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CS-RfA-CMV-mRFP1

Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with mRFP1 marker driven by CMV promoter.

Catalog number RDB05747
Resource name CS-RfA-CMV-mRFP1
Clone info. Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with mRFP1 marker driven by CMV promoter.
Comment Commonly requested with pENTR4-H1 (RDB04395) for generation of shRNA expression vector and with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394) for packaging. Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CS-RfA-CMV-mRFP1 (Plasmid)
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Miyoshi, Hiroyuki |
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External Database

            Reference sequence
              
             
            Sequence RDB05747z.seq
            Remarks, protocol and/or map (pdf) RDB05747.pdf
            Lentiviral plasmid list. [link]

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
            Ordering Please visit Ordering instruction.[link] 
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            Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
            Remarks Remember that you will be working with samples containing infectious virus.
            Use Survival2 (Invitrogen) or equivalent to amplify this clone.
            This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
            提供案内 (日本国内) [open/close]

            提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
            提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (非営利機関による非営利学術研究用)[Word]
            遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
            備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
            このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。
            Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB05747 CS-RfA-CMV-mRFP1 DNA solution

            Ordering instruction of plasmids [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The CS-RfA-CMV-mRFP1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05747).

            Reference section:

            Further references such as user reports and related articles (go to bottom)


            References and tips

            Featured content

            Featured content Lentivirus Vector (English text)
            Featured content Lentivirus Vector (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12274_A6Gdp1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: Cm_M_R
            Sequence file: RDB12274_A6Gda.seq check
            >05747_12274_A6Gd_Cm_M_R_D02_11.ab1
                1 GCTTGAAAAG ACCTATCCAT ATCACCAGCT CACCGTCTTT CATTGCCATA CGGAATTCCG
               61 GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC TTGTGCTTAT
              121 TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG TTATAGGTAC
              181 ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG GATATATCAA
              241 CGGTGGTATA TCCAGTGATT TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT GAAAATCTCG
              301 ACGGATCCTA ACTCAAAATC CACACATTAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG
              361 GGGTGCCTAA TGCGGCCGCC ATAGTGACTG GATATGTTGT GTTTTACAGT ATTATGTAGT
              421 CTGTTTTTTA TGCAAAATCT AATTTAATAT ATTGATATTT ATATCATTTT ACGTTTCTCG
              481 TTCAGCTTTT TTGTACAAAC TTGTGATAAT TCAAAGTGAA TTTCTGCTGT CCCTGTAATA
