Please review the QC test results indicated by icon below as well as clone information before placing your order.

CS-RfA-EF-mRFP1

Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with mRFP1 marker driven by EF-1 alpha promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering	Please visit Ordering instruction.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Remarks	Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone. This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。 提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Materials & Methods section:

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13314_A5Dnp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F () Sequence file: RDB13314_A5Dna.seq
>05736_13314_A5Dn_EF1a-C_F_D02_04_ABI08.ab1     1 CTTTTGGGAT TTGCCCTTTT TGAGTTTGGA TCTTGGTTCA TTCTCAAGCC TCAGACAGTG    61 GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAATTA GATCCGGCGA GGGCAGAGGA   121 AGTCTTCTAA CATGCGGTGA CGTGGAGGAA AATCCCGGGC CTATGGCCTC CTCCGAGGAC   181 GTCATCAAGG AGTTCATGCG CTTCAAGGTG CGCATGGAGG GCTCCGTGAA CGGCCACGAG   241 TTCGAGATCG AGGGCGAGGG CGAGGGCCGC CCCTACGAGG GCACCCAGAC CGCCAAGCTG   301 AAGGTGACCA AGGGCGGCCC CCTGCCCTTC GCCTGGGACA TCCTGTCCCC TCAGTTCCAG   361 TACGGCTCCA AGGCCTACGT GAAGCACCCC GCCGACATCC CCGACTACTT GAAGCTGTCC   421 TTCCCCGAGG GCTTCAAGTG GGAGCGCGTG ATGAACTTCG AGGACGGCGG CGTGGTGACC   481 GTGACCCAGG ACTCCTCCCT GCAGGACGGC GAGTTCATCT ACAAGGTGAA GCTGCGCGGC   541 ACCAACTTCC CCTCCGACGG CCCCGTAATG CAGAAGAAGA CCATGGGCTG GGAGGCCTCC   601 ACCGAGCGGA TGTACCCCGA GGACGGCGCC CTGAAGGGCG AGATCAAGAT GAGGCTGAAG   661 CTGAAGGACG GCGGCCACTA CGACGCCGAG GTCAAGACCA CCTACATGGC CAAGAAGCCC   721 GTGCAGCTGC CCGGCGCCTA CAAGACCGAC ATCAAGCTGG ACATCACCTC CCACAACGAG   781 GACTACACCA TCGTGGAACA GTACGAGCGC GCCGAGGGCC GCCACTCCAC CGGCGCCTAA   841 GCGGCCGCAC TCGAGGGATC AAGCTTATCG ATAATCAACC TCTGGATTAC AAAATTTGTG   901 AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT   961 TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC CTCCCTTGTA  1021 TAAATCCCTG ATTGCTGTCT CTTTATGAGG AGTTGTGGGC CCCGTTTGTC AGGCAACCGT  1081 GACGTGTGTG CAACTGTGTT GCCTGAACGC ACCCCCAACT GATGGGCAAT TGCCACCCAC 1141 CATGTTCAAA GCT //
Primer: Cm_M_R () Sequence file: RDB13314_A5Dnb.seq
>05736_13314_A5Dn_Cm_M_R_B10_04.ab1     1 GTAGAGGTGA CCTATCCCAT ATCACCAGCT CACCGTCTTT CATTGCCATA CGGAATTCCG    61 GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC TTGTGCTTAT   121 TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG TTATAGGTAC   181 ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG GATATATCAA   241 CGGTGGTATA TCCAGTGATT TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT GAAAATCTCG   301 ACGGATCCTA ACTCAAAATC CACACATTAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG   361 GGGTGCCTAA TGCGGCCGCC ATAGTGACTG GATATGTTGT GTTTTACAGT ATTATGTAGT   421 CTGTTTTTTA TGCAAAATCT AATTTAATAT ATTGATATTT ATATCATTTT ACGTTTCTCG   481 TTCAGCTTTT TTGTACAAAC TTGTGATTTC AAAGTGAATT TCTGCTGTCC CTGTAATAAA   541 CCCGAAAATT TTGAATTTTT GTAATTTGTT TTTGTAATTC TTTAGTTTGT ATGTCTGTTG   601 CTATTATGTC TACTATTCTT TCCCCTGCAC TGTACCCCCC AATCCCCCCT TTTCTTTTAA   661 AATTGTGGAT GAATACTGCC ATTTGTAGAT CCGTTCACTA ATCGAATGGA TCTGTCTCTG   721 TCTCTCTCTC CACCTTCTTC TTCTATTCCT TCGGGCCTGT CGGGTCCCCT CGGGGTTGGG   781 AGGTGGGTCT GAAACGATAA TGGTGAATAT CCCTGCCTAA CTCTATTCAC TATAGAAAGT   841 ACAGCAAAAA CTATTCTTAA ACCTACCAAG CCTCCTACTA TCATTATGAA TATTTTATAT   901 ACCACAGCAT TTGTTATGTT AACCATTCAC AAACTTGCCA TTTATCTATC ATATCTGTCA   961 TTCTTTCCTT GCTGTTTTGC GATTCTTCAA TACGAATGTA TTAGCCTTGT GTATTGTAAT  1021 TTCTCTGGTC CACTCATCCA GTCAGTGATC GAATCTGTTC CCGAATTATC GACTAGCATC 1081 AGCAGCATGT GGTGCCATGA T //
