Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with mRFP1 marker driven by EF-1 alpha promoter.
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Other clones in our bank
External Database Discosoma sp. red fluorescent protein Reference sequence |
Sequence | RDB05736z.seq |
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Remarks, protocol and/or map (pdf) | RDB05736.pdf |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution | The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer. |
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Ordering | Please visit Ordering instruction.[link] Order form [Credit Card Payment ![]() ![]() Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word ![]() |
Remarks | Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone. This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. |
提供条件 | 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。 |
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提供依頼 | 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。 提供依頼書 [Word ![]() 提供同意書 (非営利機関による非営利学術研究用)[Word ![]() 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 |
備考 | このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB05736 | CS-RfA-EF-mRFP1 | DNA solution |
Materials & Methods section:
The CS-RfA-EF-mRFP1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05736). |
Reference section:
Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Lentivirus Vector (English text) |
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Featured content | Lentivirus Vector (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB13314_A5Dnp1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: EF1a-C_F () Sequence file: RDB13314_A5Dna.seq ![]() |
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>05736_13314_A5Dn_EF1a-C_F_D02_04_ABI08.ab1 1 CTTTTGGGAT TTGCCCTTTT TGAGTTTGGA TCTTGGTTCA TTCTCAAGCC TCAGACAGTG 61 GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAATTA GATCCGGCGA GGGCAGAGGA 121 AGTCTTCTAA CATGCGGTGA CGTGGAGGAA AATCCCGGGC CTATGGCCTC CTCCGAGGAC 181 GTCATCAAGG AGTTCATGCG CTTCAAGGTG CGCATGGAGG GCTCCGTGAA CGGCCACGAG 241 TTCGAGATCG AGGGCGAGGG CGAGGGCCGC CCCTACGAGG GCACCCAGAC CGCCAAGCTG 301 AAGGTGACCA AGGGCGGCCC CCTGCCCTTC GCCTGGGACA TCCTGTCCCC TCAGTTCCAG 361 TACGGCTCCA AGGCCTACGT GAAGCACCCC GCCGACATCC CCGACTACTT GAAGCTGTCC 421 TTCCCCGAGG GCTTCAAGTG GGAGCGCGTG ATGAACTTCG AGGACGGCGG CGTGGTGACC 481 GTGACCCAGG ACTCCTCCCT GCAGGACGGC GAGTTCATCT ACAAGGTGAA GCTGCGCGGC 541 ACCAACTTCC CCTCCGACGG CCCCGTAATG CAGAAGAAGA CCATGGGCTG GGAGGCCTCC 601 ACCGAGCGGA TGTACCCCGA GGACGGCGCC CTGAAGGGCG AGATCAAGAT GAGGCTGAAG 661 CTGAAGGACG GCGGCCACTA CGACGCCGAG