              541 AACCCGAAAA TTTTGAATTT TTGTAATTTG TTTTTGTAAT TCTTTAGTTT GTATGTCTGT
              601 TGCTATTATG TCTACTATTC TTTCCCCTGC ACTGTACCCC CCAATCCCCC CTTTTCTTTT
              661 AAAATTGTGG ATGAATACTG CCATTTGTAG ATCCGTTCAC TAATCGAATG GATCTGTCTC
              721 TGTCTCTCTC TCCACCTTCT TCTTCTATTC CTTCGGGCCT GTCGGGTCCC CTCGGGGTTG
              781 GGAGGTGGGT CTGAAACGAT AATGGTGAAT ATCCCTGCCT AACTCTATTC ACTATAGAAA
              841 AGTACAGCAA AAAACTATTC TTAAACCTAC CAAGCCCTCC TACTATCATT ATGAATAATT
              901 TTATATACCA CAGCCCAATT TGTTATGTTA AACCAATTCC ACAAACTTGC CCATTTATCT
              961 AATTCCCAAT AATTTCTTGT TCATTCTTTT CTTGCTGGGT TTTTGCGATT CTTCAATTAA
             1021 GAGTGTAATT TAAGCTTTGT GGTATGATAA TTTCTTCTTG ATCCCACTCC AATCAAGGTC
            1081 GATGTGATTC CAATCTGGAT TTCG
            //
            Primer: Cm_C_F
            Sequence file: RDB12274_A6Gdb.seq check
            >05747_12274_A6Gd_Cm-C_F_C12_09.ab1
                1 GGGCCTAACC GTGGTCCGGG GCTTACTAAA GCCAGATAAC AGTATGCGTA TTTGCGCGCT
               61 GATTTTTGCG GTATAAGAAT ATATACTGAT ATGTATACCC GAAGTATGTC AAAAAGAGGT
              121 ATGCTATGAA GCAGCGTATT ACAGTGACAG TTGACAGCGA CAGCTATCAG TTGCTCAAGG
              181 CATATATGAT GTCAATATCT CCGGTCTGGT AAGCACAACC ATGCAGAATG AAGCCCGTCG
              241 TCTGCGTGCC GAACGCTGGA AAGCGGAAAA TCAGGAAGGG ATGGCTGAGG TCGCCCGGTT
              301 TATTGAAATG AACGGCTCTT TTGCTGACGA GAACAGGGGC TGGTGAAATG CAGTTTAAGG
              361 TTTACACCTA TAAAAGAGAG AGCCGTTATC GTCTGTTTGT GGATGTACAG AGTGATATTA
              421 TTGACACGCC CGGGCGACGG ATGGTGATCC CCCTGGCCAG TGCACGTCTG CTGTCAGATA
              481 AAGTCTCCCG TGAACTTTAC CCGGTGGTGC ATATCGGGGA TGAAAGCTGG CGCATGATGA
              541 CCACCGATAT GGCCAGTGTG CCGGTCTCCG TTATCGGGGA AGAAGTGGCT GATCTCAGCC
              601 ACCGCGAAAA TGACATCAAA AACGCCATTA ACCTGATGTT CTGGGGAATA TAAATGTCAG
              661 GCTCCCTTAT ACACAGCCAG TCTGCAGGTC GACCATAGTG ACTGGATATG TTGTGTTTTA
              721 CAGTATTATG TAGTCTGTTT TTTATGCAAA ATCTAAATTT AAATATATTG ATATTTATAT
              781 CATTTTACGT TTCTCGTTCA GCTTTCTTGT ACAAAGTGGT GATAATTCGA TTAATAGTAA
              841 TCAATTTACG GGGTCATTAG TTCATAGCCC ATATATGGGA GTTCCCGCGT TTACATAACT
              901 TACGGTAAAA TGGCCCGCCT GGCTTGACCG CCCAACGAAC CCCCCGCCCA TTGACGTTCA
              961 ATAAATGAAC GTTATGTTTC CCCATAGTAA ACGGCCAAAT TAGGGACTTT TCCCATTTGA
             1021 CGGTTCAATG GGGTTGGAGG TTATTTTTTA CGGTTAAACC TGGCCCCAAC TTGGGCCAGT
             1081 ACATTCAAGT TGTATTCCAT ATTGCCCAAA GGTTACGGCC CCCCGTATTT GAACGATCCA
            1141 ATTGAAACAG
            //
            Primer: PRE_rev
            Sequence file: RDB12274_A6Gdc.seq check
            >05747_12274_A6Gd_PRE_rev_D12_12.ab1
                1 AGAACCCATA GCGTAAAGGA GCAACATAGT TAAGAATACC AGTCAATCTT TCACAAATTT
               61 TGTAATCCAG AGGTTGATTA TCGATAAGCT TGATCCCTCG AAATTCTTAG GCGCCGGTGG
              121 AGTGGCGGCC CTCGGCGCGC TCGTACTGTT CCACGATGGT GTAGTCCTCG TTGTGGGAGG
              181 TGATGTCCAG CTTGATGTCG GTCTTGTAGG CGCCGGGCAG CTGCACGGGC TTCTTGGCCA
              241 TGTAGGTGGT CTTGACCTCG GCGTCGTAGT GGCCGCCGTC CTTCAGCTTC AGCCTCATCT
              301 TGATCTCGCC CTTCAGGGCG CCGTCCTCGG GGTACATCCG CTCGGTGGAG GCCTCCCAGC
              361 CCATGGTCTT CTTCTGCATT ACGGGGCCGT CGGAGGGGAA GTTGGTGCCG CGCAGCTTCA
