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pKM2L-phHKII

Promoter Bank clone, Human hexokinase II promoter

Catalog number RDB05882
Resource name pKM2L-phHKII
Alternative name p1899-19F
Clone info. PCR amplified human hexokinase II promoter sequence (Malkki, M. et al., Diabetologia, 40, 1461-1469, 1997, PMID:9447955.) was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 74,832,751 to 74,834,245 (NC_000002.12); relatively -1375 to +120, where +1 corresponds to 1 nt of XM_005264280.2; relatively +96 to +1591, where +1 corresponds to 1 nt of NM_000189.4.
Comment PCR cloning, forward primer, 1899 F2: 5' attgcagacagcaccccactgtt 3'; reverse primer, 1899 R2: 5' agtgggacctctccgattcacagg 3' Entire sequence of promoter region has not been confirmed.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human hexokinase 2 genomic DNA
Depositor|Developer DNA Bank, |
Other clones in our bank

External Database
Human hexokinase 2

          Reference sequence
            AF148512

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          RDB05882 pKM2L-phHKII DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Renilla Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB05882_A9Anp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40
          Sequence file: RDB05882_A9Ana.seq check
          >05882_05882_A9An_3_M13_-40_A04_01_ABI08.ab1
              1 AGGCCGAAGG CCAAGCCTTT GCATGCCTGC AGGTCATTTA AATAGGGATA ACAGGGTAAT
             61 GATTAAGATC TCGAGCTCTA AGCTTCACAT ATGCATGCAC TAGTGGCATT GCAGACAGCA
            121 CCCCACTGTT TGAAAAACTC CTCCTCAGTA GAACATGGCA GACCTTCATC TTCCTTCCCT
            181 GAACCTTTTC CAACCTTAGG CTTGCCATTC TCCACCAGTG CTAATGTCAT GTCTCTTGAA
            241 ATCTGTATTG AAGTCAGTAT TTCATTCTTG CCAGTTTCCA CTGTGTGTTT AAATTTGGAG
            301 TCTGGTGTCT AGCATTAGCT GGGGTTGGGG CTTCCACTCC TCTCAGCATT GGTAAGCCTC
            361 CTCACCCACC CCATCCCATG TCCAAGATCA CCCAGTTACA CACTTACCAT CTACCCAGTT
            421 CATTCACATC ATCAGTCCCA GAGCTGCAGA GATGCTCTTT TTCTACCTCC TACTTCTCTG
            481 GCTCTTAGAG AGGCAGCATG GGATAATGGG GCAAGCGAAT AGGGCCTTAA AGTAGAGGGA
            541 CAAGGGTTCT CTTCCCTATC TGCCACTTAT TAGCTATGTG ACCTCGTGTA AGTCTCTTTT
            601 CTTTTTGAGA CAGGGTCTCC CTCTGTCACC TAGGCTGGAG TACAGTGGTA TGATCATAGC
            661 TCACTGCAGC CTCGAACTCC TGGGCTCAAG CTATCCTTCC ACCTTAGCCT TCTGAGCAGC
            721 AGGGACTACA GGCACATGCC ACCATGTCCG GCTGATTTAT TTATTTTTAT TTGGGAAGAT
            781 GGGGGTCTCA CTATGTCGCC CAGCTGGTCA TGAAACTCCT GGTCTCAAGC AACCCTCCAA
            841 CTTTGGACTC CCAAAGTGCT GGGATTACAG GTGTGAGCCC TGGCCTTGCC TCAATTTCCT
          901 CATCTGTAAA ACGGGGTTAG TGAAACTCAC A
          //
          Primer: phRLR2
          Sequence file: RDB05882_A9Anb.seq check
          >05882_05882_A9An_3_phRLR2_A05_01_ABI08.ab1
