Resource data sheet
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CS-RfA-CMV-mRFP1

Shuttle vector to generate recombinant Lentiviral vector expressing RFP by Gateway(R) system.

Catalog number RDB05747
Resource name CS-RfA-CMV-mRFP1
Clone info. Shuttle vector to generate recombinant Lentiviral vector. Gateway(R) destination vector with RFP as a marker.
Use DB3.1 (Invitrogen) or equivalent to amplify this clone. Commonly requested with pENTR4-H1 (RDB04395) for generation of shRNA expression vector and with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394) for packaging.
Vector backbone Plasmid
Originator Miyoshi, Hiroyuki |

Distribution information

CLICK here for plasmid list and map. [link]
Forms for plasmid request [in Japanese] [in English]
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
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Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus. Please contact us. We will provide you with appropriate documents.
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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12274_A6Gdp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Cm_M_R RDB12274_A6Gda.seq
>05747_12274_A6Gd_Cm_M_R_D02_11.ab1
    1 GCTTGAAAAG ACCTATCCAT ATCACCAGCT CACCGTCTTT CATTGCCATA CGGAATTCCG
   61 GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC TTGTGCTTAT
  121 TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG TTATAGGTAC
  181 ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG GATATATCAA
  241 CGGTGGTATA TCCAGTGATT TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT GAAAATCTCG
  301 ACGGATCCTA ACTCAAAATC CACACATTAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG
  361 GGGTGCCTAA TGCGGCCGCC ATAGTGACTG GATATGTTGT GTTTTACAGT ATTATGTAGT
  421 CTGTTTTTTA TGCAAAATCT AATTTAATAT ATTGATATTT ATATCATTTT ACGTTTCTCG
  481 TTCAGCTTTT TTGTACAAAC TTGTGATAAT TCAAAGTGAA TTTCTGCTGT CCCTGTAATA
  541 AACCCGAAAA TTTTGAATTT TTGTAATTTG TTTTTGTAAT TCTTTAGTTT GTATGTCTGT
  601 TGCTATTATG TCTACTATTC TTTCCCCTGC ACTGTACCCC CCAATCCCCC CTTTTCTTTT
  661 AAAATTGTGG ATGAATACTG CCATTTGTAG ATCCGTTCAC TAATCGAATG GATCTGTCTC
  721 TGTCTCTCTC TCCACCTTCT TCTTCTATTC CTTCGGGCCT GTCGGGTCCC CTCGGGGTTG
  781 GGAGGTGGGT CTGAAACGAT AATGGTGAAT ATCCCTGCCT AACTCTATTC ACTATAGAAA
  841 AGTACAGCAA AAAACTATTC TTAAACCTAC CAAGCCCTCC TACTATCATT ATGAATAATT
  901 TTATATACCA CAGCCCAATT TGTTATGTTA AACCAATTCC ACAAACTTGC CCATTTATCT
  961 AATTCCCAAT AATTTCTTGT TCATTCTTTT CTTGCTGGGT TTTTGCGATT CTTCAATTAA
