RDB05810 - pKM2L-phSPRR2F(2)

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pKM2L-phSPRR2F(2) (#RDB05810)

Promoter Bank clone, Human small proline-rich protein 2F (SPRR2F) promoter

Alternative name	p1998-57F
Clone info.	PCR amplified human small proline-rich protein 2F (SPRR2F) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 153116581 to 153113487(NC_000001.11); relatively -3065 to +30, where +1 corresponds to 1 nt of NM_001014450.3.
Comment	PCR cloning, forward primer, 1998 F2: 5' ctactcacgtccagccccacctttg 3'; reverse primer, 1998 R2: 5' gcagatcggtgcttaggtaccagga 3'; Entire sequence of promoter region has not been confirmed. Promoter activity has not been analyzed. (reference: J. Biol. Chem., 276, 19231-19237, 2001, PMID:11279051)
Vector backbone	pKM2L (RDB04026) (Plasmid)
Size of vector backbone	4.2 kb
Selectable markers	Kan^r
Gene/insert name	Human small proline-rich protein 2F genomic DNA
Depositor\|Developer	DNA Bank, \|

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB05810	pKM2L-phSPRR2F(2)	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

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How to cite this biological resource

Materials & Methods section:

The pKM2L-phSPRR2F(2) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05810).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB05810_A5Kkp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: PRE_rev Sequence file: RDB05810_A5Kka.seq
>05810_5810_A5Kk_M13(-40)_H05_23.ab1 1 AGGAAGGGTG CAAGGGCTTG CATGGCCTGC AGGTCATTTA AATAGGGATA ACAGGGTAAT 61 GATTAAGATC TCGAGCTCTA AGCTTCACAT ATGCATGCAC TAGTGGCCTA CTCACGTCCA 121 GCCCCACCTT TGGTGTTTGG CCAGAGATGC TTAAAAGGAA AAAGATCATT ATCTCACTAA 181 TTGAGCTATG GACAGACTTG TCTCTTGTTA GCTTTTCAAA GCAGAATTTT AAGTCTTTAC 241 AAAATAATAG GTAACATTTG GAGAATATTT TGAAAAGGAA AATAAGAAAA TAAAAATCAT 301 CTGTAATTAC ACCAACCAAA CAGAATCACA TTTAATAAAA TGTTTTAGGT ATTTCTTATC 361 TTTTCATACA TAAATGTTAA AATGTTGTCA TGCTTTATAT ATGCTTTTAC TATAATTTGA 421 ATGTTTTAAA TAATATTAAA TAGTCTTCTA AAACATCCTC CCTAATGATC TTAAGAATTC 481 TGCAACATTA ATGTCCCATA ATTTATCTGA TTAAGCAAAA TTATGGAATA ATGATGGAAT 541 GTAAGCCTAT ATTCAGGAAT AAAACAGAAG CAGAAATTCA CAGAGACTTA GAGTGATTTA 601 TTTAAGGCTA GGAAAATCTT CCCACAATTC CTGTTTCTTC TTGCATGTTT GATGGCAGAA 661 TATTAATTTG CCAGGACTAT TTTGTTTAAT GGTGTAAAAT GCATAGTAGT AAAATGTGAG 721 ATAGAAGTTG AATCAAGAAT AACACAAAAG TTCAAAAGCT TTTGTTCTTT CTTGTCATTT 781 TTATGTAGTA ATGATTTAAA CTTAATAATC CCTTCAAAAA TTATGGAGTA AAAGTAGGAG 841 AAATGGACCT AAGGCTATCA GACTGTTAAA GAACATAAAG CTAAACTTCT TTATATATGT 901 GGAGAGATAA TAGAAAGGTA GATGGATAGA TAGATGACAC ATTAGATAGA TAGAAGATAG 961 ATAGATAGAT AGATAGACAC ACACCCATGG TGGGTATGTG TGTGTGTGTA AAGTATGCCT 1021 TGCTGCATGT CTCAGGGCCC TGGATGTCCT AACTTGGGGA AGCTGTGACT GTTAGCTGCC 1081 CCCTCTTCAT GTGTGGCCAT TTCCTCATGT AGCCTGATCT GTGAGAAACT CTGAATTTCC 1141 AAGGTTCACT TGTAAAAGTT GAGAGCCTGG TCTCGTTGA //