              421 CCTTGTAGAT GAACTCGCCG TCCTGCAGGG AGGAGTCCTG GGTCACGGTC ACCACGCCGC
              481 CGTCCTCGAA GTTCATCACG CGCTCCCACT TGAAGCCCTC GGGGAAGGAC AGCTTCAAGT
              541 AGTCGGGGAT GTCGGCGGGG TGCTTCACGT AGGCCTTGGA GCCGTACTGG AACTGAGGGG
              601 ACAGGATGTC CCAGGCGAAG GGCAGGGGGC CGCCCTTGGT CACCTTCAGC TTGGCGGTCT
              661 GGGTGCCCTC GTAGGGGCGG CCCTCGCCCT CGCCCTCGAT CTCGAACTCG TGGCCGTTCA
              721 CGGAGCCCTC CATGCGCACC TTGAAAGCGC ATGAACTCCC TTGATGACGG TCCCTCGGAG
              781 GAGGCCATTC GGGATCGCTA GCGGATCTGA ACGGGTTCAC TAAACCAGCC TCTGCTTATA
              841 TAAGACCTTC CCAACGTAAC ACGCCGACCG GCCCAATTGG CGTTCAAATG GGGCGGGAGT
              901 TTGTTACGAC ATTTTTGGAA AGGTCCCCGG TGAATTTTGG GTGCCCAAAA ACAACTTCCC
              961 CATTGACCGT CAATGGGGGT GGAGTACTTT GGGAATAACC CCCGGTGGAA GTCCAAAACC
            1021 CGACCTATAT CCGCCACGCC CGCAATGTGA
            //
            Primer: HIVgp_R1
            Sequence file: RDB12274_A6Gdd.seq check
            >05747_12274_A6Gd_HIVgp_R1_E12_15.ab1
                1 CCGGACATTC TATTTCCCAC TGCTCTTTTT TCTCTCTGCA CCACTCTTCT CTTTGCCTTG
               61 GTGGGTGCTA CTCCTAATGG TTCAATTTTT ACTACTTTAT ATTTATATAA TTCACTTCTC
              121 CAATTGTCCC TCATATCTCC TCCTCCAGGT CTGAAGATCA GCGGCCGCTT GCTGTGCGGT
              181 GGTCTTACTT TTGTTTTGCT CTTCCTCTAT CTTGTCTAAA GCTTCCTTGG TGTCTTTTAT
              241 CTCTATCCTT TGATGCACAC AATAGAGGGT TGCTACTGTA TTATATAATG ATCTAAGTTC
              301 TTCTGATCCT GTCTGAAGGG ATGGTTGTAG CTGTCCCAGT ATTTGTCTAC AGCCTTCTGA
              361 TGTTTCTAAC AGGCCAGGAT TAACTGCGAA TCGTTCTAGC TCCCTGCTTG CCCATACTAT
              421 ATGTTTTAAT TTATATTTTT TCTTTCCCCC TGGCCTTAAC CGAATTTTTT CCCATCGCGA
              481 TCTAATTCTC CCCCGCTTAA TACTGACGCT CTCGCACCCA TCTCTCTCCT TCTAGCCTCC
              541 GCTAGTCAAA ATTTTTGGCG TACTCACCAG TCGCCGCCCC TCGCCTCTTG CCGTGCGCGC
              601 TTCAGCAAGC CGAGTCCTGC GTCGAGAGAG CTCCTCTGGT TTCCCTTTCG CTTTCAAGTC
              661 CCTGTTCGGG CGCCACTGCT AGAGATTTTC CACACTGACT AAAAGGGTCT GAGGGATCTC
              721 TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT GAAGCACTCA AGGCAAGCTT
              781 TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA GCTCCCAGGC TCAGATCTGG
              841 TCTAACCAGA GAGACCCAGT ACAGGCAAAA CGCGCTGCTT ATATAGACCT CCCACCGTAC
              901 ACGCCTACCG CCCATTTGCG TCAATGGGGG CGGAGTTGTT ACGACATTTT GGAAAGTCCC
              961 GTTTGATTTT GGGTGCCCAA AACAAACTCC CATTGAACGT CAATGGGGTG GAGACTTTGG
             1021 AAAATCCCCC GTGAGTCAAA CCGGCTATCC ACGCCCATTG GATGTACTTG CCAAAATCGC
            1081 AATTCATCAT GGTTAAATAA GCGATTGACT AAATAACCGT
            //
            Primer: BGH_rev2
            Sequence file: RDB12274_A6Gde.seq check
            >05747_12274_A6Gd_BGH_rev2_F12_18.ab1
                1 CATCGGGTTA GTAGCACGGG GGAGGGGCAA CAACAGATGG CTGGCAACTA GAAGGCACAG
               61 TCGAGGCTGA TCAGCGGGTT TAAACGGGCC CTGCTAGAGA TTTTCCACAC TGACTAAAAG
              121 GGTCTGAGGG ATCTCTAGTT ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC
              181 ACTCAAGGCA AGCTTTATTG AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC
              241 CAGGCTCAGA TCTGGTCTAA CCAGAGAGAC CCAGTACAGT CCGGATGCAG CTCTCGGGCC
              301 ATGTGATGAA ATGCTAGGCG GCTGTCAAAC CTCCACTCTA ATACTTCTCT CTCCGGGTCA
              361 TCCATCCCAT GCAGGCTCAC AGGGTGTAAC AAGCGGGTGT TCTCTCCTTC ATTGGCTTCT