 1021 GAGTGTAATT TAAGCTTTGT GGTATGATAA TTTCTTCTTG ATCCCACTCC AATCAAGGTC
1081 GATGTGATTC CAATCTGGAT TTCG
//
Primer: Cm_C_F RDB12274_A6Gdb.seq
>05747_12274_A6Gd_Cm-C_F_C12_09.ab1
    1 GGGCCTAACC GTGGTCCGGG GCTTACTAAA GCCAGATAAC AGTATGCGTA TTTGCGCGCT
   61 GATTTTTGCG GTATAAGAAT ATATACTGAT ATGTATACCC GAAGTATGTC AAAAAGAGGT
  121 ATGCTATGAA GCAGCGTATT ACAGTGACAG TTGACAGCGA CAGCTATCAG TTGCTCAAGG
  181 CATATATGAT GTCAATATCT CCGGTCTGGT AAGCACAACC ATGCAGAATG AAGCCCGTCG
  241 TCTGCGTGCC GAACGCTGGA AAGCGGAAAA TCAGGAAGGG ATGGCTGAGG TCGCCCGGTT
  301 TATTGAAATG AACGGCTCTT TTGCTGACGA GAACAGGGGC TGGTGAAATG CAGTTTAAGG
  361 TTTACACCTA TAAAAGAGAG AGCCGTTATC GTCTGTTTGT GGATGTACAG AGTGATATTA
  421 TTGACACGCC CGGGCGACGG ATGGTGATCC CCCTGGCCAG TGCACGTCTG CTGTCAGATA
  481 AAGTCTCCCG TGAACTTTAC CCGGTGGTGC ATATCGGGGA TGAAAGCTGG CGCATGATGA
  541 CCACCGATAT GGCCAGTGTG CCGGTCTCCG TTATCGGGGA AGAAGTGGCT GATCTCAGCC
  601 ACCGCGAAAA TGACATCAAA AACGCCATTA ACCTGATGTT CTGGGGAATA TAAATGTCAG
  661 GCTCCCTTAT ACACAGCCAG TCTGCAGGTC GACCATAGTG ACTGGATATG TTGTGTTTTA
  721 CAGTATTATG TAGTCTGTTT TTTATGCAAA ATCTAAATTT AAATATATTG ATATTTATAT
  781 CATTTTACGT TTCTCGTTCA GCTTTCTTGT ACAAAGTGGT GATAATTCGA TTAATAGTAA
  841 TCAATTTACG GGGTCATTAG TTCATAGCCC ATATATGGGA GTTCCCGCGT TTACATAACT
  901 TACGGTAAAA TGGCCCGCCT GGCTTGACCG CCCAACGAAC CCCCCGCCCA TTGACGTTCA
  961 ATAAATGAAC GTTATGTTTC CCCATAGTAA ACGGCCAAAT TAGGGACTTT TCCCATTTGA
 1021 CGGTTCAATG GGGTTGGAGG TTATTTTTTA CGGTTAAACC TGGCCCCAAC TTGGGCCAGT
 1081 ACATTCAAGT TGTATTCCAT ATTGCCCAAA GGTTACGGCC CCCCGTATTT GAACGATCCA
1141 ATTGAAACAG
//
Primer: PRE_rev RDB12274_A6Gdc.seq
>05747_12274_A6Gd_PRE_rev_D12_12.ab1
    1 AGAACCCATA GCGTAAAGGA GCAACATAGT TAAGAATACC AGTCAATCTT TCACAAATTT
   61 TGTAATCCAG AGGTTGATTA TCGATAAGCT TGATCCCTCG AAATTCTTAG GCGCCGGTGG
  121 AGTGGCGGCC CTCGGCGCGC TCGTACTGTT CCACGATGGT GTAGTCCTCG TTGTGGGAGG
  181 TGATGTCCAG CTTGATGTCG GTCTTGTAGG CGCCGGGCAG CTGCACGGGC TTCTTGGCCA
  241 TGTAGGTGGT CTTGACCTCG GCGTCGTAGT GGCCGCCGTC CTTCAGCTTC AGCCTCATCT
  301 TGATCTCGCC CTTCAGGGCG CCGTCCTCGG GGTACATCCG CTCGGTGGAG GCCTCCCAGC
  361 CCATGGTCTT CTTCTGCATT ACGGGGCCGT CGGAGGGGAA GTTGGTGCCG CGCAGCTTCA
  421 CCTTGTAGAT GAACTCGCCG TCCTGCAGGG AGGAGTCCTG GGTCACGGTC ACCACGCCGC
  481 CGTCCTCGAA GTTCATCACG CGCTCCCACT TGAAGCCCTC GGGGAAGGAC AGCTTCAAGT
  541 AGTCGGGGAT GTCGGCGGGG TGCTTCACGT AGGCCTTGGA GCCGTACTGG AACTGAGGGG
  601 ACAGGATGTC CCAGGCGAAG GGCAGGGGGC CGCCCTTGGT CACCTTCAGC TTGGCGGTCT
  661 GGGTGCCCTC GTAGGGGCGG CCCTCGCCCT CGCCCTCGAT CTCGAACTCG TGGCCGTTCA