GTCAAGACCA CCTACATGGC CAAGAAGCCC 721 GTGCAGCTGC CCGGCGCCTA CAAGACCGAC ATCAAGCTGG ACATCACCTC CCACAACGAG 781 GACTACACCA TCGTGGAACA GTACGAGCGC GCCGAGGGCC GCCACTCCAC CGGCGCCTAA 841 GCGGCCGCAC TCGAGGGATC AAGCTTATCG ATAATCAACC TCTGGATTAC AAAATTTGTG 901 AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT 961 TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC CTCCCTTGTA 1021 TAAATCCCTG ATTGCTGTCT CTTTATGAGG AGTTGTGGGC CCCGTTTGTC AGGCAACCGT 1081 GACGTGTGTG CAACTGTGTT GCCTGAACGC ACCCCCAACT GATGGGCAAT TGCCACCCAC 1141 CATGTTCAAA GCT // |
Primer: Cm_M_R () Sequence file: RDB13314_A5Dnb.seq ![]() |
>05736_13314_A5Dn_Cm_M_R_B10_04.ab1 1 GTAGAGGTGA CCTATCCCAT ATCACCAGCT CACCGTCTTT CATTGCCATA CGGAATTCCG 61 GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC TTGTGCTTAT 121 TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG TTATAGGTAC 181 ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG GATATATCAA 241 CGGTGGTATA TCCAGTGATT TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT GAAAATCTCG 301 ACGGATCCTA ACTCAAAATC CACACATTAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG 361 GGGTGCCTAA TGCGGCCGCC ATAGTGACTG GATATGTTGT GTTTTACAGT ATTATGTAGT 421 CTGTTTTTTA TGCAAAATCT AATTTAATAT ATTGATATTT ATATCATTTT ACGTTTCTCG 481 TTCAGCTTTT TTGTACAAAC TTGTGATTTC AAAGTGAATT TCTGCTGTCC CTGTAATAAA 541 CCCGAAAATT TTGAATTTTT GTAATTTGTT TTTGTAATTC TTTAGTTTGT ATGTCTGTTG 601 CTATTATGTC TACTATTCTT TCCCCTGCAC TGTACCCCCC AATCCCCCCT TTTCTTTTAA 661 AATTGTGGAT GAATACTGCC ATTTGTAGAT CCGTTCACTA ATCGAATGGA TCTGTCTCTG 721 TCTCTCTCTC CACCTTCTTC TTCTATTCCT TCGGGCCTGT CGGGTCCCCT CGGGGTTGGG 781 AGGTGGGTCT GAAACGATAA TGGTGAATAT CCCTGCCTAA CTCTATTCAC TATAGAAAGT 841 ACAGCAAAAA CTATTCTTAA ACCTACCAAG CCTCCTACTA TCATTATGAA TATTTTATAT 901 ACCACAGCAT TTGTTATGTT AACCATTCAC AAACTTGCCA TTTATCTATC ATATCTGTCA 961 TTCTTTCCTT GCTGTTTTGC GATTCTTCAA TACGAATGTA TTAGCCTTGT GTATTGTAAT 1021 TTCTCTGGTC CACTCATCCA GTCAGTGATC GAATCTGTTC CCGAATTATC GACTAGCATC 1081 AGCAGCATGT GGTGCCATGA T // |
Primer: Cm_C_F () Sequence file: RDB13314_A5Dnc.seq ![]() |
>05736_13314_A5Dn_Cm_C_F_E02_05_ABI08.ab1 1 GACGGTTGCG GTGGATCGGG CTTACTAAAA GCCAGATAAC AGTATGCGTA TTTGCGCGCT 61 GATTTTTGCG GTATAAGAAT ATATACTGAT ATGTATACCC GAAGTATGTC AAAAAGAGGT 121 ATGCTATGAA GCAGCGTATT ACAGTGACAG TTGACAGCGA CAGCTATCAG TTGCTCAAGG 181 CATATATGAT GTCAATATCT CCGGTCTGGT AAGCACAACC ATGCAGAATG AAGCCCGTCG 241 TCTGCGTGCC GAACGCTGGA AAGCGGAAAA TCAGGAAGGG ATGGCTGAGG TCGCCCGGTT 301 TATTGAAATG AACGGCTCTT TTGCTGACGA GAACAGGGGC TGGTGAAATG CAGTTTAAGG 361 TTTACACCTA TAAAAGAGAG AGCCGTTATC GTCTGTTTGT GGATGTACAG AGTGATATTA 421 TTGACACGCC CGGGCGACGG ATGGTGATCC CCCTGGCCAG TGCACGTCTG CTGTCAGATA 481 AAGTCTCCCG