Primer: PRE,mRFP1,T2A,TetR Sequence file: RDB05810_A5Kkb.seq
>05810_5810_A5Kk_phRLR2_B06_06.ab1 1 AATTTCTTTT TTCTGAAAGC CCAGTTTCTA TTGGTCTCCT TAAACCTGTC TTGTAACCTT 61 GATACTTACC TGCCCAGTGC CTCACGACCA ACTTCTGCAG TACCCGGGTG AATTCTACGC 121 GTCGACAGGC GCAGATCGGT GCTTAGGTAC CAGGAGTTTA GGAGTCGTGC AGCAGAAAAC 181 CTCTTTATAG GGCCTGCTAC CCCTCCCAGC AGAAAGTGTA ACTGCCAAAA GCTACACTTA 241 TCCAGTAATT TCTTAAGCCA AATGCAAATT TATCCACAAC TGGCCTGACA GTGATTATCA 301 AACTAGGAAA TATCCTGTTC TGGGATCTCC CCACTGGGTT AAGTGCTCCT GACTCACAGA 361 GGCCCTGCCT TAGATCTCAG TTTCAGTGAC CTATGGCAAA GAGGGACTTG GGGGATTCTC 421 TAACTGGTGG TCAAGGGCTC TCTTTTTTGA CTGGGGGCAA TGATCCTTTC CTGTCTTACA 481 CCTTCCCTAA ATCATAAAAC TTCCTGTCCT GATCCACTTT ACTGAATGCC ATATTGTTGT 541 ACATGAACCC ATCTCCACCT CTACTTTCCT TGTACAATTT GCCAATGCGC TAAAAGGAAG 601 GAGAGACCTA ACAAAGGCCT GGGTTCTGGT CCCAGATTTT TTTTTTTTTT TTTTTTTTTT 661 TTTTGTTGCT AAGTTGTGTG ACCACAGGCA GGTCACCTCT CCTCCCTGTG ATTATAAGCA 721 GGGGAGAATC ATACTTACCT CTGGAGATGG GAGCTGACCG CATTGTGCCA TCTGCTGACC 781 CTGTTCAAGC CAGAAACTTT CTCCTCCACT TCTATCTTTC TCTCTCATAG AACCCTGGAT 841 TTTACGATTT TCCTTTCCTC TTTGAAAGCT GTCTTCTCCA CCCCAATTTG TACAGGCAAT 901 TGTCACCTGG GACAGATTCC AGAGTGACAG CCAGTCTCCC CAGCACATTT GCTACTTGCT 961 GCCATTAAGG ATGTATACTA GCGCCTTTTT ATTTATAAAA ATGTATTCTT TGCTACACAT 1021 TAAGGGAAAC GGGAGAGACA TGAAACAAAA TTACAGCATT CATTCAAAAT CTTATGTTAT 1081 TGAACATGCT ATATGATCCT GCGCTACTAT AGAGAACAAT CTGGCATAGA CCTCACATTA 1141 TCCATATGGG TGCAAATAAA CTG //
Primer: pAxCALNL_F1 Sequence file: RDB05810_A5Kkc.seq
>05810_5810_A5Kk_pAxCALNL_F1_A06_03.ab1 1 ACAATATAAT CTTATCATGT CTGCTCGAGC GGCCGCTCTA GAATTACTGC TCGTTCTTCA 61 GCACGCGCTC CACGAAGCTC TTGATGTACT TACCCATTTC ATCTGGAGCG TCCTCCTGGC 121 TGAAGTGGAG GCCCTTCACC TTCACGAACT CGGTGTTAGG GAACTTCTTA GCTCCCTCGA 181 CAATAGCGTT GGAAAAGAAC CCAGGGTCGG ACTCGATGAA CATCTTAGGC AGATCGTCGC 241 TGGCCCGAAG GTAGGCGTTG TAGTTGCGGA CAATCTGGAC GACGTCGGGC TTGCCTCCCT 301 TAACGAGAGG GATCTCGCGA GGCCAGGAGA GGGTAGGCCG TCTAACCTCG CCCTTCTCCT 361 TGAATGGCTC CAGGTAGGCA GCGAACTCCT CAGGCTCCAG TTTCCGCATG ATCTTGCTTG 421 GGAGCATGGT CTCGACGAAG AAGTTATTCT CAAGCACCAT TTTCTCGCCC TCTTCGCTCT 481 TGATCAGGGC GATATCCTCC TCGATGTCAG GCCACTCGTC CCAGGACTCG ATCACGTCCA 541 CGACACTCTC AGCATGGACG ATGGCCTTGA TCTTGTCTTG GTGCTCGTAG GAGTAGTGAA 601 AGGCCAGACA AGCCCCCCAG TCGTGGCCCA CAAAGATGAT TTTCTTTGGA AGGTTCAGCA 661 GCTCGAACCA AGCGGTGAGG TACTTGTAGT GATCCAGGAG GCGATATGAG CCATTCCCGC 721 TCTTGCCGGA CTTACCCATT CCGATCAGAT CAGGGATGAT GCATCTAGCC ACGGGCTCGA 781 TGTGAGGCAC GACGTGCCTC CACAGGTAGC TGGAGGCAGC GTTACCATGC AGAAAAATCA 841 CGGCGTTCTC GGCGTGCTTC TCGGAATCAT AGTAGTTGAT GAAGGAGTCC AGCACGTTCA 901 TTTGCTTGCA GCGAGCCCAC CACTGAGGCC CAGTGATCAT GCGTTTGCGT TGCTCGGGGT 961 CGTACACCTT GGAAGCCATG GTGGCTAGCC TATAGTGAGT CGTATTAAGT ACTCTAGCCC 1021 TTAGAGCTGT AATTGAACTG GGAGTGACAC CTGTGAGAGA AAGGCAAAGT GAATGGTCAG 1081 TAGACCATAG TGCCTATCAG AACGCAGAGT CTCCTCTTGT CTCGACAGCC AGGTTCCATG 1141 TCTCGTAACT GATCTGTAAC //