  721 CGGAGCCCTC CATGCGCACC TTGAAAGCGC ATGAACTCCC TTGATGACGG TCCCTCGGAG
  781 GAGGCCATTC GGGATCGCTA GCGGATCTGA ACGGGTTCAC TAAACCAGCC TCTGCTTATA
  841 TAAGACCTTC CCAACGTAAC ACGCCGACCG GCCCAATTGG CGTTCAAATG GGGCGGGAGT
  901 TTGTTACGAC ATTTTTGGAA AGGTCCCCGG TGAATTTTGG GTGCCCAAAA ACAACTTCCC
  961 CATTGACCGT CAATGGGGGT GGAGTACTTT GGGAATAACC CCCGGTGGAA GTCCAAAACC
1021 CGACCTATAT CCGCCACGCC CGCAATGTGA
//
Primer: HIVgp_R1 RDB12274_A6Gdd.seq
>05747_12274_A6Gd_HIVgp_R1_E12_15.ab1
    1 CCGGACATTC TATTTCCCAC TGCTCTTTTT TCTCTCTGCA CCACTCTTCT CTTTGCCTTG
   61 GTGGGTGCTA CTCCTAATGG TTCAATTTTT ACTACTTTAT ATTTATATAA TTCACTTCTC
  121 CAATTGTCCC TCATATCTCC TCCTCCAGGT CTGAAGATCA GCGGCCGCTT GCTGTGCGGT
  181 GGTCTTACTT TTGTTTTGCT CTTCCTCTAT CTTGTCTAAA GCTTCCTTGG TGTCTTTTAT
  241 CTCTATCCTT TGATGCACAC AATAGAGGGT TGCTACTGTA TTATATAATG ATCTAAGTTC
  301 TTCTGATCCT GTCTGAAGGG ATGGTTGTAG CTGTCCCAGT ATTTGTCTAC AGCCTTCTGA
  361 TGTTTCTAAC AGGCCAGGAT TAACTGCGAA TCGTTCTAGC TCCCTGCTTG CCCATACTAT
  421 ATGTTTTAAT TTATATTTTT TCTTTCCCCC TGGCCTTAAC CGAATTTTTT CCCATCGCGA
  481 TCTAATTCTC CCCCGCTTAA TACTGACGCT CTCGCACCCA TCTCTCTCCT TCTAGCCTCC
  541 GCTAGTCAAA ATTTTTGGCG TACTCACCAG TCGCCGCCCC TCGCCTCTTG CCGTGCGCGC
  601 TTCAGCAAGC CGAGTCCTGC GTCGAGAGAG CTCCTCTGGT TTCCCTTTCG CTTTCAAGTC
  661 CCTGTTCGGG CGCCACTGCT AGAGATTTTC CACACTGACT AAAAGGGTCT GAGGGATCTC
  721 TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT GAAGCACTCA AGGCAAGCTT
  781 TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA GCTCCCAGGC TCAGATCTGG
  841 TCTAACCAGA GAGACCCAGT ACAGGCAAAA CGCGCTGCTT ATATAGACCT CCCACCGTAC
  901 ACGCCTACCG CCCATTTGCG TCAATGGGGG CGGAGTTGTT ACGACATTTT GGAAAGTCCC
  961 GTTTGATTTT GGGTGCCCAA AACAAACTCC CATTGAACGT CAATGGGGTG GAGACTTTGG
 1021 AAAATCCCCC GTGAGTCAAA CCGGCTATCC ACGCCCATTG GATGTACTTG CCAAAATCGC
1081 AATTCATCAT GGTTAAATAA GCGATTGACT AAATAACCGT
//
Primer: BGH_rev2 RDB12274_A6Gde.seq
>05747_12274_A6Gd_BGH_rev2_F12_18.ab1
    1 CATCGGGTTA GTAGCACGGG GGAGGGGCAA CAACAGATGG CTGGCAACTA GAAGGCACAG
   61 TCGAGGCTGA TCAGCGGGTT TAAACGGGCC CTGCTAGAGA TTTTCCACAC TGACTAAAAG
  121 GGTCTGAGGG ATCTCTAGTT ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC
  181 ACTCAAGGCA AGCTTTATTG AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC
  241 CAGGCTCAGA TCTGGTCTAA CCAGAGAGAC CCAGTACAGT CCGGATGCAG CTCTCGGGCC
  301 ATGTGATGAA ATGCTAGGCG GCTGTCAAAC CTCCACTCTA ATACTTCTCT CTCCGGGTCA
  361 TCCATCCCAT GCAGGCTCAC AGGGTGTAAC AAGCGGGTGT TCTCTCCTTC ATTGGCTTCT
  421 TCTACCTTCT CTTGCTCAAC TGGTACTAGC TTGTAGCACC ATCCAAAGGT CAGTGGATAT
