Resource data sheet
Prev. |  Lentiviral vector plasmid > 
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CS-RfA-EF-mRFP1

Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with mRFP1 marker driven by EF-1 alpha promoter.

Catalog number RDB05736
Resource name CS-RfA-EF-mRFP1
Clone info. Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with mRFP1 marker driven by EF-1 alpha promoter.
Comment Commonly requested with pENTR4-H1 (RDB04395) for generation of shRNA expression vector and with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394) for packaging. Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone Plasmid
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Gene/insert name Discosoma sp. red fluorescent protein cDNA
Depositor|Developer Miyoshi, Hiroyuki |
Other clones in our bank

External Database
Discosoma sp. red fluorescent protein

            Reference sequence
              
            Lentiviral plasmid list. [link]

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
            Ordering Please visit Ordering instruction.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
            Remarks Remember that you will be working with samples containing infectious virus.
            Use Survival2 (Invitrogen) or equivalent to amplify this clone.
            This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
            提供案内 (日本国内) [open/close]

            提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
            提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (非営利機関による非営利学術研究用)[Word]
            遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
            備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
            このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。
            Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB05736 CS-RfA-EF-mRFP1 DNA solution

            Ordering instruction of plasmids [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB05736z.seq
            Remarks, protocol and/or map (pdf) RDB05736.pdf

