGTGCGGCT GTTGCAGCTG CTGCGGAGAT GCTCCAACCT GGACCAAATG 841 CAGCAAAGCT GATCTCCGGC TGCTGTGTAG AAGCCGAATT GCTAATTGTT GGCGGCGAAG 901 CGTCCTCTCA CCAGGCCCTG CTGTCTTACG TTACAAATAA TTTTGATTTT TATCTTCCAT 961 CTCCTGTGAA AACCCATTTA GCAATACAGA AGCCCTGTCA TCCCTCCTCA CCTGACCCAG 1021 CCCGAAAGCA AACAGCAAGC AAAGCACCGT GGAAGGAAAT ATTCCAAAAT TGTGCACTTT 1081 GGGCTTTCAT GGGAATGTAG CAAGGAATCC AGGAGACCCA AGCTATATTC TTTGAATGCT 1141 TAGCTCGCCA CAGAGAATTC AAGAGAAGTG GGGGA //