Please review the QC test results indicated by icon below as well as clone information before placing your order.

CS-RfA-CMV-mRFP1

Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with mRFP1 marker driven by CMV promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering	Please visit Ordering instruction.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Remarks	Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone. This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。 提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12274_A6Gdp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Cm_M_R Sequence file: RDB12274_A6Gda.seq
>05747_12274_A6Gd_Cm_M_R_D02_11.ab1     1 GCTTGAAAAG ACCTATCCAT ATCACCAGCT CACCGTCTTT CATTGCCATA CGGAATTCCG    61 GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC TTGTGCTTAT   121 TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG TTATAGGTAC   181 ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG GATATATCAA   241 CGGTGGTATA TCCAGTGATT TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT GAAAATCTCG   301 ACGGATCCTA ACTCAAAATC CACACATTAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG   361 GGGTGCCTAA TGCGGCCGCC ATAGTGACTG GATATGTTGT GTTTTACAGT ATTATGTAGT   421 CTGTTTTTTA TGCAAAATCT AATTTAATAT ATTGATATTT ATATCATTTT ACGTTTCTCG   481 TTCAGCTTTT TTGTACAAAC TTGTGATAAT TCAAAGTGAA TTTCTGCTGT CCCTGTAATA   541 AACCCGAAAA TTTTGAATTT TTGTAATTTG TTTTTGTAAT TCTTTAGTTT GTATGTCTGT   601 TGCTATTATG TCTACTATTC TTTCCCCTGC ACTGTACCCC CCAATCCCCC CTTTTCTTTT   661 AAAATTGTGG ATGAATACTG CCATTTGTAG ATCCGTTCAC TAATCGAATG GATCTGTCTC   721 TGTCTCTCTC TCCACCTTCT TCTTCTATTC CTTCGGGCCT GTCGGGTCCC CTCGGGGTTG   781 GGAGGTGGGT CTGAAACGAT AATGGTGAAT ATCCCTGCCT AACTCTATTC ACTATAGAAA   841 AGTACAGCAA AAAACTATTC TTAAACCTAC CAAGCCCTCC TACTATCATT ATGAATAATT   901 TTATATACCA CAGCCCAATT TGTTATGTTA AACCAATTCC ACAAACTTGC CCATTTATCT   961 AATTCCCAAT AATTTCTTGT TCATTCTTTT CTTGCTGGGT TTTTGCGATT CTTCAATTAA  1021 GAGTGTAATT TAAGCTTTGT GGTATGATAA TTTCTTCTTG ATCCCACTCC AATCAAGGTC 1081 GATGTGATTC CAATCTGGAT TTCG //
Primer: Cm_C_F Sequence file: RDB12274_A6Gdb.seq
>05747_12274_A6Gd_Cm-C_F_C12_09.ab1     1 GGGCCTAACC GTGGTCCGGG GCTTACTAAA GCCAGATAAC AGTATGCGTA TTTGCGCGCT    61 GATTTTTGCG GTATAAGAAT ATATACTGAT ATGTATACCC GAAGTATGTC AAAAAGAGGT   121 ATGCTATGAA GCAGCGTATT ACAGTGACAG TTGACAGCGA CAGCTATCAG TTGCTCAAGG   181 CATATATGAT GTCAATATCT CCGGTCTGGT AAGCACAACC ATGCAGAATG AAGCCCGTCG   241 TCTGCGTGCC GAACGCTGGA AAGCGGAAAA TCAGGAAGGG ATGGCTGAGG TCGCCCGGTT   301 TATTGAAATG AACGGCTCTT TTGCTGACGA GAACAGGGGC TGGTGAAATG CAGTTTAAGG   361 TTTACACCTA TAAAAGAGAG AGCCGTTATC GTCTGTTTGT GGATGTACAG AGTGATATTA   421 TTGACACGCC CGGGCGACGG ATGGTGATCC CCCTGGCCAG TGCACGTCTG CTGTCAGATA   481 AAGTCTCCCG TGAACTTTAC CCGGTGGTGC ATATCGGGGA TGAAAGCTGG CGCATGATGA   541 CCACCGATAT GGCCAGTGTG CCGGTCTCCG TTATCGGGGA AGAAGTGGCT GATCTCAGCC   601 ACCGCGAAAA TGACATCAAA AACGCCATTA ACCTGATGTT CTGGGGAATA TAAATGTCAG   661 GCTCCCTTAT ACACAGCCAG TCTGCAGGTC GACCATAGTG ACTGGATATG TTGTGTTTTA   721 CAGTATTATG TAGTCTGTTT TTTATGCAAA ATCTAAATTT AAATATATTG ATATTTATAT   781 CATTTTACGT TTCTCGTTCA GCTTTCTTGT ACAAAGTGGT GATAATTCGA TTAATAGTAA   841 TCAATTTACG GGGTCATTAG TTCATAGCCC ATATATGGGA GTTCCCGCGT TTACATAACT   901 TACGGTAAAA TGGCCCGCCT GGCTTGACCG CCCAACGAAC CCCCCGCCCA TTGACGTTCA   961 ATAAATGAAC GTTATGTTTC CCCATAGTAA ACGGCCAAAT TAGGGACTTT TCCCATTTGA  1021 CGGTTCAATG GGGTTGGAGG TTATTTTTTA CGGTTAAACC TGGCCCCAAC TTGGGCCAGT  1081 ACATTCAAGT TGTATTCCAT ATTGCCCAAA GGTTACGGCC CCCCGTATTT GAACGATCCA 1141 ATTGAAACAG //
