RDB05882 - pKM2L-phHKII

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pKM2L-phHKII (#RDB05882)

Promoter Bank clone, Human hexokinase II promoter

Alternative name	p1899-19F
Clone info.	PCR amplified human hexokinase II promoter sequence (Malkki, M. et al., Diabetologia, 40, 1461-1469, 1997, PMID:9447955.) was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 74,832,751 to 74,834,245 (NC_000002.12); relatively -1375 to +120, where +1 corresponds to 1 nt of XM_005264280.2; relatively +96 to +1591, where +1 corresponds to 1 nt of NM_000189.4.
Comment	PCR cloning, forward primer, 1899 F2: 5' attgcagacagcaccccactgtt 3'; reverse primer, 1899 R2: 5' agtgggacctctccgattcacagg 3' Entire sequence of promoter region has not been confirmed.
Vector backbone	pKM2L (RDB04026) (Plasmid)
Size of vector backbone	4.2 kb
Selectable markers	Kan^r
Gene/insert name	Human hexokinase 2 genomic DNA
Depositor\|Developer	DNA Bank, \|

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB05882	pKM2L-phHKII	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pKM2L-phHKII was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05882).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB05882_A9Anp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 () Sequence file: RDB05882_A9Ana.seq
>05882_05882_A9An_3_M13_-40_A04_01_ABI08.ab1 1 AGGCCGAAGG CCAAGCCTTT GCATGCCTGC AGGTCATTTA AATAGGGATA ACAGGGTAAT 61 GATTAAGATC TCGAGCTCTA AGCTTCACAT ATGCATGCAC TAGTGGCATT GCAGACAGCA 121 CCCCACTGTT TGAAAAACTC CTCCTCAGTA GAACATGGCA GACCTTCATC TTCCTTCCCT 181 GAACCTTTTC CAACCTTAGG CTTGCCATTC TCCACCAGTG CTAATGTCAT GTCTCTTGAA 241 ATCTGTATTG AAGTCAGTAT TTCATTCTTG CCAGTTTCCA CTGTGTGTTT AAATTTGGAG 301 TCTGGTGTCT AGCATTAGCT GGGGTTGGGG CTTCCACTCC TCTCAGCATT GGTAAGCCTC 361 CTCACCCACC CCATCCCATG TCCAAGATCA CCCAGTTACA CACTTACCAT CTACCCAGTT 421 CATTCACATC ATCAGTCCCA GAGCTGCAGA GATGCTCTTT TTCTACCTCC TACTTCTCTG 481 GCTCTTAGAG AGGCAGCATG GGATAATGGG GCAAGCGAAT AGGGCCTTAA AGTAGAGGGA 541 CAAGGGTTCT CTTCCCTATC TGCCACTTAT TAGCTATGTG ACCTCGTGTA AGTCTCTTTT 601 CTTTTTGAGA CAGGGTCTCC CTCTGTCACC TAGGCTGGAG TACAGTGGTA TGATCATAGC 661 TCACTGCAGC CTCGAACTCC TGGGCTCAAG CTATCCTTCC ACCTTAGCCT TCTGAGCAGC 721 AGGGACTACA GGCACATGCC ACCATGTCCG GCTGATTTAT TTATTTTTAT TTGGGAAGAT 781 GGGGGTCTCA CTATGTCGCC CAGCTGGTCA TGAAACTCCT GGTCTCAAGC AACCCTCCAA 841 CTTTGGACTC CCAAAGTGCT GGGATTACAG GTGTGAGCCC TGGCCTTGCC TCAATTTCCT 901 CATCTGTAAA ACGGGGTTAG TGAAACTCAC A //
Primer: phRLR2 () Sequence file: RDB05882_A9Anb.seq
