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pAxit-phID2-rLuc (F) (#RDB05837)

Shuttle vector to generate recombinant adenovirus harboring renilla luciferase reporter.

Alternative name pAxp1934-9
Clone info. Shuttle vector to generate recombinant adenovirus harboring renilla luciferase reporter.
Comment Produce adenovirus by IT method.
Vector backbone pAxcwit (RDB3120) (Cosmid, use packaging extracts for transforming E. coli host)
Selectable markers Amp^r
Gene/insert name Human inhibitor of differentiation-2 (Id2) Genomic DNA
Depositor|Developer DNA Bank, | Murata, Takehide |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 　2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for not-for-profit research in the not-for-profit organization.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 1. 本件リソースは学術研究機関にのみ提供し、学術研究にのみ利用することができる。　2. 謝辞の表明を必要とする。 本件リソースは非営利機関にのみ提供し、非営利研究にのみ利用することができる。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB05837 pAxit-phID2-rLuc (F) Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pAxit-phID2-rLuc (F) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05837).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

reference Lasorella, A., Id2 is a retinoblastoma protein target and mediates signalling by Myc oncoproteins Nature 407 (6804): 592-598 (2000). PMID 11034201.
reference Kurabayashi, M., Sequences of the 5'-flanking region of the human helix-loop-helix protein-encoding Id2A gene, and promoter activity regulated by serum and c-Jun/AP-1. Gene 156 (2): 311-312 (1995). PMID 7758976.
reference Kurabayashi, M., Serum-inducible factors binding to an activating transcription factor motif regulate transcription of the Id2A promoter during myogenic differentiation. J. Biol. Chem. 269 (49): 31162-31170 (1994). PMID 7983058.