Please review the QC test results indicated by icon below as well as clone information before placing your order.

pKM2L-phSMAD7 (#RDB05888)

Promoter Bank clone, Human Smad7 promoter

Alternative name	p1953-8F
Clone info.	PCR amplified human Smad7 promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). This clone has genomic sequence (48,951,746 to 48,955,347nt of NC_000018.10) corresponding -3.3 kb to +270 bp (tanscription start site P1 is designated +1. Fig. 2 of Denissova, N.G. et al. Proc. Natl. Acad. Sci. USA 97: 6397-6402, 2000). Note that this clone does not contain any part of cDNA sequence of NM_005904.3, a representative sequence of human SMAD7. Alternatively, 48955347 to 48951747(NC_000018.10); relatively -4636 to -1035, where +1 corresponds to 1 nt of NM_005904.3 was cloned. (reference: Proc. Natl. Acad. Sci. U.S.A., 97, 6397-6402, 2000, PMID:10823886; J. Biol. Chem., 275, 29308-2917, 2000, PMID:10887185)
Comment	PCR cloning, forward primer, 1953 F4: 5' gtgtgctccggcctctcattacag 3'; reverse primer, 1953 R4: 5' tcctcctccgagactctcctcgt 3' Known differences (at least): C insertion between 48,952,117 and 48,952,118nt. G to A substitution corresponding to 48,951,914nt of NC_000018.10. Entire sequence of promoter region has not been confirmed.
Vector backbone	pKM2L (RDB04026) (Plasmid)
Size of vector backbone	4.2 kb
Selectable markers	Kan^r
Gene/insert name	Human Smad7 genomic DNA
Depositor|Developer	DNA Bank, |

 

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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB05888	pKM2L-phSMAD7	DNA solution

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The pKM2L-phSMAD7 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05888).