TGAACTTTAC CCGGTGGTGC ATATCGGGGA TGAAAGCTGG CGCATGATGA 541 CCACCGATAT GGCCAGTGTG CCGGTCTCCG TTATCGGGGA AGAAGTGGCT GATCTCAGCC 601 ACCGCGAAAA TGACATCAAA AACGCCATTA ACCTGATGTT CTGGGGAATA TAAATGTCAG 661 GCTCCCTTAT ACACAGCCAG TCTGCAGGTC GACCATAGTG ACTGGATATG TTGTGTTTTA 721 CAGTATTATG TAGTCTGTTT TTTATGCAAA ATCTAATTTA ATATATTGAT ATTTATATCA 781 TTTTACGTTT CTCGTTCAGC TTTCTTGTAC AAAGTGGTGA TAATTCAAGC TTCGTGAGGC 841 TCCGGTGCCC GTCAGTGGGC AGAGCGCACA TCGCCCACAG TCCCCGAGAA GTTGGGGGGG 901 AGGGGTCGGC AATTGAACCG GTGCCTAGAG AAAGGTGGCG CGGGGTAAAC TGGGAAAGTG 961 ATGTCGTGTA CTGGCTCCGC CTTTTTTCCC GAGGGTGGGG GGAGAACCCG TATATAAGTG 1021 CAGTAGTCGC CGTGAACGTT CTTTTTCGCA CGGGTTTGCC GCCAGACACA GGTAAGTGCC 1081 GTGTGTGGTT TCCCGCGGCT TGCTCATTAC GGTTATGGAC CTTGCGGTGC CTTGAATTAA 1141 CT // |
Primer: CMV-Forward () Sequence file: RDB13314_A5Dnd.seq ![]() |
>05736_13314_A5Dn_CMV-Forward_D10_10.ab1 1 CTTACTCCGC CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC 61 AGCGCGTTTT GCCTGTACTG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT GGGAGCTCTC 121 TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG TGCTTCAAGT 181 AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC CCTTTTAGTC 241 AGTGTGGAAA ATCTCTAGCA GTGGCGCCCG AACAGGGACT TGAAAGCGAA AGGGAAACCA 301 GAGGAGCTCT CTCGACGCAG GACTCGGCTT GCTGAAGCGC GCACGGCAAG AGGCGAGGGG 361 CGGCGACTGG TGAGTACGCC AAAAATTTTG ACTAGCGGAG GCTAGAAGGA GAGAGATGGG 421 TGCGAGAGCG TCAGTATTAA GCGGGGGAGA ATTAGATCGC GATGGGAAAA AATTCGGTTA 481 AGGCCAGGGG GAAAGAAAAA ATATAAATTA AAACATATAG TATGGGCAAG CAGGGAGCTA 541 GAACGATTCG CAGTTAATCC TGGCCTGTTA GAAACATCAG AAGGCTGTAG ACAAATACTG 601 GGACAGCTAC AACCATCCCT TCAGACAGGA TCAGAAGAAC TTAGATCATT ATATAATACA 661 GTAGCAACCC TCTATTGTGT GCATCAAAGG ATAGAGATAA AAGACACCAA GGAAGCTTTA 721 GACAAGATAG AGGAAGAGCA AAACAAAAGT AAGACCACCG CACAGCAAGC GGCCGCTGAT 781 CTTCAGACCT GGAGGAGGAG ATATGAGGGA CAATTGGAGA AGTGAATTAT ATAAATATAA 841 AGTAGTAAAA ATTGAACCAT TAGGAGTAGC ACCCACCAAA GGCAAAGAGA AGAGTGGTGC 901 AGAGAGAAAA AAAGAGCAGT GGAATAGAGC TTTGTTTCCC TTGGGTTCTT GGGGAGCAGC 961 AGAAGCACTA TGGGCGCAGC GTCATGACGC TGACGTTACA GTAGACATTA TTTGTCTGGC 1021 TATAGTGCAG CCAGCAGATC ATTGCCTGAC GCTTATTGAG CGCAACAGGC AA // |
Primer: BGH_rev () Sequence file: RDB13314_A5Dne.seq ![]() |
>05736_13314_A5Dn_BGH_rev_E10_13.ab1 1 CCGTAGTCGG CGTCTGCTGA GATATTCCAC ACTGGACTAA AAGGGTCTGA GGGATCTCTA 61 GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA AGCACTCAAG GCAAGCTTTA 121 TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC TCCCAGGCTC AGATCTGGTC 181 TAACCAGAGA GACCCAGTAC AGTCCGGATG CAGCTCTCGG GCCATGTGAT GAAATGCTAG 241 GCGGCTGTCA AACCTCCACT CTAATACTTC TCTCTCCGGG TCATCCATCC CATGCAGGCT 301 CACAGGGTGT