Primer: Cm_C_F () Sequence file: RDB13314_A5Dnc.seq
>05736_13314_A5Dn_Cm_C_F_E02_05_ABI08.ab1     1 GACGGTTGCG GTGGATCGGG CTTACTAAAA GCCAGATAAC AGTATGCGTA TTTGCGCGCT    61 GATTTTTGCG GTATAAGAAT ATATACTGAT ATGTATACCC GAAGTATGTC AAAAAGAGGT   121 ATGCTATGAA GCAGCGTATT ACAGTGACAG TTGACAGCGA CAGCTATCAG TTGCTCAAGG   181 CATATATGAT GTCAATATCT CCGGTCTGGT AAGCACAACC ATGCAGAATG AAGCCCGTCG   241 TCTGCGTGCC GAACGCTGGA AAGCGGAAAA TCAGGAAGGG ATGGCTGAGG TCGCCCGGTT   301 TATTGAAATG AACGGCTCTT TTGCTGACGA GAACAGGGGC TGGTGAAATG CAGTTTAAGG   361 TTTACACCTA TAAAAGAGAG AGCCGTTATC GTCTGTTTGT GGATGTACAG AGTGATATTA   421 TTGACACGCC CGGGCGACGG ATGGTGATCC CCCTGGCCAG TGCACGTCTG CTGTCAGATA   481 AAGTCTCCCG TGAACTTTAC CCGGTGGTGC ATATCGGGGA TGAAAGCTGG CGCATGATGA   541 CCACCGATAT GGCCAGTGTG CCGGTCTCCG TTATCGGGGA AGAAGTGGCT GATCTCAGCC   601 ACCGCGAAAA TGACATCAAA AACGCCATTA ACCTGATGTT CTGGGGAATA TAAATGTCAG   661 GCTCCCTTAT ACACAGCCAG TCTGCAGGTC GACCATAGTG ACTGGATATG TTGTGTTTTA   721 CAGTATTATG TAGTCTGTTT TTTATGCAAA ATCTAATTTA ATATATTGAT ATTTATATCA   781 TTTTACGTTT CTCGTTCAGC TTTCTTGTAC AAAGTGGTGA TAATTCAAGC TTCGTGAGGC   841 TCCGGTGCCC GTCAGTGGGC AGAGCGCACA TCGCCCACAG TCCCCGAGAA GTTGGGGGGG   901 AGGGGTCGGC AATTGAACCG GTGCCTAGAG AAAGGTGGCG CGGGGTAAAC TGGGAAAGTG   961 ATGTCGTGTA CTGGCTCCGC CTTTTTTCCC GAGGGTGGGG GGAGAACCCG TATATAAGTG  1021 CAGTAGTCGC CGTGAACGTT CTTTTTCGCA CGGGTTTGCC GCCAGACACA GGTAAGTGCC  1081 GTGTGTGGTT TCCCGCGGCT TGCTCATTAC GGTTATGGAC CTTGCGGTGC CTTGAATTAA 1141 CT //
Primer: CMV-Forward () Sequence file: RDB13314_A5Dnd.seq
>05736_13314_A5Dn_CMV-Forward_D10_10.ab1     1 CTTACTCCGC CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC    61 AGCGCGTTTT GCCTGTACTG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT GGGAGCTCTC   121 TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG TGCTTCAAGT   181 AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC CCTTTTAGTC   241 AGTGTGGAAA ATCTCTAGCA GTGGCGCCCG AACAGGGACT TGAAAGCGAA AGGGAAACCA   301 GAGGAGCTCT CTCGACGCAG GACTCGGCTT GCTGAAGCGC GCACGGCAAG AGGCGAGGGG   361 CGGCGACTGG TGAGTACGCC AAAAATTTTG ACTAGCGGAG GCTAGAAGGA GAGAGATGGG   421 TGCGAGAGCG TCAGTATTAA GCGGGGGAGA ATTAGATCGC GATGGGAAAA AATTCGGTTA   481 AGGCCAGGGG GAAAGAAAAA ATATAAATTA AAACATATAG TATGGGCAAG CAGGGAGCTA   541 GAACGATTCG CAGTTAATCC TGGCCTGTTA GAAACATCAG AAGGCTGTAG ACAAATACTG   601 GGACAGCTAC AACCATCCCT TCAGACAGGA TCAGAAGAAC TTAGATCATT ATATAATACA   661 GTAGCAACCC TCTATTGTGT GCATCAAAGG ATAGAGATAA AAGACACCAA GGAAGCTTTA   721 GACAAGATAG AGGAAGAGCA AAACAAAAGT AAGACCACCG CACAGCAAGC GGCCGCTGAT   781 CTTCAGACCT GGAGGAGGAG ATATGAGGGA CAATTGGAGA AGTGAATTAT ATAAATATAA   841 AGTAGTAAAA ATTGAACCAT TAGGAGTAGC ACCCACCAAA GGCAAAGAGA AGAGTGGTGC   901 AGAGAGAAAA AAAGAGCAGT GGAATAGAGC TTTGTTTCCC TTGGGTTCTT GGGGAGCAGC   961 AGAAGCACTA TGGGCGCAGC GTCATGACGC TGACGTTACA GTAGACATTA TTTGTCTGGC 1021 TATAGTGCAG CCAGCAGATC ATTGCCTGAC GCTTATTGAG CGCAACAGGC AA //