Primer: PRE_rev Sequence file: RDB12274_A6Gdc.seq
>05747_12274_A6Gd_PRE_rev_D12_12.ab1     1 AGAACCCATA GCGTAAAGGA GCAACATAGT TAAGAATACC AGTCAATCTT TCACAAATTT    61 TGTAATCCAG AGGTTGATTA TCGATAAGCT TGATCCCTCG AAATTCTTAG GCGCCGGTGG   121 AGTGGCGGCC CTCGGCGCGC TCGTACTGTT CCACGATGGT GTAGTCCTCG TTGTGGGAGG   181 TGATGTCCAG CTTGATGTCG GTCTTGTAGG CGCCGGGCAG CTGCACGGGC TTCTTGGCCA   241 TGTAGGTGGT CTTGACCTCG GCGTCGTAGT GGCCGCCGTC CTTCAGCTTC AGCCTCATCT   301 TGATCTCGCC CTTCAGGGCG CCGTCCTCGG GGTACATCCG CTCGGTGGAG GCCTCCCAGC   361 CCATGGTCTT CTTCTGCATT ACGGGGCCGT CGGAGGGGAA GTTGGTGCCG CGCAGCTTCA   421 CCTTGTAGAT GAACTCGCCG TCCTGCAGGG AGGAGTCCTG GGTCACGGTC ACCACGCCGC   481 CGTCCTCGAA GTTCATCACG CGCTCCCACT TGAAGCCCTC GGGGAAGGAC AGCTTCAAGT   541 AGTCGGGGAT GTCGGCGGGG TGCTTCACGT AGGCCTTGGA GCCGTACTGG AACTGAGGGG   601 ACAGGATGTC CCAGGCGAAG GGCAGGGGGC CGCCCTTGGT CACCTTCAGC TTGGCGGTCT   661 GGGTGCCCTC GTAGGGGCGG CCCTCGCCCT CGCCCTCGAT CTCGAACTCG TGGCCGTTCA   721 CGGAGCCCTC CATGCGCACC TTGAAAGCGC ATGAACTCCC TTGATGACGG TCCCTCGGAG   781 GAGGCCATTC GGGATCGCTA GCGGATCTGA ACGGGTTCAC TAAACCAGCC TCTGCTTATA   841 TAAGACCTTC CCAACGTAAC ACGCCGACCG GCCCAATTGG CGTTCAAATG GGGCGGGAGT   901 TTGTTACGAC ATTTTTGGAA AGGTCCCCGG TGAATTTTGG GTGCCCAAAA ACAACTTCCC   961 CATTGACCGT CAATGGGGGT GGAGTACTTT GGGAATAACC CCCGGTGGAA GTCCAAAACC 1021 CGACCTATAT CCGCCACGCC CGCAATGTGA //
Primer: HIVgp_R1 Sequence file: RDB12274_A6Gdd.seq
>05747_12274_A6Gd_HIVgp_R1_E12_15.ab1     1 CCGGACATTC TATTTCCCAC TGCTCTTTTT TCTCTCTGCA CCACTCTTCT CTTTGCCTTG    61 GTGGGTGCTA CTCCTAATGG TTCAATTTTT ACTACTTTAT ATTTATATAA TTCACTTCTC   121 CAATTGTCCC TCATATCTCC TCCTCCAGGT CTGAAGATCA GCGGCCGCTT GCTGTGCGGT   181 GGTCTTACTT TTGTTTTGCT CTTCCTCTAT CTTGTCTAAA GCTTCCTTGG TGTCTTTTAT   241 CTCTATCCTT TGATGCACAC AATAGAGGGT TGCTACTGTA TTATATAATG ATCTAAGTTC   301 TTCTGATCCT GTCTGAAGGG ATGGTTGTAG CTGTCCCAGT ATTTGTCTAC AGCCTTCTGA   361 TGTTTCTAAC AGGCCAGGAT TAACTGCGAA TCGTTCTAGC TCCCTGCTTG CCCATACTAT   421 ATGTTTTAAT TTATATTTTT TCTTTCCCCC TGGCCTTAAC CGAATTTTTT CCCATCGCGA   481 TCTAATTCTC CCCCGCTTAA TACTGACGCT CTCGCACCCA TCTCTCTCCT TCTAGCCTCC   541 GCTAGTCAAA ATTTTTGGCG TACTCACCAG TCGCCGCCCC TCGCCTCTTG CCGTGCGCGC   601 TTCAGCAAGC CGAGTCCTGC GTCGAGAGAG CTCCTCTGGT TTCCCTTTCG CTTTCAAGTC   661 CCTGTTCGGG CGCCACTGCT AGAGATTTTC CACACTGACT AAAAGGGTCT GAGGGATCTC   721 TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT GAAGCACTCA AGGCAAGCTT   781 TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA GCTCCCAGGC TCAGATCTGG   841 TCTAACCAGA GAGACCCAGT ACAGGCAAAA CGCGCTGCTT ATATAGACCT CCCACCGTAC   901 ACGCCTACCG CCCATTTGCG TCAATGGGGG CGGAGTTGTT ACGACATTTT GGAAAGTCCC   961 GTTTGATTTT GGGTGCCCAA AACAAACTCC CATTGAACGT CAATGGGGTG GAGACTTTGG  1021 AAAATCCCCC GTGAGTCAAA CCGGCTATCC ACGCCCATTG GATGTACTTG CCAAAATCGC 1081 AATTCATCAT GGTTAAATAA GCGATTGACT AAATAACCGT //