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB05888_A9Cfp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 () Sequence file: RDB05888_A9Cfa.seq
>05888_05888_A9Cf_M13_-40_E01_05_ABI08.ab1     1 AAGCAGTTCA AGCTTGCATG CCTGCAGGTC ATTTAAATAG GGATAACAGG GTAATGATTA    61 AGATCTCGAG CTCTAAGCTT CACATATGCA TGCACTAGTG GCGTGTGCTC CGGCCTCTCA   121 TTACAGAGCC TTAGCACTAC TGGCCAGATC TCCAAAAGAT TCTTCAGATT CTCCATTTAG   181 CCAAGAAACT GAATTAGAAT TATGACCTCT ACTTTCTCTG CTTATTTAAG TCCTTTCTGT   241 TGCTATGAAG CCTGGTCTGA CCACTCTCAA TAAGTGAAGA TCTTTTAGGG AGATCCAGAA   301 CAGAGAGGGA ATGGGACCCT AGAGAGCGGA AACTTGCCCA GAAAATCTTA TAAACTCTGC   361 CAAGTGCTCG CCTAACGTGG GGATTGCTGT TGGCCCTAGT TGCTTAACCA GTGAGCGGCA   421 ACGCCCCAGC CTGGAATCAG GTTTTCTGAC CTCTAGTCTG GTACTCTTTC TAAGCCTTTC   481 TAAGGTGAAA ATATGATACA TTTCCCAGAC ACCTTAAAAA TCCCATTTCT GGGCCGGGCG   541 CTGTGGCTCA TGCCTGTTAT CCCACACTTT GGGAGGCTGA GGCGGGTGGA TCGCCTGAGC   601 TCAGGAGTTC GAGACTAGCC TGGGCAACAC GGTGAAACCT TGTCTCTGCT AAAATACAAA   661 AAAATTAGCG AGGTGTGGAG TGCACGTGTA GTTCCAGCTA CTAGGGAGGC TGAGGCAGGA   721 GAATTGCTTG AACCCAGGAG GCGGAGGTTG CAGTGAGCTG AGATCATGCC ACTGCCCTCT   781 AGGCTGGGTG ACAAAGCAAG GCTCCATCTC AAAAAAAAAA AAAAAAAAAA TCCCACTTCT   841 GTCTCTTTCG TCTTCCCTTG CCTATCATGA TAGGCCTCCC AAAGTGCTGG GATTACACTC   901 CTTCTCCATT CTCTCCTGTG CTTTCTCAGC TGCTGTCTTC ACAGGTTGCT TCTTAGAATG   961 TTTTTTTTAA TACACACACA AGGTTTCAAT GCCCTTTTAT ACAAGCCATA TTTTAATTTA  1021 CACAAAATTA TGATGTTATA CACTGCAGCT TATGGTGGCC TTTACACAGT AAATGTTCAG  1081 ATTGCAGCTT CTGTCGTCAG TAGGATCTGC ACTGCTGCAT CTACTATCCT TCAAGGTCAT 1141 AAAGGTGTTT AAGGAATTCT TGCT //
Primer: phRLR2primer () Sequence file: RDB05888_A9Cfb.seq
>05888_05888_A9Cf_phRLR2primer_F01_06_ABI08.ab1     1 ATTCTTTGTC TCGAAAGCCC AGTTTCTATT GGTCTCCTTA AACCTGTCTT GTAACCTTGA    61 TACTTACCTG CCCAGTGCCT CACGACCAAC TTCTGCAGTA CCCGGGTGAA TTCTACGCGT   121 CGACAGGCTC CTCCTCCGAG ACTCTCCTCG TCGCGCCCGG GAGCCTCCTT GTCCCCGGTC   181 CGCCCTCTCC TGGCGCTCCG GTCCTTCTGC CGCCGCCAGG GGCTCGCCGC GCCGCACTCA   241 GGGGCTCAGG GGCCGGGCGC TCGGCGGCTC GGCGCCGACG GACTGGCTCT GTCTCAGGCA   301 GCTCTCTCCC GCGCGGCGAG CGGACCGAGC ACGGCGCCCG GCTGGCTCGG CTGGCGCGGC   361 TCGGGGACAG GATCTTCCGT GCGCCGAGCA GCAAGCGAGT GTGCCCGGGG CTCACCGCCT   421 CCCGCAAGGC CTCCCGCCCC CGCCCCCTTC CCTCCCCCTT CCTCCCCCTT CCCTCCCCCG   481 CCCCCAGCCG CCGCAGCCGC CGCCGCCTCC TCCCCGCCCC CCCGCACCCC CCCTCCGGCC   541 CTCTGCTCGG CTGGTTCCAC TGCGCAGTGG CGCGCCCGGC TCCGGCCTCG TCACGTGGCC   601 GTCTAGACAC CCTGTCGCTT TAAAAAAAAA AAAAGCGATT GTGTTTCGCA AACAACAGAT   661 CGGGTTTCTA AAAGCTATTT CTCCCCCCAA CCCCCCGCCA CCGCCACCCC CTCCCGGGTC   721 TGTAGAGGGG GTACCGATGG AGGGGAGAGA GATAGGTGGG GGGCAGAGAA GCTCCCAGAA   781 TGGATTGAGC CCCGGCCGGA GCCATGGAGA AATTGGGAAA AGCAGGAGCA CCGAGGCGGG   841 CTTCGCCGCG AGTTTTGAGC TGAGCGAGCG GGTCGTGGCC CGATTTCGAC CGGCTGGGTT   901 TCCGCGGTGC ATCTCGCGCG GGTCGGCTTA GCGCGCTCAT TCATGGATTA TGTTAAAGCC   961 CTGCGGTGAT CCTTGCCCGC CCCGAGGCAG GGAGGAAGCG CTGATCCGGT AAGCTTACGG 1021 ACTGACTAGG CTGGAAGTGC CTTAGGACAT CG //
Primer: pAxCALNLF1 () Sequence file: RDB05888_A9Cfc.seq
>05888_05888_A9Cf_pAxCALNLF1_G01_07_ABI08.ab1     1 TACAAATGTA TCTTATCATG TCTGCTCGAA GCGGCCGCTC TAGAATTACT GCTCGTTCTT    61 CAGCACGCGC TCCACGAAGC TCTTGATGTA CTTACCCATT TCATCTGGAG CGTCCTCCTG   121 GCTGAAGTGG AGGCCCTTCA CCTTCACGAA CTCGGTGTTA GGGAACTTCT TAGCTCCCTC   181 GACAATAGCG TTGGAAAAGA ACCCAGGGTC GGACTCGATG AACATCTTAG GCAGATCGTC   241 GCTGGCCCGA AGGTAGGCGT TGTAGTTGCG GACAATCTGG ACGACGTCGG GCTTGCCTCC   301 CTTAACGAGA GGGATCTCGC GAGGCCAGGA GAGGGTAGGC CGTCTAACCT CGCCCTTCTC   361 CTTGAATGGC TCCAGGTAGG CAGCGAACTC CTCAGGCTCC AGTTTCCGCA TGATCTTGCT   421 TGGGAGCATG GTCTCGACGA AGAAGTTATT CTCAAGCACC ATTTTCTCGC CCTCTTCGCT   481 CTTGATCAGG GCGATATCCT CCTCGATGTC AGGCCACTCG TCCCAGGACT CGATCACGTC   541 CACGACACTC TCAGCATGGA CGATGGCCTT GATCTTGTCT TGGTGCTCGT AGGAGTAGTG   601 AAAGGCCAGA CAAGCCCCCC AGTCGTGGCC CACAAAGATG ATTTTCTTTG GAAGGTTCAG   661 CAGCTCGAAC CAAGCGGTGA GGTACTTGTA GTGATCCAGG AGGCGATATG AGCCATTCCC   721 GCTCTTGCCG GACTTACCCA TTCCGATCAG ATCAGGGATG ATGCATCTAG CCACGGGCTC   781 GATGTGAGGC ACGACGTGCC TCCACAGGTA GCTGGAGGCA GCGTTACCAT GCAGAAAAAT   841 CACGGCGTTC TCGGCGTGCT TCTCGGAATC ATAGTAGTTG ATGAAGGAGT CCAGCACGTT   901 CATTTGCTTG CAGCGAGCCC ACCACTGAGG CCCAGTGATC ATGCGTTTGC GTTGCTCGGG   961 GGTCGTACAC CCTTGGAAGC CATGGTGGCT AGCCTATAGT GAGTCGTATT AAGTACTCTA  1021 GCCTTTAAGA GCTGTAATTT GAACTGGGAG TGGACACCTG TGGAGAAGAA AGGCAAAGTG  1081 GAATGTCAGT AAGAACCAAT AGGTGCCTAT CAGAACGGCA GAGTCCTTCT CTGTCTCGAT 1141 AGCCCCAGAT TTTCTAGTAT TG //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

Resource search

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Link

Other resources in our bank

• human Smad7

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External Database
Human Smad7

• in KEGG Gene
• in NCBI Gene

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Reference sequence
  AC114684

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Featured content

• Promoter Collection (Renilla Luciferase) (English text)

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