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Lentivirus Vector (English text)
            Featured content Lentivirus Vector (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13314_A5Dnp1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: EF1a-C_F
            Sequence file: RDB13314_A5Dna.seq check
            >05736_13314_A5Dn_EF1a-C_F_D02_04_ABI08.ab1
                1 CTTTTGGGAT TTGCCCTTTT TGAGTTTGGA TCTTGGTTCA TTCTCAAGCC TCAGACAGTG
               61 GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAATTA GATCCGGCGA GGGCAGAGGA
              121 AGTCTTCTAA CATGCGGTGA CGTGGAGGAA AATCCCGGGC CTATGGCCTC CTCCGAGGAC
              181 GTCATCAAGG AGTTCATGCG CTTCAAGGTG CGCATGGAGG GCTCCGTGAA CGGCCACGAG
              241 TTCGAGATCG AGGGCGAGGG CGAGGGCCGC CCCTACGAGG GCACCCAGAC CGCCAAGCTG
              301 AAGGTGACCA AGGGCGGCCC CCTGCCCTTC GCCTGGGACA TCCTGTCCCC TCAGTTCCAG
              361 TACGGCTCCA AGGCCTACGT GAAGCACCCC GCCGACATCC CCGACTACTT GAAGCTGTCC
              421 TTCCCCGAGG GCTTCAAGTG GGAGCGCGTG ATGAACTTCG AGGACGGCGG CGTGGTGACC
              481 GTGACCCAGG ACTCCTCCCT GCAGGACGGC GAGTTCATCT ACAAGGTGAA GCTGCGCGGC
              541 ACCAACTTCC CCTCCGACGG CCCCGTAATG CAGAAGAAGA CCATGGGCTG GGAGGCCTCC
              601 ACCGAGCGGA TGTACCCCGA GGACGGCGCC CTGAAGGGCG AGATCAAGAT GAGGCTGAAG
              661 CTGAAGGACG GCGGCCACTA CGACGCCGAG GTCAAGACCA CCTACATGGC CAAGAAGCCC
              721 GTGCAGCTGC CCGGCGCCTA CAAGACCGAC ATCAAGCTGG ACATCACCTC CCACAACGAG
              781 GACTACACCA TCGTGGAACA GTACGAGCGC GCCGAGGGCC GCCACTCCAC CGGCGCCTAA
              841 GCGGCCGCAC TCGAGGGATC AAGCTTATCG ATAATCAACC TCTGGATTAC AAAATTTGTG
              901 AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT
              961 TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC CTCCCTTGTA
             1021 TAAATCCCTG ATTGCTGTCT CTTTATGAGG AGTTGTGGGC CCCGTTTGTC AGGCAACCGT
             1081 GACGTGTGTG CAACTGTGTT GCCTGAACGC ACCCCCAACT GATGGGCAAT TGCCACCCAC
            1141 CATGTTCAAA GCT
            //
            Primer: Cm_M_R
            Sequence file: RDB13314_A5Dnb.seq check
            >05736_13314_A5Dn_Cm_M_R_B10_04.ab1
                1 GTAGAGGTGA CCTATCCCAT ATCACCAGCT CACCGTCTTT CATTGCCATA CGGAATTCCG
               61 GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC TTGTGCTTAT
              121 TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG TTATAGGTAC
              181 ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG GATATATCAA
              241 CGGTGGTATA TCCAGTGATT TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT GAAAATCTCG
              301 ACGGATCCTA ACTCAAAATC CACACATTAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG
              361 GGGTGCCTAA TGCGGCCGCC ATAGTGACTG GATATGTTGT GTTTTACAGT ATTATGTAGT
              421 CTGTTTTTTA TGCAAAATCT AATTTAATAT ATTGATATTT ATATCATTTT ACGTTTCTCG
              481 TTCAGCTTTT TTGTACAAAC TTGTGATTTC AAAGTGAATT TCTGCTGTCC CTGTAATAAA
              541 CCCGAAAATT TTGAATTTTT GTAATTTGTT TTTGTAATTC TTTAGTTTGT ATGTCTGTTG
              601 CTATTATGTC TACTATTCTT TCCCCTGCAC TGTACCCCCC AATCCCCCCT TTTCTTTTAA
              661 AATTGTGGAT GAATACTGCC ATTTGTAGAT CCGTTCACTA ATCGAATGGA TCTGTCTCTG
              721 TCTCTCTCTC CACCTTCTTC TTCTATTCCT TCGGGCCTGT CGGGTCCCCT CGGGGTTGGG
              781 AGGTGGGTCT GAAACGATAA TGGTGAATAT CCCTGCCTAA CTCTATTCAC TATAGAAAGT
              841 ACAGCAAAAA CTATTCTTAA ACCTACCAAG CCTCCTACTA TCATTATGAA TATTTTATAT
              901 ACCACAGCAT TTGTTATGTT AACCATTCAC AAACTTGCCA TTTATCTATC ATATCTGTCA
              961 TTCTTTCCTT GCTGTTTTGC GATTCTTCAA TACGAATGTA TTAGCCTTGT GTATTGTAAT