  481 CTGATCCCTG GCCCTGGTGT GTAGTTCTGC CAATCAGGGA AGTAGCCTTG TGTGTGGTAG
  541 ATCCACAGAT CAAGGATATC TTGTCTTCGT TGGGAGTGAA TTAGCCCTTC CAGTCCCCCC
  601 TTTTCTTTTA AAAAGTGGCT AAGATCTACA GCTGCCTTGT AAGTCATTGG TCTTAAAGGT
  661 ACCTGAGGTG TGACTGGAAA ACCCACCTCC TCCTCCTCTT GTGCTTCTAG CCAGGCACAA
  721 TCAGCATTGG TAGCTGCTGT ATTGCTACTT GTGATTGCTC CATGTTTTTC TAGGTCTCGA
  781 CGGTATCGAT GCGGGGAGGC GGCCCAAAGG GAGATCCGAC TCGTCTGAGG GCGAAGGCGA
  841 AGACGCGGAA GAGGCCGCAG AGCCGGCAGC AGCCGCGGGA AGGAAAGGTC CGCTGGATTG
  901 AGGGCCGATG GGACGTAGCA GAAGGACGTC CCGCGCAGAA TCCAGGTGGC AACACAGGCG
  961 AGCAGCCATG GAAAGGACGT CAGCTTTCCC CGACAACACC ACGGGAATTG GTCAGTGCCC
 1021 ACAGCCGAGC CCCTGGTCCA AGCAGCGGCA GCCAGGCGCG AATGAGTACG TCGTTGCCAT
1081 AGGAGGGGCA AAGCCGGA
//
Primer: SV40pro_F RDB12274_A6Gdf.seq
>05747_12274_A6Gd_SV40pro_F_G12_21.ab1
    1 GCTGGTCCCT TTGACATCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
   61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAGCACGTGT TGACAATTAA
  121 TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA AACCATGGCC
  181 AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG TCGCCGGAGC GGTCGAGTTC
  241 TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG ACGACTTCGC CGGTGTGGTC
  301 CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC AGGTGGTGCC GGACAACACC
  361 CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG CCGAGTGGTC GGAGGTCGTG
  421 TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG AGATCGGCGA GCAGCCGTGG
  481 GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG TGCACTTCGT GGCCGAGGAG
  541 CAGGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC
  601 TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG
  661 GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT
  721 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC
  781 AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG
  841 TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC
  901 ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA
  961 TTAAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGAA ACCCTGGTCG TGCCAGCTGC
 1021 CATTAAATGA ATCGCCAAAC GCCGCGGGAG AGGGCGATTG CGTATTGGCG CTCTTTCCGC
1081 TTTTC
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

References

user report Sasaki, M., Equine major histocompatibility complex class I molecules act as entry receptors that bind to equine herpesvirus-1 glycoprotein D. Genes Cells, 16 (4), 343-357 (2011). PMID 21306483.

2017.10.23

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