Primer: BGH_rev2 Sequence file: RDB12274_A6Gde.seq
>05747_12274_A6Gd_BGH_rev2_F12_18.ab1     1 CATCGGGTTA GTAGCACGGG GGAGGGGCAA CAACAGATGG CTGGCAACTA GAAGGCACAG    61 TCGAGGCTGA TCAGCGGGTT TAAACGGGCC CTGCTAGAGA TTTTCCACAC TGACTAAAAG   121 GGTCTGAGGG ATCTCTAGTT ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC   181 ACTCAAGGCA AGCTTTATTG AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC   241 CAGGCTCAGA TCTGGTCTAA CCAGAGAGAC CCAGTACAGT CCGGATGCAG CTCTCGGGCC   301 ATGTGATGAA ATGCTAGGCG GCTGTCAAAC CTCCACTCTA ATACTTCTCT CTCCGGGTCA   361 TCCATCCCAT GCAGGCTCAC AGGGTGTAAC AAGCGGGTGT TCTCTCCTTC ATTGGCTTCT   421 TCTACCTTCT CTTGCTCAAC TGGTACTAGC TTGTAGCACC ATCCAAAGGT CAGTGGATAT   481 CTGATCCCTG GCCCTGGTGT GTAGTTCTGC CAATCAGGGA AGTAGCCTTG TGTGTGGTAG   541 ATCCACAGAT CAAGGATATC TTGTCTTCGT TGGGAGTGAA TTAGCCCTTC CAGTCCCCCC   601 TTTTCTTTTA AAAAGTGGCT AAGATCTACA GCTGCCTTGT AAGTCATTGG TCTTAAAGGT   661 ACCTGAGGTG TGACTGGAAA ACCCACCTCC TCCTCCTCTT GTGCTTCTAG CCAGGCACAA   721 TCAGCATTGG TAGCTGCTGT ATTGCTACTT GTGATTGCTC CATGTTTTTC TAGGTCTCGA   781 CGGTATCGAT GCGGGGAGGC GGCCCAAAGG GAGATCCGAC TCGTCTGAGG GCGAAGGCGA   841 AGACGCGGAA GAGGCCGCAG AGCCGGCAGC AGCCGCGGGA AGGAAAGGTC CGCTGGATTG   901 AGGGCCGATG GGACGTAGCA GAAGGACGTC CCGCGCAGAA TCCAGGTGGC AACACAGGCG   961 AGCAGCCATG GAAAGGACGT CAGCTTTCCC CGACAACACC ACGGGAATTG GTCAGTGCCC  1021 ACAGCCGAGC CCCTGGTCCA AGCAGCGGCA GCCAGGCGCG AATGAGTACG TCGTTGCCAT 1081 AGGAGGGGCA AAGCCGGA //
Primer: SV40pro_F Sequence file: RDB12274_A6Gdf.seq
>05747_12274_A6Gd_SV40pro_F_G12_21.ab1     1 GCTGGTCCCT TTGACATCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA    61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAGCACGTGT TGACAATTAA   121 TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA AACCATGGCC   181 AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG TCGCCGGAGC GGTCGAGTTC   241 TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG ACGACTTCGC CGGTGTGGTC   301 CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC AGGTGGTGCC GGACAACACC   361 CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG CCGAGTGGTC GGAGGTCGTG   421 TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG AGATCGGCGA GCAGCCGTGG   481 GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG TGCACTTCGT GGCCGAGGAG   541 CAGGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC   601 TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG   661 GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT   721 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC   781 AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG   841 TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC   901 ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA   961 TTAAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGAA ACCCTGGTCG TGCCAGCTGC  1021 CATTAAATGA ATCGCCAAAC GCCGCGGGAG AGGGCGATTG CGTATTGGCG CTCTTTCCGC 1081 TTTTC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.18

GNP_filter3_RDBDEP_html_220516.pl