             1021 TTCTCTGGTC CACTCATCCA GTCAGTGATC GAATCTGTTC CCGAATTATC GACTAGCATC
            1081 AGCAGCATGT GGTGCCATGA T
            //
            Primer: Cm_C_F
            Sequence file: RDB13314_A5Dnc.seq check
            >05736_13314_A5Dn_Cm_C_F_E02_05_ABI08.ab1
                1 GACGGTTGCG GTGGATCGGG CTTACTAAAA GCCAGATAAC AGTATGCGTA TTTGCGCGCT
               61 GATTTTTGCG GTATAAGAAT ATATACTGAT ATGTATACCC GAAGTATGTC AAAAAGAGGT
              121 ATGCTATGAA GCAGCGTATT ACAGTGACAG TTGACAGCGA CAGCTATCAG TTGCTCAAGG
              181 CATATATGAT GTCAATATCT CCGGTCTGGT AAGCACAACC ATGCAGAATG AAGCCCGTCG
              241 TCTGCGTGCC GAACGCTGGA AAGCGGAAAA TCAGGAAGGG ATGGCTGAGG TCGCCCGGTT
              301 TATTGAAATG AACGGCTCTT TTGCTGACGA GAACAGGGGC TGGTGAAATG CAGTTTAAGG
              361 TTTACACCTA TAAAAGAGAG AGCCGTTATC GTCTGTTTGT GGATGTACAG AGTGATATTA
              421 TTGACACGCC CGGGCGACGG ATGGTGATCC CCCTGGCCAG TGCACGTCTG CTGTCAGATA
              481 AAGTCTCCCG TGAACTTTAC CCGGTGGTGC ATATCGGGGA TGAAAGCTGG CGCATGATGA
              541 CCACCGATAT GGCCAGTGTG CCGGTCTCCG TTATCGGGGA AGAAGTGGCT GATCTCAGCC
              601 ACCGCGAAAA TGACATCAAA AACGCCATTA ACCTGATGTT CTGGGGAATA TAAATGTCAG
              661 GCTCCCTTAT ACACAGCCAG TCTGCAGGTC GACCATAGTG ACTGGATATG TTGTGTTTTA
              721 CAGTATTATG TAGTCTGTTT TTTATGCAAA ATCTAATTTA ATATATTGAT ATTTATATCA
              781 TTTTACGTTT CTCGTTCAGC TTTCTTGTAC AAAGTGGTGA TAATTCAAGC TTCGTGAGGC
              841 TCCGGTGCCC GTCAGTGGGC AGAGCGCACA TCGCCCACAG TCCCCGAGAA GTTGGGGGGG
              901 AGGGGTCGGC AATTGAACCG GTGCCTAGAG AAAGGTGGCG CGGGGTAAAC TGGGAAAGTG
              961 ATGTCGTGTA CTGGCTCCGC CTTTTTTCCC GAGGGTGGGG GGAGAACCCG TATATAAGTG
             1021 CAGTAGTCGC CGTGAACGTT CTTTTTCGCA CGGGTTTGCC GCCAGACACA GGTAAGTGCC
             1081 GTGTGTGGTT TCCCGCGGCT TGCTCATTAC GGTTATGGAC CTTGCGGTGC CTTGAATTAA
            1141 CT
            //
            Primer: CMV-Forward
            Sequence file: RDB13314_A5Dnd.seq check
            >05736_13314_A5Dn_CMV-Forward_D10_10.ab1
                1 CTTACTCCGC CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
               61 AGCGCGTTTT GCCTGTACTG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT GGGAGCTCTC
              121 TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG TGCTTCAAGT
              181 AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC CCTTTTAGTC
              241 AGTGTGGAAA ATCTCTAGCA GTGGCGCCCG AACAGGGACT TGAAAGCGAA AGGGAAACCA
              301 GAGGAGCTCT CTCGACGCAG GACTCGGCTT GCTGAAGCGC GCACGGCAAG AGGCGAGGGG
              361 CGGCGACTGG TGAGTACGCC AAAAATTTTG ACTAGCGGAG GCTAGAAGGA GAGAGATGGG
              421 TGCGAGAGCG TCAGTATTAA GCGGGGGAGA ATTAGATCGC GATGGGAAAA AATTCGGTTA
              481 AGGCCAGGGG GAAAGAAAAA ATATAAATTA AAACATATAG TATGGGCAAG CAGGGAGCTA
              541 GAACGATTCG CAGTTAATCC TGGCCTGTTA GAAACATCAG AAGGCTGTAG ACAAATACTG
              601 GGACAGCTAC AACCATCCCT TCAGACAGGA TCAGAAGAAC TTAGATCATT ATATAATACA
              661 GTAGCAACCC TCTATTGTGT GCATCAAAGG ATAGAGATAA AAGACACCAA GGAAGCTTTA
              721 GACAAGATAG AGGAAGAGCA AAACAAAAGT AAGACCACCG CACAGCAAGC GGCCGCTGAT
              781 CTTCAGACCT GGAGGAGGAG ATATGAGGGA CAATTGGAGA AGTGAATTAT ATAAATATAA
              841 AGTAGTAAAA ATTGAACCAT TAGGAGTAGC ACCCACCAAA GGCAAAGAGA AGAGTGGTGC
              901 AGAGAGAAAA AAAGAGCAGT GGAATAGAGC TTTGTTTCCC TTGGGTTCTT GGGGAGCAGC
              961 AGAAGCACTA TGGGCGCAGC GTCATGACGC TGACGTTACA GTAGACATTA TTTGTCTGGC
            1021 TATAGTGCAG CCAGCAGATC ATTGCCTGAC GCTTATTGAG CGCAACAGGC AA
            //
            Primer: BGH_rev
            Sequence file: RDB13314_A5Dne.seq check