Primer: BGH_rev () Sequence file: RDB13314_A5Dne.seq
>05736_13314_A5Dn_BGH_rev_E10_13.ab1     1 CCGTAGTCGG CGTCTGCTGA GATATTCCAC ACTGGACTAA AAGGGTCTGA GGGATCTCTA    61 GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA AGCACTCAAG GCAAGCTTTA   121 TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC TCCCAGGCTC AGATCTGGTC   181 TAACCAGAGA GACCCAGTAC AGTCCGGATG CAGCTCTCGG GCCATGTGAT GAAATGCTAG   241 GCGGCTGTCA AACCTCCACT CTAATACTTC TCTCTCCGGG TCATCCATCC CATGCAGGCT   301 CACAGGGTGT AACAAGCGGG TGTTCTCTCC TTCATTGGCT TCTTCTACCT TCTCTTGCTC   361 AACTGGTACT AGCTTGTAGC ACCATCCAAA GGTCAGTGGA TATCTGATCC CTGGCCCTGG   421 TGTGTAGTTC TGCCAATCAG GGAAGTAGCC TTGTGTGTGG TAGATCCACA GATCAAGGAT   481 ATCTTGTCTT CGTTGGGAGT GAATTAGCCC TTCCAGTCCC CCCTTTTCTT TTAAAAAGTG   541 GCTAAGATCT ACAGCTGCCT TGTAAGTCAT TGGTCTTAAA GGTACCTGAG GTGTGACTGG   601 AAAACCCACC TCCTCCTCCT CTTGTGCTTC TAGCCAGGCA CAATCAGCAT TGGTAGCTGC   661 TGTATTGCTA CTTGTGATTG CTCCATGTTT TTCTAGGTCT CGACGGTATC GATGCGGGGA   721 GGCGGCCCAA AGGGAGATCC GACTCGTCTG AGGGCGAAAG GCGAAGACGC GGAAGAGCCG   781 CAGAGCCGGG CAGCAGCCGC GGGGAAGGAA GTCCGCTGGA TTGAGGGCCG AAGGACGTAG   841 CAGAATGACG TCCCCGCGCA GAATCCAGGT GGCACACAGG CGAGCAGCCA TGGAAAAGGA   901 CGTCAGCTTC CCCGACAACA CACGGGAATT GTCAGTGCCA ACAGCGAGCC CCTGTTCAAG   961 CCACCGGGCC ACGCCAGGCC GGCGATGACT TCGTCGTGCA ATAGAAGGGG AAGCGAAGTC 1021 TGTAGGACTG AACAGGTGGA TGGCCAATTG CCCCTCA //
Primer: SV40pro_F_ () Sequence file: RDB13314_A5Dnf.seq
>05736_13314_A5Dn_SV40pro_F_F10_16.ab1     1 GCTGGTCCGT TTGCATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA    61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAGCACGTGT TGACAATTAA   121 TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA AACCATGGCC   181 AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG TCGCCGGAGC GGTCGAGTTC   241 TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG ACGACTTCGC CGGTGTGGTC   301 CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC AGGTGGTGCC GGACAACACC   361 CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG CCGAGTGGTC GGAGGTCGTG   421 TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG AGATCGGCGA GCAGCCGTGG   481 GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG TGCACTTCGT GGCCGAGGAG   541 CAGGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC   601 TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG   661 GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT   721 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC   781 AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG   841 TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC   901 ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGGTGCC TAATGAGTGA GCTAACTCAC   961 ATTAAATTGC GTTGCGCTCA CTGCCCGCTT TCCCAGTCGG GAAACCCTGT CGTGCCCAGC  1021 TGCATTTAAT GAATCGGGCC AACGCGCGGG GAGAGGCGGG TTTGCGTATT GGGCGCTCAT  1081 CGCTTTCTCG CTCACTGAAC TCGCTTGCGC TTCGATCGAT CCGGCCTGGC CGCCCGAAGT 1141 GGGATAATCA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.02

GNP_filter3_RDBDEP_html_230501.pl