              421 TCTACCTTCT CTTGCTCAAC TGGTACTAGC TTGTAGCACC ATCCAAAGGT CAGTGGATAT
              481 CTGATCCCTG GCCCTGGTGT GTAGTTCTGC CAATCAGGGA AGTAGCCTTG TGTGTGGTAG
              541 ATCCACAGAT CAAGGATATC TTGTCTTCGT TGGGAGTGAA TTAGCCCTTC CAGTCCCCCC
              601 TTTTCTTTTA AAAAGTGGCT AAGATCTACA GCTGCCTTGT AAGTCATTGG TCTTAAAGGT
              661 ACCTGAGGTG TGACTGGAAA ACCCACCTCC TCCTCCTCTT GTGCTTCTAG CCAGGCACAA
              721 TCAGCATTGG TAGCTGCTGT ATTGCTACTT GTGATTGCTC CATGTTTTTC TAGGTCTCGA
              781 CGGTATCGAT GCGGGGAGGC GGCCCAAAGG GAGATCCGAC TCGTCTGAGG GCGAAGGCGA
              841 AGACGCGGAA GAGGCCGCAG AGCCGGCAGC AGCCGCGGGA AGGAAAGGTC CGCTGGATTG
              901 AGGGCCGATG GGACGTAGCA GAAGGACGTC CCGCGCAGAA TCCAGGTGGC AACACAGGCG
              961 AGCAGCCATG GAAAGGACGT CAGCTTTCCC CGACAACACC ACGGGAATTG GTCAGTGCCC
             1021 ACAGCCGAGC CCCTGGTCCA AGCAGCGGCA GCCAGGCGCG AATGAGTACG TCGTTGCCAT
            1081 AGGAGGGGCA AAGCCGGA
            //
            Primer: SV40pro_F
            Sequence file: RDB12274_A6Gdf.seq check
            >05747_12274_A6Gd_SV40pro_F_G12_21.ab1
                1 GCTGGTCCCT TTGACATCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
               61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAGCACGTGT TGACAATTAA
              121 TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA AACCATGGCC
              181 AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG TCGCCGGAGC GGTCGAGTTC
              241 TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG ACGACTTCGC CGGTGTGGTC
              301 CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC AGGTGGTGCC GGACAACACC
              361 CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG CCGAGTGGTC GGAGGTCGTG
              421 TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG AGATCGGCGA GCAGCCGTGG
              481 GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG TGCACTTCGT GGCCGAGGAG
              541 CAGGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC
              601 TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG
              661 GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT
              721 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC
              781 AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG
              841 TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC
              901 ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA
              961 TTAAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGAA ACCCTGGTCG TGCCAGCTGC
             1021 CATTAAATGA ATCGCCAAAC GCCGCGGGAG AGGGCGATTG CGTATTGGCG CTCTTTCCGC
            1081 TTTTC
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            user_report Funato, K., SIRT2-mediated inactivation of p73 is required for glioblastoma tumorigenicity. EMBO Rep. 19 (11). pii: e45587 (2018). PMID 30213795.
            user_report Maishi, N., Tumour endothelial cells in high metastatic tumours promote metastasis via epigenetic dysregulation of biglycan. Sci. Rep. 6: 28039 (2016). PMID 27295191.
            user_report Sasaki, M., Equine major histocompatibility complex class I molecules act as entry receptors that bind to equine herpesvirus-1 glycoprotein D. Genes Cells, 16 (4), 343-357 (2011). PMID 21306483.

            2023.05.02

            GNP_filter3_RDBDEP_html_230501.pl