            >05736_13314_A5Dn_BGH_rev_E10_13.ab1
                1 CCGTAGTCGG CGTCTGCTGA GATATTCCAC ACTGGACTAA AAGGGTCTGA GGGATCTCTA
               61 GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA AGCACTCAAG GCAAGCTTTA
              121 TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC TCCCAGGCTC AGATCTGGTC
              181 TAACCAGAGA GACCCAGTAC AGTCCGGATG CAGCTCTCGG GCCATGTGAT GAAATGCTAG
              241 GCGGCTGTCA AACCTCCACT CTAATACTTC TCTCTCCGGG TCATCCATCC CATGCAGGCT
              301 CACAGGGTGT AACAAGCGGG TGTTCTCTCC TTCATTGGCT TCTTCTACCT TCTCTTGCTC
              361 AACTGGTACT AGCTTGTAGC ACCATCCAAA GGTCAGTGGA TATCTGATCC CTGGCCCTGG
              421 TGTGTAGTTC TGCCAATCAG GGAAGTAGCC TTGTGTGTGG TAGATCCACA GATCAAGGAT
              481 ATCTTGTCTT CGTTGGGAGT GAATTAGCCC TTCCAGTCCC CCCTTTTCTT TTAAAAAGTG
              541 GCTAAGATCT ACAGCTGCCT TGTAAGTCAT TGGTCTTAAA GGTACCTGAG GTGTGACTGG
              601 AAAACCCACC TCCTCCTCCT CTTGTGCTTC TAGCCAGGCA CAATCAGCAT TGGTAGCTGC
              661 TGTATTGCTA CTTGTGATTG CTCCATGTTT TTCTAGGTCT CGACGGTATC GATGCGGGGA
              721 GGCGGCCCAA AGGGAGATCC GACTCGTCTG AGGGCGAAAG GCGAAGACGC GGAAGAGCCG
              781 CAGAGCCGGG CAGCAGCCGC GGGGAAGGAA GTCCGCTGGA TTGAGGGCCG AAGGACGTAG
              841 CAGAATGACG TCCCCGCGCA GAATCCAGGT GGCACACAGG CGAGCAGCCA TGGAAAAGGA
              901 CGTCAGCTTC CCCGACAACA CACGGGAATT GTCAGTGCCA ACAGCGAGCC CCTGTTCAAG
              961 CCACCGGGCC ACGCCAGGCC GGCGATGACT TCGTCGTGCA ATAGAAGGGG AAGCGAAGTC
            1021 TGTAGGACTG AACAGGTGGA TGGCCAATTG CCCCTCA
            //
            Primer: SV40pro_F_
            Sequence file: RDB13314_A5Dnf.seq check
            >05736_13314_A5Dn_SV40pro_F_F10_16.ab1
                1 GCTGGTCCGT TTGCATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
               61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAGCACGTGT TGACAATTAA
              121 TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA AACCATGGCC
              181 AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG TCGCCGGAGC GGTCGAGTTC
              241 TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG ACGACTTCGC CGGTGTGGTC
              301 CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC AGGTGGTGCC GGACAACACC
              361 CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG CCGAGTGGTC GGAGGTCGTG
              421 TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG AGATCGGCGA GCAGCCGTGG
              481 GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG TGCACTTCGT GGCCGAGGAG
              541 CAGGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC
              601 TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG
              661 GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT
              721 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC
              781 AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG
              841 TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC
              901 ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGGTGCC TAATGAGTGA GCTAACTCAC
              961 ATTAAATTGC GTTGCGCTCA CTGCCCGCTT TCCCAGTCGG GAAACCCTGT CGTGCCCAGC
             1021 TGCATTTAAT GAATCGGGCC AACGCGCGGG GAGAGGCGGG TTTGCGTATT GGGCGCTCAT
             1081 CGCTTTCTCG CTCACTGAAC TCGCTTGCGC TTCGATCGAT CCGGCCTGGC CGCCCGAAGT
            1141 GGGATAATCA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            user_report Matsuda, Y., Epigenetic Heterogeneity in HIV-1 Latency Establishment. Sci. Rep. 5: 7701 (2015). PMID 25572573.

            2022.05.18

            GNP_filter3_RDBDEP_html_220516.pl