AACAAGCGGG TGTTCTCTCC TTCATTGGCT TCTTCTACCT TCTCTTGCTC 361 AACTGGTACT AGCTTGTAGC ACCATCCAAA GGTCAGTGGA TATCTGATCC CTGGCCCTGG 421 TGTGTAGTTC TGCCAATCAG GGAAGTAGCC TTGTGTGTGG TAGATCCACA GATCAAGGAT 481 ATCTTGTCTT CGTTGGGAGT GAATTAGCCC TTCCAGTCCC CCCTTTTCTT TTAAAAAGTG 541 GCTAAGATCT ACAGCTGCCT TGTAAGTCAT TGGTCTTAAA GGTACCTGAG GTGTGACTGG 601 AAAACCCACC TCCTCCTCCT CTTGTGCTTC TAGCCAGGCA CAATCAGCAT TGGTAGCTGC 661 TGTATTGCTA CTTGTGATTG CTCCATGTTT TTCTAGGTCT CGACGGTATC GATGCGGGGA 721 GGCGGCCCAA AGGGAGATCC GACTCGTCTG AGGGCGAAAG GCGAAGACGC GGAAGAGCCG 781 CAGAGCCGGG CAGCAGCCGC GGGGAAGGAA GTCCGCTGGA TTGAGGGCCG AAGGACGTAG 841 CAGAATGACG TCCCCGCGCA GAATCCAGGT GGCACACAGG CGAGCAGCCA TGGAAAAGGA 901 CGTCAGCTTC CCCGACAACA CACGGGAATT GTCAGTGCCA ACAGCGAGCC CCTGTTCAAG 961 CCACCGGGCC ACGCCAGGCC GGCGATGACT TCGTCGTGCA ATAGAAGGGG AAGCGAAGTC 1021 TGTAGGACTG AACAGGTGGA TGGCCAATTG CCCCTCA // |
Primer: SV40pro_F_ () Sequence file: RDB13314_A5Dnf.seq ![]() |
>05736_13314_A5Dn_SV40pro_F_F10_16.ab1 1 GCTGGTCCGT TTGCATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA 61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAGCACGTGT TGACAATTAA 121 TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA AACCATGGCC 181 AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG TCGCCGGAGC GGTCGAGTTC 241 TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG ACGACTTCGC CGGTGTGGTC 301 CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC AGGTGGTGCC GGACAACACC 361 CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG CCGAGTGGTC GGAGGTCGTG 421 TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG AGATCGGCGA GCAGCCGTGG 481 GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG TGCACTTCGT GGCCGAGGAG 541 CAGGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC 601 TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG 661 GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT 721 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC 781 AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG 841 TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC 901 ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGGTGCC TAATGAGTGA GCTAACTCAC 961 ATTAAATTGC GTTGCGCTCA CTGCCCGCTT TCCCAGTCGG GAAACCCTGT CGTGCCCAGC 1021 TGCATTTAAT GAATCGGGCC AACGCGCGGG GAGAGGCGGG TTTGCGTATT GGGCGCTCAT 1081 CGCTTTCTCG CTCACTGAAC TCGCTTGCGC TTCGATCGAT CCGGCCTGGC CGCCCGAAGT 1141 GGGATAATCA // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
user_report | Matsuda, Y., Epigenetic Heterogeneity in HIV-1 Latency Establishment. Sci. Rep. 5: 7701 (2015). PMID 25572573. |
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2023.05.02
GNP_filter3_RDBDEP_html_230501.pl