>05882_05882_A9An_3_phRLR2_A05_01_ABI08.ab1 1 GTTTCTCCCC GGGCCTCGAA AAGCCCAGGT TTCTATTGGT CTCCTTAAAC CTGTCTTGTA 61 ACCTTGATAC TTACCTGCCC AGTGCCTCAC GACCAACTTC TGCAGTACCC GGGTGAATTC 121 TACGCGTCGA CAGGCACAGG ATCATGAGGC TCAACTTGAG AAGAATCTCA GCCCGGCTCC 181 ACTCGGGCAG TGGGACCTCT CCGATTCACA GGCGCGTGGC TCGGGCCCGC GGATTTTCTT 241 AGCTGGGTGA CACGATGTTG CCAGCGGCAG CCCGGGACTC CTGCGCCGGA GTTTCATGCA 301 ACAATGTGGC TTATTGAGGG GCTGCCGGCC CGCGGGCCTC CGGATCAGTG ACGTGCGCAC 361 GCCCATTGGC TGCGCGGGGA GGGTGTGTGC GCCCACGGAC CGCCCGCCGC CGTGGCGCAG 421 CCAATCATCG CTCACGGCTC GCGGGCGCGG CCGCTCAGAC ACCGGAGCCG GCAGCCTGGG 481 GAGGCGCGCC GGGGGCGGGG GGCGGGGGAG GGGCGGGGCG CCTGAGATGG GACGTGTGGT 541 CCGTGGCTAA AGGCTGGGAC GGAAAGGCGC ATGAGCCACC GCCGCGTCAG GCTCGGCTAT 601 GGGTGGGGGG AGCGTGATCG CGGACCCGGG GTGTGGGGCG ACTGGAAAAA CGGGCCTCTG 661 GACCAGCACA CACCCTGTGC GG //
Primer: pAxCALNL_F1 () Sequence file: RDB05882_A9Anc.seq
>05882_05882_A9An_3_pAxCALNL_F1_C04_03_ABI08.ab1 1 CTCAAATTGT ATCTTTATCA TTGTCTGCTC GAAGCGGCCG CTCTAGAATT ACTGCTCGTT 61 CTTCAGCACG CGCTCCACGA AGCTCTTGAT GTACTTACCC ATTTCATCTG GAGCGTCCTC 121 CTGGCTGAAG TGGAGGCCCT TCACCTTCAC GAACTCGGTG TTAGGGAACT TCTTAGCTCC 181 CTCGACAATA GCGTTGGAAA AGAACCCAGG GTCGGACTCG ATGAACATCT TAGGCAGATC 241 GTCGCTGGCC CGAAGGTAGG CGTTGTAGTT GCGGACAATC TGGACGACGT CGGGCTTGCC 301 TCCCTTAACG AGAGGGATCT CGCGAGGCCA GGAGAGGGTA GGCCGTCTAA CCTCGCCCTT 361 CTCCTTGAAT GGCTCCAGGT AGGCAGCGAA CTCCTCAGGC TCCAGTTTCC GCATGATCTT 421 GCTTGGGAGC ATGGTCTCGA CGAAGAAGTT ATTCTCAAGC ACCATTTTCT CGCCCTCTTC 481 GCTCTTGATC AGGGCGATAT CCTCCTCGAT GTCAGGCCAC TCGTCCCAGG ACTCGATCAC 541 GTCCACGACA CTCTCAGCAT GGACGATGGC CTTGATCTTG TCTTGGTGCT CGTAGGAGTA 601 GTGAAAGGCC AGACAAGCCC CCCAGTCGTG GCCCACAAAG ATGATTTTCT TTGGAAGGTT 661 CAGCAGCTCG AACCAAGCGG TGAGGTACTT GTAGTGATCC AGGAGGCGAT ATGAGCCATT 721 CCCGCTCTTG CCGGACTTAC CCATTCCGAT CAGATCAGGG ATGATGCATC TAGCCACGGG 781 CTCGATGTGA GGCACGACGT GCCTCCACAG GTAGCTGGAG GCAGCGTTAC CATGCAGAAA 841 AAATCACGGC GTTCTCGGCG TGCTTCTCGG AATCATAGTA GTTGATGAAG GAGTCCAGCA 901 CGTTCATTTG CTTGCAGCGA GCCCACCACT GAGGCCCAGT GATCATGCGT TTGCGTTGCT 961 CGGGGTCGTA CACCTTGGAA GCCATGGTGG CTAGCCTAAT AGTGAGTCGT ATTAAGTACT 1021 CTAGCCTTTA GAGCTGTAAT TGAACTGGGA GTGGACCACC TTGTGGAGAG AAGGCAAAGT 1081 GGATGTCAGT TAGACCAATA GTGCCTATCA GAAAACCGCA AGAGTCTTCT CTGTTCTCGA 1141 ACAGCCAGTT TTCTATGATC TCCTTAAACC CTGGTCTGTA ACTGGATACT TACTGCCCAG 1201 TGCCCTCAGA CACTTCTGCA AGATAACCGG GTGTAACACT CG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

Resource search

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Other resources in our bank

human hexokinase 2

External Database
Human hexokinase 2

Reference sequence
AF148512