              1 GTTTCTCCCC GGGCCTCGAA AAGCCCAGGT TTCTATTGGT CTCCTTAAAC CTGTCTTGTA
             61 ACCTTGATAC TTACCTGCCC AGTGCCTCAC GACCAACTTC TGCAGTACCC GGGTGAATTC
            121 TACGCGTCGA CAGGCACAGG ATCATGAGGC TCAACTTGAG AAGAATCTCA GCCCGGCTCC
            181 ACTCGGGCAG TGGGACCTCT CCGATTCACA GGCGCGTGGC TCGGGCCCGC GGATTTTCTT
            241 AGCTGGGTGA CACGATGTTG CCAGCGGCAG CCCGGGACTC CTGCGCCGGA GTTTCATGCA
            301 ACAATGTGGC TTATTGAGGG GCTGCCGGCC CGCGGGCCTC CGGATCAGTG ACGTGCGCAC
            361 GCCCATTGGC TGCGCGGGGA GGGTGTGTGC GCCCACGGAC CGCCCGCCGC CGTGGCGCAG
            421 CCAATCATCG CTCACGGCTC GCGGGCGCGG CCGCTCAGAC ACCGGAGCCG GCAGCCTGGG
            481 GAGGCGCGCC GGGGGCGGGG GGCGGGGGAG GGGCGGGGCG CCTGAGATGG GACGTGTGGT
            541 CCGTGGCTAA AGGCTGGGAC GGAAAGGCGC ATGAGCCACC GCCGCGTCAG GCTCGGCTAT
            601 GGGTGGGGGG AGCGTGATCG CGGACCCGGG GTGTGGGGCG ACTGGAAAAA CGGGCCTCTG
          661 GACCAGCACA CACCCTGTGC GG
          //
          Primer: pAxCALNL_F1
          Sequence file: RDB05882_A9Anc.seq check
          >05882_05882_A9An_3_pAxCALNL_F1_C04_03_ABI08.ab1
              1 CTCAAATTGT ATCTTTATCA TTGTCTGCTC GAAGCGGCCG CTCTAGAATT ACTGCTCGTT
             61 CTTCAGCACG CGCTCCACGA AGCTCTTGAT GTACTTACCC ATTTCATCTG GAGCGTCCTC
            121 CTGGCTGAAG TGGAGGCCCT TCACCTTCAC GAACTCGGTG TTAGGGAACT TCTTAGCTCC
            181 CTCGACAATA GCGTTGGAAA AGAACCCAGG GTCGGACTCG ATGAACATCT TAGGCAGATC
            241 GTCGCTGGCC CGAAGGTAGG CGTTGTAGTT GCGGACAATC TGGACGACGT CGGGCTTGCC
            301 TCCCTTAACG AGAGGGATCT CGCGAGGCCA GGAGAGGGTA GGCCGTCTAA CCTCGCCCTT
            361 CTCCTTGAAT GGCTCCAGGT AGGCAGCGAA CTCCTCAGGC TCCAGTTTCC GCATGATCTT
            421 GCTTGGGAGC ATGGTCTCGA CGAAGAAGTT ATTCTCAAGC ACCATTTTCT CGCCCTCTTC
            481 GCTCTTGATC AGGGCGATAT CCTCCTCGAT GTCAGGCCAC TCGTCCCAGG ACTCGATCAC
            541 GTCCACGACA CTCTCAGCAT GGACGATGGC CTTGATCTTG TCTTGGTGCT CGTAGGAGTA
            601 GTGAAAGGCC AGACAAGCCC CCCAGTCGTG GCCCACAAAG ATGATTTTCT TTGGAAGGTT
            661 CAGCAGCTCG AACCAAGCGG TGAGGTACTT GTAGTGATCC AGGAGGCGAT ATGAGCCATT
            721 CCCGCTCTTG CCGGACTTAC CCATTCCGAT CAGATCAGGG ATGATGCATC TAGCCACGGG
            781 CTCGATGTGA GGCACGACGT GCCTCCACAG GTAGCTGGAG GCAGCGTTAC CATGCAGAAA
            841 AAATCACGGC GTTCTCGGCG TGCTTCTCGG AATCATAGTA GTTGATGAAG GAGTCCAGCA
            901 CGTTCATTTG CTTGCAGCGA GCCCACCACT GAGGCCCAGT GATCATGCGT TTGCGTTGCT
            961 CGGGGTCGTA CACCTTGGAA GCCATGGTGG CTAGCCTAAT AGTGAGTCGT ATTAAGTACT
           1021 CTAGCCTTTA GAGCTGTAAT TGAACTGGGA GTGGACCACC TTGTGGAGAG AAGGCAAAGT
           1081 GGATGTCAGT TAGACCAATA GTGCCTATCA GAAAACCGCA AGAGTCTTCT CTGTTCTCGA
           1141 ACAGCCAGTT TTCTATGATC TCCTTAAACC CTGGTCTGTA ACTGGATACT TACTGCCCAG
          1201 TGCCCTCAGA CACTTCTGCA AGATAACCGG GTGTAACACT CG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl